Publications by authors named "Yun Wu"

659 Publications

Disrupted fronto-temporal function in panic disorder: a resting-state connectome study.

Brain Imaging Behav 2021 Oct 19. Epub 2021 Oct 19.

Nanjing Brain Hospital Affiliated to Nanjing Medical University, No.264 Guangzhou Road, Gulou District, Nanjing, 210029, China.

Recent neuroimaging studies have identified alterations in activity and connectivity among many brain regions as potential biomarkers for panic disorder. However, the functional connectome of panic disorder is not well understood. Therefore, a graph-theoretical approach was applied in this study to construct functional networks of patients and healthy controls in order to discover topological changes in panic disorder. 31 patients and 33 age and sex matched healthy controls underwent resting-state functional magnetic resonance imaging. Brain networks for each participant were structured using nodes from the Anatomical Automatic Labeling template and edges from connectivity matrices. Then, topological organizations of networks were calculated. Network-based statistical analysis was conducted, and global and nodal properties were compared between patients and controls. Unlike controls, patients with panic disorder displayed a small-world network. Patients also revealed decreased nodal efficiency in right superior frontal gyrus (SFG), middle frontal gyrus (MFG), right superior temporal gyrus (STG), and left middle temporal gyrus (MTG). Decreased functional connectivity was found in panic disorder between right MTG and extensive temporal regions. Among these disrupted regions, the decreased nodal efficiency of SFG showed a positive correlation with clinical symptoms while nodal betweenness centrality in angular gyrus showed a negative correlation. Our results indicated decreased function of global and regional information transmission in panic disorder and emphasized the role of temporal regions in its pathology.
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http://dx.doi.org/10.1007/s11682-021-00563-zDOI Listing
October 2021

Pteryxin attenuates LPS-induced inflammatory responses and inhibits NLRP3 inflammasome activation in RAW264.7 cells.

J Ethnopharmacol 2021 Oct 15:114753. Epub 2021 Oct 15.

Institute of Pharmaceutical Chemistry and Pharmacology, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia Autonomous Region, PR China; Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Tongliao, 028000, Inner Mongolia Autonomous Region, PR China. Electronic address:

Ethnopharmacological Relevance: Pteryxin is a natural coumarin compound that is found in "Qianhu", a traditional Chinese medicine, which possesses heat-clearing and detoxifying functions according to the theory of Traditional Chinese Medicine. Despite its medicinal effects, its anti-inflammatory and mechanisms of actions have not been established.

Aim Of This Study: This study aims to evaluate the anti-inflammatory property and reveal the possible anti-inflammatory mechanisms of pteryxin.

Material And Methods: LPS-induced RAW 264.7 macrophages and LPS-induced zebrafish model were used for the anti-inflammatory activity determination of pteryxin. The level of NO, PEG, TNF-α and IL-6 were measured by ELISA. The accumulation of NO and ROS was stained and observed by a fluorescence microscopy. The nuclear translocation of NF-κB p65 and formation of NLRP3 inflammasome complex in LPS-induced RAW 264.7 macrophage cells were analyzed by immunofluorescence assay. The expression level of iNOS, IL-6, COX-2, TNF-α, p-p38, p38, ERK, JNK, p-ERK, p-JNK, IKK, IκB-α, p-IKK, p-IκB-α, p65, NLRP3, p-p65, Caspase 1 (p 20), ASC, and GAPDH were determined by Western blotting.

Results: Lipopolysaccharide (LPS)-induced prostaglandin E2 (PGE2) and nitric oxide (NO) secretions were found to be downregulated by pteryxin. Moreover, pteryxin significantly suppressed inflammatory factor secretion in LPS-treated RAW 264.7 cells. Mechanistically, pteryxin significantly downregulated NF-κB/MAPK activation. Moreover, pteryxin inhibited caspase-1 and NLRP3 activation and formation of ASC specks in RAW 264.7 cells, implying that pteryxin inhibits inflammasome assembly, which is a signal for NLRP3 inflammasome activation. In conclusion, pteryxin blocks NF-κB/MAPK signaling, and suppresses the initiation and activation of NLRP3 thereby preventing inflammation.

Conclusion: Pteryxin is a potential treatment option for inflammatory-related diseases.
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http://dx.doi.org/10.1016/j.jep.2021.114753DOI Listing
October 2021

Economic Burden of Depressive Symptoms Conditions among Middle-Aged and Elderly People with Hypertension in China.

Int J Environ Res Public Health 2021 Sep 23;18(19). Epub 2021 Sep 23.

Department of Health Policy and Management, School of Public Health, Sun Yat-sen University, 74, Zhongshan 2nd Road, Guangzhou 510030, China.

People with hypertension are more prone to incur depressive symptoms, while depressive symptoms have an obvious influence on the healthy functioning, treatment, and management of hypertensive patients. However, there have been limited studies on the association between depression and the economic burden of hypertension. We used data from the 2018 China Health and Retirement Longitudinal Study (CHARLS) to estimate the additional annual direct and indirect economic burden of depressive symptoms among middle-aged and elderly hypertensive patients with a multivariable regression model. The depressive symptoms were associated with substantial additional direct and indirect economic burden. Compared with non-co-MHDS (non-co-morbid hypertension and depressive symptoms) patients, the direct economic burden of lower co-MHDS (co-morbid hypertension and depressive symptoms) patients and higher co-MHDS patients increased 1887.4 CNY and 5508.4 CNY, respectively. For indirect economic burden, the lower co-MHDS patients increased 331.2 CNY and the higher co-MHDS patients increased 636.8 CNY. Both direct and indirect economic burden were incremental with the aggravation of depressive symptoms. The results showed depressive symptoms increased total healthcare costs by increasing the utilization and expenditure of primary healthcare services. Depressive symptoms also led to economic loss of productivity, especially for agricultural workers. This study highlights the importance of mental healthcare for hypertensive patients.
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http://dx.doi.org/10.3390/ijerph181910009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508275PMC
September 2021

NaFePO(MoO): A Promising NASICON-Type Electrode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2021 Oct 11;13(41):48865-48871. Epub 2021 Oct 11.

School of Physics, Northwest University, Xi'an 710069, China.

