Publications by authors named "Yun Ling"

422 Publications

Tissue distribution study of perfluorooctanoic acid in exposed zebrafish using MALDI mass spectrometry imaging.

Environ Pollut 2021 Nov 13;293:118505. Epub 2021 Nov 13.

Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.

Perfluorooctanoic acid (PFOA) as an emerging environmental contaminant, has become ubiquitous in the environment. It is of significance to study bioconcentration and tissue distribution of aquatic organisms for predicting the persistence of PFOA and its adverse effects on the environment and human body. However, the distribution of PFOA in different tissues is a complex physiological process affected by many factors. It is difficult to be accurately described by a simple kinetic model. In present study, a new strategy was introduced to research the PFOA distribution in tissues and estimate the exposure stages. Zebrafish were continuously exposed to 25 mg/L PFOA for 30 days to simulate environmental process. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) method was used to monitor the spatio-temporal distribution of PFOA in zebrafish tissues. By analyzing the law of change obtained from the high spatial resolution MSI data, two different enrichment trends in ten tissues were summarized by performing curve fitting. Analyzing the ratio of two types of curves, a new "exposure curve" was defined to evaluate the exposure stages. With this model, three levels (mild, moderate, and deep pollution stage) of PFOA pollution in zebrafish can be simply evaluated.
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http://dx.doi.org/10.1016/j.envpol.2021.118505DOI Listing
November 2021

Enhanced photocatalytic CO-reduction activity to form CO and CH on S-scheme heterostructured ZnFeO/BiMoO photocatalyst.

J Colloid Interface Sci 2021 Oct 15. Epub 2021 Oct 15.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou China; School of Chemistry and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou, Jiangxi 341000, China. Electronic address:

A novel ZnFeO/BiMoO heterojunction photocatalyst was synthesized by a facile solvothermal route. The incorporation of a narrow bandgap ZnFeO photocatalyst can efficiently improve the range of light response and light absorption capacity of the BiMoO via the formation of a hybrid structure at the interface. The formed hybrid interface facilitates the separation efficiency of photo-generated carriers at ZnFeO/BiMoO heterojunction significantly. The experimental results confirm that ZnFeO/BiMoO-20% heterojunction showed the highest photocatalytic efficiency for CO reduction. Specifically, the total product yield of 47.1 μmol g under 5 h simulated sunlight irradiation is measured in the counterparts of pure ZnFeO (14.79 μmol g) and pure BiMoO (19.01 μmol g). Indeed, the formation of ZnFeO/BiMoO heterojunction improved the photocatalytic efficiency for CO reduction.
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http://dx.doi.org/10.1016/j.jcis.2021.10.053DOI Listing
October 2021

N, P, O co-doped carbon filling into carbon nitride microtubes to promote photocatalytic hydrogen production.

Sci Total Environ 2021 Oct 21:151114. Epub 2021 Oct 21.

Shandong Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Liaocheng University, Liaocheng 252059, China. Electronic address:

Carbon nitride (CN) as the photocatalytic hydrogen production catalyst has attracted great attentions but suffering from a poor performance due to the unsatisfied energy band gap and the low separation efficiency of photogenerated carriers. Herein, we create a simple method to construct a novel CN-based photocatalyst, i.e., the N, P, O co-doped carbon filled CN microtube, which presents a narrow band gap, a high separation efficiency of photogenerated carriers, and a good stability. In this novel structure, the tubular morphology of CN ensures a narrow band gap, and the N, P, O co-doped carbon facilitates the transfer of photogenerated electrons. Coupling these two further reduces the energy band gap and improves the separation efficiency. For the photocatalytic hydrogen evolution under the visible light, the optimal sample presents an ultrahigh hydrogen evolution rate of 1149.71 μmol g h ranking at the top level, which is 112.60 times that of traditional bulk CN. In addition, it also has a high reusability and good stability after four cycle experiments. This study has provided a new viewpoint to design or develop the high-efficient photocatalysts for hydrogen production.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151114DOI Listing
October 2021

PbS Quantum Dots Saturable Absorber for Dual-Wavelength Solitons Generation.

Authors:
Ling Yun Wei Zhao

Nanomaterials (Basel) 2021 Sep 29;11(10). Epub 2021 Sep 29.

State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

PbS quantum dots (QDs), a representative zero-dimensional material, have attracted great interest due to their unique optical, electronic, and chemical characteristics. Compared to one- and two-dimensional materials, PbS QDs possess strong absorption and an adjustable bandgap, which are particularly fascinating in near-infrared applications. Here, fiber-based PbS QDs as a saturable absorber (SA) are studied for dual-wavelength ultrafast pulses generation for the first time to our knowledge. By introducing PbS QDs SA into an erbium-doped fiber laser, the laser can simultaneously generate dual-wavelength conventional solitons with central wavelengths of 1532 and 1559 nm and 3 dB bandwidths of 2.8 and 2.5 nm, respectively. The results show that PbS QDs as broadband SAs have potential application prospects for the generation of ultrafast lasers.
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http://dx.doi.org/10.3390/nano11102561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539987PMC
September 2021

Risk factors for myocardial injury at admission of 325 patients with coronavirus disease 2019 in Shanghai, China.

Australas J Ageing 2021 Oct 20. Epub 2021 Oct 20.

Department of Infection and Immunology, Shanghai Public Health Clinical Centre, Fudan University, Shanghai, China.

Objective: Myocardial injury leads to higher mortality in COVID-19, but the causes and risk factors are variable. We evaluated the potential risk factors for myocardial injury in COVID-19 patients to improve treatment strategies and reduce mortality.

Methods: This retrospective analysis enrolled 325 COVID-19 patients in Shanghai, China.

