Publications by authors named "Yun Yang"

1,053 Publications

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An efficient heterogeneous Ni/NiP catalyst for urea-assisted water electrolysis.

Chem Commun (Camb) 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, P. R. China.

Built-in electrophilic/nucleophilic domains promoted the favorable adsorption of urea molecules on the surface/interface of heterogeneous Ni/NiP for urea oxidation.
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http://dx.doi.org/10.1039/d2cc03566aDOI Listing
August 2022

Analysis of Continuity of Care and Its Related Factors in Diabetic Patients: A Cross-Sectional Study.

Korean J Fam Med 2022 Jul 19;43(4):246-253. Epub 2022 Jul 19.

Department of Family Medicine, Seoul National University Hospital, Seoul, Korea.

Background: Continuity of care in primary care settings is crucial for managing diabetes. We aimed to statistically define and analyze continuity factors associated with demographics, clinical workforce, and geographical relationships.

Methods: We used 2014-2015 National Health Insurance Service claims data from the Korean registry, with 39,096 eligible outpatient attendance. We applied multivariable logistic regression to analyze factors that may affect the continuity of care indices for each patient: the most frequent provider continuity index (MFPCI), modified-modified continuity index (MMCI), and continuity of care index (COCI).

Results: The mean continuity of care indices were 0.90, 0.96, and 0.85 for MFPCI, MMCI and COCI, respectively. Among patient factors, old age >80 years (MFPCI: odds ratio [OR], 0.81; 95% confidence interval [CI], 0.74-0.89; MMCI: OR, 0.84; 95% CI, 0.76-0.92; and COCI: OR, 0.81; 95% CI, 0.74-0.89) and mild disability were strongly associated with lower continuity of care. Another significant factor was the residential area: the farther the patients lived from their primary care clinic, the lower the continuity of diabetes care (MFPCI: OR, 0.74; 95% CI, 0.70-0.78; MMCI: OR, 0.70; 95% CI, 0.66-0.73; and COCI: OR, 0.74; 95% CI, 0.70-0.78).

Conclusion: The geographical proximity of patients' residential areas and clinic locations showed the strongest correlation as a continuity factor. Further efforts are needed to improve continuity of care to address the geographical imbalance in diabetic care.
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http://dx.doi.org/10.4082/kjfm.21.0145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334710PMC
July 2022

Current Status of Physical Activity in South Korea.

Korean J Fam Med 2022 Jul 19;43(4):209-219. Epub 2022 Jul 19.

Department of Family Medicine, Inje University Ilsan Paik Hospital, Goyang, Korea.

The health benefits of physical activity have been well established. However, more than a quarter of the adult population in the world is insufficiently active (not performing at least 150 minutes of moderate-intensity physical activity per week, 75 minutes of vigorous-intensity physical activity per week, or any equivalent combination of the two), and there has been little improvement since 2001. In South Korea, the prevalence of insufficient physical activity was 54.4% in adults and 94.1% in adolescents in 2020. There was continuous decrease in the prevalence of sufficient aerobic physical activity, from 58.3% in 2014 to 45.6% in 2020. However, daily duration of sedentary behavior increased from 7.5 to 8.6 hours in the same period. Interventions to promote physical activity are necessary in various fields including clinical and policy approaches. As physical activity has decreased during the coronavirus disease 2019 pandemic, safer and more effective strategies to promote physical activity are required.
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http://dx.doi.org/10.4082/kjfm.22.0099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334717PMC
July 2022

Clinical Outcomes of the Modified Stoppa Combined with Iliac Fossa Approach for Complex Acetabular Fractures: A Medium- and Long-term Retrospective Study.

Orthop Surg 2022 Jul 25. Epub 2022 Jul 25.

Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, PR China.

Objective: The modified Stoppa combined with iliac fossa approach has gained increasing popularity. Although early clinical outcomes have been satisfactory, extensive long-term clinical outcomes are relatively scarce. The purpose of this study was to evaluate the medium- and long-term outcomes of this approach for complex acetabular fractures.

Methods: This was a retrospective study involving 57 patients with complex acetabular fractures from January 2009 to January 2016. All fractures were treated with the modified Stoppa combined with iliac fossa approach. Follow-up was at least 5 years. Primary outcome measures, including quality of reduction and clinical outcomes, were recorded by an independent observer. Secondary outcome measures included time to surgery, surgical time, intraoperative blood loss, and perioperative complications.

Results: Fifty-seven patients (range, 18-80 years) included 46 males and 11 females. There were 32 cases on the left side and 25 cases on the right side. The most common associated injury was pulmonary contusion. According to the Judet-Letournel classification, there were 27 both-column fractures, 16 anterior column with posterior hemitransverse fractures and 14 T-type fractures. The average time from injury to surgery was 7.3 days. The average intraoperative blood loss and transfusion were 750.9 ml and 564.3 ml, respectively. All fractures healed within 6 months after surgery. The average follow-up time was 7.7 years, and there was no loss of follow-up. The quality of reduction was graded as anatomical in 23 cases (40.4%), imperfect in 22 cases (38.6%), and poor in 12 cases (21.0%). According to grading system of Merle d' Aubigne and Postel, clinical outcomes at 1 year follow-up were excellent in 17 cases (29.8%), good in 25 cases (43.9%), fair in four cases (7.0%), and poor in 11 cases (19.3%). The excellent and good rate was 73.7% and the difference was not statistically significant compared with the clinical outcomes at the last follow-up. Intraoperative complications included four cases of obturator nerve injury and two cases of vascular injury. Postoperative complications included one case of wound delayed healing, two cases of deep vein thrombosis, two cases of avascular necrosis of femoral head, three cases of heterotopic ossification and five cases of post-traumatic arthritis. Only one of these patients underwent reoperation for femoral head necrosis.

