Publications by authors named "Yumei Sun"

41 Publications

Quercetin as an antiviral agent inhibits the Pseudorabies virus in vitro and in vivo.

Virus Res 2021 Sep 4:198556. Epub 2021 Sep 4.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430000, China. Electronic address:

Pseudorabies virus (PRV) accounts for a critical swine disease incurring economic losses worldwide. Several PRV vaccines are commercially available but these vaccines are effective against only certain prevalent PRV strains in China. To prevent PRV-induced latent infection and decrease the pathogenicity, novel anti-PRV drugs are required to prevent PRV infection. Natural products show exceptional structural diversity representing an important source for developing novel therapeutic agents. Quercetin is a flavonoid with anti-oxidant, anti-cancer, anti-bacterial and anti-viral activities. This study involved quercetin for studying the anti-PRV function in vitro and in vivo. Quercetin was found to significantly decrease the PRV virulent strain HNX at a half-maximal inhibitory concentration (IC) of 2.618 μM and selectivity index 229. This anti-PRV activity of quercetin was found to be dose-dependent. Furthermore, quercetin also inhibited a wide the infections by a spectrum of PRV strains like HNX, Ea, Bartha and Fa strain. These virucidal effects of quercetin suggest the interaction between these molecules and viral particles, and quercetin is responsible for inhibiting the adsorption of PRV infections. The silico assays suggesting that quercetin might interact with the gD-protein on the surface of the PRV important for viral infection. Additional, the quercetin plantar injection protected the mice from the lethal challenge, decreasing the PRV-infected mice's brain viral loads and mortality. These results provides a anti-PRV strategy and contribute to drug discovery and development.
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http://dx.doi.org/10.1016/j.virusres.2021.198556DOI Listing
September 2021

Incidence of retinopathy of prematurity treatment in extremely preterm infants in China.

Paediatr Perinat Epidemiol 2021 Sep 1. Epub 2021 Sep 1.

Children's Hospital of Fudan University, National Children's Medical Centre, Shanghai, China.

Background: Retinopathy of prematurity (ROP) continues to be a major cause of visual impairment and blindness in premature infants and children.

Objectives: To investigate the incidence of severe ROP receiving treatment in extremely preterm (EP) infants in China over time. The risk factors for ROP treatment were also assessed.

Methods: This was a multicentre retrospective study and a subanalysis of baseline data from the "Outcomes of EP infants in China 2010-2019" study. This study was conducted in 68 tertiary neonatal care centres from 31 provinces of China. Infants with a gestational age of 23 -27  weeks and admitted to a neonatal unit within the first 72 h of life between 2010 and 2019 were enrolled. Incidence of ROP was analysed in infants who survived to 32 weeks postmenstrual age and screened for ROP. Multivariable modified Poisson regression models were used to identify risk factors for ROP treatment.

Results: Among 7295 eligible infants, 4701 (64.5%) survived to 32 weeks postmenstrual age and met ROP screening criteria. Of the 3756 infants who screened and with ROP data, 2320 (61.8%) developed ROP of any stage. The overall incidence of ROP treatment was 12.6%, decreasing from 45.5% at 23 weeks to 8.3% at 27 weeks. During the 10-year period, the incidence of ROP treatment did not change, although the incidence of any ROP increased over time. Independent risk factors associated with ROP treatment included lower gestational age, small for gestational age, multiple birth, severe intraventricular haemorrhage, patent ductus arteriosus and supplemental oxygen duration.

Conclusions: The incidence of EP infants receiving ROP treatment showed no change during this 10-year period in China. Prevention of prematurity and foetal growth restriction, judicious use of oxygen and reducing comorbidities are promising factors that may reduce the incidence of ROP needing treatment in these high-risk infants.
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http://dx.doi.org/10.1111/ppe.12810DOI Listing
September 2021

Prevalence and correlates of lifestyle behavior, anxiety and depression in Chinese college freshman: A cross-sectional survey.

Int J Nurs Sci 2021 Jul 5;8(3):347-353. Epub 2021 Jun 5.

Division of Humanity & Social Sciences, School of Nursing, Peking University, Beijing, China.

Objectives: First-year college students had exposure to unhealthy lifestyle behaviors that correlate with a high prevalence of anxiety and depression. Regarding to the modifiable lifestyle behaviors factors, this study investigated the prevalence and correlation of multiple lifestyle behaviors, anxiety and depression in a sample of Chinese first-year college students.

Methods: Cross-sectional data were extracted from app of health lifestyle behaviors survey from September to October 2019. Anxiety, depression, eating regular meals, consumption of snacks in-between meals, consumption of fruit, dessert and sugar-sweetened beverages, smoking and secondhand smoke exposure, consuming alcohol, physical activity, sedentary time were assessed by self-report. Socio-demographic including age, gender, education, family income, religion, and health condition were captured. Logistic regression was used to explore the association of multiple lifestyle behaviors, anxiety and depression.

