Publications by authors named "Yuhua He"

25 Publications

  • Page 1 of 1

Pelleting of a Total Mixed Ration Affects Growth Performance of Fattening Lambs.

Front Vet Sci 2021 12;8:629016. Epub 2021 Feb 12.

The Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin City, China.

Feeding pelleted total mixed rations (TMR) instead of traditional loose concentrate plus forage to fattening lambs is an emerging practice. This study aimed to determine the effects of feeding pelleted TMR to fattening lambs on feed intake behaviour, growth performance, feed digestion, rumen fermentation characteristics, rumen microbial community, serum parameters, slaughter performance, meat quality, and the economic outcome. Two physical forms (pelleted vs. un-pelleted) of TMR composed of the same ingredients with the same particle sizes were compared in three animal experiments. Feed intake and average daily gain were higher when the TMR was pelleted, but apparent total tract digestibility of nutrients (organic matter, crude protein, neutral detergent fibre, acid detergent fibre, and ether extract) and serum parameters were not affected and apparent total tract dry matter digestibility was slightly lower. Feeding pelleted TMR increased total short-chain fatty acid concentration and decreased rumen pH. Rumen microbial community was not affected by the physical form of the TMR at phylum level but changed slightly at genus level. Liveweight at slaughter and hot carcass weight were higher for lambs fed the pelleted compared to the un-pelleted TMR, while dressing percentage and meat quality were not affected. In conclusion, feeding pelleted TMR improves growth performance of fattening lambs mainly due to an increase in feed intake. Feeding pelleted TMR is a feasible strategy for intensive lamb fattening operations.
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http://dx.doi.org/10.3389/fvets.2021.629016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928353PMC
February 2021

QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps.

BMC Plant Biol 2021 Mar 3;21(1):126. Epub 2021 Mar 3.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, China.

Background: Melon is a very important horticultural crop produced worldwide with high phenotypic diversity. Fruit size is among the most important domestication and differentiation traits in melon. The molecular mechanisms of fruit size in melon are largely unknown.

Results: Two high-density genetic maps were constructed by whole-genome resequencing with two F segregating populations (WAP and MAP) derived from two crosses (cultivated agrestis × wild agrestis and cultivated melo × cultivated agrestis). We obtained 1,871,671 and 1,976,589 high quality SNPs that show differences between parents in WAP and MAP. A total of 5138 and 5839 recombination events generated 954 bins in WAP and 1027 bins in MAP with the average size of 321.3 Kb and 301.4 Kb respectively. All bins were mapped onto 12 linkage groups in WAP and MAP. The total lengths of two linkage maps were 904.4 cM (WAP) and 874.5 cM (MAP), covering 86.6% and 87.4% of the melon genome. Two loci for fruit size were identified on chromosome 11 in WAP and chromosome 5 in MAP, respectively. An auxin response factor and a YABBY transcription factor were inferred to be the candidate genes for both loci.

Conclusion: The high-resolution genetic maps and QTLs analyses for fruit size described here will provide a better understanding the genetic basis of domestication and differentiation, and provide a valuable tool for map-based cloning and molecular marker assisted breeding.
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http://dx.doi.org/10.1186/s12870-021-02904-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931605PMC
March 2021

Characterization of a Novel Bacteriophage Henu2 and Evaluation of the Synergistic Antibacterial Activity of Phage-Antibiotics.

Antibiotics (Basel) 2021 Feb 9;10(2). Epub 2021 Feb 9.

Institute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, China.

phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43513bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage Henu2 belongs to the family and possesses an isometric head (63 nm in diameter). The latent time and burst size of Henu2 were approximately 20 min and 7.8 plaque forming unit (PFU)/infected cells. The Henu2 maintained infectivity over a wide range of temperature (10-60 °C) and pH values (4-12). Phylogenetic and comparative genomic analyses indicate that phage Henu2 should be a new member of the family of class-II. In this paper, Phage Henu2 alone exhibited weak inhibitory activity on the growth of . However, the combination of phage Henu2 and some antibiotics or oxides could effectively inhibit the growth of , with a decrease of more than three logs within 24 h in vitro. These results provide useful information that phage Henu2 can be combined with antibiotics to increase the production of phage Henu2 and thus enhance the efficacy of bacterial killing.
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http://dx.doi.org/10.3390/antibiotics10020174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916345PMC
February 2021

Genetic dissection of climacteric fruit ripening in a melon population segregating for ripening behavior.

Hortic Res 2020 Nov 1;7(1):187. Epub 2020 Nov 1.

Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Edifici CRAG, Campus UAB, 08193, Cerdanyola, Barcelona, Spain.

