Publications by authors named "Yun Hu"

375 Publications

Bifunctional electrocatalysts for oxygen reduction and oxygen evolution: a theoretical study on 2D metallic WO-supported single atom (Fe, Co, or Ni) catalysts.

Phys Chem Chem Phys 2021 Jun 14. Epub 2021 Jun 14.

Department of Materials Science and Engineering, Michigan Technological University, Houghton, Michigan 49931, USA.

Catalysts play a critical role in the oxygen evolution reaction (OER) and the oxygen reduction reaction (ORR) for energy storage, conversion, and utilization. Herein, first-principles density functional theory (DFT) calculations demonstrated that single-metal-atom (Fe, Co, or Ni) sites can bind to the surface of 2D WO2, enhancing the adsorption of intermediates involved in the OER/ORR. Furthermore, it was found that the single-metal-atom-doped 2D WO2 achieves the smallest OER and ORR overpotentials of 0.42 V and 0.40 V, respectively, which are comparable to those of IrO2 or Pt-based catalysts. This predicts the excellent OER/ORR catalytic activities of the single-metal-atom (Fe, Co, or Ni) doped 2D WO2, which would be a promising bifunctional catalyst for fuel cells, water splitting, and metal-air batteries.
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http://dx.doi.org/10.1039/d1cp00540eDOI Listing
June 2021

Fe-based metal organic framework derivative with enhanced Lewis acidity and hierarchical pores for excellent adsorption of oxygenated volatile organic compounds.

Sci Total Environ 2021 May 29;790:148132. Epub 2021 May 29.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China; Guangdong Provincial Engineering and Technology Research Centre for Environmental Risk Prevention and Emergency Disposal, Guangzhou 510006, PR China. Electronic address:

A series of Fe-based metal organic framework derived materials were prepared by thermal treating MIL-100(Fe) in nitrogen atmosphere for adsorption removal of oxygenated volatile organic compounds (OVOCs) such as methanol, formaldehyde and acetone under dynamic conditions. The experimental results showed that the partially carbonized M-350 material obtained by calcining MIL-100(Fe) at 350 °C exhibited the best adsorption performance and high stability. The breakthrough adsorption capacity of M-350 for methanol was 61.5% higher than that of pure MIL-100 (Fe), and it was 24.7, 6.5 and 2.6 times higher than that of commercial activated carbon, ZSM-5 and SAPO-34 adsorbents, respectively. The excellent adsorption performance was attributed to the exposure of abundant coordinatively unsaturated iron metal sites acting as Lewis acid sites through high temperature calcination, which had a strong affinity for OVOCs. Meanwhile, a hierarchical porous structure and high specific surface area further promoted the adsorption. This work provides new insights into the further development of metal organic frameworks based functional materials for VOCs removal and purification.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148132DOI Listing
May 2021

Formulation of Nanovaccines toward an Extended Immunity against Nicotine.

ACS Appl Mater Interfaces 2021 Jun 9. Epub 2021 Jun 9.

Department of Biological Systems Engineering, Virginia Tech, Blacksburg, Virginia 24061, United States.

Nicotine vaccines have been investigated to assist with smoking cessation. Because smoking cessation is a long process, past nicotine vaccines required multiple injections to achieve long-term efficacy. It would be of great significance if extended efficacy can be achieved with fewer injections. Here, we report the assembly of lipid-polylactic acid (PLA) and lipid-poly(lactic--glycolic acid) (PLGA) hybrid nanoparticle (NP) based nicotine vaccines. Mice immunized with the lipid-PLGA vaccine produced higher titers of nicotine-specific antibodies than the lipid-PLA vaccine in short-term. However, the lipid-PLA vaccine was found to induce long-lasting antibodies. Three months after the immunization, only mice that received first two injections of the lipid-PLGA vaccine and a third injection of the lipid-PLA vaccine achieved a significantly lower brain nicotine concentration of 65.13 ± 20.59 ng/mg than 115.88 ± 37.62 ng/mg from the negative controls. The results indicate that not only the stability of the vaccines but also the combination of the vaccines impacted the long-term efficacy of the immunization. Lastly, both the body weight and the histopathology study suggest that the vaccines were safe to mice. These findings suggest that long-term immunity against nicotine can be realized by a rational administration of nanovaccines of different levels of stability.
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http://dx.doi.org/10.1021/acsami.1c07049DOI Listing
June 2021

Effectiveness of beinaglutide in a patient with late dumping syndrome after gastrectomy: A case report.

Medicine (Baltimore) 2021 May;100(21):e26086

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Jiangsu, China.

Rationale: Dumping syndrome is a frequent and potentially severe complication after gastric surgery. Beinaglutide, a recombinant human glucagon-like peptide-1 (GLP-1) which shares 100% homology with human GLP-1(7-36), has never been reported in the treatment of dumping syndrome before.

Patient Concerns: The patient had undergone distal gastrectomy for gastric signet ring cell carcinoma 16 months ago. He presented with symptoms of paroxysmal palpitation, sweating, and dizziness for 4 months.

Diagnosis: He was diagnosed with late dumping syndrome.

Interventions And Outcomes: The patient was treated with dietary changes and acarbose for 4 months before admitted to our hospital. The treatment with dietary changes and acarbose did not prevent postprandial hyperinsulinemia and hypoglycemia according to the 75 g oral glucose tolerance test (OGTT) and continuous glucose monitoring (CGM) on admission.Therefore, the patient was treated with beinaglutide 0.1 mg before breakfast and lunch instead of acarbose. After the treatment of beinaglutide for 1 month, OGTT showed a reduction in postprandial hyperinsulinemia compared with before starting treatment, and the time in the range of 3.9 to 10 mmol/L became 100% in CGM. No side effect was observed in this patient during beinaglutide treatment.

