Publications by authors named "Yuhui Wang"

319 Publications

Metal Complexes or Chelators with ROS Regulation Capacity: Promising Candidates for Cancer Treatment.

Molecules 2021 Dec 27;27(1). Epub 2021 Dec 27.

National Local Joint Engineering Laboratory for Advanced Textile Processing and Clean Production, Wuhan Textile University, Wuhan 430073, China.

Reactive oxygen species (ROS) are rapidly eliminated and reproduced in organisms, and they always play important roles in various biological functions and abnormal pathological processes. Evaluated ROS have frequently been observed in various cancers to activate multiple pro-tumorigenic signaling pathways and induce the survival and proliferation of cancer cells. Hydrogen peroxide (HO) and superoxide anion (O) are the most important redox signaling agents in cancer cells, the homeostasis of which is maintained by dozens of growth factors, cytokines, and antioxidant enzymes. Therefore, antioxidant enzymes tend to have higher activity levels to maintain the homeostasis of ROS in cancer cells. Effective intervention in the ROS homeostasis of cancer cells by chelating agents or metal complexes has already developed into an important anti-cancer strategy. We can inhibit the activity of antioxidant enzymes using chelators or metal complexes; on the other hand, we can also use metal complexes to directly regulate the level of ROS in cancer cells via mitochondria. In this review, metal complexes or chelators with ROS regulation capacity and with anti-cancer applications are collectively and comprehensively analyzed, which is beneficial for the development of the next generation of inorganic anti-cancer drugs based on ROS regulation. We expect that this review will provide a new perspective to develop novel inorganic reagents for killing cancer cells and, further, as candidates or clinical drugs.
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http://dx.doi.org/10.3390/molecules27010148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746559PMC
December 2021

Tetrandrine alleviates podocyte injury via calcium-dependent calpain-1 signaling blockade.

BMC Complement Med Ther 2021 Dec 14;21(1):296. Epub 2021 Dec 14.

Department of Nephrology (Key laboratory of Management of Kidney Disease in Zhejiang Province), Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Tiyuchang Road 453, Hangzhou, 310007, People's Republic of China.

Background: Podocytes have become a crucial target for interventions in proteinuric kidney diseases. Many studies have reported that overexpression of transient receptor potential cation channel protein 6 (TRPC6) in podocyte injury upregulates intracellular Ca influx and stimulates Ca-dependent protease calpain-1 signaling. The traditional Chinese drug, tetrandrine, a nonselective Ca channel blocker, has long been used to treat chronic kidney disease. This research aimed to explore the possible mechanisms underlying the anti-proteinuric properties of tetrandrine.

Methods: We investigated the involvement of tetrandrine in Ca dependent calpain-1 signaling in mouse podocytes and adriamycin-induced nephropathy rats. Cyclosporine A (CsA) and U73122 were used as positive controls. Cell viability, cytotoxicity, Ca concentration, calpain activity, and mRNA and protein expression levels of calpain-1 signaling pathways were examined. The clinical and pathological changes were measured.

Results: Tetrandrine decreased intracellular Ca influx in cultured TRPC6-overexpressing podocytes. In both in vitro and in vivo studies, the administration of tetrandrine downregulated calpain activity and the expression of calpain-1 and restored the expression of downstream Talin-1 and nephrin. Compared to CsA, tetrandrine treatment exhibited superior inhibitory effects on calpain activity and calpain-1 expression.

Conclusions: Tetrandrine has therapeutic potential in podocyte damage by blocking Ca-dependent activation of the calpain-1 signaling pathway. Tetrandrine reduced proteinuria, improved renal function, and alleviate renal pathological damage.
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http://dx.doi.org/10.1186/s12906-021-03469-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670271PMC
December 2021

Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1.

Carbohydr Polym 2022 Feb 21;277:118796. Epub 2021 Oct 21.

Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, PR China; TEDA Institute of Biological Sciences and Biotechnology, Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, Tianjin, PR China. Electronic address:

Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy. Glycoconjugate vaccine represents a promising measure to reduce ExPEC infections. Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate. Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain. The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer. MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency. Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge. The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.
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http://dx.doi.org/10.1016/j.carbpol.2021.118796DOI Listing
February 2022

A persistent luminescence resonance energy transfer-based molecular beacon probe for the highly sensitive detection of microRNA in biological samples.

Biosens Bioelectron 2022 Feb 27;198:113849. Epub 2021 Nov 27.

Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences (CAS), Ningbo, 315300, PR China. Electronic address:

Herein, a time-resolved luminescence resonance energy transfer (TR-LRET) molecular beacon (MB) probe employing persistent luminescence nanoparticles (PLNPs) as the energy donors was first constructed, and further designed for microRNA21 (miR21) sensing. This probe (named as PLNPs-MB) was facilely fabricated by covalent bioconjugation between poly-(acrylic acid) (PAA) modified near-infrared (NIR) emissive PLNPs i.e. ZnGaO:Cr and functionalized MB oligonucleotide (5'-NH and 3'-BHQ3). Accordingly, PLNPs and BHQ3 were in close proximity to each other, leading to the occurrence of LRET and obvious persistent luminescence (PL) quenching. In the presence of miR21, loop of the PLNP-MB was hybridized, accompanying BHQ3 away from PLNPs and the restraint of LRET process. As a result, PL of the PLNPs was recovered, which built the foundation of miR21 quantification. The probe provided a linear response range from 0.1 to 10 nM for miR21 detection. Quantification limit of this probe was competitive and about 1-2 orders of magnitude lower than that of other reported MB probes for nucleic acid. Moreover, the proposed probe was successfully adopted for miR21 detection in biological fluids (human serum, cell extraction). This work also provided a sensitive detection nanoplatform for other targets through modifying diverse MBs onto the surface of PLNPs.
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http://dx.doi.org/10.1016/j.bios.2021.113849DOI Listing
February 2022

Comparison of three kinds of self-expandable metallic stents induced granulation tissue hyperplasia in the rabbit trachea.

Sci Rep 2021 11 30;11(1):23115. Epub 2021 Nov 30.

School of Mechanics and Engineering Science, National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, Henan, China.

