Publications by authors named "Yun Xue"

126 Publications

Lung stem cells in regeneration and tumorigenesis.

J Genet Genomics 2021 Mar 17. Epub 2021 Mar 17.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Pulmonary Hospital, Tongji University, Shanghai 200092, China. Electronic address:

Adult lung is a highly quiescent organ, with extremely low cell turnover frequency. However, emerging evidences support the occurrence of repair and regeneration in pulmonary epithelia in response to various injuries. Lung regeneration mainly depends on the proliferation of regionally distributed pulmonary stem cells that re-enter the cell cycle. Genetic lineage-tracing approaches help to track the lung epithelial differentiation and/or de-differentiation path, and single-cell transcriptomic technique reveals the essential molecular signaling involved in lung regeneration. Dysregulation of the molecular signaling that balances quiescence and self-renewal leads to the transformation of lung stem cells, and thus promotes lung cancer development. Interestingly, different subtypes of lung cancer share common cells of origin and the pathological transition among various subtypes is responsible for drug resistance in the clinic. In this review, we summarize the recent understanding of lung stem cells in regeneration and tumorigenesis as well as related molecular mechanisms, with the hope to provide helpful insights for clinical treatments of respiratory diseases.
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http://dx.doi.org/10.1016/j.jgg.2020.12.004DOI Listing
March 2021

Simple and green synthesis of calcium alginate/AgCl nanocomposites with low-smoke flame-retardant and antimicrobial properties.

Cellulose (Lond) 2021 Mar 22:1-17. Epub 2021 Mar 22.

College of Chemistry and Chemical Engineering, Shandong Collaborative Innovation Center of Marine Biobased Fibers and Ecological Textiles, Qingdao University, Qingdao, 266071 China.

Fire hazards and infectious diseases result in great threats to public safety and human health, thus developing multi-functional materials to deal with these issues is critical and yet has remained challenging to date. In this work, we report a facile and eco-friendly synthetic approach for the preparation of calcium alginate/silver chloride (CA/AgCl) nanocomposites with dual functions of excellent flame-retardant and antibacterial activity. Multi characterization techniques and antibacterial assays were performed to investigate the flame-retardant and antibacterial properties of the CA/AgCl nanocomposites. The obtained results show that the CA/AgCl nanocomposites exhibited much higher limiting oxygen index value (> 60%) than that of CA (48%) with a UL-94 rating of V-0. Moreover, CA/AgCl particularly displayed an efficiently smoke-suppressive feature by achieving a total smoke release value of 2.7 m/m, which was reduced by 91%, compared to CA. The antibacterial rates of the CA/AgCl nanocomposites against E. coli and S. aureus were measured to be 99.67% and 99.77%, respectively, while CA showed quite weak antibacterial rates. In addition, the flame-retardant and antibacterial mechanisms were analyzed and proposed based on the experimental data. This study provides a novel nanocomposite material with both flame-retardant and antibacterial properties which show promising application prospects in the fields of decorative materials and textile industry.
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http://dx.doi.org/10.1007/s10570-021-03825-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982765PMC
March 2021

Production, characterization, and bioactivities of exopolysaccharides from the submerged culture of M. H. Liu.

3 Biotech 2021 Mar 26;11(3):145. Epub 2021 Feb 26.

School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013 China.

In this study, the submerged culture conditions for exopolysaccharide (EPS) production by M. H. Liu were screened and optimized, and the physicochemical and biological properties of EPS were investigated. Results showed that the glucose and tryptone were the best C and N sources for the maximum EPS production, respectively. Under the optimal culture conditions, the EPS production achieved 1.60 g/L at day 6 in a 5 L stirred tank reactor. Two purified fractions (i.e., Fr-I and Fr-II) were obtained from the EPS by gel permeation chromatography. Fr-II had a higher yield (87.54%), carbohydrate (95.86%), and protein (3.07%) contents and lower molecular weight (74.56 kDa) than that of Fr-I. Both Fr-I and Fr-II were polysaccharide-protein complexes with different monosaccharide compositions and chemical structures. Fr-II also exhibited stronger radical scavenging abilities, antioxidant capacities, and α-amylase and α-glycosidase inhibitory activities in vitro than that of Fr-I. Therefore, Fr-II obtained from . EPS might be explored as potential natural functional components or supplements for applications in food, medicine, and cosmetics.
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http://dx.doi.org/10.1007/s13205-021-02696-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910359PMC
March 2021

Resveratrol Confers Vascular Protection by Suppressing TLR4/Syk/NLRP3 Signaling in Oxidized Low-Density Lipoprotein-Activated Platelets.

