Publications by authors named "Yujie Wang"

521 Publications

Genetic determinants of metabolic biomarkers and their associations with cardiometabolic traits in Hispanic/Latino adolescents.

Pediatr Res 2021 Oct 13. Epub 2021 Oct 13.

Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Background: Metabolic regulation plays a significant role in energy homeostasis, and adolescence is a crucial life stage for the development of cardiometabolic disease (CMD). This study aims to investigate the genetic determinants of metabolic biomarkers-adiponectin, leptin, ghrelin, and orexin-and their associations with CMD risk factors.

Methods: We characterized the genetic determinants of the biomarkers among Hispanic/Latino adolescents of the Santiago Longitudinal Study (SLS) and identified the cumulative effects of genetic variants on adiponectin and leptin using biomarker polygenic risk scores (PRS). We further investigated the direct and indirect effect of the biomarker PRS on downstream body fat percent (BF%) and glycemic traits using structural equation modeling.

Results: We identified putatively novel genetic variants associated with the metabolic biomarkers. A substantial amount of biomarker variance was explained by SLS-specific PRS, and the prediction was improved by including the putatively novel loci. Fasting blood insulin and insulin resistance were associated with PRS for adiponectin, leptin, and ghrelin, and BF% was associated with PRS for adiponectin and leptin. We found evidence of substantial mediation of these associations by the biomarker levels.

Conclusions: The genetic underpinnings of metabolic biomarkers can affect the early development of CMD, partly mediated by the biomarkers.

Impact: This study characterized the genetic underpinnings of four metabolic hormones and investigated their potential influence on adiposity and insulin biology among Hispanic/Latino adolescents. Fasting blood insulin and insulin resistance were associated with polygenic risk score (PRS) for adiponectin, leptin, and ghrelin, with evidence of some degree of mediation by the biomarker levels. Body fat percent (BF%) was also associated with PRS for adiponectin and leptin. This provides important insight on biological mechanisms underlying early metabolic dysfunction and reveals candidates for prevention efforts. Our findings also highlight the importance of ancestrally diverse populations to facilitate valid studies of the genetic architecture of metabolic biomarker levels.
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http://dx.doi.org/10.1038/s41390-021-01729-7DOI Listing
October 2021

Regulation of the ThiM riboswitch is facilitated by the trapped structure formed during transcription of the wild-type sequence.

FEBS Lett 2021 Oct 13. Epub 2021 Oct 13.

Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang, 438000, P. R. China.

The ThiM riboswitch from E.coli is a typical mRNA device that modulates downstream gene expression by sensing TPP. The helix-based RNA folding theory is used to investigate its detailed regulatory behaviors in cells. This RNA molecule is transcriptionally trapped in a state with the unstructured SD sequence in the absence of TPP, which induces downstream gene expression. As a key step to turn on gene expression, formation of this trapped state (the genetic ON state), highly depends on the co-transcriptional folding of its wild-type sequence. Instead of stabilities of the genetic ON and OFF states, the transcription rate, pause and ligand levels are combined to affect the ThiM riboswitch-mediated gene regulation, which is consistent with a kinetic control model.
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http://dx.doi.org/10.1002/1873-3468.14202DOI Listing
October 2021

Reconstruction of the incremental capacity trajectories from current-varying profiles for lithium-ion batteries.

iScience 2021 Oct 10;24(10):103103. Epub 2021 Sep 10.

Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, China.

The reliable assessment of battery degradation is fundamental for safe and efficient battery utilization. As an important health diagnostic method, the incremental capacity (IC) analysis relies highly on the low-noise constant-current profiles, which violates the real-life scenarios. Here, a model-free fitting process is reported, for the first time, to reconstruct the IC trajectories from noisy or even current-varying profiles. Based on the results from overall 22 batteries with three case studies, the errors of the peak positions in the reconstructed IC trajectories can be bounded within only 0.25%. With health indicators extracted from the reconstructed IC trajectories, the state of health can be readily determined from simple linear mappings, with estimation error lower than 1% only. By enabling the IC-based methods under complex load profiles, enhanced health assessment could be implemented to improve the reliability of the power systems and further promoting a more sustainable society.
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http://dx.doi.org/10.1016/j.isci.2021.103103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487032PMC
October 2021

Intravenous thrombolysis in patients with central retinal artery occlusion: a systematic review and meta-analysis.

J Neurol 2021 Oct 9. Epub 2021 Oct 9.

Department of Ophthalmology, The First Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang, 110001, People's Republic of China.

Background And Purpose: To investigate the efficacy and safety of intravenous thrombolysis (IVT) with alteplase in patients with central retinal artery occlusion (CRAO).

Methods: We searched the database of PubMed and EMBASE for potentially eligible studies that reported IVT in CRAO patients from their dates of inception to May 19, 2021. The rate and odds ratios (OR) of best-corrected visual acuity (BCVA) improvement, mean difference (MD) of BCVA with 95% confidence interval (CI) were pooled with random effects model.

Results: We included 8 studies enrolling 316 CRAO patients, among them, 157 patients received IVT with alteplase while 159 patients did not. The rate of best BCVA improvement was 47% (95% CI 33-62%) in the CRAO patients treated with IVT, which was higher than that of 12% (95% CI 1-23%) in those without IVT (OR 5.97, 95% CI [2.77-12.86]). In the setting of similar baseline BCVA (MD [logMAR] 0.16, 95% CI [- 0.15 to 0.46]), compared with those who did not receive IVT, the CRAO patients who received IVT had better best BCVA (MD [logMAR] - 0.23, 95% CI [- 0.44 to - 0.02]), but had no significant better final BCVA (MD [logMAR] - 0.10, 95% CI [- 0.32 to 0.12]). Two CRAO patients had complicated symptomatic intracranial hemorrhage after IVT.

Conclusions: IVT treatment might be effective and safe for CRAO patients, but ocular-specific complications which were not associated with thrombolysis could affect final visual acuity.
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http://dx.doi.org/10.1007/s00415-021-10838-6DOI Listing
October 2021

Brain and Muscle Metabolic Changes by FDG-PET in Stiff Person Syndrome Spectrum Disorders.

Front Neurol 2021 17;12:692240. Epub 2021 Sep 17.

Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

To report clinical characteristics and fluorodeoxyglucose positron emission tomography (FDG-PET) findings in the brain and muscles of individuals with stiff person syndrome (SPS) spectrum disorders (SPSSDs). Retrospective cohort study from 1997 to 2018 at Johns Hopkins Hospital identified 170 individuals with SPS or cerebellar ataxia (CA) associated with anti-glutamic acid decarboxylase (anti-GAD)-65 antibodies. Fifty-one underwent FDG-PET, with 50 involving the body and 30 with dedicated brain acquisition. The clinical and immunological profiles were extracted via medical record review. The brain scans were analyzed quantitatively using the NeuroQ software, with comparison with an averaged normal database. The body scans were reviewed qualitatively by a blinded nuclear medicine radiologist. Mean age of symptom onset was 41.5 years (range 12-75 years). Majority were female (68%) and White (64%). Of the patients, 82% had SPS (majority being classic phenotype), and 18% had CA. Three had a paraneoplastic process. Forty-seven had serum anti-GAD, two with anti-amphiphysin, and one with anti-glycine receptor antibodies. Brain metabolic abnormalities were seen in both SPS and CA, with significant differences between the groups noted in the right superior frontal cortex, right sensorimotor cortex, left inferior parietal cortex, bilateral thalami, vermis, and left cerebellum. Of the patients, 62% demonstrated muscle hypermetabolism, most commonly bilateral, involving the upper extremities or axial muscles. Neither brain nor muscle metabolism was correlated with functional outcomes nor treatments. Metabolic changes as seen by FDG-PET are present in the brain and muscle in many individuals with SPSSD. Future studies are needed to assess whether FDG-PET can help aid in the diagnosis and/or monitoring of individuals with SPSSD.
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http://dx.doi.org/10.3389/fneur.2021.692240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484315PMC
September 2021

The risk factors of old silent brain infarctions in carotid artery stenosis.

Neurol Sci 2021 Sep 29. Epub 2021 Sep 29.

Department of Neurology, People's Hospital, China Medical University, Shenhe District, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.

Background And Purpose: This study aims to investigate the risk factors of old ipsilateral silent brain infarctions (iSBIs) in patients with internal carotid artery (ICA) stenosis.

Methods: Consecutive patients with unilateral ICA stenosis ≥ 50% or occlusion were retrospectively enrolled. Old iSBIs were assessed on magnetic resonance imaging. Baseline characteristics and imaging features were compared between patients with and without iSBIs. Multivariable regression analysis was conducted to identify independent risk factors of iSBIs.

Results: We enrolled 274 patients with unilateral ICA stenosis ≥ 50% or occlusion. One hundred thirty-three patients had iSBIs to stenosed ICA (48.54%). One hundred thirty-seven patients had recent brain infarction on either side (50.0%). The patients with iSBIs had a lower level of high-density lipoprotein [OR 0.60, 95% CI (0.36-0.96), P = 0.045], higher prevalence of irregular/ulcer plaque [OR 10.71, 95% CI (4.60-24.95), P < 0.0001], and incompleteness of circle of Willis [OR 0.27, 95% CI (0.16-0.45), P < 0.0001] in all enrolled patients. In the patients without recent infarction on either side, lower level of high-density lipoprotein [OR 0.42, 95% CI (0.27-0.88), P = 0.031], higher prevalence of irregular/ulcer plaque [OR 12.73, 95% CI (4.13-39.22), P < 0.0001] and incompleteness of circle of Willis [OR 0.24, 95% CI (0.11-0.50), P = 0.004] were independently associated with iSBIs. The results were similar in ICA stenosis patients with recent brain infarction on either side.

Conclusion: In patients with carotid stenosis, incompleteness of circle of Willis, irregular/ulcer plaque, and lower level of high-density lipoprotein were independently associated with old iSBIs.
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http://dx.doi.org/10.1007/s10072-021-05638-9DOI Listing
September 2021

Spatial-Spectral Unified Adaptive Probability Graph Convolutional Networks for Hyperspectral Image Classification.

IEEE Trans Neural Netw Learn Syst 2021 Sep 23;PP. Epub 2021 Sep 23.

In hyperspectral image (HSI) classification task, semisupervised graph convolutional network (GCN)-based methods have received increasing attention. However, two problems still need to be addressed. The first is that the initial graph structure in the GCN-based methods is not sufficiently flexible to encode the homogenous structure similarity of HSI pixels when facing the complex scenarios induced by the spatial variability. Another problem is that the input (graph structure) and output (output features) of the GCN-based methods are separated with a ``single pass'' procedure, which is a suboptimal problem for HSI classification because it does not flexibly optimize the graph construction with a feedback method via output features. In this article, a novel spatial-spectral unified adaptive probability GCN (SSAPGCN) method is proposed for HSI classification. First, considering the homogeneous structural similarity of the pairwise relationships of HSI pixels, this article combines the inherent spectral information and spatial coordinates to obtain the spatial-spectral adaptive probability graph (SSAPG) structure, which can capture the probabilistic connectivity between each pair of the homogeneous HSI pixels. Second, the SSAPG structure and GCN model are combined into a unified framework to a daptively learn both the graph structure and the output features simultaneously with feedback. Finally, the proposed SSAPGCN method with two layers is evaluated on four public HSI datasets to demonstrate its superiority over different classification methods in terms of two evaluation metrics, the overall accuracy (OA) and kappa coefficient (KC), especially with small training sample sizes.
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http://dx.doi.org/10.1109/TNNLS.2021.3112268DOI Listing
September 2021

Construction and Validation of a Novel Immunosignature for Overall Survival in Uveal Melanoma.

Front Cell Dev Biol 2021 6;9:710558. Epub 2021 Sep 6.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, and immune infiltration plays a crucial role in the prognosis of UM. This study aimed to generate an immunological marker-based predictive signature for the overall survival (OS) of UM patients.

Methods: Single-sample gene-set enrichment analysis (ssGSEA) was used to profile immune cell infiltration in 79 patients with UM from The Cancer Genome Atlas (TCGA) database. Univariate and multivariate least absolute shrinkage and selection operator (LASSO) Cox regressions were used to determine the prognostic factors for UM and construct the predictive immunosignature. Receiver operating characteristic (ROC) curves, decision curve analysis (DCA), and calibration curves were performed to evaluate the clinical ability and accuracy of the model. In addition, the predictive accuracy was compared between the immunosignature and the Tumor, Node, Metastasis (TNM) staging system of American Joint Committee on Cancer (AJCC). We further analyzed the differences in clinical characteristics, immune infiltrates, immune checkpoints, and therapy sensitivity between high- and low-risk groups characterized by the prognostic model.

