Publications by authors named "Yumei Liu"

240 Publications

Proteomic analysis of hexahydro-β-acids/hydroxypropyl-β-cyclodextrin inhibit Listeria monocytogenes.

Appl Microbiol Biotechnol 2022 Jan 11;106(2):755-771. Epub 2022 Jan 11.

School of Chemical Engineering and Technology, Xinjiang University, Urumqi, 830046, China.

Food safety affected by food-borne pathogen has received increasing attention by researchers. Listeria monocytogenes (L. monocytogenes), widespread in the environment, could easily cause some diseases. The aim of this study was to investigate how L. monocytogenes ATCC 19,115 regulated and shaped its proteome in response to hexahydro-β-acids (HBA) formed inclusion complex with hydroxypropyl-β-cyclodextrin (HP-β-CD), compared to untreated cells growing under optimal conditions. HP-β-CD enhanced the solubility of HBA to 0.589 g/100 mL. The minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of HBA/HP-β-CD to L. monocytogenes were 25 μg/mL and 100 μg/mL, respectively. Scanning electron microscope (SEM) images demonstrated that HBA could destroy the cell membrane of L. monocytogenes. The proteomic analysis revealed that 2882 proteins were initially identified, among which 153 and 201 proteins were differentially upregulated and downregulated respectively. Changes of L. monocytogenes proteome in response to treatments were mainly related to carbohydrate metabolism, protein synthesis, ribosome composition proteins, cell wall composition proteins, and cell division anomalies process. This research is conducive to understanding the molecular mechanisms underlying the inhibiting effects of HBA/HP-β-CD against L. monocytogenes, providing novel insights for further development of HBA/HP-β-CD antimicrobial agents. KEY POINTS: • MIC and MBC of HBA/HP-β-CD to L. monocytogenes were 25 μg/mL and 100 μg/mL. • HBA/HP-β-CD cause significant changes in bacterial proteome. • The process of ribosome composition and carbohydrate metabolism was inhibited.
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http://dx.doi.org/10.1007/s00253-022-11764-xDOI Listing
January 2022

Role of Cytokines on the Progression of Liver Fibrosis in Mice Infected with .

Infect Drug Resist 2021 24;14:5651-5660. Epub 2021 Dec 24.

State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang, 830011, People's Republic of China.

Background: Liver fibrosis is a significant pathological change of () infection. This study aimed to explore the role of cytokines on the progression of liver fibrosis in mice infected with .

Methods: Liver histopathological features at 2, 8, 30, 90 and 180 d were quantified by inflammatory severity score. The expression levels of inflammatory cytokines, fibrosis-related cytokines and hepatic cell apoptosis were measured using qRT-PCR and immunohistochemistry.

Results: At the early stage of infection, parasite stimulation triggers the rapid recruitment of immune cells, such as macrophages and neutrophils. These infiltrated immune cells then produce a large number of cytokines, such as iNOS (inducible nitric oxide synthase), a pro-inflammatory cytokine; TGF-β (transforming growth factor) activated HSCs (hepatic stellate cells) to promote the proliferation of fibroblasts and secretion of ECM (extracellular matrix); MMP9 (matrix metalloproteinase 9) degraded basal ECM and facilitated its replacement by a highly dense interstitial matrix. At the middle and late stages of infection, the expression of IL-10 (interleukin-10) with general inhibitory effect was increased. The imbalance of fiber formation and degradation aggravated liver fibrosis. Meanwhile, the whole process of infection was accompanied by the necrosis and apoptosis of hepatic cells.

Conclusion: Along with the expansion of parasitic infection, dynamic changes in cytokine expression were observed on the liver fibrosis progression, which is helpful to provide some new ideas for the prevention and treatment of liver fibrosis in mice infected with .
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http://dx.doi.org/10.2147/IDR.S344508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714463PMC
December 2021

Cyclodextrin-Containing Hydrogels: A Review of Preparation Method, Drug Delivery, and Degradation Behavior.

Int J Mol Sci 2021 Dec 16;22(24). Epub 2021 Dec 16.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China.

Hydrogels possess porous structures, which are widely applied in the field of materials and biomedicine. As a natural oligosaccharide, cyclodextrin (CD) has shown remarkable application prospects in the synthesis and utilization of hydrogels. CD can be incorporated into hydrogels to form chemically or physically cross-linked networks. Furthermore, the unique cavity structure of CD makes it an ideal vehicle for the delivery of active ingredients into target tissues. This review describes useful methods to prepare CD-containing hydrogels. In addition, the potential biomedical applications of CD-containing hydrogels are reviewed. The release and degradation process of CD-containing hydrogels under different conditions are discussed. Finally, the current challenges and future research directions on CD-containing hydrogels are presented.
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http://dx.doi.org/10.3390/ijms222413516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703588PMC
December 2021

Comparative Proteome Analysis Indicates The Divergence between The Head and Tail Regeneration in Planarian.

Cell J 2021 Nov 23;23(6):640-649. Epub 2021 Nov 23.

Animal Science and Technology School, Henan University of Science and Technology, Luoyang, China.

Objective: Even a small fragment from the body of planarian can regenerate an entire animal, implying that the different fragments from this flatworm eventually reach the same solution. In this study, our aim was to reveal the differences and similarities in mechanisms between different regenerating fragments from this worm.

Materials And Methods: In this experimental study, we profiled the dynamic proteome of regenerating head and tail to reveal the differences and similarities between different regenerating fragments using 2-DE combined with MALDITOF/ TOF MS.

Results: Proteomic profiles of head and tail regeneration identified a total of 516 differential expressed proteins (DEPs) and showed a great difference in quantity and fold changes of proteome profiles between the two scenarios. Briefly, out of the 516 DEPs, 314 were identified to be specific for anterior regeneration, while 165 were specific for posterior regeneration. Bioinformatics analysis showed a wide discrepancy in biological activities between two regenerative processes; especially, differentiation and development and signal transduction in head regeneration were much more complex than that in tail regeneration. Protein functional analysis combined with protein-protein interaction (PPI) analysis showed a significant contribution of both Wnt and BMP signaling pathways to head regeneration not but tail regeneration. Additionally, several novel proteins showed completely opposite expression between head and tail regeneration.

