Publications by authors named "Yumei Qin"

23 Publications

  • Page 1 of 1

A nitric oxide-eluting and REDV peptide-conjugated coating promotes vascular healing.

Biomaterials 2022 05 28;284:121478. Epub 2022 Mar 28.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610065, China. Electronic address:

Drug-eluting stents (DESs) placement remarkably reduces the over-proliferation of smooth muscle cells (SMCs) and thus neointimal hyperplasia. However, the pharmacological agent also slows down the re-endothelization, delays injury vascular healing and increases the risk of in-stent restenosis (ISR). Here, inspired by mussel foot proteins (Mfps), a mimicking endothelium functional stent coating was efficiently fabricated by thiol-ene "click" reaction, consisting of catechol grafted chitosan (CS-C), zinc sulfate, and Arg-Glu-Asp-Val (REDV) peptide. The mimicking endothelium coating could continuously catalyze endogenous nitric oxide (NO) gas and maintain the bioactivity of REDV peptide. Compared with bare stents, the mimicking coatings significantly inhibited the acute thrombosis for the first 1-week, accelerated re-endothelization and decreased in-stent restenosis for 1- and 3-month after implantation. In addition, the synergistic effect of NO and REDV peptide also regulated inflammation response and promoted the expression of muscle fiber.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121478DOI Listing
May 2022

A Polyphenol-Network-Mediated Coating Modulates Inflammation and Vascular Healing on Vascular Stents.

ACS Nano 2022 Mar 18. Epub 2022 Mar 18.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610065, China.

Localized drug delivery from drug-eluting stents (DESs) to target sites provides therapeutic efficacy with minimal systemic toxicity. However, DESs failure may cause thrombosis, delay arterial healing, and impede re-endothelialization. Bivalirudin (BVLD) and nitric oxide (NO) promote arterial healing. Nevertheless, it is difficult to combine hydrophilic signal molecules with hydrophobic antiproliferative drugs while maintaining their bioactivity. Here, we fabricated a micro- to nanoscale network assembly consisting of copper ion and epigallocatechin gallate (EGCG) π-π interactions, metal coordination, and oxidative polymerization. The network incorporated rapamycin and immobilized BVLD by the thiol-ene "click" reaction and provided sustained rapamycin and NO release. Unlike rapamycin-eluting stents, those coated with the EGCG-Cu-rapamycin-BVLD complex favored competitive endothelial cell (EC) growth over that of smooth muscle cells, exhibited long-term antithrombotic efficacy, and attenuated the negative impact of rapamycin on the EC. stent implantation demonstrated that the coating promoted endothelial regeneration and hindered restenosis. Therefore, the polyphenol-network-mediated surface chemistry can be an effective strategy for the engineering of multifunctional surfaces.
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http://dx.doi.org/10.1021/acsnano.2c00642DOI Listing
March 2022

Natural biopolymer masks the bitterness of potassium chloride to achieve a highly efficient salt reduction for future foods.

Biomaterials 2022 04 4;283:121456. Epub 2022 Mar 4.

Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Potassium chloride (KCl) can be considered as the most ideal salt replacer to reduce dietary sodium intake and ease various health risks of a high-sodium diet. However, a high proportion of sodium chloride (NaCl) replacement with KCl remains a challenge, because KCl has an inherent metallic bitterness. This study demonstrates a strategy for this bitterness-masking using a natural polysaccharide kappa-carrageenan to specifically bind with K and reduce the amount of free K as bitter stimulant. The results show that carrageenan can significantly slow down the release and diffusion of K, leading to a reduced bitter taste of KCl in the mouth. Up to 50% replacement of NaCl by KCl can be achieved. Furthermore, the use of carrageenan-KCl-NaCl complex as salt substitutes can regulate mineral absorption (Na, K, Ca) and reduce hypertension and renal injury risks in the animal tests. In conclusion, this natural biopolymer-based strategy successfully masks the bitter of salt-replacer KCl, opening a route to the universally applicable salt-reduction in future foods.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121456DOI Listing
April 2022

A targeted and nontargeted metabolomics study on the oral processing of epicatechins from green tea.

