Publications by authors named "Yun Deng"

269 Publications

Design, synthesis and biological evaluation of tyrosinase-targeting PROTACs.

Eur J Med Chem 2021 Oct 2;226:113850. Epub 2021 Oct 2.

Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. Electronic address:

The human tyrosinase is the most prominent therapeutic target for pigmentary skin disorders. However, the overwhelming majority efforts have been devoted to search mushroom tyrosinase inhibitors, which show poor inhibitory activity on human tyrosinase and certain side effects that cause skin damage in practical application. Herein, a series of degraders that directly targeted human tyrosinase was firstly designed and synthesized based on newly developed PROTAC technology. The best PROTAC TD9 induced human tyrosinase degradation obviously in dose and time-dependent manner, and its mechanism of inducing tyrosinase degradation has also been clearly demonstrated. Besides, encouraging results that low-toxicity PROTAC TD9 was applied to reduce zebrafish melanin synthesis have been obtained, highlighting the potential to treatment of tyrosinase-related disorders. Moreover, this work has innovatively expanded the application scope of PROTAC technology and laid a solid foundation for further development of novel drugs treating pigmentary skin disorders.
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http://dx.doi.org/10.1016/j.ejmech.2021.113850DOI Listing
October 2021

Risk assessment and source apportionment of trace elements in multiple compartments in the lower reach of the Jinsha River, China.

Sci Rep 2021 Oct 8;11(1):20041. Epub 2021 Oct 8.

China Three Gorges Projects Development Co., Ltd,, Chengdu, 610000, China.

Studies on trace element (TE) pollution in abiotic matrices have typically focused on water, sediment, and soil, either separately or in pairs. The importance of multi-media connectivity has been ignored. This study analyzed the concentrations of 6 TEs in three connected environmental compartments of a 28-km section of the lower reach of the Jinsha River. The ecological risk posed by TEs was higher in soil than in sediment. The contribution of exposure pathways to human health risk were ranked as ingestion > dermal contact > inhalation. An improved regional environmental risk index (RERI) method was then developed to evaluate the comprehensive risk on both ecology and human health caused by TEs. The average RERI value was generally higher in the wet season (0.42) than in the dry (0.41) and dry-to-wet transition seasons (0.08) because of the combined effects of the high TE concentrations in riparian soil and the long exposure time. Source apportionment indicated that industrial activities, weathering of parent rock, and agricultural activities were possible sources of TEs in this region. The methods and results of this study could inform local environmental management and provide references for similar cases wherein multiple compartments of river systems should be considered.
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http://dx.doi.org/10.1038/s41598-021-99626-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501140PMC
October 2021

Upregulated IL-10 induced by E2F2-miR-17-5p circuitry in extrafollicular effector B cells contributes to autoantibody production in systemic lupus erythematosus.

Arthritis Rheumatol 2021 Sep 27. Epub 2021 Sep 27.

Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.

Objective: Elevated interleukin 10 (IL10) in SLE patients has B-cell promoting effects, contributing to autoantibody production and tissue damage. We aimed to characterize upregulated IL10 B-cell subsets and dysregulated IL10 expression in SLE B cells for new therapeutic options.

Methods: Proportions of Th10 and IL10 B-cell subsets in PBMCs were assessed using flow cytometry. The IL10-3'UTR dual-luciferase vector was constructed and co-transfected with siRNA, miRNA mimics or inhibitors into RAJI cell line. Transcript levels were quantified using Taqman assays.

Results: Culture conditions that induced IL10 Breg in healthy controls (HC) resulted in expansion of IL10 DN2 (IgD CD27 CD21 CD11c ) B-cells in SLE PBMCs. Proportions of IL10 DN2, but not those of IL10 DN2, correlated with disease activity, levels of antibodies to dsDNA, and associated with high levels or seropositivity of anti-Smith and anti-cardiolipin IgG in SLE patients from two cohorts of mainly African Americans and Asians, respectively. Proportions of Th10 (CD45RA CXCR5 CXCR3 PD1 CD4 ) cells correlated with IL10 DN2 frequencies, ANA titers and proteinuria levels in SLE patients. Screening predicted IL10 3'UTR-targeting miRNAs in SLE B cells identified miR-17-5p and miR-20a-5p with their levels inversely correlated with IL10 and transcription factor E2F2. In RAJI cells, knockdown of E2F2 expression resulted in increased levels of miR-17-5p and miR-20a-5p and decreased IL10 mRNA and protein levels, and overexpression and inhibition of miR-17-5p down-regulated and up-regulated IL10 mRNA levels respectively; suggesting regulation of IL10 expression by E2F2-miR-17-5p loop.

Conclusion: IL10 promotes extrafollicular autoimmune responses in active SLE patients, which might be dampened by targeting the E2F2-miR-17-5p circuitry.
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http://dx.doi.org/10.1002/art.41987DOI Listing
September 2021

Human SLE variant -R90H promotes kidney damage and murine lupus through enhanced Tfh2 responses induced by defective efferocytosis of macrophages.

Ann Rheum Dis 2021 Sep 23. Epub 2021 Sep 23.

Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA

Objectives: We previously identified a hypomorphic variant, p.Arg90His (p.R90H) of neutrophil cytosolic factor 1 ( a regulatory subunit of phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 complex), as an putative causal variant for systemic lupus erythematosus (SLE), and established a knock-in (KI) H90 variant in the C57BL/6 background to study how this variant promotes lupus development.

Methods: Wild type (WT) and KI littermates were assessed for immune profiles and lupus-like features. Disease activity and renal damage of patients with SLE were assessed by systemic lupus erythematosus disease activity index (SLEDAI) and renal items of systemic lupus international collaborating clinics (SLICC), respectively.