Results: The median age in our cohort was 51 [range 15-88] years, 26 (8%) were critically ill, and 177 patients (19.7%) had myocardial injury. The myocardial injury group comprised older, more critically ill patients with hypertension, other comorbidities, history of angiotensin-converting enzyme inhibitor/angiotensin receptor blocker use, lower peripheral blood lymphocyte count and higher D-dimer levels. Binary logistic regression analysis identified only age was an independent risk factor for myocardial injury (odds ratio 1.019; 95% confidence interval 1.003-1.036; age increase by 1 year = myocardial injury risk increase by 1.9%).

Conclusion: Older age was associated with a higher incidence of myocardial injury for COVID-19 patients.
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http://dx.doi.org/10.1111/ajag.13003DOI Listing
October 2021

Development of anti-breast cancer PI3K inhibitors based on 7-azaindole derivatives through scaffold hopping: Design, synthesis and in vitro biological evaluation.

Bioorg Chem 2021 Dec 6;117:105405. Epub 2021 Oct 6.

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, China. Electronic address:

Breast cancer is the cancer with the highest incidence all over the world. Phosphatidylinositol 3-kinase is an important regulator of intracellular signaling pathways, which is frequently mutated and overexpressed in majority of human breast cancers, and the inhibition of PI3K has been considered as a promising approach for the treatment of the cancer. Here, we report our design and synthesis of new 7-azaindole derivatives as PI3K inhibitors through the scaffold hopping strategy. By varying the groups at the 3-position of 7-azaindole, we identified a series of potent PI3K inhibitors, whose antiproliferative activities against two human breast cancer MCF-7 and MDA-MB-231 cell lines were evaluated. Representative derivatives FD2054 and FD2078 showed better activity than BKM120 in antiproliferation, reduced the levels of phospho-AKT and induced cell apoptosis. All these results suggested that FD2054 and FD2078 are potent PI3K inhibitors that could be considered as potential candidates for the development of anticancer agents.
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http://dx.doi.org/10.1016/j.bioorg.2021.105405DOI Listing
December 2021

A novel and comprehensive strategy for quality control in complex Chinese medicine formula using UHPLC-Q-Orbitrap HRMS and UHPLC-MS/MS combined with network pharmacology analysis: Take Tangshen formula as an example.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Oct 2;1183:122889. Epub 2021 Aug 2.

Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases, Department of Nephrology, China-Japan Friendship Hospital Beijing 100029, China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730, China. Electronic address:

The quality control of Chinese herbal medicines (CHM) is a key concern on the modernization and globalization. However, it is still a difficult task due to its multi-component, multi-target, multi-pathways. This study aims to provide a novel and comprehensive strategy for quality control in complex Chinese medicines (CHM) formulas by UHPLC-Q-Orbitrap HRMS and UHPLC-MS/MS combined with network pharmacology analysis. Tangshen formula (TSF) was used as an example for complex CHM formulas. The UHPLC-Q-Orbitrap HRMS was firstly applied to identify or tentatively assign 85 compounds in TSF. Subsequently, key active compounds for TSF treating diabetic nephropathy (DN) were chose by chemical-target-pathways network in network pharmacology. The results showed that 13 key bioactive compounds against DN including naringin, daidzein, genistein, formononetin, chlorogenic acid, aloe-emodin, nobiletin, tangeritin, ginsenoside Rg1, hesperetin, hesperidin, rhein, and limonin with three high topological features in chemical-target-pathways network were selected as Q-markers for quality control of TSF. Finally, the UHPLC-MS/MS was performed to simultaneously determine the concentrations of 13 Q-markers. And their concentrations were ranged from 11.57 to 3 788 µg·g. It suggested that many key bioactive compounds not only have high contents but also have wide range contents for the quality of complex CHM formulas. This study should be helpful to guide the selection of the Q-markers and provide new strategy for quality control of complex CHM formulas.
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http://dx.doi.org/10.1016/j.jchromb.2021.122889DOI Listing
October 2021

Extravasation of chemotherapeutic drug from an implantable intravenous infusion port in a child: A case report.

World J Clin Cases 2021 Sep;9(26):7840-7844

Department of Surgical Oncology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310052, Zhejiang Province, China.

Background: Drug extravasation is a complication of totally implantable access port (TIAP) use and could cause tissue injury and sustained organ dysfunction. Therefore, the clinical management of children with TIAP is challenging.

Case Summary: This was a case of extravasation of a chemotherapeutic drug (paclitaxel) from an implantable infusion port in a 23-mo old child. After fully evaluating the skin at the site of extravasation, the nurse continued to use the infusion port to complete the follow-up chemotherapeutic course. The skin around the infusion port was red, and showed no ulceration, swelling, or induration at discharge.

Conclusion: Since children are more active and often noncompliant, it is necessary to appropriately train pediatric nurses caring for individuals with TIAPs, and any abnormal situation should be timely addressed.
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http://dx.doi.org/10.12998/wjcc.v9.i26.7840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462233PMC
September 2021

Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals.

Cell Discov 2021 Sep 28;7(1):89. Epub 2021 Sep 28.

Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

SARS-CoV-2 outbreak has been declared by World Health Organization as a worldwide pandemic. However, there are many unknowns about the antigen-specific T-cell-mediated immune responses to SARS-CoV-2 infection. Here, we present both single-cell TCR-seq and RNA-seq to analyze the dynamics of TCR repertoire and immune metabolic functions of blood T cells collected from recently discharged COVID-19 patients. We found that while the diversity of TCR repertoire was increased in discharged patients, it returned to basal level ~1 week after becoming virus-free. The dynamics of T cell repertoire correlated with a profound shift of gene signatures from antiviral response to metabolism adaptation. We also demonstrated that the top expanded T cell clones (~10% of total T cells) display the key anti-viral features in CD8 T cells, confirming a critical role of antigen-specific T cells in fighting against SARS-CoV-2. Our work provides a basis for further analysis of adaptive immunity in COVID-19 patients, and also has implications in developing a T-cell-based vaccine for SARS-CoV-2.
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http://dx.doi.org/10.1038/s41421-021-00321-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476510PMC
September 2021

MiR-92b-3p Inhibits Proliferation of HER2-Positive Breast Cancer Cell by Targeting circCDYL.