Food Chem 2022 Jun 11;378:132129. Epub 2022 Jan 11.

Tea Research Institute Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, 9 South Meiling Road, Hangzhou 310008, China. Electronic address:

Oral processing (OP), referring to the whole process of food digestion in human mouth, has a major influence on food flavor perception. This study focused on the compositional changes of the four green tea epicatechins (viz., EC, EGC, ECG, EGCG) during OP, based on targeted and nontargeted metabolomics. It was found that the four epicatechins were all extensively lost through transformation undergoing OP, among which EC was the most stable one, whereas EGCG the least. EGCG was further revealed to be susceptible to human oral cavity in the simulated OP in vitro. It could be converted physically by precipitating with mucin in saliva, and chemically through hydrolysis and dimerization, mediated mainly by the neutral pH condition. The OP of epicatechins also caused salivary composition changes possibly involving health benefits of green tea. These findings could raise awareness of the interactions between epicatechins, or any other food materials, with human mouth.
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http://dx.doi.org/10.1016/j.foodchem.2022.132129DOI Listing
June 2022

Reciprocal signals between nerve and epithelium: how do neurons talk with epithelial cells?

Am J Stem Cells 2021 15;10(4):56-67. Epub 2021 Oct 15.

Central Lab of Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University Hangzhou 310020, Zhejiang, China.

Most epithelium tissues continuously undergo self-renewal through proliferation and differentiation of epithelial stem cells (known as homeostasis), within a specialized stem cell niche. In highly innervated epithelium, peripheral nerves compose perineural niche and support stem cell homeostasis by releasing a variety of neurotransmitters, hormones, and growth factors and supplying trophic factors to the stem cells. Emerging evidence has shown that both sensory and motor nerves can regulate the fate of epithelial stem cells, thus influencing epithelium homeostasis. Understanding the mechanism of crosstalk between epithelial stem cells and neurons will reveal the important role of the perineural niche in physiological and pathological conditions. Herein, we review recent discoveries of the perineural niche in epithelium mainly in tissue homeostasis, with a limited touch in wound repair and pathogenesis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610808PMC
October 2021

In situ analysis of copper speciation during in vitro digestion: Differences between copper in drinking water and food.

Food Chem 2022 Mar 13;371:131388. Epub 2021 Oct 13.

Food Nutrition Science Centre, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China. Electronic address:

In recent years, the safety of copper in drinking water has increasingly been questioned. Copper speciation is an important factor that affects its bioavailability and toxicity; thus, it is critical to investigate the speciation of copper that is ingested from food and drinking water during in vitro digestion. After digestion, water- and food-derived copper formed 60 ± 4% 0.1-1 kDa and 49 ± 6% 10-1,000 kDa copper complexes, respectively. Under simulated fasting drinking water conditions, up to 90 ± 2% 0.1-1 kDa copper complexes formed. In addition, using ion selective electrode analysis, water-derived copper was detected that contained higher Cu concentrations after digestion than those of food-derived copper. These results indicate that water-derived copper forms smaller-sized species and exhibits higher Cu concentrations during digestion than those of food-derived copper, thereby highlighting the importance of reassessing the safety limit for copper in drinking water.
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http://dx.doi.org/10.1016/j.foodchem.2021.131388DOI Listing
March 2022

Design, synthesis, and biological evaluation of novel triazoloquinazolinone derivatives as SHP2 protein inhibitors.

J Enzyme Inhib Med Chem 2021 Dec;36(1):2170-2182

School of Pharmaceutical Sciences, Guizhou University, Guiyang, China.