Results: Totally 1,017 participants were included in the study. The prevalence of anxiety and depression (from mild to severe) were 40.3% and 45.3%, respectively. In multivariable analyses, religion (believe in Buddhism,  = 2.438, 95%CI: 1.097-5.421; believe in Christian,  = 5.886, 95%CI: 1.604-21.597), gender (Female,  = 1.405, 95%CI: 1.001-1.971), secondhand smoke exposure ( = 1.089, 95%CI: 1.001-1.184), and eating regular meals ( = 0.513, 95%CI: 0.346-0.759) were associated with anxiety. Family income ( = 0.732, 95%CI: 0.596-0.898), eating regular meals ( = 0.641, 95%CI: 0.415-0.990), frequency of breakfast ( = 0.813, 95%CI: 0.690-0.959), with a chronic disease ( = 1.902, 95%CI: 1.335-2.712), and consumption of nocturnal snack ( = 1.337, 95%CI: 1.108-1.612) were associated with depression.

Conclusions: These results highlighted the need for early lifestyle behavior intervention, especially modifying diet patterns considering the background of religion, health condition, and social-economic status in first-year college students to improve their mental health.
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http://dx.doi.org/10.1016/j.ijnss.2021.05.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283720PMC
July 2021

Leptin regulates OPG and RANKL expression in Gingival Fibroblasts and Tissues of Chronic Periodontitis Patients.

Int J Med Sci 2021 22;18(11):2431-2437. Epub 2021 Apr 22.

Central of Stomatology, Xiangya Hospital of Central South University, Changsha, China.

Chronic periodontitis is a bone-destructive disease affecting periodontal support structures. Although leptin has a protective effect against periodontitis, the underlying mechanism remains unclear. Therefore, this study aimed to investigate the possible role of leptin by examining its relationship with OPG and RANKL in human gingival tissues obtained from patients with chronic periodontitis. Twenty-two patients with chronic periodontitis were enrolled (10 with moderate periodontitis and 12 with severe periodontitis) in the experimental group, and 12 healthy individuals were enrolled in the control group. Gingival tissue samples were collected, and the protein levels and localization of leptin, OPG, and RANKL were studied using immunohistochemistry (IHC). The staining intensities of leptin, OPG, and RANKL were correlated with the periodontal clinical index. Moreover, real-time quantitative PCR (RT-qPCR) was used to determine and mRNA levels in gingival fibroblasts stimulated with gradient concentrations of leptin protein . Leptin, OPG, and RANKL were located in the cytoplasm of gingival epithelial cells and the connective tissue. Leptin was widely and significantly expressed in the control group, whereas it was lightly stained in the severe group. RANKL was lightly stained in the control group, whereas it was widely and significantly expressed in the severe group. The control and the moderate groups had similar OPG levels, which were significantly higher than that in the severe group. Leptin was positively correlated with OPG(r = 0.905, < 0.01) and negatively correlated with RANKL (r = -0.635, < 0.01). low concentrations of leptin led to an increased / mRNA ratio, whereas the opposite effect was observed at high concentrations. Leptin can regulate OPG and RANKL expression in gingival fibroblasts and may thus play a role in the development of chronic periodontitis by modulating the ratio.
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http://dx.doi.org/10.7150/ijms.56151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100636PMC
April 2021

New aspects of microbial vitamin K2 production by expanding the product spectrum.

Microb Cell Fact 2021 Apr 13;20(1):84. Epub 2021 Apr 13.

Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Vitamin K2 (menaquinone, MK) is an essential lipid-soluble vitamin with critical roles in blood coagulation and bone metabolism. Chemically, the term vitamin K2 encompasses a group of small molecules that contain a common naphthoquinone head group and a polyisoprenyl side chain of variable length. Among them, menaquinone-7 (MK-7) is the most potent form. Here, the biosynthetic pathways of vitamin K2 and different types of MK produced by microorganisms are briefly introduced. Further, we provide a new aspect of MK-7 production, which shares a common naphthoquinone ring and polyisoprene biosynthesis pathway, by analyzing strategies for expanding the product spectrum. We review the findings of metabolic engineering strategies targeting the shikimate pathway, polyisoprene pathway, and menaquinone pathway, as well as membrane engineering, which provide comprehensive insights for enhancing the yield of MK-7. Finally, the current limitations and perspectives of microbial menaquinone production are also discussed. This article provides in-depth information on metabolic engineering strategies for vitamin K2 production by expanding the product spectrum.
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http://dx.doi.org/10.1186/s12934-021-01574-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042841PMC
April 2021

Porcine circovirus type 2 infection activates NF-κB pathway and cellular inflammatory responses through circPDCD4/miR-21/PDCD4 axis in porcine kidney 15 cell.

Virus Res 2021 Jun 10;298:198385. Epub 2021 Mar 10.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Porcine circovirus type 2 (PCV2) causes postweaning multisystemic wasting syndrome (PMWS) as well as other PCV-associated diseases (PCVADs), which seriously affect the development of the swine industry. The association between aberrant expression of microRNA-21 (miR-21) and the pathogenesis of inflammatory diseases is already known. Also, our previous study showed elevated ssc-miR-21-5p (miR-21) levels in PCV2-infected porcine kidney 15 (PK-15) cells. However, the functional expression of miR-21 in PCV2 infection is unknown. Here, we found that the miR-21 levels were substantially upregulated in PCV2-infected cells, while the expression of PDCD4 and a PDCD4-derived circRNA (circPDCD4) were downregulated compared with the noninfected cells. The results of RNA immunoprecipitation and dual-luciferase reporter (DLR) assay revealed that circPDCD4 acted as a miR-21 sponge and PDCD4 was a miR-21 target. Functionally, we found that miR-21 overexpression induced the NF-κB pathway along with inflammation in cells exposed to PCV2. Further, the overexpression of PDCD4 or circPDCD4 downregulated miR-21 levels and subsequently, attenuated the miR-21-induced activation of inflammation and the NF-κB pathway. Thus, PCV2 activated the NF-κB pathway and cellular inflammatory responses through regulating circPDCD4, miR-21, and PDCD4 in PK-15 cells.
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http://dx.doi.org/10.1016/j.virusres.2021.198385DOI Listing
June 2021

Inhibition of Porcine Epidemic Diarrhea Virus Replication and Viral 3C-Like Protease by Quercetin.