Lessons: These findings suggest that beinaglutide may be effective for treating post-gastrectomy late dumping syndrome.
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http://dx.doi.org/10.1097/MD.0000000000026086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154494PMC
May 2021

[1 Promoted the Proliferation and Migration Ability of BMSCs in Glioma C6 Microenvironment].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 May;52(3):438-444

The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing 401145, China.

Objective: To investigate the changes in the proliferation and migration ability of bone marrow mesenchymal stem cells (BMSCs) after indirect co-culturing with glioma C6 cells, and to examine the role of plasmacytoma variant translocation 1 gene ( 1), a long non-coding RNA (lncRNA), in these changes.

Methods: After separation, cultivation and identification of BMSCs, BMSCs of good growth condition were picked out and indirectly co-cultured with glioma C6 cells in Transwell chambers. These cells are henceforth referred to as the co-culture group. Normal BMSCs cultured separately were the control group. CCK-8 and soft agar colony formation assay were used to examine the proliferation ability of the two groups of cells. Flow cytometry was used to examine the cell cycle. Wound healing assay and Transwell assay were used to explore the migration ability of the cells. Quantitative real-time PCR (qRT-PCR) was used to examine the genetic expression level of 1 in the two groups. The above-mentioned tests were repeated after the co-cultured BMSCs were transfected with si- 1 (si- 1 group) and si-NC (si-NC group). In addition, qRT-PCR was done to evaluate the expression of CyclinD1, a cell cycle protein gene, and matrix metalloproteinases 2 and 9 ( 2 and 9), the migration-related genes in the si- 1 and si-NC transfected co-cultured BMSCs.

Results: The BMSCs used in the present study possess the capability of osteogeneic and adipogenic differentiation. Compared with the control group, the co-cultured BMSCs had smaller size, disorderly arrangement and the lack of intercellular contact inhibition. The proliferation and migration ability was significantly enhanced, the proportions of S and G phase cells greatly increased and the expression level of 1 was significantly up-regulated ( <0.05) in the co-cultured group in comparison with those of the control group. When compared with the si-NC group, the si- 1 group showed inhibited proliferation and migration ability of the co-cultured BMSCs; the percentage of G phase cells increased, while that of S phase decreased; the expression of 1, CyclinD1, 2 and 9 mRNA also decreased ( <0.05) in the si- 1 group.

Conclusion: The enhanced proliferation and migration ability of BMSCs in the glioma C6 microenvironment may be associated with the up-regulated expression of 1 .
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http://dx.doi.org/10.12182/20210560203DOI Listing
May 2021

METTL3/METTL14 Transactivation and mA-Dependent TGF-β1 Translation in Activated Kupffer Cells.

Cell Mol Gastroenterol Hepatol 2021 May 13. Epub 2021 May 13.

MOE Joint International Research Laboratory of Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, Jiangsu, P. R. China; Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, P. R. China. Electronic address:

Background And Aims: Transforming growth factor β1 (TGF-β1) secreted from activated Kupffer cells (KCs) promotes the progression of nonalcoholic steatohepatitis (NASH) to liver fibrosis. N6-methyladenosine (mA) RNA modification participates in various cell stress responses, yet it remains unknown whether it plays a role in TGF-β1 upregulation in activated KCs.

Methods: Western blot, dot blot, and liquid chromatography with tandem mass spectrometry were used to determine the expression of mA methyltransferase, METTL3, and METTL14, as well as global mA modification. RNA-sequencing and mA-seq were employed to screen differentially expressed genes and responsive mA peaks. Nuclear factor κB (NF-κB)-mediated METTL3/METTL14 transactivation were validated with chromatin immunoprecipitation polymerase chain reaction and dual-luciferase reporter system, and the role of mA in TGF-β1 upregulation was further verified in METTL3/METTL14-deficient KCs and myeloid lineage cell-specific METTL14 knockout mice.

Results: Serum lipopolysaccharide (LPS) concentration is increased in high-fat diet-induced NASH rats. TGF-β1 upregulation is closely associated with METTL3/METTL14 upregulation and global mA hypermethylation, in both NASH rat liver and LPS-activated KCs. LPS-responsive mA peaks are identified on the 5' untranslated region (UTR) of TGF-β1 messenger RNA (mRNA). NF-κB directly transactivates METTL3 and METTL14 genes. LPS-stimulated TGF-β1 expression is abolished in METTL3/METTL14-deficient KCs and myeloid lineage cell-specific METTL14 knockout mice. Mutation of mA sites on the 5'UTR of TGF-β1 mRNA blocks LPS-induced increase of luciferase reporter activity.

Conclusions: NF-κB acts as transcription factor to transactivate METTL3/METTL14 genes upon LPS challenge, leading to global RNA mA hypermethylation. Increased mA on the 5'UTR of TGF-β1 mRNA results in mA-dependent translation of TGF-β1 mRNA in a cap-independent manner. We identify a novel role of mA modification in TGF-β1 upregulation, which helps to shed light on the molecular mechanism of NASH progression.
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http://dx.doi.org/10.1016/j.jcmgh.2021.05.007DOI Listing
May 2021

Effect of advanced glycation end products on autophagic ability in osteoblasts.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):361-367

Department of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401145.

Objectives: Excessive production of AGEs in diabetic patients will affect the normal function of osteoblasts, and this process may be related to autophagy of osteoblasts. This study aims to explore the effect of advanced glycation end products (AGEs) on autophagic activity during osteogenic differentiation in rat bone marrow mesenchymal stem cells (BMSCs).