A novel series of triazoloquinazolinone derivatives were designed, synthesised, and evaluated for their biological activities against the SHP2 protein. Moreover, some compounds were evaluated against A375 cells. The results revealed that target compounds possessed moderate to excellent inhibitory activity against SHP2 protein, whereas compounds , , , , and have strong antiproliferative activity on A375 cells. The compound showed remarkable cytotoxicity against A375 cells and a strong inhibitory effect against SHP2 protein when compared with . The structure-activity relationships (SARs) indicated that electron-donating groups (EDGs) on phenyl rings are beneficial for improving the antitumor activity; compounds with a hydroxyl substituent at the 2-position of phenyl ring exhibited superior activities than compounds with a substituent at the 4-position. In addition, compound displayed improved physicochemical properties as well as metabolic stability compared to . Our efforts identified as a promising SHP2 protein inhibitor, warranting its further investigation.
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http://dx.doi.org/10.1080/14756366.2021.1986491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583937PMC
December 2021

The influence of gastrointestinal pH on speciation of copper in simulated digestive juice.

Food Sci Nutr 2021 Sep 26;9(9):5174-5182. Epub 2021 Jul 26.

Food Safety Key Laboratory of Zhejiang Province School of Food Science and Biotechnology Zhejiang Gongshang University Hangzhou China.

Speciation can provide knowledge about absorption, reactivity to binding sites, bioavailability, toxicity, and excretion of elements. In this study, the speciation of copper in different model solutions under the influence of gastrointestinal (GI) pH was studied by ion selective electrode (ISE) and inductively coupled plasma optical emission spectrometry (ICP OES). It was found that the electrode response (mV) against Cu decreased with the increase in pH and dropped to the lowest point at pH 7.5 in all model solutions. When amino acids and organic acids were present, the ratio of filtered copper (0.45 μm, pH 7.5) was more than 90%. When casein was present, whey protein, pancreatin, and starch were added, and the ratio of filtered copper was 85.6 ± 0.3, 56.7 ± 8.8, 38.5 ± 5.1, and 1.0 ± 0.3%, respectively. When there is not enough organic ligand, excessive copper will form copper hydroxide precipitation with the increase in pH, but it got the highest electrode response (mV) against Cu. From this study, it can be concluded that the speciation of copper in GI tract is strongly influenced by the pH and the composition of food. When there are few ligands coexisting in the GI tract, the concentration of copper ion may be relatively high.
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http://dx.doi.org/10.1002/fsn3.2490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441336PMC
September 2021

Nkx2-2 expressing taste cells in endoderm-derived taste papillae are committed to the type III lineage.

Dev Biol 2021 09 5;477:232-240. Epub 2021 Jun 5.

Monell Chemical Senses Center, Philadelphia, PA, USA.

In mammals, multiple cell-signaling pathways and transcription factors regulate development of the embryonic taste system and turnover of taste cells in the adult stage. Using single-cell RNA-Seq of mouse taste cells, we found that the homeobox-containing transcription factor Nkx2-2, a target of the Sonic Hedgehog pathway and a key regulator of the development and regeneration of multiple cell types in the body, is highly expressed in type III taste cells but not in type II or taste stem cells. Using in situ hybridization and immunostaining, we confirmed that Nkx2-2 is expressed specifically in type III taste cells in the endoderm-derived circumvallate and foliate taste papillae but not in the ectoderm-derived fungiform papillae. Lineage tracing revealed that Nkx2-2-expressing cells differentiate into type III, but not type II or type I cells in circumvallate and foliate papillae. Neonatal Nkx2-2-knockout mice did not express key type III taste cell marker genes, while the expression of type II and type I taste cell marker genes were unaffected in these mice. Our findings indicate that Nkx2-2-expressing cells are committed to the type III lineage and that Nkx2-2 may be critical for the development of type III taste cells in the posterior tongue, thus illustrating a key difference in the mechanism of type III cell lineage specification between ectoderm- and endoderm-derived taste fields.
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http://dx.doi.org/10.1016/j.ydbio.2021.05.020DOI Listing
September 2021

Chronic administration of caffeine alters acesulfame-K intake and features of fungiform taste buds in mice.

Int J Food Sci Nutr 2021 Dec 4;72(8):1046-1056. Epub 2021 Apr 4.