Conclusion: Proteomic profiles of head and tail regeneration identified a total of 516 differential expressed proteins (DEPs) and showed a great difference in quantity and fold changes of proteome profiles between the two scenarios. Briefly, out of the 516 DEPs, 314 were identified to be specific for anterior regeneration, while 165 were specific for posterior regeneration. Bioinformatics analysis showed a wide discrepancy in biological activities between two regenerative processes; especially, differentiation and development and signal transduction in head regeneration were much more complex than that in tail regeneration. Protein functional analysis combined with protein-protein interaction (PPI) analysis showed a significant contribution of both Wnt and BMP signaling pathways to head regeneration not but tail regeneration. Additionally, several novel proteins showed completely opposite expression between head and tail regeneration.
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http://dx.doi.org/10.22074/cellj.2021.7689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665983PMC
November 2021

Lung ultrasound: a valuable tool for assessing COVID-19 patients with different severity.

Chin Med J (Engl) 2021 Dec 22. Epub 2021 Dec 22.

Department of Critical Care Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, Guangdong, China Department of Healthcare-Associated Infection Management, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China Department of Respiratory Medicine, Hankou Hospital of Wuhan, Wuhan, Hubei, China.

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http://dx.doi.org/10.1097/CM9.0000000000001921DOI Listing
December 2021

Concurrent H Generation and Formate Production Assisted by CO Absorption in One Electrolyzer.

Small Methods 2021 Nov 7;5(11):e2100871. Epub 2021 Oct 7.

School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.

Electrolyzers coupling electrocatalytic hydrogen evolution with oxidation reactions of small organic molecules have the merits of reducing cell voltage and generating high-value products. Herein, an electrolyzer is designed and optimized that can simultaneously achieve efficient hydrogen generation at the cathode, CO absorption by the catholyte, and methanol upgrading to formate at the anode. For these purposes, transition metal phosphides are used as the low-cost catalysts. The unique electrolyzer exhibits a low working voltage of 1.1 V at 10 mA cm . Under optimal conditions, the Faraday efficiencies of hydrogen evolution and formic acid conversion reactions, which are the reaction products at the cathode and anode, respectively, are nearly 100% at various current densities from 10 to 400 mA cm . Meanwhile, the CO absorption rate is about twice that of the hydrogen generation rate, which is close to the theoretical value. An innovative and energy-efficient strategy is presented in this work to realize simultaneous hydrogen production and CO capture based on low-cost catalyst materials.
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http://dx.doi.org/10.1002/smtd.202100871DOI Listing
November 2021

SARS-CoV-2 vaccine-induced immune thrombotic thrombocytopenia.

Thromb Res 2022 01 6;209:75-79. Epub 2021 Dec 6.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address:

SARS-CoV-2 vaccines have been carefully developed and significantly alleviate the global pandemic. However, a rare but severe complication after vaccination of adenoviral vector vaccines has attracted worldwide attention. It is characterized by thrombosis at unusual sites (often cerebral or abdominal), thrombocytopenia, and the presence of antibodies against platelet factor 4 (PF4), termed vaccine-induced immune thrombotic thrombocytopenia (VITT). Its pathogenesis is similar to that of heparin-induced thrombocytopenia (HIT). VITT progresses rapidly and has a high mortality rate. Clinicians and the public should raise their vigilance to this disease so that accurate and timely treatment is provided.
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http://dx.doi.org/10.1016/j.thromres.2021.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8647389PMC
January 2022

Genetic mapping and candidate gene identification of BoGL5, a gene essential for cuticular wax biosynthesis in broccoli.

BMC Genomics 2021 Nov 10;22(1):811. Epub 2021 Nov 10.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.

Background: The aerial organs of most terrestrial plants are covered by cuticular waxes, which impart plants a glaucous appearance and play important roles in protecting against various biotic and abiotic stresses. Despite many glossy green (wax-defective) mutants being well characterized in model plants, little is known about the genetic basis of glossy green mutant in broccoli.

Results: B156 is a spontaneous broccoli mutant showing a glossy green phenotype. Detection by scanning electron microscopy (SEM) and chromatography-mass spectrometry (GC-MS) revealed that B156 is a cuticular wax-defective mutant, lacking waxes mostly longer than C28. Inheritance analysis revealed that this trait was controlled by a single recessive gene, BoGL5. Whole-genome InDel markers were developed, and a segregating F population was constructed to map BoGL5. Ultimately, BoGL5 was mapped to a 94.1 kb interval on C01. The BoCER2 gene, which is homologous to the Arabidopsis CER2 gene, was identified as a candidate of BoGL5 from the target interval. Sequence analyses revealed that Bocer2 in B156 harbored a G-to-T SNP mutation at the 485th nucleotide of the CDS, resulting in a W-to-L transition at the 162nd amino acid, a conserved site adjacent to an HXXXD motif of the deduced protein sequence. Expression analysis revealed that BoCER2 was significantly down-regulated in the leaves, stems, and siliques of B156 mutant than that of B3. Last, ectopic expression of BoCER2 in A. thaliana could, whereas Bocer2 could not, rescue the phenotype of cer2 mutant.

Conclusions: Overall, this study mapped the locus determining glossy phenotype of B156 and proved BoCER2 is functional gene involved in cuticular wax biosynthesis which would promotes the utilization of BoCER2 to enhance plant resistance to biotic and abiotic stresses, and breeding of B. oleracea cultivars with glossy traits.
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http://dx.doi.org/10.1186/s12864-021-08143-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582161PMC
November 2021

[Protective effect of oleanolic acid on L02 hepatocyte injury induced by HgCl_2].

Wei Sheng Yan Jiu 2021 Sep;50(5):781-787

School of Public Health, Weifang Medical College, Weifang 261053, China Weifang Key Laboratory of Health Inspection and Quarantine, Weifang 261053, China.

Objective: To investigate the protective effect of oleanolic acid(OA) on HgCl_2 induced liver injury.