Oxid Med Cell Longev 2021 25;2021:8819231. Epub 2021 Feb 25.

MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, China.

This study investigated the effect of resveratrol on Toll-like receptor 4- (TLR4-) mediated matrix metalloproteinase 3 (MMP3) and MMP9 expression in oxidized low-density lipoprotein- (ox-LDL-) activated platelets and the potential molecule mechanism. Human platelets were used in the present study. The results showed that resveratrol suppressed TLR4, MMP3, and MMP9 expression in ox-LDL-activated platelets. The TLR4 inhibitor CLI-095 also inhibited MMP3 and MMP9 expression and secretion in ox-LDL- and lipopolysaccharide- (LPS-) activated platelets. The combination of resveratrol and CLI-095 synergistically suppressed MMP3 and MMP9 expression in ox-LDL- and LPS-activated platelets. These findings suggest that the resveratrol-induced inhibition of MMP3 and MMP9 expression is linked to the suppression of TLR4 activation. Resveratrol also suppressed spleen tyrosine kinase (Syk) phosphorylation and nucleotide-binding domain leucine-rich repeat containing protein 3 (NLRP3) expression and IL-1 secretion in ox-LDL- and LPS-treated platelets. The coimmunoprecipitation results showed that resveratrol inhibited the binding of Syk and NLRP3. Finally, resveratrol reduced vascular senescence cells and the expression of TLR4, MMP3, and MMP9 and prevented alterations of vascular structure in 52-week-old mice. Our findings demonstrated that resveratrol decreased inflammatory protein expression and improved vascular structure in aged mice. Resveratrol inhibited the expression of TLR4 and secretion of MMP3, MMP9, and IL-1. The mechanism of action of resveratrol appears to be associated with the inhibition of TLR4/Syk/NLRP3 activation in ox-LDL-activated platelets.
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http://dx.doi.org/10.1155/2021/8819231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935581PMC
February 2021

LncRNA XIST Promotes Atherosclerosis by Regulating miR-599/TLR4 Axis.

Inflammation 2021 Feb 10. Epub 2021 Feb 10.

Department of Endocrinology, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, No. 68, Gehu Road, Changzhou, 213161, China.

Long noncoding RNAs (lncRNAs) have been reported to be implicated in various biological and pathological processes. However, the function and mechanism of XIST in vascular smooth muscle cells (VSMCs) remains unknown. The levels of XIST, miR-599, and TLR4 were tested by RT-qPCR. VSMCs and human mononuclear cells (U937) treated with ox-LDL were used as atherosclerosis (AS) cell models. The CCK-8 assay was adopted to detect cell viability. Cell apoptosis was examined by the TUNEL assay. A dual-luciferase reporter assay was employed to investigate the interaction between miR-599 and XIST or TLR4. In this research, we uncovered that the XIST level was elevated in the serum of AS patients and ox-LDL-treated AS cell models. Functional analysis revealed that XIST depletion restrained cell proliferation, while induced the apoptosis in AS cell models. Besides, miR-599 was verified to be a direct downstream target of XIST and miR-599 inhibitor reversed the effects of XIST knockdown on AS progression. Finally, we demonstrated that XIST increased TLR4 expression by serving as a ceRNA of miR-599. All these findings manifested the role of the XIST/miR-599/TLR4 axis in AS development.
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http://dx.doi.org/10.1007/s10753-020-01391-xDOI Listing
February 2021

Nanog maintains stemness of Lkb1-deficient lung adenocarcinoma and prevents gastric differentiation.

EMBO Mol Med 2021 Mar 13;13(3):e12627. Epub 2021 Jan 13.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.

Growing evidence supports that LKB1-deficient KRAS-driven lung tumors represent a unique therapeutic challenge, displaying strong cancer plasticity that promotes lineage conversion and drug resistance. Here we find that murine lung tumors from the Kras ; Lkb1 (KL) model show strong plasticity, which associates with up-regulation of stem cell pluripotency genes such as Nanog. Deletion of Nanog in KL model initiates a gastric differentiation program and promotes mucinous lung tumor growth. We find that NANOG is not expressed at a meaningful level in human lung adenocarcinoma (ADC), as well as in human lung invasive mucinous adenocarcinoma (IMA). Gastric differentiation involves activation of Notch signaling, and perturbation of Notch pathway by the γ-secretase inhibitor LY-411575 remarkably impairs mucinous tumor formation. In contrast to non-mucinous tumors, mucinous tumors are resistant to phenformin treatment. Such therapeutic resistance could be overcome through combined treatments with LY-411575 and phenformin. Overall, we uncover a previously unappreciated plasticity of LKB1-deficient tumors and identify the Nanog-Notch axis in regulating gastric differentiation, which holds important therapeutic implication for the treatment of mucinous lung cancer.
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http://dx.doi.org/10.15252/emmm.202012627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933951PMC
March 2021

Synthesis of TEMPO radical decorated hollow porous aromatic frameworks for selective oxidation of alcohols.