Front Cell Dev Biol 2021 29;9:707049. Epub 2021 Jul 29.

Breast Tumor Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Objectives: Circular RNA (circRNA) is a novel class of RNA, which exhibits powerful biological function in regulating cellular fate of various tumors. Previously, we had demonstrated that over-expression of circRNA circCDYL promoted progression of HER2-negative (HER2) breast cancer miR-1275-ULK1/ATG7-autophagic axis. However, the role of circCDYL in HER2-positive (HER2) breast cancer, in particular its role in modulating cell proliferation, one of the most important characteristics of cellular fate, is unclear.

Materials And Methods: qRT-PCR and hybridization analyses were performed to examine the expression of circCDYL and miR-92b-3p in breast cancer tissues or cell lines. The biological function of circCDYL and miR-92b-3p were assessed by plate colony formation and cell viability assays and orthotopic animal models. In mechanistic study, circRNAs pull-down, RNA immunoprecipitation, dual luciferase report, western blot, immunohistochemical and immunofluorescence staining assays were performed.

Results: CircCDYL was high-expressed in HER2 breast cancer tissue, similar with that in HER2 breast cancer tissue. Silencing HER2 gene had no effect on expression of circCDYL in HER2 breast cancer cells. Over-expression of circCDYL promoted proliferation of HER2 breast cancer cells but not through miR-1275-ULK1/ATG7-autophagic axis. CircRNA pull down and miRNA deep-sequencing demonstrated the binding of miR-92b-3p and circCDYL. Interestingly, circCDYL did not act as miR-92b-3p sponge, but was degraded in miR-92b-3p-dependent silencing manner. Clinically, expression of circCDYL and miR-92b-3p was associated with clinical outcome of HER2 breast cancer patients.

Conclusion: MiR-92b-3p-dependent cleavage of circCDYL was an essential mechanism in regulating cell proliferation of HER2 breast cancer cells. CircCDYL was proved to be a potential therapeutic target for HER2 breast cancer, and both circCDYL and miR-92b-3p might be potential biomarkers in predicting clinical outcome of HER2 breast cancer patients.
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http://dx.doi.org/10.3389/fcell.2021.707049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358302PMC
July 2021

Analysis of risk factors of PICC-related bloodstream infection in newborns: implications for nursing care.

Eur J Med Res 2021 Jul 23;26(1):80. Epub 2021 Jul 23.

Department of Nursing, Children's Hospital, School of Medicine, Zhejiang University, No. 3333 Binsheng Road, Binjiang District, Hangzhou City, Zhejiang Province, China.

Background: It is necessary to analyze the characteristics and risk factors of catheter-related bloodstream infection (CRBSI) in newborns with peripherally inserted central catheter (PICC).

Methods: Newborns undergoing PICC catheterization in the neonatal department of our hospital from January 1, 2020 to January 31, 2021 were included. The characteristics of newborns with and without CRBSI newborns were compared and analyzed. Logistic regression analyses were performed to evaluate the risk factors of CRBSI in newborns with PICC.

Results: Three hundred eighty-six newborns with PICC were included, of whom 41 newborns had the CRBSI, the incidence of CRBSI in newborns with PICC was 10.62%. There were significant differences regarding the birth weight, durations of PICC stay, 5-min Apgar score, site of PICC insertion of PICC between CRBSI and no CRBSI group (all P < 0.05), and there were no significant differences regarding the gender, gestational age, cesarean section, mechanical ventilation and length of hospital stay between CRBSI and no CRBSI group (all P > 0.05). Escherichia coli (26.08%) and Staphylococcus aureus (23.92%) were the most common CRBSI pathogens in newborns with PICC. Logistic regression analysis indicated that birth weight ≤ 1500 g (OR 1.923, 95% CI 1.135-2.629), durations of PICC stay ≥ 21 days (OR 2.077, 95% CI 1.024-3.431), 5-min Apgar score ≤ 7 (OR 2.198, 95% CI 1.135-3.414) and femoral vein insertion of PICC (OR 3.044, 95% CI 1.989-4.306) were the independent risk factors of CRBSI in neonates with PICC (all P < 0.05).

Conclusion: For newborns with low birth weight, longer durations of PICC stay and femoral vein PICC insertion, they may have higher risks of CRBSI, and medical staff should take targeted measures to reduce the development of CRBSI.
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http://dx.doi.org/10.1186/s40001-021-00546-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299687PMC
July 2021

Discovery of cinnoline derivatives as potent PI3K inhibitors with antiproliferative activity.

Bioorg Med Chem Lett 2021 09 17;48:128271. Epub 2021 Jul 17.

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai, China. Electronic address:

Cinnoline is a potential pharmacophore which has rarely been reported for uses as PI3K inhibitors. In this study, a series of cinnoline derivatives were developed as PI3K inhibitors and evaluated for enzymatic and cellular activities. Most compounds displayed nanomolar inhibitory activities against PI3Ks, among which 25 displayed high LLE and micromolar inhibitory potency against three human tumor cell lines (IC = 0.264 μM, 2.04 μM, 1.14 μM).
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http://dx.doi.org/10.1016/j.bmcl.2021.128271DOI Listing
September 2021

Paper-based wearable electronics.

iScience 2021 Jul 17;24(7):102736. Epub 2021 Jun 17.

Department of Biomedical, Biological & Chemical Engineering, University of Missouri, Columbia, MO 65211, USA.