Results: Higher levels of immune cell infiltration in UM were related to a lower survival rate. Matrix metallopeptidase 12 (MMP12), TCDD inducible poly (ADP-ribose) polymerase (TIPARP), and leucine rich repeat neuronal 3 (LRRN3) were identified as prognostic signatures, and an immunological marker-based prognostic signature was constructed with good clinical ability and accuracy. The immunosignature was developed with a concordance index (C-index) of 0.881, which is significantly better than that of the TNM staging system ( < 0.001). We further identified 1,762 genes with upregulated expression and 798 genes with downregulated expression in the high-risk group, and the differences between the high- and low-risk groups were mainly in immune-related processes. In addition, the expression of most of the immune checkpoint-relevant and immune activity-relevant genes was significantly higher in the high-risk group, which was more sensitive to therapy.

Conclusion: We developed a novel immunosignature constructed by MMP12, TIPARP, and LRRN3 that could effectively predict the OS of UM.
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http://dx.doi.org/10.3389/fcell.2021.710558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450517PMC
September 2021

High-efficiency broadband achromatic metalens for near-IR biological imaging window.

Nat Commun 2021 09 21;12(1):5560. Epub 2021 Sep 21.

State Key Laboratory on Tunable laser Technology, Ministry of Industry and Information Technology Key Lab of Micro-Nano Optoelectronic Information System, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, P. R. China.

Over the past years, broadband achromatic metalenses have been intensively studied due to their great potential for applications in consumer and industry products. Even though significant progress has been made, the efficiency of technologically relevant silicon metalenses is limited by the intrinsic material loss above the bandgap. In turn, the recently proposed achromatic metalens utilizing transparent, high-index materials such as titanium dioxide has been restricted by the small thickness and showed relatively low focusing efficiency at longer wavelengths. Consequently, metalens-based optical imaging in the biological transparency window has so far been severely limited. Herein, we experimentally demonstrate a polarization-insensitive, broadband titanium dioxide achromatic metalens for applications in the near-infrared biological imaging. A large-scale fabrication technology has been developed to produce titanium dioxide nanopillars with record-high aspect ratios featuring pillar heights of 1.5 µm and ~90° vertical sidewalls. The demonstrated metalens exhibits dramatically increased group delay range, and the spectral range of achromatism is substantially extended to the wavelength range of 650-1000 nm with an average efficiency of 77.1%-88.5% and a numerical aperture of 0.24-0.1. This research paves a solid step towards practical applications of flat photonics.
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http://dx.doi.org/10.1038/s41467-021-25797-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455568PMC
September 2021

SOJNMF: Identifying multidimensional molecular regulatory modules by sparse orthogonality-regularized joint non-negative matrix factorization algorithm.

IEEE/ACM Trans Comput Biol Bioinform 2021 Sep 21;PP. Epub 2021 Sep 21.

Cancer is not only a very aggressive but also a very diverse disease. Recent advances in high-throughput omics technologies of cancer have enabled biomedical researchers to have more opportunities for studying its multi-level biological regulatory mechanism. However, there are few methods to explore the underlying mechanism of cancer by identifying its multidimensional molecular regulatory modules from the multidimensional omics data of cancer. In this paper, we propose a sparse orthogonality-regularized joint non-negative matrix factorization (SOJNMF) algorithm which can integratively analyze multidimensional omics data. This method can not only identify multidimensional molecular regulatory modules, but reduce the overlap rate of features among the multidimensional modules while ensuring the sparsity of the coefficient matrix after decomposition. Gene expression data, miRNA expression data and gene methylation data of liver cancer are integratively analyzed based on SOJNMF algorithm. Then, we obtain 238 multidimensional molecular regulatory modules. The results of permutation test indicate that different omics features within these modules are significantly correlated in statistics. Meanwhile, the results of functional enrichment analysis show that these multidimensional modules are significantly related to the underlying mechanism of the occurrence and development of liver cancer.
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http://dx.doi.org/10.1109/TCBB.2021.3114146DOI Listing
September 2021

Organophosphate Esters in Indoor Environment and Metabolites in Human Urine Collected from a Shanghai University.

Int J Environ Res Public Health 2021 Aug 31;18(17). Epub 2021 Aug 31.

School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.

In China, organophosphate esters (OPEs) are widely used in indoor environments. However, there is little information regarding the internal and external exposure of university students to OPEs. Therefore, in this study, nine OPEs and eight OPE metabolites (mOPEs) were measured in indoor dust and atmospheric PM samples from a university campus in Shanghai, as well as in urine samples collected from the university students. The total concentration of OPEs in the indoor dust in female dormitories (1420 ng/g) was approximately twice that in male dormitories (645 ng/g). In terms of indoor PM, the highest OPE concentration was found in meeting rooms (105 ng/m, on average), followed by chemical laboratories (51.2 ng/m), dormitories (44.9 ng/m), and offices (34.9 ng/m). The total concentrations of the eight mOPEs ranged from 279 pg/mL to 14,000 pg/mL, with a geometric mean value of 1590 pg/mL. The estimated daily intake values based on the indoor dust and PM OPE samples (external exposure) were 1-2 orders of magnitude lower than that deduced from the concentration of urinary mOPEs (internal exposure), indicating that dermal contact, dust ingestion, and inhalation do not contribute significantly to OPE exposure in the general population. Moreover, additional exposure routes lead to the accumulation of OPEs in the human body.
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http://dx.doi.org/10.3390/ijerph18179212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431728PMC
August 2021

Hypoxia-induced DEC1 mediates trophoblast cell proliferation and migration via HIF1α signaling pathway.

Tissue Cell 2021 Aug 12;73:101616. Epub 2021 Aug 12.

Department of Clinical Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address:

In early pregnancy, hypoxia is a typical extrinsic factor that regulates EVT functions including proliferation, migration and invasion which are essential for a successful pregnancy. Human differentiated embryonic chondrocyte-expressed gene 1 (DEC1), a hypoxia-regulated gene, has been reported to be overexpressed in several types of cancers. Given that the placenta and the cancer share several similarities with respect to their capacity to proliferate and invade adjacent tissues, we focused on the role of DEC1 on trophoblast function in a physiologically hypoxic environment, which may be associated with unexplained recurrent spontaneous abortion (URSA).In our study, we measured the expression of HIF-1α and DEC1 in first-trimester villi through real-time-PCR (RT-PCR) and immunohistochemical analysis. in vitro, DEC1 expression was downregulated in trophoblast cells via DEC1-specific shRNA plasmid transfection. The expression of DEC1 and HIF-1α was detected via western blotting and RT-PCR analysis. The proliferation and migration of HTR-8/SVneo cells were assayed using CCK-8 and Transwell migration assays, respectively.Our results indicated that the expression of DEC1 was significantly reduced in villi of URSA compared to that in normal pregnant women. in vitro, hypoxia induced the expression of HIF-1ɑ and DEC1 and upregulated proliferation and migration of the HTR-8/SVneo cells. Knockdown of DEC1 inhibited proliferation and migration of HTR-8/SVneo cells exposure to hypoxia. Furthermore, inhibition of HIF1α expression resulted in a significant decrease in DEC1. These findings illustrate that hypoxia-induced DEC1 expression promotes trophoblast cell proliferation and migration through the HIF1α signaling pathway, which plays an important role during placentation.
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http://dx.doi.org/10.1016/j.tice.2021.101616DOI Listing
August 2021

Organophosphate flame retardants, tetrabromobisphenol A, and their transformation products in sediment of e-waste dismantling areas and the flame-retardant production base.