Results: Compared with WT littermates, 5-week-old homozygous KI mice had reduced oxidative burst, splenomegaly, elevated type I interferon (IFN-I) scores, increased ratios of splenic follicular T helper 2 (Tfh2) to either T follicular regulatory (Tfr) or Tfh1 cells, increased ANA follicular, germinal centre and plasma cells without spontaneous kidney disease up to 1 year of age. Pristane treatment exacerbated the immune dysregulation and induced IFN-I-dependent kidney disease in 36-week-old H90 KI female mice. Decreased efferocytosis of macrophages derived from KI mice and patients with homozygous H90 SLE promoted elevated ratios of Tfh2/Tfr and Tfh2/Tfh1 as well as dysregulated humoral responses due to reduced voltage-gated proton channel 1 (Hv1)-dependent acidification of phagosome pH to neutralise the decreased electrogenic effect of the H90 variant, resulting in impaired maturation and phagosome proteolysis, and increased autoantibody production and kidney damage in mice and patients with SLE of multiple ancestries.

Conclusions: A lupus causal variant, NCF1-H90, reduces macrophage efferocytosis, enhances Tfh2 responses and promotes autoantibody production and kidney damage in both mice and patients with SLE.
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http://dx.doi.org/10.1136/annrheumdis-2021-220793DOI Listing
September 2021

The Indication of Poor Prognosis by High Expression of ENO1 in Squamous Cell Carcinoma of the Lung.

J Oncol 2021 30;2021:9910962. Epub 2021 Aug 30.

Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, Guangxi, China.

The purpose of this study is to investigate the significance of alpha-enolase (ENO1) expression in squamous cell carcinoma of the lung (LUSC), its prognostic value, and prospective molecular mechanism. Using multiplatforms data, including in-house immunohistochemistry, in-house real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), in-house microarray, and public high-throughput data, the expression significance and prognostic role of ENO1 in LUSC tissues were analyzed comprehensively. With the combination of all eligible cases, compared with 941 non-LUSC lung tissues, ENO1 was significantly overexpressed in 1163 cases of LUSC (standardized mean difference (SMD) = 1.23, 95% confidence interval (CI) = 0.76-1.70,  < 0.001). ENO1 also displayed a great ability to differentiate LUSC tissues from non-LUSC lung tissues (AUC = 0.8705) with the comprehensive sensitivity being 0.88 [0.83-0.92], and comprehensive specificity being 0.89 [0.84-0.94]). Moreover, in 1860 cases of LUSC with survival information, patients with higher expression of ENO1 had poorer prognosis (hazard ratio (HR) = 1.20, 95% CI = 1.01-1.43,  = 0.043). ENO1 and its related genes mainly participated in the pathways of cell division and proliferation. In conclusion, the upregulation of ENO1 could affect the carcinogenesis and unfavorable outcome of LUSC.
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http://dx.doi.org/10.1155/2021/9910962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423576PMC
August 2021

Temporal and Spatial Signaling Mediating the Balance of the Plankton Microbiome.

Ann Rev Mar Sci 2021 Aug 26. Epub 2021 Aug 26.

Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany; email:

The annual patterns of plankton succession in the ocean determine ecological and biogeochemical cycles. The temporally fluctuating interplay between photosynthetic eukaryotes and the associated microbiota balances the composition of aquatic planktonic ecosystems. In addition to nutrients and abiotic factors, chemical signaling determines the outcome of interactions between phytoplankton and their associated microbiomes. Chemical mediators control essential processes, such as the development of key morphological, physiological, behavioral, and life-history traits during algal growth. These molecules thus impact species succession and community composition across time and space in processes that are highlighted in this review. We focus on spatial, seasonal, and physiological dynamics that occur during the early association of algae with bacteria, the exponential growth of a bloom, and also during its decline and recycling. We also discuss how patterns from field data and global surveys might be linked to the actions of metabolic markers in natural phytoplankton assemblages. Expected final online publication date for the , Volume 14 is January 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
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http://dx.doi.org/10.1146/annurev-marine-042021-012353DOI Listing
August 2021

Three Previously Undescribed Chlorophenyl Glycosides from the Bulbs of Lilium regale.

Chem Biodivers 2021 Oct 2;18(10):e2100403. Epub 2021 Sep 2.

State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, P. R. China.

Three previously undescribed chlorophenyl glycosides, (2,4,6-trichloro-3-hydroxy-5-methoxyphenyl)methyl β-D-glucopyranoside (1), (2,4-dichloro-3,5-dimethoxyphenyl)methyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside (2) and 4-chloro-3-methoxy-5-methylphenyl 6-O-(6-deoxy-β-L-mannopyranosyl)-β-D-glucopyranoside (3) were obtained from Lilium regale. The absolute configurations of these new finds were elucidated by comprehensive analyses of spectroscopic data combined with acid hydrolysis derivatization. (2,4-dichloro-3,5-dimethoxyphenyl)methyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside (2) can inhibit the proliferation of lung carcinoma A549 cells with an IC value of 29 μΜ.
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http://dx.doi.org/10.1002/cbdv.202100403DOI Listing
October 2021

A rapid quality grade discrimination method for Gastrodia elata powderusing ATR-FTIR and chemometrics.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 16;264:120189. Epub 2021 Jul 16.

School of Big Data, Yunnan Agricultural University, 650201 Kunming, China. Electronic address:

Gastrodia elata is an obligate fungal symbiont used in traditional Chinese medicine. There are currently 4 grades of the plant based on the "Commodity Specification Standard of 76 Kinds of Medicinal Materials". The traditional discrimination methods for determining the medicinal grade of G. elata powders are complex and time-consuming which are not suitable for rapid analysis. We developed a rapid analysis method for this plant using attenuated total reflection and Fourier-transform infrared spectroscopy (ATR-FTIR) together with machine learning algorithms. The original spectroscopic data was first pre-treated using the multiplicative scatter correction (MSC) method and 4 principal components were extracted using extremely randomized trees (Extra-trees) and principal component analysis (PCA) algorithms, and different kinds of classification models were established. We found that multilayer perceptron classifier (MLPC) modeling was superior to support vector machine (SVM) and resulted in validation and prediction accuracies of 99.17% and 100%, respectively and a modeling time of 2.48 s. The methods established from the current study can rapidly and effectively distinguish the 4 different types of G. elata powders and thus provides a platform for rapid quality inspection.
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http://dx.doi.org/10.1016/j.saa.2021.120189DOI Listing
July 2021

On-DNA Derivatization of Quinoxalin-2-ones by Visible-Light-Triggered Alkylation with Carboxylic Acids.