Chem Commun (Camb) 2021 Jan;57(7):907-910

School of Chemistry and Materials Science, Key Lab for Functional Materials Chemistry of Guizhou Province, Guizhou Normal University, 116 Baoshan Road North, Guiyang 550001, P. R. China and NanoFCM INC., Xiamen Pioneering Park for Overseas Chinese Scholars, Xiamen, 361005, P. R. China.

A bottom-up approach was developed to prepare TEMPO radical decorated hollow aromatic frameworks (HPAF-TEMPO) by using TEMPO radical functionalized monomers and SiO2 nanospheres as templates. The accessible inner layer, high density of TEMPO sites, and hybrid micro-/mesopores of the HPAF-TEMPO enable the aerobic oxidation of a broad range of alcohols with high efficiency and excellent selectivity.
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http://dx.doi.org/10.1039/d0cc06965eDOI Listing
January 2021

Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease.

Front Aging Neurosci 2020 19;12:512097. Epub 2020 Nov 19.

Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, China.

There is a significant gender difference in the incidence and symptoms of Alzheimer's disease (AD), but its mechanisms are not completely understood. Recent studies showed that NLRP1 inflammasome was overexpressed in females under some pathological conditions such as nodular melanoma. Whether NLRP1 signals have a gender difference in AD has not been elucidated. This study was designed to investigate gender difference on the expressions of NLRP1 signals including NLRP1, Capase-1 and IL-1β in the brains of APP/PS1 mice. Female and male APP/PS1 mice (30-weeks-old) were used in this study. Amyloid-β (Aβ) plaques were stained with Congo red dye and cell apoptosis was detected by TUNEL staining. Expressions of NLRP1, Capase-1 and IL-1β were measured by immunofluorescent staining and Western blotting assay. The numbers of Aβ plaques in cortex and hippocampus and neuronal apoptosis in cortex were 4 and 2-folds in females than males, respectively ( < 0.001). The average size of Aβ plaques in both cortex (females: 3527.11 ± 539.88 μm vs. males: 1920.44 ± 638.49 μm) and hippocampus (females: 1931 ± 308.61 μm vs. males: 1038.55 ± 220.40 μm) were also larger in females than males ( < 0.01). More interestingly, expressions of NLRP1, Caspase-1, and IL-1β were markedly increased in the cortex of females as compared with males. These findings show that NLRP1 signals are higher in brains of female APP/PS1 mice than males, which may be related to the gender differences of AD.
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http://dx.doi.org/10.3389/fnagi.2020.512097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710982PMC
November 2020

A combination of plasma exchange and steroids in the treatment of a-lipoic acid-induced insulin autoimmune syndrome.

Endokrynol Pol 2021 9;72(1):81-82. Epub 2020 Dec 9.

Department of Endocrinology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.

Not required for Clinical Vignette.
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http://dx.doi.org/10.5603/EP.a2020.0085DOI Listing
December 2020

Assembly of two novel coordination polymers by selecting ditopic or chelating auxiliary ligands with naphthalene-2,6-dicarboxylic acid: synthesis, structure and luminescence sensing.

Acta Crystallogr C Struct Chem 2020 12 24;76(Pt 12):1076-1084. Epub 2020 Nov 24.

School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei, Anhui 230601, People's Republic of China.

The Fe ion as a ubiquitous metal plays a key role in biochemical processes. Iron deficiency or excess in the human body can induce various diseases. Thus, effective detection of the Fe ion has been deemed an issue of focus. To develop more crystalline chemical sensors for the selective detection of Fe, two novel two-dimensional (2D) coordination polymers, namely, poly[[[μ-bis(pyridin-4-yl)amine-κN:N'](μ-naphthalene-2,6-dicarboxylato-κO:O)zinc(II)] 0.5-hydrate], {[Zn(CHO)(CHN)]·0.5HO}, 1, and poly[(4,4'-dimethyl-2,2'-bipyridine-κN,N')(μ-naphthalene-2,6-dicarboxylato-κO:O)hemi(μ-naphthalene-2,6-dicarboxylic acid-κO:O)copper(II)] [Cu(CHO)(CHN)(CHO)], 2, have been prepared using solvothermal methods. Single-crystal X-ray diffraction analysis shows that compound 1 is an undulating twofold interpenetrated 2D (4,4)-sql network and compound 2 is a twofold interpenetrated 2D honeycomb-type network with a (6,3)-hcb topology. In addition, 1 exhibits highly selective sensing for the Fe ion.
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http://dx.doi.org/10.1107/S2053229620015260DOI Listing
December 2020

Mutation of G-protein γ subunit DEP1 increases planting density and resistance to sheath blight disease in rice.