To compare stent-induced granulation tissue hyperplasia of bare (SEMS), polyurethane-covered (PU-SEMS) and electrospun nanofibre-covered (EN-SEMS) self-expandable metallic stents in the rabbit trachea. Twenty-seven rabbits were randomly assigned to 3 groups that received SEMS, PU-SEMS or EN-SEMS. Computed tomography and sacrifice were performed as scheduled. Haematoxylin-eosin and Masson's trichrome staining protocols were performed for pathological analysis. The data for tracheal ventilation area ratio, qualitative histological scoring, number of epithelial layers, and thicknesses of papillary projection and submucosa were documented and statistically analysed. All stents were successfully placed under the guidance of fluoroscopy without complications. Post-stenting 3 and 7 days, computed tomography revealed that the fully expandable EN-SEMS was similar to the SEMS and PU-SEMS. The mean stented tissue score in the SEMS group was higher than those of both the PU-SEMS and EN-SEMS groups at 3 days post-stenting. The pathological findings suggested that there was no papillary projection formation 3 days after stent placement. The thickness of papillary projection in the SEMS group was significantly higher than those of the PU-SEMS and EN-SEMS groups at 7 days post-stenting. After stenting 4 weeks, the tracheal ventilation area ratio of SEMS, PU-SEMS and EN-SEMS was 0.214 ± 0.021, 0.453 ± 0.028 and 0.619 ± 0.033, respectively. There were significant between-group differences. In conclusion, the stent-induced granulation tissue formation in EN-SEMS is less severe than that of PU-SEMS and SEMS. EN-SEMS has smaller radial force, and the tracheal ventilation ratio after stent placement better than that of PU-SEMS.
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http://dx.doi.org/10.1038/s41598-021-02573-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632974PMC
November 2021

Dietary-Induced Elevations of Triglyceride-Rich Lipoproteins Promote Atherosclerosis in the Low-Density Lipoprotein Receptor Knockout Syrian Golden Hamster.

Front Cardiovasc Med 2021 2;8:738060. Epub 2021 Nov 2.

Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Institute of Cardiovascular Sciences, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, China.

Elevated triglycerides are associated with an increased risk of cardiovascular disease (CVD). Therefore, it is very important to understand the metabolism of triglyceride-rich lipoproteins (TRLs) and their atherogenic role in animal models. Using low-density lipoprotein receptor knockout (LDLR) Syrian golden hamsters, this study showed that unlike LDLR mice, when LDLR hamsters were fed a high cholesterol high-fat diet (HFD), they had very high plasma levels of triglycerides and cholesterol. We found that LDLR hamsters exhibited increased serum TRLs and the ApoB100 and 48 in these particles after being fed with HFD. Treatment with ezetimibe for 2 weeks decreased these large particles but not the LDL. In addition, ezetimibe simultaneously reduced ApoB48 and ApoE in plasma and TRLs. The expression of LRP1 did not change in the liver. These findings suggested that the significantly reduced large particles were mainly chylomicron remnants, and further, the remnants were mainly cleared by the LDL receptor in hamsters. After 40 days on an HFD, LDLR hamsters had accelerated aortic atherosclerosis, accompanied by severe fatty liver, and ezetimibe treatment reduced the consequences of hyperlipidemia. Compared with the serum from LDLR hamsters, that from ezetimibe-treated LDLR hamsters decreased the expression of vascular adhesion factors in vascular endothelial cells and lipid uptake by macrophages. Our results suggested that in the LDLR hamster model, intestinally-derived lipoprotein remnants are highly atherogenic and the inflammatory response of the endothelium and foam cells from macrophages triggered atherosclerosis. The LDL receptor might be very important for chylomicrons remnant clearance in the Syrian golden hamster, and this may not be compensated by another pathway. We suggest that the LDLR hamster is a good model for the study of TRLs-related diseases as it mimics more complex hyperlipidemia.
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http://dx.doi.org/10.3389/fcvm.2021.738060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593475PMC
November 2021

[Lipids-lowering effects of equol on low density lipoprotein receptor knockout hamsters].

Wei Sheng Yan Jiu 2021 Sep;50(5):775-780

School of Public Health, Peking University, Beijing 100191, China.

Objective: Used low density lipoprotein receptor knockout(LDLR KO) hamster as the model similar to human dyslipidemia to observe the lipid-lowering effect of equol on heterozygotes.

Methods: With soy-free high cholesterol high fat diet, 12-week-old LDLR KO female heterozygous hamsters were randomly divided into negative control group(no addition), positive control group(add 0.004% ezetimibe), genistein group(add 0.1%), and low, medium and high-dose groups of equol(add 0.025%, 0.05%, 0.1% respectively). Body weight, food consumption and blood lipid were continuously monitored for 12 weeks after feeding each group. Finally, liver morphology and lipid metabolism related genes expressions were checked.

Results: There was no significant difference in body weight and average weekly food intake among the groups. The blood lipids in negative control group increased over time, and the cholesterol and triglyceride levels of LDLR KO heterozygous hamsters were significantly reduced by ezetimibe in the second week, while the high-density lipoprotein cholesterol was also significantly decreased. The lipid-lowering effects of genistein and equol were weaker than ezetimibe, and there was significant difference between the two groups after 12 weeks, but the decrease of HDL-c was not as significant as ezetimibe. Compared with genistein, the effect of medium and high dose equol was stronger. At 12 weeks, the liver weight ratio also decreased significantly, and the liver lipid accumulation was inhibited, especially in the high dose of equol. The expression of ApoAI, SREBP-2 and HMGCR were significantly up-regulated by equol and genistein.

Conclusion: Equol could reduce female LDLR KO hamster blood lipid. It may play a role in lipid lowering by inhibiting cholesterol absorption besides estrogen receptor pathway, but it is weaker than NPC1 L1 inhibitor. At the same time, up-regulation of ApoAI inhibits the decrease of high-density lipoprotein and reduces lipid accumulation in liver.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.05.012DOI Listing
September 2021

Lipoprotein Glomerulopathy-Like Lesions in Atherosclerotic Mice Defected With HDL Receptor SR-B1.

Front Cardiovasc Med 2021 8;8:734824. Epub 2021 Oct 8.

Institute of Cardiovascular Diseases, First Affiliated Hospital of Dalian Medical University, Dalian, China.