Conclusions: The modified Stoppa combined with iliac fossa approach can achieve satisfactory reduction quality and hip function. It might be a valuable alternative to the ilioinguinal approach for the surgical management of acetabular fractures.
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http://dx.doi.org/10.1111/os.13415DOI Listing
July 2022

Single-Atom Catalysts Supported on the Graphene/Graphdiyne Heterostructure for Effective CO Electroreduction.

Inorg Chem 2022 Aug 21;61(30):12012-12022. Epub 2022 Jul 21.

School of Chemical Science and Technology, Yunnan University, Kunming 650091, Yunnan, P. R. China.

Electrochemical reduction of CO to high-energy chemicals is a promising strategy for achieving carbon-neutral energy circulation. However, designing high-performance electrocatalysts for the CO reduction reaction (CORR) remains a great challenge. In this work, by means of density functional theory calculations, we systematically investigate the transition metal (TM) anchored on the nitrogen-doped graphene/graphdiyne heterostructure ([email protected]/GDY) as a single-atom catalyst for CO electroreduction applications. The computational results show that [email protected]/GDY exhibits remarkable activity with a low limiting potential of -0.567 V for the reduction of CO to CH. When the charged [email protected]/GDY system is immersed in a continuum solvent, the reaction barrier decreases to 0.366 eV, which is ascribed to stronger electron transfer between GDY and transition metal atoms in the GRA/GDY heterostructure. In addition, the GRA/GDY heterostructure system significantly weakens the linear scaling relationship between the adsorption free energy of key CO reduction intermediates, which leads to a catalytic activity that is higher than that of the single-GRA system and thus greatly accelerates the CORR. The electronic structure analysis reveals that the appropriate d-π interaction will affect the d orbital electron distribution, which is directly relevant to the selectivity and activity of catalysis. We hope these computational results not only provide a potential electrocatalyst candidate but also open up an avenue for improving the catalytic performance for efficient electrochemical CORR.
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http://dx.doi.org/10.1021/acs.inorgchem.2c02020DOI Listing
August 2022

Cell osteogenic bioactivity mediated precisely by varying scaled micro-pits on ordered micro/nano hierarchical structures of titanium.

Regen Biomater 2022 1;9:rbac046. Epub 2022 Jul 1.

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Hierarchical surface structures with micro-nano scale play a crucial role in regulation of cell proliferation and osteogenic differentiation. It has been proven that cells are extremely sensitive to the nanoscaled structure and show multifarious phenotypes. Though a vital function of microstructure on osseointegration has been confirmed, the cell performances response to different microscaled structure is needed to be further dissected and in depth understood. In this work, the ordered micro-nano hierarchical structures with varying micro-scaled pits were precisely fabricated on titanium successfully by the combination of electrochemical, chemical etching and anodization as well. systematical assessments indicated that the micro-nano multilevel structures on titanium exhibited excellent cells adhesion and spreading ability, as well as steerable proliferation and osteogenic differentiation behaviors. It is shown that smaller micro-pits and lower roughness of the hierarchical structures enabled faster cell propagation. Despite cell growth was delayed on micro-nano titanium with relatively larger cell-match-size micro-pits and roughness, osteogenic-specific genes were significantly elevated. Furthermore, the alkaline phosphatase activity, collagen secretion and extracellular matrix mineralization of MC3T3-E1 on multi-scaled titanium were suppressed by a large margin after adding IWP-2 (an inhibitor of Wnt/β-catenin signal pathway), indicating this pathway played a crucial part in cell osteogenic differentiation modulated by micro-nano structures.
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http://dx.doi.org/10.1093/rb/rbac046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290875PMC
July 2022

New Insights Into Tissue Culture Plant-Regeneration Mechanisms.

Front Plant Sci 2022 30;13:926752. Epub 2022 Jun 30.

Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute, Sichuan Agricultural University, Chengdu, China.

Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one of the most widely used regenerative technologies. Recently, a series of breakthroughs were made in the study of plant regeneration. This review summarizes two regenerative pathways in tissue culture: somatic embryogenesis and organogenesis. Furthermore, we review the environmental factors influencing plant regeneration from explant sources, basal culture medium, plant growth regulators, and light/dark treatment. Additionally, we analyse the molecular mechanisms underlying two pathways. This knowledge will promote an understanding of the fundamental principles of plant regeneration from precursor cells and lay a solid foundation for applying plant micropropagation and genetic modification.
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http://dx.doi.org/10.3389/fpls.2022.926752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280033PMC
June 2022

First-Episode Mania Post-COVID Pneumonitis With No Prior Psychiatric History.

Prim Care Companion CNS Disord 2022 07 7;24(4). Epub 2022 Jul 7.

Northern Area Mental Health Services, The Royal Melbourne Hospital, Epping, Victoria, Australia.

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http://dx.doi.org/10.4088/PCC.22cr03295DOI Listing
July 2022

Irrigation plays significantly different roles in influencing hydrological processes in two breadbasket regions.

Sci Total Environ 2022 Jul 8:157253. Epub 2022 Jul 8.

USDA-ARS Hydrology and Remote Sensing Laboratory, Beltsville, MD 20705-2350, USA.