Plant Biotechnol J 2021 03 1;19(3):418-420. Epub 2020 Dec 1.

College of Plant Protection, Shenyang Agricultural University, Shenyang, China.

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http://dx.doi.org/10.1111/pbi.13500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955884PMC
March 2021

The case of a painful blue thumb.

JAAD Case Rep 2020 Oct 31;6(10):1053-1055. Epub 2020 Jul 31.

Upstate Dermatology, Greer, South Carolina.

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http://dx.doi.org/10.1016/j.jdcr.2020.06.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509360PMC
October 2020

In Vitro Generation of Red Blood Cells from Stem Cell and Targeted Therapy.

Cell Transplant 2020 Jan-Dec;29:963689720946658

College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, China.

Red blood cell (RBC) transfusion is a common therapeutic intervention, which is necessary for patients with emergency or hematological disorders to reduce morbidity and mortality. However, to date, blood available for transfusion is a limited resource, and the transfusion coverage system still depends on the volunteer-based collection system. The scarcity of blood supplies commonly develops because of local conditions that transiently affect collection. Moreover, donor-derived infectious disease transmission events also remain a risk. Thus, there is a huge demand for artificial blood. The production of cultured RBCs from stem cells is slowly emerging as a potential alternative to donor-derived red cell transfusion products. In this concise review, we summarize the recent in vitro expansion of RBCs from various stem cell sources, targeted therapy, prospects, and remaining challenges.
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http://dx.doi.org/10.1177/0963689720946658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563022PMC
August 2020

Molecular pathogenesis of the hyaluronic acid capsule of Pasteurella multocida.

Microb Pathog 2020 Dec 6;149:104380. Epub 2020 Jul 6.

Laboratory of Veterinary Biologics Engineering, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471023, China; Key-Disciplines Lab of Safety of Environment and Animal Product, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471023, China. Electronic address:

Pasteurella multocida possesses a viscous capsule polysaccharide on the cell surface, which is a critical structural component and virulence factor. Capsular polysaccharides are structurally similar to vertebrate glycosaminoglycans, providing an immunological mechanism for bacterial molecular mimicry, resistance to phagocytosis, and immune evasion during the infection process. Based on the capsular antigen, P. multocida is divided into A, B, D, E, and F five serogroups. Previously, we systematically reported the biosynthesis and regulation mechanisms of the P. multocida capsule. In this paper, we take serogroup A capsular polysaccharide as the representative, systematically illuminating the P. multocida capsular virulence and epidemiology, molecular camouflage, adhesion and colonization, anti-phagocytosis, anti-complement system, cell invasion and signal transduction mechanism, to provide a theoretical basis for the research of molecular pathogenic mechanism of P. multocida capsule and the development of polysaccharides vaccine.
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http://dx.doi.org/10.1016/j.micpath.2020.104380DOI Listing
December 2020

Correction to: LncRNA NONRATT021972 involved the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia after myocardial ischemic injury.

Purinergic Signal 2020 Sep;16(3):463-465

Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.

In the original article, Figs. 2, 3b, and 4b were published incorrectly.
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http://dx.doi.org/10.1007/s11302-020-09712-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524901PMC
September 2020

Specific gut microbiome signature predicts the early-stage lung cancer.

Gut Microbes 2020 07 2;11(4):1030-1042. Epub 2020 Apr 2.

State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences , Shanghai, China.

Alterations of gut microbiota have been implicated in multiple diseases including cancer. However, the gut microbiota spectrum in lung cancer remains largely unknown. Here we profiled the gut microbiota composition in a discovery cohort containing 42 early-stage lung cancer patients and 65 healthy individuals through the 16S ribosomal RNA (rRNA) gene sequencing analysis. We found that lung cancer patients displayed a significant shift of microbiota composition in contrast to the healthy populations. To identify the optimal microbiota signature for noninvasive diagnosis purpose, we took advantage of Support-Vector Machine (SVM) and found that the predictive model with 13 operational taxonomic unit (OTU)-based biomarkers achieved a high accuracy in lung cancer prediction (area under curve, AUC = 97.6%). This signature performed reasonably well in the validation cohort (AUC = 76.4%), which contained 34 lung cancer patients and 40 healthy individuals. To facilitate potential clinical practice, we further constructed a 'patient discrimination index' (PDI), which largely retained the prediction efficiency in both the discovery cohort (AUC = 92.4%) and the validation cohort (AUC = 67.7%). Together, our study uncovered the microbiota spectrum of lung cancer patients and established the specific gut microbial signature for the potential prediction of the early-stage lung cancer.
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http://dx.doi.org/10.1080/19490976.2020.1737487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524275PMC
July 2020

A Case of Nivolumab-Induced Cutaneous Toxicity with Multiple Morphologies.

