Publications by authors named "Yuling Qing"

6 Publications

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Platinum-Copper Bimetallic Colloid Nanoparticle Cluster Nanozymes with Multiple Enzyme-like Activities for Scavenging Reactive Oxygen Species.

Langmuir 2021 06 7;37(24):7364-7372. Epub 2021 Jun 7.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002 Jiangsu, P. R. China.

Fabrication of high-performance artificial antioxidant enzyme (AAE) systems based on a single nanozyme possessing multi-enzymatic activities is fascinating but challenging. Here, polyvinylpyrrolidone (PVP)-platinum-copper nanoparticle clusters (PVP-PtCuNCs) are prepared by a facile one-pot chemical coreduction method. PVP-PtCuNCs possess efficient superoxide dismutase (SOD)-like, peroxidase (POD)-like, and catalase (CAT)-like activities, and the multi-enzymatic activities depend on the bimetal component and cluster structure. Compared with individual platinum nanoparticle clusters (PVP-PtNCs), PVP-PtCuNCs can effectively eliminate reactive oxygen species (ROS) including superoxide anions, hydrogen peroxide, and hydroxyl radicals. The doping of copper not only reduces the usage of Pt content but also improves the catalytic efficiency and versatility effectively through the synergistic effect of bimetal components and the nanocluster structure. The results not only demonstrate that a single bimetallic nanozyme has the potential as an efficient AAE system in the biomedical application but also demonstrate that traditional concepts of structure-activity relationships can be used to fabricate nanozymes with the desired multi-enzymatic activities.
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http://dx.doi.org/10.1021/acs.langmuir.1c00697DOI Listing
June 2021

Complete mitochondrial genome of Hepialus gonggaensis (Lepidoptera: Hepialidae), the host insect of Ophiocordyceps sinensis.

Mitochondrial DNA A DNA Mapp Seq Anal 2016 11 13;27(6):4205-4206. Epub 2015 May 13.

e Chongqing Taiji Industry (Group) Co., Ltd , Chongqing , China.

The complete mitochondrial genome sequence of Hepialus gonggaensis was sequenced for the first time. The complete mtDNA sequence was 15,940 bp in length and contained 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and an AT-rich region, the gene composition and the arrangement of which were identical to other insects of Hepialidae. The overall base composition of the heavy strand was 41.14% A, 40.24% T, 11.17% C, and 7.45% G, with an AT content of 81.37%. The necleotide sequence data of 13 protein-coding genes of H. gonggaensis and other 10 Lepidoptera species were used for constructing the phylogenetic tree. It revealed that H. gonggaensis and other four Hepialidae species were clustered to a clade with high bootstraps values.
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http://dx.doi.org/10.3109/19401736.2015.1022741DOI Listing
November 2016

Construction of an HBV DNA vaccine by fusion of the GM-CSF gene to the HBV-S gene and examination of its immune effects in normal and HBV-transgenic mice.

Vaccine 2010 Jun 27;28(26):4301-7. Epub 2010 Apr 27.

Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.

Background: The hepatitis B virus (HBV) DNA vaccine can generate both HBsAg-specific humoral and cellular immune responses. The immune response can be improved by inclusion of an adjuvant, such as the cytokine GM-CSF which is known to be a very good adjuvant.

Methods: To investigate the ability of GM-CSF to enhance HBV-DNA vaccines, we constructed the plasmids by fusion of GM-CSF gene to the HBV-S gene. Normal and HBV-transgenic mice were then immunized with these plasmids.

Results: Our results show that pCDNA3.1-GM-CSF-S induced the most powerful HBsAg-specific humoral and cellular immune response, and that it was able to overcome the non-response to HBsAg in HBV-transgenic mice. In contrast, pCDNA3.1-S-GM-CSF was able to induce only a very poor immune response.

Conclusions: When the HBV-S gene is fused to the GM-CSF gene, the immune effects of the HBV DNA vaccine both in normal and HBV-transgenic mice can be strengthened and HBV-DNA plasmids fused with GM-CSF may be useful for both preventative and therapeutic purposes.
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http://dx.doi.org/10.1016/j.vaccine.2010.04.023DOI Listing
June 2010

Novel vaccines for the treatment of chronic HBV infection based on mycobacterial heat shock protein 70.

Vaccine 2006 Feb 18;24(7):887-96. Epub 2006 Jan 18.

Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, PR China.