Methods: L02 cells were divided into four groups according to different treatment, control group(Con), oleanolic acid group(OA, 10 μmol/L), HgCl_2 group(HgCl_2, 40 μmol/L) and oleanolic acid + HgCl_2 group(OA + HgCl_2). Cells of control group were given serum-free medium, cells of OA group were pretreated with OA solution for 8 hours, cells of HgCl_2 group were exposed to HgCl_2 solution for 6 hours, cells of OA + HgCl_2 group were pretreated with OA solution for 8 hours, and then exposed to HgCl_2 solution for 6 hours. MTT assay was used to detect cell viability. Laser confocal scanning was used to detect JC-1 probe fluorescence intensity to determine mitochondrial membrane potential. DCFH-DA fluorescence probe combined with flow cytometry was used to detect reactive oxygen species(ROS) level. Annexin V/PI double staining method combined with flow cytometry was used to determine cell apoptosis rate. Catalase(CAT), total superoxide dismutase(T-SOD), glutathione(GSH), malondialdehyde(MDA), Caspase 3 and Caspase 9 kits combined with enzyme labeled instrument were used to determine their activity or content respectively.

Results: Compared with the control group, 40 μmol/L HgCl_2 could significantly reduce cell viability, the level was 0.52±0.03(P<0.05), OA pretreatment could significantly inhibit the decrease of cell viability induced by HgCl_2, the level was 0.86±0.05(P<0.05). The result of mitochondrial membrane potential detection showed that cell exposed to 40 μmol/L HgCl_2 significantly reduced the intensity of red fluorescence, and the ratio of red to green fluorescence was 0.23±0.02(P<0.05). OA pretreatment significantly increased red fluorescence, and the ratio of red fluorescence to green fluorescence was 1.32±0.08, which was significantly higher than that of HgCl_2(P<0.05). After exposure to 40 μmol/L HgCl_2, the relative fluorescence intensity of ROS was 1.21±0.07, the apoptosis rate was about 8%, the activity levels of Casepase 3 and Casepase 9 were 3.11±0.20 and 2.94±0.17, respectively, which were all significantly higher than those in the control group(P<0.05). OA pretreatment could significantly alleviate the changes of the above indexes, and the difference was statistically significant compared with HgCl_2 group(P<0.05). The level of T-SOD in HgCl_2 group was(7.68±0.39)U/mL, which was significantly lower than that in control group(P<0.05). Compared to the control group, the level of MDA was significantly increased to(4.99±0.26)nmol/mg(P<0.05). OA pretreatment significantly increased level of T-SOD and decreased the level of MDA, the levels were(13.97±0.71)U/mL and(3.01±0.17)nmol/mg, respectively(P<0.05).

Conclusion: A certain concentration of HgCl_2 can induce hepatocyte damage. OA pretreatment may reduce cell damage by improving oxidative stress.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.05.013DOI Listing
September 2021

Ten-eleven translocation proteins (TETs): tumor suppressors or tumor enhancers?

Front Biosci (Landmark Ed) 2021 10;26(10):895-915

Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.

The epigenetic memory stored in the dynamic modifications, such as base modifications of cytosine (C) in DNA, including methylation/hydroxymethylation/demethylation, causes heritable phenotypes via regulating gene expression without alteration of DNA sequence. The process from cytosine modification to the epigenetic effect is orchestrated by complicated machinery consisting of writers, erasers, readers, and other factors. The two major forms of cytosine modification include methylcytosine (5-mC) and hydroxymethylcytosine (5-hmC). DNA methyltransferases (DNMTs) including DNMT1, DNMT3A, and DNMT3B function as writers for 5-mC. The ten-eleven translocation proteins (TET) including TET1, TET2, and TET3 in the mammalian genome are responsible for hydroxymethylation of 5-mC to generate 5-hmC, 5-formylcytosine (5-fC), and 5-carboxylcytosine (5-caC). The 5-mC and 5-hmC have become the two most extensively investigated epigenetic markers, and the dynamic balance of these two markers shape the landscape of the epigenome, functioning as a platform to regulate gene expression epigenetically. The landscape of the 5-hmC in epigenome is precisely and tightly regulated during the development. Aberrant alterations of the epigenetic regulation may cause severe consequences such as phenotype change as well as initiation of disease. Progressively, significant achievements have been made in characterization of writers, erasers, and readers of 5-mC and 5-hmC, as well as the contribution of aberrant alteration of 5-hmC/5-mC landscape to the pathogenesis of human diseases, such as cancers and neurological disorders. This article will highlight the research advances in the distinct contribution of TET proteins as suppressors or promoters to the pathogenesis of tumorigenesis and progression. Furthermore, this article also discusses the challenges and the directions for research in the future.
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http://dx.doi.org/10.52586/4996DOI Listing
October 2021

Dogs lacking Apolipoprotein E show advanced atherosclerosis leading to apparent clinical complications.

Sci China Life Sci 2021 Oct 21. Epub 2021 Oct 21.

Key Laboratory of Molecular Developmental Biology, and CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Atherosclerotic cardiovascular disease resulting from dysregulated lipid metabolism is the leading cause of morbidity and mortality worldwide. Apolipoprotein E (ApoE) plays a critical role in cholesterol metabolism. Knockouts in lipid-metabolizing proteins including ApoE in multiple model organisms such as mice and rats exhibiting elevated levels of cholesterol have been widely used for dissecting the pathology of atherosclerosis, but few of these animal models exhibit advanced atherosclerotic plaques leading to ischemia-induced clinical symptoms, limiting their use for translational studies. Here we report hypercholesterolemia and severe atherosclerosis characterized by stenosis and occlusion of arteries, together with clinical manifestations of stroke and gangrene, in ApoE knockout dogs generated by CRISPR/Cas9 and cloned by somatic cell nuclear transfer technologies. Importantly, the hypercholesterolemia and atherosclerotic complications in F0 mutants are recapitulated in their offspring. As the ApoE-associated atherosclerosis and clinical manifestations in mutant dogs are more similar to that in human patients compared with those in other animal models, these mutant dogs will be invaluable in developing and evaluating new therapies, including endovascular procedures, against atherosclerosis and related disorders.
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http://dx.doi.org/10.1007/s11427-021-2006-yDOI Listing
October 2021

Genome-wide characterization and analysis of the anthocyanin biosynthetic genes in Brassica oleracea.