Int J Mol Sci 2020 Oct 30;21(21). Epub 2020 Oct 30.

State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, China.

For the last decade, porcine epidemic diarrhea virus (PEDV) variant strains have caused severe damage to the global pig industry. Until now, no effective antivirals have been developed for the therapeutic treatment of PEDV infection. In the present study, we found that quercetin significantly suppressed PEDV infection at noncytotoxic concentrations. A molecular docking study indicated that quercetin might bind the active site and binding pocket of PEDV 3C-like protease (3CL). Surface plasmon resonance (SPR) analysis revealed that quercetin exhibited a binding affinity to PEDV 3CL. Based on the results of the fluorescence resonance energy transfer (FRET) assay, quercetin was proven to exert an inhibitory effect on PEDV 3CL. Since coronavirus 3CL is an important drug target and participates in the viral replication process, quercetin should be developed as a novel drug in the control of PEDV infection.
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http://dx.doi.org/10.3390/ijms21218095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662296PMC
October 2020

Extraordinary optical transmission through periodic Drude-like graphene sheets using FDTD algorithms and its unconditionally stable approximate Crank-Nicolson implementation.

Sci Rep 2020 Oct 15;10(1):17462. Epub 2020 Oct 15.

Department of Electrical and Electronic Engineering, College of Engineering, Yantai Nanshan University, Longkou, 265713, China.

Based upon the approximate Crank-Nicolson (CN) finite-difference time-domain method implementation, the unconditionally stable algorithm is proposed to investigate the wave propagation and transmission through extremely thin graphene layers. More precisely, by incorporating the CN Douglas-Gunn algorithm, the piecewise linear recursive convolution method and the auxiliary differential equation method, the analytical model is proposed for Drude-like graphene model. To obtain the solution of the governing equations, the perfectly matched layer and the periodic boundary condition are applied to the graphene structure with two dimensional nano-materials. Numerical examples are carried out for further investigation. During the simulation, the influences of the parameters such as the grating slit and its thickness on the wave transmission are investigated and discussed. The result shows that not only the graphene grating has high transmission performance but also the proposed methods have considerable performance and accuracy.
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http://dx.doi.org/10.1038/s41598-020-74552-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566490PMC
October 2020

Diagnostic values of Xpert MTB/RIF, T-SPOT.TB and adenosine deaminase for HIV-negative tuberculous pericarditis in a high burden setting: a prospective observational study.

Sci Rep 2020 10 1;10(1):16325. Epub 2020 Oct 1.

Department of Medical Records, Henan Chest Hospital, Zhengzhou, Henan Province, People's Republic of China.

The diagnosis of tuberculous pericarditis (TBP) remains challenging. This prospective study evaluated the diagnostic value of Xpert MTB/RIF (Xpert) and T-SPOT.TB and adenosine deaminase (ADA) for TBP in a high burden setting. A total of 123 HIV-negative patients with suspected TBP were enrolled at a tertiary referral hospital in China. Pericardial fluids were collected and subjected to the three rapid tests, and the results were compared with the final confirmed diagnosis. Of 105 patients in the final analysis, 39 (37.1%) were microbiologically, histopathologically or clinically diagnosed with TBP. The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio (DOR) for Xpert were 66.7%, 98.5%, 96.3%, 83.3%, 44.0, 0.338, and 130.0, respectively, compared to 92.3%, 87.9%, 81.8%, 95.1%, 7.6, 0.088, and 87.0, respectively, for T-SPOT.TB, and 82.1%, 92.4%, 86.5%, 89.7%, 10.8, 0.194, and 55.8, respectively, for ADA (≥ 40 U/L). ROC curve analysis revealed a cut-off point of 48.5 spot-forming cells per million pericardial effusion mononuclear cells for T-SPOT.TB, which had a DOR value of 183.8, while a cut-off point of 41.5 U/L for ADA had a DOR value of 70.9. Xpert (Step 1: rule-in) followed by T-SPOT.TB [cut-off point] (Step 2: rule-out) showed the highest DOR value of 252.0, with only 5.7% (6/105) of patients misdiagnosed. The two-step algorithm consisting of Xpert and T-SPOT.TB could offer rapid and accurate diagnosis of TBP.
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http://dx.doi.org/10.1038/s41598-020-73220-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530650PMC
October 2020

Fixed-Time Fuzzy Control for a Class of Nonlinear Systems.

IEEE Trans Cybern 2020 Sep 23;PP. Epub 2020 Sep 23.

Fixed-time tracking control is considered for a class of nonlinear systems in this article. Different from the conventional literature on fixed-time control studies, in this article, the nonlinearities of systems are all completely unknown. Fuzzy-logic systems are utilized to model these unknown nonlinearities. To deal with the fixed-time control under the approximation errors, three steps are taken. First, a new criterion of fixed-time stability is developed; second, a new fixed-time control scheme is proposed, which is different from the existing adaptive design method; and third, to analyze the fixed-time stability of the system, two novel inequalities are established. It shows that the proposed fuzzy control scheme can guarantee system performance in a fixed time, and the upper bound of the settling time only depends on the design parameters.
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http://dx.doi.org/10.1109/TCYB.2020.3018695DOI Listing
September 2020

A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris.