Skin-interfaced wearable electronics can find a broad spectrum of applications in healthcare, human-machine interface, robotics, and others. The state-of-the-art wearable electronics usually suffer from costly and complex fabrication procedures and nonbiodegradable polymer substrates. Paper, comprising entangled micro- or nano-scale cellulose fibers, is compatible with scalable fabrication techniques and emerges as a sustainable, inexpensive, disposable, and biocompatible substrate for wearable electronics. Given various attractive properties (e.g., breathability, flexibility, biocompatibility, and biodegradability) and rich tunability of surface chemistry and porous structures, paper offers many exciting opportunities for wearable electronics. In this review, we first introduce the intriguing properties of paper-based wearable electronics and strategies for cellulose modifications to satisfy specific demands. We then overview the applications of paper-based devices in biosensing, energy storage and generation, optoelectronics, soft actuators, and several others. Finally, we discuss some challenges that need to be addressed before practical uses and wide implementation of paper-based wearable electronics.
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http://dx.doi.org/10.1016/j.isci.2021.102736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261674PMC
July 2021

Bioisosteric replacements of the indole moiety for the development of a potent and selective PI3Kδ inhibitor: Design, synthesis and biological evaluation.

Eur J Med Chem 2021 Nov 21;223:113661. Epub 2021 Jun 21.

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai, 200433, China. Electronic address:

Based on indole scaffold, a potent and selective phosphoinositide 3-kinase delta (PI3Kδ) inhibitor, namely FD223, was developed by the bioisosteric replacement drug discovery approach and studied for the treatment of acute myeloid leukemia (AML). In vitro studies revealed that FD223 displays high potency (IC = 1 nM) and selectivity (29-51 fold over other PI3K isoforms) against PI3Kδ, and exhibits efficient inhibition of the proliferation of AML cell lines (MOLM-16, HL-60, EOL-1 and KG-1) by suppressing p-AKT Ser473 thus causing G1 phase arrest during the cell cycle. Further given the favorable pharmacokinetic (PK) profiles of FD223, in vivo studies were evaluated using xenograft model in nude mice, confirming its significant antitumor efficacy meanwhile with no observable toxicity. All these results are comparable to the positive group of Idelalisib (CAL-101), indicating that FD223 has potential for further development as a promising PI3Kδ inhibitor for the treatment of leukemia such as AML.
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http://dx.doi.org/10.1016/j.ejmech.2021.113661DOI Listing
November 2021

[Simultaneous determination of eight additives in polyethylene food contact materials by ultrahigh-performance liquid chromatography].

Se Pu 2021 May;39(5):488-493

Chinese Academy of Inspection and Quarantine, Beijing 100176, China.

Measurement of additive residues in food contact materials is important for safety monitoring at the initial stage. Most of the current studies focus on the determination of the migration amounts of chemical hazards from food contact materials into food simulants. Studies on chemical hazard residues in food contact materials are limited to monomers, oligomers, heavy metals, phthalic acid esters, and biphenols, which are known environmental pollutants. Only a few studies have investigated analysis methods for additive residues in food contact materials. In this study, the main factors (monitoring wavelength, chromatographic column, mobile phase, extraction solvent, etc.) that affect the accuracy and sensitivity of eight compounds, including three antioxidants, three light stabilizers, and two plasticizers, were investigated during sample preparation and instrument analysis. A method based on ultrahigh-performance liquid chromatography (UPLC) was developed for the simultaneous determination of these eight additives in polyethylene (PE). The PE food contact material sample was ground to homogenize the particle sizes under freeze-grinding. After comparing the extraction efficiencies of methylbenzene, chloroform, acetone, and acetonitrile, 2.0 g of the sample was extracted with methylbenzene at 80 ℃ and 10.34-11.72 MPa (1500-1700 psi) by accelerated solvent extraction (ASE) for 10 min once. The exaction solvent (10 mL) was transferred and concentrated to near dryness under a gentle stream of nitrogen gas and then re-dissolved in 10 mL of the initial mobile phase (70% (v/v) methanol in water). Finally, the eight compounds were analyzed by UPLC. After optimization of the analytical column and mobile phases, the eight analytes were separated on an ACQUITY UPLC BEH C chromatographic column (100 mm×2.1 mm, 1.7 μm) by gradient elution using water and acetonitrile as the mobile phases. The column oven temperature, flow rate of the mobile phase, and injection volume were 30 ℃, 0.3 mL/min, and 5 μL, respectively. The analytes were detected by a diode assay detector (DAD) in the scanning range of 210 nm to 400 nm. The monitoring wavelength was set at 230 nm, 250 nm, 280 nm, and 330 nm. External standard calibration curves were used for quantification. Under the optimized conditions, the calibration curves for the eight compounds showed good linearity in the range of 0.2 μg/mL to 10 μg/mL, and the correlation coefficients were >0.999. The recoveries in spiked blank polyethylene samples at the level of 0.05% were in the range of 83.8% to 103.4%, with relative standard deviations (RSDs) ranging from 0.14% to 7.86%. To validate the method, PE reference materials containing these eight compounds were manufactured at the content level of 0.2% to 0.9%. The recoveries using the prepared reference materials ranged from 63.5% to 118.5%, and the RSDs were in the range of 4.61% to 15.6%. The limits of detection (LODs, =3) of all the eight compounds were 0.005% and the limits of quantification (LOQs, =10) were 0.02%, in compliance with the current legislation. To assess the feasibility and potential of the proposed approach for routine analyses of these eight compounds, the developed method was applied to the analysis of these compounds in ten PE food packages and PE gloves. In six samples, tris(2,4-di--butylphenyl)phosphite (Irganox 168) was detected at a level of 0.02%-0.07%, which was lower than the maximum level of this compound in PE food contact material products regulated in GB 9685-2016 at 0.2%. The method is compliant with the current legislation, and it can be used for the monitoring and supervision of these eight additives in PE food contact materials.
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http://dx.doi.org/10.3724/SP.J.1123.2020.12002DOI Listing
May 2021

[Determination of 15 lipid regulators in fish meat by QuEChERS-ultra performance liquid chromatography-quadrupole/ electrostatic field orbitrap mass spectrometry].

