Publications by authors named "Yun Zhao"

859 Publications

[Molecular chaperones facilitate soluble expression of recombinant non-toxic mutant CRM197 of diphtheria toxin in Escherichia coli].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1368-1375

Hubei Provincial Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Medicine, China Three Gorges University, Yichang 443002, Hubei, China.

Diphtheria toxin is an ADP-ribosyltransferase toxic to human cells. Mutation of the active site in its catalytic domain eliminates the toxicity, but retains its immunogenicity. A non-toxic mutant of diphtheria toxin known as CRM197 protein has become an ideal carrier protein for conjugate vaccines. CRM197 can further improve its immunogenicity by cross-linking with other antigens, so it has good potential to find broad applications. Unfortunately, inclusion bodies are easily formed during the expression of recombinant CRM197 protein in Escherichia coli, which greatly reduces its yield. In order to address this problem, pG-KJE8 vector carrying molecular chaperones and plasmid pET28a-CRM197, were co-expressed in Escherichia coli. The results showed that the recombinant CRM197 protein was successfully expressed and appeared largely in inclusion bodies. The molecular chaperones DnaK, DnaJ, GrpE, GroES and GroEL5 expressed can facilitate correct and rapid folding of CRM197. Furthermore, it can also improve the recovery rate of soluble CRM197 protein. The soluble expression of CRM197 was maximized upon addition of 1.0 mmol/L IPTG, 0.5 mg L-arabinose, 5.0 ng/mL tetracycline and induction at 20oC for 16 h. The soluble CRM197 protein shows good immunoreactivity, demonstrating the molecular chaperones expressed from pG-KJE8 facilitated the soluble expression of CRM197 protein in E. coli.
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http://dx.doi.org/10.13345/j.cjb.200569DOI Listing
April 2021

Nutritional status is closely related to the severity of COVID-19: a multi-center retrospective study.

J Infect Dev Ctries 2021 04 30;15(4):490-500. Epub 2021 Apr 30.

Department of Infectious Diseases, Taizhou People's Hospital, Taizhou, Jiangsu Province, China.

Introduction: Nutritional status has been defined as an individual's health condition. The relationship between the progression of COVID-19 and Nutritional status is still unclear. We analyzed the clinical characteristics of 342 coronavirus disease 2019 (COVID-19) patients, and analyzed the relationship between the progression of COVID-19 and Nutritional status.

Methodology: 342 COVID-19 were enrolled from ten different hospitals in China. The clinical characteristics were collected and analyzed.

Results: The body mass index (BMI) of the mild patients (Group A) was higher than those in severe patients (Group B) and critical patients (Group C); The lactate dehydrogenase (LDH) level of Group A was lower than those of the other two groups; Sex, age, and BMI, was strongly correlated with Clinical classification (CT); Among the laboratory test results, Neutrophil (NEU%), Aspartate aminotransferase (AST), LDH, and blood glucose (BG) were positively correlated with CT; Lymphocyte ( LYM%), Platelet (PLT), Albumin (ALB), and Creatinine (Cr) were negatively correlated with CT. BMI, NEU%, LYM%, ALB, Cr, and PLT are all protective factors that affect CT.

Conclusion: People with poor nutritional status (lower BMI and ALB) have a higher risk of developing severe disease after infection with SARS-CoV-2. In the clinical treatment of COVID-19, individualized nutritional support is very important for the rehabilitation of patients.
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http://dx.doi.org/10.3855/jidc.14178DOI Listing
April 2021

Evaluating the efficacy of anti-fungal blue light therapies via analyzing tissue section images.

Lasers Med Sci 2021 May 4. Epub 2021 May 4.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.

Anti-fungal blue light (ABL) therapies have been studied and applied in treating various diseases caused by fungal infection. The existing work has been mainly devoted to study the effect of various light dosages on the fungal viability and on the induced cytotoxic reactive oxygen species (ROS) in the pathogens. While in vivo experimental studies have also been reported, there is still no work targeted on quantifying the effect of light on prohibiting the pathogens from invading into the deeper sites in the skin of their host. This can be attributed to the lack of methods to analyze the tissue section images, which are the main means of examining infected tissues. This work has been devoted to solve such problems, so as to improve dosimetric analyses of ABL therapies on treating fungal infections. Specifically, the invasion depth of the fungi and their ratios to the tissue in four bins at different depths inside the skin were extracted from the tissue section images. The significance of the treatment with different dosages on inhibiting the fungi was also tested by each of these depth-related metrics. The ABL experiments using 415-nm-wavelength LED light were performed on BALB/c mice, whose skin was infected by Candida albicans (C. albicans). The proposed methods were applied to the tissue sections of the experimental animals. The results clearly verified that the fluence up to 180J/cm can significantly prohibit the fungal infection into the skin in terms of almost all the newly proposed metrics.
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http://dx.doi.org/10.1007/s10103-021-03319-9DOI Listing
May 2021

Near-field nonlinear imaging of an anapole mode beyond diffraction limit.

Opt Lett 2021 May;46(9):2095-2098

Nonlinear nanophotonics, as an emerging field in nanophotonics, eagerly calls for experimental techniques for probing and analyzing near-field nonlinear optical signals with subwavelength resolution. Here, we report an aperture-type scanning near-field optical microscopic method for probing near-field nonlinear optical processes. As a demonstration, near-field third-harmonic generation from an anapole dark-mode state generated by a silicon nanodisk is probed and imaged. The measured results agree well with the simulations, with a spatial resolution down to $0.14{\lambda _0}$ and a sensitivity of 0.1 nW. This method provides a powerful tool for characterizing nonlinear light-matter interactions at the nanoscale, which can help, for example, to unveil crystal properties involving subwavelength defects or dislocations.
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http://dx.doi.org/10.1364/OL.418664DOI Listing
May 2021

Follicular fluid exosomes: Important modulator in proliferation and steroid synthesis of porcine granulosa cells.

FASEB J 2021 May;35(5):e21610

College of Animal Sciences, Jilin University, Changchun, China.