J Ind Microbiol Biotechnol 2020 Dec 15;47(12):1161-1172. Epub 2020 Sep 15.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China.

Phytase is an additive in animal feed that degrades phytic acid in plant material, reducing feeding costs, and pollution from fecal phosphorus excretion. A multistrategy approach was adopted to improve the expression of E. coli phytase in Pichia pastoris. We determined that the most suitable signal peptide for phytase secretion was an α-factor secretion signal with an initial enzyme activity of 153.51 U/mL. Increasing the copy number of this gene to four increased phytase enzyme activity by 234.35%. PDI overexpression and Pep4 gene knockout increased extracellular phytase production by 35.33% and 26.64%, respectively. By combining favorable factors affecting phytase expression and secretion, the enzyme activity of the phytase-engineered strain was amplified 384.60% compared with that of the original strain. We also evaluated the potential for the industrial production of the engineered strain using a 50-L fed-batch fermenter and achieved a total activity of 30,246 U/mL after 180 h of fermentation.
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http://dx.doi.org/10.1007/s10295-020-02311-6DOI Listing
December 2020

Expression and methylation of Dickkopf-1 in the pathogenesis and malignant transformation of oral submucous fibrosis.

J Oral Pathol Med 2020 Sep 30;49(8):809-815. Epub 2020 Aug 30.

Department of Oral Mucosa, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China.

Background: Dickkopf-1 is an inhibitor of the Wnt/β-catenin pathway, but the role of Dickkopf-1 in oral submucous fibrosis remains unclear. We evaluated the protein expression and gene methylation levels of dickkopf-1 to determine the mechanism underlying abnormal Wnt/β-catenin pathway activation.

Methods: Healthy mucosa, oral submucous fibrosis, oral squamous cell carcinoma, and cancer-adjacent tissues were collected. The expression and promoter methylation levels of dickkopf-1 were analyzed.

Results: The expression levels of dickkopf-1 in oral submucous fibrosis and oral squamous cell carcinoma tissues were lower than those in healthy and cancer-adjacent tissues. The methylation levels of the dickkopf-1 gene in oral submucous fibrosis and oral squamous cell carcinoma tissues were higher than those in healthy and cancer-adjacent tissues. Dickkopf-1 expression was negatively correlated with dickkopf-1 gene methylation.

Conclusions: High dickkopf-1 methylation levels in oral submucous fibrosis and oral squamous cell carcinoma tissues may decrease dickkopf-1 expression, which may induce an abnormal activation of the Wnt/β-catenin pathway and oral submucous fibrosis pathogenesis.
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http://dx.doi.org/10.1111/jop.13096DOI Listing
September 2020

Topological Regularization of Networks in Adult Patients with Moderate-to-Severe Obstructive Sleep Apnea-Hypopnea Syndrome: A Structural MRI Study.

Nat Sci Sleep 2020 8;12:333-345. Epub 2020 Jun 8.

Department of Radiology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, People's Republic of China.

Objective: Patients with obstructive sleep apnea-hypopnea syndrome (OSAHS) exhibit neurocognitive impairments; however, the neuroimaging mechanism of neurocognitive impairments remains unclear. The aim of this study was to understand the neuroimaging mechanism in adult patients with moderate-to-severe OSAHS, from the perspective of the connectome.

Patients And Methods: Thirty-one untreated patients with moderate-to-severe OSAHS (mean age: 41.23±8.22) were compared with 26 good sleepers (GS) (mean age: 39.50±7.92) matched according to age, gender, handedness, and education level. All subjects underwent thin-slice T1WI scanning of the skull using a 3.0T MRI. Then, a large-scale structural covariance network was constructed based on the gray matter volume extracted from the structural MRI. Graph theory was then used to determine the topological changes in the structural covariance network of OSAHS patients.

Results: Although small-world networks were retained,the structural covariance network exhibited topological irregularities in regular architecture as evidenced by an increase in the clustering coefficient (=0.009), transfer coefficient (=0.029) and local efficiency (=0.031), and a local increase in the shortest path length (<0.05) compared with the GS group. Locally, OSAHS patients showed a decrease in nodal betweenness and degree in the left inferior parietal gyrus, left angular gyrus and right anterior cingulate cortex compared with the GS group (<0.05, uncorrected). In addition, the resistance of structural covariance networks in OSAHS patients to random fault is significantly lower than that of the GS group (=0.044).

Conclusion: Structural covariance networks are abnormal in terms of multiple network parameters, which provide network-level insight into the neuroimaging mechanism of cognitive impairments in adult OSAHS patients.
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http://dx.doi.org/10.2147/NSS.S248643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293417PMC
June 2020

Relationship Between Self-Care Behavior and Cognitive Function in Hospitalized Adult Patients with Type 2 Diabetes: A Cross-Sectional Study.

Diabetes Metab Syndr Obes 2020 29;13:207-214. Epub 2020 Jan 29.

Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing 100191, People's Republic of China.

Purpose: To investigate the relationship between diabetes self-care behavior and cognitive function of hospitalized young and middle-aged Chinese patients with type 2 diabetes mellitus (T2DM).