High-density lipoprotein (HDL) homeostasis is important in maintaining both cardiovascular and renal health. Scavenger receptor class B type 1 (SR-B1), the major HDL receptor in mammals, plays a crucial role in reverse cholesterol transport and HDL metabolism. Evidence from mouse study has well demonstrated that HDL disorders caused by Srb1 inactivation accelerate atherosclerosis and even induce lethal cardiovascular diseases. However, the renal consequences of Srb1 dysfunction are still unknown. Here we explored this issue in both Srb1 knockout (Srb1-/-) mice and atherosclerotic low-density lipoprotein receptor knockout (Ldlr-/-) mice with Srb1 deletion. Our data showed that no apparent renal damage was observed in 5-month-old Srb1-/- mice fed on standard rodent chow diet as well as Srb1-/- mice fed on a high-fat diet (HFD) for 12 weeks. However, 5-month-old Srb1/Ldlr-/- mice fed on rodent chow had increased urinary albumin excretion and developed spontaneous intraglomerular Oil-red O (ORO)-positive lipoprotein deposition that is similar to lesions observed in human lipoprotein glomerulopathy (LPG). HFD feeding accelerated LPG-like lesions in Srb1/Ldlr-/- mice, inducing severe proteinuria and significantly promoting intraglomerular ORO-positive lipoprotein deposition. Interestingly, probucol reversed HFD-induced HDL disorders and almost fully abrogated LPG-like lesions in Srb1/Ldlr-/- mice. In conclusion, the present study demonstrates that SR-B1 dysfunction leads to LPG-like lesions in atherosclerotic mice, which could be rescued by probucol. SR-B1 loss-of-function mutant carriers therefore might be susceptible to developing metabolic nephropathy in addition to cardiovascular diseases, and probucol might be a potential therapeutics.
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http://dx.doi.org/10.3389/fcvm.2021.734824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531488PMC
October 2021

Enhanced waste activated sludge dewaterability by the ozone-peroxymonosulfate oxidation process: Performance, sludge characteristics, and implication.

Sci Total Environ 2022 Feb 15;807(Pt 3):151025. Epub 2021 Oct 15.

Shanghai Engineering Research Center of Solid Waste Treatment and Resource Recovery, School of Environmental Science & Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Dewatering treatment is an essential step to diminish sludge volume, cut down transportation costs, and improve subsequent disposal efficiency. In this study, ozone-peroxymonosulfate (O/PMS) oxidation process was employed to ameliorate sludge dewaterability. Sludge capillary suction time (CST) and water content (Wc) of dewatered sludge cake could reduce from 70.5 s and 81.93% to 26.7 s and 65.65%, respectively, under the optimal dosage of 30 mg/g TS O and 0.4 mmol/g TS PMS. The increased sludge zeta potential, particle size, and fluidity promoted sludge dewatering performance apparently. The decreased hydrophilic, fluorescent EPS components and proteins/peptides-like + Lipids percentage in EPS as well as the ratio of α-helix/(β-sheet + random coil) of treated EPS protein secondary structure was greatly responsible for the enhanced sludge dewaterability. SO and OH were detected in ozone-peroxymonosulfate process to crack sludge flocs, eliminate hydrophilic substances and liberate bound water. Moreover, the concentrations of both heavy metals and polycyclic aromatic hydrocarbons (PAHs) of sludge after O/PMS conditioning were decreased, and the stability and toxicity of heavy metals were also reduced, except Zn. In conclusion, this work offered a comprehensive insight based on ozone-peroxymonosulfate (O/PMS) advanced oxidation for improving the sludge dewaterability and environmental implication.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151025DOI Listing
February 2022

The effect of butylphthalide injection on the cognitive function and the TLRs/NF-κB pathway in hypertensive intracerebral hemorrhage.

Am J Transl Res 2021 15;13(8):9578-9585. Epub 2021 Aug 15.

Department of Pharmacy, People's Hospital of Anji Huzhou 313000, Zhejiang Province, China.

Objective: This study explored and analyzed the effects of butylphthalide injection on the cognitive function and on the TLRs/NF-κB pathway in hypertensive intracerebral hemorrhage patients.

Methods: A total of 115 patients admitted to our hospital with hypertensive intracerebral hemorrhages were recruited as the study cohort and randomly placed in the observation group (n=60) or the control group (n=55). In addition to the routine treatment administered in both groups, the control group was additionally administered oral nimodipine tablets, and the observation group was administered intravenous butylphthalide injections. Both groups were treated continuously for 14 days. Subsequently, the changes in the clinical efficacy, the NIHSS scores, the extremity motor function, the Fugl-Meyer scores, the blood-brain barrier (BBB) and the peripheral blood mononuclear cells (PBMCs), the TLR2, TLR9, and the NF-κB mRNA levels in the two groups before and after the treatment were compared, and any adverse reactions were observed.

Results: The total effective rate in the observation group was significantly superior to the total effective rate in the control group (). The post-treatment NIHSS scores in the two groups were dramatically lower (), and the post-treatment scores in the observation group were significantly lower than they were in the control group (). The post-treatment cerebral spinal fluid (CSF) albumin and BBB indexes in the two groups were decreased significantly compared to their pre-treatment levels (), and the post-treatment indexes in the observation group were significantly lower than the post-treatment indexes in the control group (). The post-treatment relative TLR2, TLR9, and NF-κB mRNA expressions in the two groups were apparently lower than their in pre-treatment levels (), and the relative expressions in the observation group after the treatment were clearly lower than they were in the control group ().

Conclusion: Butylphthalide injection has a high clinical efficacy in treating hypertensive intracerebral hemorrhages. The drug can effectively improve patients' cognitive functions, extremity motor functions, and BBB indexes, and its mechanism may connect with the expressive suppression of the TLRs/NF-κB signaling pathway. The treatment is safe and effective, so it is worthy of clinical promotion.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430162PMC
August 2021

Visible-light-assisted peroxymonosulfate activation by metal-free bifunctional oxygen-doped graphitic carbon nitride for enhanced degradation of imidacloprid: Role of non-photochemical and photocatalytic activation pathway.

J Hazard Mater 2022 02 29;423(Pt B):127048. Epub 2021 Aug 29.