Granulosa cells (GCs) are regulated by various factors during ovarian development. However, there are few reports on the role of follicular fluid exosomes in ovarian GCs. In this study, porcine ovarian GCs were used to explore the effects of follicular fluid exosomes on GCs. GCs were treated with in vitro, and the changes in cell proliferation, steroid synthesis, and associated signal pathways were detected. The results showed that exosomes increased cell viability and altered the gene expression profile of GCs. Exosomes also increased the level of gene expression associated with both proliferation and progesterone synthesis, in which the MAPK/ERK and WNT/B-CATENIN pathways were involved. In addition, exosome-carried microRNAs were identified by high-throughput sequencing, and exosomal miR-31-5p was found to promote the proliferation of GCs and progesterone synthesis via the WNT/B-CATENIN pathway by targeting the SFRP4 follicle growth inhibitor. In conclusion, this study has demonstrated that exosomes are essential substances involved in regulating the physiological function of GCs.
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http://dx.doi.org/10.1096/fj.202100030RRDOI Listing
May 2021

Anti-androgen therapy induces transcriptomic reprogramming in metastatic castration-resistant prostate cancer in a murine model.

Biochim Biophys Acta Mol Basis Dis 2021 Apr 21;1867(7):166151. Epub 2021 Apr 21.

Department of Biological Sciences, Boler-Parseghian Center for Rare and Neglected Diseases, Harper Cancer Research Institute, University of Notre Dame, Notre Dame, IN 46556, USA; Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indianapolis, IN 46202, USA. Electronic address:

Despite recent development of next-generation androgen receptor (AR) antagonists, metastatic castration-resistant prostate cancer (CRPC) remains incurable and requires deeper understanding through studies in suitable animal models. Prostate-specific deletion of Pten and Smad4 in mice recapitulated the disease progression of human prostate adenocarcinoma, including metastasis to lymph nodes and lung. Moreover, Pten/Smad4 tumors fostered an immunosuppressive microenvironment dominated by myeloid-derived suppressor cells (MDSCs). However, the response of Pten/Smad4 tumors to androgen deprivation and anti-androgen therapies has not been described. Here, we report that the combination of surgical castration and enzalutamide treatment in Pten/Smad4 mice slowed down the tumor growth and prolonged the median survival of the mice for 8 weeks. Treatment-naïve and castration-resistant primary tumors exhibited comparable levels of immune infiltrations with the exception of reduced monocytic MDSCs in CRPC. RNA profiling of treatment-naïve and castration-resistant primary tumors revealed largely preserved transcriptome with modest expressional alterations of collagen-related and immune-related genes, among which CC chemokine receptor type 2 (Ccr2) downregulation and predicted negative activation in CRPC was consistent with reduced monocytic MDSC infiltration. Importantly, significant transcriptomic reprograming was observed in lung metastatic CRPC compared with primary CRPC and enriched for immune-related and coagulation-related pathways. At the individual gene level, we validated the expression changes of some of the most upregulated (Cd36, Bmp5, Bmp6, Etv5, Prex2, Ptprb, Egfl6, Itga8 and Cxcl12) and downregulated genes (Cxcl9 and Adamts5). Together, this study uncovers the inherent activity of Pten/Smad4 tumors to progress to CRPC and highlights potentially targetable transcriptomic signatures associated with CRPC metastasis.
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http://dx.doi.org/10.1016/j.bbadis.2021.166151DOI Listing
April 2021

Solid Polymer Electrolytes with High Conductivity and Transference Number of Li Ions for Li-Based Rechargeable Batteries.

Adv Sci (Weinh) 2021 Apr 8;8(7):2003675. Epub 2021 Feb 8.

Department of Chemistry and Biochemistry Northern Illinois University DeKalb IL 60115 USA.

Smart electronics and wearable devices require batteries with increased energy density, enhanced safety, and improved mechanical flexibility. However, current state-of-the-art Li-based rechargeable batteries (LBRBs) use highly reactive and flowable liquid electrolytes, severely limiting their ability to meet the above requirements. Therefore, solid polymer electrolytes (SPEs) are introduced to tackle the issues of liquid electrolytes. Nevertheless, due to their low Li conductivity and Li transference number (LITN) (around 10 S cm and 0.5, respectively), SPE-based room temperature LBRBs are still in their early stages of development. This paper reviews the principles of Li conduction inside SPEs and the corresponding strategies to improve the Li conductivity and LITN of SPEs. Some representative applications of SPEs in high-energy density, safe, and flexible LBRBs are then introduced and prospected.
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http://dx.doi.org/10.1002/advs.202003675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025011PMC
April 2021

Antibiotic Use Among Hospitalized Children and Neonates in China: Results From Quarterly Point Prevalence Surveys in 2019.

Front Pharmacol 2021 29;12:601561. Epub 2021 Mar 29.

Department of Pediatrics, Lishui Maternal and Child Health Care Hospital, Lishui, China.

Antimicrobial resistance is a significant clinical problem in pediatric practice in China. Surveillance of antibiotic use is one of the cornerstones to assess the quality of antibiotic use and plan and assess the impact of antibiotic stewardship interventions. We carried out quarterly point prevalence surveys referring to WHO Methodology of Point Prevalence Survey in 16 Chinese general and children's hospitals in 2019 to assess antibiotic use in pediatric inpatients based on the WHO AWaRe metrics and to detect potential problem areas. Data were retrieved via the hospital information systems on the second Monday of March, June, September and December. Antibiotic prescribing patterns were analyzed across and within diagnostic conditions and ward types according to WHO AWaRe metrics and Anatomical Therapeutic Chemical (ATC) Classification. A total of 22,327 hospitalized children were sampled, of which 14,757 (66.1%) were prescribed ≥1 antibiotic. Among the 3,936 sampled neonates (≤1 month), 59.2% ( = 2,331) were prescribed ≥1 antibiotic. A high percentage of combination antibiotic therapy was observed in PICUs (78.5%), pediatric medical wards (68.1%) and surgical wards (65.2%). For hospitalized children prescribed ≥1 antibiotic, the most common diagnosis on admission were lower respiratory tract infections (43.2%, = 6,379). WHO Watch group antibiotics accounted for 70.4% of prescriptions ( = 12,915). The most prescribed antibiotic ATC classes were third-generation cephalosporins (41.9%, = 7,679), followed by penicillins/β-lactamase inhibitors (16.1%, = 2,962), macrolides (12.1%, = 2,214) and carbapenems (7.7%, = 1,331). Based on these data, overuse of broad-spectrum Watch group antibiotics is common in Chinese pediatric inpatients. Specific interventions in the context of the national antimicrobial stewardship framework should aim to reduce the use of Watch antibiotics and routine surveillance of antibiotic use using WHO AWaRe metrics should be implemented.
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http://dx.doi.org/10.3389/fphar.2021.601561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039455PMC
March 2021