Methods: BMSCs were isolated and cultured in vitro, treated with different concentrations (0, 50, 100, 200, and 400 mg/L) of AGEs for different time (3, 6, 12, 24, 48, and 72 h). The proliferation activity was detected by CCK-8 method. The mRNA and protein expression levels of Beclin1 and LC3 in cells were detected by real-time PCR and Western blotting, respectively.The autophagic vacuoles were observed under the transmission electron microscope. The cells were treated with autophagy promoter rapamycin or autophagy inhibitor 3MA. After 7 days of osteogenic induction, we performed alkaline phosphatase (ALP) staining and real-time PCR to detect the mRNA expression levels of osteogenesis-related genes.

Results: In the low-concentration groups, the proliferation activity in BMSCs was increased (<0.01), the mRNA and protein expressions of autophagy-related genes LC3 and Beclin1 were increased (both <0.01). The number of autophagosome also was increased. In the high-concentration groups, the results were just the opposite. In the low-concentration groups, the ALP staining was deeper than that of the 0 mg/L AGEs group, and the mRNA expressions of the osteogenic related genes were increased (<0.01). But the results were reversed in the presence of autophagy inhibitor 3MA. In the high-concentration groups, the ALP staining was lighter than that of the 0 mg/L AGEs group, and the mRNA expressions of the osteogenic related genes were decreased (<0.01). After the addition of the autophagy promoter rapamycin, the results were reversed.

Conclusions: Low concentration of AGEs can enhance the proliferative activity of BMSCs and promote osteogenic differentiation by accelerating autophagy. High concentration of AGEs can suppress the proliferation of BMSCs and inhibit osteogenic differentiation by reducing autophagy.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.190401DOI Listing
April 2021

Embryonic exposure to hyper glucocorticoids suppresses brown fat development and thermogenesis via REDD1.

Sci Bull (Beijing) 2021 Mar 28;66(5):478-489. Epub 2020 Oct 28.

Nutrigenomics and Growth Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.

Maternal stress during pregnancy is prevailing worldwide, which exposes fetuses to intrauterine hyper glucocorticoids (GC), programming offspring to obesity and metabolic diseases. Despite the importance of brown adipose tissue (BAT) in maintaining long-term metabolic health, impacts of prenatal hyper GC on postnatal BAT thermogenesis and underlying regulations remain poorly defined. Pregnant mice were administrated with synthetic GC dexamethasone (DEX) at levels comparable to fetal GC exposure of stressed mothers. Prenatal GC exposure dose-dependently reduced BAT thermogenic activity, contributing to lower body temperature and higher mortality of neonates; such difference was abolished under thermoneutrality, underscoring BAT deficiency was the major contributor to adverse changes in postnatal thermogenesis due to excessive GC. Prenatal GC exposure highly activated expression and reduced transcription from the alternative promoter (-AP) in neonatal BAT. During brown adipocyte differentiation, ectopic expression reduced -AP expression and mitochondrial biogenesis; and the inhibitory effects of GC on mitochondrial biogenesis and -AP expression were blocked by ablation. reduced protein kinase A phosphorylation and suppressed cyclic adenosine monophosphate (cAMP) -responsive element-binding protein (CREB) binding to the cAMP regulatory element (CRE) in -AP promoter, leading to AP inactivation. In summary, excessive maternal GC exposure during pregnancy dysregulates --AP axis, which impairs fetal BAT development, hampering postnatal thermogenic adaptation and metabolic health of offspring.
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http://dx.doi.org/10.1016/j.scib.2020.10.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087250PMC
March 2021

Exposure to constant light impairs cognition with FTO inhibition and mA-dependent TrκB repression in mouse hippocampus.

Environ Pollut 2021 Aug 30;283:117037. Epub 2021 Mar 30.

MOE Joint International Research Laboratory of Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, 210095, PR China; Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address:

N6-methyladenosine (mA) mRNA methylation plays a role in various brain functions. Exposure to chronic constant light (CCL) has been reported to impair cognition, yet whether the underlying mechanism involves mA remains unknown. In this study, mice exposed to CCL for 3 weeks show impaired cognitive behavior, which was associated with increased mA level in hippocampus. Accordingly, the mA demethylase FTO was inhibited while the methyltransferases METTL3, METTL14 and WTAP, as well as the reader protein YTHDF2, were elevated in the hippocampus of CCL-exposed mice. CCL exposure significantly activated hippocampal expression of circadian regulator cryptochrome 1 and 2 (CRY1 and 2). Meanwhile, hippocampal neurogenesis was impaired with suppression of BDNF/TrκB/ERK pathway. To further delineate the signaling pathway and the role of mA, we altered the expression of CRY1/2 in hippocampus neuron cells. CRY1/2 overexpression inhibited FTO and increased mA levels, while CRY1/2 knockdown led to opposite results. Luciferase reporter analysis further confirmed CRY1/2-induced FTO suppression. Furthermore, FTO knockdown increased mA on 3'UTR of TrκB mRNA, and decreased TrκB mRNA stability and TrκB protein expression, in a YTHDF2-dependent manner. These results indicate that CCL-activated CRY1/2 causes transcriptional inhibition of FTO, which suppresses TrκB expression in hippocampus via mA-dependent post-transcriptional regulation and contributes to impaired cognitive behavior in mice exposed to constant light.
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http://dx.doi.org/10.1016/j.envpol.2021.117037DOI Listing
August 2021

High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression.

J Clin Endocrinol Metab 2021 Mar 24. Epub 2021 Mar 24.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Context: Regulatory T cells (Tregs) dysfunction plays an important role in the development and progression of Graves' disease (GD). Programmed cell death 1 (PD-1) prompts FoxP3 in Tregs expression and enhances the suppressive activity of Tregs. Whether abnormal expression of PD-1 contributes to the breakdown of Tregs and the role of thyroid hormone in the PD-1 expression of Tregs in GD remain substantially undefined.

Objective: To evaluate the role of PD-1 in Tregs function and triiodothyronine (T3) in PD-1 expression in patients with GD and mice treated with T3.