Bioconjug Chem 2021 08 4;32(8):1576-1580. Epub 2021 Aug 4.

HitGen Inc., Building 6, No. 8 Huigu First East Road, Tianfu International Bio-Town, Shuangliu District, Chengdu 610000, Sichuan, P.R. China.

An efficient visible-light-induced alkylation of DNA-tagged quinoxaline-2-ones was described. The methodology demonstrated moderate-to-excellent conversions under mild conditions. The reaction was found to be tolerant with both -protected α-amino acids and aliphatic carboxylic acids and could be applied to the synthesis of focused DNA-encoded quinoxalin-2-one libraries.
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http://dx.doi.org/10.1021/acs.bioconjchem.1c00346DOI Listing
August 2021

Specific recognition, intracellular assay and detoxification of fluorescent curcumin derivative for copper ions.

J Hazard Mater 2021 10 25;420:126490. Epub 2021 Jun 25.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address:

Recognition and excretion of metal ions play an important role in the diagnosis and treatment of various diseases and poisoning. Although copper (Cu) is a cofactor of many key enzymes in the human body, its accumulation caused by genetic ATP7B mutation or environmental pollution can lead to hepatotoxicity, renal failure, Wilson's disease, inflammation, and even Parkinson's disease (PD) and Alzheimer's disease (AD). Therefore, in this work, a difluoroboron curcumin derivative (DF-Cur) was used for the specific recognition of copper ions (Cu). DF-Cur could be further used to as a rapid diagnostic agent for the copper detection in cells and zebrafish at the nanomolar level. DF-Cur could significantly reduce the toxic damage caused by high Cu dose. Inductively coupled plasma-mass spectrometry (ICP-MS) analysis indicated that DF-Cur could promote the excretion of copper ions in the urine and bile and reduce the accumulation of copper ions in vivo. In addition, DF-Cur could selectively detect cholesterol in the blood and adipose tissue in vivo by fluorescent staining. These results demonstrated that this molecule might represent a new and promising diagnostic and therapeutic agent to combat diseases related to copper ions accumulation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126490DOI Listing
October 2021

[Thermal desorption sampling-gas chromatography-tandem mass spectrometry with a carbonyl-iron powder composite silica monolithic column for enrichment and determination of pyrethroid pesticide residues in tea samples].

Se Pu 2020 Aug;38(8):961-967

Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

Long-term exposure to pyrethroid insecticides is detrimental to the nervous system, reproductive system, and immune system in humans. Therefore, enrichment detection of pyrethroid pesticides is imperative. In this study, a novel carbonyl-iron powder composite silica monolithic column was first prepared for the enrichment of pyrethroid pesticide residues in tea samples. Then, the target analytes were thermally desorbed and online-injected into a gas chromatography-tandem mass spectrometry (GC-MS/MS) system. In the present method, hydroxy-terminated polydimethylsiloxane (PDMS) was covalently bonded to the surface of the SiO network and subsequently bonded with the carbonyl-iron powder. After the target analytes were adsorbed and concentrated in the PDMS spots, high-frequency induction heating was used for GC-MS/MS sampling. Under the optimal conditions, the detection limits of the pyrethroid pesticide residues were 3.8 to 7.5 μg/kg, and the relative standard deviation was 3.2% to 6.8% (=6). The extraction recovery ranged from 97.7% to 110.5%, and the correlation coefficient was ≥ 0.9960. In addition, the enrichment factor could reach 1000 times. PDMS materials show excellent adsorption properties for non-polar solutes. In our experiment, carbonyl iron powder-bonded monolithic columns were prepared on the basis of stir bar sorptive extraction (SBSE). Carbonyl iron powder magnetic particles were evenly implanted into the inorganic-organic hybrid cassia material for realizing rapid and uniform desorption upon electromagnetic induction heating. Under the premise of perfectly integrating the technical advantages of SBSE and solid-phase microextraction (SPME), the electromagnetic induction characteristics of carbonyl iron powder can be exploited for thermal desorption and directly combined with GC-MS to facilitate online analysis and solvent-free elution. Compared with the conventional SPME method, the proposed method has the advantages of high enrichment factor, large adsorption capacity of the column, reusability, high degree of automation, and good universality. This method has high significance for sample preparation and for the extraction of pesticide residues in complex matrices.
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http://dx.doi.org/10.3724/SP.J.1123.2019.12019DOI Listing
August 2020

Loss of Leads to Skeletal Deformities and Downregulation of Bone Developmental Pathway in Zebrafish.

Int J Mol Sci 2021 Jun 22;22(13). Epub 2021 Jun 22.

Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha 430100, China.