Food Safety Laboratory, School of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou, China.

The objective of this study was to investigate the effects of chronic administration of caffeine on the anatomical characteristics of taste buds, the expression level of taste receptor protein in mice, and preference for a palatable solution. We found that following a 21-day administration of caffeine, mice showed increased behavioural responses to sweet stimuli (acesulfame-K solution). Mirroring this behavioural change, chronic caffeine treatment evidently decreased the maximal cross-sectional area and height of the longitudinal axis of fungiform taste buds, the number of taste cells per fungiform taste bud, and the expression of G protein α-gustducin, while the expression of the sweet taste receptors T1R2 and T1R3 was reversed. Our findings demonstrate that chronic administration of caffeine has an impact on taste sensitivity and changes in taste bud features, which may contribute to the alteration of taste behaviour.
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http://dx.doi.org/10.1080/09637486.2021.1905783DOI Listing
December 2021

An optimized organic acid human sensory sourness analysis method.

J Sci Food Agric 2021 Nov 23;101(14):5880-5887. Epub 2021 Apr 23.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Zhejiang, China.

Background: Sour taste perception builds on both chemical and physiological foundations, and plays an important role in food flavor, including that of fruit, beer, wine, and other beverages. A uniform sourness standard and sourness conversion method for researchers and food enterprises is necessary to obtain uniform conclusions.

Results: This study established an optimized organic acid sensory sourness analysis and sourness conversion method. It is based on sour sensory difference strength curves, which consist of an absolute threshold value and sensory difference threshold values. Defining the absolute threshold value of citric acid sourness as 1, sourness could be calculated according to the curve. With a logarithmic curve form, the acid sourness indexes (AI) were calculated as 1, 0.74, 0.77, 1.31, and 1.21 for citric, malic, fumaric, lactic, and tartaric acid samples, respectively. Consequently, each acid's sourness and concentration could be obtained and converted. Single acid and mixed acid sourness comparison evaluation's result implied that the novel method was more accurate (91.7-100%) than the hydrogen ion concentration method.

Conclusion: The novel sourness determination and conversion equation would provide more accurate sourness standard and calculation method in food sensory areas. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11240DOI Listing
November 2021

Age-Dependent Activation and Neuronal Differentiation of Lgr5+ Basal Cells in Injured Olfactory Epithelium Notch Signaling Pathway.

Front Aging Neurosci 2020 17;12:602688. Epub 2020 Dec 17.

School of Life Sciences, Shanghai University, Shanghai, China.

Aging is an important factor affecting function of smell, leading to the degeneration of mature olfactory sensory neurons and inducing the occurrence of smell loss. The mammalian olfactory epithelium (OE) can regenerate when subjected to chemical assaults. However, this capacity is not limitless. Inactivation of globose basal cells and failure to generate sensory neurons are the main obstacles to prevent the OE regeneration. Here, we found the significant attenuation in mature sensory neuronal generation and apparent transcriptional alternation in the OE from aged mice compared with young ones. The recruitment of leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5)-positive cells in injured OE was weakened in aged mice, and more Lgr5+ cells remained quiescence in aged OE postinjury. Lineage-traced progenies from Lgr5+ cells were significantly fewer in the OE with aging. Moreover, Notch activation enhanced the neuronal regeneration in aged OE, making the regenerative capacity of aged OE comparable with that of young animals after injury. The growth and morphology of three-dimensional (3D)-cultured organoids from the OE of young and aged mice varied and was modulated by small molecules regulating the Notch signaling pathway. Thus, we concluded that activation of Lgr5+ cells in injured OE was age dependent and Notch activation could enhance the capacity of neuronal generation from Lgr5+ cells in aged OE after injury.
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http://dx.doi.org/10.3389/fnagi.2020.602688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773941PMC
December 2020

Effects of sunset yellow on proliferation and differentiation of intestinal epithelial cells in murine intestinal organoids.

J Appl Toxicol 2021 06 15;41(6):953-963. Epub 2020 Oct 15.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.