Se Pu 2020 Jun;38(6):655-662

Institute of Food Safety, Chinese Academy of Inspection&Quarantine, Beijing 100176, China.

An analytical method was established for the determination of 15 lipid regulators in fish meat by QuEChERS combined with ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry (UPLC-Q/Orbitrap-MS). The samples were purified by optimized QuEChERS methods. The amounts of the adsorbent materials (primary secondary amine (PSA) (20, 60, 100, 140 and 180 mg), C18 (40, 100, 160, 220 and 280 mg) and sodium acetate (0.2, 0.6, 1.0, 1.4 and 1.8 g)) were optimized by the response surface method to obtain the best purification effect. The target compounds were separated on an XBridge-C18 column (100 mm×2.1 mm, 3.5 μm) using acetonitrile-0.1% (v/v) formic acid aqueous solution (containing 1.5 mmol/L ammonium acetate) as the mobile phases by a gradient elution program. Qualitative and quantitative analysis of the target compounds were performed in the full scan and secondary mass spectrometry scan (dd-MS) modes with positive and negative ionization. The target compounds showed good linear relationships in their respective ranges, with correlation coefficients () greater than 0.99. The limits of detection (LOD, =3) and limits of quantification (LOQ, =10) were in the range of 0.2-1.0 μg/kg and 0.3-3.1 μg/kg, respectively. The average recoveries were 76.4%-116.0% at LOQ, 2-fold LOQ, and 10-fold LOQ levels. The intra-day relative standard deviations (RSDs) were 1.0%-7.9%, and the inter-day RSDs were 1.7%-18.4%. The method is simple, sensitive and accurate, and it is suitable for the determination and quantification of lipid regulators in fish meat.
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http://dx.doi.org/10.3724/SP.J.1123.2019.10011DOI Listing
June 2020

miR-320a in serum exosomes promotes myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells.

Exp Ther Med 2021 Aug 14;22(2):873. Epub 2021 Jun 14.

Department of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, P.R. China.

MicroRNAs (miRNAs/miRs) serve an important role in the pathogenesis of chronic heart failure (CHF). A number of reports have illustrated the regulatory effect of serum exosomal miRNA on myocardial fibrosis. The present study aimed to investigate the expression of miR-320a in serum exosomes, as well as the effect of miR-320a on myocardial fibroblast proliferation. Serum exosome samples from 10 patients with CHF and 5 healthy volunteers were obtained and characterized. mRNA and protein expression levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. The content of soluble growth stimulation expressed gene 2 (sST2) was determined via ELISA. HEH2 cell viability and apoptosis were detected by performing MTT assays and flow cytometry, respectively. The results demonstrated that serum miR-320a expression levels and sST2 content were significantly increased in patients with CHF compared with healthy controls, and the expression of serum miR-320a was significantly correlated with clinical CHF indexes. miR-320a expression levels were significantly increased in exosomes isolated from patients with CHF compared with those isolated from healthy controls. Phosphoinositide-3-kinase catalytic α polypeptide gene (PIK3CA) expression levels and sST2 content were increased in HEH2 cells following transfection with miR-320a mimics compared with NC-mimic, whereas miR-320a inhibitor displayed contrasting effects by reduced the cell viability and apoptosis in myocardial fibroblasts compared with the NC-inhibitor group. The protein expression levels of collagen I, collagen III, α-smooth muscle actin, phosphorylated (p)-mTOR (ser 2448)/mTOR, p-Akt (ser 473)/Akt, p-Akt (thr 308)/Akt and PIK3CA were significantly increased in miR-320a mimic-transfected HEH2 cells compared with the NC-mimics groups. By contrast, miR-320a inhibitor notably downregulated the expression levels of these proteins compared with the NC-inhibitor group. Collectively, the results of the present study demonstrated that miR-320a promoted myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells, suggesting that serum exosomal miR-320a may serve as a potential biomarker for the diagnosis of CHF.
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http://dx.doi.org/10.3892/etm.2021.10305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237386PMC
August 2021

High PD-L1 expression on immune cells, but not on tumor cells, is a favorable prognostic factor in urothelial carcinoma.

Future Oncol 2021 Aug 30;17(22):2893-2905. Epub 2021 Jun 30.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100021, China.

To explore the prognostic value of high PD-L1 expression on tumor cells (TC) and tumor-infiltrating immune cells (TIIC) in urothelial carcinoma (UC). 162 UC specimens were evaluated for PD-L1 expression on TIIC and TC with the SP263 assay. High PD-L1 expression was defined as ≥25% staining. High PD-L1 expression on TC in UC patients with stage T1-4 disease was associated with poor overall survival. However, high PD-L1 expression on TIIC in UC patients with stage T1-4 disease revealed favorable disease-free and overall survival; more significant differences were observed in patients with stages T2-4. Multivariate analysis revealed that high PD-L1 expression on TIIC was an independent prognostic predictor for better disease-free and overall survival. High PD-L1 expression on TIIC, but not on TC, is a favorable prognostic factor in UC.
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http://dx.doi.org/10.2217/fon-2021-0092DOI Listing
August 2021

Hollow carbon nanospheres dotted with Gd-Fe nanoparticles for magnetic resonance and photoacoustic imaging.

Nanoscale 2021 Jun 16;13(24):10943-10952. Epub 2021 Jun 16.

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai, 200433, China.