Ecotoxicol Environ Saf 2021 Dec 31;225:112717. Epub 2021 Aug 31.

Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China; Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Key Laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:

Due to the prohibition of polybrominated diphenyl ethers, organophosphate flame retardants (OPFRs) and tetrabromobisphenol A (TBBPA) have become emerging flame retardants. However, knowledge about their occurrence, especially their transformation products, is still limited. This study collected sediment samples from two rivers, i.e., Lianjiang River (located at an e-waste dismantling area) and Xiaoqing River (situated at a flame retardant production base), to investigate the occurrence, composition, and spatiality distribution of OPFRs, TBBPA, and their transformation products. Both targets were detected in the Lianjiang River in the range of 220-1.4 × 10 and 108-3.1 × 10 ng/g dw (dry weight) for OPFRs and TBBPA, and 0.11-2.35 and 4.8-414 ng/g dw for their respective transformation products, respectively. The concentrations of OPFRs and TBBPA in the Xiaoqing River ranged from 4.15 to 31.5 and 0.76-2.51 ng/g dw, respectively, and no transformation products were detected. Different compositional characteristics of OPFRs and distinct spatial distribution from mainstream and tributary observed between the two rivers are attributed to the difference in the local industries. Spatial distribution and principal component analysis indicated that e-waste dismantling activities could be a vital source of local pollution. Besides, the confluence of tributaries seemed to determine the contaminant levels in the Xiaoqing River. Also, concentration ratios and Spearman's correlation between metabolites and parent chemicals were analyzed. Low concentration ratios (3.6 ×10 to 0.16) indicated a low transformation degree, and Spearman's correlation analysis suggested transformation products were partly stemming from commercial products. Considering the limited study of these transformation products, more studies on their sources, transform mechanism, and toxicity are required.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112717DOI Listing
December 2021

Detecting forest response to droughts with global observations of vegetation water content.

Glob Chang Biol 2021 Sep 3. Epub 2021 Sep 3.

Delft University of Technology, Delft, The Netherlands.

Droughts in a warming climate have become more common and more extreme, making understanding forest responses to water stress increasingly pressing. Analysis of water stress in trees has long focused on water potential in xylem and leaves, which influences stomatal closure and water flow through the soil-plant-atmosphere continuum. At the same time, changes of vegetation water content (VWC) are linked to a range of tree responses, including fluxes of water and carbon, mortality, flammability, and more. Unlike water potential, which requires demanding in situ measurements, VWC can be retrieved from remote sensing measurements, particularly at microwave frequencies using radar and radiometry. Here, we highlight key frontiers through which VWC has the potential to significantly increase our understanding of forest responses to water stress. To validate remote sensing observations of VWC at landscape scale and to better relate them to data assimilation model parameters, we introduce an ecosystem-scale analog of the pressure-volume curve, the non-linear relationship between average leaf or branch water potential and water content commonly used in plant hydraulics. The sources of variability in these ecosystem-scale pressure-volume curves and their relationship to forest response to water stress are discussed. We further show to what extent diel, seasonal, and decadal dynamics of VWC reflect variations in different processes relating the tree response to water stress. VWC can also be used for inferring belowground conditions-which are difficult to impossible to observe directly. Lastly, we discuss how a dedicated geostationary spaceborne observational system for VWC, when combined with existing datasets, can capture diel and seasonal water dynamics to advance the science and applications of global forest vulnerability to future droughts.
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http://dx.doi.org/10.1111/gcb.15872DOI Listing
September 2021

Rational Design and Development of Novel CDK9 Inhibitors for the Treatment of Acute Myeloid Leukemia.

J Med Chem 2021 Oct 3;64(19):14647-14663. Epub 2021 Sep 3.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China.

CDK9 is an essential drug target correlated to the development of acute myeloid leukemia (AML). Starting from the hit compound , which was discovered through a screening of our in-house compound library, the structural modifications were carried out based on the bioisosterism and scaffold hopping strategies. Consequently, compound displayed the optimal CDK9 inhibitory activity with an IC value of 5.41 nM, which was nearly 1500-fold higher than compound . In addition, compound exhibited significant antiproliferative activity in broad cancer cell lines. Further investigation of properties demonstrated that compound could be orally administrated with an acceptable bioavailability ( = 33.7%). In MV-4-11 subcutaneous xenograft mouse model, compound (7.5 mg/kg) could significantly suppress the tumor progression with a T/C value of 27.80%. Compound represents a promising lead compound for the development of a novel class of CDK9 inhibitors for the treatment of acute myeloid leukemia.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01148DOI Listing
October 2021

Vectorial Compound Metapixels for Arbitrary Nonorthogonal Polarization Steganography.

Adv Mater 2021 Aug 31:e2103472. Epub 2021 Aug 31.

Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, 510632, China.

Malus' law regulating the intensity of light when passed through a polarizer, forms the solid basis for image steganography based on orthogonal polarizations of light to convey hidden information without adverse perceptions, which underpins important practices in information encryptions, anti-counterfeitings, and security labels. Unfortunately, the restriction to orthogonal states being taken for granted in the common perceptions fails to advance cryptoinformation to upgraded levels of security. By introducing a vectorial compound metapixel design, arbitrary nonorthogonal polarization multiplexing of independent grayscale images with high fidelity and strong concealment is demonstrated. The Jones matrix treatment of compound metapixels consisting of double atoms with tailored in-plane orientation sum and difference allows point-by-point configuring of both the amplitude and polarization rotations of the output beam in an analytical and linear form. With this, both multiplexing two continuous grayscale images in arbitrary nonorthogonal polarization angles and concealing grayscale image on another in an arbitrary disclosure angle window are experimentally demonstrated in the visible TiO metasurface platform. The methods shed new light on multifarious metaoptics by harnessing the new degree of freedom and unlock the full potential of metasurface polarization optics.
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http://dx.doi.org/10.1002/adma.202103472DOI Listing
August 2021

Transcriptomic analysis of tumor tissues and organoids reveals the crucial genes regulating the proliferation of lung adenocarcinoma.