Planta 2021 Oct 11;254(5):92. Epub 2021 Oct 11.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.

Main Conclusion: From Brassica oleracea genome, 88 anthocyanin biosynthetic genes were identified. They expanded via whole-genome or tandem duplication and showed significant expression differentiation. Functional characterization revealed BoMYB113.1 as positive and BoMYBL2.1 as negative regulators responsible for anthocyanin accumulation. Brassica oleracea produces various health-promoting phytochemicals, including glucosinolates, carotenoids, and vitamins. Despite the anthocyanin biosynthetic pathways in the model plant Arabidopsis thaliana being well characterized, little is known about the genetic basis of anthocyanin biosynthesis in B. oleracea. In this study, we identified 88 B. oleracea anthocyanin biosynthetic genes (BoABGs) representing homologs of 46 Arabidopsis anthocyanin biosynthetic genes (AtABGs). Most anthocyanin biosynthetic genes, having expanded via whole-genome duplication and tandem duplication, retained more than one copy in B. oleracea. Expression analysis revealed diverse expression patterns of BoABGs in different tissues, and BoABG duplications showed significant expression differentiation. Additional expression analysis and functional characterization revealed that the positive regulator BoMYB113.1 and negative regulator BoMYBL2.1 may be key genes responsible for anthocyanin accumulation in red cabbage and ornamental kale by upregulating the expression of structural genes. This study paves the way for a better understanding of anthocyanin biosynthetic genes in B. oleracea and should promote breeding for anthocyanin content.
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http://dx.doi.org/10.1007/s00425-021-03746-6DOI Listing
October 2021

The high-risk factors of different severities of bronchopulmonary dysplasia (BPD) based on the national institute of child health and human development (NICHD) diagnosis criteria in 2018.

J Bras Pneumol 2021 29;47(5):e20210125. Epub 2021 Sep 29.

Department of Clinical Medicine, School of Medicine, South China University of Technology, Guangzhou, China.

Objective: To investigate the clinical characteristics of preterm infants with different severities of bronchopulmonary dysplasia (BPD) and disclose the high-risk factors of exacerbating BPD.

Methods: Collection of clinical data of 91 preterm infants admitted to the NICU and diagnosed with BPD, categorized in groups according to the disease severity: 41 mild cases,, 24 moderate cases, and 26 severe cases. Comparison and analysis of perinatal risk factors, treatment, complications and prognosis of the infants with different severity degrees.

Results: The severe group had a higher proportion of infants with congenital heart disease (CHD) higher than the moderate group (P < 0.05), and a higher ratio of pneumonia and mechanical ventilation (MV) ≥ seven days than the mild group (P < 0.05). The severe group also presented higher reintubation incidence than both the mild and moderate groups (P < 0.05). The groups presented different (P < 0.05) incidence rates of hemodynamically significant patent ductus arteriosus (hsPDA) . Ridit analysis suggested that the premature infants (PIs) with hsPDA, multiple microbial pulmonary infections, or Klebsiella pneumoniae pneumonia had more severe illness.

Conclusion: CHD, hsPDA, MV ≥ seven days, reintubation, pneumonia, especially multiple microbial pulmonary infections, and Klebsiella pneumoniae pneumonia are correlated with the severity of BPD and can be used as BPD progression predictor.
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http://dx.doi.org/10.36416/1806-3756/e20210125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642818PMC
October 2021

Prognostic Value of Leucocyte to High-Density Lipoprotein-Cholesterol Ratios in COVID-19 Patients and the Diabetes Subgroup.

Front Endocrinol (Lausanne) 2021 13;12:727419. Epub 2021 Sep 13.

Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Blood parameters, such as neutrophil-to-lymphocyte ratio, have been identified as reliable inflammatory markers with diagnostic and predictive value for the coronavirus disease 2019 (COVID-19). However, novel hematological parameters derived from high-density lipoprotein-cholesterol (HDL-C) have rarely been studied as indicators for the risk of poor outcomes in patients with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infection. Here, we aimed to assess the prognostic value of these novel biomarkers in COVID-19 patients and the diabetes subgroup.

Methods: We conducted a multicenter retrospective cohort study involving all hospitalized patients with COVID-19 from January to March 2020 in five hospitals in Wuhan, China. Demographics, clinical and laboratory findings, and outcomes were recorded. Neutrophil to HDL-C ratio (NHR), monocyte to HDL-C ratio (MHR), lymphocyte to HDL-C ratio (LHR), and platelet to HDL-C ratio (PHR) were investigated and compared in both the overall population and the subgroup with diabetes. The associations between blood parameters at admission with primary composite end-point events (including mechanical ventilation, admission to the intensive care unit, or death) were analyzed using Cox proportional hazards regression models. Receiver operating characteristic curves were used to compare the utility of different blood parameters.

Results: Of 440 patients with COVID-19, 67 (15.2%) were critically ill. On admission, HDL-C concentration was decreased while NHR was high in patients with critical compared with non-critical COVID-19, and were independently associated with poor outcome as continuous variables in the overall population (HR: 0.213, 95% CI 0.090-0.507; HR: 1.066, 95% CI 1.030-1.103, respectively) after adjusting for confounding factors. Additionally, when HDL-C and NHR were examined as categorical variables, the HRs and 95% CIs for tertile 3 vs. tertile 1 were 0.280 (0.128-0.612) and 4.458 (1.817-10.938), respectively. Similar results were observed in the diabetes subgroup. ROC curves showed that the NHR had good performance in predicting worse outcomes. The cutoff point of the NHR was 5.50. However, the data in our present study could not confirm the possible predictive effect of LHR, MHR, and PHR on COVID-19 severity.

Conclusion: Lower HDL-C concentrations and higher NHR at admission were observed in patients with critical COVID-19 than in those with noncritical COVID-19, and were significantly associated with a poor prognosis in COVID-19 patients as well as in the diabetes subgroup.
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http://dx.doi.org/10.3389/fendo.2021.727419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473871PMC
October 2021

A review of the mechanisms of keratinocytes damage caused by Staphylococcus aureus infection in patients with atopic dermatitis.

J Leukoc Biol 2021 12 29;110(6):1163-1169. Epub 2021 Sep 29.