Agriculture is a major water user, especially in dry and drought-prone areas that rely on irrigation to support agricultural production. In recent years, the over-extraction of groundwater, exacerbated by climate change, population growth, and intensive agricultural irrigation, has led to a drop in water levels and impacts the hydrological cycle. Understanding changes in hydrological processes is essential for pursuing water sustainability. The purpose of this study is to estimate the amount and impact of irrigation on hydrological processes in two breadbasket regions, Jing-Jin-Ji (JJJ), China, and northern Texas (NTX), US. We used the Soil and Water Assessment Tool (SWAT) to explore spatiotemporal variations of irrigation from 2008 to 2013 and compared changes in hydrological processes caused by irrigation. The results indicated that deficit irrigation is more common in JJJ than in NTX and can reduce approximately 50 % of irrigation water use in areas with intensively irrigated cropland. The applied irrigation varies less over time in NTX but fluctuates in JJJ. Compared with NTX, the higher irrigation intensity in JJJ results in a more significant change in downstream peak streamflow of about 6 m/s. Moreover, the difference in crop growing seasons can lead to different impacts of irrigation on hydrological processes. For example, the percentage change of surface runoff under real-world relative to the no-irrigation scenario was the greatest, about 40 %, in JJJ and NTX. However, the peak change happened at different times, with the nearing maturity of winter wheat in May in JJJ and corn in August in NTX. The great potential to reduce groundwater extraction by adopting water conservation irrigation techniques calls for policies and regulations to help farmers shift towards more sustainable water management practices.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157253DOI Listing
July 2022

Identifying the characteristics and potential risk of seawater intrusion for southern China by the SBM-DEA model.

Sci Total Environ 2022 Jul 7:157205. Epub 2022 Jul 7.

Key Laboratory of Surficial Geochemistry of Ministry of Education, School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China.

Seawater intrusion (SWI) seriously affects the economic development of coastal areas in southern China, and understanding its mechanisms is the basis for effective control of SWI. Hydrogeochemical methods and slack-based measurement data envelopment analysis (SBM-DEA) are used to study the characteristics and potential risk of SWI in coastal cities of southern China. Types and distribution of SWI, coastal groundwater evolution, geological-geographic and economic threatens of SWI, potential SWI risk, and environmental management recommendations are explored. The results show that the intrusion areas of Zhejiang and Guangdong account for 94.1 % of the total intrusion area of southern China, and the intrusion degree in Zhejiang is the highest, followed by Guangdong and Fujian. SWI is prone to occur on the sandy and silty coasts of the plain area of southern China; it accelerates the groundwater evolution speed and shortens the evolution path. SBM-DEA can be well applied to evaluate the potential risk of SWI events, and the results indicate a noticeable difference in the environmental performance level of coastal cities in southern China. The low environmental performance level (<0.3) and severe SWI of Taizhou and Zhanjiang indicate that SWI gradually worsens with economic development. In contrast, the high environmental performance level (>0.7) and low SWI of Wenzhou, Fuzhou, Quanzhou, Shantou, and Beihai indicate that the potential risk of SWI is gradually decreasing. Moreover, this study confirms that the environmental Kuznets curve (EKC) phenomenon exists in SWI events for southern China, and SWI-EKC indicates that the urban development of south China is approaching maturity. The specific case of SWI and EPL in coastal cities of south China jointly indicates that optimizing industrial structure, implementing a resources management policy, and improving citizens' environmental awareness are fundamental measures to resolve the contradiction between economic development and environmental problems.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157205DOI Listing
July 2022

The Dynamic Change in Fatty Acids during the Postharvest Process of Oolong Tea Production.

Molecules 2022 Jul 4;27(13). Epub 2022 Jul 4.

Key Laboratory of Tea Science in Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

As important factors to oolong tea quality, the accumulation and dynamic change in aroma substances attracts great attention. The volatile composition of oolong tea is closely related to the precursor contents. Fatty acids (FAs) and their derivatives are basic components of oolong tea fragrance during the postharvest process. However, information about the precursors of FAs during the postharvest process of oolong tea production is rare. To investigate the transformation of fatty acids during the process of oolong tea production, gas chromatograph-flame ionization detection (GC-FID) was conducted to analyze the composition of FAs. The results show that the content of total polyunsaturated FAs initially increased and then decreased. Specifically, the contents of α-linolenic acid, linoleic acid and other representative substances decreased after the turn-over process of oolong tea production. The results of partial least squares discrimination analysis (PLS-DA) showed that five types of FAs were obviously impacted by the processing methods of oolong tea (VIP > 1.0). LOX (Lipoxygenase, EC 1.13.11.12) is considered one of the key rate-limiting enzymes of long-chain unsaturated FAs in the LOX-HPL (hydroperoxide lyase) pathway, and the mechanical wounding occurring during the postharvest process of oolong tea production greatly elevated the activity of LOX.
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http://dx.doi.org/10.3390/molecules27134298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268070PMC
July 2022

Four-year follow-up of LCAR-B38M in relapsed or refractory multiple myeloma: a phase 1, single-arm, open-label, multicenter study in China (LEGEND-2).

J Hematol Oncol 2022 07 6;15(1):86. Epub 2022 Jul 6.

Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 West 5th Road, Xi'an, 710004, China.

Background: LCAR-B38M is a chimeric antigen receptor T cell product with two binding domains targeting B cell maturation antigen. Our previous reports showed a remarkable efficacy of LCAR-B38M in patients with relapsed/refractory multiple myeloma (RRMM) at a median follow-up of 2 years. Here, we report long-term safety and efficacy data from a median follow-up of 4 years.

Methods: LEGEND-2 was a phase 1, single-arm, open-label study conducted in four registered sites in China. Seventy-four participants with RRMM received LCAR-B38M treatment. Lymphodepletion was performed using cyclophosphamide or cyclophosphamide plus fludarabine. LCAR-B38M, at a median dose of 0.513 × 10 cells/kg, was intravenously administered either in three split infusions or in a single infusion. The primary objective was the safety of LCAR-B38M, and the secondary objective was efficacy.