Sunset yellow (SY), an azo dye, is commonly used in the food industry. The scientific literature contains little information regarding the effects of SY on small intestinal epithelial cells (IECs). In this study, a small intestinal organoid model was used in in vitro toxicological studies of SY, and intestinal inflammatory responses in vivo to SY were investigated with the dextran sulfate sodium (DSS)-induced intestinal inflammation model in C57BL/6 mice. The intestinal organoids were cultured with 2 μg/ml SY for two generations, the growth rates were analyzed, and the expressions of cell lineages were assayed. For inflammatory responses, mice were fed with a diet containing 40 mg/kg diet SY and treated with 2.5% DSS for 7 days. The results showed that SY inhibited the growth of the organoids by inhibiting the proliferation and disturbing the differentiation of IECs. Furthermore, endoplasmic reticulum (ER) stress and oxidative stress levels were elevated in SY-treated organoids. In DSS-treated mice, the disease activity index and expression levels of interleukin-1β and tumor necrosis factor-α were enhanced in the SY group, concluding that SY exacerbated DSS-induced intestinal inflammation. Taken together, these findings revealed that SY could disturb the homeostasis of the small intestinal epithelium by generating high levels of ER stress and oxidative stress, with long-term continuous consumption of SY potentially increasing the risk of intestinal inflammation.
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http://dx.doi.org/10.1002/jat.4080DOI Listing
June 2021

Milk phospholipid supplementation mediates colonization resistance of mice against Salmonella infection in association with modification of gut microbiota.

Food Funct 2020 Jul;11(7):6078-6090

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.

Gut microbiota-mediated colonization resistance against enteropathogens is known to be greatly influenced by bioactive food compounds. This work aims to investigate the effects of milk phospholipid (MP) supplementation on the colonization resistance of mice to Salmonella enterica serovar Typhimurium (S. Typhimurium) infection, with the focus mainly on the change of gut microbiota. Comparative microbiota analysis based on 16S rRNA gene sequence data of mice under different MP supplementation situations allowed us to identify specific microbiota characteristics associated with the varying degree of susceptibility to S. Typhimurium infection. We found that a moderate dietary intake of MPs (0.05 wt%) significantly increased the relative abundance of Bacteroides spp. (p < 0.05) and the propionate level (p < 0.05) in the mouse colon and enhanced colonization resistance against S. Typhimurium infection, when compared with the un-supplemented S. Typhimurium-infected mice, whereas excessive MP supplementation (0.25 wt%) did not significantly change the level of Bacteroides spp. (p > 0.05) and propionate (p > 0.05) and even enhanced the susceptibility and severity of S. Typhimurium infection. Furthermore, the inhibitory effects of Bacteroides spp. and propionate on S. Typhimurium intestinal colonization were verified in an ex vivo S. Typhimurium-infected 3D colonoid culture system. Our results showed that the supplementation of nutraceuticals may not always be the more the better, particularly under specific pathological conditions, and identification of specific gut microbiota characteristics may have the potential to become an indicator of appropriate supplementation in specific cases.
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http://dx.doi.org/10.1039/d0fo00883dDOI Listing
July 2020

Attenuated TOR signaling lengthens circadian period in .

Plant Signal Behav 2020 7;15(2):1710935. Epub 2020 Jan 7.

Key laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, People's Republic of China.

By timing many diel rhythmic events, circadian clock provides an adaptive advantage for higher plants. Meanwhile, circadian clock displays plasticity and can be entrained by the external environmental cues and internal factors. However, whether cellular energy status can regulate circadian clock is largely unknown in higher plants. The evolutionarily conserved TOR (target of rapamycin) signaling among eukaryotic organisms has been implicated as an integrator for cellular nutrient and energy status. Here, we demonstrated that chemically blocking electron transport chain of mitochondrial can lengthen the circadian period. Similarly, chemical inhibition of TOR activity by Torin 1, a specific inhibitor for TOR kinase, and knockdown of transcript levels significantly elongate the circadian period as well. Our findings imply that TOR signaling may mediate energy status-regulated circadian clock in plants, and the reciprocal regulation between the circadian clock and TOR signaling might be an evolutionary mechanism for fitness and adaptation in plants.
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http://dx.doi.org/10.1080/15592324.2019.1710935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053880PMC
January 2021

Milk phospholipids ameliorate mouse colitis associated with colonic goblet cell depletion via the Notch pathway.