Dermatopathology (Basel) 2019 Oct-Dec;6(4):255-259. Epub 2020 Jan 31.

Department of Dermatology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Cutaneous reactions are among the most prevalent immune-related adverse events in patients treated with immunotherapy. Given that immunotherapies often act through blocking inhibitory signals on T cells, these treatments also have the potential to generate a host of immune toxicities. We report the case of a 73-year-old woman with a history of non-small cell lung cancer treated with nivolumab 10 months prior to presentation who developed painful nodules, bullae, and a scaly rash on her extremities. Four months after discontinuation of nivolumab, she noted an acute eruption of painful nodules on her extremities, followed by pink papules and tense bullae on her palms and soles. Biopsies were performed of three lesions in sites of varying morphologies. These findings were felt to be consistent with a nivolumab-induced lichenoid reaction. She was initially treated with intralesional steroid injections, topical steroid ointment, and liquid nitrogen cryotherapy with minimal improvement. As the lesions continued to progress, the patient was admitted to the hospital and started on intravenous methylprednisolone. She eventually transitioned to daily oral prednisone with a slow taper with good effect and no recurrence of lesions.
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http://dx.doi.org/10.1159/000505353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098352PMC
January 2020

A novel three-dimensional zinc(II) coordination polymer based on 3,3'-{[1,3-phenylenebis(methylene)]bis(oxy)}dibenzoic acid and 1,4-bis(pyridin-4-yl)benzene: synthesis, crystal structure and photocatalytic properties.

Acta Crystallogr C Struct Chem 2020 Apr 31;76(Pt 4):353-358. Epub 2020 Mar 31.

School of Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224051, People's Republic of China.

A novel three-dimensional (3D) Zn coordination polymer, namely, poly[[[1,4-bis(pyridin-4-yl)benzene](μ-3,3'-{[1,3-phenylenebis(methylene)]bis(oxy)}dibenzoato)zinc(II)] 1,4-bis(pyridin-4-yl)benzene], {[Zn(CHO)(CHN)]·CHN} or {[Zn(PMBD)(DPB)]·DPB}, 1, where HPMBD is 3,3'-{[1,3-phenylenebis(methylene)]bis(oxy)}dibenzoic acid and DPB is 1,4-bis(pyridin-4-yl)benzene, has been synthesized by self-assembly using zinc nitrate, a semi-rigid dicarboxylic acid and a nitrogen-containing ligand. The single-crystal X-ray structure determination indicates that 1 possesses an intriguing 3D architecture with a 4-connected uninodal cds topology, which is constructed from dinuclear {Zn} clusters and V-shaped PMBD linkers. Compound 1 exhibits excellent photocatalytic activity on the degradation of the organic dyes Rhodamine B (RhB), Rhodamine 6G (Rh6G) and Methyl Red (MR).
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http://dx.doi.org/10.1107/S2053229620004052DOI Listing
April 2020

Tailoring the molecular structure of crosslinked polymers for pervaporation desalination.

Nat Commun 2020 03 19;11(1):1461. Epub 2020 Mar 19.

College of Materials Science and Engineering, Beijing University of Chemical Technology, 100029, Beijing, China.

Polymer crosslinking imbues chemical stability to thin films at the expense of lower molecular transportation rates. Here in this work we deployed molecular dynamics simulations to optimise the selection of crosslinking compounds that overcome this trade-off relationship. We validated these simulations using a series of experiments and exploited this finding to underpin the development of a pervaporation (PV) desalination thin-film composite membrane with water fluxes reaching 234.9 ± 8.1 kg m h and salt rejection of 99.7 ± 0.2 %, outperforming existing membranes for pervaporation and membrane distillation. Key to achieving this state-of-the-art desalination performance is the spray coating of 0.73 μm thick crosslinked dense, hydrophilic polymers on to electrospun nanofiber mats. The desalination performances of our polymer nanocomposites are harnessed here in this work to produce freshwater from brackish water, seawater and brine solutions, addressing the key environmental issue of freshwater scarcity.
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http://dx.doi.org/10.1038/s41467-020-15038-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081321PMC
March 2020

A 67-Year-Old Male with Diffuse Purpuric Vesicles and Bullae.