J Transl Med 2021 08 26;19(1):368. Epub 2021 Aug 26.

Fudan University Shanghai Cancer Center, 270 Dong-An Road, Shanghai, 200032, China.

Background: Accumulative evidence shows that an organoid is a more practical and reliable tool in cancer biology research. This study aimed to identify and validate crucial genes involved in non-small cell lung cancer carcinogenesis and development using the transcriptomic analysis of tumor tissues and organoids.

Methods: Gene set enrichment analysis (GSEA) of tumor tissues, tumor organoids, and normal tissues was performed to reveal the similar and different mechanisms involved in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) carcinogenesis and progression. Differentially expressed gene analysis, prognostic analysis, and gene co-expression network analysis were further used to identify hub genes involved in LUAD and LUSC carcinogenesis and development. Finally, LUAD cell lines and organoids were used to validate these findings.

Results: GSEA analysis was performed to reveal the similar mechanisms involved in LUAD and LUSC carcinogenesis and development, such as P53 signaling pathway, base mismatch repair, DNA replication, cAMP signaling pathway and PPAR pathway. However, comparing with LUSC organoids, LUAD organoids showed downregulation of immune-related pathways, inflammation-related pathways, MAPK signaling pathways, and Rap1 signaling pathways, although these pathways were downregulated in LUAD and LUSC tissues by comparing with normal lung tissues. Further gene co-expression network analysis and prognostic analysis indicated CDK1, CCNB2, and CDC25A as the hub tumor-promoting genes in LUAD but not in LUSC, which were further validated in other datasets. Using LUAD cell lines and organoid models, CDK1 and CCNB2 knockdown were found to suppress LUAD proliferation. However, CDC25A knockdown did not inhibit LUAD cell line proliferation but could effectively suppress LUAD organoid growth, indicating that an organoid could be used as an effective tool to study cancer biology in LUAD.

Conclusions: The results revealed CDK1, CCNB2, and CDC25A as the hub genes involved in LUAD carcinogenesis and development, which could be used as the potential biomarkers and targets for LUAD.
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http://dx.doi.org/10.1186/s12967-021-03043-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393455PMC
August 2021

Quality chemical analysis of crush-tear-curl (CTC) black tea from different geographical regions based on UHPLC-Orbitrap-MS.

J Food Sci 2021 Sep 13;86(9):3909-3925. Epub 2021 Aug 13.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui, China.

Crush-tear-curl (CTC) black tea is a popular beverage, owing to its unique taste characteristics and health benefits. However, differences in the taste quality and chemical profiles of CTC black tea from different geographical regions remain unclear. In this study, 28 CTC black tea samples were collected from six geographical regions and analyzed using electronic tongue and ultrahigh performance liquid chromatography-Orbitrap-mass spectroscopy. The e-tongue analysis indicated that each region's CTC black tea has its own relatively prominent taste characteristics: Sri Lanka (more umami and astringent), North India (more umami), China (more sweetness and astringent), South India (moderate umami and sweetness), and Kenya (moderate umami and astringent). Based on multivariate statistical analysis, 78 metabolites were tentatively identified and used as potential markers for CTC black tea of different origins, mainly including amino acids, flavone/flavonol glycosides, and pigments. Different metabolites, which contributed to the taste characteristics of CTC black tea, were clarified by partial least squares regression correlation analysis. Our findings may serve as useful references for future studies on origin traceability and quality characteristic determination of CTC black teas. PRACTICAL APPLICATION: This study provides useful references for future studies on the origin traceability and taste characteristic determination of CTC black teas from different geographical regions.
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http://dx.doi.org/10.1111/1750-3841.15871DOI Listing
September 2021

Application of natural killer cells in pancreatic cancer.

Oncol Lett 2021 Sep 8;22(3):647. Epub 2021 Jul 8.

Department of Oncology, Changhai Hospital Affiliated to Naval Military Medical University, Shanghai 200433, P.R. China.

Pancreatic cancer, a highly malignant disease, is characterized by rapid progression and early metastasis. Although the integrative treatment of pancreatic cancer has made great progress, the prognosis of patients with advanced pancreatic cancer remains extremely poor. In recent years, with the advancements in tumor immunology, immunotherapy has become a promising remedy for pancreatic cancer. Natural killer (NK) cells are the key lymphocytes in the innate immune system. NK cell function does not require antigen pre-sensitization and is not major histocompatibility complex restricted. By targeting tumors or virus-infected cells, the cells play a key role in immune surveillance. Although several questions about NK cells in pancreatic cancer still need to be further studied, there are extensive theories supporting the clinical application prospects of NK cell immunotherapy in pancreatic cancer. Since very few studies have evaluated the role of NK cells in pancreatic cancer, this review provides a comprehensive update of the role of NK cells in pancreatic cancer immunotherapy.
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http://dx.doi.org/10.3892/ol.2021.12908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299011PMC
September 2021

Speciation of Main Nutrients (N/P/K) in Hydrochars Produced from the Hydrothermal Carbonization of Swine Manure under Different Reaction Temperatures.

Materials (Basel) 2021 Jul 23;14(15). Epub 2021 Jul 23.

School of Land Resources and Environment, Key Laboratory of Agricultural Resource and Ecology in the Poyang Lake Basin of Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China.

Hydrothermal carbonization (HTC) has been proved to be a promising technology for swine manure (SM) treatment. Currently, there is a lack of systematic understanding of the transformation characteristics of nutrient speciation in the HTC of SM. In this study, the speciation of the main nutrients (N/P/K) in SM-derived hydrochar produced at different reaction temperatures (200-280 °C) was investigated. The recovery of P (61.0-67.1%) in hydrochars was significantly higher than that of N (23.0-39.8%) and K (25.5-30.0%), and the increase in reaction temperature promoted the recovery of P and reduced the recovery of N. After the HTC treatment, the percentage of soluble/available P was reduced from 61.6% in raw SM to 4.0-23.9% in hydrochars, while that of moderately labile/slow-release P was improved from 29.2% in raw SM feedstock to 65.5-82.7%. An obvious reduction was also found in the amounts of available N (from 51.3% in raw SM feedstock to 33.0-40.5% in hydrochars). The percentages of slow-release N and residual N in hydrochars produced at 240 °C reached the maximum and minimum values (46.4% and 18.9%), respectively. A total of 49.5-58.3% of K retained in hydrochars was residual (invalid) potassium. From the perspective of the mobility and availability of N, P and K only, it was suggested that the HTC of SM should be carried out at 220-240 °C. Compared with the original SM, it is safer and more effective to use the SM-derived hydrochar as an organic fertilizer.
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http://dx.doi.org/10.3390/ma14154114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347720PMC
July 2021

Tanshinone IIA alleviates vitiligo by suppressing AKT mediated CD8  T cells activation in a mouse model.