Food Funct 2019 Aug 10;10(8):4608-4619. Epub 2019 Jul 10.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.

As natural emulsifiers used in food products, phospholipids have been gaining increasing attention, whereas their intestinal health impacts are still unclear. This work aims to investigate the effect of milk phospholipids (MP) on dextran sodium sulfate (DSS)-induced colitis of C57BL/6 mice, compared with soybean phospholipids (SP), as well as the related mechanism. We found that unlike SP, MP supplementation (25 mg per kg BW) had a unique beneficial effect on the 2.5% DSS induced colitis (p < 0.05) and ameliorated the injury status of goblet cells, indicated by the increased number (p < 0.05) and size (p < 0.05) of the goblet cells and more acid mucins and antimicrobial peptides (p < 0.05). Meanwhile, MP supplementation restored the over-activated Notch pathway through balancing the level of Notch pathway ligand Dll4 in the colon subepithelial layer (p < 0.05), thus leading to the increase in the Math1 expression (p < 0.05), and consequently enhanced goblet cell restitution (p < 0.05). Furthermore, MP induced more goblet cells in the colonoid and colonic myofibroblast co-culture system (p < 0.05), which highlighted the indispensable role of colonic myofibroblasts, as an intestinal stem cell niche factor, in the goblet cell modulation effect of MP. These findings indicated that phospholipids from milk instead of soybean attenuated the severity of DSS-induced mouse colitis and prevented the depletion of colonic goblet cells through balancing the over-activated Notch pathway mediated by colonic myofibroblasts.
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http://dx.doi.org/10.1039/c9fo00690gDOI Listing
August 2019

A new sensory sweetness definition and sweetness conversion method of five natural sugars, based on the Weber-Fechner Law.

Food Chem 2019 May 19;281:78-84. Epub 2018 Dec 19.

School of Food Science and Biotechnology, Zhejiang GongShang University, Zhejiang 310018, China.

This study's aim is to establish a new sensory sweetness definition and conversion method for five sugars. A "closed-type" question based on triangle test and paired comparison was used for sensory evaluation. The absolute threshold and nine sensory difference threshold values were determined and used to generate a sweet sensory difference strength curve. Defining absolute threshold of sucrose sweetness as 1, the sucrose sweetness at any concentration could be calculated via the curve. After taking the logarithm of each curve, sweetness index was calculated as 1, 1.12, 0.94, 1.29, and 1.25 for sucrose, glucose, fructose, lactose and maltose, respectively. Based on this, each sugar concentration and sweetness could be converted and calculated. Single sugar and mixed-sugars sweetness comparison experiments verified the new sweetness index and sweetness values were more accurate (83.3-100%) than those reported in previous studies. Therefore, this new definition and conversion method established more reliable references for sweet taste sensory applications.
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http://dx.doi.org/10.1016/j.foodchem.2018.12.049DOI Listing
May 2019

A Broad-Spectrum Sweet Taste Sensor Based on Ni(OH)₂/Ni Electrode.

Sensors (Basel) 2018 Aug 22;18(9). Epub 2018 Aug 22.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Zhejiang 310018, China.