Methods: We recruited 30 patients with GD and 30 healthy donors. PD-1 expression in Tregs and Tregs function were determined. To evaluate the effects of thyroid hormone on PD-1 expression in Tregs, we used T3 for the treatment of human peripheral blood mononuclear cells (PBMCs). We then treated mice with T3 to confirm the effect of thyroid hormone on PD-1 expression in Tregs and Tregs function in vivo.

Results: PD-1 expression in Tregs and the suppressive function of Tregs significantly decreased in patients with GD. T3 reduced PD-1 expression in human Tregs in a concentration- and time-dependent manner in vitro. High levels of circulating T3 reduced PD-1 expression in Tregs, impaired Tregs function, and disrupted T-helper cell (Th1 and Th2) balance in mice treated with T3.

Conclusions: Tregs dysfunction in GD patients might be due to down-regulation of PD-1 expression in Tregs induced by high levels of serum T3.
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http://dx.doi.org/10.1210/clinem/dgab191DOI Listing
March 2021

Co-culture with Endothelial Progenitor Cells promotes the Osteogenesis of Bone Mesenchymal Stem Cells via the VEGF-YAP axis in high-glucose environments.

Int J Med Sci 2021 4;18(7):1628-1638. Epub 2021 Feb 4.

The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing, 401147, China.

Patients with type 2 diabetes mellitus (T2DM) have a high risk of fracture and experience poor bone healing. In recent years, bone mesenchymal stem cells (BMSCs) and endothelial progenitor cells (EPCs) have become the most commonly used cells in cell therapy and tissue engineering. In this study, we found that high glucose levels had a negative effect on the differentiation of BMSCs and EPCs. Considering that EPCs-BMSCs sheets can provide endothelial cells and osteoblastic cells, we transplanted cell sheets into T2DM rats with bilateral skull defects. The outcomes of the study revealed that EPCs-BMSCs sheets promoted ossification, which was verified by micro-CT and immunohistochemistry (IHC) analyses. Furthermore, we detected the VEGF content in the culture supernatant using an enzyme-linked immunosorbent assay (ELISA). The results showed that the BMSCs co-cultured with EPCs presented a higher level of VEGF than other cells. To assess the differentiation and migration of BMSCs exposed to VEGF, ALP staining, scratch assay and qRT-PCR analysis were performed. In addition, we used immunofluorescence and western blotting analysis to further explore the related mechanisms. The results showed that cells cultured with VEGF had a stronger actin cytoskeleton and a greater amount of nuclear and total YAP than cells cultured without VEGF. Taken together, our results indicate that co-culture with EPCs could promote the osteogenesis of BMSCs partially via VEGF. Furthermore, YAP and F-actin play important roles in this process.
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http://dx.doi.org/10.7150/ijms.52316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976568PMC
February 2021

Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing.

Nat Commun 2021 03 12;12(1):1657. Epub 2021 Mar 12.

School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel.

Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the root and refine the contributions of key players in this process. In addition, we determine the quantitative kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic insights into root development, the tools developed here will enable biologists to study kinetics and morphology of various critical processes at the single cell-level in whole organisms.
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http://dx.doi.org/10.1038/s41467-021-21802-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954861PMC
March 2021

Effects of mouth breathing on facial skeletal development in children: a systematic review and meta-analysis.

BMC Oral Health 2021 03 10;21(1):108. Epub 2021 Mar 10.

The Affiliated Stomatology Hospital of Chongqing Medical University, No.426 Songshi North Road, Yubei district, Chongqing, 401147, China.

Background: Mouth breathing is closely related to the facial skeletal development and malocclusion. The purpose of this systematic review and meta-analysis was to assess the effect of mouth breathing on facial skeletal development and malocclusion in children.

Methods: An electronic search in PubMed, the Cochrane Library, Medline, Web of Science, EMBASE and Sigle through February 23rd, 2020, was conducted. Inclusion criteria were children under 18 years of age with maxillofacial deformities due to mouth breathing. The risk of bias in nonrandomized studies of interventions (ROBINS-I) tool for controlled clinical trials. The Grading of Recommendation, Assessment, Development and Evaluation (GRADE) approach was used for the quality assessment. The included indicators were SNA, SNB, ANB, SN-OP, SN-PP, PP-MP, SNGoGn, MP-H, 1-NA, 1. NA, 1. NB, 1-NB, Overjet, Overbite, SPAS, PAS, and C3-H. Data concerning the mean difference in mesial molar movement and extent of canine retraction were extracted for statistical analysis. The mean differences and 95% confidence intervals were analyzed for continuous data. Review Manager 5.3, was used to synthesize various parameters associated with the impact of mouth breathing on facial skeletal development and malocclusion.

Results: Following full-text evaluations for eligibility, 10 studies were included in the final quantitative synthesis. In Sagittal direction, SNA (MD: - 1.63, P < 0.0001), SNB (MD: - 1.96, P < 0.0001) in mouth-breathing children was lower than that in nasal-breathing children. ANB (MD: 0.90, P < 0.0001), 1. NA (MD: 1.96, P = 0.009), 1-NA (MD: 0.66, P = 0.004), and 1-NB (MD: 1.03, P < 0.0001) showed higher values in children with mouth breathing. In vertical direction, SN-PP (MD: 0.68, P = 0.0050), SN-OP (MD: 3.05, P < 0.0001), PP-MP (MD: 4.92, P < 0.0001) and SNGoGn (MD: 4.10, P < 0.0001) were higher in mouth-breathing individuals. In airway, SPAS (MD: - 3.48, P = 0.0009), PAS (MD: - 2.11, P < 0.0001), and C3-H (MD: - 1.34, P < 0.0001) were lower in mouth breathing group.