Dermatol Ther 2021 Sep 12;34(5):e15086. Epub 2021 Aug 12.

Yidu Central Hospital of Weifang, Qingzhou, China.

Tanshinone IIA has been reported to exhibit anti-inflammatory effects, while it is not clear whether Tanshinone IIA has protective role in vitiligo. Premelanosome (PMEL) CD8 T cells were adoptive transferred into Krt14- Kitl* mice with Kit ligand (KITL) over-expressed, to construct the vitiligo model. Pdk1 and Stat3 mice were crossed with Cd8 mice to establish Pdk1 and Stat3 mice. Tanshinone IIA (200 μg) was intravenous injected to treat vitiligo in mice every 3 days. The accumulation of macrophages and CD8 T cells in the ear skin was assayed by flow cytometry. Bone marrow-derived macrophages (BMDMs) were induced and stimulated with lipopolysaccharides (LPS) and IL-4. It was found that Tanshinone IIA alleviated the development of vitiligo, impaired PMEL CD8 T cells accumulation in the ear skin, and inhibited LPS-induced TNF-α, IL-6, and IL-1β expression and secretion in BMDMs, which could also inhibit IL-4-induced Arg-1 and Mrc-1 expression in BMDMs. In addition, Tanshinone IIA could inhibit the proliferation and cytotoxic function of CD8 T cells indicated by the expression of Perforin, Granzymeb, and IFN-γ. Furthermore, Tanshinone IIA treated Pdk1 mice, not Stat3 mice, showed impaired PMEL CD8 T cells accumulation in the ear skin. In summary, Tanshinone IIA alleviates vitiligo development with impaired CD8 T cells accumulation and activation of Pdk1-Akt pathway.
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http://dx.doi.org/10.1111/dth.15086DOI Listing
September 2021

The prevalence of sarcopenia and relationships between dietary intake and muscle mass in head and neck cancer patients undergoing radiotherapy: A longitudinal study.

Eur J Oncol Nurs 2021 Aug 20;53:101943. Epub 2021 Mar 20.

Department of Radiation Oncology, Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, #52 Fucheng Road, Haidian District, Beijing, 100142, China. Electronic address:

Purpose: Our study aims to investigate dietary intake characteristics and their association with skeletal muscle mass in head and neck cancer patients treated with radiotherapy.

Methods: From March 2017 to August 2018, patients with head and neck cancer who received radiotherapy at our affiliated hospital were enrolled. Dietary intake was assessed through 24-hr dietary recall and skeletal muscle mass was evaluated by bioelectrical impedance analysis at three-time points. Appendicular skeletal muscle mass was adjusted for height squared defined sarcopenia and correlated with dietary intake by generalized estimating equations (GEE).

Results: This study sample comprised 287 patients [median age: 54 years; 187 (65.2%) men]. Median dietary intake at post-treatment was 14.95 kcal/kg/day energy and 0.63 g/kg/day protein. Skeletal muscle mass decreased significantly in all patients. The prevalence of sarcopenia increased from 24.4% before treatment to 46.7% at the end of treatment. Exploratory univariate GEE analysis revealed that radiotherapy time-point, male-gender, age ≥60 and decreased dietary energy intake significantly impacted on muscle loss represented by the appendicular skeletal muscle index. After controlling covariates, dietary energy intake was only positively associated with muscle loss in women (P = 0.013, 95% CI = 0.003-0.027) but not in men (P = 0.788, 95% CI = -0.007-0.009).

Conclusion: While the loss in skeletal muscle is more prevalent in men receiving radiotherapy, the effects of dietary energy intake were only associated with women. A prospective randomized clinical trial is required to identify the appropriate amount of dietary energy supplement by gender in cancer patients treated with radiotherapy.
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http://dx.doi.org/10.1016/j.ejon.2021.101943DOI Listing
August 2021

Experimental Test of the Edwards Volume Ensemble for Tapped Granular Packings.

Phys Rev Lett 2021 Jul;127(1):018002

School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China.

Using x-ray tomography, we experimentally investigate granular packings subject to mechanical tapping for three types of beads with different friction coefficients. We validate the Edwards volume ensemble in these three-dimensional granular systems and establish a granular version of thermodynamic zeroth law. Within the Edwards framework, we also explicitly clarify how friction influences granular statistical mechanics by modifying the density of states, which allows us to determine the entropy as a function of packing fraction and friction. Additionally, we obtain a granular jamming phase diagram based on geometric coordination number and packing fraction.
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http://dx.doi.org/10.1103/PhysRevLett.127.018002DOI Listing
July 2021

Retina thickness in atypical parkinsonism: a systematic review and meta-analysis.

J Neurol 2021 Jul 10. Epub 2021 Jul 10.

Department of Ophthalmology, The First Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang, 110001, People's Republic of China.

Background And Purpose: To investigate the retina thickness assessed using optical coherence tomography in atypical parkinsonism in comparison with health controls (HC) and patients with Parkinson's disease (PD).

Methods: PubMed and EMBASE were searched for potentially eligible studies that reported retina thickness in atypical parkinsonism [including progressive supranuclear palsy (PSP), multiple system atrophy (MSA) and corticobasal degeneration] in comparison with that of HC and PD patients from their dates of inception to Jan 24, 2021. Mean difference (μm) of the thickness of peripapillary retinal nerve fiber layer (pRNFL) and central macular thickness (CMT) were pooled with random effects model.