Institute of Dermatology, Guangzhou Medical University, Guangzhou, China.

The dysregulation of skin microflora in patients with atopic dermatitis (AD) has become a research hotspot in recent years. Metagenomic studies have shown that microbial diversity is decreased, whereas the Staphylococcus aureus infection is increased in AD. Keratinocytes are the primary barrier against the invasion of external pathogenic microorganisms. Staphylococcus aureus infection can abnormally activate innate and adaptive immune responses in keratinocytes, resulting in a vicious cycle between Staphylococcus aureus infection and AD. This article reviews the mechanisms of inflammatory damage of keratinocytes induced by Staphylococcus aureus infection in patients with AD, providing a theoretical basis for the study of new targeted drugs. This review also suggests for the management of Staphylococcus aureus infection in patients with AD.
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http://dx.doi.org/10.1002/JLB.3MR0921-030RRRDOI Listing
December 2021

The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture.

Regen Biomater 2021 Oct 7;8(6):rbab050. Epub 2021 Sep 7.

College of Medicine, Southwest Jiaotong University, No.111 North 1st Section of Second Ring Road, Chengdu, Sichuan 610031, China.

Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds, thus enhancing the differentiation of stem cells towards the osteogenic phenotype. However, the effects of macropore size on osteogenic differentiation of stem cells under dynamic condition are still unclear. Therefore, the objective of this study was to investigate the effects of macropore size of hydroxyapatite (HAp) scaffolds on osteogenic differentiation of bone mesenchymal stem cells under static and perfusion culture conditions. cell culture results showed that cell proliferation, alkaline phosphate (ALP) activity, mRNA expression of ALP, collagen-I (Col-I), osteocalcin (OCN) and osteopontin (OPN) were enhanced when cultured under perfusion condition in comparison to static culture. Under perfusion culture condition, the ALP activity and the gene expression of ALP, Col-I, OCN and OPN were enhanced with the macropore size decreasing from 1300 to 800 µm. However, with the further decrease in macropore size from 800 to 500 µm, the osteogenic related gene expression and protein secretion were reduced. Computational fluid dynamics analysis showed that the distribution areas of medium- and high-speed flow increased with the decrease in macropore size, accompanied by the increase of the fluid shear stress within the scaffolds. These results confirm the effects of macropore size on fluid flow stimuli and cell differentiation, and also help optimize the macropore size of HAp scaffolds for bone tissue engineering.
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http://dx.doi.org/10.1093/rb/rbab050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457200PMC
October 2021

Formation chitosan-based hydrogel film containing silicon for hops β-acids release as potential food packaging material.

Int J Biol Macromol 2021 Nov 21;191:288-298. Epub 2021 Sep 21.

School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830046, China. Electronic address:

Hydrogel film composed of chitosan (CS) as raw material was prepared by free radical polymerization. Silicon was introduced into the hydrogel film in different ways (covalent/non-covalent) to improve the physical properties of the film, and β-acids were loaded to enhance the antibacterial activity of the film. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analysis were used to characterize the structure of films. The mechanical results indicated that when nano-silica (0.3%) was introduced into film (containing 0.2% β-acids) by non-covalently bond, the tensile strength increased to 8.59 MPa. Meanwhile silicon (0.3%) entered the film by covalent bonding, the tensile strength increased to 7.99 MPa. The films loaded with β-acids had well ability to blocks ultraviolet rays and exhibited inhibitory effect on E. coli and S. aureus. In the PBS (37 °C, pH = 7.4) simulant solution, the release mechanism of most films to release the β-acids followed non-Fick diffusion (n > 0.5). It could be concluded that the prepared hydrogel films loading with β-acids had broad application prospects in food packaging material with antibacterial property and controlled release.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.086DOI Listing
November 2021

Physical growth and brain MRI predict the neurodevelopmental outcomes in very low birth weight infants at 2-year-old.

Neuro Endocrinol Lett 2021 Sep 10;42(5):321-330. Epub 2021 Sep 10.

Department of NICU, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.

Objective: The purpose of the current study was to determine the predictive effect of physical growth and brain magnetic resonance imaging (MRI) on neurodevelopmental outcomes in very low birth weight infants (VLBW) infants.

Materials And Methods: A total of 85 VLBW infants were included in the current study. They were cared according to the guideline of preterm management during hospitalization, and to planned follow-up rules after discharged strictly. All patients enrolled in the present study had undergone measurement of weight, length and head circumference and reported on the infants' weight-for-age z-score (WAZ), height-for-age Z-score (HAZ), head circumference-for-age Z-score (HCZ), and weight-for-height Z score (WHZ).

Results: At 29.38 ± 1.70 weeks old, the birth weight was 1240.06 ± 249.46g. MDI decreased gradually with the increase of corrective age (p<0.001), and MDI at 18 months of age decreased significantly compared to normal infants and young children of the same age (p<0.05), while at 24 months of age there was no significant difference between MDI and normal peers. Correcting PDI in 3, 6, 12, 18 and 24 months was significantly lower than that of normal infants and young children of the same age (p<0.05) and did not show a trend that changed with the correction of monthly age. WHZ gradually approaches normal as the age of the month increases (p<0.05), while HCZ decreases gradually with the correction of the age of the month (p<0.05).

Conclusion: VLBW has obvious motor development disorders, and there is no difference between intellectual development and healthy young children. MDI rises early and then gradually declines, eventually becoming 2 years old similar to that of healthy young children. PDI has consistently shown a significant decrease in infants and young children of the same age, and has not shown a trend that changes with the correction of monthly age. There is a great correlation between infancy physical development and long-term neurodevelopment, MRI at 12 months old is a valuable prediction method.
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September 2021

sLOX-1: A Molecule for Evaluating the Prognosis of Recurrent Ischemic Stroke.

Neural Plast 2021 28;2021:6718184. Epub 2021 Aug 28.

Institute of Cerebrovascular Disease Research and Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing 100000, China.