Dermatopathology (Basel) 2019 Oct-Dec;6(4):251-254. Epub 2020 Jan 24.

Department of Dermatology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Leukocytoclastic vasculitis (LCV) is a small-vessel vasculitis that most commonly affects the postcapillary venules in the skin. It classically presents with purpuric macules that progress to palpable purpura on the bilateral shins 7-10 days after an inciting medication or infection, or in the setting of connective tissue disease, malignancy, or inflammatory bowel disease. Up to 50% of cases have no identifiable cause. Lesions on the buttocks, abdomen, upper extremities, and face are uncommon, as are bullae and ulcers. We present a rare case of bullous LCV manifesting as grouped vesicles on the face and body mimicking varicella-zoster infection.
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http://dx.doi.org/10.1159/000503662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011730PMC
January 2020

The prevalence of amphenicol resistance in Escherichia coli isolated from pigs in mainland China from 2000 to 2018: A systematic review and meta-analysis.

PLoS One 2020 11;15(2):e0228388. Epub 2020 Feb 11.

Lab of Medical Microbiology Engineering, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, China.

Background: Amphenicols have been widely used in the pig industry in China, leading to varying degrees of drug resistance.

Methods: The systematic review was performed according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analysis) recommendations on studies investigating the prevalence of amphenicol-resistant Escherichia coli (E. coli) isolated from pig in mainland China from 2000 to 2018, a random-effects model was selected, then followed by meta-analysis.

Results: A total of 103 articles were included in the study. The results of the meta-analysis revealed that the pooled summarized prevalence of resistance to chloramphenicol (CAP) was 72.31% (95% confidence interval (95% CI) = 67.12%-77.23%) and to florfenicol (FF) was 58.64% (95% CI = 52.48%-64.67%). During the past 18 years, the resistance rate to CAP remained high initially but then declined rapidly after 2012, whereas the resistance rate to FF plateaued (54.13%-59.60%) from 2000-2018. In different parts of China, the rate of resistance to amphenicols among E. coli isolates was fairly consistent, with the exception of the north and northwest regions.

Conclusions: In 2002, the veterinary use of CAP was prohibited and its resistance levels in E. coli isolated from pigs was initially maintained at a high level but then showed an obvious downward trend in recent years. Resistance to commonly used FF remained at a high but stable level.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228388PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012437PMC
April 2020

Cephalosporin Resistance in Isolated from Children with Septicemia in Mainland China from 2007 to 2017: A Systematic Review and Meta-Analysis.

Microb Drug Resist 2020 Oct 3;26(10):1250-1259. Epub 2020 Feb 3.

Laboratory of Medical Microbiology Engineering, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, China.

Septicemia in children in mainland China has recently become a public health concern. A meta-analysis was performed on studies investigating the prevalence of cephalosporin-resistant isolated from children with septicemia in mainland China from 2007 to 2017 following a search of relevant databases. A total of 43 articles reporting 11 cephalosporins were included in the review. The results of the meta-analysis revealed that for the first-generation cephalosporins, the pooled summarized prevalence of resistance to cefazolin was 74.96% (95% confidence interval [CI]: 64.79-83.91) and cephalothin resistance was 62.28% (95% CI: 36.45-100). Regarding the second-generation cephalosporins, cefoxitin-resistant comprised 23.85% (95% CI: 10.60-40.40) and cefuroxime resistance was 60.32% (95% CI: 51.25-68.73). For the third-generation cephalosporins, the pooled summarized prevalence of resistance was 51.34% for cefotaxime (95% CI: 40.08-62.54), 40.43% for ceftazidime (95% CI: 31.07-50.15), 45.51% for cefoperazone (95% CI: 20.41-70.61), 12.10% for cefoperazone/sulbactam (95% CI: 6.55-18.76), 62.99% for ceftriaxone (95% CI: 55.00-70.98), and 0% for cefotetan. Among the fourth-generation cephalosporins, resistance to cefepime was 34.08% (95% CI: 25.91-43.31). Most third-generation cephalosporins (, cefotaxime and ceftriaxone) retained high resistance rates throughout the 11-year study period without significant changes. The new fourth-generation cephalosporin, cefepime, is rapidly gaining resistance. Interestingly, ceftazidime, cefepime, and cefoperazone/sulbactam showed a recent decreasing trend of drug resistance. These situations may present a risk for treating children with septicemia and should be closely monitored and treated.
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http://dx.doi.org/10.1089/mdr.2018.0368DOI Listing
October 2020

A Green, Rapid and Efficient Dual-Sensors for Highly Selective and Sensitive Detection of Cation (Hg) and Anion (S) Ions Based on CMS/AgNPs Composites.