Conclusions: The results showed that the mandible and maxilla rotated backward and downward, and the occlusal plane was steep. In addition, mouth breathing presented a tendency of labial inclination of the upper anterior teeth. Airway stenosis was common in mouth-breathing children. Trial registration [email protected], registration number CRD42019129198.
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http://dx.doi.org/10.1186/s12903-021-01458-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944632PMC
March 2021

Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation.

Front Chem 2021 18;9:649000. Epub 2021 Feb 18.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.

Cytochrome P450 enzyme CYP109B1 is a versatile biocatalyst exhibiting hydroxylation activities toward various substrates. However, the regio- and stereoselective steroid hydroxylation by CYP109B1 is far less explored. In this study, the oxidizing activity of CYP109B1 is reconstituted by coupling redox pairs from different sources, or by fusing it to the reductase domain of two self-sufficient P450 enzymes P450RhF and P450BM3 to generate the fused enzyme. The recombinant expressing necessary proteins are individually constructed and compared in steroid hydroxylation. The ferredoxin reductase (Fdr_0978) and ferredoxin (Fdx_1499) from is found to be the best redox pair for CYP109B1, which gives above 99% conversion with 73% 15β selectivity for testosterone. By contrast, the rest ones and the fused enzymes show much less or negligible activity. With the aid of redox pair of Fdr_0978/Fdx_1499, CYP109B1 is used for hydroxylating different steroids. The results show that CYP109B1 displayed good to excellent activity and selectivity toward four testosterone derivatives, giving all 15β-hydroxylated steroids as main products except for 9 (10)-dehydronandrolone, for which the selectivity is shifted to 16β. While for substrates bearing bulky substitutions at C17 position, the activity is essentially lost. Finally, the origin of activity and selectivity for CYP109B1 catalyzed steroid hydroxylation is revealed by computational analysis, thus providing theoretical basis for directed evolution to further improve its catalytic properties.
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http://dx.doi.org/10.3389/fchem.2021.649000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930613PMC
February 2021

Vitamin A and Its Multi-Effects on Pancreas: Recent Advances and Prospects.

Front Endocrinol (Lausanne) 2021 18;12:620941. Epub 2021 Feb 18.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Vitamin A (VA), which is stored in several forms in most tissues, is required to maintain metabolite homeostasis and other processes, including the visual cycle, energy balance, epithelial cell integrity, and infection resistance. In recent years, VA molecules, also known as retinoids, have been extensively explored and used in the treatment of skin disorders and immune-related tumors. To date, several observational and interventional studies have explored the relationship between VA status and the pathogenesis of diabetes. In particular, VA micronutrients have been shown to regulate pancreatic development, β-cell function, pancreatic innate immune responses, and pancreatic stellate cells phenotypes through multiple mechanisms. However, there are still many problems to be proven or resolved. In this review, we summarize and discuss recent and available evidence on VA biological metabolism in the pancreas. Analysis of the effects of VA on metabolism in the pancreas will contribute to our understanding of the supportive physiological roles of VA in pancreas protection.
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http://dx.doi.org/10.3389/fendo.2021.620941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930481PMC
February 2021

Changes of serum immunoglobulin level in healthy pregnant women and establishment of its reference interval.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Jan;46(1):53-59

Department of Laboratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011.

Objectives: Pregnant women in a special physiological period, the body's blood indicators will change to a certain extent. This study aims to explore the changes of serum immunoglobulin levels in healthy pregnant women and establish its reference interval (RI).

Methods: A total of 369 healthy pregnant women, who underwent pregnancy examination in the Department of Obstetrics, Second Xiangya Hospital of Central South University from August 2019 to October 2019, were enrolled for this study. They were divided into an early pregnancy group, a middle pregnancy group, and a late pregnancy group according to the pregnancy period, and 123 healthy non-pregnant women were selected as the controls. The levels of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) were determined by immune transmission turbidities. The level of immunoglobulin E (IgE) was determined by electrochemiluminescence. The differences in immunoglobulin levels between pregnant women and non-pregnant women and among different gestational periods were analyzed, and the RI of serum immunoglobulin level during pregnancy was established.

Results: Compared to the non-pregnant women, the levels of serum IgG, IgM, IgA, and IgE in pregnant women were significantly decreased (all <0.01), with 51.81% for IgG, 43.84% for IgM, 55.80% for IgA, and 49.80% for IgE. Except that the IgG level of late pregnancy group was significantly lower than that of early pregnancy group (<0.05), there were no significant differences in the IgG, IgM, IgA, and IgE levels among the other groups (all >0.05). The RIs of serum IgG in early pregnancy, middle pregnancy, and late pregnancy were 6.02-7.70 g/L, 5.18-6.85 g/L, and 4.58-5.72 g/L, respectively, while the RIs of serum IgM, IgA, and IgE were 0.71-0.93 g/L, 0.90-1.09 g/L, and 68.30-107.69 ng/mL, respectively in pregnant women.

Conclusions: The levels of immunoglobulin in pregnant women are decreased significantly. The establishment of RIs of IgG, IgM, IgA and IgE in healthy pregnant women could provide scientific basis for clinical decision-making.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200468DOI Listing
January 2021

Facile Fabrication of Superhydrophobic Cross-Linked Nanocellulose Aerogels for Oil-Water Separation.

Polymers (Basel) 2021 Feb 19;13(4). Epub 2021 Feb 19.

Institute of Chemical Industry of Forest Products, CAF, Key Lab. of Biomass Energy and Material, Key and Open Lab. of Forest Chemical Engineering, SFA, National Engineering Lab. for Biomass Chemical Utilization, Nanjing 210042, China.