Searching for polyanionic electrode materials with high Na and electronic conductivity is pivotal to realize high-performance sodium-ion batteries. Here, we report a novel polyanionic-based NASICON-type compound, NaFePO(MoO) (NFPM), that does not crystallize in the common space group -3 or 2/ but in the rare 2/. The studies on bond valence sum maps show that NFPM has high Na conductivity because the large volumes of MoO groups make the interstitial channels wider, thus making the energy barrier of Na diffusion decrease along these channels. Density functional theory calculations demonstrate that NFPM has high electronic conductivity because the contribution of Mo 4d orbitals on the formation of the bottom of the conduction band makes the connected MoO groups take part in electron transport. Electrochemical tests exhibit that NFPM can deliver a capacity of ∼80 mAh g with good reversible cyclability utilizing the Fe/Fe redox couple. In situ X-ray diffraction measurements indicate that NFPM undergoes one-phase reaction mechanism in the process of charge and discharge.
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http://dx.doi.org/10.1021/acsami.1c15010DOI Listing
October 2021

Small molecule compounds with good anti-inflammatory activity reported in the literature from 01/2009 to 05/2021: a review.

J Enzyme Inhib Med Chem 2021 Dec;36(1):2139-2159

Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Inner Mongolia, China.

Inflammation and disease are closely related. Inflammation can induce various diseases, and diseases can promote inflammatory response, and two possibly induces each other in a bidirectional loop. Inflammation is usually treated using synthetic anti-inflammatory drugs which are associated with several adverse effects hence are not safe for long-term use. Therefore, there is need for anti-inflammatory drugs which are not only effective but also safe. Several researchers have devoted to the research and development of effective anti-inflammatory drugs with little or no side effects. In this review, we studied some small molecules with reported anti-inflammatory activities and hence potential sources of anti-inflammatory agents. The information was retrieved from relevant studies published between January 2019 and May, 2021 for review. This review study was aimed to provide relevant information towards the design and development of effective and safe anti-inflammation agents.
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http://dx.doi.org/10.1080/14756366.2021.1984903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516162PMC
December 2021

Nondestructive detection model of soluble solids content of an apple using visible/near-infrared spectroscopy combined with CARS and MPGA.

Appl Opt 2021 Sep;60(27):8400-8407

Selecting the decisive characteristic variables is particularly important to analyze the soluble solids content (SSC) of an apple with visible/near-infrared spectroscopy (VIS-NIRS) technology. The multi-population genetic algorithm (MPGA) was applied to variable selection for the first time, to the best of our knowledge. A hybrid variable selection method combined competitive adaptive reweighted sampling (CARS) with MPGA (CARS-MPGA) was proposed. In this method, CARS was firstly used to shrink the variable space, and then the MPGA was used to further fine select the characteristic variables. Based on CARS-MPGA, a nondestructive quantitative detection SSC model of an apple was established and compared with the models established by different variable selection methods, such as successive projections algorithm, synergy interval partial least squares, and genetic algorithm. The experiments showed that the CARS-MPGA model was the best. The number of modeling variables was only 64, and the determination coefficients, root mean squared error, and residual predictive deviation for the prediction set were 0.853, 0.443, and 2.612, respectively. The results demonstrated that the CARS-MPGA is a reliable variable selection method and can be used for fast nondestructive detection SSC of an apple.
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http://dx.doi.org/10.1364/AO.439291DOI Listing
September 2021

Influential Factors and Efficacy Analysis of Tacrolimus Concentration After Allogeneic Hematopoietic Stem Cell Transplantation in Children with β-Thalassemia Major.

Pharmgenomics Pers Med 2021 24;14:1221-1237. Epub 2021 Sep 24.

Department of Pharmacy, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, People's Republic of China.

Purpose: To analyze factors influencing tacrolimus (TAC) trough concentration (C) in β-thalassemia major (β-TM) pediatric patients after allogeneic hematopoietic stem cell transplantation (Allo-HSCT) and to investigate the effects of genotype polymorphism and drug-drug interactions on TAC trough concentration in children with β-TM. Furthermore, to analyze the correlation between TAC C and efficacy and adverse reactions.

Patients And Methods: Prospectively collection of demographic information and details of combined treatment of patients with β-TM receiving HSCT, and genotypes of CYP3A4, CYP3A5, and ABCB1 (rs1045642, rs1128503, rs2032582) were obtained for each patient. Univariate analysis and multiple linear regression analysis were used to investigate influencing factors on TAC C. The impact of different genotypes and the co-administration of azole antifungal drugs on β-TM patients receiving TAC were evaluated, together with the correlation between acute graft-versus-host disease (aGVHD), infection, and liver injury of TAC C.

Results: A total of 46 patients with 587 concentration data were included. The multiple linear regression results showed that the patient's sex, weight, postoperative time, hemoglobin, platelet count, serum cystatin C, and combined voriconazole were independent influencing factors of the infusion trough concentration/daily dose, C/D. Age, body surface area, postoperative time, co-administration of voriconazole, and CYP3A4*18B are independent influencing factors of C/D. Group comparisons showed that voriconazole can affect TAC C administered intravenously (IV) and orally in β-TM pediatric patients, while patient genotype can affect TAC C during oral administration. TAC C does not correlate with aGVHD or liver injury, but infection may be associated with TAC C.

Conclusion: The concentration of TAC should be closely monitored when co-administered with voriconazole. It is worth considering that the influence of genotype on the trough concentration of oral TAC and individualized drug administration warrant investigation. Finally, this study indicated that C is not suitable as an indicator of the efficacy of TAC.
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http://dx.doi.org/10.2147/PGPM.S325103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478485PMC
September 2021

Rare novel LPL mutations are associated with neonatal onset lipoprotein lipase (LPL) deficiency in two cases.

BMC Pediatr 2021 09 20;21(1):414. Epub 2021 Sep 20.

Department of Neonatology, Hunan Children's Hospital, No.86 Ziyuan Road, Changsha, 410007, China.

Background: Lipoprotein lipase (LPL) deficiency is a monogenic lipid metabolism disorder biochemically characterized by hypertriglyceridemia (HTG) inherited in an autosomal recessive manner. Neonatal onset LPL deficiency is rare. The purpose of this study was to clarify the clinical features of neonatal LPL deficiency and to analyze the genetic characteristics of LPL gene.

Methods: In order to reach a definite molecular diagnose, metabolic diseases-related genes were sequenced through gene capture and next generation sequencing. Meanwhile, the clinical characteristics and follow-up results of the two newborns were collected and analyzed.

Results: Three different mutations in the LPL gene were identified in the two newborns including a novel compound heterozygous mutation (c.347G > C and c.472 T > G) and a reported homozygous mutation (c.836 T > G) was identified. Interestingly, both the two neonatal onset LPL deficiency patients presented with suffered recurrent infection in the hyperlipidemia stage, which was not usually found in childhood or adulthood onset LPL deficiency patients.