Department of Environmental Science and Engineering, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

Bifunctional oxygen-doped graphitic carbon nitride (OCN) was fabricated to activate peroxymonosulfate (PMS) for degrading imidacloprid (IMD). The modulated electronic structure of OCN promoted the adsorption, electron transfer, and formation of the redox site of PMS. The light absorption capacity, and the separation and migration speed of photogenerated carriers of OCN were increased. Consequently, 94.5% of IMD (3.0 mg/L) was removed by OCN-10/PMS process in 2.0 h. Compared with g-CN/PMS (0.048 h), the IMD degradation rate constant of OCN-10/Vis/PMS system (1.501 h) was increased by 30.3 times. The PMS oxidation on electron-deficient C atoms and holes, the PMS reduction around electron-rich O atoms and photogenerated electrons, and the multiple reactions of superoxide radical were the sources of the main active species singlet oxygen. Moreover, even under different pH conditions, coexisting anions, humic acid, and other neonicotinoid pesticides, the OCN-10/Vis/PMS system still showed acceptable applicability. Finally, mass spectrometry identified that hydroxylation and N-dealkylation of amines were the primary degradation pathways of IMD. This paper demonstrates an environmental-friendly combined activation strategy of PMS that can be operated day and night with low energy consumption, aiming to pave the way for developing metal-free photocatalysts for high-efficient environmental purification based on advanced oxidation coupling technology.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127048DOI Listing
February 2022

Integrative Metabolomic and Transcriptomic Analyses Uncover Metabolic Alterations and Pigment Diversity in in Response to Different Nitrogen Sources.

mSystems 2021 Oct 7;6(5):e0080721. Epub 2021 Sep 7.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin, China.

Nitrogen in different chemical forms is critical for metabolic alterations in strains and associated pigment diversity. In this study, we observed that ammonium-form nitrogen was superior in promoting the biosynthesis of pigments (MPs) when compared with nitrate and organic forms. Moreover, with any nitrogen source, the production of yellow and orange pigments was highly synchronized but distantly related to red pigments. However, transcriptional analyses of MP gene clusters suggested a low contribution to MP accumulation, suggesting that MP-limiting factors were located outside the gene cluster. Our metabolomic analyses demonstrated that red pigment biosynthesis was closely related to intracellular amino acids, whereas orange and yellow pigments were associated with nucleotides. In addition, weighted gene coexpression network analyses (WGCNA) based on transcriptomic data showed that multiple primary metabolic pathways were closely related to red pigment production, while several secondary pathways were related to orange pigments, and others were involved with yellow pigment regulation. These findings demonstrate that pigment diversity in is under combined regulation at metabolomic and transcriptomic levels. Natural MPs containing a mixture of red, orange, and yellow pigments are widely used as food coloring agents. MP diversity provides foods with versatile colors and health benefits but, in turn, complicate efforts to achieve maximum yield or desirable combination of pigments during the manufacturing process. Apart from the MP biosynthetic gene cluster, interactions between the main biosynthetic pathways and other intracellular genes/metabolites are critical to our understanding of MP differentiation. The integrative multiomics analytical strategy provides a technical platform and new perspectives for the identification of metabolic shunting mechanisms in MP biosynthesis. Equally, our research highlights the influence of intracellular metabolic alterations on MP differentiation, which will facilitate the rational engineering and optimization of MP production in the future.
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http://dx.doi.org/10.1128/mSystems.00807-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547423PMC
October 2021

Fluorescent carbon-dots enhance light harvesting and photosynthesis by overexpressing PsbP and PsiK genes.

J Nanobiotechnology 2021 Aug 28;19(1):260. Epub 2021 Aug 28.

Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315300, People's Republic of China.

Background: Fluorescent carbon-dots (CDs) with multifaceted advantages have provided hope for improvement of crop growth. Near infrared (NIR) CDs would be more competitive and promising than short-wavelength emissive CDs, which are not directly utilized by chloroplast. The molecular targets and underlying mechanism of these stimulative effects are rarely mentioned.

Results: NIR-CDs with good mono-dispersity and hydrophily were easily prepared by a one-step microwave-assisted carbonization manner, which showed obvious UV absorptive and far-red emissive properties. The chloroplast-CDs complexes could accelerate the electron transfer from photosystem II (PS II) to photosystem I (PS I). NIR-CDs exhibited a concentration-dependent promotion effect on N. benthamiana growth by strengthening photosynthesis. We firstly demonstrated that potential mechanisms behind the photosynthesis-stimulating activity might be related to up-regulated expression of the photosynthesis and chloroplast synthesis related genes, among which PsbP and PsiK genes are the key regulators.

Conclusion: These results illustrated that NIR-CDs showed great potential in the applications to increase crop yields through ultraviolet light harvesting and elevated photosynthesis efficiency. This work would provide a theoretical basis for the understanding and applications of the luminescent nanomaterials (not limited to CDs) in the sunlight conversion-related sustainable agriculture.
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http://dx.doi.org/10.1186/s12951-021-01005-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403421PMC
August 2021

ILC1s and ILC3s Exhibit Inflammatory Phenotype in Periodontal Ligament of Periodontitis Patients.

Front Immunol 2021 26;12:708678. Epub 2021 Jul 26.

Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.

Innate lymphoid cells (ILCs) are emerging as important players in inflammatory diseases. The oral mucosal barrier harbors all ILC subsets, but how these cells regulate the immune responses in periodontal ligament tissue during periodontitis remains undefined. Here, we show that total ILCs are markedly increased in periodontal ligament of periodontitis patients compared with healthy controls. Among them, ILC1s and ILC3s, particularly NKp44ILC3 subset, are the predominant subsets accumulated in the periodontal ligament. Remarkably, ILC1s and ILC3s from periodontitis patients produce more IL-17A and IFN-γ than that from healthy controls. Collectively, our results highlight the role of ILCs in regulating oral immunity and periodontal ligament inflammation and provide insights into targeting ILCs for the treatment of periodontitis.
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http://dx.doi.org/10.3389/fimmu.2021.708678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350136PMC
January 2022

Evaluation of Rpf protein of Micrococcus luteus for cultivation of soil actinobacteria.