Wingless-type MMTV integration site family, member 16 (), is a wnt ligand that participates in the regulation of vertebrate skeletal development. Studies have shown that can regulate bone metabolism, but its molecular mechanism remains largely undefined. We obtained the zebrafish model using the CRISPR-Cas9-mediated gene knockout screen with 11 bp deletion in , which led to the premature termination of amino acid translation and significantly reduced expression, thus obtaining the zebrafish model. The expression of in bone-related parts was detected via in situ hybridization. The head, spine, and tail exhibited significant deformities, and the bone mineral density and trabecular bone decreased in using light microscopy and micro-CT analysis. RNA sequencing was performed to explore the differentially expressed genes (DEGs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that the down-regulated DEGs are mainly concentrated in mTOR, FoxO, and VEGF pathways. Protein-protein interaction (PPI) network analysis was performed with the detected DEGs. Eight down-regulated DEGs including akt1, bnip4, ptena, vegfaa, twsg1b, prkab1a, prkab1b, and pla2g4f.2 were validated by qRT-PCR and the results were consistent with the RNA-seq data. Overall, our work provides key insights into the influence of gene on skeletal development.
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http://dx.doi.org/10.3390/ijms22136673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268848PMC
June 2021

The distribution of waiting distances in ancestral recombination graphs.

Theor Popul Biol 2021 10 26;141:34-43. Epub 2021 Jun 26.

Department of Statistics, University of California, Berkeley, CA 94720, United States of America; Department of Integrative biology, University of California, Berkeley, CA 94720, United States of America. Electronic address:

The ancestral recombination graph (ARG) contains the full genealogical information of the sample, and many population genetic inference problems can be solved using inferred or sampled ARGs. In particular, the waiting distance between tree changes along the genome can be used to make inference about the distribution and evolution of recombination rates. To this end, we here derive an analytic expression for the distribution of waiting distances between tree changes under the sequentially Markovian coalescent model and obtain an accurate approximation to the distribution of waiting distances for topology changes. We use these results to show that some of the recently proposed methods for inferring sequences of trees along the genome provide strongly biased distributions of waiting distances. In addition, we provide a correction to an undercounting problem facing all available ARG inference methods, thereby facilitating the use of ARG inference methods to estimate temporal changes in the recombination rate.
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http://dx.doi.org/10.1016/j.tpb.2021.06.003DOI Listing
October 2021

The degradation mechanisms of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) by different chemical methods: A critical review.

Chemosphere 2021 Nov 13;283:131168. Epub 2021 Jun 13.

School of Environment, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China. Electronic address:

Per- and polyfluoroalkyl substances (PFASs) are a class of artificial compounds comprised of a perfluoroalkyl main chain and a terminal functional group. With them being applied in a wide range of applications, PFASs have drawn increasing regulatory attention and research interests on their reductions and treatments due to their harmful effects on environment and human beings. Among numerous studies, chemical treatments (e.g., photochemical, electrochemical, and thermal technologies) have been proved to be important methods to degradation PFASs. However, the pathways and mechanisms for the degradation of PFASs through these chemical methods still have not been well documented. This article therefore provides a comprehensive review on the degradation mechanisms of two important PFASs (perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS)) with photochemical, electrochemical and thermal methods. Different decomposition mechanisms of PFOA and PFOS are reviewed and discussed. Overall, the degradation pathways of PFASs are associated closely with their head groups and chain lengths, and H/F exchange and chain shortening were found to be predominant degradation mechanisms. The clear study on the degradation mechanisms of PFOA and PFOS should be very useful for the complete degradation or mineralization of PFASs in the future.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131168DOI Listing
November 2021

3,3',5-Triiodothyroacetic acid (TRIAC) induces embryonic ζ-globin expression via thyroid hormone receptor α.

J Hematol Oncol 2021 06 26;14(1):99. Epub 2021 Jun 26.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The human ζ-globin gene (HBZ) is transcribed in primitive erythroid cells only during the embryonic stages of development. Reactivation of this embryonic globin synthesis would likely alleviate symptoms both in α-thalassemia and sickle-cell disease. However, the molecular mechanisms controlling ζ-globin expression have remained largely undefined. Moreover, the pharmacologic agent capable of inducing ζ-globin production is currently unavailable. Here, we show that TRIAC, a bioactive thyroid hormone metabolite, significantly induced ζ-globin gene expression during zebrafish embryogenesis. The induction of ζ-globin expression by TRIAC was also observed in human K562 erythroleukemia cell line and primary erythroid cells. Thyroid hormone receptor α (THRA) deficiency abolished the ζ-globin-inducing effect of TRIAC. Furthermore, THRA could directly bind to the distal enhancer regulatory element to regulate ζ-globin expression. Our study provides the first evidence that TRIAC acts as a potent inducer of ζ-globin expression, which might serve as a new potential therapeutic option for patients with severe α-thalassemia or sickle-cell disease.
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http://dx.doi.org/10.1186/s13045-021-01108-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235803PMC
June 2021

Analysis of the characteristic compounds of Citri Sarcodactylis Fructus from different geographical origins.

Phytochem Anal 2021 Jun 10. Epub 2021 Jun 10.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Introduction: Citri Sarcodactylis Fructus (CSF) is widely used as a food ingredient and a traditional Chinese medicine. In China, CSF is cultivated in many places, including Sichuan, Guangdong, Zhejiang, and Fujian provinces. The types and chemical contents of CSF from different origins may vary greatly due to the difference in climate and environmental conditions. Therefore, comparing the chemical composition of CSF from various places is vital.

Objective: To rapidly select potential characteristic compounds for differentiating CSF from different origins.

Material And Methods: Thirty-one batches of CSF samples from different regions were analysed using ultra-performance liquid chromatography with hybrid quadrupole-orbitrap high-resolution mass spectrometry. Thereafter, chemometric methods, including principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA), were employed to find differential metabolites among the CSF samples from various origins.

Results: PCA revealed 77.9% of the total variance and divided all CSF samples into three categories corresponding to their origins. OPLS-DA displayed better discrimination of CSF from different sources, with R X, R Y, and Q of 0.801, 0.985, and 0.849, respectively. Finally, 203 differential metabolites were obtained from CSF from different origins using the variable importance in projection of the OPLS-DA model, 30 of which were identified, and five coumarin compounds were selected as marker compounds discriminating CSF from different origins.