Prenatal and postnatal exposure to Bisphenol A and Asthma: a systemic review and meta-analysis.

J Thorac Dis 2021 Mar;13(3):1684-1696

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Background: Bisphenol A (BPA) is a plasticizer with high production and ubiquitous usage in polycarbonate plastics and epoxy resins. The association between prenatal or postnatal exposure to BPA and childhood wheeze/asthma has not been well established. Our study aimed to provide further justification for the current studies.

Methods: Studies were searched from PubMed, Web of Science, Scopus and Embase from inception until Sep 15, 2020. Meta-analysis was performed to calculate pooled adjusted odds ratios (aOR). The methodological quality of included studies was assessed by using the Newcastle Ottawa Scale (NOS).

Results: Of 2,814 screened articles, 9 studies with 3,885 participants were included in the final analysis. When all studies were pooled, postnatal exposure to BPA was associated with a higher risk of childhood asthma (aOR =1.43; 95% CI: 1.28-1.59) or childhood wheeze (aOR =1.38; 95% CI: 1.18-1.62). Prenatal exposure to BPA had a small but significant increased risk of childhood asthma (aOR =1.17; 95% CI: 1.01-1.34). An increased risk of childhood wheeze was related to prenatal exposure to BPA at 16 weeks' gestation (aOR =1.29; 95% CI: 1.07-1.55), but not at 26 weeks' gestation (aOR =1.07; 95% CI: 0.88-1.29) nor at random-time gestation (aOR =1.02; 95% CI: 0.89-1.16).

Conclusions: Prenatal and postnatal exposure to BPA was related to an increased risk of childhood asthma. However, only postnatal and early gestational exposure (at 16 weeks) to BPA could induce the risk of childhood wheeze, but not late gestational exposure (at 26 weeks).
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http://dx.doi.org/10.21037/jtd-20-1550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024800PMC
March 2021

Synergistic anti-tumor effect of anti-PD-L1 antibody cationic microbubbles for delivery of the miR-34a gene combined with ultrasound on cervical carcinoma.

Am J Transl Res 2021 15;13(3):988-1005. Epub 2021 Mar 15.

Medical College of China Three Gorges University Yichang, China.

This study explored the synergistic effect of anti-PD-L1 antibody cationic microbubbles (MBs) for delivery of the miR-34a gene combined with ultrasound in inhibiting the cervical cancer. H&E stain, TUNEL, immunohistochemistry and RT-PCR were used to detect the change of apoptosis regulatory factors, and immunofluorescence, Flow cytometry and LDH assays were applied to evaluate the changing of immunomodulatory. In this experiment the PD-L1 Ab/miR-34a-MBs were prepared successfully. The cell targeting assay showed that U14 cells were surrounded by the PD-L1 Ab/miR-34a-MBs and microbubbles had well contrast imaging capability in vivo. With the irradiation power was 1 W/cm and the irradiation time was 25 s, the gene transfection efficiency was the highest using EGFP plasmid lorded microbubbles. In vivo anti-tumor assays, the PD-L1 Ab/miR-34a-MBs showed a great potential in inhibiting tumor growth with a TGI of >50%. PD-L1 Ab/miR-34a-MBs treatment enhanced the anti-tumor effect compared with that induced by PD-L1 Ab or miR-34a alone. Firstly, PD-L1 Ab/miR-34a-MBs could gather miR-34a with high-concentration aggregation and releasing around the cervical cancer, which takes a significant role in promoting apoptosis by downregulated Bcl-2 and upregulated Bax. Furthermore, combination therapy was found to augment the activation of T lymphocytes proliferation and increase CD8+ T cells infiltration, to enhance antitumor immune killing effect. The anti-PD-L1 antibody microbubbles for delivery miR-34a gene with ultrasound were considered to be a promising combination therapy regimen via initiating apoptotic mechanism of the tumor and anti-tumor immune regulation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014418PMC
March 2021

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway.

Toxins (Basel) 2021 03 12;13(3). Epub 2021 Mar 12.

Key Laboratory of Zoonosis Research, Ministry of Education, Department of Animal Science, College of Animal Sciences, Jilin University, Changchun 130062, China.

Lipopolysaccharide (LPS) is an endotoxin, which may cause immune response and inflammation of bovine mammary glands. Mastitis impairs animal health and results in economic loss. Curcumin (CUR) is a naturally occurring diketone compound, which has attracted widespread attention as a potential anti-inflammatory antioxidant. The purpose of this study is to investigate whether CUR can reduce the damage of bovine mammary epithelial cells (MAC-T) induced by LPS and its underlying molecular mechanism. The MAC-T cell line was treated with different concentrations of LPS and CUR for 24 h. The results showed that CUR rescued the decrease of MAC-T cell viability and cell damage induced by LPS. At the same time, 10 µM CUR and 100 µg/mL LPS were used to treat the cells in the follow-up study. The results showed CUR treatment reduced the accumulation of reactive oxygen species (ROS), the expression of inflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-8 (IL-8), IL-6 and IL-1β) and the rate of apoptosis induced by LPS. These effects were associated with the activation of the nuclear factor E2-related factor 2 (NFE2L2)-antioxidant response element (ARE) pathway coupled with inactivation of the nuclear factor-κB (NF-κB) inflammatory and caspase/Bcl2 apoptotic pathways.
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http://dx.doi.org/10.3390/toxins13030208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999830PMC
March 2021

Lipid metabolism in asthma: Immune regulation and potential therapeutic target.