Conclusion: The two novel mutaitons, c.347G > C and c.472 T > G, identified in this study were novel, which expanded the LPL gene mutation spectrum. In addition, suffered recurrent infection in the hyperlipidemia stage implied a certain correlation between immune deficiency and lipid metabolism abnormality. This observation further supplemented and expanded the clinical manifestations of LPL deficiency.
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http://dx.doi.org/10.1186/s12887-021-02875-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451144PMC
September 2021

Early detection relationship of cerebral palsy markers using brain structure and general movements in infants born <32 weeks gestational age.

Early Hum Dev 2021 Aug 25;163:105452. Epub 2021 Aug 25.

Department of Rehabilitation, Children's Hospital, Fudan University, Shanghai 201102, China.

Aim: To detect early brain structural and clinical functional markers of brain injury and development based on a magnetic resonance imaging (MRI) scoring system and a general movement assessment (GMA) for preterm infants later diagnosed with cerebral palsy (CP).

Study Design: Retrospective cohort study. General movements (GMs) were scored according to a semiquantitative scoring system: the GMs optimality score (GMOS) at preterm and term ages and the Motor Optimality Score (MOS) at the corrected age of 3 months after birth. Brain magnetic resonance imaging (MRI) at term-equivalent age was scored using an MRI scoring system. We analyzed the relationship between the early degree of cerebral white matter (WM) abnormality and the GMOS and the MOS for infants born <32 weeks gestational age later diagnosed with CP in a comparison group of neurotypical controls.

Subjects: Sixteen preterm infants were included in this study who underwent MRI and GMs assessment. 8 out of the 16 preterm infants were later diagnosed with CP, while the other 8 infants with normal motor development (N) were placed into the control group. Their median gestational age was 30w6d and 27w6d for each group respectively.

Results: The cerebral WM MRI scores were significantly higher in the CP group than in the control group (p < 0.01). The GMOS and MOS were significantly higher in the control group than in the CP group (p < 0.05). The MOS showed a strong correlation to the cerebral WM MRI score (r = -0.88) and the subscale of cerebral WM items (the cystic degeneration and the focal signal abnormalities) of the MRI score (r = -0.94) in the CP group. The MOS also showed a correlation with corrected biparietal diameter (cBPD) in the preterm infant group with CP (r = 0.75). Results of linear regression analyses between term MRI and GMs measures in preterm infants with CP are presented. Cerebral WM scores were associated with the MOS (β = -0.63; 95%CI = -0.97, -0.29; p < 0.01). Cerebral WM injury, including the subscale of cystic degeneration and focal signal abnormalities was closely associated with the MOS (β = -0.83; 95%CI = -1.13, -0.54; p < 0.001).

Conclusion: Cerebral WM scores show a strong association with a decreased motor performance on the MOS in preterm infants later diagnosed with CP. Severe white matter injury and significantly decreased MOS scores may provide useful early markers and strong evidence to early predict the risk of later development of cerebral palsy in preterm infants.
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http://dx.doi.org/10.1016/j.earlhumdev.2021.105452DOI Listing
August 2021

First Report of Leaf Spot Disease On Microstegium vimineum Caused by Bipolaris setariae in China.

Plant Dis 2021 Sep 17. Epub 2021 Sep 17.

Tongwei RoadNanjing, China, 210095;

Microstegium vimineum, a Poaceae annual C4 plant, occurred widely in crop fields, tea gardens, orchards, under forests and roadsides in most provinces and regions south of the Yellow River, China. It was introduced into the eastern USA causing ecological and environmental damage (Stricker, 2016). In October 2015, M. vimineum plants with leaf spots were observed on the roadside of Mingling Road (32.04521°E, 118.84323°N), Nanjing, China. In an early stage of disease development, light brown or brown, round or oval shaped lesions appeared on the upper surface of leaves. In a middle stage, the lesions gradually expanded and the edges of the diseased leaves were lightly curled. In a late stage, leaves were withered or curled and the entire plant died. Initial disease incidence was up to 85% among natural populations of the weed. Diseased leaves collected from field were surface disinfected (75% ethanol for 30s; 1% sodium hypochlorite solution for 30s; 75% ethanol for 30s; sterile deionized water for 1min) and placed on water agar (20g agar per liter) (Kleczewski et al., 2010). Plates were incubated in the dark at 28℃ for 3 days. Following incubation, leaves, spores and conidiophores were examined using light microscopy. Single spores were obtained by using the single-spore procedure, plating out a loopful of spores onto water agar, and then carving individual spores out with associated agar under a microscope. Single spores were isolated, plated onto MV-agar (30g M. vimineum leaves, 20g agar per liter), and placed under 365 nm wavelength black light. Fungal colonies were transferred onto PDA medium, after 4 days colonies measured between 83 to 86 mm in diameter, appeared flat and dark brown, with short, light gray aerial hyphae. Conidiophores were solitary or clustered, light brown to medium brown, with pale apical color and multiple septa. The upper part was usually geniculated, 5.5-9.5 μm wide. Conidia were light yellowish brown to medium yellowish brown, mostly fusiform, straight or curved, fusoid or navicular, often slightly curved, rarely straight, smooth, 5-9 (mostly 7) septa, 48-70×10-14.5 μm (average 57×12.5 μm); hilium slightly prominent, and truncated at the base. Through morphological observation, the fungus was preliminarily identified as Bipolaris sp.. Four to five seeds of M. vimineum were planted in pots (10 cm in diameter) filled with nutrient soil, placed in the greenhouse and watered regularly. Four pots were inoculated with a conidia suspension of 1×105 sp/mL, at 4-5 true stage. Inoculated seedlings were maintained under 80% humidity and 28℃ for 24h in the dark, and then transferred to a greenhouse. Three pots of uninoculated seedlings were used as controls. Two days after the inoculation, buff-colored, irregular-shaped spots appeared centered on leaf veins. Within a week, diseased leaves became crinkled and their edges were yellow to brown due to proliferation of the spots. By 15 days, large areas of brown spots appeared on the leaves, some leaves turned yellow-brown and severely curled, and 80% of the plants had died. The diseased symptoms were similar to that of the field sample. The fungus re-isolated resulted morphologically identical to the original isolate grown on PDA medium and used for inoculation, thus fulfilled Koch's postulates. The CTAB method was used to extract DNA from isolates of diseased leaves taken directly from the field, and the internal transcribed spacer (ITS) and glyceraldehyde 3-phosphate dehydrogenase gene (GPDH) were amplified using primer pairs of ITS1/ITS4 and GPD/GPD2 (Manamgoda et al., 2014) respectively. The ITS amplified sequence (Genbank accession MW446193) shared 100% identity with the reference sequence of Bipolaris setariae (MN215638.1) and the GPDH amplified sequence (MW464364) shared 99.83% identity with the reference sequence of B. setariae (MK144540.1). Field experiments were conducted in Laboratory Base of Nanjing Agricultural University, where M. vimineum plants were planted. Spore suspensions with concentrations of 105, 104, 103, 102, and 101 sp/mL were prepared, distilled water was used for control, and there were four replicates of each treatment. Twenty four plots were randomly arranged, the experimental unit consisted of 50 to 60 plants in an area of 0.5m×0.6m. The interval distance between plots was about 20 cm so as to prevent the mutual influence among treatments. M. vimineum plants were inoculated at 3-4 true leaf stage. Inoculation was done at sunset, and 60 mL spore suspension was sprayed onto each plot. After spraying, the waterproof-breathable black cloth was used to cover the plots, and removed 36 hours later. The outdoor temperature was 20~28℃. After 10 days, the symptoms of M. vimineum were observed and the disease index was recorded. SPSS 20 software (SPSS Inc., Chicago, IL, USA) was used for variance analysis, and Origin 9.0 (OriginLab, Hampton, MA, USA) was used to calculate the half lethal concentration (ED50) and 90% lethal concentration (ED90) of the strain MLL-1-5 on M. vimineum. Symptoms appeared on inoculated M. vimineum seedlings immediately after dark treatment. Within a week, all seedlings inoculated with the highest spore concentration were dead. Plants sprayed with water remained healthy. ED50 and ED90 of the strain MLL-1-5 was 1.9×101 and 1.4×103 sp/mL respectively, which indicated aggressiveness of the strain MLL-1-5 B. setariae. After 28 days, infected M. vimineum plants did not recover. This is the first report of leaf spot disease on M. vimineum caused by B. setariae in China. M. vimineum is a widely distributed and extremely harmful weed in China and United States. No biocontrol agents against M. vimineum are currently available. B. setariae may have potential as a biocontrol agent against M. vimineum both in China and the United States.
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http://dx.doi.org/10.1094/PDIS-04-21-0703-PDNDOI Listing
September 2021