Conclusion: This work provides a practical strategy for classifying CSF from different origins and offers a research foundation for the quality control of CSF.
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http://dx.doi.org/10.1002/pca.3069DOI Listing
June 2021

Roles of Lytic Viral Replication and Co-Infections in the Oncogenesis and Immune Control of the Epstein-Barr Virus.

Cancers (Basel) 2021 May 10;13(9). Epub 2021 May 10.

Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, 8057 Zürich, Switzerland.

Epstein-Barr virus (EBV) is the prototypic human tumor virus whose continuous lifelong immune control is required to prevent lymphomagenesis in the more than 90% of the human adult population that are healthy carriers of the virus. Here, we review recent evidence that this immune control has not only to target latent oncogenes, but also lytic replication of EBV. Furthermore, genetic variations identify the molecular machinery of cytotoxic lymphocytes as essential for this immune control and recent studies in mice with reconstituted human immune system components (humanized mice) have begun to provide insights into the mechanistic role of these molecules during EBV infection. Finally, EBV often does not act in isolation to cause disease. Some of EBV infection-modulating co-infections, including human immunodeficiency virus (HIV) and Kaposi sarcoma-associated herpesvirus (KSHV), have been modeled in humanized mice. These preclinical in vivo models for EBV infection, lymphomagenesis, and cell-mediated immune control do not only promise a better understanding of the biology of this human tumor virus, but also the possibility to explore vaccine candidates against it.
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http://dx.doi.org/10.3390/cancers13092275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126045PMC
May 2021

Antimicrobial, antioxidant and physical properties of chitosan film containing Akebia trifoliata (Thunb.) Koidz. peel extract/montmorillonite and its application.

Food Chem 2021 Nov 15;361:130111. Epub 2021 May 15.

Department of Food Science and Technology, 100 Wiegand Hall, Oregon State University, Corvallis, OR, USA.

A novel active packaging film was prepared in this study that incorporated Akebia trifoliata (Thunb.) Koidz. peel extracts (APE) and montmorillonite (MMT) into chitosan (CH) films. Compared with the pure CH film, the CH/APE film showed significantly higher tensile strength, elongation at break, UV light resistance, and antibacterial activity; the CH/MMT film displayed significant increases in contact angle, antioxidant activity, oxygen permeability, and thermal stability. SEM and AFM analyses showed that the additions were well-distributed into the CH matrix, but MMT induced a more compact and rougher structure. The CH-based film formula was optimized using the single-factor test and Box-Behnken design and was 0.15% MMT, 0.15% APE, and 1.50% CH. Besides, the optimized coating was applied in the postharvest preservation of A. trifoliata fruits, which yielded a significant effect on the delaying crack and mature of the fruits during 35 days of storage at 5 °C.
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http://dx.doi.org/10.1016/j.foodchem.2021.130111DOI Listing
November 2021

Flipped Quick-Response Code Enables Reliable Blood Grouping.

ACS Nano 2021 04 19;15(4):7649-7658. Epub 2021 Apr 19.

Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044, People's Republic of China.

Accurate and rapid blood typing plays a vital role in a variety of biomedical and forensic scenarios, but recognizing weak agglutination remains challenging. Herein, we demonstrated a flipping identification with a prompt error-discrimination (FLIPPED) platform for automatic blood group readouts. Bromocresol green dye was exploited as a characteristic chromatography indicator for the differentiation of plasma from whole blood by presenting a teal color against a brown color. After integrating these color changes into a quick-response (QR) code, prompt typing of ABO and Rhesus groups was automatically achieved and data could be uploaded wirelessly within 30 s using a commercially available smartphone to facilitate blood cross-matching. We further designed a color correction model and algorithm to remove potential errors from scanning angles and ambient light intensities, by which weak agglutination could be accurately recognized. With comparable accuracy and repeatability to classical column assay in grouping 450 blood samples, the proposed approach further demonstrates to be a versatile sample-to-result platform for clinical diagnostics, food safety, and environmental monitoring.
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http://dx.doi.org/10.1021/acsnano.1c01215DOI Listing
April 2021

CD27 is required for protective lytic EBV antigen-specific CD8+ T-cell expansion.

Blood 2021 Jun;137(23):3225-3236

Viral Immunobiology, Institute of Experimental Immunology, and.

Primary immunodeficiencies in the costimulatory molecule CD27 and its ligand, CD70, predispose for pathologies of uncontrolled Epstein-Barr virus (EBV) infection in nearly all affected patients. We demonstrate that both depletion of CD27+ cells and antibody blocking of CD27 interaction with CD70 cause uncontrolled EBV infection in mice with reconstituted human immune system components. While overall CD8+ T-cell expansion and composition are unaltered after antibody blocking of CD27, only some EBV-specific CD8+ T-cell responses, exemplified by early lytic EBV antigen BMLF1-specific CD8+ T cells, are inhibited in their proliferation and killing of EBV-transformed B cells. This suggests that CD27 is not required for all CD8+ T-cell expansions and cytotoxicity but is required for a subset of CD8+ T-cell responses that protect us from EBV pathology.
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http://dx.doi.org/10.1182/blood.2020009482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351885PMC
June 2021

An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L.

Food Res Int 2021 02 24;140:109984. Epub 2020 Dec 24.

Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau. Electronic address:

High hydrostatic pressure (HHP) technique is used as a novel abiotic stress factor for efficiently enhancing the biosynthesis of selected bioactive phytochemicals in germinated wholegrain, but the information about HHP stress-induced metabolic changes remains rather limited. Thus, the current work employed an untargeted gas chromatography-mass spectrometry-based metabolomic approach combining with multivariate models to analyze the effect of mild HHP stress (30 MPa/5 min) on the overall metabolome shifts of wholegrain brown rice (WBR) during germination. Simultaneously, major phenolics in germinated WBR (GBR) were detected by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to explore the potential relationship between HHP stress-induced rice metabolome alternations and the biotransformation of bioactive components. The results demonstrated that the influence of HHP stress on GBR metabolite profiles was defined by germination durations, as revealed by the differentiation of the stressed grains from the naturally germinated grains at different germination points according to principal component analysis. This was further confirmed by the results of orthogonal projections to latent structures discriminant analysis, in which the discriminating metabolites between naturally germinated and HHP-stressed grains varied across the germination process. The metabolite signatures differentiating natural and HHP-stressed germination included glycerol-3-phosphate, monosaccharides, gamma-aminobutyric acid, 2,3-butanediol, glyceryl-glycoside, amino acids and myo-inositol. Besides, HHP stress led to the increase in ribose, arabinitol, salicylic acid, azelaic acid and gamma-aminobutyric acid, as well as the reduced phenolic acids. These results demonstrated that HHP stress before germination matched with appropriate process parameters could be used as a promising technology to tailor metabolic features of germinated products, thus exerting targeted nutrition and health implications.
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http://dx.doi.org/10.1016/j.foodres.2020.109984DOI Listing
February 2021

Streptochlorin analogues as potential antifungal agents: Design, synthesis, antifungal activity and molecular docking study.

Bioorg Med Chem 2021 04 14;35:116073. Epub 2021 Feb 14.

Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Streptochlorin is a small molecule of indole alkaloid isolated from marine Streptomyces sp., it is a promising lead compound due to its potent bioactivity in preventing many phytopathogens in our previous study, but further structural modifications are required to improve its antifungal activity. Our work in this paper focused on the replacement of oxazole ring in streptochlorin with the imidazole ring, to discover novel analogues. Based on this design strategy, three series of streptochlorin analogues were efficiently synthesized through sequential Vilsmeier-Haack reaction, Van Leusen imidazole synthesis and halogenation reaction. Some of the analogues displayed excellent activity in the primary assays, and this is highlighted by compounds 4g and 4i, the growth inhibition against Alternaria Leaf Spot and Rhizoctorzia solani under 50 μg/mL are 97.5% and 90.3%, respectively, even more active than those of streptochlorin, pimprinine and Osthole. Molecular docking models indicated that streptochlorin binds with Thermus thermophiles Leucyl-tRNA Synthetase in a similar mode to AN2690, offering a perspective on the mode of action study for antifungal activities of streptochlorin derivatives. Further study is still ongoing with the aim of discovering synthetic analogues, with improved antifungal activity and clear mode of action.
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http://dx.doi.org/10.1016/j.bmc.2021.116073DOI Listing
April 2021

Immunomodulatory activity of puerarin in RAW264.7 macrophages and cyclophosphamide-induced immunosuppression mice.

Immunopharmacol Immunotoxicol 2021 Apr 14;43(2):223-229. Epub 2021 Feb 14.

Department of Pharmacy, Medical School, Anhui University of Science and Technology, Huainan, P. R. China.

Context: Puerarin, a natural isoflavone extracted from , is famous for treating various cardiovascular and cerebrovascular diseases. However, little is known about its direct immunomodulatory activity.

Objective: This study was designed to investigate the and immunomodulatory effects of by using the murine monocyte-macrophage cell line RAW264.7 and immunosuppressed cyclophosphamide-induced mice.

Methods: MTT and neutral red phagocytosis assays were conducted to evaluate the immunomodulatory activities of puerarin on cell viability and phagocytosis by measuring the proliferation, phagocytic, nitric oxide (NO) ability, and TNF-α production ability of stimulated and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Immunosuppressed cyclophosphamide-induced mice were used to evaluate the immunomodulatory activities of puerarin by measuring IL-4 and IFN-γ, the serum half hemolysis value, spleen and thymus index, and proliferation assay for splenic lymphocytes.

Results And Discussion: Results showed that puerarin improves immunomodulatory activity by increasing cell proliferation, cell phagocytosis, and NO secretion in RAW264.7 macrophages and reduces the abnormal immunologic activity by decreasing cell phagocytosis and NO secretion in LPS-stimulated RAW264.7 macrophages. In addition, puerarin enhanced the immunologic activity of cyclophosphamide-induced immunosuppression mice by increasing the secretion of NO, IFN-γ, and IL-4, the serum half hemolysis value (HC), the spleen and thymus index, and proliferation for splenic lymphocytes.

Conclusion: Puerarin exhibited an upregulated immunomodulatory effect on RAW264.7 macrophages and immunosuppression mice. In addition, puerarin had a downregulated immunomodulatory effect on RAW264.7 macrophages. The results suggest that puerarin could be a promising immunomodulator to assist in the treatment of tumors.
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http://dx.doi.org/10.1080/08923973.2021.1885043DOI Listing
April 2021

PD-1 Blockade Aggravates Epstein-Barr Virus Post-Transplant Lymphoproliferative Disorder in Humanized Mice Resulting in Central Nervous System Involvement and CD4 T Cell Dysregulations.

Front Oncol 2020 12;10:614876. Epub 2021 Jan 12.

Laboratory of Regenerative Immune Therapies Applied, REBIRTH - Research Center for Translational Regenerative Medicine, Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany.