Results: As of May 25, 2021, the median follow-up was 47.8 months. All patients experienced ≥ 1 adverse events (AEs). Grade ≥ 3 AEs were observed in 45/74 (60.8%) patients. Cytokine release syndrome (CRS) occurred in 68/74 (91.9%) cases; 7 (9.5%) had grade ≥ 3 CRS. One patient experienced grade 1 central nervous system toxicity. The overall response rate was 87.8%. Fifty-four out of 74 (73.0%) patients achieved complete response. The median progression-free survival was 18.0 months, and the median overall survival for all patients was not reached. The median duration of response was 23.3 months. Four patients experienced viral infection more than 6 months post-infusion, and four patients developed second primary non-hematological malignancies at a median time of 11.5 months post-CAR-T cell transfer.

Conclusions: The 4-year follow-up data of LCAR-B38M therapy demonstrated a favorable long-term safety profile and a durable response in patients with RRMM. Trial registration Clinicaltrials.gov NCT03090659 (retrospectively registered on March 27, 2017); ChiCTR-ONH-17012285.
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http://dx.doi.org/10.1186/s13045-022-01301-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261106PMC
July 2022

Bio-inspired Superwettable Surface for the Detection of Cancer Biomarker: A Mini Review.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221110670

Advanced Materials Institute, 12689Qilu University of Technology (Shandong Academy of Sciences), Jinan, People's Republic of China.

Inspired by nature, superwettable material-based biosensors have aroused wide interests due to their potential in cancer biomarker detection. This mini review mainly summarized the superwettable materials as novel biosensing substrates for the development of evaporation-induced enrichment-based signal amplification and visual biosensing method. Biosensing applications based on the superhydrophobic surfaces, superwettable micropatterned surfaces, and slippery lubricant-infused porous surfaces for various cancer biomarker detections were described in detail. Finally, an insight of remaining challenges and perspectives of superwettable biosensor is proposed.
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http://dx.doi.org/10.1177/15330338221110670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340408PMC
July 2022

Pervasive translation of circular RNAs driven by short IRES-like elements.

Nat Commun 2022 06 29;13(1):3751. Epub 2022 Jun 29.

Bio-med Big Data Center, CAS Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Nutrition and Health, Shanghai, China.

Some circular RNAs (circRNAs) were found to be translated through IRES-driven mechanism, however the scope and functions of circRNA translation are unclear because endogenous IRESs are rare. To determine the prevalence and mechanism of circRNA translation, we develop a cell-based system to screen random sequences and identify 97 overrepresented hexamers that drive cap-independent circRNA translation. These IRES-like short elements are significantly enriched in endogenous circRNAs and sufficient to drive circRNA translation. We further identify multiple trans-acting factors that bind these IRES-like elements to initiate translation. Using mass-spectrometry data, hundreds of circRNA-coded peptides are identified, most of which have low abundance due to rapid degradation. As judged by mass-spectrometry, 50% of translatable endogenous circRNAs undergo rolling circle translation, several of which are experimentally validated. Consistently, mutations of the IRES-like element in one circRNA reduce its translation. Collectively, our findings suggest a pervasive translation of circRNAs, providing profound implications in translation control.
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http://dx.doi.org/10.1038/s41467-022-31327-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242994PMC
June 2022

Simple, rapid, and visual electrochemiluminescence sensor for on-site catechol analysis.

RSC Adv 2022 Jun 13;12(27):17330-17336. Epub 2022 Jun 13.

Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Department of Pharmacy, School of Medicine, Hunan Normal University Changsha 410013 Hunan China.

Environmental pollution caused by aromatic compounds such as catechol (Cat) has become a major issue for human health. However, there is no simple, rapid, and low-cost method for on-site monitoring of Cat. Here, based on ECL quenching mechanism, we develop a simple, rapid and visual mesoporous silica (MSNs)-electrochemiluminescence (ECL) sensor for on-site monitoring of Cat. The mechanism of ECL quenching is due to the interaction between Cat and Ru(bpy) and the interactions between the oxidation products of Cat and DBAE. MSNs films with ordered perpendicular mesopore channels exhibit an amplification effect of ECL intensity due to the negatively charged pore channel. There is a good linear relationship between ECL intensity and Cat concentration in the range of 10 ∼ 1000 μM with the limit of detection (LOD) of 9.518 μM ( = 0.99). The on-site sensor is promising to offer new opportunities for pharmaceuticals analysis, on-site monitoring, and exposure risk assessment.
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http://dx.doi.org/10.1039/d2ra03067eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189704PMC
June 2022

Cadinane-type sesquiterpenoid dimeric diastereomers hibisceusones A-C from infected stems of Hibiscus tiliaceus with cytotoxic activity against triple-negative breast cancer cells.

Bioorg Chem 2022 Jun 23;127:105982. Epub 2022 Jun 23.

Hainan Branch Institute of Medicinal Plant Development (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China; Institute of Medicinal Plant Development (Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Chinese Academy of Medical Sciences), Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China. Electronic address:

Three new cadinane-type sesquiterpenoid dimeric diastereomers (1-3) named hibisceusones A-C were obtained from the infected stems of Hibiscus tiliaceus. The structures were determined by NMR spectroscopy and MS techniques, and the absolute configurations were assigned by ECD and single-crystal X-ray diffraction techniques. Compounds 1-3 are diastereomers, and contain a 1,4-dioxane ring linearly fused to different cadinane-type polycyclic skeletons. This is the first time that such a structure has been identified in natural products. Compounds 1-3 exhibited cytotoxic activities, and 2 showed a significantly high anti-triple-negative breast cancer (TNBC) effect. The anti-cancer effect of compound 2 was 3-4 fold higher than that of 1 and 3. The anti-cancer effect was generated via the induction of the apoptosis of the MDA-MB-231 cells by inhibiting the PI3Kα pathway.
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http://dx.doi.org/10.1016/j.bioorg.2022.105982DOI Listing
June 2022

Safety and immunogenicity of a hybrid-type vaccine booster in BBIBP-CorV recipients in a randomized phase 2 trial.