Several clinical parameters and biomarkers have been proposed as prognostic markers for stroke. However, it has not been clarified whether the risk factors affecting the prognosis of patients with recurrent and first-ever stroke are similar. In this study, we aimed to explore the relationship between soluble lectin-like oxidized low-density lipoprotein receptor 1 (sLOX-1) levels and the prediction of the functional outcome in patients with recurrent and first-ever stroke. A total of 266 patients with recurrent and first-ever stroke, who underwent follow-up for 3 months, were included in this study. Plasma samples were collected within 24 h after onset. The results showed that biomarkers for the prognosis of patients with recurrent stroke were different from that of those with first-ever stroke. sLOX-1 levels were correlated with modified Rankin Scale scores of patients with recurrent stroke alone ( = 0.3232, = 0.001). sLOX-1 levels were also associated with an increased risk of unfavorable outcomes in patients with recurrent stroke with an adjusted odds ratio of 1.489 (95% confidence interval, 1.204-1.842, < 0.0001). Combining the risk factors showed greater accuracy for prognosis, yielding a sensitivity of 93.2% and a specificity of 75%, with an area under the curve of 0.916, evaluated by the receiver operating characteristic curve. These findings suggest that the diagnosis and prognosis are different between patients with recurrent stroke and those with first-ever stroke, and sLOX-1 level is an independent prognostic marker in patients with recurrent stroke.
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http://dx.doi.org/10.1155/2021/6718184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419492PMC
August 2021

Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples.

Biosensors (Basel) 2021 Aug 16;11(8). Epub 2021 Aug 16.

College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China.

Lead (Pb) pollution is a serious food safety issue, rapid detection of Pb residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb supervision in food samples. Pb specific aptamer can bolster a transition of G-quadruplex structural response to Pb; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb testing associated with food safety.
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http://dx.doi.org/10.3390/bios11080274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391220PMC
August 2021

Chitosan-silica with hops β-acids added films as prospective food packaging materials: Preparation, characterization, and properties.

Carbohydr Polym 2021 Nov 20;272:118457. Epub 2021 Jul 20.

School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830046, China. Electronic address:

In this study, silica (SiO) and β-acids were added to the chitosan films in order to improve the film's properties. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD) were used to explore the structure of film. The results of mechanical test indicated that the film containing SiO (0.3%) and β-acids (0.3%) could obtain a significant tensile strength (10.04 MPa). The complex films possessed a good inhibitory effect on three types of bacteria, and good antioxidant activity (>56%, DPPH). The release mechanism of β-acids from the films exhibited Fickian diffusion (n < 0.45). During the storage of soybean oil, the films could well control the changes of the peroxide value, acid value and thiobarbituric acid reactant content. Overall, the biofilms not only possess good physical and chemical properties, but also prolongs the time of food storage.
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http://dx.doi.org/10.1016/j.carbpol.2021.118457DOI Listing
November 2021

Study on Virulence Genes of Type III Secretion System of Pseudomonas aeruginosa in Xinjiang Province.

Clin Lab 2021 Aug;67(8)

Background: To investigate the distribution of virulence genes exoS and exoU of Pseudomonas aeruginosa type III secretion system and their antimicrobial resistance characteristics in Xinjiang Province.

Methods: A total of 228 isolates of Pseudomonas aeruginosa were collected from January 2017 to April 2017 in our hospital. The VITEK2-compac system was used for strain identification and antimicrobial susceptibility test. The disk diffusion method was used for antimicrobial susceptibility supplementation. PCR method was used for detection of exoS and exoU virulence gene.

Results: Among 228 isolates of Pseudomonas aeruginosa, 178 (78.07%) were positive for exoS gene, 91 (39.91%) were positive for exoU gene, and 21.49% of the isolates carried both genes (exoU+/exoS+). A total of 30 MDR strains were detected, accounting for 13.16%. The antimicrobial resistance of the exoU+ group was 76.67%, which was significantly higher than that of the exoU-group (23.33%). The difference was statistically significant (p < 0.001). The detection rate of fluoroquinolone-insensitive strains in exoU+ group was as high as 57.45%, which was significantly higher than 42.55% in exoU-group and the difference was statistically significant (p < 0.05). The 30-day mortality rate of the exoU+ group was 8.79%, which was higher than that of the exoU-group (4.38%), and the difference was statistically significant (p < 0.05).

Conclusions: The expression of exoU gene is associated with multidrug resistance, fluoroquinolone resistance, and prognosis. We should enhance the detection of drug resistance and study the pathogenesis and regulation mecha-nism of T3SS, in order to provide new ideas for the design of reasonable treatment strategies and the development of new therapeutic drugs.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200815DOI Listing
August 2021

rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure.

Cell Biosci 2021 Jul 21;11(1):138. Epub 2021 Jul 21.

School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, People's Republic of China.

Background: Pseudomonas aeruginosa is a notorious opportunistic pathogen causing various types of biofilm-related infections. Biofilm formation is a unique microbial strategy that allows P. aeruginosa to survive adverse conditions such as antibiotic treatment and human immune clearance.

Results: In this study, we experimentally evolved P. aeruginosa PAO1 biofilms for cyclic treatment in the presence of high dose of imipenem, and enriched hyperbiofilm mutants within six cycles in two independent lineages. The competition assay showed that the evolved hyperbiofilm mutants can outcompete the ancestral strain within biofilms but not in planktonic cultures. Whole-genome sequencing analysis revealed the hyperbiofilm phenotype is caused by point mutations in rpoS gene in all independently evolved mutants and the same mutation was found in P. aeruginosa clinical isolates. We further showed that mutation in rpoS gene increased the intracellular c-di-GMP level by turning on the expression of the diguanylate cyclases. Mutation in rpoS increased pyocyanin production and virulence in hyperbiofilm variants.

Conclusion: Here, our study revealed that antibiotic treatment of biofilm-related P. aeruginosa infections might induce a hyperbiofilm phenotype via rpoS mutation, which might partially explain antimicrobial treatment failure of many P. aeruginosa biofilm-related infections.
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http://dx.doi.org/10.1186/s13578-021-00655-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293535PMC
July 2021

Engineering a probiotic strain of Escherichia coli to induce the regression of colorectal cancer through production of 5-aminolevulinic acid.

Microb Biotechnol 2021 09 16;14(5):2130-2139. Epub 2021 Jul 16.

State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, Shandong, 266237, China.