Acetate and electricity generation from methane in conductive fiber membrane- microbial fuel cells.

Sci Total Environ 2021 Sep 4;804:150147. Epub 2021 Sep 4.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou, China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, China. Electronic address:

Microbial conversion of methane to electricity, fuels, and liquid chemicals has attracted much attention. However, due to the low solubility of methane, it is not considered a suitable substrate for microbial fuel cells (MFCs). In this study, a conductive fiber membrane (CFM) module was constructed as the bioanode of methane-driven MFCs, directly delivering methane. After biofilm formation on the CFM surface, a steady voltage output of 0.6 to 0.7 V was recorded, and the CFM-MFCs obtained a maximum power density of 64 ± 2 mW/m. Moreover, methane oxidation produced a high concentration of intermediate acetate (up to 7.1 mM). High-throughput 16S rRNA gene sequencing suggests that the microbial community was significantly changed after electricity generation. Methane-related archaea formed a symbiotic consortium with characterized electroactive bacteria and fermentative bacteria, suggesting a combination of three types of microorganisms for methane conversion into acetate and electricity.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150147DOI Listing
September 2021

Topical analgesic BENGAY® reduces myocardial infarct size in rodents.

Br J Anaesth 2021 Nov 6;127(5):e163-e166. Epub 2021 Sep 6.

Department of Anesthesiology, Perioperative, and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.bja.2021.07.029DOI Listing
November 2021

Driving mechanism of drift-step-recovery diodes.

Rev Sci Instrum 2021 Aug;92(8):084702

Science and Technology on Plasma Laboratory, Air Force Engineering University, Xi'an 710038, People's Republic of China.

Parameters governing forward and reverse pumping of a device mechanism have an important influence on the cutoff and output characteristics of a drift-step-recovery diode (DSRD). Therefore, we analyzed the driving circuit for the DSRD that was used to adjust pumping parameter values. With multiple modules in parallel, a driving method exploiting narrow-pulse pumping is proposed in developing a high-repetition-frequency DSRD pulse generator. In experiments and simulations, one key factor affecting the working stability of this pulse generator operating at repetition frequencies in the megahertz range was found that helped in further optimizing the parameter settings of our 2 kV DSRD driving circuit. From this insight, a 2 kV-5 MHz all-solid-state high-repetition-frequency pulse generator based on the DSRD was developed.
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http://dx.doi.org/10.1063/5.0048768DOI Listing
August 2021

NP-ALT, a liposomal:peptide drug, blocks p27Kip1 phosphorylation to induce oxidative stress, necroptosis and regression in therapy-resistant breast cancer cells.

Mol Cancer Res 2021 Aug 26. Epub 2021 Aug 26.

Cell Biology and Pediatrics, SUNY Downstate Medical Center

Resistance to cyclin D-cdk4/6 inhibitors (CDK4/6i) represents an unmet clinical need and is frequently caused by compensatory CDK2 activity. Here we describe a novel strategy to prevent CDK4i resistance by using a therapeutic liposomal:peptide formulation, NP-ALT, to inhibit the tyrosine phosphorylation of p27Kip1(CDKN1B), which in turn inhibits both CDK4/6 and CDK2. We find that NP-ALT blocks proliferation in HR+ breast cancer (BC) cells, as well as CDK4i-resistant cell types, including Triple Negative (TN) BC. The peptide ALT is not as stable in primary mammary epithelium, suggesting that NP-ALT has little effect in non-tumor tissues. In HR+ BC cells specifically, NP-ALT treatment induces ROS and RIPK1-dependent necroptosis. Estrogen signaling and ER alpha appear required. Significantly, NP-ALT induces necroptosis in MCF7 ESRY537S cells, which contain an ER gain of function mutation frequently detected in metastatic patients, which renders them resistant to endocrine therapy. Here we show that NP-ALT causes necroptosis and tumor regression in treatment naïve, palbociclib-resistant and endocrine-resistant BC cells and xenograft models, demonstrating that p27 is a viable therapeutic target to combat drug resistance. Implications: This study reveals that blocking p27 tyrosine phosphorylation inhibits CDK4 and CDK2 activity and induces ROS-dependent necroptosis, suggesting a novel therapeutic option for endocrine and CDK4 inhibitor-resistant HR+ tumors.
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http://dx.doi.org/10.1158/1541-7786.MCR-21-0081DOI Listing
August 2021

Maternal genetic history of southern East Asians over the past 12,000 years.