Integrating magnetic resonance (MR) and photoacoustic (PA) contrast agents into porous nanomaterials is a favorable way for screening of potential theranostic nanomedicines. Hollow carbon nanospheres (HCSs) dotted with GdPO and γ-FeO (Gd-Fe) nanoparticles are therefore prepared and studied in this work. The resultant Gd-Fe/HCSs possess a size of ∼100 nm with a cavity of ∼80 nm and a shell thickness of ∼10 nm, where the magnetic Gd-Fe nanoparticles are dotted. Owing to the synergistic effects, the Gd-Fe/HCSs give 2.5 times enhanced PA signals as compared with HCSs as well as the inherited MR imaging properties from Gd-Fe nanoparticles. In vivo MR and PA imaging of the liver in mice are consequently evaluated and validated. Furthermore, taking the tunable particle size, hollow cavity, shell thickness, and dotted amounts of nanoparticles into consideration, our studies here provide a useful structural model for the synergistic integration of MR and PA imaging in HCSs.
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http://dx.doi.org/10.1039/d1nr02914bDOI Listing
June 2021

Integrating longitudinal clinical laboratory tests with targeted proteomic and transcriptomic analyses reveal the landscape of host responses in COVID-19.

Cell Discov 2021 Jun 8;7(1):42. Epub 2021 Jun 8.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The pathophysiology of coronavirus disease 19 (COVID-19) involves a multitude of host responses, yet how they unfold during the course of disease progression remains unclear. Here, through integrative analysis of clinical laboratory tests, targeted proteomes, and transcriptomes of 963 patients in Shanghai, we delineate the dynamics of multiple circulatory factors within the first 30 days post-illness onset and during convalescence. We show that hypercortisolemia represents one of the probable causes of acute lymphocytopenia at the onset of severe/critical conditions. Comparison of the transcriptomes of the bronchoalveolar microenvironment and peripheral blood indicates alveolar macrophages, alveolar epithelial cells, and monocytes in lungs as the potential main sources of elevated cytokines mediating systemic immune responses and organ damages. In addition, the transcriptomes of patient blood cells are characterized by distinct gene regulatory networks and alternative splicing events. Our study provides a panorama of the host responses in COVID-19, which may serve as the basis for developing further diagnostics and therapy.
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http://dx.doi.org/10.1038/s41421-021-00274-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185699PMC
June 2021

Evaluation of superinfection, antimicrobial usage, and airway microbiome with metagenomic sequencing in COVID-19 patients: A cohort study in Shanghai.

J Microbiol Immunol Infect 2021 Oct 6;54(5):808-815. Epub 2021 Apr 6.

Departments of Infectious Diseases, Zhongshan Hospital of Fudan University, People's Republic of China. Electronic address:

Background: In COVID-19 patients, information regarding superinfection, antimicrobial assessment, and the value of metagenomic sequencing (MS) could help develop antimicrobial stewardship.

Method: This retrospective study analyzed 323 laboratory-confirmed COVID-19 patients for co-infection rate and antimicrobial usage in the Shanghai Public Health Clinical Center (SPHCC) from January 23rd to March 14th 2020. The microbiota composition was also investigated in patients with critically severe COVID-19.

Results: The total population co-infection rate was 17/323 (5.3%) and 0/229 (0), 4/78 (5.1%), and 13/16 (81.3%) for the mild, severe, and critically severe subgroups, respectively. Proven fungal infection was significantly associated with a higher mortality rate (p = 0.029). In critically severe patients, the rate of antimicrobials and carbapenem usage were 16/16 (100%) and 13/16 (81.3%), respectively, in which the preemptive and empiric antimicrobial days accounted for 51.6% and 30.1%, respectively. Targeted therapy only accounted for 18.3%. MS was implemented to detect non-COVID-19 virus co-existence and the semi-quantitative surveillance of bacteremia, with clear clinical benefit seen in cases with MS-based precision antimicrobial management. Airway microbiome analysis suggested that the microbiota compositions in critically severe COVID-19 patients were likely due to intubation and mechanical ventilation.

Conclusions: In the SPHCC cohort, we observed a non-negligible rate of super-infection, especially for the critically ill COVID-19 patients. Fungal co-infection requires intensive attention due to the high risk of mortality, and the clinical benefit of MS in guiding antimicrobial management warrants further investigation.
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http://dx.doi.org/10.1016/j.jmii.2021.03.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021444PMC
October 2021

A novel treatment regimen of granulocyte colony-stimulating factor combined with ultra-low-dose decitabine and low-dose cytarabine in older patients with acute myeloid leukemia and myelodysplastic syndromes.

Ther Adv Hematol 2021 30;12:20406207211009334. Epub 2021 Apr 30.

Department of Hematology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou, Juqian Road 185, Changzhou, Jiangsu 213000, China.

Background: Older patients with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) unfit for intensive chemotherapy are emergent for suitable treatment strategies. Hypomethylating agents and low-dose cytarabine have generated relevant benefits in the hematological malignancies over recent decades. We evaluated the efficacy and safety of the novel treatment regimen consisting of ultra-low-dose decitabine and low-dose cytarabine, with granulocyte colony-stimulating factor (G-CSF) in this population of patients.

Methods And Materials: Patients aged more than 60 years with newly diagnosed AML/MDS were enrolled to receive therapy combined of 300 µg subcutaneously per day for priming, decitabine 5.15-7.62 mg/m/d intravenously and cytarabine 15 mg/m/d twice a day subcutaneously and G-CSF for consecutive 10 days every 28 days. The study enrolled 28 patients unfit for standard intensive chemotherapy. The median age of patients was 68 years (range 60-83 years) and 20 (71.4%) patients harbored AML. The primary outcome was to evaluate overall response rate.

Results: Overall, this novel ultra-low-dose treatment regimen was well tolerated, with 0% of both 4- and 8-week mortality occurrence. Objective response rate (CR + CRi + PR in AML and CR + mCR + PR in MDS) was 57.1% after the first treatment course. Responses of hematologic improvement (HI) aspect were achieved in 18 of 28 (64.3%) patients, 11 (39.3%), 12 (42.9%), and eight patients (28.6%) achieved HI-E, HI-P, HI-N, respectively.