Bacterial vectors can be engineered to generate microscopic living therapeutics to produce and deliver anticancer agents. Escherichia coli Nissle 1917 (Nissle 1917) is a promising candidate with probiotic properties. Here, we used Nissle 1917 to develop a metabolic strategy to produce 5-aminolevulinic acid (5-ALA) from glucose as 5-ALA plays an important role in the photodynamic therapy of cancers. The coexpression of hemA and hemL using a low copy-number plasmid led to remarkable accumulation of 5-ALA. The downstream pathway of 5-ALA biosynthesis was inhibited by levulinic acid (LA). Small-scale cultures of engineered Nissle 1917 produced 300 mg l of 5-ALA. Recombinant Nissle 1917 was applied to deliver 5-ALA to colorectal cancer cells, in which it induced the accumulation of antineoplastic protoporphyrin X (PpIX) and specific cytotoxicity towards colorectal cancer cells irradiated with a 630 nm laser. Moreover, this novel combination therapy proved effective in a mouse xenograft model and was not cytotoxic to normal tissues. These findings suggest that Nissle 1917 will serve as a potential carrier to effectively deliver 5-ALA for cancer therapy.
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http://dx.doi.org/10.1111/1751-7915.13894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449674PMC
September 2021

Improved cell seeding efficiency and cell distribution in porous hydroxyapatite scaffolds by semi-dynamic method.

Cell Tissue Bank 2021 Jul 12. Epub 2021 Jul 12.

China Key Laboratory of Advanced Technologies of Materials, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.

Tissue engineering is a promising technique for the repair of bone defects. An efficient and homogeneous distribution of cell seeding into scaffold is a crucial but challenging step in the technique. Murine bone marrow mesenchymal stem cells were seeded into porous hydroxyapatite scaffolds of two morphologies by three methods: static seeding, semi-dynamic seeding, or dynamic perfusion seeding. Seeding efficiency, survival, distribution, and proliferation were quantitatively evaluated. To investigate the performance of the three seeding methods for larger/thicker scaffolds as well as batch seeding of numerous scaffolds, three scaffolds were stacked to form assemblies, and seeding efficiencies and cell distribution were analyzed. The semi-dynamic seeding and static seeding methods produced significantly higher seeding efficiencies, vitalities, and proliferation than did the dynamic perfusion seeding. On the other hand, the semi-dynamic seeding and dynamic perfusion seeding methods resulted in more homogeneous cell distribution than did the static seeding. For stacked scaffold assemblies, the semi-dynamic seeding method also created superior seeding efficiency and longitudinal cell distribution homogeneity. The semi-dynamic seeding method combines the high seeding efficiency of static seeding and satisfactory distribution homogeneity of dynamic seeding while circumventing their disadvantages. It may contribute to improved outcomes of bone tissue engineering.
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http://dx.doi.org/10.1007/s10561-021-09945-5DOI Listing
July 2021

CT Quantification of COVID-19 Pneumonia at Admission Can Predict Progression to Critical Illness: A Retrospective Multicenter Cohort Study.

Front Med (Lausanne) 2021 17;8:689568. Epub 2021 Jun 17.

Department of Radiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Early identification of coronavirus disease 2019 (COVID-19) patients with worse outcomes may benefit clinical management of patients. We aimed to quantify pneumonia findings on CT at admission to predict progression to critical illness in COVID-19 patients. This retrospective study included laboratory-confirmed adult patients with COVID-19. All patients underwent a thin-section chest computed tomography (CT) scans showing evidence of pneumonia. CT images with severe moving artifacts were excluded from analysis. Patients' clinical and laboratory data were collected from medical records. Three quantitative CT features of pneumonia lesions were automatically calculated using a care.ai Intelligent Multi-disciplinary Imaging Diagnosis Platform Intelligent Evaluation System of Chest CT for COVID-19, denoting the percentage of pneumonia volume (PPV), ground-glass opacity volume (PGV), and consolidation volume (PCV). According to Chinese COVID-19 guidelines (trial version 7), patients were divided into noncritical and critical groups. Critical illness was defined as a composite of admission to the intensive care unit, respiratory failure requiring mechanical ventilation, shock, or death. The performance of PPV, PGV, and PCV in discrimination of critical illness was assessed. The correlations between PPV and laboratory variables were assessed by Pearson correlation analysis. A total of 140 patients were included, with mean age of 58.6 years, and 85 (60.7%) were male. Thirty-two (22.9%) patients were critical. Using a cutoff value of 22.6%, the PPV had the highest performance in predicting critical illness, with an area under the curve of 0.868, sensitivity of 81.3%, and specificity of 80.6%. The PPV had moderately positive correlation with neutrophil (%) ( = 0.535, < 0.001), erythrocyte sedimentation rate ( = 0.567, < 0.001), d-Dimer ( = 0.444, < 0.001), high-sensitivity C-reactive protein ( = 0.495, < 0.001), aspartate aminotransferase ( = 0.410, < 0.001), lactate dehydrogenase ( = 0.644, < 0.001), and urea nitrogen ( = 0.439, < 0.001), whereas the PPV had moderately negative correlation with lymphocyte (%) ( = -0.535, < 0.001). Pneumonia volume quantified on initial CT can non-invasively predict the progression to critical illness in advance, which serve as a prognostic marker of COVID-19.
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http://dx.doi.org/10.3389/fmed.2021.689568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245676PMC
June 2021

Investigation of the indoor 222Rn and 220Rn levels in the residential environment and estimation of the annual effective radiation dose for ordinary residents.

PLoS One 2021 24;16(6):e0253463. Epub 2021 Jun 24.

School of Public Health, Weifang Medical University, Weifang, Shandong Province, China.