Syst Appl Microbiol 2021 Sep 17;44(5):126234. Epub 2021 Jul 17.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, PR China. Electronic address:

Rpf protein, a kind of resuscitation promoting factor, was first found in the culture supernatant of Micrococcus luteus. It can resuscitate the growth of M. luteus in "viable but non-culture, VBNC" state and promote the growth of Gram-positive bacteria with high G + C content. This paper investigates the resuscitating activity of M. luteus ACCC 41016 Rpf protein, which was heterologously expressed in E. coli, to cells of M. luteus ACCC 41016 and Rhodococcus marinonascens HBUM200062 in VBNC state, and examines the effect on the cultivation of actinobacteria in soil. The results showed that the recombinant Rpf protein had resuscitation effect on M. luteus ACCC 41016 and R. marinonascens HBUM200062 in VBNC state. 83 strains of actinobacteria, which were distributed in 9 families and 12 genera, were isolated from the experimental group with recombinant Rpf protein in the culture medium. A total of 41 strains of bacteria, which were distributed in 8 families and 9 genera, were isolated from the control group without Rpf protein. The experimental group showed richer species diversity than the control group. Two rare actinobacteria, namely HBUM206391 and HBUM206404, were obtained in the experimental group supplemented with Rpf protein. Both may be potential new species of Actinomadura and Actinokineospora, indicating that the recombinant expression of M. luteus ACCC 41016 Rpf protein can effectively promote the isolation and culture of actinobacteria in soil.
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http://dx.doi.org/10.1016/j.syapm.2021.126234DOI Listing
September 2021

Antitumor efficacy of XPO1 inhibitor Selinexor in KRAS-mutant lung adenocarcinoma patient-derived xenografts.

Transl Oncol 2021 Oct 17;14(10):101179. Epub 2021 Jul 17.

Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, Ontario M5G 1L7, Canada. Electronic address:

Gain-of-function Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations occur in 25% of lung adenocarcinomas, and these tumors are challenging to treat. Some preclinical work, largely based on cell lines, suggested KRAS lung cancers are especially dependent on the nuclear export protein exportin-1 (XPO1), while other work supports XPO1 being a broader cancer dependency. To investigate the sensitivity of KRAS lung cancers to XPO1 inhibition in models that more closely match clinical tumors, we treated 10 independently established lung cancer patient-derived tumor xenografts (PDXs) with the clinical XPO1 inhibitor, Selinexor. Monotherapy with Selinexor reduced tumor growth in all KRAS PDXs, which included 4 different codon mutations, and was more effective than the clinical MEK1/2 inhibitor, Trametinib. Selinexor was equally effective in KRAS and KRAS tumors, with TP53 mutations being a biomarker for a weaker drug response. By mining genome-wide dropout datasets, we identified XPO1 as a universal cancer cell dependency and confirmed this functionally in two KRAS PDX models harboring kinase drivers. However, targeted kinase inhibitors were more effective than Selinexor in these models. Our findings support continued investigation of XPO1 inhibitors in KRAS lung adenocarcinoma, regardless of the codon alteration.
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http://dx.doi.org/10.1016/j.tranon.2021.101179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313753PMC
October 2021

SMIX(λ): Enhancing Centralized Value Functions for Cooperative Multiagent Reinforcement Learning.

IEEE Trans Neural Netw Learn Syst 2021 Jun 28;PP. Epub 2021 Jun 28.

Learning a stable and generalizable centralized value function (CVF) is a crucial but challenging task in multiagent reinforcement learning (MARL), as it has to deal with the issue that the joint action space increases exponentially with the number of agents in such scenarios. This article proposes an approach, named SMIX(λ), that uses an off-policy training to achieve this by avoiding the greedy assumption commonly made in CVF learning. As importance sampling for such off-policy training is both computationally costly and numerically unstable, we proposed to use the λ-return as a proxy to compute the temporal difference (TD) error. With this new loss function objective, we adopt a modified QMIX network structure as the base to train our model. By further connecting it with the Q(λ) approach from a unified expectation correction viewpoint, we show that the proposed SMIX(λ) is equivalent to Q(λ) and hence shares its convergence properties, while without being suffered from the aforementioned curse of dimensionality problem inherent in MARL. Experiments on the StarCraft Multiagent Challenge (SMAC) benchmark demonstrate that our approach not only outperforms several state-of-the-art MARL methods by a large margin but also can be used as a general tool to improve the overall performance of other centralized training with decentralized execution (CTDE)-type algorithms by enhancing their CVFs.
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http://dx.doi.org/10.1109/TNNLS.2021.3089493DOI Listing
June 2021

Interactions between pyrene and heavy metals and their fates in a soil-maize (Zea mays L.) system: Perspectives from the root physiological functions and rhizosphere microbial community.

Environ Pollut 2021 Oct 18;287:117616. Epub 2021 Jun 18.

State Key Laboratory of Hydroscience and Engineering, Tsinghua University, Beijing, 100084, PR China; Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, 621010, Sichuan, PR China.

The co-occurrence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in agricultural soils has become a worldwide food crop security concern. Pot experiments, rhizosphere microbial metagenomic sequencing, and root metatranscriptomic sequencing were performed to investigate the interactions among pyrene, Cu, and Cd in a soil-maize (Zea mays L.) system. This study provided direct evidence that the co-presence of PAHs and heavy metals changed the root physiological functions and the rhizosphere microbial community, which subsequently influenced the fate of the contaminants. Co-contamination at low levels tended to enhance the uptake potential and biodegradation performance of the plant, whereas increased contaminant concentrations produced opposite effects. The co-presence of 1000 mg/kg Cu decreased the abundance of Mycobacterium in the rhizosphere and reduced pyrene degradation by 12%-16%. The presence of 400-750 mg/kg pyrene altered the metabolic processes, molecular binding functions, and catalytic activity of enzymes in the maize roots, thus impeding the phytoextraction of Cu and Cd. Competitive absorption between Cu and Cd was observed for the 800-1000 mg/kg Cu and 50-100 mg/kg Cd co-treatment, in which Cu showed a competitive advantage, enhancing its root-to-shoot translocation. These findings provide important information for the production of safe crops and for the development of phytoremediation technologies.
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http://dx.doi.org/10.1016/j.envpol.2021.117616DOI Listing
October 2021

Gender Differences between Cyberbullying Victimization and Meaning in Life: Roles of Fatalism and Self-Concept Clarity.

J Interpers Violence 2021 Jun 25:8862605211028285. Epub 2021 Jun 25.

School of Education, Renmin University of China, Beijing, China.