Conclusions: Untreated elderly with AML/MDS were well tolerated and benefited from this novel ultra-low-dose treatment regimen.
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http://dx.doi.org/10.1177/20406207211009334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111530PMC
April 2021

Salvianolic acid A alleviated inflammatory response mediated by microglia through inhibiting the activation of TLR2/4 in acute cerebral ischemia-reperfusion.

Phytomedicine 2021 Jul 15;87:153569. Epub 2021 Apr 15.

Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, People's Republic of China; Department of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, People's Republic of China. Electronic address:

Background: Toll-like receptor 2 and Toll-like receptor 4 (TLR2/4) on microglia have been found as important regulators in the inflammatory response during cerebral ischemia/reperfusion (I/R). In China, traditional Chinese medicine Salvia miltiorrhiza (danshen) and its some components are considered to be effective in rescuing cerebral I/R injury through clinical practice.

Hypothesis/purpose: Here we examined the effect of Salvianolic acid A (SAA), a monomer compound in the water extract of Salvia miltiorrhiza, on TLR2/4 of microglia and its mediated inflammatory injury during cerebral I/R in vivo and in vitro.

Study Design: For exploring the effect of SAA on cerebral I/R and TLR2/4, classic middle cerebral artery occlusion (MCAO) model and oxygen glucose deprivation / reoxygenation (OGD/R) model of co-culture with primary hippocampal neurons and microglia in vitro were used. Signal pathway research and gene knockout have been applied to further explain its mechanism.

Methods: The evaluation indexes of I/R injury included infarct size, edema degree and pathology as well as primary hippocampal neurons and microglia culture, ELISA, western, RT-PCR, HE staining, immunofluorescence, flow cytometry, siRNA gene knockout were also employed.

Results: SAA significantly improved the degree of brain edema and ischemic area in I/R rats accompanied by decreases in levels of interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α). Pathological staining revealed that SAA could reduce inflammatory cell infiltration and mcirogila activation after reperfusion. Both protein and gene expression of TLR2 and TLR4 in ischemic hemisphere were obviously inhibited by SAA treatment while changes were not found in the non-ischemic hemisphere. In order to further study its mechanism, OGD/R model was used to mimic inflammatory damage of ischemic tissue by co-culturing primary rat hippocampal neurons and microglial cells. It was found that SAA also inhibited the protein and gene expression of TLR2 and TLR4 after OGD/R injury in microglia. After TLR2/4 knockout, the inhibitory effect of SAA on IL-1β and TNF-α levels in cell supernatant and neuron apoptosis were significantly weakened in each dose group. Moreover, expression levels of myeloid differentiation factor 88 (MyD88), NFκB, IL-1β and IL-6 in TLR2/4 mediated inflammatory pathway were reduced with SAA treatment.

Conclusion: SAA could significantly reduce the inflammatory response and injury in cerebral ischemia-reperfusion in vivo and in vitro, and its mechanism may be through the inhibition of TLR2/4 and its related signal pathway.
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http://dx.doi.org/10.1016/j.phymed.2021.153569DOI Listing
July 2021

High Dose Intravenous Vitamin C for Preventing The Disease Aggravation of Moderate COVID-19 Pneumonia. A Retrospective Propensity Matched Before-After Study.

Front Pharmacol 2021 22;12:638556. Epub 2021 Apr 22.

Department of Respiratory and Critical Care Medicine of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Coronavirus disease 2019 (COVID-19) pandemic is continuing to impact multiple countries worldwide and effective treatment options are still being developed. In this study, we investigate the potential of high-dose intravenous vitamin C (HDIVC) in the prevention of moderate COVID-19 disease aggravation. In this retrospective before-after case-matched clinical study, we compare the outcome and clinical courses of patients with moderate COVID-19 patients who were treated with an HDIVC protocol (intravenous injection of vitamin C, 100 mg/kg/day, 1 g/h, for 7 days from admission) during a one-month period (between March 18 and april 18, 2020, HDIVC group) with a control group treated without the HDIVC protocol during the preceding two months (January 18 to March 18, 2020). Patients in the two groups were matched in a 1:1 ratio according to age and gender. The HDIVC and control groups each comprised 55 patients. For the primary outcomes, there was a significant difference in the number of patients that evolved from moderate to severe type between the two groups (HDIVC: 4/55 vs. control: 12/55, relative risk [RR] = 0.28 [0.08, 0.93], = 0.03). Compared to the control group, there was a shorter duration of systemic inflammatory response syndrome (SIRS) ( = 0.0004) during the first week and lower SIRS occurrence (2/21 vs 10/22, = 0.0086) on Day 7 (6-7 days after admission). In addition, HDIVC group had lower C-reactive protein levels ( = 0.005) and higher number of CD4 T cells from Day 0 (on admission) to Day 7 ( = 0.04)." The levels of coagulation indicators, including activated partial thromboplastin time and D-dimer were also improved in the HDIVC compared to the control group on Day 7. HDIVC may be beneficial in limiting disease aggravation in the early stage of COVID-19 pneumonia, which may be related to its improvements on the inflammatory response, immune function and coagulation function. Further randomized controlled trials are required to augment these findings.
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http://dx.doi.org/10.3389/fphar.2021.638556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100592PMC
April 2021

Multimetal lanthanide phosphonocarboxylate frameworks: structures, colour tuning and near-infrared emission.

Dalton Trans 2021 Jun;50(21):7380-7387

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200438, China.