J Genet Genomics 2021 Jun 21. Epub 2021 Jun 21.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, Beijing 100044, China; University of the Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Southern East Asia, including Guangxi and Fujian provinces in China, is home to diverse ethnic groups, languages, and cultures. Previous studies suggest a high complexity regarding population dynamics and the history of southern East Asians. However, large-scale genetic studies on ancient populations in this region are hindered by limited sample preservation. Here, using highly efficient DNA capture techniques, we obtain 48 complete mitochondrial genomes of individuals from Guangxi and Fujian in China and reconstruct their maternal genetic history over the past 12,000 years. We find a strong connection between southern East Asians dating to ~12,000-6000 years ago and present-day Southeast Asians. In addition, stronger genetic affinities to northern East Asians are observed in historical southern East Asians than Neolithic southern East Asians, suggesting increased interactions between northern and southern East Asians over time. Overall, we reveal dynamic connections between ancient southern East Asians and populations located in surrounding regions, as well as a shift in maternal genetic structure within the populations over time.
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http://dx.doi.org/10.1016/j.jgg.2021.06.002DOI Listing
June 2021

NCAPG promotes the progression of lung adenocarcinoma via the TGF-β signaling pathway.

Cancer Cell Int 2021 Aug 21;21(1):443. Epub 2021 Aug 21.

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China.

Background: Lung cancer has the highest case fatality rate among cancers because of uncontrolled proliferation and early metastasis of cancer cells in the lung tissue. This study aimed to clarify the role of the non-SMC condensin I complex, subunit G (NCAPG) in lung adenocarcinoma (LUAD), explore the mechanisms of its progression, and lay the foundation for the search for new biological markers.

Methods: We analyzed overlapping differentially expressed genes (DEGs) from three datasets; a protein-protein interaction (PPI) network was subsequently constructed and analyzed using Cytoscape. We then selected NCAPG for validation because of its poor prognosis and because it has not been sufficiently studied in the context of LUAD. Immunohistochemical analysis was used to detect the expression of NCAPG in LUAD tissues, and the relationships between NCAPG and clinical parameters were analyzed. In vitro and in vivo experiments were conducted to verify the role of NCAPG in LUAD. Finally, we studied the specific mechanism of action of NCAPG in LUAD.

Results: Through comprehensive analysis of the GSE43458, GSE75037, and The Cancer Genome Atlas databases, we identified 517 overlapping DEGs. Among them, NCAPG was identified as a hub gene. Immunohistochemical analysis revealed that NCAPG was strongly associated with the clinical stage, M-classification, and N-classification. Univariate and multivariate Cox regression analyses indicated that NCAPG was a prognostic risk factor for LUAD, while the in vitro experiments showed that NCAPG overexpression promoted proliferation, migration, invasion, and epithelial-mesenchymal transition. Furthermore, knockdown of NCAPG inhibited LUAD progression, both in vitro and in vivo. Mechanistically, NCAPG overexpression increased p-Smad2 and p-Smad3 expressions in the transforming growth factor β (TGF-β) signaling pathway. Additionally, rescue experiments indicated that TGF-β signaling pathway inhibitors could restore the effect of NCAPG overexpression in LUAD cells.

Conclusions: NCAPG may promote proliferation and migration via the TGF-β signaling pathway in LUAD.
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http://dx.doi.org/10.1186/s12935-021-02138-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380402PMC
August 2021

Design and Implementation of a Modular and Scalable Research Platform for Ultrasound Computed Tomography.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Aug 19;PP. Epub 2021 Aug 19.

Increasing attention has been attracted to the research of ultrasound computed tomography (USCT). This paper reports the design considerations and implementation details of a novel USCT research system named UltraLucid, which aims to provide a user-friendly platform for researchers to develop new algorithms and conduct clinical trials. The modular design strategy is adopted to make the system highly scalable. A prototype has been assembled in our laboratory, which is equipped with a 2048-element ring transducer, 1024 transmit (TX) channels, 1024 receive (RX) channels, two servers, and a control unit. The prototype can acquire raw data from 1024 channels simultaneously using a modular data acquisition and transfer system, consisting of sixteen excitation and data acquisition (EDAQ) boards. Each EDAQ board has 64 independent TX and RX channels and 4 Gigabit Ethernet interfaces for raw data transmission. The raw data can be transferred to two servers at a theoretical rate of 64 Gbps. Both servers are equipped with a 10.9 TB solid-state drive (SSD) array that can store raw data for offline processing. Alternatively, after processing by onboard field-programmable gate arrays (FPGAs), the raw data can be processed online using multi-core central processing units (CPUs) and graphics processing units (GPUs) in each server. Through a control software running on the host computer, the researchers can configure parameters for transmission, reception, and data acquisition. Novel transmit-receive scheme and coded imaging can be implemented. The modular hardware structure and the software-based processing strategy make the system highly scalable and flexible. The system performance is evaluated with phantoms and in vivo experiments.
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http://dx.doi.org/10.1109/TUFFC.2021.3105691DOI Listing
August 2021

Up-regulated microRNA-132 reduces the cognition-damaging effect of sevoflurane on Alzheimer's disease rats by inhibiting FOXA1.

Genomics 2021 Aug 13;113(6):3644-3652. Epub 2021 Aug 13.

Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, Heilongjiang, People's Republic of China. Electronic address:

Objective: Some studies have implied the damaging effect of sevoflurane (sevo) on cognitive function in Alzheimer's disease (AD). This research was conducted to explore the effect of microRNA (miR)-132/forkhead-box A1 (FOXA1) axis on cognitive ability of sevo-treated AD rats.

Methods: The condensed-matter Aβ-induced AD rats were injected with miR-132- or FOXA1-related plasmids, followed by inhalation with 3% sevo. Then, the cognitive functions of AD rats were assessed. miR-132 and FOXA1 levels in hippocampal tissues of AD rats, and their interaction were identified.