Patients And Methods: In this cross-sectional study, young and middle-aged T2DM patients (age range, 35-65 years) were recruited at 4 tertiary hospitals between July 2016 and January 2017. Data pertaining to self-care behavior and cognitive function were collected using two questionnaires (the Summary of Diabetes Self-care Activities [SDSCA] and the Montreal Cognitive Assessment [MoCA], respectively). Multivariate linear regression analysis was performed to assess the correlation between cognitive function and self-care activities.

Results: A total of 140 patients with diabetes were enrolled (mean age, 53.79±7.96 years). The mean duration of T2DM was 10.83±6.76 years. Regarding SDSCA performance, the mean scores for foot care and blood glucose monitoring were 2.20±2.57 and 1.98±2.45, respectively, which were the worst; scores for exercise (4.01±2.58) and diet (3.16±1.89) were better, while scores for medication administration (5.26±2.79) were the best. The prevalence of cognitive impairment was 37.9% (53 patients). After variables adjustment, delayed recall showed a significant correlation with blood sugar monitoring behavior ( =0.224, P=0.019); visual space and executive function (=0.255, =0.009) and abstraction (=-0.337, =0.001) showed a correlation with foot care behavior.

Conclusion: Cognitive ability affects the self-care behavior of patients with T2DM. Assessment of cognitive function may help inform patient education interventions to improve the self-care behavior of these patients.
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http://dx.doi.org/10.2147/DMSO.S236966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996216PMC
January 2020

Beer-spoilage characteristics of newly isolated from craft beer and its potential to influence beer quality.

Food Sci Nutr 2019 Dec 15;7(12):3950-3957. Epub 2019 Nov 15.

School of Biological Engineering Dalian Polytechnic University Dalian China.

To meet demands for fresh flavor and unique taste from beer consumer, there is an increase in the popularity of craft beer, which is more susceptible to microbial contamination than the industry beer. A beer-spoilage strain was isolated from craft beer and identified as strain BS7. The isolate BS7 showed that high beer-spoilage ability at low temperature (4°C), low pH (4.0) and high ethanol concentration (7.0%, v/v). Compared with the other known strains of , strain BS7 was resistant to hop compounds and had an evolutionary stability in hop resistance. Strain BS7 was able to grow quickly and utilizes nutrients in commercial beer, produces organic acids and biogenic amines, and changes beer flavor profile. These results suggest that strain BS7 is a beer-spoilage strain with the danger, which can lead to the beer-spoilage issues during craft beer production.
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http://dx.doi.org/10.1002/fsn3.1256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924307PMC
December 2019

PCV2 Regulates Cellular Inflammatory Responses through Dysregulating Cellular miRNA-mRNA Networks.

Viruses 2019 11 13;11(11). Epub 2019 Nov 13.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430000, China.

Porcine circovirus type 2 (PCV2) is closely linked to postweaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases (PCVADs), which influence the global pig industry. MicroRNAs (miRNAs) are evolutionarily conserved classes of endogenous small non-coding RNA that regulate almost every cellular process. According to our previous transcription study, PCV2 infection causes up-regulation of genes related to inflammation. To reveal the function of miRNAs in PCV2 infection and PCV2-encoded miRNAs, next generation sequencing and data analysis was performed to explore miRNA expression in PCV2-infected cells and non-infected cells. Data analysis found some small RNAs matched the PCV2 genome but PCV2 does not express miRNAs in an in vitro infection (PK-15 cells). More than 297 known and 427 novel miRNAs were identified, of which 44 miRNAs were differently expressed (DE). The pathways of inflammation mediated by chemokine and cytokine signaling pathway (P00031), were more perturbed in PCV2-infected cells than in mock controls. DE miRNAs and DE mRNA interaction network clearly revealed that PCV2 regulates the cellular inflammatory response through dysregulating the cellular miRNA-mRNA network. MiRNA overexpression and down-expression results demonstrated that miRNA dysregulation could affect PCV2 infection-induced cellular inflammatory responses. Our study revealed that host miRNA can be dysregulated by PCV2 infection and play an important role in PCV2-modulated inflammation.
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http://dx.doi.org/10.3390/v11111055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893612PMC
November 2019

Porcine antiviral activity is increased by CRISPRa-SAM system.

Biosci Rep 2019 08 19;39(8). Epub 2019 Aug 19.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China

Clustered Regularly Interspaced Short Palindromic Repeat activation-synergistic activation mediator system (CRISPRa-SAM) has been efficiently used to up-regulate the targeted genes in human and mouse. But it is not known whether the CRISPRa-SAM system can be used against porcine disease because its two important transcriptional activation domains (P65 and heat shock transcription factor 1 (HSF1)) are from mouse and human, respectively. Pig is one of the most important meat sources, porcine viral infectious diseases cause massive economic losses to the swine industry and threaten the public health. We aimed to investigate whether the CRISPRa-SAM system could increase porcine antiviral activity by mediating two pig-specific target genes ( and β1,4 N-acetylgalactosaminyltransferase ()). First, we constructed PK-15 and IPEC-J2 cell lines expressing nuclease-deficient Cas9 (dCas9)-vp64 and MS2-P65-HSF1 stably. Next, in these two cell models, we activated and expression through CRISPRa-SAM system. Antiviral activity to PRV or H9N2 was improved in PK-15 cells where or was activated. Altogether, our results demonstrated the potential of CRISPRa-SAM system as a powerful tool for activating pig genes and improving porcine antiviral activity.
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http://dx.doi.org/10.1042/BSR20191496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702355PMC
August 2019

Finite-Time Fuzzy Control of Stochastic Nonlinear Systems.