Cell Immunol 2021 Jun 13;364:104341. Epub 2021 Mar 13.

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China; State Key Lab of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou, China. Electronic address:

Asthma is a chronic inflammatory disease of the lungs that poses a considerable health and socioeconomic burden. Several risk factors work synergistically to affect the progression of asthma. Lipid metabolism, especially in distinct cells such as T cells, macrophages, granulocytes, and non-immune cells, plays an essential role in the pathogenesis of asthma, as lipids are potent signaling molecules that regulate a multitude of cellular response. In this review, we focused on the metabolic pathways of lipid molecules, especially fatty acids and their derivatives, and summarized their roles in various cells during the pathogenesis of asthma along with the current pharmacological agents targeting lipid metabolism.
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http://dx.doi.org/10.1016/j.cellimm.2021.104341DOI Listing
June 2021

Targeting AXL Kinase Sensitizes Leukemic Stem and Progenitor Cells to Venetoclax Treatment in Acute Myeloid Leukemia.

Blood 2021 Mar 30. Epub 2021 Mar 30.

BC Cancer Research Centre, Vancouver, Canada.

The abundance of genetic abnormalities and phenotypic heterogeneities in AML pose significant challenges to developing improved treatments. Here we demonstrated that a key GAS6/AXL axis is highly activated in AML patient cells, particularly in stem/progenitor cells. We developed a potent, selective AXL inhibitor that has favorable pharmaceutical properties and efficacy against preclinical patient-derived xenotransplantation (PDX) models of AML. Importantly, inhibition of AXL sensitized AML stem/progenitor cells to venetoclax treatment, with strong synergistic effects in vitro and in PDX models. Mechanistically, single-cell RNA-sequencing and functional validation studies uncovered that AXL inhibition or in combination with venetoclax potentially targets intrinsic metabolic vulnerabilities of AML stem/progenitor cells, which shows a distinct transcriptomic profile and inhibits mitochondrial oxidative phosphorylation. Inhibition of AXL or BCL-2 also differentially targets key signaling proteins to synergize in leukemic cell killing. These findings have direct translational impact on the treatment of AML and other cancers with high AXL activity.
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http://dx.doi.org/10.1182/blood.2020007651DOI Listing
March 2021

Mitochondrial Oxidative Stress Enhances Vasoconstriction by Altering Calcium Homeostasis in Cerebrovascular Smooth Muscle Cells under Simulated Microgravity.

Biomed Environ Sci 2021 Mar;34(3):203-212

Department of Cardiovascular Medicine, Chinese PLA General Hospital & Chinese PLA Medical School, Beijing 100853, China.

Objective: Exposure to microgravity results in postflight cardiovascular deconditioning in astronauts. Vascular oxidative stress injury and mitochondrial dysfunction have been reported during this process. To elucidate the mechanism for this condition, we investigated whether mitochondrial oxidative stress regulates calcium homeostasis and vasoconstriction in hindlimb unweighted (HU) rat cerebral arteries.

Methods: Three-week HU was used to simulate microgravity in rats. The contractile responses to vasoconstrictors, mitochondrial fission/fusion, Ca distribution, inositol 1,4,5-trisphosphate receptor (IP R) abundance, and the activities of voltage-gated K channels (K ) and Ca -activated K channels (BK ) were examined in rat cerebral vascular smooth muscle cells (VSMCs).

Results: An increase of cytoplasmic Ca and a decrease of mitochondrial/sarcoplasmic reticulum (SR) Ca were observed in HU rat cerebral VSMCs. The abundance of fusion proteins (mitofusin 1/2 [MFN1/2]) and fission proteins (dynamin-related protein 1 [DRP1] and fission-mitochondrial 1 [FIS1]) was significantly downregulated and upregulated, respectively in HU rat cerebral VSMCs. The cerebrovascular contractile responses to vasoconstrictors were enhanced in HU rats compared to control rats, and IP R protein/mRNA levels were significantly upregulated. The current densities and open probabilities of K and BK decreased and increased, respectively. Treatment with the mitochondrial-targeted antioxidant mitoTEMPO attenuated mitochondrial fission by upregulating MFN1/2 and downregulating DRP1/FIS1. It also decreased IP R expression levels and restored the activities of the K and BK channels. MitoTEMPO restored the Ca distribution in VSMCs and attenuated the enhanced vasoconstriction in HU rat cerebral arteries.

Conclusion: The present results suggest that mitochondrial oxidative stress enhances cerebral vasoconstriction by regulating calcium homeostasis during simulated microgravity.
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http://dx.doi.org/10.3967/bes2021.001DOI Listing
March 2021

The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.

Cell Death Differ 2021 Mar 24. Epub 2021 Mar 24.

Department of Anatomy, Histology and Embryology, Health Science Center, Peking University, Beijing, China.

The pathogenesis of Parkinson's disease (PD) remains unclear, and there is no disease-modifying agent for PD. Withaferin A (WA), a naturally occurring compound, has emerged as a neuroprotective agent. However, the mechanisms by which WA is neuroprotective in PD are unknown. Here we show that WA protected against loss of dopaminergic neurons, neuroinflammation, and motor deficits in MPTP-induced PD mouse models. Whole-genome deep sequencing analysis combined with Meta-analysis of human PD studies reveal that DJ1, Nrf2, and STING in substantia nigra pars compacta (SNc) are linked to anti-PD effect of WA. We found that WA activated DJ1 and Nrf2, and suppressed STING within SNc; and overexpression of STING in SNc dampened the effect of WA. Using genetically modified mice (DJ1-KO, Nrf2-KO, STING and STING-KO) and immunolabeling technique, we identified that WA targeted DJ1-Nrf2-STING pathway in dopaminergic neurons; and we demonstrate that STING might be an important factor in PD pathogenesis. In addition, WA alleviated accumulation of phosphorylated α-synuclein (p-α-syn) and insoluble α-syn within SNc in adeno-associated virus (AAV)-mediated human α-syn overexpression PD model. Our comparative analysis on whole-genome transcriptome profiles suggests that STING might be a key target of WA and amantadine in PD treatment. This study highlights a multifaceted role for WA in neuroprotection, and suggests that WA can be a potential candidate for treatment of PD.
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http://dx.doi.org/10.1038/s41418-021-00767-2DOI Listing
March 2021

Prognostic Value of the Glasgow Prognostic Score on Overall Survival in patients with Advanced Non-Small Cell Lung Cancer.