Results: miR-132 expression was reduced and FOXA1 mRNA and protein levels were elevated in AD rats. miR-132 targeted FOXA1. Sevo treatment impaired cognitive function in AD rats. Elevated miR-132 or inhibited FOXA1 attenuated sevo-mediated injury in AD rats. Overexpressed FOXA1 rescued the effect of elevated miR-132 in AD rats with sevo treatment.

Conclusion: Up-regulated miR-132 reduces the cognition-damaging effect of sevo on AD rats by inhibiting FOXA1.
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http://dx.doi.org/10.1016/j.ygeno.2021.08.011DOI Listing
August 2021

FBXW7 gene polymorphism is associated with type 2 diabetes in the Uygur population in Xinjiang, China.

Hereditas 2021 Aug 9;158(1):27. Epub 2021 Aug 9.

Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, P.R. China.

Background: FBXW7 gene expression is positively correlated with glycolipid metabolism and is associated with diabetes in animal models. In the current study, we focused on exploring whether genetic variants of the FBXW7 gene were associated with type 2 diabetes (T2DM) and the risk factors for T2DM in Uygur people in Xinjiang, China.

Methods: A total of 2164 Chinese Uygur subjects (673 T2DM patients and 1491 controls) were recruited for our case-control study, and four SNPs (rs10033601, rs2255137, rs2292743 and rs35311955) of the FBXW7 gene were genotyped using the improved multiplex ligation detection reaction (iMLDR) technique.

Results: Our study showed that the genotypes using the overdominant model (GA vs AA + GG) of rs10033601 and using the overdominant model (TA vs TT + AA) of rs2292743 were significantly different between T2DM patients and the controls (P = 0.005 and P = 0.012, respectively). After multivariate adjustments for confounders, the rs10033601 and rs2292743 SNPs were still independent risk factors for T2DM [GA vs AA + GG: odds ratio = 1.35, 95% confidence interval (CI) = 1.12-1.64, P = 0.002; TA vs TT + AA: OR = 1.28, 95% CI = 1.06-1.55, P = 0.011]. Participants within the Chinese Uygur populations and who with the GA genotype of rs10033601 and the TA genotype of rs2292743 were associated with significantly elevated glucose levels.

Conclusions: Our study revealed that both rs10033601 and rs2292743 of the FBXW7 gene were associated with T2DM in the Uygur populations in Xinjiang.
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http://dx.doi.org/10.1186/s41065-021-00191-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351158PMC
August 2021

Effects of COVID-19 on Mental Health and Anxiety of Adolescents Aged 13-16 Years: A Comparative Analysis of Longitudinal Data From China.

Front Psychiatry 2021 20;12:695556. Epub 2021 Jul 20.

Department of Clinical Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, China.

Adolescence is an important stage of psychological development, and the psychological and mental problems of many adults are affected by the COVID-19 epidemic. The aim of this study was to understand the psychological status of this group during the epidemic, and to determine the risk factors leading to psychological stress, as well as protective factors. An online survey was run on April 2, 2020. The participants were 254 adolescents aged 13-16 years from a junior high school in Jiangsu, China. The results were compared with the pre-epidemic data, which came from the psychological status survey routinely carried out by the school. Mental health variables were assessed via the Mental Health Test that included one validity subscale and eight content subscales. The number of adolescents with poor mental health increased significantly from 12.3 to 24.2%. There was significant increase in learning anxiety (33.7 vs. 56.4%), sensitivity tendency (19.8 vs. 46%), somatic anxiety (13.9 vs. 40.7%) and phobia tendency (4.4 vs. 10.1%). During the epidemic, there were significant differences between adolescents with normal and poor mental health in family structure, personality, relationship with siblings, daily exercise time, and risk of family members coming in contact with COVID-19. Living in stem family, no siblings, and risk of contracting COVID-19 from family members were significant risk factors for teenagers with poor mental health. Risk of contracting COVID-19 from family members was the most influential risk factor for learning anxiety, self-blaming tendency, sensitivity tendency, and somatic anxiety. Exercising for ≥1 h per day was a significant protective factor for poor mental health. During the COVID-19 epidemic, adolescents aged 13-16 years have had psychosocial problems, especially learning anxiety, sensitivity tendency, somatic anxiety, and phobia tendency, as well as risk factors for developing them. Our study provides insights for potential interventions.
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http://dx.doi.org/10.3389/fpsyt.2021.695556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330831PMC
July 2021

Determination of six parabens in biological samples by magnetic solid-phase extraction with magnetic mesoporous carbon adsorbent and UHPLC-MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Aug 3;1179:122817. Epub 2021 Jul 3.

School of Biology and Chemistry, Key Laboratory of Chemical Synthesis and Environmental Pollution Control-Remediation Technology of Guizhou Province, Xingyi Normal University for Nationalities, Xingyi 562400, PR China. Electronic address:

Although parabens are useful due to their antiseptic properties, their widespread use has caused concerns regarding their potential toxicological effects. In this study, a novel magnetic solid-phase extraction combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (MSPE-UHPLC-MS/MS) was developed, based on ordered magnetic mesoporous carbon (MMC), for paraben analysis. The MMC was prepared by soft-template synthesis, with a unique pore structure and a highly specific surface response, indicating potential as an excellent adsorbent. Several parameters affecting the paraben extraction efficiency were investigated and a novel method for paraben analysis in serum and urine samples using MSPE-UHPLCMS/MS was developed. The concentrations of methylparaben, ethylparaben, isopropylparaben, and propylparaben in these samples were 0.0380-4.36, 0.460-9.65, 0.0118-0.770, and 0.0363-0.641 μg/L, respectively, whereas isobutylparaben and butylparaben were not detected. Furthermore, satisfactory recoveries of 76.4-121% with relative standard deviations (n = 5) of 1.9-8.6% were obtained. Therefore, the developed MSPE-UHPLC-MS/MS method was efficient, highly sensitive, and reliable for analysing parabens in complex biological samples.
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http://dx.doi.org/10.1016/j.jchromb.2021.122817DOI Listing
August 2021

The mechanism of myocardial fibrosis is ameliorated by myocardial infarction-associated transcript through the PI3K/Akt signaling pathway to relieve heart failure.

J Int Med Res 2021 Jul;49(7):3000605211031433

Cardiology Department, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia Autonomous Region, China.

Objective: This study aimed to investigate the role of long noncoding RNA (LncRNA) myocardial infarction-associated transcript (MIAT) in a heart failure (HF) model and by regulating the PI3K/Akt signaling pathway.

Methods: We established HF models and and evaluated the collagen content of these models and other factors.