IEEE Trans Cybern 2019 Jul 18. Epub 2019 Jul 18.

This paper studies the finite-time stabilization of a class of stochastic nonlinear systems. Different from functions which are necessarily known in the conventional finite-time control of nonlinear systems, the nonlinear functions can be completely unknown in this paper. By applying fuzzy-logic systems to approximate the unknown nonlinearities, a novel adaptive finite-time control strategy is proposed. However, due to the existence of approximation errors, the existing finite-time stability criterion cannot be used to analyze the stability of stochastic nonlinear systems. To deal with this difficulty, a finite-time stability criterion, by utilizing the mean value theorem of integrals, is first established in Lemma 5, which plays a significant role in the finite-time stability analysis of stochastic nonlinear systems. Then, the finite-time mean square stability of a stochastic nonlinear system is proved by combining Lemma 3 with Jensen's inequality.
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http://dx.doi.org/10.1109/TCYB.2019.2925573DOI Listing
July 2019

Study on the prediction of the contamination symptoms in the fermentation process of Chlortetracycline based on soft sensor modeling method.

Technol Health Care 2019 ;27(S1):205-215

College of Electronic Engineering, Yantai Nanshan University, Longkou, Shandong 265713, China.

Background: How to accurately predict the occurrence of contamination in the fermentation process of Chlortetracycline? How to prompt field operators to take effective measures in time? This is a difficult problem that the fermentation process of Chlortetracycline has not been solved well.

Objective: The aim of this paper is to effectively predict whether the fermentation process of Chlortetracycline is contaminated or not.

Methods: A Gaussian process regression soft sensor modeling method with real time integration learning is studied in depth by combining two local learning strategies, namely just-in-time learning (JITL) method and integrated learning method, and a multi-model weighted Gaussian process regression (MWGPR) soft sensor modeling method based on real-time integration learning is proposed in the paper. This soft sensing method was used to study the relationship between the viscosity of fermentation broth and the contamination in fermentation process. A soft-sensing model based on the viscosity of fermentation broth for predicting the signs of contamination is established.

Results: The validity of this method is verified by field data. The experimental results demonstrate that the soft sensing model proposed in this paper can effectively determine whether the fermentation broth is infected by hybrid bacteria.

Conclusions: The method proposed in this paper is innovative and practical so that field operators can issue early warning and take effective measures.
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http://dx.doi.org/10.3233/THC-199020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597973PMC
January 2020

Irregular structural networks of gray matter in patients with type 2 diabetes mellitus.

Brain Imaging Behav 2020 Oct;14(5):1477-1486

Department of MRI, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Type 2 diabetes mellitus (T2DM) induces dementia and cognitive decrements indicating the impairment of the central nervous system. While there is evidence showing abnormalities in white-matter structural networks in T2DM, the topological features of gray matter are still unknown. The study enrolled 30 right-handed T2DM patients and 20 healthy control subjects with matched age, gender, handedness, and education. Graph theoretical analysis of magnetic resonance imaging on gray matter volume was conducted to explore large-scale structural networks of brain. Although retaining small-worldness characteristics, the structural networks of grey matter in the T2DM group exhibited an increased clustering coefficient, prolonged characteristic path, decreased global efficiency, and more vulnerability to random failures or targeted attacks compared with controls. Additionally, the degree of structural networks in both T2DM and control groups was distributed exponentially in truncated power law. Our findings suggest that T2DM disturbed the overall topological features of gray matter networks, which provides a novel insight into the neurobiological mechanisms accounting for the cognitive impairment of T2DM patients.
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http://dx.doi.org/10.1007/s11682-019-00070-2DOI Listing
October 2020

Evolution of E. coli Phytase for Increased Thermostability Guided by Rational Parameters.

J Microbiol Biotechnol 2019 Mar;29(3):419-428

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, P.R. China.

Phytases are enzymes that can hydrolyze phytate and its salts into inositol and phosphoric acid, and have been utilized to increase the availability of nutrients in animal feed and mitigate environmental pollution. However, the enzymes’low thermostability has limited their application during the feed palletization process. In this study, a combination of B-value calculation and protein surface engineering was applied to rationally evolve the heat stability of phytase. After systematic alignment and mining for homologs of the original phytase from the histidine acid phosphatase family, the two models 1DKL and 1DKQ were chosen and used to identify the B-values and spatial distribution of key amino acid residues. Consequently, thirteen potential amino acid mutation sites were obtained and categorized into six domains to construct mutant libraries. After five rounds of iterative mutation screening, the thermophilic phytase mutant P56214 was finally yielded. Compared with the wild-type, the residual enzyme activity of the mutant increased from 20% to 75% after incubation at 90°C for 5 min. Compared with traditional methods, the rational engineering approach used in this study reduces the screening workload and provides a reference for future applications of phytases as green catalysts.
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http://dx.doi.org/10.4014/jmb.1811.11017DOI Listing
March 2019

A simple pyramid-shaped microchamber towards highly efficient isolation of circulating tumor cells from breast cancer patients.

Biomed Microdevices 2018 09 17;20(4):83. Epub 2018 Sep 17.