Polymers (Basel) 2020 Jan 5;12(1). Epub 2020 Jan 5.

College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.

Detection of mercury (Hg) and sulfide (S), universal and well-known toxic ions, is crucial in monitoring several diseases. How to design and fabricate the high-performance sensor for simultaneously and accurately detecting the Hg and S is critical. Herein, we proposed a novel and convenient strategy for optical detection of Hg and S by employing a carboxymethyl cellulose sodium/silver nanoparticle (CMS/AgNPs) colloidal solution, in which AgNPs were used as monitor for Hg and S, and the CMS was utilized as both the stabilizer and the hydrophilic substrate for AgNPs. Well-identifiable peaks for Hg and S were obtained in water based on UV-VIS absorption spectra, the absorbance intensity and/or position of nano-silver vary with the addition of Hg cation and S anion, accompanying with color change. Impressively, the optimal AgNPs anchored CMS exhibited a high sensitivity and selectivity toward Hg and S, the change in absorbance was linear with the concentration of Hg (0-50 μM) and S (15-70 μM), and the lowest limits of detection (LOD) were 1.8 × 10 M and 2.4 × 10 M, respectively. More importantly, owing to the superior properties in testing Hg and S, the fabricated sensor was successfully applied for detection of target ions in lake and tap water samples. All these good results implied that the designed strategy and as-designed samples is promising in detecting cation (Hg) and anion (S) ions and open up new opportunities for selecting other kinds of ions.
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http://dx.doi.org/10.3390/polym12010113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023171PMC
January 2020

Facile and Green Fabrication of Carrageenan-Silver Nanoparticles for Colorimetric Determination of Cu and S.

Nanomaterials (Basel) 2020 Jan 1;10(1). Epub 2020 Jan 1.

State Key Laboratory of Bio-Fibers and Eco-Textiles, Shandong Collaborative Innovation Center of Marine Biobased Fibers and Ecological Textiles, Institute of Marine Biobased Materials, Qingdao University, Qingdao 266071, China.

In the present work, silver nanoparticles (AgNPs) were prepared by a simple and green method using carrageenan as reducing and capping agent. The as-synthesized carrageenan-AgNPs was demonstrated as an effective duel colorimetric sensing for selective and sensitive recognition of Cu and S, which could be used to detect these ions with naked eyes. In addition, the possible sensing mechanism was that Cu ions caused serious aggregation of carrageenan-AgNPs, which led to the color change of carrageenan-AgNPs. AgNPs were etched by S forming AgS, which played an important role in the determination of S ions. Furthermore, it has been successfully applied to the determination of Cu and S in tap water and lake water, showing its great potential for the analysis of environmental water samples.
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http://dx.doi.org/10.3390/nano10010083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023203PMC
January 2020

Selective Extraction of Light Lanthanides(III) by ,-Di(2-ethylhexyl)-diglycolamic Acid: A Comparative Study with ,-Dimethyl-diglycolamic Acid as a Chelator in Aqueous Solutions.

ACS Omega 2019 Dec 27;4(24):20797-20806. Epub 2019 Nov 27.

College of Nuclear Science and Technology, Harbin Engineering University, Harbin, Heilongjiang 150001, China.

The complexation and selectivity of ,-di(2-ethylhexyl)-diglycolamic acid (HDEHDGA/kerosene, HA) toward the light lanthanides, La(III), Ce(III), Pr(III), and Nd(III), are presented for the extraction from chloride media. In the low pH region (pH 1.8-2.8), the obtained data reveal that the extraction of Ln(III) is governed by cation-exchange mechanism and is driven by the negative change in enthalpy. The results from the slope analysis method suggest the formation of LnA·(HA) in the extraction process. As major extracted species with a core of LnA in the first coordination sphere, LnA might connect with one or two additional HA molecules in the second coordination sphere by hydrogen bonding. The LnA core might share similar coordination geometry to those of 1:3 Ln(III) complexes (LnA') with water-soluble ,-dimethyl-diglycolamic acid (HDMDGA, HA') formed in aqueous solutions or in solid-state compounds. The correlation between the extraction with HDEHDGA (HA) as an extractant and the complexation with HDMDGA (HA') as a chelator has been explored by interpreting the separation factors for HA with the difference in the stability constants for HA'. Consequently, the ratios of the stability constants of the corresponding 1:3 complexes (LnA') with HDMDGA could be reasonably translated to the separation factors (SFs) with HDEHDGA, providing a valuable approach for understanding the origin of the extraction/separation mechanism. By comparing the extraction selectivity of HDEHDGA with that of the currently deployed extractants in the industry such as P204, P507, and Cyanex 272, HDEHDGA offers outstanding selectivity with considerable SFs (SF = 6.68, SF = 2.79, and SF = 2.65) for light Ln(III) pairs under conditions of low acid concentrations.
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http://dx.doi.org/10.1021/acsomega.9b03241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906934PMC
December 2019

Paravertebral Block with Compound Betamethasone in Laparoscopic Cholecystectomy: A Double-blind Randomized Controlled Trial.