A series of isostructural lanthanide phosphonocarboxylate frameworks {(H3O)3[Ln7(pbpdc)6(DMF)4(H2O)3]·4H2O}n (named LnPCF, Ln = Tb, Eu and Gd, H4pbpdc = 4'-phosphono-[1,1'-biphenyl]-3,5-dicarboxylic acid) were solvothermally synthesized and characterized by the single crystal X-ray diffraction technique. By combining lanthanide cations with a phosphonocarboxylate ligand, a heptametallic lanthanide phosphonate [Ln7(PO3)6(COO)12] core was obtained. This core exhibited as a rare highly 18-connected node and was linked by the 3-connected pbpdc4- ligand, forming a (3,18)-connected framework with a novel topology of {43}6{438·676·839}. This LnPCF structure is an ideal platform for accommodating various lanthanide ions. The TbPCF and EuPCF show efficient luminescence emission due to the "antenna effect" and incorporating Gd3+ into the TbPCF results in a drastic luminescence enhancement. Fine colour tuning between green and red can be easily achieved in bimetallic TbxGd1-xPCFs. More significantly, upon combining a few percent of Nd3+ and Gd3+ with Tb3+, the resulting trimetallic Tb0.4Gd0.5Nd0.1PCF shows dual emissions of both visible and near-infrared light.
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http://dx.doi.org/10.1039/d1dt01052bDOI Listing
June 2021

Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient.

Diagn Pathol 2021 May 5;16(1):40. Epub 2021 May 5.

Department of Infectious Disease, Shanghai Public Health Clinical center, Fudan University, Shanghai, China.

Aims: Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19.

Methods: The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid-Schiff) staining, Gram staining, Ziehl-Neelsen stain and Grocott's Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed.

Results: We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68 macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity.

Conclusions: The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection.
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http://dx.doi.org/10.1186/s13000-021-01082-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097675PMC
May 2021

Poria cocos polysaccharide attenuates damage of nervus in Alzheimer's disease rat model induced by D-galactose and aluminum trichloride.

Neuroreport 2021 05;32(8):727-737

Department of Febrile Disease, Basic Medicine College, Nanjing University of Chinese Medicine, Jiangsu, People's Republic of China.

Poria cocos polysaccharide (PCP) is a compound from Poria cocos, and which is used as a classical tonic agent. This article aims to investigate the effects of PCP on neuronal damage of hippocampus and cognitive function in a rat model of Alzheimer's disease induced by D-galactose and aluminum trichloride. Oxiracetam (ORC) was used as a positive drug in this experiment. The rats were treated with PCP at doses of 100, 200 and 300 mg/kg/day for 30 days and ORC at dose of 346 mg/kg/day after modeling. The results of behavioral test showed that PCP could prevent cognitive decline in Alzheimer's disease rats as assessed by Y-maze test and Morris water maze test. Results of hippocampus slices showed that neurons were integrated and regularly arranged in the groups, which were administered along with PCP. Moreover, PCP could reduce neuronal apoptosis in hippocampus of Alzheimer's disease rats. Furthermore, the activities of superoxide dismutase in the hippocampus were elevated by PCP administration, while acetyl cholinesterase, reactive oxygen, malondialdehyde and inflammatory factors levels were reduced. In addition, we found PCP could attenuate MAPK/NF-κB signal pathway in the hippocampus. All results illustrated that PCP could exert neuroprotective effects at least partly through alleviating oxidative stress, apoptosis, inflammation and inhibiting the MAPK/NF-κB pathway in Alzheimer's disease rats induced by D-galactose and aluminum trichloride.
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http://dx.doi.org/10.1097/WNR.0000000000001648DOI Listing
May 2021

Prediction of Freezing of Gait in Parkinson's Disease Using a Random Forest Model Based on an Orthogonal Experimental Design: A Pilot Study.

Front Hum Neurosci 2021 22;15:636414. Epub 2021 Mar 22.

Department of Neurology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.

Purpose: The purpose of this study was to introduce an orthogonal experimental design (OED) to improve the efficiency of building and optimizing models for freezing of gait (FOG) prediction.

Methods: A random forest (RF) model was developed to predict FOG by using acceleration signals and angular velocity signals to recognize possible precursor signs of FOG (preFOG). An OED was introduced to optimize the feature extraction parameters.

Results: The main effects and interaction among the feature extraction hyperparameters were analyzed. The false-positive rate, hit rate, and mean prediction time (MPT) were 27%, 68%, and 2.99 s, respectively.

Conclusion: The OED was an effective method for analyzing the main effects and interactions among the feature extraction parameters. It was also beneficial for optimizing the feature extraction parameters of the FOG prediction model.
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http://dx.doi.org/10.3389/fnhum.2021.636414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044955PMC
March 2021

Laser-induced graphene for bioelectronics and soft actuators.

Nano Res 2021 Apr 7:1-18. Epub 2021 Apr 7.

Department of Biomedical, Biological & Chemical Engineering, University of Missouri, Columbia, Missouri 65211 USA.

Laser-assisted process can enable facile, mask-free, large-area, inexpensive, customizable, and miniaturized patterning of laser-induced porous graphene (LIG) on versatile carbonaceous substrates (e.g., polymers, wood, food, textiles) in a programmed manner at ambient conditions. Together with high tailorability of its porosity, morphology, composition, and electrical conductivity, LIG can find wide applications in emerging bioelectronics (e.g., biophysical and biochemical sensing) and soft robots (e.g., soft actuators). In this review paper, we first introduce the methods to make LIG on various carbonaceous substrates and then discuss its electrical, mechanical, and antibacterial properties and biocompatibility that are critical for applications in bioelectronics and soft robots. Next, we overview the recent studies of LIG-based biophysical (e.g., strain, pressure, temperature, hydration, humidity, electrophysiological) sensors and biochemical (e.g., gases, electrolytes, metabolites, pathogens, nucleic acids, immunology) sensors. The applications of LIG in flexible energy generators and photodetectors are also introduced. In addition, LIG-enabled soft actuators that can respond to chemicals, electricity, and light stimulus are overviewed. Finally, we briefly discuss the future challenges and opportunities of LIG fabrications and applications.
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http://dx.doi.org/10.1007/s12274-021-3441-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023525PMC
April 2021
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