Nat Commun 2022 06 27;13(1):3654. Epub 2022 Jun 27.

The Sixth Laboratory, National Vaccine and Serum Institute (NVSI), Beijing, China.

NVSI-06-08 is a potential broad-spectrum recombinant COVID-19 vaccine that integrates the antigens from multiple SARS-CoV-2 strains into a single immunogen. Here, we evaluate the safety and immunogenicity of NVSI-06-08 as a heterologous booster dose in BBIBP-CorV recipients in a randomized, double-blind, controlled, phase 2 trial conducted in the United Arab Emirates (NCT05069129). Three groups of healthy adults over 18 years of age (600 participants per group) who have administered two doses of BBIBP-CorV 4-6-month, 7-9-month and >9-month earlier, respectively, are randomized 1:1 to receive either a homologous booster of BBIBP-CorV or a heterologous booster of NVSI-06-08. The incidence of adverse reactions is low, and the overall safety profile is quite similar between two booster regimens. Both Neutralizing and IgG antibodies elicited by NVSI-06-08 booster are significantly higher than those by BBIBP-CorV booster against not only SARS-CoV-2 prototype strain but also multiple variants of concerns (VOCs). Especially, the neutralizing antibody GMT against Omicron variant induced by heterologous NVSI-06-08 booster reaches 367.67, which is substantially greater than that boosted by BBIBP-CorV (GMT: 45.03). In summary, NVSI-06-08 is safe and immunogenic as a booster dose following two doses of BBIBP-CorV, which is immunogenically superior to the homologous boost with another dose of BBIBP-CorV.
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http://dx.doi.org/10.1038/s41467-022-31379-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237056PMC
June 2022

Tumor-targeted/reduction-triggered composite multifunctional nanoparticles for breast cancer chemo-photothermal combinational therapy.

Acta Pharm Sin B 2022 Jun 10;12(6):2710-2730. Epub 2021 Sep 10.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu 610041, China.

Breast cancer has become the most commonly diagnosed cancer type in the world. A combination of chemotherapy and photothermal therapy (PTT) has emerged as a promising strategy for breast cancer therapy. However, the intricacy of precise delivery and the ability to initiate drug release in specific tumor sites remains a challenging puzzle. Therefore, to ensure that the therapeutic agents are synchronously delivered to the tumor site for their synergistic effect, a multifunctional nanoparticle system (PCRHNs) is developed, which is grafted onto the prussian blue nanoparticles (PB NPs) by reduction-responsive camptothecin (CPT) prodrug copolymer, and then modified with tumor-targeting peptide cyclo(Asp-d-Phe-Lys-Arg-Gly) (cRGD) and hyaluronic acid (HA). PCRHNs exhibited nano-sized structure with good monodispersity, high load efficiency of CPT, triggered CPT release in response to reduction environment, and excellent photothermal conversion under laser irradiation. Furthermore, PCRHNs can act as a photoacoustic imaging contrast agent-guided PTT. studies indicate that PCRHNs exhibited excellent biocompatibility, prolonged blood circulation, enhanced tumor accumulation, allow tumor-specific chemo-photothermal therapy to achieve synergistic antitumor effects with reduced systemic toxicity. Moreover, hyperthermia-induced upregulation of heat shock protein 70 in the tumor cells could be inhibited by CPT. Collectively, PCRHNs may be a promising therapeutic way for breast cancer therapy.
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http://dx.doi.org/10.1016/j.apsb.2021.08.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214336PMC
June 2022

Pathogen-sugar interactions revealed by universal saturation transfer analysis.

Science 2022 07 22;377(6604):eabm3125. Epub 2022 Jul 22.

Sir William Dunn School of Pathology, Oxford, UK.

Many pathogens exploit host cell-surface glycans. However, precise analyses of glycan ligands binding with heavily modified pathogen proteins can be confounded by overlapping sugar signals and/or compounded with known experimental constraints. Universal saturation transfer analysis (uSTA) builds on existing nuclear magnetic resonance spectroscopy to provide an automated workflow for quantitating protein-ligand interactions. uSTA reveals that early-pandemic, B-origin-lineage severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike trimer binds sialoside sugars in an "end-on" manner. uSTA-guided modeling and a high-resolution cryo-electron microscopy structure implicate the spike N-terminal domain (NTD) and confirm end-on binding. This finding rationalizes the effect of NTD mutations that abolish sugar binding in SARS-CoV-2 variants of concern. Together with genetic variance analyses in early pandemic patient cohorts, this binding implicates a sialylated polylactosamine motif found on tetraantennary N-linked glycoproteins deep in the human lung as potentially relevant to virulence and/or zoonosis.
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http://dx.doi.org/10.1126/science.abm3125DOI Listing
July 2022

Effects of turning over intensity on fatty acid metabolites in postharvest leaves of Tieguanyin oolong tea ().

PeerJ 2022 13;10:e13453. Epub 2022 Jun 13.

College of Horticulture/Key Laboratory of Tea Science in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China.