College of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan, Hubei, 430200, People's Republic of China.

Isolation and detection of circulating tumor cells (CTCs) has showed a great clinical impact for tumor diagnosis and treatment monitoring. Despite significant progresses of the existing technologies, feasible and cost-effective CTC isolation techniques are more desirable. In this study, a novel method was developed for highly efficient isolation of CTCs from breast cancer patients based on biophysical properties using a pyramid-shaped microchamber. Through optimization tests, the outlet height of 6 μm and the flow rate of 200 μL/min were chosen as the optimal conditions. The capture efficiencies of more than 85% were achieved for cancer cell lines (SKBR3, BGC823, PC3, and H1975) spiked in DMEM and healthy blood samples without clogging issue. In clinic assay, the platform identified CTCs in 13 of 20 breast cancer patients (65%) with an average of 4.25 ± 4.96 CTCs/2 mL, whereas only one cell was recognized as CTC in 1 of 15 healthy blood samples. The statistical analyses results demonstrated that both CTC positive rate and CTC counts were positive correlated with TNM stage (p < 0.001; p = 0.02, respectively). This microfluidic platform successfully demonstrated the clinical feasibility of CTC isolation and would hold great potential of clinical application in predicting and monitoring the prognosis of cancer patients.
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http://dx.doi.org/10.1007/s10544-018-0326-0DOI Listing
September 2018

Psychological Status of Volunteers in a Phase I Clinical Trial Assessed by Symptom Checklist 90 (SCL-90) and Eysenck Personality Questionnaire (EPQ).

Med Sci Monit 2018 Jul 17;24:4968-4973. Epub 2018 Jul 17.

Peking University School of Nursing, Beijing, China (mainland).

BACKGROUND The psychological status of volunteers was investigated to provide a theoretical method for Phase I clinical trial management and result analysis. MATERIAL AND METHODS The Symptom Checklist 90 (SCL-90) and Eysenck Personality Questionnaire (EPQ) were used to assess the psychological status 200 healthy Chinese volunteers. RESULTS SCL-90 results indicate that the average value of positive factors is 10.32±14.26 by self-assessment of healthy volunteers, somatization factor is 1.13±0.13, compulsive symptom factor is 1.29±0.27, interpersonal sensitivity factor is 1.31±0.21, depression factor is 1.26±0.33, anxiety factor is 1.21±0.21, hostility factor is 1.08±0.26, phobia factor is 1.05±0.18, paranoid factor is 1.12±0.23, and psychotic symptom factor is 1.17±0.26. CONCLUSIONS Compared to the norm in China, the score of each factor of healthy volunteers was relatively low, with a statistically significant difference (P<0.001). EPQ results show that P score was 4.59±2.33, E score is 13.13±4.32, N score was 6.89±5.26, and L score was 13.21±4.25 for the 200 healthy volunteers. Compared to the norm in China, the P and N scores were lower, and the E and L scores were higher, with a statistically significant difference (P<0.001).
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http://dx.doi.org/10.12659/MSM.909524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6067027PMC
July 2018

Bone marrow-derived inflammatory and steady state DCs are different in both functions and survival.

Cell Immunol 2018 09 7;331:100-109. Epub 2018 Jun 7.

Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China. Electronic address:

Dendritic cells (DCs) contain heterogeneous populations, with classical DCs developed at steady state and monocyte-derived DCs mobilized under inflammatory conditions, although their total numbers in vivo are scares. To obtain enough quantity for immunological study or clinical application, we have previously established that bone marrow-derived DCs in the presence of Flt-3L (FL-DCs) or GM-CSF (GM-DCs) in vitro are equivalent to the steady state DCs and inflammatory DCs in vivo respectively. What difference, however, exists between these two most commonly used culture systems in DC functions and survival, and how does it correlate to the division of works by their corresponding counterparts in vivo remain ill-defined. In this study, we found that GM-DCs of inflammatory nature were more phagocytic, potent at inducing Th2 and Th17 differentiation, and had longer survival rate, whereas FL-DCs of steady state characters were stronger T cell activator and better at directing Th1 differentiation. Mechanistically, NO production induced by the LPS-activated GM-DCs could partly explain for their failure to improve T cell proliferation, and the distinct T cell differentiation profiles and viability demonstrated by the two types of DCs were underpinned by their preferential secretion of T cell polarizing cytokines and expression of anti-apoptotic genes. Such disparate functionalities and survival potentials of steady state and inflammatory DCs in vitro fit in well with their respective roles in vivo in particular immunological settings and have serious implications in translational applications.
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http://dx.doi.org/10.1016/j.cellimm.2018.06.001DOI Listing
September 2018

Enhancing the production of phenolic compounds during barley germination by using chitooligosaccharides to improve the antioxidant capacity of malt.

Biotechnol Lett 2018 Oct 6;40(9-10):1335-1341. Epub 2018 Jun 6.

School of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, People's Republic of China.

Objective: To enhance the production of phenolic compounds during barley germination using chitooligosaccharide as an elicitor to improve the antioxidant capacity of malt.

Results: When used as an elicitor for barley germination, chitooligosaccharide with a molecular weight of 3 kDa, added at 10 mg/kg barley kernels during the first steeping cycle, led to the maximum production of phenolic compounds. Compared with the control with no chitooligosaccharide added to the steeping water, the total phenolic content was increased by 54.8%. Increases in the total phenolic content of the barley malt occurred when chitooligosaccharide was applied during the first or both the first and the second steeping cycles. Thus the antioxidant capacity of barley malt was increased significantly by adding chitooligosaccharide during the steeping process.