Results: We found that when LncRNA MIAT was silenced, vascular endothelial growth factor, phosphorylated protein kinase B (Akt), and phosphorylated phosphoinositide 3-kinase (PI3K) mRNA and protein levels were significantly downregulated, which suggested that MIAT activated the PI3K/Akt signaling pathway. Akt and PI3K expression was not significantly changed. We also found that when LncRNA MIAT was silenced, collagen expression was significantly downregulated. This finding suggested that MIAT promoted myocardial fibrosis during the development of HF. The levels of inflammatory factors were also significantly reduced with silencing of LncRNA MIAT. This finding suggested that MIAT promoted the expression of inflammatory factors in myocardial fibrosis by activating the PI3K/Akt signaling pathway.

Conclusion: This study indicates that silencing LncRNA MIAT may improve myocardial fibrosis and alleviate HF through the PI3K/Akt signaling pathway, which may be helpful for patients with HF to obtain a better therapeutic effect.
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http://dx.doi.org/10.1177/03000605211031433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293849PMC
July 2021

Identification of Potential Key Genes and Regulatory Markers in Essential Thrombocythemia Through Integrated Bioinformatics Analysis and Clinical Validation.

Pharmgenomics Pers Med 2021 5;14:767-784. Epub 2021 Jul 5.

Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, People's Republic of China.

Introduction: Essential thrombocytosis (ET) is a group of myeloproliferative neoplasms characterized by abnormal proliferation of platelet and megakaryocytes. Research on potential key genes and novel regulatory markers in essential thrombocythemia (ET) is still limited.

Methods: Downloading array profiles from the Gene Expression Omnibus database, we identified the differentially expressed genes (DEGs) through comprehensive bioinformatic analysis. GO, and REACTOME pathway enrichment analysis was used to predict the potential functions of DEGs. Besides, constructing a protein-protein interaction (PPI) network through the STRING database, we validated the expression level of hub genes in an independent cohort of ET, and the transcription factors (TFs) were detected in the regulatory networks of TFs and DEGs. And the candidate drugs that are targeting hub genes were identified using the DGIdb database.

Results: We identified 63 overlap DEGs that included 21 common up-regulated and 42 common down-regulated genes from two datasets. Functional enrichment analysis shows that the DEGs are mainly enriched in the immune system and inflammatory processes. Through PPI network analysis, , and were selected as hub genes. Interestingly, we found that the dysregulated hub genes are also aberrantly expressed in a bone marrow cohort of ET. Moreover, we found that the expression of , and genes were significantly under-expressed in ET (<0.05), which is consistent with our bioinformatics analysis. The ROC curve analysis also shows that these hub genes have good diagnostic value. Besides, we identified 4 TFs (SPI1, IRF4, SRF, and AR) as master transcriptional regulators that were associated with regulating the DEGs in ET. Cyclophosphamide, prednisone, fluorouracil, ruxolitinib, and lenalidomide were predicted as potential candidate drugs for the treatment of ET.

Discussion: These dysregulated genes and predicted key regulators had a significant relationship with the occurrence of ET with affecting the immune system and inflammation of the processes. Some of the immunomodulatory drugs have potential value by targeting , and genes in the treatment of ET.
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http://dx.doi.org/10.2147/PGPM.S309166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275175PMC
July 2021

Inflammatory monocytes promote pre-engraftment syndrome and tocilizumab can therapeutically limit pathology in patients.

Nat Commun 2021 07 6;12(1):4137. Epub 2021 Jul 6.

Department of Hematology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Unrelated cord blood transplantation (UCBT) is an effective treatment for hematopoietic disorders. However, this attractive approach is frequently accompanied by pre-engraftment syndrome (PES), severe cases of PES are associated with enhanced mortality and morbidity, but the pathogenesis of PES remains unclear. Here we show that GM-CSF produced by cord blood-derived inflammatory monocytes drives PES pathology, and that monocytes are the main source of IL-6 during PES. Further, we report the outcome of a single arm, single-center clinical study of tocilizumab in the treatment of steroid-refractory severe PES patients (www.chictr.org.cn ChiCTR1800015472). The study met the primary outcome measure since none of the patients was nonrelapse death during the 100 days follow-up. The study also met key secondary outcomes measures of neutrophil engraftment and hematopoiesis. These findings offer a therapeutic strategy with which to tackle PES and improve nonrelapse mortality.
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http://dx.doi.org/10.1038/s41467-021-24412-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260612PMC
July 2021

Self-compassion and the Mitigation of Negative Affect in the Era of Social Distancing.

Mindfulness (N Y) 2021 Jun 28:1-12. Epub 2021 Jun 28.

Department of Psychology, Tsinghua University, Beijing, China.

Objectives: The outbreak of contagious diseases and their associated non-pharmaceutical interventions can lead to negative mental health consequences. This study aimed to investigate online self-compassion exercises' effectiveness in alleviating people's negative affect (anxiety and negative emotions) during the COVID-19 pandemic lockdown.

Methods: Study 1 examined the associations between self-compassion and negative affect using an anonymous online survey. In study 2, two pilot experiments were conducted to examine online self-compassion exercises' (i.e., instructor-guided meditation and self-guided writing) effectiveness to mitigate individuals' negative affect. In study 3, a randomized controlled study was conducted to further examine online self-compassion writing's effectiveness in reducing people's negative affect.

Results: The results of study 1 indicated a significant negative association between self-compassion and participants' negative affect. Participants in study 2a reported significant decreases in negative affect after completing the self-compassion meditation. Study 2b showed that participants who completed the self-compassion writing reported significantly more self-compassion and less anxiety when compared to participants who did not. Study 3 showed substantial pre/post-test changes in participants who completed self-compassion writing. Importantly, there were significant increases in participants' self-compassion and decreases in negative affect when compared to participants in the control condition.

Conclusions: Self-compassion exercises were effective to alleviate individuals' anxiety and reduce negative emotions (even within a brief session) during the pandemic lockdown.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12671-021-01674-w.
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http://dx.doi.org/10.1007/s12671-021-01674-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236748PMC
June 2021

A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis.

Mol Plant 2021 Sep 30;14(9):1554-1568. Epub 2021 Jun 30.