Fatty acid derived volatiles (FADVs) are major contributors to the aroma quality of oolong tea (). Most of the processing time for oolong tea is taken up by turning over treatments, but the full profile of fatty acid metabolic changes during this process remains unclear. In this study, we detected fatty acids, their derived volatiles, and related genes of Tieguanyin oolong tea using biochemical and molecular biology methods. The results showed that with an increase in turning over intensities, the content of total unsaturated fatty acids continuously dropped and the content of characteristic FADVs, such as hexanoic acid (Z)-3-Hexenly ester and 2-exenal, continued to increase. Lipoxygenase (LOX), a key gene family in the fatty acid metabolic pathway, showed different patterns, and (TEA025499.1) was considered to be a key gene during the turning over processes. We found that fruit-like aroma (Z)-3-Hexen-1-ol acetate had a strong correlation with the expression levels of eight family genes. Tieguanyin had relatively rich pleasant volatile compounds with moderate turning over intensity (five times turning over treatments). This study provides an overall view of how fatty acid metabolites change and affect the quality of oolong tea with different turning over intensities during processing.
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http://dx.doi.org/10.7717/peerj.13453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202550PMC
June 2022

Genetic relationship and source species identification of 58 Qi-Nan germplasms of Aquilaria species in China that easily form agarwood.

PLoS One 2022 16;17(6):e0270167. Epub 2022 Jun 16.

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China.

Recently, Qi-Nan germplasm, the germplasm of Aquilaria species that easily forms agarwood, has been widely cultivated in Guangdong and Hainan Provinces in China. Since the morphological characteristics of Qi-Nan germplasm are similar to those of Aquilaria species and germplasm is bred by grafting, it is difficult to determine the source species of this germplasm by traditional taxonomic characteristics. In this study, we performed a DNA barcoding analysis of 58 major Qi-Nan germplasms as well as Aquilaria sinensis, A. yunnanensis, A. crassna, A. malaccensis and A. hirta with 5 primers (nuclear gene internal transcribed spacer 2 (ITS2) and the chloroplast genes matK, trnH-psbA, rbcL and trnL-trnF). This field survey in the Qi-Nan germplasm plantations in Guangdong and Hainan Provinces aimed to accurately identify the source species of Qi-Nan germplasm. According to the results, ITS2 and matK showed the most variability and the highest divergence at all genetic distances. This ITS2+matK combination, screened for with TaxonDNA analysis, showed the highest success rate in species identification of the Qi-Nan germplasm. Clustering in the phylogenetic trees constructed with Bayesian inference and maximum likelihood indicated that the Qi-Nan germplasm was most closely related to A. sinensis and more distantly related to A. yunnanensis, A. crassna, A. malaccensis and A. hirta. Therefore, this study determined that the source species of the Qi-Nan germplasm is A. sinensis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270167PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202955PMC
June 2022

Computerized-Assisted Scoliosis Diagnosis Based on Faster R-CNN and ResNet for the Classification of Spine X-Ray Images.

Comput Math Methods Med 2022 6;2022:3796202. Epub 2022 Jun 6.

Department of Medical Examination Center, Huaqiao University Affiliated Strait Hospital, Quanzhou, Fujian 362000, China.

In order to reduce the subjectivity of preoperative diagnosis and achieve accurate and rapid classification of idiopathic scoliosis and thereby improving the standardization and automation of spinal surgery diagnosis, we implement the Faster R-CNN and ResNet to classify patient spine images. In this paper, the images are based on spine X-ray imaging obtained by our radiology department. We compared the results with the orthopedic surgeon's measurement results for verification and analysis and finally presented the grading results for performance evaluation. The final experimental results can meet the clinical needs, and a fast and robust deep learning-based scoliosis diagnosis algorithm for scoliosis can be achieved without manual intervention using the X-ray scans. This can give rise to a computerized-assisted scoliosis diagnosis based on X-ray imaging, which has strong potential in clinical utility applied to the field of orthopedics.
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http://dx.doi.org/10.1155/2022/3796202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192275PMC
June 2022

Anatomical relationship between the omohyoid muscle and the internal jugular vein on ultrasound guidance.

BMC Anesthesiol 2022 06 13;22(1):181. Epub 2022 Jun 13.

Department of Anesthesiology, the First People's Hospital of Huzhou, the Affiliated Hospital of Huzhou Teachers College, Guangchanghou Road 158th, 313000, Huzhou, People's Republic of China.

Background: Internal jugular vein catheterization is widely used in clinical practice, and there are many related studies on internal jugular vein catheterization. However, the omohyoid muscle, which is adjacent to the internal jugular vein, is a rarely mentioned muscle of the infrahyoid muscles group. The purpose of this study is to explore the anatomical relationship between the omohyoid muscle and the internal jugular vein on ultrasound guidance and provide a theoretical reference for jugular puncture and catheterization.

Methods: The study included 30 volunteers. The volunteer's head lay in the neutral position and was then turned to the left at an angle of 30°, 45° and 60° with the bed surface, as verified using an adjustable protractor. A high-frequency ultrasound probe (6-14 Hz) was used to examine the plane of the apex of sternocleidomastoid triangle (PAST), the triangle consists of anatomical landmarks: a base was clavicle, its sides - heads of sternocleidomastoid muscle. And the plane of the middle of sternocleidomastoid triangle(PMST) which was a horizontal line, connecting midpoints of both sides. The right omohyoid muscle (OM) and the right internal jugular vein (IJV) were observed and recorded for statistical analysis.

Results: There were statistically significant differences in the number of overlapping cases of OM and IJV at each head rotation angle between the PAST and PMST groups. There were statistically significant differences between the angles which OM and IJV centre point line and the left horizontal position of the PAST and PMST at different body angles.