A facile and environmental-friendly approach was developed for the preparation of the cross-linked nanocellulose aerogel through the freeze-drying process and subsequent esterification. The as-prepared aerogel had a three-dimensional cellular microstructure with ultra-low density of 6.05 mg·cm and high porosity (99.61%). After modifying by chemical vapor deposition (CVD) with hexadecyltrimethoxysilane (HTMS), the nanocellulose aerogel displayed stable super-hydrophobicity and super-oleophilicity with water contact angle of 151°, and had excellent adsorption performance for various oil and organic solvents with the adsorption capacity of 77~226 g/g. Even after 30 cycles, the adsorption capacity of the nanocellulose aerogel for chloroform was as high as 170 g/g, indicating its outstanding reusability. Therefore, the superhydrophobic cross-linked nanocellulose aerogel is a promising oil adsorbent for wastewater treatment.
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http://dx.doi.org/10.3390/polym13040625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921982PMC
February 2021

Retraction Note to: Circ-TFCP2L1 Promotes the Proliferation and Migration of Triple Negative Breast Cancer through Sponging miR-7 by Inhibiting PAK1.

J Mammary Gland Biol Neoplasia 2021 Mar 2. Epub 2021 Mar 2.

Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.

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http://dx.doi.org/10.1007/s10911-021-09481-8DOI Listing
March 2021

Rapid Changes in Serum Testosterone in Men With Newly Diagnosed Type 2 Diabetes With Intensive Insulin and Metformin.

Diabetes Care 2021 Apr 3;44(4):1059-1061. Epub 2021 Feb 3.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Jiangsu, China

Objective: To investigate the effect of metformin on testosterone levels in men with type 2 diabetes mellitus (T2DM).

Research Design And Methods: Seventy men with newly diagnosed drug-naive T2DM and HbA >9.0% (75 mmol/mol) were treated with intensive insulin pump therapy for 5 days to achieve glucose normalization. They were randomized to control (continued on intensive insulin only) and metformin (plus metformin) groups (1:1) for 1 month. Testosterone was measured at baseline, randomization, and after 1-month treatment.

Results: Total, free, and bioavailable testosterone increased significantly within 5 days (all < 0.001). After 1 month, compared with the control group, the metformin group had lower total (12.7 vs. 15.3 nmol/L), free (0.20 vs. 0.24 nmol/L), and bioavailable (4.56 vs. 5.31 nmol/L) testosterone (all < 0.05).

Conclusions: In men with T2DM, 1-month oral metformin may decrease serum testosterone levels independent of blood glucose control. The effects of long-term metformin on testosterone in men need further study.
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http://dx.doi.org/10.2337/dc20-1558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985426PMC
April 2021

Practice of precision surgery in primary liver cancer.

Hepatobiliary Pancreat Dis Int 2021 Apr 22;20(2):108-109. Epub 2021 Jan 22.

Department of General Surgery, Huzhou Central Hospital, Huzhou 313003, China. Electronic address:

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http://dx.doi.org/10.1016/j.hbpd.2021.01.004DOI Listing
April 2021

Effects of integrated biocontrol on bacterial wilt and rhizosphere bacterial community of tobacco.

Sci Rep 2021 Jan 29;11(1):2653. Epub 2021 Jan 29.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China.

Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
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http://dx.doi.org/10.1038/s41598-021-82060-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846572PMC
January 2021

Toll-like Receptor 9 Agonists as Adjuvants for Nanoparticle-Based Nicotine Vaccine.

Mol Pharm 2021 03 26;18(3):1293-1304. Epub 2021 Jan 26.

Department of Biological Systems Engineering, Virginia Tech, Blacksburg, Virginia 24061, United States.

Nicotine vaccine was considered a promising therapy against smoking addiction. The level of immune response that a nicotine vaccine can induce is pivotal to its efficacy. In this study, Toll-like receptor 9 agonists, namely, CpG ODN 1555 and CpG ODN 1826, were incorporated into a nanoparticle-based nicotine vaccine (NanoNicVac) to enhance its immunogenicity. The results showed that NanoNicVac containing either CpG ODN 1555 or CpG ODN 1826 could be rapidly internalized by dendritic cells. In mice trials, it was found that NanoNicVac with CpG ODN 1555 and CpG ODN 1826 induced 3.3- and 3.2-fold higher anti-nicotine antibody titer than that by the native NanoNicVac after two injections, respectively. Instead of enhancing the immunogenicity of the vaccine, however, mixtures of the two CpG ODNs were observed to exert an immune-suppressing effect on NanoNicVac. Finally, the histopathological examination on major organs of the mice immunized with the NanoNicVacs proved that NanoNicVac with either CpG ODN 1555 or CpG ODN 1826 as adjuvants did not cause detectable toxicity to the mice.
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http://dx.doi.org/10.1021/acs.molpharmaceut.0c01153DOI Listing
March 2021

Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China.

Biomed Environ Sci 2020 Dec;33(12):893-905

Department of Traditional Chinese Medicine, The First Hospital of Suihua City, Suihua 152053, Heilongjiang, China.

Objective: Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.

Methods: A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients' medical records. Multivariable logistic regression models were used to estimate the odds ratio ( ) and 95% confidence interval (95% ) of the associations between comorbidities (cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.

Results: Overall, 158 (13.6%) patients were diagnosed with severe illness and 32 (2.7%) had unfavorable outcomes. Hypertension (2.87, 1.30-6.32), type 2 diabetes (T2DM) (3.57, 2.32-5.49), cardiovascular disease (CVD) (3.78, 1.81-7.89), fatty liver disease (7.53, 1.96-28.96), hyperlipidemia (2.15, 1.26-3.67), other lung diseases (6.00, 3.01-11.96), and electrolyte imbalance (10.40, 3.00-26.10) were independently linked to increased odds of being severely ill. T2DM (6.07, 2.89-12.75), CVD (8.47, 6.03-11.89), and electrolyte imbalance (19.44, 11.47-32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission (5.46, 3.25-9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes (6.58, 1.46-29.64) within two weeks.