Cureus 2019 Oct 29;11(10):e6023. Epub 2019 Oct 29.

Anesthesiology, Fuling Central Hospital of Chongqing City, Chongqing, CHN.

Backgrounds The aim of this study was to evaluate the utility of unilateral single injection thoracic paravertebral block (TPVB) with and without the addition of betamethasone for the acute pain management of patient's undergoing laparoscopic cholecystectomy (LC). Methods Eligible patients were allocated randomly to three groups: (A) general anesthesia followed by surgeon infiltration at port sites with ropivacaine (n = 48), (B) general anesthesia after single injection TPVB at right T7-8 level with ropivacaine only, Ropi_TPVB (n = 43), and (C) general anesthesia after single injection TPVB with ropivacaine plus betamethasone, Ropi_Betamet_TPVB (n = 45). Primary outcome was TPVB duration assessed by the number of dermatomes at regular intervals up to 72 hours (h). Secondary outcomes included pain scores, analgesics consumption, and perioperative functional outcomes. Results The addition of betamethasone to ropivacaine in TPVB resulted in similar onset but significantly slower block regression between 4 h and 72 h as compared to ropivacaine alone (P < 0.001). When compared to the surgeon infiltration group, Ropi_TPVB and Ropi_Betamet_TPVB group had significantly lower pain scores for 24 h and 48 h, respectively, P ≤ 0.001. Both TPVB groups had less frequency of analgesics administration for 72 h, P < 0.001, and earlier mobilization, P < 0.001. Conclusions The addition of betamethasone to TPVB significantly prolonged block duration as compared to local anesthetic alone. TPVB both with and without the addition of betamethasone resulted in better perioperative analgesia and improved functional status when compared to surgical site local anesthetic infiltration.
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http://dx.doi.org/10.7759/cureus.6023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886661PMC
October 2019

Non-Chloride in Situ Preparation of Nano-Cuprous Oxide and Its Effect on Heat Resistance and Combustion Properties of Calcium Alginate.

Polymers (Basel) 2019 Oct 27;11(11). Epub 2019 Oct 27.

College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.

With Cu complexes as precursors, nano-cuprous oxide was prepared on a sodium alginate template excluded of Cl and based on which the calcium alginate/nano-cuprous oxide hybrid materials were prepared by a Ca crosslinking and freeze-drying process. The thermal degradation and combustion behavior of the materials were studied by related characterization techniques using pure calcium alginate as a comparison. The results show that the weight loss rate, heat release rate, peak heat release rate, total heat release rate and specific extinction area of the hybrid materials were remarkably lower than pure calcium alginate, and the flame-retardant performance was significantly improved. The experimental data indicates that nano-cuprous oxide formed a dense protective layer of copper oxide, calcium carbonate and carbon by lowering the initial degradation temperature of the polysaccharide chain during thermal degradation and catalytically dehydrating to char in the combustion process, and thereby can isolate combustible gases, increase carbon residual rates, and notably reduce heat release and smoke evacuation.
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http://dx.doi.org/10.3390/polym11111760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918189PMC
October 2019

Biosynthesis and regulation mechanisms of the Pasteurella multocida capsule.

Res Vet Sci 2019 Dec 25;127:82-90. Epub 2019 Oct 25.

Laboratory of Veterinary Biologics Engineering, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China; Key-Disciplines Lab of Safety of Environment and Animal Product, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China. Electronic address:

Pasteurella multocida possesses a polysaccharide capsule composed of a viscous surface layer that acts as a critical structural component and virulence factor. Capsular polysaccharides are structurally similar to vertebrate glycosaminoglycans, providing an immunological mechanism for bacterial molecular mimicry, resistance to phagocytosis, and immune evasion during the infection process. In recent years, a series of important research advances have been made in understanding the biosynthesis and regulatory aspects of the P. multocida capsule. This review systematically examines the serogroups, polysaccharide composition and structures, biosynthetic loci and functions, biosynthesis pathways, and expression regulation mechanisms of the P. multocida capsule, supplying a theoretical basis for the molecular pathogenesis of the P. multocida capsule and the future development of capsular polysaccharide vaccines.
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http://dx.doi.org/10.1016/j.rvsc.2019.10.011DOI Listing
December 2019