Conclusion: The traditional middle route puncture point is the apex of the sternocleidomastoid triangle, which can effectively avoid injury to the omohyoid muscle, to an extent.

Trail Registration: ChiCTR2000034233 , Registered 29/06/2020. www. Chinese Clinical Trial Registry.gov.
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http://dx.doi.org/10.1186/s12871-022-01723-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9190082PMC
June 2022

The inhibition of protein translation promotes tumor angiogenic switch.

Mol Biomed 2022 Jun 13;3(1):18. Epub 2022 Jun 13.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, No.17 Renmin South Road Section Three, Chengdu, 610041, Sichuan, China.

The 'angiogenic switch' is critical for tumor progression. However, the pathological details and molecular mechanisms remain incompletely characterized. In this study, we established mammal xenografts in zebrafish to visually investigate the first vessel growth (angiogenic switch) in real-time, by inoculating tumor cells into the perivitelline space of live optically transparent Transgenic (flk1:EGFP) zebrafish larvae. Using this model, we found that hypoxia and hypoxia-inducible factor (HIF) signaling were unnecessary for the angiogenic switch, whereas vascular endothelial growth factor A gene (Vegfa) played a crucial role. Mechanistically, transcriptome analysis showed that the angiogenic switch was characterized by inhibition of translation, but not hypoxia. Phosphorylation of eukaryotic translation initiation factor 2 alpha (Eif2α) and the expression of Vegfa were increased in the angiogenic switch microtumors, and 3D tumor spheroids, and puromycin-treated tumor cells. Vegfa overexpression promoted early onset of the angiogenic switch, whereas Vegfa knockout prevented the first tumor vessel from sprouting. Pretreatment of tumor cells with puromycin promoted the angiogenic switch in vivo similarly to Vegfa overexpression, whereas Vegfa knockdown suppressed the increase. This study provides direc and dynamic in vivo evidences that inhibition of translation, but not hypoxia or HIF signaling promotes the angiogenic switch in tumor by increasing Vegfa transcription.
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http://dx.doi.org/10.1186/s43556-022-00081-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192909PMC
June 2022

Impact of pathological response after preoperative transcatheter arterial chemoembolization (TACE) on incidences of microvascular invasion and early tumor recurrence in hepatocellular carcinoma: a multicenter propensity score matching analysis.

Hepatobiliary Surg Nutr 2022 Jun;11(3):386-399

The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.

Background: To study the influence of pathological responses (PR) after transcatheter arterial chemoembolization (TACE) on incidences of microvascular invasion (MVI) and early recurrence in hepatocellular carcinoma (HCC) patients.

Methods: Between 2013 to 2015, consecutive HCC patients who underwent liver resection with "curative" intent at three hospitals were enrolled in this study. Patients with different areas of PR after preoperative TACE were compared with those without preoperative TACE on the incidences of MVI, early recurrence rates and patterns of recurrence before and after propensity score matching (PSM).

Results: Of 1,970 patients, 737 patients who received preoperative TACE were divided into three groups according to the areas of PR: ≥90% (n=226), 60-90% (n=447), and <60% (n=64). PR ≥90% was an independent protective factor of incidences of MVI [odds ratio (OR), 0.144; 95% confidence interval (CI), 0.082-0.245, P<0.001) and early recurrence (HR, 0.742; 95% CI, 0.561-0.963, P=0.032); while PR<60% was an independent risk factor of incidences of MVI (OR, 6.076; 95% CI, 3.004-11.728, P<0.001) and early recurrence (HR, 1.428; 95% CI, 1.095-1.929; P=0.009). Furthermore, patients with PR <60% were significantly more likely to develop multiple intrahepatic recurrences involving multiple hepatic segments when compared with patients without preoperative TACE.

Conclusions: This study indicated the area of PR after TACE was closely associated with the incidences of MVI and early tumor recurrence. Patients with PR <60% were at significantly higher risks of having more MVI, early and multiple tumor recurrences.
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http://dx.doi.org/10.21037/hbsn-20-700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186194PMC
June 2022

Lipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes.

PLoS Pathog 2022 06 9;18(6):e1010552. Epub 2022 Jun 9.

Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing, China.

Arboviruses are etiological agents of various severe human diseases that place a tremendous burden on global public health and the economy; compounding this issue is the fact that effective prophylactics and therapeutics are lacking for most arboviruses. Herein, we identified 2 bacterial lipases secreted by a Chromobacterium bacterium isolated from Aedes aegypti midgut, Chromobacterium antiviral effector-1 (CbAE-1) and CbAE-2, with broad-spectrum virucidal activity against mosquito-borne viruses, such as dengue virus (DENV), Zika virus (ZIKV), Japanese encephalitis virus (JEV), yellow fever virus (YFV) and Sindbis virus (SINV). The CbAEs potently blocked viral infection in the extracellular milieu through their lipase activity. Mechanistic studies showed that this lipase activity directly disrupted the viral envelope structure, thus inactivating infectivity. A mutation in the lipase motif of CbAE-1 fully abrogated the virucidal ability. Furthermore, CbAEs also exert lipase-dependent entomopathogenic activity in mosquitoes. The anti-arboviral and entomopathogenic properties of CbAEs render them potential candidates for the development of novel transmission control strategies against vector-borne diseases.
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http://dx.doi.org/10.1371/journal.ppat.1010552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182268PMC
June 2022

Solvent polishing engineering for quasi-two-dimensional perovskite blue light-emitting diodes.

Chem Commun (Camb) 2022 Jun 23;58(51):7132-7135. Epub 2022 Jun 23.

College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325027, China.