To evaluate the health risk of radon and its progeny, a large amount of accurate monitoring data is needed according to the theory and practice of health risk assessment. However, the indoor radon levels in different regions in China and worldwide reveal temporal and spatial variations. In addition, the residents living in different areas follow distinct living modes. Therefore, it is recommended and accepted by many researchers to detect the radon level in local areas and subsequently conduct health risk assessments based on local detection data. In this study, 21 bedrooms of households in Weifang city were selected, and the indoor 222Rn and 220Rn levels were detected with RAD7 radon detector in winter, while the annual effective radiation dose was calculated for ordinary residents in Weifang city. Our investigation showed that the 24- and 12-hour average levels of 222Rn were 35.7±15.2 Bq/m3 and 36.2±15.8 Bq/m3, respectively. The 24- and 12-hour average levels of 220Rn were 30.4±12.3 Bq/m3 and 22.4±11.6 Bq/m3, respectively. There were significant differences in the average levels of 222Rn and 220Rn between floors. The estimated annual effective radiation dose received by ordinary residents in Weifang city was 1.7193 mSv, of which 0.9479 mSv originated from 222Rn and its progeny and 0.7714 mSv originated from 220Rn and its progeny, accounting for 55.1% and 44.9%, respectively, of the total dose. Our findings suggest that 220Rn should not be ignored by local residents in Weifang city, and more attention should be paid to 220Rn in future research.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253463PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224870PMC
November 2021

Phagocytosis checkpoints on hematopoietic stem cells in patients with myelodysplastic syndromes.

Asia Pac J Clin Oncol 2021 Jun 21. Epub 2021 Jun 21.

Department of Hematology, General Hospital, Tianjin Medical University, Tianjin, China.

Background: The myelodysplastic syndrome (MDS) is a high-risk hemocytopenia easily converted to acute myeloid leukemia. CD47 plays an important role in regulating phagocytosis, and its role in the pathogenesis of MDS is unclear.

Methods: CD47 and PI3K/AKT/mTOR on CD34 CD38 cells were detected by flow cytometry. NF-κB, PI3K, AKT, PTEN, and mTOR mRNA overexpressed in CD34 CD38 CD47 cells were performed by real-time quantitative transcriptase-polymerase chain reaction. Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres. Sorted CD34 CD38 CD47 cells were injected into NOD-Prkdc Il2rg mice.

Results: The expression of CD47 on CD34 CD38 cells of the patients in high-risk MDS based on IPSS-R/WPSS score was higher than that in low-risk MDS and controls. The signaling pathway of PI3K/AKT/mTOR is active in CD34 CD38 CD47 cells of MDS patients. CD47 overexpressing CD34 CD38 cells has antiphagocytosis. CD47 overexpressing leukemia stem cell (LSC) -transplanted mice has a short survival time. The macrophages originated from MDS might elicit a pro-tumor response in MDS by inhibiting phagocytosis.

Conclusions: Phagocytosis checkpoints are impaired in MDS. High expression of CD47 on CD34+CD38 cells indicates poor clinical prognosis in MDS.
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http://dx.doi.org/10.1111/ajco.13566DOI Listing
June 2021

Involvement of microRNA-155 in the mechanism of electroacupuncture treatment effects on experimental autoimmune encephalomyelitis.

Int Immunopharmacol 2021 Aug 3;97:107811. Epub 2021 Jun 3.

Department of Neurobiology, Harbin Medical University, No. 194 Xuefu Road, Harbin, Heilongjiang 150081, China; The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang 150081, China. Electronic address:

Multiple sclerosis (MS) is a neurodegenerative and demyelinating autoimmune disease mediated by autoreactive T cells that affects the central nervous system (CNS). Electroacupuncture (EA) has emerged as an alternative or supplemental treatment for MS, but the mechanism by which EA may alleviate MS symptoms is unresolved. Here, we examined the effects of EA at the Zusanli (ST36) acupoint on mice with experimental autoimmune encephalomyelitis (EAE), the predominant animal model of MS. The effects of EA on EAE emergence, inflammatory cell levels, proinflammatory cytokines, and spinal cord pathology were examined. EA treatment attenuated the EAE clinical score and associated spinal cord demyelination, while reducing the presence of proinflammatory cytokines in mononuclear cells (MNCs), downregulating microRNA (miR)-155, and upregulating the opioid peptide precursor proopiomelanocortin (POMC) in the CNS. Experiments in which cultured neurons were transfected with a miR-155 mimic or a miR-155 inhibitor further showed that the direct modulation of miR-155 levels could regulate POMC levels in neurons. In conclusion, the alleviation of EAE by EA is characterized by reduced proportions of Th1/Th17 cells and increased proportions of Th2 cells, POMC upregulation, and miR-155 downregulation, while miR-155 itself can suppress POMC expression. These results, support the hypothesis that the effects of EA on EAE may involve the downregulation of miR-155.
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http://dx.doi.org/10.1016/j.intimp.2021.107811DOI Listing
August 2021

Severity of COVID-19 in Cancer patients versus patients without Cancer: A Propensity Score Matching Analysis.

J Cancer 2021 24;12(12):3558-3565. Epub 2021 Apr 24.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China.

Data are extremely limited with regards to the impact of COVID-19 on cancer patients. Our study explored the distinct clinical features of COVID-19 patients with cancer. 189 COVID-19 patients, including 16 cancer patients and 173 patients without cancer, were recruited. Propensity score 1:4 matching (PSM) was performed between cancer patients and patients without cancer based on age, gender and comorbidities. Survival was calculated by the Kaplan-Meier method and the difference was compared by the log-rank test. PSM analysis yielded 16 cancer patients and 64 propensity score-matched patients without cancer. Compared to patients without cancer, cancer patients tended to have leukopenia and elevated high-sensitivity C-reactive protein (hs-CRP) and procalcitonin. For those with critical COVID-19, cancer patients had an inferior survival than those without cancer. Also, cancer patients with severe/critical COVID-19 tended to be male and present with low SO and albumin, and high hs-CRP, lactate dehydrogenase and blood urea nitrogen on admission compared to those with mild COVID-19. In terms of risk factors, recent cancer diagnosis (within 1 year of onset of COVID-19) and anti-tumor treatment within 3 months of COVID-19 diagnosis were associated with inferior survival. We found COVID-19 patients with cancer have distinct clinical features as compared to patients without cancer. Importantly, cancer patients with critical COVID-19 were found to have poorer outcomes compared to those without cancer. In the cancer cohort, patients with severe/critical COVID-19 presented with a distinct clinical profile from those with mild COVID-19; short cancer history and recent anti-cancer treatment were associated with inferior survival.
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http://dx.doi.org/10.7150/jca.54205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120166PMC
April 2021
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