J Cancer 2021 1;12(8):2395-2402. Epub 2021 Mar 1.

Department of Respiratory Oncology, Guangxi Medical University Affiliated Tumor Hospital, No.71 Heti Road, 530021, Nanning City, Guangxi Zhuang Autonomous Region, China.

Findings from previous studies regarding the association between the Glasgow Prognostic Score (GPS) and overall survival (OS) of patients with advanced non-small cell lung cancer (NSCLC) were limited. This study aimed to investigate the prognostic value of GPS in patients with advanced NSCLC after adjusting for potential confounding factors. A retrospective cohort study was conducted in 494 patients with advanced NSCLC between 2009 and 2019. Clinicopathological characteristics (including GPS) were analyzed to determine predictors of OS using univariate and multivariate Cox proportional hazards models. Survival curves were estimated using the Kaplan-Meier method. Of the enrolled patients with advanced NSCLC, 66.46% were men and 53.85% were aged <60 years. The percentages of GPS scores of 0, 1, and 2 were 36.44%, 36.03%, and 27.53%, respectively. The median OS of the GPS 0, 1, and 2 groups were 23.27, 14.37, and 10.27 months, respectively (log-rank P <0.0001). A higher GPS was independently associated with an increased risk of death (P for trend = 0.0004) after full adjustment for potential confounders. The risk of death increased by 77% in the GPS 1 group (hazard ratio [HR]=1.77, 95% confidence interval [CI]=1.22-2.57, P=0.0027) and 109% in the GPS 2 group (HR=2.09, 95%CI=1.36-3.22, P=0.0008) compared with the GPS 0 group after adjustment. We did not find significant heterogeneity among the analyzed subgroups apart from sex (P interaction=0.017). High pretreatment GPS is independently associated with worse OS in patients with advanced NSCLC. GPS should be considered in patient counseling and decision-making and needs to be further validated by large-cohort and prospective studies.
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http://dx.doi.org/10.7150/jca.52215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974889PMC
March 2021

Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts.

Cell Regen 2021 Mar 19;10(1):15. Epub 2021 Mar 19.

Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.

Background: The pluripotent stem cells in planarians, a model for tissue and cellular regeneration, remain further identification. We recently developed a method to enrich piwi-1+ cells in Schmidtea mediterranea, by staining cells with SiR-DNA and Cell Tracker Green, named SirNeoblasts that permits their propagation and subsequent functional study in vivo. Since traditional enrichment for planarian neoblasts by Hoechst 33342 staining generates X1 cells, blocking the cell cycle and inducing cytotoxicity, this method by SiR-DNA and Cell Tracker Green represents a complementary technological advance for functional investigation of cell fate and regeneration. However, the similarities in heterogeneity of cell subtypes between SirNeoblasts and X1 remain unknown.

Results: In this work, we performed single cell RNA sequencing of SirNeoblasts for comparison with differential expression patterns in a publicly available X1 single cell RNA sequencing data. We found first that all of the lineage-specific progenitor cells in X1 were present in comparable proportions in SirNeoblasts. In addition, SirNeoblasts contain an early muscle progenitor that is unreported in X1. Analysis of new markers for putative pluripotent stem cells identified here, with subsequent sub-clustering analysis, revealed earlier lineages of epidermal, muscular, intestinal, and pharyngeal progenitors than have been observed in X1. Using the gcm as a marker, we also identified a cell subpopulation resided in previously identified tgs-1+ neoblasts. Knockdown of gcm impaired the neoblast repopulation, suggesting a function of gcm in neoblasts.

Conclusions: In summary, the use of SirNeoblasts will enable broad experimental advances in regeneration and cell fate specification, given the possibility for propagation and transplantation of recombinant and mutagenized pluripotent stem cells that are not previously afforded to this rapid and versatile model system.
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http://dx.doi.org/10.1186/s13619-021-00076-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979843PMC
March 2021

A 14-gene gemcitabine resistance gene signature is significantly associated with the prognosis of pancreatic cancer patients.

Sci Rep 2021 Mar 17;11(1):6087. Epub 2021 Mar 17.

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

To identify a gemcitabine resistance-associated gene signature for risk stratification and prognosis prediction in pancreatic cancer. Pearson correlation analysis was performed with gemcitabine half maximal inhibitory concentration (IC50) data of 17 primary pancreatic cancer lines from Genomics of Drug Sensitivity in Cancer (GDSC) and the transcriptomic data from GDSC and Broad Institute Cancer Cell Line Encyclopedia, followed by risk stratification, expression evaluation, overall survival (OS) prediction, clinical data validation and nomogram establishment. Our biomarker discovery effort identified a 14-gene signature, most of which featured differential expression. The 14-gene signature was associated with poor OS in E-MTAB-6134 (HR 2.37; 95% CI 1.75-3.2; p < 0.0001), pancreatic cancer-Canada (PACA-CA) (HR 1.76; 95% CI 1.31-2.37; p = 0.00015), and 4 other independent validation cohorts: pancreatic cancer-Australia (PACA-AU) (HR 1.9; 95% CI 1.38-2.61; p < 0.0001), The Cancer Genome Atlas (TCGA) (HR 1.73; 95% CI 1.11-2.69; p = 0.014), GSE85916 (HR 1.97; 95% CI 1.14-3.42; p = 0.014) and GSE62452 (HR 1.82; 95% CI 1.02-3.24; p = 0.039). Multivariate analysis revealed that the 14-gene risk score was an independent pancreatic cancer outcome predictor in E-MTAB-6134 (p < 0.001) and TCGA (p = 0.006). A nomogram including the 14-gene was established for eventual clinical translation. We identified a novel gemcitabine resistance gene signature for risk stratification and robust categorization of pancreatic cancer patients with poor prognosis.
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http://dx.doi.org/10.1038/s41598-021-85680-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969955PMC
March 2021

MicroRNA-483 Functions as an Oncogene in Colorectal Cancer.