A broad-spectrum sweet taste sensor based on Ni(OH)₂/Ni electrode was fabricated by the cyclic voltammetry technique. This sensor can be directly used to detect natural sweet substances in 0.1 M NaOH solution by chronoamperometry method. The current value measured by the sensor shows a linear relationship with the concentration of glucose, sucrose, fructose, maltose, lactose, xylitol, sorbitol, and erythritol (R² = 0.998, 0.983, 0.999, 0.989, 0.985, 0.990, 0.991, and 0.985, respectively). Moreover, the characteristic value of this sensor is well correlated with the concentration and relative sweetness of eight sweet substances. The good correlation between the characteristic value of six fruit samples measured by the sensor and human sensory sweetness measured by sensory evaluation (correlation coefficient = 0.95) indicates that it can reflect the sweetness of fruits containing several sweet substances. In addition, the sensor also exhibits good long-term stability over 40 days (signal ratio fluctuation ranges from 91.5% to 116.2%). Thus, this broad-spectrum sensor is promising for sweet taste sensory application.
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http://dx.doi.org/10.3390/s18092758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164501PMC
August 2018

Gli3 is a negative regulator of Tas1r3-expressing taste cells.

PLoS Genet 2018 02 7;14(2):e1007058. Epub 2018 Feb 7.

Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America.

Mouse taste receptor cells survive from 3-24 days, necessitating their regeneration throughout adulthood. In anterior tongue, sonic hedgehog (SHH), released by a subpopulation of basal taste cells, regulates transcription factors Gli2 and Gli3 in stem cells to control taste cell regeneration. Using single-cell RNA-Seq we found that Gli3 is highly expressed in Tas1r3-expressing taste receptor cells and Lgr5+ taste stem cells in posterior tongue. By PCR and immunohistochemistry we found that Gli3 was expressed in taste buds in all taste fields. Conditional knockout mice lacking Gli3 in the posterior tongue (Gli3CKO) had larger taste buds containing more taste cells than did control wild-type (Gli3WT) mice. In comparison to wild-type mice, Gli3CKO mice had more Lgr5+ and Tas1r3+ cells, but fewer type III cells. Similar changes were observed ex vivo in Gli3CKO taste organoids cultured from Lgr5+ taste stem cells. Further, the expression of several taste marker and Gli3 target genes was altered in Gli3CKO mice and/or organoids. Mirroring these changes, Gli3CKO mice had increased lick responses to sweet and umami stimuli, decreased lick responses to bitter and sour taste stimuli, and increased glossopharyngeal taste nerve responses to sweet and bitter compounds. Our results indicate that Gli3 is a suppressor of stem cell proliferation that affects the number and function of mature taste cells, especially Tas1r3+ cells, in adult posterior tongue. Our findings shed light on the role of the Shh pathway in adult taste cell regeneration and may help devise strategies for treating taste distortions from chemotherapy and aging.
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http://dx.doi.org/10.1371/journal.pgen.1007058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819828PMC
February 2018

Whole transcriptome profiling of taste bud cells.

Sci Rep 2017 08 8;7(1):7595. Epub 2017 Aug 8.

Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA, 19104, USA.

Analysis of single-cell RNA-Seq data can provide insights into the specific functions of individual cell types that compose complex tissues. Here, we examined gene expression in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiologically identified type III cells. Our RNA-Seq libraries met high quality control standards and accurately captured differential expression of marker genes for type II (e.g. the Tas1r genes, Plcb2, Trpm5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells. Bioinformatics analysis showed that genes regulating responses to stimuli were up-regulated in type II cells, while pathways related to neuronal function were up-regulated in type III cells. We also identified highly expressed genes and pathways associated with chemotaxis and axon guidance, providing new insights into the mechanisms underlying integration of new taste cells into the taste bud. We validated our results by immunohistochemically confirming expression of selected genes encoding synaptic (Cplx2 and Pclo) and semaphorin signalling pathway (Crmp2, PlexinB1, Fes and Sema4a) components. The approach described here could provide a comprehensive map of gene expression for all taste cell subpopulations and will be particularly relevant for cell types in taste buds and other tissues that can be identified only by physiological methods.
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http://dx.doi.org/10.1038/s41598-017-07746-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548921PMC
August 2017

8-Anilino-1-naphthalenesulfonate/Layered Double Hydroxide Ultrathin Films: Small Anion Assembly and Its Potential Application as a Fluorescent Biosensor.