Conclusion: Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes.
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http://dx.doi.org/10.3967/bes2020.123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817475PMC
December 2020

Addition of TLR9 agonist immunotherapy to radiation improves systemic antitumor activity.

Transl Oncol 2021 Feb 16;14(2):100983. Epub 2020 Dec 16.

Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Radiotherapy (RT) has been used to control tumors by physically damaging DNA and inducing apoptosis; it also promotes antitumor immune responses via neoantigens release and augmenting immune-oncology agents to elicit systemic response. Tumor regression after RT can recruit inflammatory cells, such as tumor-associated macrophages and CD11b myeloid cell populations, a major subset of which may actually be immunosuppressive. However, these inflammatory cells also express Toll-like receptors (TLRs) that can be stimulated to reverse suppressive characteristics and promote systemic antitumor outcomes. Here, we investigated the effects of adding CMP-001, a CpG-A oligodeoxynucleotide TLR9 agonist delivered in a virus-like particle (VLP), to RT in two murine models (344SQ metastatic lung adenocarcinoma and CT26 colon carcinoma). High-dose RT (12Gy x 3 fractions) significantly increased the percentages of plasmacytoid dendritic cells within the tumor islets 3- and 5-days post-RT; adding CMP-001 after RT also enhanced adaptive immunity by increasing the proportion of CD4 and CD8 T cells. RT plus CMP-001-mediated activation of the immune system led to significant inhibition of tumor growth at both primary and abscopal tumor sites, thereby suggesting a new combinatorial treatment strategy for systemic disease.
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http://dx.doi.org/10.1016/j.tranon.2020.100983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750418PMC
February 2021

Rice transcription factor MADS32 regulates floral patterning through interactions with multiple floral homeotic genes.

J Exp Bot 2021 03;72(7):2434-2449

Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Floral patterning is regulated by intricate networks of floral identity genes. The peculiar MADS32 subfamily genes, absent in eudicots but prevalent in monocots, control floral organ identity. However, how the MADS32 family genes interact with other floral homeotic genes during flower development is mostly unknown. We show here that the rice homeotic transcription factor OsMADS32 regulates floral patterning by interacting synergistically with E class protein OsMADS6 in a dosage-dependent manner. Furthermore, our results indicate important roles for OsMADS32 in defining stamen, pistil, and ovule development through physical and genetic interactions with OsMADS1, OsMADS58, and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3, and OsMADS58, respectively. Our findings suggest that OsMADS32 is an important factor for floral meristem identity maintenance and that it integrates the action of other MADS-box homeotic proteins to sustain floral organ specification and development in rice. Given that OsMADS32 is an orphan gene and absent in eudicots, our data substantially expand our understanding of flower development in plants.
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http://dx.doi.org/10.1093/jxb/eraa588DOI Listing
March 2021

Needle-free jet injection of insulin glargine improves glycemic control in patients with type 2 diabetes mellitus: a study based on the flash glucose monitoring system.

Expert Opin Drug Deliv 2021 Feb 19:1-7. Epub 2021 Feb 19.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Jiangsu, China.

: To investigate the effects of insulin glargine injection given with a QS-P jet injector on the glucose profile using a professional mode flash glucose monitoring (FGM) system in patients with type 2 diabetes mellitus (T2DM).: In this randomized, controlled, cross-sectional study, 66 patients with T2DM who received insulin glargine (12-18 IU/day) injection were enrolled. The patients were randomly divided into group A (jet injector before insulin pen) and group B (insulin pen before jet injector). Each subject injected insulin daily before breakfast. We analyzed the changes in the glucose profile using a professional mode FGM system.: Treatment with a jet injector led to significantly lower 24-h mean glucose, maximum blood glucose, area under the curve (AUC) > 10.0 mmol/L, time above range and increased AUC < 3.9 mmol/L and time below range than those when using an insulin pen. There was no difference in glycemic variability between the two groups. We observed that patients using a jet injector had significantly lower mean glucose between 12:00 to 22:00.: Needle-free jet injection of insulin glargine was more effective than use of an insulin pen for good glycemic control in patients with T2DM.: www.clinicaltrials.gov identifier is NCT04093284.
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http://dx.doi.org/10.1080/17425247.2021.1863945DOI Listing
February 2021

Effect of Dapagliflozin on Glycemic Variability in Patients with Type 2 Diabetes under Insulin Glargine Combined with Other Oral Hypoglycemic Drugs.

J Diabetes Res 2020 24;2020:6666403. Epub 2020 Nov 24.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, China.

Aim: To evaluate the effect of an inhibitor of sodium-glucose cotransporter 2 (SGLT-2 inhibitor, dapagliflozin) on glycemic variability in type 2 diabetes mellitus (T2D) under insulin glargine combined with oral hypoglycemic drugs, using a continuous glucose monitoring system (CGMS).

Methods: This prospective, self-controlled, single-center clinical trial recruited 36 patients with T2D under combined insulin glargine and oral hypoglycemic drugs. General clinical data were collected. Fasting blood glucose (FBG), postprandial blood glucose (PBG), glycosylated hemoglobin (HbA1c), and C-peptide levels were assessed before and four weeks of dapagliflozin (10 mg per day) treatment. Blood glucose was monitored for 72 hours before and after treatment using CGMS.