Post-transplant lymphoproliferative disorder (PTLD) is one of the most common malignancies after solid organ or allogeneic stem cell transplantation. Most PTLD cases are B cell neoplasias carrying Epstein-Barr virus (EBV). A therapeutic approach is reduction of immunosuppression to allow T cells to develop and combat EBV. If this is not effective, approaches include immunotherapies such as monoclonal antibodies targeting CD20 and adoptive T cells. Immune checkpoint inhibition (ICI) to treat EBV PTLD was not established clinically due to the risks of organ rejection and graft--host disease. Previously, blockade of the programmed death receptor (PD)-1 by a monoclonal antibody (mAb) during infection of mononuclear cells with the EBV/M81 strain showed lower xenografted lymphoma development in mice. Subsequently, fully humanized mice infected with the EBV/B95-8 strain and treated with a PD-1 blocking mAb showed aggravation of PTLD and lymphoma development. Here, we evaluated in fully humanized mice after EBV/B95-8 or EBV/M81 infections the effects of a clinically used PD-1 blocker. Fifteen to 17 weeks after human CD34 stem cell transplantation, Nod.Rag.Gamma mice were infected with two types of EBV laboratory strains expressing firefly luciferase. Dynamic optical imaging analyses showed systemic EBV infections and this triggered vigorous human CD8 T cell expansion. Pembrolizumab administered from 2 to 5 weeks post-infections significantly aggravated EBV systemic spread and, for the M81 model, significantly increased the mortality of mice. ICI promoted Ki67CD30CD20EBERPD-L1 PTLD with central nervous system (CNS) involvement, mirroring EBV CNS PTLD in humans. PD-1 blockade was associated with lower frequencies of circulating T cells in blood and with a profound collapse of CD4 T cells in lymphatic tissues. Mice treated with pembrolizumab showed an escalation of exhausted T cells expressing TIM-3, and LAG-3 in tissues, higher levels of several human cytokines in plasma and high densities of FoxP3 regulatory CD4 and CD8 T cells in the tumor microenvironment. We conclude that PD-1 blockade during acute EBV infections driving strong CD8 T cell priming decompensates T cell development towards immunosuppression. Given the variety of preclinical models available, our models conferred a cautionary note indicating that PD-1 blockade aggravated the progression of EBV PTLD.
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http://dx.doi.org/10.3389/fonc.2020.614876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837057PMC
January 2021

Triptolide delivery: Nanotechnology-based carrier systems to enhance efficacy and limit toxicity.

Pharmacol Res 2021 03 20;165:105377. Epub 2021 Jan 20.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Institute of Integrated Bioinformedicine & Translational Science, Hong Kong Baptist University Shenzhen Research Institute and Continuing Education, Shenzhen, 518000, China; Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, 999077, China. Electronic address:

Triptolide (TP) possesses a wide range of biological and pharmacological activities involved in the treatment of various diseases. However, widespread usages of TP raise the urgent issues of the severe toxicity, which hugely limits its further clinical application. The novel functional nanostructured delivery system, which is of great significance in enhancing the efficacy, reducing side effects and improving bioavailability, could improve the enrichment, penetration and controlled release of drugs in the lesion location. Over the past decades, considerable efforts have been dedicated to designing and developing a variety of TP delivery systems with the intention of alleviating the adverse toxicity effects and enhancing the bioavailability. In this review, we briefly summarized and discussed the recent functionalized nano-TP delivery systems for the momentous purpose of guiding further development of novel TP delivery systems and providing perspectives for future clinical applications.
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http://dx.doi.org/10.1016/j.phrs.2020.105377DOI Listing
March 2021

Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway.

Inflammation 2021 Jun 5;44(3):890-898. Epub 2021 Jan 5.

Center for Reproductive Medicine, The 904th Hospital of Joint Logistic Support Force, PLA, 101 Xingyuan North Road, Wuxi, 214043, Jiangsu, China.

Tadalafil (TAD) is primarily a treatment drug for erectile dysfunction. Studies have shown that TAD has a therapeutic effect on prostatitis, but the specific mechanism has not been reported. LPS induced RWPE-1 cells to form a model of chronic nonbacterial prostatitis (CNP). Cell activity was measured by MTT assay. Apoptosis was detected by TUNEL assay. Western blot was used to detect the expression of apoptosis-related proteins Bcl-2, Bax, Caspase-3, and cleaved caspase3. ELISA was used to detect the expression of inflammatory cytokines TNF-α, IL-6, and IL-8. GSH, catalase (CAT), and malondialdehyde (MDA) kits were used to detect the expression of oxidative stress-related indicators GSH, CAT, and MDA. Western blot was used to detect the expression of proteins related to Akt/Nrf2 signaling pathway. After different concentrations of TAD were given, the survival rate of LPS-induced RWPE-1 cells decreased, apoptosis increased, and inflammation and oxidative stress decreased. This process is accompanied by the activation of the Akt/Nrf2 signaling pathway. The addition of AKT inhibitor (HY-10249A) reversed the inhibitory effect of TAD on LPS-induced inflammatory response and oxidative stress of RWPE-1 cell. TAD alleviated LPS-induced inflammation and oxidative stress of RWPE-1 cell by regulating the Akt/Nrf2 signaling pathway.
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http://dx.doi.org/10.1007/s10753-020-01384-wDOI Listing
June 2021

Study of the thermal regime of a reservoir on the Qinghai-Tibetan Plateau, China.

PLoS One 2020 21;15(12):e0243198. Epub 2020 Dec 21.

State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, China.

The Qinghai-Tibetan Plateau region has unique meteorological characteristics, with low air temperature, low air pressure, low humidity, little precipitation, and strong diurnal variation. A two-dimensional hydrodynamic CE-QUAL-W2 model was configured for the Pangduo Reservoir to better understand the thermal structure and diurnal variation inside the reservoir under the local climate and hydrological conditions on the Qinghai-Tibetan Plateau. Observation data were used to verify the model, and the results showed that the average error of the 6 profile measured monthly from August to December 2016 was 0.1°C, and the root-mean-square error (RMSE) was 0.173°C. The water temperature from August 2016 to September 2017 was simulated by inputting measured data as model inputs. The results revealed that the reservoir of the Qinghai-Tibetan Plateau was a typical dimictic reservoir and the water mixed vertically at the end of March and the end of October. During the heating period, thermal stratification occurred, with strong diurnal variation in the epilimnion. The mean variance of the diurnal water temperature was 0.10 within a 5 m water depth but 0.04 in the whole water column. The mixing mode of inflow changed from undercurrent, horizontal-invaded flow and surface layer flow in one day. In winter, the diurnal variation was weak due to the thermal protection of the ice cover, while the mean variance of diurnal water temperature was 0.00 within both 5 m and the whole water column. Compared to reservoirs in areas with low altitude but the same latitude, significant differences occurred between the temperature structure of the low-altitude reservoir and the Pangduo Reservoir (P<0.01). The Pangduo Reservoir presented a shorter stratification period and weaker stratification stability, and the annual average SI value was 26.4 kg/m2, which was only 7.5% that of the low-altitude reservoir. The seasonal changes in the net heat flux received by the surface layers determined the seasonal cycle of stratification and mixing in reservoirs. This study provided a scientific understanding of the thermal changes in stratified reservoirs under the special geographical and meteorological conditions on the Qinghai-Tibetan Plateau. Moreover, this model can serve as a reference for adaptive management of similar dimictic reservoirs in cold and high-altitude areas.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243198PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751983PMC
January 2021