Ann Clin Lab Sci 2021 Jan;51(1):30-37

Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi, China

Colorectal cancer is the third leading cancer-related fatal disease in the world, and its morbidity and mortality are increasing. In recent years, the researches of miRNAs have provided new ideas for the diagnosis and treatment of colorectal cancer. Although previous studies have confirmed the abnormal expression of miR-483 in different types of tumors, the expression level of miR-483 in colorectal cancer remains to be elucidated. The effect of up-regulated miR-483 on colorectal cancer cell function was detected by cell function test. In order to further clarify the effect of up-regulation of miR-483 on colorectal cancer cells, we carried out tumorigenesis experiments in nude mice. The results showed that after transplantation of colorectal cancer cells subcutaneously in nude mice, the tumors in the over-expression group of miR-483 were significantly larger than those in the control group. miR-483 is an important regulator of the occurrence and development of colorectal cancer. The results of this study are helpful to understand the development of colorectal cancer and to formulate diagnosis and treatment strategies for colorectal cancer.
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January 2021

[Metabonomics study of Shouhui Tongbian Capsules in slow transit constipation based on UPLC-ESI-QE-Orbitrap-MS].

Zhongguo Zhong Yao Za Zhi 2021 Feb;46(3):532-538

Center for New Drug Pharmacology, Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Linyi 276006 China Linyi Key Laboratory for Immunopharmacology and Immunotoxicology of Natural Medicine Linyi 276006, China.

The effect of Shouhui Tongbian Capsules(SHTB) on the endogenous metabolites of colon tissue in mice with slow transit constipation was analyzed by metabolomics methods to explore its mechanism in the treatment of constipation. ICR mice were randomly divided into normal group, model group and SHTB group according to the body weight. The mice were given diphenoxylate to establish the slow transit constipation model. Mouse carbon ink pushing rate, first defecation time and the number of defecation particles in 12 h were observed. The mouse colon tissue was separated and the mucous cells were detected by Periodic acid Schiff and Alcian blue(AB-PAS) staining. Ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry(UPLC-ESI-Orbitrap-MS/MS) technology was used to characterize the differences in tissue metabolism to screen out the potential different metabolites and possible metabolic pathways in colon tissue. The results indicated that SHTB could significantly shorten the first defecation time and the number of defecations, and increase the number of intestinal peristalsis and mucous cells in the colonic mucosa compared to the model mice. Metabolomics results showed that, compared with the normal group, a total of 17 potential biomarkers, including L-kynurenine, N6,N6,N6-trimethyl-L-lysine, L-formylkynurenine, N6-acetyl-L-lysine, L-phenylalanine, phenylacetaldehyde, xanthoxin, thymidine, glycyl-L-leucine, cystathionine,(R)-1-aminopropan-2-ol, deoxycytidine, gamma-glutamyl-gamma-aminobutyraldehyde, D-galactose, L-arginine, L-proline and pyruvate, were found and identified in colon tissue. Treated with SHTB, these metabolic differences tended to return to normal levels. Therefore, it could be made a conclusion that the therapeutic effect of SHTB on chronic transit constipation may be related to regulating phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, arginine and proline metabolism, cysteine and methionine metabolism, tyrosine metabolism, arginine biosynthesis, pyruvate metabolism, glycolysis, pyrimidine metabolism, tricarboxylic acid cycle and galactose metabolism.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201116.401DOI Listing
February 2021

Fluorinated Polyurethane-Based Enameled Wires with a Low Friction Coefficient.

ACS Omega 2021 Feb 11;6(7):4719-4725. Epub 2021 Feb 11.

School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Fluorinated polyurethane (FPU) with a different fluorine content was prepared using perfluoropolyether glycols, poly(propylene glycol), and isophorone diisocyanate as starting materials, and 1,4-butanediol as a chain extender. The structure and molecular weight of FPU were characterized by Fourier transform infrared spectroscopy and gel permeation chromatography. A solution of FPU in xylene and cresol was then coated on copper wires using an enameled machine to prepare enameled wires. The friction coefficient and adhesion performance of the enameled wires were tested. The friction coefficient of the as-prepared enameled wires reached 0.095, which was much lower than 0.149 of the polyurethane without fluorine. FPU-based enameled wires also showed good mechanical performances and increased breakdown voltages. In addition, FPU exhibited good hydrophobic and oleophobic characterization.
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http://dx.doi.org/10.1021/acsomega.0c05506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905813PMC
February 2021

CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease.

Clin Transl Med 2021 Feb;11(2):e339

School of Medicine, Southeast University, Research Institute of General Surgery, Jinling Hospital, Nanjing, China.

Inflammatory bowel disease (IBD) has emerged a global disease and the ascending incidence and prevalence is accompanied by elevated morbidity, mortality, and substantial healthcare system costs. However, the current typical one-size-fits-all therapeutic approach is suboptimal for a substantial proportion of patients due to the variability in the course of IBD and a considerable number of patients do not have positive response to the clinically approved drugs, so there is still a great, unmet demand for novel alternative therapeutic approaches. Spleen tyrosine kinase (Syk), a cytoplasmic nonreceptor protein tyrosine kinase, plays crucial roles in signal transduction and there are emerging data implicating that Syk participates in pathogenesis of several gut disorders, such as IBD. In this study, we observed the Syk expression in IBD patients and explored the effects of therapeutic Syk inhibition using small-molecule Syk inhibitor piceatannol in bone marrow-derived macrophages (BMDMs). In addition, due to the poor bioavailability and pharmacokinetics of small-molecule tyrosine kinase inhibitors and superiority of targeting nanoparticles-based drug delivery system, we herein prepared piceatannol-encapsulated poly(lactic-co-glycolic acid) nanoparticles that conjugated with chemokine C-C motif ligand 4 (P-NPs-C) and studied its therapeutic effects in vitro in BMDMs and in vivo in experimental colitis model. Our results indicated that in addition to alleviating colitis, oral administration of P-NPs-C promoted the restoration of intestinal barrier function and improved intestinal microflora dysbiosis, which represents a promising treatment for IBD.
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http://dx.doi.org/10.1002/ctm2.339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888545PMC
February 2021

NF-κB pathway play a role in SCD1 deficiency-induced ceramide synthesis.