Results: We included ten studies eligible for inclusion criteria. Average pRNFL thickness and average CMT were thinner in PSP [pooled mean difference (μm) of - 4.71, 95% CI (- 7.15, - 2.27); - 15.12, 95% CI (- 16.93, - 13.30)] and in MSA [- 5.37, 95% CI (- 6.59, - 4.15); - 5.93, 95% CI (- 11.00, - 0.87)] compared with HC, and were thinner in PSP [- 5.81, 95% CI (- 8.92, - 2.69); - 10.63, 95% CI (- 20.29, - 0.98)] and in MSA [- 0.35 μm, 95% CI (- 5.72, 5.01); - 7.42 μm [95% CI (- 12.46, - 2.38)] compared with PD. The pRNFL thickness was thinning in superior, inferior and nasal quadrants, and CMT was thinning in outer sectors in MSA compared with HC.

Conclusions: The retina thickness was significantly thinner in PSP and MSA than those in HC and PD. The specific patterns of retina thinning in MSA could be clinical importance for differentiation among atypical parkinsonism.
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http://dx.doi.org/10.1007/s00415-021-10703-6DOI Listing
July 2021

pH-Triggered Aggregation of Gold Nanoparticles for Enhanced Labeling and Long-Term CT Imaging Tracking of Stem Cells in Pulmonary Fibrosis Treatment.

Small 2021 Aug 8;17(33):e2101861. Epub 2021 Jul 8.

CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China.

Gold nanoparticles (AuNPs) pose a great challenge in the development of nanotracers that can self-adaptively alter their properties in response to certain cellular environments for long-term stem cell tracking. Herein, pH-sensitive Au nanotracers ([email protected]) are fabricated by sequential coupling of AuNPs with sulfonamide-based polymer (PSD) and cell-penetrating peptide (CPP), which can be efficiently internalized by mesenchymal stem cells (MSCs) and undergo pH-induced self-assembly in endosomes, facilitating long-term computed tomography (CT) imaging tracking MSCs in a murine model of idiopathic pulmonary fibrosis (IPF). Using the [email protected], the transplanted MSCs for the first time can be monitored with CT imaging for up to 35 days after transplantation into the lung of IPF mice, clearly elucidating the migration process of MSCs in vivo. Moreover, we preliminarily explored the mechanism of the [email protected] labeled MSCs in the alleviation of IPF, including recovery of alveolar integrity, decrease of collagen deposition, as well as down-regulation of relevant cytokine level. This work facilitates our understanding of the behavior and effect of MSCs in the therapy of IPF, thereby providing an important insight into the stem cell-based treatment of lung diseases.
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http://dx.doi.org/10.1002/smll.202101861DOI Listing
August 2021

Comparative colloidal stability, antitumor efficacy, and immunosuppressive effect of commercial paclitaxel nanoformulations.

J Nanobiotechnology 2021 Jul 5;19(1):199. Epub 2021 Jul 5.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xiannongtan Street, Beijing, 100050, People's Republic of China.

Background: Standard chemotherapy with taxanes, such as paclitaxel (PTX), remains the mainstay of systemic treatment of triple-negative breast cancer. Nanotechnology-based formulations have gradually replaced PTX injection and are widely used in China. However, no studies have compared the colloidal stability, antitumor efficacy, and safety of commercial PTX nanoformulations. Additionally, the desire to evaluate preclinical antitumor efficacy in human-derived tumor cells led to the widespread application of immunodeficient mouse models that likely contributed to the neglect of nanomedicines-immune system interactions. The present study investigated the colloidal stability, antitumor efficacy and safety, and nanomedicines-host immune system interactions of PTX nanoformulations. A further comparative analysis was performed to evaluate the clinical potential.

Results: Compared with liposome, PTX emulsion and PTX nanoparticle exhibited favorable colloidal stability. PTX emulsion was superior in inducing apoptosis and had a more pronounced inhibitory effect on 4T1-tumor spheroids compared with PTX liposome and PTX nanoparticle. Although PTX emulsion exhibited superior in vitro antitumor effect, no significant differences in the in vivo antitumor efficacy were found among the three types of PTX nanoformulations in an immunocompetent orthotopic 4T1 murine triple-negative breast cancer model. All PTX nanoformulations at maximum tolerated dose (MTD) induced lymphopenia and immunosuppression, as evidenced by the reduction of T cell subpopulations and inhibition of the dendritic cells maturation.

Conclusions: The MTD PTX nanomedicines-induced lymphopenia and immunosuppression may weaken the lymphocyte-mediated antitumor cellular immune response and partly account for the lack of differences in the in vivo antitumor outcomes of PTX nanoformulations. Understanding of what impacts PTX nanomedicines has on the immune system may be critical to improve the design and conduct of translational research of PTX nanomedicines in monotherapy or combination therapy with immunotherapy.
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http://dx.doi.org/10.1186/s12951-021-00946-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256566PMC
July 2021

Systematic review and meta-analysis of the epidemiology of vancomycin-resistance Staphylococcus aureus isolates.

Antimicrob Resist Infect Control 2021 06 30;10(1):101. Epub 2021 Jun 30.

Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Background: Vancomycin‑resistant Staphylococcus aureus (VRSA) is a serious public health challenging concern worldwide.

Objectives: Therefore, the objective of present study of 62 published studies was to evaluate the prevalence of VRSA based on different years, areas, isolate source, antimicrobial susceptibility testing, and the genetic determinants.

Methods: We searched the relevant articles that focused on the prevalence rates of VRSA in PubMed, Scopus, Embase, and Web of Science from 2000 to 2019. Statistical analyses were conducted using STATA software (version 14.0).

Results: The prevalence of VRSA was 2% before 2006, 5% in 2006-2014, and 7% in 2015-2020 that showed a 3.5-fold increase in the frequency of VRSA between before 2006 and 2020 years. The prevalence of VRSA was 5% in Asia, 1% in Europe, 4% in America, 3% in South America, and 16% in Africa. The frequencies of VRSA isolated from clinical, non-clinical, and mixed samples were 6%, 7%, and 14%, respectively. The prevalence of VRSA was 12% using disk diffusion agar method, 7% using MIC-base methods, and 4% using mixed-methods. The prevalence of vanA, vanB, and vanC1 positive were 71%, 26%, and 4% among VRSA strains. The most prevalent genotype was staphylococcal cassette chromosomemec (SCCmec) II, which accounted for 57% of VRSA. The most prevalent staphylococcal protein A (spa) types were t002, t030, and t037.

Conclusion: The prevalence of VRSA has been increasing in recent years particularly in Africa/Asia than Europe/America. The most prevalent of genetic determinants associated with VRSA were vanA and SCCmec II. This study clarifies that the rigorous monitoring of definite antibiotic policy, regular surveillance/control of nosocomial-associated infections and intensive surveillance of vancomycin-resistance are required for preventing emergence and further spreading of VRSA.
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http://dx.doi.org/10.1186/s13756-021-00967-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247230PMC
June 2021

Effects of mechanical circulatory support devices in patients with acute myocardial infarction undergoing stent implantation: a systematic review and meta-analysis of randomised controlled trials.