Despite widely linking cyberbullying victimization (CV) to some poorer mental health outcomes, CV also negatively impacts the internal strengths that make life worth living, such as meaning in life, which has received less attention. This study thus aimed to examine the relationship between CV and presence of meaning in life as well as the mediating roles of fatalism and self-concept clarity in this relationship. Gender differences in the mediation model were also examined. A sample of 766 Chinese junior school students ( = 13.11 years, = 1.19 years) completed questionnaires regarding CV, presence of meaning, self-concept clarity, and fatalism. The results revealed that CV was significantly and positively correlated with presence of meaning. Structural equation modeling indicated that self-concept clarity and fatalism completely mediated the link between CV and presence of meaning in parallel and sequential manners. The multigroup analysis further showed that CV was positively related to fatalism only among girls and had a stronger negative association with self-concept clarity for girls compared with boys. Thus, the indirect link between CV and presence of meaning was stronger for girls (versus boys). Findings suggested that CV was associated with poor self-concept clarity, stronger fatalism, and low levels of presence of meaning in life among adolescents, especially for girls. Increasing self-concept clarity and decreasing fatalistic beliefs are thus necessary to help cyberbullying victims to develop meaning in life.
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http://dx.doi.org/10.1177/08862605211028285DOI Listing
June 2021

Risk factors and incidence of third trimester stillbirths in China.

Sci Rep 2021 06 16;11(1):12701. Epub 2021 Jun 16.

Obstetrical Department, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.

About 2.6 million third-trimester stillbirths occur annually worldwide, mostly in low- and middle-income countries. However, the causes of stillbirths are rarely investigated. We performed a retrospective, hospital-based study in Zhejiang Province, southern China, of the causes of third-trimester stillbirths. Causes of stillbirths were classified using the Relevant Condition at Death classification system. From January 1, 2013, to December 31, 2018, we enrolled 341 stillbirths (born to 338 women) from 111,275 perinatal fetuses (born to 107,142 women), as well as 293 control cases (born to 291 women). The total incidence of third-trimester stillbirths was 3.06/1000 (341/111,275). There were higher proportions of women with a high body mass index, twins, pregnancy-induced hypertension, assisted reproduction and other risk factors among the antepartum than the control cases. The antepartum stillbirth fetuses were of lower median birth weight and gestational age and had a smaller portion of translucent amniotic fluid than the control cases. The antepartum stillbirth fetuses had a higher frequency of abnormalities detected prenatally and of fetal growth restriction than the control cases. Of 341 cases (born to 338 mothers), the most common causes of stillbirth were fetal conditions [117 (34.3%) cases], umbilical cord [88 (25.8%)], maternal conditions [34 (10.0%)], placental conditions [31 (9.1%)], and intrapartum [28 (8.2%)]. Only eight (2.3%), three (0.9%), and two (0.6%) stillbirths were attributed to amniotic fluid, trauma, and uterus, respectively. In 30 (8.8%) cases, the cause of death was unclassified. In conclusion, targeted investigation can ascertain the causes of most cases of third-trimester stillbirths.
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http://dx.doi.org/10.1038/s41598-021-92106-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209053PMC
June 2021

Social Comparison Orientation and Cyberbullying Perpetration and Victimization: Roles of Envy on Social Networking Sites and Body Satisfaction.

J Interpers Violence 2021 Jun 6:8862605211023486. Epub 2021 Jun 6.

Renmin University of China, Haidian District, Beijing, China.

Although some studies have explored the psychological and behavioral outcomes of social comparison orientation (SCO), few have explored the associations of SCO with cyberbullying perpetration and victimization. The current study aimed to investigate the associations of SCO with cyberbullying perpetration and victimization, and considered envy on social networking sites (SNS) as a mediator and body satisfaction as a moderator in these relations. To test our expectations, 941 adolescents aged between 10 and 16 years ( = 13.15, SD = 1.18) completed multiple questionnaires, namely the Social Comparison Orientation Scale, the Revised Cyber Bullying Inventory, an envy scale revised for the SNS context, and the Body Areas Satisfaction Scale. Correlation analysis revealed that cyberbullying perpetration and cyberbullying victimization were significantly and positively correlated with SCO and envy on SNS, and significantly and negatively correlated with body satisfaction. The results of structural equation modeling (SEM) revealed that envy on SNS played a mediating role in the associations of SCO with cyberbullying perpetration and victimization. The results of SEM and simple slope test analysis indicated that body satisfaction weakened the direct association of SCO with envy on SNS and further weakened the indirect associations of SCO with cyberbullying perpetration and victimization via envy on SNS. Specifically, individuals with high levels of SCO were more likely to feel envious on SNS, and further tended to bully others and be bullied online when they were dissatisfied with their bodies. In other words, positive body satisfaction protected individuals with high levels of SCO from cyberbullying perpetration and victimization by decreasing their feelings of envy on SNS.
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http://dx.doi.org/10.1177/08862605211023486DOI Listing
June 2021

A Novel Constructed Wetland Combined with Microbial Desalination Cells and its Application.

Microb Ecol 2021 Jun 4. Epub 2021 Jun 4.

College of Marine Ecology and Environment, Engineering Research Center for Water Environment Ecology in Shanghai, Shanghai Ocean University, Hucheng Ring Road 999, Office B207, Pudong District, Shanghai, 201306, China.

Wastewater recycling can alleviate the shortage of water resources. Saline water is seldom treated with biological processes, and its recycling rate is low. Constructed wetland (CW) is a safe, economical, and ecological water treatment method. However, the saline water treatment performance of CW is not good. Microbial desalination cells (MDC) utilizing a bioelectrochemical approach achieve functions of desalination and power generation. In this study, MDC was used to strengthen CW to form a composite system, MDC-CW. Through optimization of design parameters, MDC-CW was applied in the treatment of salt-containing water. The average total nitrogen removal rate in MDC-CW-P1 reached 87.33% and the average COD removal rate was 92.79%. The average desalination rate of MDC-CW-P1 was 55.78% and the average voltage of MDC-CW-P1 reached 0.40 mV. Planting Canna indica in the MDC-CW was conducive to the functions of desalination and power generation. The above results were also verified by the microbial analysis results of gravels in the substrate, plant rhizosphere, and electrodes. In addition, the decontamination of the device mainly depended on the function of the bacteria commonly used in water treatment, such as Proteobacteria and Bacteroidetes, whereas the generation of power depended on the function of Geobacter. Salt ions moved spontaneously to the cathode and anode under the influence of current generation so that the desalination function was realized under the selective isolation function of exchange membranes. The device design and laboratory applications of MDC-CW experimentally achieved the electrochemical function and broadened the treatment scale of CW.
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http://dx.doi.org/10.1007/s00248-021-01752-5DOI Listing
June 2021

Correction of Familial LCAT Deficiency by AAV-hLCAT Prevents Renal Injury and Atherosclerosis in Hamsters-Brief Report.