Cancer Biol Ther 2021 Feb 22;22(2):164-174. Epub 2021 Feb 22.

Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine, Xiamen University, Xiamen, China.

Stearoyl-CoA-desaturase 1 (SCD1) deficiency mediates apoptosis in colorectal cancer cells by promoting ceramide synthesis. The mechanisms underlying the cross-talk between SCD1 and ceramide synthesis have not been explored. We treated colorectal cancer cells with an SCD1 inhibitor and examined the effects on gene expression, cell growth, and cellular lipid contents. The main effect of SCD1 inhibition on the fatty acid contents of colorectal cancer cells was a decrease in monounsaturated fatty acids (MUFAs). RNA sequencing (RNA-seq) showed that the most intense alteration of gene expression after SCD1 inhibition occurred in the NF-κB signaling pathway. Further experiments revealed that SCD1 inhibition resulted in increased levels of phosphorylated NF-κB p65 and increased nuclear translocation of NF-κB p65. Treatment with an NF-κB inhibitor eliminated several effects of SCD1 inhibition, mainly including overexpression of serine palmitoyltransferase1 (SPT1), elevation of dihydroceramide contents, and suppression of cell growth. Furthermore, treatment with supplemental oleate counteracted the SCD1-induced NF-κB activation and downstream effects. In summary, our data demonstrate that the NF-κB pathway plays a role in SCD1 deficiency-induced ceramide synthesis in colorectal cancer cells, and that reduced MUFA levels contribute to the course.
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http://dx.doi.org/10.1080/15384047.2021.1883414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928033PMC
February 2021

Solid-State Lithium Metal Batteries with Extended Cycling Enabled by Dynamic Adaptive Solid-State Interfaces.

Adv Mater 2021 Mar 19;33(12):e2008084. Epub 2021 Feb 19.

Innovation Center for Textile Science and Technology, Donghua University, Shanghai, 200051, China.

Improving the long-term cycling stability of solid-state lithium (Li)-metal batteries (SSBs) is a severe challenge because of the notorious solid-solid interfacial contact loss originating from the repeated expansion and contraction of the Li anodes. Here, it is reported that high-performance SSBs are enabled by constructing brick-and-mortar electrolytes that can dynamically adapt to the interface changes during cycling. An electrolyte film with a high mechanical strain (250%) is fabricated by filling viscoelastic (600% strain) and piezoelectric block-copolymer electrolytes (mortar) into a mixed conductor Li La TiO nanofiber film (brick). During Li-plating, the electrolytes can homogenize the interfacial electric field and generate piezoelectricity to promote uniform Li-deposition, while the mortar can adhere to the Li-anode without interfacial disintegration in the reversed Li-stripping. As a result, the electrolytes show excellent compatibility with the electrodes, leading to a long electrochemical cyclability at room temperature. The symmetrical Li//Li cells run stably for 1880 h without forming dendrites, and the LiFePO /Li full batteries deliver high coulombic efficiency (>99.5%) and capacity retention (>85%) over 550 cycles. More practically, the pouch cells exhibit excellent flexibility and safety for potential practical applications.
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http://dx.doi.org/10.1002/adma.202008084DOI Listing
March 2021

Targeting IL-21 to tumor-reactive T cells enhances memory T cell responses and anti-PD-1 antibody therapy.

Nat Commun 2021 02 11;12(1):951. Epub 2021 Feb 11.

CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

T cell rejuvenation by PD-1/PD-L1 blockade, despite emerging as a highly promising therapy for advanced cancers, is only beneficial for a minority of treated patients. There is evidence that a lack of efficient T cell activation may be responsible for the failure. Here, we demonstrate that IL-21 can be targeted to tumor-reactive T cells by fusion of IL-21 to anti-PD-1 antibody. To our surprise, the fusion protein PD-1Ab21 promotes the generation of memory stem T cells (T) with enhanced cell proliferation. PD-1Ab21 treatment show potent antitumor effects in established tumor-bearing mice accompanied with an increased frequency of T and robust expansion of tumor-specific CD8 T cells with a memory phenotype, and is superior to a combination of PD-1 blockade and IL-21 infusion. Therefore, we have developed a potential strategy to improve the therapeutic effects of immune checkpoint blockade by simultaneously targeting cytokines to tumor-reactive T cells.
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http://dx.doi.org/10.1038/s41467-021-21241-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878483PMC
February 2021

Polydatin Protects Bovine Mammary Epithelial Cells Against Zearalenone-Induced Apoptosis By Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress.

Toxins (Basel) 2021 02 5;13(2). Epub 2021 Feb 5.

Key Laboratory of Zoonosis Research, Department of Animal Science, College of Animal Sciences, Jilin University, Ministry of Education, Changchun 130062, China.

Zearalenone (ZEA) is a mycotoxin of the genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 μM did not affect cell viability. Follow-up studies then applied 30 μM of ZEA and 5 μM of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the gene and up-regulated the gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress.
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http://dx.doi.org/10.3390/toxins13020121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915214PMC
February 2021

The Photomorphogenic Transcription Factor PpHY5 Regulates Anthocyanin Accumulation in Response to UVA and UVB Irradiation.

Front Plant Sci 2020 18;11:603178. Epub 2021 Jan 18.

Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou, China.