Conclusion: Applying chitooligosaccharides during the steeping process increased the content of phenolic compounds thus improving the antioxidant capacity of the barley malt.
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http://dx.doi.org/10.1007/s10529-018-2582-8DOI Listing
October 2018

Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication.

Viruses 2018 03 13;10(3). Epub 2018 Mar 13.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.
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http://dx.doi.org/10.3390/v10030127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869520PMC
March 2018

Finite-Time Adaptive Control for a Class of Nonlinear Systems With Nonstrict Feedback Structure.

IEEE Trans Cybern 2018 Oct 18;48(10):2774-2782. Epub 2017 Sep 18.

This paper focuses on finite-time adaptive neural tracking control for nonlinear systems in nonstrict feedback form. A semiglobal finite-time practical stability criterion is first proposed. Correspondingly, the finite-time adaptive neural control strategy is given by using this criterion. Unlike the existing results on adaptive neural/fuzzy control, the proposed adaptive neural controller guarantees that the tracking error converges to a sufficiently small domain around the origin in finite time, and other closed-loop signals are bounded. At last, two examples are used to test the validity of our results.
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http://dx.doi.org/10.1109/TCYB.2017.2749511DOI Listing
October 2018

A flow cytometry-based screen identifies MBNL1 modulators that rescue splicing defects in myotonic dystrophy type I.

Hum Mol Genet 2017 08;26(16):3056-3068

Rare Disease Research Unit.

Myotonic dystrophy Type 1 (DM1) is a rare genetic disease caused by the expansion of CTG trinucleotide repeats ((CTG)exp) in the 3' untranslated region of the DMPK gene. The repeat transcripts sequester the RNA binding protein Muscleblind-like protein 1 (MBNL1) and hamper its normal function in pre-mRNA splicing. Overexpressing exogenous MBNL1 in the DM1 mouse model has been shown to rescue the splicing defects and reverse myotonia. Although a viable therapeutic strategy, pharmacological modulators of MBNL1 expression have not been identified. Here, we engineered a ZsGreen tag into the endogenous MBNL1 locus in HeLa cells and established a flow cytometry-based screening system to identify compounds that increase MBNL1 level. The initial screen of small molecule compound libraries identified more than thirty hits that increased MBNL1 expression greater than double the baseline levels. Further characterization of two hits revealed that the small molecule HDAC inhibitors, ISOX and vorinostat, increased MBNL1 expression in DM1 patient-derived fibroblasts and partially rescued the splicing defect caused by (CUG)exp repeats in these cells. These findings demonstrate the feasibility of this flow-based cytometry screen to identify both small molecule compounds and druggable targets for MBNL1 upregulation.
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http://dx.doi.org/10.1093/hmg/ddx190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886090PMC
August 2017

The use of chitooligosaccharide in beer brewing for protection against beer-spoilage bacteria and its influence on beer performance.

Biotechnol Lett 2016 Apr 14;38(4):629-35. Epub 2015 Dec 14.

School of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, People's Republic of China.

Objectives: To identify a biological preservative that can protect beer from microbial contamination, which often results in the production of turbidity and off-flavor.

Results: The antimicrobial activity of a chitooligosaccharide against beer-spoilage bacteria and its effect on the fermentation performance of brewer's yeast was studied. Chitooligosaccharide with an average 2 kDa molecular weight was the best at inhibiting all tested beer-spoilage bacteria. The application of chitooligosaccharide in the brewing process did not influence the fermentation of brewer's yeast. The change in beer performance induced by the contamination of Lactobacillus brevis could be effectively controlled by application of chitooligosaccharide in the beer brewing process.

Conclusion: The experimental data suggested that chitooligosaccharide should be an excellent preservative to inhibit beer-spoilage bacteria in the brewing process and in the end product.
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http://dx.doi.org/10.1007/s10529-015-2013-zDOI Listing
April 2016

Characteristics of Newly Isolated Geobacillus sp. ZY-10 Degrading Hydrocarbons in Crude Oil.

Pol J Microbiol 2015 ;64(3):253-63

An obligately thermophilic strain ZY-10 was isolated from the crude oil in a high-temperature oilfield, which was capable of degrading heavy crude oil. Phenotypic and phylogenetic analysis demonstrated that the isolate should be grouped in the genus Geobacillus, which shared thd highest similarity (99%) of the 16S rDNA sequence to Geobacillus stearothermophilus. However, the major cellular fatty acid iso-15:0 (28.55%), iso-16:0 (24.93%), iso-17:0 (23.53%) and the characteristics including indole production, tolerance to NaN3 and carbohydrate fermentation showed some difference from the recognized species in the genus Geobacillus. The isolate could use tridecane, hexadecane, octacosane and hexatridecane as sole carbon source for cell growth, and the digesting rate of long-chain alkane was lower than that of short-chain alkane. When the isolate was cultured in the heavy crude oil supplement with inorganic salts and trace yeast extract, the concentration of short-chain alkane was significantly increased and the content of long-chain alkane was decreased, suggesting that the larger hydrocarbon components in crude oil were degraded into shorter-chain alkane. Strain ZY-10 would be useful for improving the mobility of crude oil and upgrading heavy crude oil in situ.
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February 2016
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