Arterioscler Thromb Vasc Biol 2021 07 13;41(7):2141-2148. Epub 2021 May 13.

Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, School of Basic Medical Sciences, Peking University, Beijing, China (M.G., W.H., Y.W., G.L., X.X.).

[Figure: see text].
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http://dx.doi.org/10.1161/ATVBAHA.120.315719DOI Listing
July 2021

A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes.

Nat Commun 2021 05 11;12(1):2623. Epub 2021 May 11.

Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, People's Republic of China.

COVID-19 pandemic caused by SARS-CoV-2 constitutes a global public health crisis with enormous economic consequences. Monoclonal antibodies against SARS-CoV-2 can provide an important treatment option to fight COVID-19, especially for the most vulnerable populations. In this work, potent antibodies binding to SARS-CoV-2 Spike protein were identified from COVID-19 convalescent patients. Among them, P4A1 interacts directly with and covers majority of the Receptor Binding Motif of the Spike Receptor-Binding Domain, shown by high-resolution complex structure analysis. We further demonstrate the binding and neutralizing activities of P4A1 against wild type and mutant Spike proteins or pseudoviruses. P4A1 was subsequently engineered to reduce the potential risk for Antibody-Dependent Enhancement of infection and to extend its half-life. The engineered antibody exhibits an optimized pharmacokinetic and safety profile, and it results in complete viral clearance in a rhesus monkey model of COVID-19 following a single injection. These data suggest its potential against SARS-CoV-2 related diseases.
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http://dx.doi.org/10.1038/s41467-021-22926-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113581PMC
May 2021

Structural basis for SARS-CoV-2 neutralizing antibodies with novel binding epitopes.

PLoS Biol 2021 05 7;19(5):e3001209. Epub 2021 May 7.

MOE Key Laboratory of Protein Science & Collaborative Innovation Center of Biotherapy, School of Medicine, Tsinghua University, Beijing, China.

The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) threatens global public health and economy unprecedentedly, requiring accelerating development of prophylactic and therapeutic interventions. Molecular understanding of neutralizing antibodies (NAbs) would greatly help advance the development of monoclonal antibody (mAb) therapy, as well as the design of next generation recombinant vaccines. Here, we applied H2L2 transgenic mice encoding the human immunoglobulin variable regions, together with a state-of-the-art antibody discovery platform to immunize and isolate NAbs. From a large panel of isolated antibodies, 25 antibodies showed potent neutralizing activities at sub-nanomolar levels by engaging the spike receptor-binding domain (RBD). Importantly, one human NAb, termed PR1077, from the H2L2 platform and 2 humanized NAb, including PR953 and PR961, were further characterized and subjected for subsequent structural analysis. High-resolution X-ray crystallography structures unveiled novel epitopes on the receptor-binding motif (RBM) for PR1077 and PR953, which directly compete with human angiotensin-converting enzyme 2 (hACE2) for binding, and a novel non-blocking epitope on the neighboring site near RBM for PR961. Moreover, we further tested the antiviral efficiency of PR1077 in the Ad5-hACE2 transduction mouse model of COVID-19. A single injection provided potent protection against SARS-CoV-2 infection in either prophylactic or treatment groups. Taken together, these results shed light on the development of mAb-related therapeutic interventions for COVID-19.
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http://dx.doi.org/10.1371/journal.pbio.3001209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133496PMC
May 2021

A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification.

Bio Protoc 2021 Apr 5;11(7):e3971. Epub 2021 Apr 5.

College of Tropical Crops, Hainan University, Haikou, Hainan, China.

CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression cassette(s) directly into binary vectors ( Liu , 2020 ). In this approach, we have optimized the multiplex PCR to produce an overlapping PCR product in a single reaction to generate the sgRNA expression cassette. We also amplified two sgRNA expression cassettes through a single round of PCR. Then, the sgRNA expression cassette(s) is cloned into the binary vectors in a Gateway LR or Golden gate reaction. The system reported here provides a much more efficient and simpler procedure to construct expression clones for CRISPR/Cas9-mediated genome editing. In this protocol, we describe the detailed step-by-step instructions for using this system.
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http://dx.doi.org/10.21769/BioProtoc.3971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054173PMC
April 2021

Structural basis for the regulation of inducible nitric oxide synthase by the SPRY domain-containing SOCS box protein SPSB2, an E3 ubiquitin ligase.

Nitric Oxide 2021 09 20;113-114:1-6. Epub 2021 Apr 20.

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China; Guangdong Provincial Key Laboratory of Bioengineering Medicine, Guangzhou, 510632, China; Guangdong Provincial Biotechnology Drug and Engineering Technology Research Center, Guangzhou, 510632, China; National Engineering Research Center of Genetic Medicine, Guangzhou, 510632, China. Electronic address:

Relatively high concentration of nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) in response to a variety of stimuli is a source of reactive nitrogen species, an important weapon of host innate immune defense. The SPRY domain-containing SOCS box protein 2 (SPSB2) is an E3 ubiquitin ligase that regulates the lifetime of iNOS. SPSB2 interacts with the N-terminal region of iNOS via a binding site on the SPRY domain of SPSB2, and recruits an E3 ubiquitin ligase complex to polyubiquitinate iNOS, leading to its proteasomal degradation. Although critical residues for the SPSB2-iNOS interaction have been identified, structural basis for the interaction remains to be explicitly determined. In this study, we have determined a crystal structure of the N-terminal region of iNOS in complex with the SPRY domain of SPSB2 at 1.24 Å resolution. We have resolved the roles of some flanking residues, whose contribution to the SPSB2-iNOS interaction was structurally unclear previously. Furthermore, we have evaluated the effects of SPSB2 inhibitors on NO production using transient transfection and cell-penetrating peptide approaches, and found that such inhibitors can elevate NO production in RAW264.7 macrophages. These results thus provide a useful basis for the development of potent SPSB2 inhibitors as well as recruiting ligands for proteolysis targeting chimera (PROTAC) design.
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http://dx.doi.org/10.1016/j.niox.2021.04.004DOI Listing
September 2021

Seipin Deficiency Accelerates Heart Failure Due to Calcium Handling Abnormalities and Endoplasmic Reticulum Stress in Mice.