Immunogenic peptide-based vaccines can raise significant cellular immune responses. Although cytotoxic T lymphocytes (CTL) peptide epitopes are generally poor immunogens, heat shock protein 70 from Mycobacterium tuberculosis (TBhsp70) can overcome this problem since it is a potent adjuvant that links innate and adaptive immune responses. Our goal is to use TBhsp70 as an adjuvant for development of therapeutic vaccines for chronic Hepatitis B virus infection (HBV). To this end, we genetically fused the HBV core 18-27 peptide (HBcAg((18-27))) as a CTL epitope to the C-terminus of TBhsp70 and expressed the resulting protein in methylotropic yeast Pichia pastoris GS115. At the same time, the TBhsp70-HBcAg((18-27)) peptide complex was reconstituted in vitro. We investigated whether TBhsp70-peptide complex and TBhsp70-peptide fusion protein could generate antigen specific CTL responses in vitro. Dendritic cells (DC) from HLA-A2(+) chronic HBV infection and healthy control pulsed with two vaccines were studied phenotypically by FACS analyses and functionally by cytokine release, and HBV-specific CTL response. Our results demonstrate that two vaccines can activate DC of chronic HBV infection and healthy control by upregulation CD40 and CD86, high production of IL-12p70 and TNF-alpha. Furthermore, autologous T cells with DC stimulated by two vaccines can produce IFN-gamma and generate HBV-specific CTL response. However, capacity for CTL response and cytokines production from HBV infections remained inferior to that of healthy controls. Thus, the strategy of utilizing TBhsp70 may provide a novel design for the development of prophylactic and therapeutic vaccines.
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http://dx.doi.org/10.1016/j.vaccine.2005.12.050DOI Listing
February 2006

A Quasi species of the pre-S/S gene and mutations of enhancer II/core promoter/pre-C in mothers and their children infected with hepatitis B virus via mother-to-infant transmission.

J Infect Dis 2006 Jan 29;193(1):88-97. Epub 2005 Nov 29.

Institute of Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, People's Republic of China.

Background: Hepatitis B virus (HBV) infection has a tendency to be chronic. The quasi species of HBV in the pre-S/S gene and mutations in enhancer II (EnhII)/core promoter (CP)/pre-C of HBV were studied in asymptomatic carrier (AsC) mothers and their children with different virus loads, to gain a better understanding of the pathogenic mechanisms of HBV.

Methods: Quasi species were analyzed using an established phylogenetic tree based on the sequences of the pre-S/S gene from 3 mother-child pairs. The mutations of EnhII/CP/pre-C were studied in 15 mother-child pairs.

Results: Substitution was the main mutation model. The phylogenetic tree indicated that the pre-S/S gene in every mother-child pair had the same root. The sequences of the pre-S/S gene of each patient were somewhat different from one another, but there was limited evolutionary distance between them. The evolutionary distances between the pre-S/S gene and the base of the tree in patients with low virus loads were greater than those in patients with high virus loads. The mutations in patients with low virus loads were much more frequent than those in patients with high virus loads and were not related to age, irrespective of whether the pre-S/S gene or EnhII/CP/pre-C was considered.

Conclusions: There are HBV quasi species in the sera of AsCs, and the mutations are related to virus load and hepatitis B e antigen seroconversion, irrespective of age.
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http://dx.doi.org/10.1086/498573DOI Listing
January 2006

[Relationship between the different replication status of HBV and mutations in core promoter in mother and children infected by mother-to-infant transmission].

Zhonghua Gan Zang Bing Za Zhi 2002 Oct;10(5):358-61

Research Institute of Viral Hepatitis, Chongqing 400010, China.

Objective: To understand the relationship between the different replication status of HBV and mutations in core promoter in mother and child infected by mother-to-infant transmission.

Methods: Core promoter was amplified by PCR and cloned into pGEM-T vector with T-A cloning technique. The recombinant plasmid pGEM-PreS/S was confirmed by digestion with restriction enzyme Apa I and Sac I. Two clones were selected to sequence each patient.

Results: Every pair of mother and child had same serotype and genotype and the homology of nucleotides encoding "a" determinant was 98 to 100%. The number of mutations in core promoter in patients with high replication status was more than that of low replication status. Mutations were distributed in BCP and Kunitz-type serine protease inhibitor region mainly. This difference was not associated with mother or child.

Conclusions: The different replication status of HBV is caused by mutations in core promoter in mother and child infected by mother-to-infant transmission and seems not to be associated with the development status.
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October 2002
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