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China; Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 5100642, China. Electronic address:

Phosphorus (P) is an indispensable macronutrient required for plant growth and development. Natural phosphate (Pi) reserves are finite, and a better understanding of Pi utilization by crops is therefore vital for worldwide food security. Ammonium has long been known to enhance Pi acquisition efficiency in agriculture; however, the molecular mechanisms coordinating Pi nutrition and ammonium remains unclear. Here, we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein, STOP1, in the nuclei of Arabidopsis root cells under Pi deficiency. We show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root surface. Rhizosphere acidification-triggered STOP1 accumulation activates the excretion of organic acids, which help to solubilize Pi from insoluble iron or calcium phosphates. Ammonium uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic levels. Taken together, we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate sources. These findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil.
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http://dx.doi.org/10.1016/j.molp.2021.06.024DOI Listing
September 2021

Exploring Misclassification Information for Fine-Grained Image Classification.

Sensors (Basel) 2021 Jun 18;21(12). Epub 2021 Jun 18.

Fujian Key Laboratory of Pattern Recognition and Image Understanding, Xiamen 361024, China.

Fine-grained image classification is a hot topic that has been widely studied recently. Many fine-grained image classification methods ignore misclassification information, which is important to improve classification accuracy. To make use of misclassification information, in this paper, we propose a novel fine-grained image classification method by exploring the misclassification information (FGMI) of prelearned models. For each class, we harvest the confusion information from several prelearned fine-grained image classification models. For one particular class, we select a number of classes which are likely to be misclassified with this class. The images of selected classes are then used to train classifiers. In this way, we can reduce the influence of irrelevant images to some extent. We use the misclassification information for all the classes by training a number of confusion classifiers. The outputs of these trained classifiers are combined to represent images and produce classifications. To evaluate the effectiveness of the proposed FGMI method, we conduct fine-grained classification experiments on several public image datasets. Experimental results prove the usefulness of the proposed method.
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http://dx.doi.org/10.3390/s21124176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235489PMC
June 2021

Human population history at the crossroads of East and Southeast Asia since 11,000 years ago.

Cell 2021 Jul 24;184(14):3829-3841.e21. Epub 2021 Jun 24.

Yunnan Institute of Cultural Relics and Archaeology, Kunming 650118, China; Yunnan Key Laboratory of Earth System Science, Yunnan University, Kunming 650500, China. Electronic address:

Past human genetic diversity and migration between southern China and Southeast Asia have not been well characterized, in part due to poor preservation of ancient DNA in hot and humid regions. We sequenced 31 ancient genomes from southern China (Guangxi and Fujian), including two ∼12,000- to 10,000-year-old individuals representing the oldest humans sequenced from southern China. We discovered a deeply diverged East Asian ancestry in the Guangxi region that persisted until at least 6,000 years ago. We found that ∼9,000- to 6,000-year-old Guangxi populations were a mixture of local ancestry, southern ancestry previously sampled in Fujian, and deep Asian ancestry related to Southeast Asian Hòabìnhian hunter-gatherers, showing broad admixture in the region predating the appearance of farming. Historical Guangxi populations dating to ∼1,500 to 500 years ago are closely related to Tai-Kadai and Hmong-Mien speakers. Our results show heavy interactions among three distinct ancestries at the crossroads of East and Southeast Asia.
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http://dx.doi.org/10.1016/j.cell.2021.05.018DOI Listing
July 2021

Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats.

BMC Complement Med Ther 2021 Jun 22;21(1):173. Epub 2021 Jun 22.

Department of Anatomy, Hubei University of Medicine, Shiyan, 442000, China.

Background: An emerging body of evidence indicates that puerarin (PUE) plays an important role in the treatment of angina pectoris, myocardial ischemia-reperfusion injury, hypertension and other cardiovascular diseases, but how PUE affects the vascular remodeling of hypertensive rats has not been reported yet. This study aimed to investigate the effect and mechanism of PUE on carotid arteries of spontaneously hypertensive rats (SHR) to provide the basis for the clinical application of PUE.

Methods: Thirty male SHR and six male Wistar Kyoto rats (WKY) aged 3 months were used in this study, SHR rats were randomly divided into 5 groups, PUE(40 or 80 mg/kg/d, ip) and telmisartan (TELMI) (30 mg/kg/d, ig) were administrated for 3 months. We use DMT myography pressure-diameter system to investigate biomechanical properties of carotid arteries, 10 μM pan-classical transient receptor potential channels (TRPCs) inhibitor SKF96365, 200 nM specific TRPC6 inhibitor SAR7334 and 100 μM Orai1 inhibitor ANCOA4 were used in the mechanical test.

Results: PUE can significantly decrease systolic and diastolic blood pressure, long-term administration of PUE resulted in a mild reduction of thickness and inner diameter of carotid artery. PUE ameliorate NE-response and vascular remodeling mainly through inhibiting TRPCs channel activities of VSMC.

Conclusion: PUE can ameliorate biomechanical remodeling of carotid arteries through inhibiting TRPCs channel activities of VSMC in spontaneously hypertensive rats.
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http://dx.doi.org/10.1186/s12906-021-03345-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216761PMC
June 2021

Identification and validation of inferior prognostic genes associated with immune signatures and chemotherapy outcome in acute myeloid leukemia.

Aging (Albany NY) 2021 06 18;13(12):16445-16470. Epub 2021 Jun 18.

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China.

Acute myeloid leukemia (AML) is a group of heterogeneous hematological malignancies. We identified key genes as and lncRNA through different bioinformatics tools. Furthermore, qPCR was performed to verify the expression level of essential genes in clinical samples. Retrospective research on 179 AML cases was used to investigate the relationship between the expression of and the characteristics of AML. The critical gene relationship with immune infiltration in AML was estimated. The clinical validation and prognostic investigation showed that , , and are highly expressed in AML ( < 0.001) and significantly associated with the overall survival in AML. Moreover, the retrospective research on 179 clinical cases showed that positive expression of is substantially related to AML classification ( < 0.001), higher count of white blood cells ( < 0.01), and poor chemotherapy outcome ( < 0.05). Furthermore, based on grouping as the high and low expression in TCGA-LAML profile, we found that genes in the highly expressed group are mainly involved in immune infiltration and inflammation-related signaling pathways. Finally, we discovered that the expression level of and lncRNA are not just closely related to the immune score and stromal score ( < 0.001) but also significantly positively correlated with various Immune signatures in AML ( < 0.001), indicating the association of these genes with immunosuppression in AML. The prediction of candidate drugs indicated that certain immunosuppressive drugs have potential therapeutic effects for AML. The critical genes could be used as potential biomarkers to evaluate the survival and prognosis of AML.
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http://dx.doi.org/10.18632/aging.203166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266366PMC
June 2021
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