Red coloration contributes to fruit quality and is determined by anthocyanin content in peach (). Our previous study illustrated that anthocyanin accumulation is strongly regulated by light, and the effect of induction differs according to light quality. Here we showed that both ultraviolet-A (UVA) and ultraviolet-B (UVB) irradiation promoted anthocyanin biosynthesis in "Hujingmilu" peach fruit, and a combination of UVA and UVB had additional effects. The expression of anthocyanin biosynthesis and light signaling related genes, including transcription factor genes and light signaling elements, were induced following UV irradiation as early as 6 h post-treatment, earlier than apparent change in coloration which occurred at 72 h. To investigate the molecular mechanisms for UVA- and UVB-induced anthocyanin accumulation, the genes encoding ELONGATED HYPOCOTYL 5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), Cryptochrome (CRY), and UV RESISTANCE LOCUS 8 (UVR8) in peach were isolated and characterized through functional complementation in corresponding Arabidopsis () mutants. and restored hypocotyl length and anthocyanin content in Arabidopsis mutants under white light; while and restored expression in Arabidopsis mutants in response to UV irradiation. Arabidopsis transgenic lines accumulated higher amounts of anthocyanin under UV supplementation (compared with weak white light only), especially when UVA and UVB were applied together. These data indicated that PpHY5, acting as AtHY5 counterpart, was a vital regulator in UVA and UVB signaling pathway. In peach, the expression of was up-regulated by UVA and UVB, and PpHY5 positively regulated both its own transcription by interacting with an E-box in its own promoter, and the transcription of the downstream anthocyanin biosynthetic genes (), (), and () as well as the transcription factor gene . In summary, functional evidence supports the role of PpHY5 in UVA and UVB light transduction pathway controlling anthocyanin biosynthesis. In peach this is via up-regulation of expression of genes encoding biosynthetic enzymes, as well as the transcription factor and itself.
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http://dx.doi.org/10.3389/fpls.2020.603178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847898PMC
January 2021

The effects of different temperatures on the storage characteristics of lotus (Nelumbo nucifera G.) root.

Food Chem 2021 Jun 18;348:129109. Epub 2021 Jan 18.

School Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), China. Electronic address:

Lotus root (Nelumbo nucifera G.) is a high economic value crop in the world. In this study, the storage characteristics (color, sensory, texture, and fatty acids) of lotus root ("Elian No.5″) were evaluated at different harvest periods (September 2018, October 2018, November 2018, December 2018, and January 2019). Moreover, the storage characteristics were evaluated after the short- term and long-term storage of lotus root at 4 °C and 20 °C. The hardness of lotus root significantly decreased at both temperatures (4 °C and 20 °C) during the first 3 days of storage. In contrast, the decrease in hardness delayed at 4 °C (beyond 3 days of storage). Further, genes related to hardness at different storage temperatures were identified using the RNA-seq and qRT-PCR. The results of this study provide a reference for lotus root storage and a basis for the molecular breeding of longterm-storable lotus root.
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http://dx.doi.org/10.1016/j.foodchem.2021.129109DOI Listing
June 2021

Expression of transient receptor potential vanilloid genes and proteins in diabetic rat heart.

Mol Biol Rep 2021 Feb 1;48(2):1217-1223. Epub 2021 Feb 1.

Department of Physiology, Medical Science College of China Three Gorges University, Yichang, China.

Cardiac complications are leading causes of death in diabetic patients. Imbalance of Ca homeostasis is a hallmark of cardiac dysfunction in diabetes, while TRPV channels are non-selective for cations and are permeable to Ca. Our aim was to evaluate the expression levels of TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 genes and proteins in cardiac tissue at 3 days and 4, 8, and 12 weeks after induction of diabetes. Sprague-Dawley rats were assigned to control and DM groups. DM was induced by intraperitoneal injection of streptozotocin (60 mg/kg). The expression levels of TRPV genes were analyzed by the quantitative reverse transcription polymerase chain reaction, and TRPV proteins were determined by western blotting. Compared to controls, the expression levels of TRPV2, TRPV3, and TRPV6 in diabetic myocardium did not change, while TRPV1 decreased at 4, 8, and 12 weeks, TRPV4 was upregulated at 3 days and 4, 8, and 12 weeks, TRPV5 mRNA increased at 8 and 12 weeks, and TRPV5 protein increased at 4, 8, and 12 weeks. Our findings showed that TRPV1, TRPV4, and TRPV5 are associated with the diabetic heart.
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http://dx.doi.org/10.1007/s11033-021-06182-7DOI Listing
February 2021

Dynamic Regulation of Lithium Dendrite Growth with Electromechanical Coupling Effect of Soft BaTiO Ceramic Nanofiber Films.

ACS Nano 2021 Feb 26;15(2):3161-3170. Epub 2021 Jan 26.

Innovation Center for Textile Science and Technology, Donghua University, Shanghai 200051, China.

Lithium (Li) metal batteries (LMBs) offer great opportunity for developing high-energy density energy-storage-systems, but the anodes suffer a severe problem of dendrite growth that hinders the practical applications of LMBs. Here, we report a soft BaTiO ceramic nanofiber film with excellent ferroelectricity and piezoelectricity that enables one to transverse the dense deposition of Li metal. During Li plating, the strong ferroelectricity reduces the Li-ion concentration gradient near the anode and thus facilitating their uniform deposition. Once squeezed by these Li deposits, the BaTiO film generates instantaneous piezo-effect to dynamically change the subsequent Li deposition from vertical to lateral. As a result, Li-Cu cells exhibit reversible plating-stripping processes for over 200 cycles with a high Coulombic efficiency of >98.3%. When pairing with high-voltage LiNiCoAlO cathodes, the LMBs can retain >80% capacity in 300 cycles without forming dendrites even under challenging conditions including a high cathode loading of 7.2 mg/cm, a lean electrolyte amount of 7 μL/mg, and high current rates. The findings point to a promising electromechanical coupling strategy to dynamically adjust dendrite growth for designing Li metal anodes.
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http://dx.doi.org/10.1021/acsnano.0c09745DOI Listing
February 2021