Results: After treatment with dapagliflozin, FBG decreased from 6.74 ± 1.78 to 5.95 ± 1.13 mmol/L ( < 0.05); PBG decreased from 13.04 ± 2.99 to 10.92 ± 3.26 mmol/L ( < 0.05); HbA1c decreased from 7.37 ± 0.96% to 6.94 ± 0.80%. The proportion of patients with HbA1c < 7% increased from 27.8% to 58.3%, and the proportion of patients with HbA1c < 7% and without level 2 hypoglycemia increased from 27.8% to 55.6% ( < 0.05). CGMS data showed reduction of the 24 h MBG, MAGE, time-above-range (TAR, >10 mmol/L), high blood glucose index (HBGI), glucose management indicator (GMI), and incremental area under the curve of the glucose level more than 10 mmol/L (AUC > 10) and an increase of time-in-range (TIR, 3.9-10 mmol/L) with treatment. Homeostasis model assessment for pancreatic beta-cell function (HOMA-beta) increased significantly with treatment ( < 0.05), and fewer insulin doses were required after the treatment, without increasing in hypoglycemia and urinary tract infection. Further, a stratified analysis showed that patients with higher pretreatment HbA1c and waist-to-hip ratio (WHR) had greater improvement in glycemic control.

Conclusion: Dapagliflozin may reduce blood glucose levels, ameliorate glycemic variability, and improve pancreatic beta-cell function in patients with T2D under insulin glargine combined with other oral hypoglycemic drugs, especially in those with poor glucose control and abdominal obesity.
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http://dx.doi.org/10.1155/2020/6666403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707966PMC
November 2020

Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids.

J Anim Sci Biotechnol 2020 Nov 2;11(1):106. Epub 2020 Nov 2.

College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, PR China.

Background: Time-restricted feeding (TRF) is a dieting strategy based on nutrients availability and diurnal rhythm, shown to improve lipid metabolism efficiency. We have demonstrated previously that retinoic acid-related (RAR) orphan receptor (ROR) γ is the primary transcription factor controlling cholesterol (CHO) biosynthesis program of animals. However, the functional role of RORγ in liver physiology of pigs in response to TRF has not been determined, largely due to the lack of functional models and molecular tools. In the present study, we established porcine liver organoids and subjected them to restricted nutrients supply for 10-h during the light portion of the day.

Results: Our results showed that TRF regimen did not alter hepatocyte physiology, including unchanged cell viability, caspase 3/7 enzyme activity and the gene signature of cell proliferation in porcine liver organoids, compared to the control group (P > 0.05). Furthermore, we found that TRF downregulated the hepatic CHO biosynthesis program at both mRNA and protein levels, along with the reduced cellular CHO content in porcine liver organoids (P < 0.05). Using unbiased bioinformatic analysis of a previous ChIP-seq data and ChIP-qPCR validation, we revealed RORγ as the predominant transcription factor that responded to TRF, amongst the 12 targeted nuclear receptors (NRs) (P < 0.05). This was likely through RORγ direct binding to the MVK gene (encoding mevalonate kinase). Finally, we showed that RORγ agonists and overexpression enhanced the enrichment of co-factor p300, histone marks H3K27ac and H3K4me1/2, as well as RNA polymerase II (Pol-II) at the locus of MVK, in TRF-porcine liver organoids, compared to TRF-vector control (P < 0.05).

Conclusions: Our findings demonstrate that TRF triggers the RORγ-mediated chromatin remodeling at the locus of CHO biosynthesis genes in porcine liver organoids and further improves lipid metabolism.
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http://dx.doi.org/10.1186/s40104-020-00511-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604961PMC
November 2020

A parameter-free framework for calibration enhancement of near-infrared spectroscopy based on correlation constraint.

Anal Chim Acta 2021 Jan 8;1142:169-178. Epub 2020 Nov 8.

Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health/School of Food Science, Guizhou Medical University, Guiyang, 550025, China.

A new parameter-free framework for calibration enhancement (PFCE) was proposed for dealing with the near-infrared (NIR) spectral inconsistency and maintaining the prediction ability of the calibration model under different conditions. The calibration issues encountered in the maintenance with or without using standards, and even the enhancement between instruments have been thoroughly addressed. The general calibration maintenance/enhancement cases were formulated into non-supervised PFCE (NS-PFCE), semi-supervised PFCE (SS-PFCE), and full-supervised PFCE (FS-PFCE). The NS-PFCE made use of both the provided master and slave spectra of standard samples to construct a maintained calibration slave model by implementing a correlation constraint on the regression coefficients. The SS-PFCE and FS-PFCE methods integrated the slave spectra and reference information of standard samples at the same time into the slave spectral calibration, and thus a maintenance or enhancement model could be achieved for the slave spectra, in particular measured on different instruments. The use of dataset1 comprised of 655 pharmaceutical tablets measured on two NIR spectrometers and datset2 containing 117 plant leaf samples in two mesh sizes has demonstrated that the PFCE framework had a significant effect on enhancing the predictions of the slave spectra in the models. The root mean square errors of prediction (RMSEPs) of either active pharmaceutical ingredient (API) amount in tablets or reducing sugar content in plant leaf samples from the slave spectra approached to or were lower than those values predicted from the master spectra in the master models established with the partial least-squares (PLS) regression method. The advantage of PFCE was parameter-free and efficient. First, the method could be flexibly employed in scientific or applicative environment with no regard to the parameter specification. Second, the performance of NS-PFCE was comparable to the classical calibration maintenance methods, yet the SS-PFCE and FS-PFCE could enhance the prediction ability to a level widely considered as the upper boundary of classical calibration maintenance methods reached.The source code of the method is available at https://github.com/JinZhangLab/PFCE.
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http://dx.doi.org/10.1016/j.aca.2020.11.006DOI Listing
January 2021

Corrigendum to "Circ-EIF4G3 promotes the development of gastric cancer by sponging miR-335" [Pathol. - Res. Pract. 215 (2019) 152507].

Pathol Res Pract 2021 Jan 30;217:153163. Epub 2020 Nov 30.

Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China. Electronic address:

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http://dx.doi.org/10.1016/j.prp.2020.153163DOI Listing
January 2021