Understanding synergistic mechanisms of ferrous iron activated sulfite oxidation and organic polymer flocculation for enhancing wastewater sludge dewaterability.

Water Res 2021 Feb 21;189:116652. Epub 2020 Nov 21.

School of Environmental Studies, China University of Geosciences, Wuhan 430074, Hubei, China. Electronic address:

The bound water in waste activated sludge (WAS) is trapped in extracellular polymeric substances (EPS) in the form of gel-like structure, leading to a great challenge in the sludge deep dewatering. Traditional flocculation conditioning is unable to destroy EPS and ineffective to remove the bound water in WAS. In this study, we employed integration of Fe(II)-sulfite oxidation and polyacrylamide flocculation (F/S-PAM) treatment for removing the bound water and improving sludge dewaterability under aerobic conditions. Meanwhile, the floc microstructure and EPS properties were examined to understand the mechanisms of F/S-PAM conditioning. F/S produced SO radicals which could decompose the EPS in sludge, releasing bound water into free water. In addition, the formed Fe(III) from F/S led to re-coagulation of decomposed EPS, and C=O groups of tryptophan played the leading role in Fe-EPS association binding, causing transformation of the secondary structure of proteins (especially β-sheets and α-helices). Then, the introduction of PAM caused re-flocculation of disintegrated sludge flocs, enhancing the sludge filterability. This work provides a novel and cost-effective method for efficient removal of bound water in sludge, and subsequence improvement in sludge dewaterability.
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http://dx.doi.org/10.1016/j.watres.2020.116652DOI Listing
February 2021

Litterfall biomass and nutrient cycling in karst and nearby non-karst forests in tropical China: A 10-year comparison.

Sci Total Environ 2021 Mar 12;758:143619. Epub 2020 Nov 12.

CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Yunnan 666303, China; Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Yunnan 666303, China. Electronic address:

Litterfall helps maintaining primary production and nutrient cycling in forest ecosystems. However, few studies have investigated long-term characteristics of litterfall in tropical karst and non-karst forests co-occurring in the same region. A 10-year comparative study was conducted to estimate the biomass, litter accumulation, turnover rate, nutrient return and nutrient use efficiency associated with litterfall in a karst forest (KF) and a nearby non-karst forest (NKF) in northern tropical China. Significant spatial-temporal variation was observed in monthly and annual litterfall biomass in the two forests. Annual mean litterfall biomass in KF (9.75 Mg ha year) was obviously lower than that in NKF (10.49 Mg ha year). The litterfall biomass in NKF was significantly correlated with maximum air temperature, wind speed and total solar radiation, whereas that in KF was significantly correlated with relative humidity, wind speed and low temperature. Average stand litter in KF (2.92 Mg ha year) was significantly higher than that in NKF (2.38 Mg ha year). Stand litter mostly occurred during the cool and dry season, which coincided with litterfall input and exhibited bimodal pattern. Turnover rate was 1.3 time higher in NKF than in KF, suggesting that litter decomposed slowly in karst habitats. Distinct temporal dynamic and significant differences were observed in chemical composition of litterfall between KF and NKF. Total amounts of C, P, K and total nutrients returned to the topsoil in KF were significantly lower than those in NKF. The KF exhibited relatively high P and K use efficiency because of their low availability in karst soils. Compared with the non-karst habitat, the tropical karst habitats are more likely to develop a plant community with certain nutrient concentrations of litterfall and with a nutrient cycling mechanism that is well-adapted to harsh and heterogeneous condition.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143619DOI Listing
March 2021

Phenolic glycosides from and their anti-inflammatory effects.

Nat Prod Res 2020 Nov 18:1-8. Epub 2020 Nov 18.

The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, State Key Laboratory, Breeding Base of Systematic Research Development and Utilization of Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Two new phenolic glycosides 7,8-threo-4,7,9,9'-tetrahydroxy-3-methoxy-8--4'-neolignan-3'--(3''-α-L-arabinofuranosyl)--D-glucopyranoside. (), 4-(4'-hydroxyphenyl)-2-butanone-4''--(6--D-xylosyl)-β--glucopyranoside (), along with two known related analogues 7,8-threo-4,7,9,9'-tetrahydroxy-3-methoxy-8--4'-neolignan-3'--β--glucopyranoside (), 4-(4'-hydroxyphenyl)-2-butanone-4'--β--glucopyranoside () were obtained from the roots of ffi. Combined with acid hydrolysis derivatization, the absolute configurations of these new compounds were elucidated by comprehensive analyses of spectroscopic data including nuclear magnetic resonance (NMR), electrospray ionization high resolution mass (HRESIMS) as well as circular dichroism (CD). Compounds - exhibited anti-inflammatory properties in vitro by attenuating the production of inflammatory mediators, such as nitric oxide (NO) as well as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).
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http://dx.doi.org/10.1080/14786419.2020.1849202DOI Listing
November 2020
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