BMJ Open 2021 06 29;11(6):e044072. Epub 2021 Jun 29.

Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha, Hunan, China

Objective: The survival benefit of using mechanical circulatory support (MCS) in patients with acute myocardial infarction (AMI) is still controversial. It is necessary to explore the impact on clinical outcomes of MCS in patients with AMI undergoing stenting.

Design: Systematic review and meta-analysis.

Data Sources: Embase, Cochrane Library, Medline, PubMed, Web of Science, ClinicalTrials.gov and Clinicaltrialsregister.eu databases were searched from database inception to February 2021.

Eligibility Criteria: Randomised clinical trials (RCTs) on MCS use in patients with AMI undergoing stent implantation were included.

Data Extraction And Synthesis: Data were extracted and summarised independently by two reviewers. Risk ratios (RRs) and 95% CIs were calculated for clinical outcomes according to random-effects model.

Results: Twelve studies of 1497 patients with AMI were included, nine studies including 1382 patients compared MCS with non-MCS, and three studies including 115 patients compared percutaneous ventricular assist devices (pVADs) versus intra-aortic balloon pump (IABP). Compared with non-MCS, MCS was not associated with short-term (within 30 days) (RR=0.90; 95% CI 0.57 to 1.41; I=46.8%) and long-term (at least 6 months) (RR=0.82; 95% CI 0.57 to 1.17; I=37.6%) mortality reductions. In the subset of patients without cardiogenic shock (CS) compared with non-MCS, the patients with IABP treatment significantly had decreased long-term mortality (RR=0.49; 95% CI 0.27 to 0.90; I=0), but without the short-term mortality reductions (RR=0.51; 95% CI 0.22 to 1.19; I=17.9%). While in the patients with CS, the patients with MCS did not benefit from the short-term (RR=1.09; 95% CI 0.67 to 1.79; I=46.6%) or long-term (RR=1.00; 95% CI 0.75 to 1.33; I=22.1%) survival. Moreover, the application of pVADs increased risk of bleeding (RR=1.86; 95% CI 1.15 to 3.00; I=15.3%) compared with IABP treatment (RR=1.86; 95% CI 1.15 to 3.00; I=15.3%).

Conclusions: In all patients with AMI undergoing stent implantation, the MCS use does not reduce all-cause mortality. Patients without CS can benefit from MCS regarding long-term survival, while patients with CS seem not.
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http://dx.doi.org/10.1136/bmjopen-2020-044072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245450PMC
June 2021

Silencing of the chemokine CXC receptor 4 (CXCR4) hampers cancer progression and increases cisplatin (DDP)-sensitivity in clear cell renal cell carcinoma (ccRCC).

Bioengineered 2021 12;12(1):2957-2969

Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

Aberrant expression of the chemokine CXC receptor 4 (CXCR4) is closely associated with cancer progression and drug-resistance in multiple cancers, and we first investigated the role of CXCR4 in regulating cancer pathogenesis and cisplatin (DDP)-resistance in clear cell renal cell carcinoma (ccRCC) in the present study. Here, we identified that CXCR4 acted as an oncogene to promote cancer progression and genetically silencing of CXCR4 increased cisplatin (DDP)-sensitivity in ccRCC and . Functionally, analysis from the clinical and cellular data indicated that CXCR4 was significantly upregulated in ccRCC tissues and cells, compared to their normal counterparts. Next, the loss-of-function experiments validated that knock-down of CXCR4 suppressed cell proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) in ccRCC cells, while CXCR4 overexpression had opposite effects on the above cellular functions. Consistently, the xenograft tumor-bearing mice models were established, and the results supported that knock-down of CXCR4 inhibited tumor growth and the expression levels of Ki67 protein . In addition, the ccRCC cells were exposed to DDP treatment, and we surprisingly found that upregulation of CXCR4 increased DDP-resistance in ccRCC cells, and conversely, CXCR4 ablation sensitized ccRCC cells to DDP stimulation. Taken together, we concluded that CXCR4 ablation hindered cancer progression and enhanced DDP-sensitivity in ccRCC, and the present study identified a novel therapeutic biomarker for ccRCC.
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http://dx.doi.org/10.1080/21655979.2021.1943112DOI Listing
December 2021

Will the public pay for green products? Based on analysis of the influencing factors for Chinese's public willingness to pay a price premium for green products.

Environ Sci Pollut Res Int 2021 Jun 26. Epub 2021 Jun 26.

School of Economics and Management, China University of Mining and Technology, Nanhu Campus, 221116, Xuzhou, Jiangsu Province, China.

The promotion and use of green products is an important way to improve the living environment and reduce resource consumption and waste. Green products often have higher prices than general products due to its green attributes. According to the signal theory, purchasing green products can effectively convey the pro-social signals of the consumers. Therefore, based on the price premium characteristics of green products, this study constructed a theoretical and quantitative research model of the public's WTP (willingness to pay) a price premium for green products and conducted an in-depth study on the consumers' acceptability of premium for green products. A total of 991 valid questionnaires were analyzed, and the following results were obtained: (1) The public's WTP a price premium for green products was generally low, with only 30.1% of respondents. (2) The influencing factors of the WTP a price premium for green products were conditional value>green value>functional value>value expression form>price importance. Economic factors were still the main reason that hinders the public's WTP a price premium for green products. When the premium conveys public's pro-social and pro-environmental signal characteristics, it could effectively improve the public's acceptability of premium for green products. (3) The public's WTP a price premium for green products varied with marital status, education level, working years, monthly income, and occupation characteristics. The public who were married, had a master's degree or above, and had worked for 1 year or less and whose disposable monthly income was more than 50,000 yuan and whose occupation was engineers and technicians had the highest WTP a price premium for green products. (4) Policy guidance and media publicity had a positive moderating effect on the path of influencing factors on the WTP for green products. On this basis, this study proposes to deepen the exemplary leading role of the government and attach importance to the education and publicity function of green consumption consciousness. Enterprises should give full play to the influence of reference groups, highlight the value of green products, and popularize green products through appropriate price discount activities, so as to promote the public to participate more actively in the purchase of green products. At the same time, it can also provide reference and enlightenment for the formulation of relevant policies.
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http://dx.doi.org/10.1007/s11356-021-14885-4DOI Listing
June 2021
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