Solvent polishing engineering is adopted to remove the relatively loose defect layer without damaging carrier injection in blue perovskite light-emitting-diodes (PeLEDs). Synchronously, the polishing effects depending on the solvents are discussed in detail. Finally, optimized blue PeLEDs were obtained with a maximum external quantum efficiency of 5.18%, high brightness of 3737 cd m, and a low turn-on voltage of 3.2 V.
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http://dx.doi.org/10.1039/d2cc02268kDOI Listing
June 2022

Immunogenicity and safety of NVSI-06-07 as a heterologous booster after priming with BBIBP-CorV: a phase 2 trial.

Signal Transduct Target Ther 2022 06 6;7(1):172. Epub 2022 Jun 6.

Lanzhou Institute of Biological Products Company Limited, Lanzhou, China.

The increased coronavirus disease 2019 (COVID-19) breakthrough cases pose the need of booster vaccination. We conducted a randomised, double-blinded, controlled, phase 2 trial to assess the immunogenicity and safety of the heterologous prime-boost vaccination with an inactivated COVID-19 vaccine (BBIBP-CorV) followed by a recombinant protein-based vaccine (NVSI-06-07), using homologous boost with BBIBP-CorV as control. Three groups of healthy adults (600 individuals per group) who had completed two-dose BBIBP-CorV vaccinations 1-3 months, 4-6 months and ≥6 months earlier, respectively, were randomly assigned in a 1:1 ratio to receive either NVSI-06-07 or BBIBP-CorV boost. Immunogenicity assays showed that in NVSI-06-07 groups, neutralizing antibody geometric mean titers (GMTs) against the prototype SARS-CoV-2 increased by 21.01-63.85 folds on day 28 after vaccination, whereas only 4.20-16.78 folds of increases were observed in control groups. For Omicron variant, the neutralizing antibody GMT elicited by homologous boost was 37.91 on day 14, however, a significantly higher neutralizing GMT of 292.53 was induced by heterologous booster. Similar results were obtained for other SARS-CoV-2 variants of concerns (VOCs), including Alpha, Beta and Delta. Both heterologous and homologous boosters have a good safety profile. Local and systemic adverse reactions were absent, mild or moderate in most participants, and the overall safety was quite similar between two booster schemes. Our findings indicated that NVSI-06-07 is safe and immunogenic as a heterologous booster in BBIBP-CorV recipients and was immunogenically superior to the homologous booster against not only SARS-CoV-2 prototype strain but also VOCs, including Omicron.
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http://dx.doi.org/10.1038/s41392-022-00984-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167817PMC
June 2022

Model-Based Computational Analysis on the Effectiveness of Enhanced Recovery after Surgery in the Operating Room with Nursing.

Front Surg 2022 18;9:922684. Epub 2022 May 18.

The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Objective: In order to better understand the relative surgical process, this work used a model-based computational analysis on the effectiveness of enhanced recovery after surgery (ERAS) in the operating room with nursing.

Methods: A total of 360 surgical patients in the First Affiliated Hospital, Sun Yat-sen University, from the period June 2020 to March 2021, were randomly divided into two groups, namely, observation group and control group, with 180 cases in each group. Routine nursing was used in the control group, while ERAS was implemented in the observation group from the point of view of four aspects, namely, preoperative visit, intraoperative cooperation, postoperative return visit, and psychological intervention.

Results: Postoperative complications, average hospital stay, nursing satisfaction, and postoperative quality of life in the observation group were significantly better than those in the control group (all < 0.05).

Conclusion: The application of ERAS for surgical patients can enhance team awareness, optimize the process of cooperation, reduce surgical complications and improve nursing quality, and prognosis, and it is worth popularizing in the operating room.
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http://dx.doi.org/10.3389/fsurg.2022.922684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159805PMC
May 2022

An electrochemical aptasensor based on intelligent walking DNA nanomachine with cascade signal amplification powered by nuclease for Mucin 1 assay.

Anal Chim Acta 2022 Jun 21;1214:339964. Epub 2022 May 21.

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, Yunnan, 650500, PR China. Electronic address:

Intelligently walking DNA nanomachines have sprung up an upsurge in various nucleic acid testing, but the rapid and sensitive test methods toward disease biomarker proteins based on the signal amplification strategy of DNA nanomachines were still ongoing development. In this work, an electrochemical aptasensor coupling the magnetic separation technique with the nuclease-powered walking DNA nanomachine was established for Mucin 1 (MUC1) detection. The magnetic beads (MBs) were modified by MUC1 aptamer hybridized with blocker DNA probe (BDP). After reacting with MUC1 proteins, the BDP was released from MBs to trigger the opening of capture hairpin DNA on Au nanoparticle (Au NPs)/MXene-modified electrode surface. In the presence of exonuclease III (Exo III), the BDP as a DNA walker is activated to autonomously move on the electrode. Then, lots of residual DNA fragments can still stay on electrode, further hybridizing with hairpin DNA, which can capture more UiO-66-NH metal-organic frameworks (MOFs). The amounts of ligands in MOFs can generate enhanced differential pulse voltammetry (DPV) signal probes. Furthermore, the concentrations of MUC1 can convert into the amplified DPV signals by introducing the signal amplification between the BDP as DNA walkers and Exo III as driven forces. This proposed electrochemical aptasensor achieved MUC1 detection ranging from 5 pg/mL to 50 ng/mL with detection limit of 0.72 pg/mL. Consequently, the designed and nuclease-powered walking DNA nanomachine provided an efficient strategy for the quantitative analysis of proteins by the interconversion between protein and BDP as a walker, which exhibited practical applicability of MUC1 detection in human serum.
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http://dx.doi.org/10.1016/j.aca.2022.339964DOI Listing
June 2022
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