Langmuir 2016 09 24;32(35):9015-22. Epub 2016 Aug 24.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, People's Republic of China.

The fluorescent dye 8-anilino-1-naphthalenesulfonate (ANS) is a widely used fluorescent probe molecule for biochemistry analysis. This paper reported the fabrication of ANS/layered double hydroxide nanosheets (ANS/LDH)n ultrathin films (UTFs) via the layer-by-layer small anion assembly technique based on electrostatic interaction and two possible weak interactions: hydrogen-bond and induced electrostatic interactions between ANS and positive-charged LDH nanosheets. The obtained UTFs show a long-range-ordered periodic layered stacking structure and weak fluorescence in dry air or water, but it split into three narrow strong peaks in a weak polarity environment induced by the two-dimensional (2D) confinement effect of the LDH laminate; the fluorescence intensity increases with decreasing the solvent polarity, concomitant with the blue shift of the emission peaks, which show good sensoring reversibility. Meanwhile, the UTFs exhibit selective fluorescence enhancement to the bovine serum albumin (BSA)-like protein biomolecules, and the rate of fluorescence enhancement with the protein concentration is significantly different with the different protein aggregate states. The (ANS/LDH)n UTF has the potential to be a novel type of biological flourescence sensor material.
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http://dx.doi.org/10.1021/acs.langmuir.6b01980DOI Listing
September 2016

An Inexpensive Co-Intercalated Layered Double Hydroxide Composite with Electron Donor-Acceptor Character for Photoelectrochemical Water Splitting.

Sci Rep 2015 Jul 15;5:12170. Epub 2015 Jul 15.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, P. Box 98, 100029, Beijing (P. R. China).

In this paper, the inexpensive 4,4-diaminostilbene-2,2-disulfonate (DAS) and 4,4-dinitro-stilbene-2,2- disulfonate (DNS) anions with arbitrary molar ratios were successfully co-intercalated into Zn2Al-layered double hydroxides (LDHs). The DAS(50%)-DNS/LDHs composite exhibited the broad UV-visible light absorption and fluorescence quenching, which was a direct indication of photo-induced electron transfer (PET) process between the intercalated DAS (donor) and DNS (acceptor) anions. This was confirmed by the matched HOMO/LUMO energy levels alignment of the intercalated DAS and DNS anions, which was also compatible for water splitting. The DAS(50%)-DNS/LDHs composite was fabricated as the photoanode and Pt as the cathode. Under the UV-visible light illumination, the enhanced photo-generated current (4.67 mA/cm(2) at 0.8 V vs. SCE) was generated in the external circuit, and the photoelectrochemical water split was realized. Furthermore, this photoelectrochemical water splitting performance had excellent crystalline, electrochemical and optical stability. Therefore, this novel inorganic/organic hybrid photoanode exhibited potential application prospect in photoelectrochemical water splitting.
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http://dx.doi.org/10.1038/srep12170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502407PMC
July 2015

Orderly ultrathin films based on perylene/poly(N-vinyl carbazole) assembled with layered double hydroxide nanosheets: 2D fluorescence resonance energy transfer and reversible fluorescence response for volatile organic compounds.

Adv Mater 2012 Nov 31;24(45):6053-7. Epub 2012 Aug 31.

State Key Laboratory of Chemical, Resource Engineering, Beijing University of Chemical Technology, Beijing, P R China.

Neutral poly(N-vinyl carbazole) (PVK) and perylene are coassembled within the interlayers of layered double hydroxide (LDH) nanosheets to form ([email protected]/LDH)(n) ultrathin films by the hydrogen-bond layer-by-layer assembly method. An efficient 2D fluorescence resonance energy transfer (FRET) process from PVK to perylene is demonstrated, and this FRET process can be inhibited/recovered reversibly by the adsorption/desorption of common volatile organic compounds (VOCs).
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http://dx.doi.org/10.1002/adma.201203040DOI Listing
November 2012
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