Front Cardiovasc Med 2021 11;8:644128. Epub 2021 Mar 11.

Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.

deficiency can induce hypertrophic cardiomyopathy and heart failure, which often leads to death in humans. To explore the effects and the possible mechanisms of deficiency in myocardial remodeling, knockout (SKO) mice underwent transverse aortic constriction (TAC) for 12 weeks. We found a more severe left ventricular hypertrophy and diastolic heart failure and increases in inflammatory cell infiltration, collagen deposition, and apoptotic bodies in the SKO group compared to those in the wild type (WT) group after TAC. Electron microscopy also showed a more extensive sarcoplasmic reticulum expansion, deformation of microtubules, and formation of mitochondrial lesions in the cardiomyocytes of SKO mice than in those of WT mice after TAC. Compared with the WT group, the SKO group showed increases in endoplasmic reticulum (ER) stress-, inflammation-, and fibrosis-related gene expression, while calcium ion-related factors, such as and , were decreased in the SKO group after TAC. Increased levels of the ER stress-related protein GRP78 and decreased SERCA2a and P-RYR protein levels were detected in the SKO group compared with the WT group after TAC. Slowing of transient Ca current decay and an increased SR Ca content in myocytes were detected in the cardiomyocytes of SKO mice. Adipose tissue transplantation could not rescue the cardiac hypertrophy after TAC in SKO mice. In conclusion, we found that deficiency could promote cardiac hypertrophy and diastolic heart failure after TAC in mice. These changes may be related to the impairment of myocardial calcium handling, ER stress, inflammation, and apoptosis.
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http://dx.doi.org/10.3389/fcvm.2021.644128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990891PMC
March 2021

Value of CT features for predicting EGFR mutations and ALK positivity in patients with lung adenocarcinoma.

Sci Rep 2021 03 11;11(1):5679. Epub 2021 Mar 11.

Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Rd, Wuhan, Hubei Province, 430022, The People's Republic of China.

The aim of this study was to identify the relationships of epidermal growth factor receptor (EGFR) mutations and anaplastic large-cell lymphoma kinase (ALK) status with CT characteristics in adenocarcinoma using the largest patient cohort to date. In this study, preoperative chest CT findings prior to treatment were retrospectively evaluated in 827 surgically resected lung adenocarcinomas. All patients were tested for EGFR mutations and ALK status. EGFR mutations were found in 489 (59.1%) patients, and ALK positivity was found in 57 (7.0%). By logistic regression, the most significant independent prognostic factors of EGFR effective mutations were female sex, nonsmoker status, GGO air bronchograms and pleural retraction. For EGFR mutation prediction, receiver operating characteristic (ROC) curves yielded areas under the curve (AUCs) of 0.682 and 0.758 for clinical only or combined CT features, respectively, with a significant difference (p < 0.001). Furthermore, the exon 21 mutation rate in GGO was significantly higher than the exon 19 mutation rate(p = 0.029). The most significant independent prognostic factors of ALK positivity were age, solid-predominant-subtype tumours, mucinous lung adenocarcinoma, solid tumours and no air bronchograms on CT. ROC curve analysis showed that for predicting ALK positivity, the use of clinical variables combined with CT features (AUC = 0.739) was superior to the use of clinical variables alone (AUC = 0.657), with a significant difference (p = 0.0082). The use of CT features for patients may allow analyses of tumours and more accurately predict patient populations who will benefit from therapies targeting treatment.
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http://dx.doi.org/10.1038/s41598-021-83646-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952563PMC
March 2021

Actinokineospora xionganensis sp. nov., a filamentous actinomycete isolated from the lakeside soil of Baiyangdian.

Antonie Van Leeuwenhoek 2021 May 6;114(5):487-496. Epub 2021 Mar 6.

College of Life Science, Institute of Life Science and Green Development, Hebei University; Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province; Key Laboratory of Microbial Diversity Research and Application of Hebei Province; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, 071002, PR China.

A novel actinomycete, strain HBU206404, belonging to the genus Actinokineospora, was isolated from the lakeside soil of Baiyangdian, in China. Cells grew at 9-37 °C (optimum temperature, 28 °C) and pH 6-9 (optimum, pH 7). Meso-diaminopimelic acid was the diagnostic diamino acid of the cell-wall peptidoglycan, and sugars present in whole-cell hydrolysates were arabinose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H). The predominant cellular fatty acids (> 5%) of the strain HBU206404 were iso-C (21.5%), iso-C (20.3%), Cω7c/Cω6c and/or Cω6c/Cω7c (15.0%), iso-C (8.6%), C (7.0%) and Cω8c (6.9%). The major polar lipids of the strain HBU206404 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, six unknown aminolipids, two unknown aminolipids and an unidentified lipid. The 16S rRNA gene sequence analysis indicated that the strain HBU206404 was most closely related to Actinokineospora alba KCTC 19294 (99.58%), but whole-genome comparisons, using average nucleotide identity (ANI) value (91.77%) and digital DNA-DNA hybridization (dDDH) value (60%), confirmed low genome relatedness. Nitrogen metabolism pathway was found in the genome of strain HBU206404 which haboured nitrate reductase and nitrite reductase. Other phenotypic characteristics, such as ability to hydrolyze substances, enzyme activity, acid production from carbon source, etc., could also distinguish strain HBU206404 from Actinokineospora alba KCTC 19294. On the basis of genetic and phenotypic evidence, strain HBU206404 represents a novel species of the genus Actinokineospora, for which the name Actinokineospora xionganensis sp. nov. is proposed. The type strain is HBU206404 (= MCCC 1K04412 = KCTC 49404).
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http://dx.doi.org/10.1007/s10482-021-01532-8DOI Listing
May 2021
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