Melon is as an alternative model to understand fruit ripening due to the coexistence of climacteric and non-climacteric varieties within the same species, allowing the study of the processes that regulate this complex trait with genetic approaches. We phenotyped a population of recombinant inbred lines (RILs), obtained by crossing a climacteric (Védrantais, cantalupensis type) and a non-climcteric variety (Piel de Sapo T111, inodorus type), for traits related to climacteric maturation and ethylene production. Individuals in the RIL population exhibited various combinations of phenotypes that differed in the amount of ethylene produced, the early onset of ethylene production, and other phenotypes associated with ripening. We characterized a major QTL on chromosome 8, ETHQV8.1, which is sufficient to activate climacteric ripening, and other minor QTLs that may modulate the climacteric response. The ETHQV8.1 allele was validated by using two reciprocal introgression line populations generated by crossing Védrantais and Piel de Sapo and analyzing the ETHQV8.1 region in each of the genetic backgrounds. A Genome-wide association study (GWAS) using 211 accessions of the ssp. melo further identified two regions on chromosome 8 associated with the production of aromas, one of these regions overlapping with the 154.1 kb interval containing ETHQV8.1. The ETHQV8.1 region contains several candidate genes that may be related to fruit ripening. This work sheds light into the regulation mechanisms of a complex trait such as fruit ripening.
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http://dx.doi.org/10.1038/s41438-020-00411-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603510PMC
November 2020

Shenshuaikang Enema, a Chinese Herbal Remedy, Inhibited Hypoxia and Reoxygenation-Induced Apoptosis in Renal Tubular Epithelial Cells by Inhibiting Oxidative Damage-Dependent JNK/Caspase-3 Signaling Pathways Using Network Pharmacology.

Evid Based Complement Alternat Med 2020 17;2020:9457101. Epub 2020 Nov 17.

Department of Clinical Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Background: Acute kidney injury (AKI) is a common clinically critical illness with serious consequences for the patients. Shenshuaikang enema (SE) is a Chinese herbal compound that is used to treat AKI in clinical practice. However, its mechanism of action remains unclear.

Aim: The aim of this study was to investigate the therapeutic effect of SE and explore the molecular mechanisms using network pharmacology and in vitro experiments.

Materials And Methods: The herb-component-target network was constructed based on network pharmacology. The predicted targets and pathways were validated using in vitro experiments. A renal tubular epithelial cell line (HK-2 cells) was exposed to hypoxia and reoxygenation (H/R) using air-tight conditions for five hours and treated with different concentrations of SE (25%, 50%, and 75%) to assess cell viability and apoptosis and determine the optimal experimental dose. Subsequently, H/R-injured HK-2 cells were pretreated with the optimal SE dose and then randomly divided into three groups, the SE, SE-SP600125 (inhibitor of JNK), and SE-NAC (antioxidant) groups. The cell vitality, apoptosis, and death were evaluated using the cell counting kit 8 (CCK8) and carboxyfluorescein succinimidyl ester/propidium iodide (CFSF/PI) staining. The apoptosis-related protein JNK and Caspase-3 were assessed by Western blot. Expression of JNK and Caspase-3 genes was analyzed using real-time quantitative polymerase chain reaction (RT-qPCR).

Results: 123 active components and 226 targets were identified from four herbs that composed the herb-compound-target network based on transcriptomics and network pharmacology analyses. The KEGG pathway analyses revealed that the mitochondrial apoptosis pathway was involved in the therapeutic AKI effects of SE. Cell vitality of H/R-induced HK-2 cells was obviously increased when treating them with SE, and the apoptosis was significantly inhibited, especially in the SE (50%) group at 4 and 12 h after modeling. Pretreatment with antioxidant NAC obviously prevented cell death compared to the SE (50%) group, while no obvious reduction of apoptosis was observed in the SP600125 group. JNK expression level was significantly increased in the SE (50%) group compared to the SP600125 ( < 0.01) and the NAC group ( < 0.05). Caspase-3 was downregulated in the SE (50%) group compared to the SP600125 ( < 0.01) and NAC group ( < 0.05). Caspase-3 activation in the SP600125 group was higher than that in the NAC group ( < 0.05). Moreover, the oxidative damage-dependent JNK/Caspase-3 pathway was identified in the H/R-injured HK-2 cells by inhibiting the JNK activation and oxidative damage.

Conclusions: Our findings suggested that the H/R-triggered apoptosis in HK-2 cells was abrogated by SE by upregulating the oxidative damage-dependent JNK to trigger suppression of Caspase-3.
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http://dx.doi.org/10.1155/2020/9457101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685836PMC
November 2020

Genetic mapping and candidate gene analysis for melon resistance to Phytophthora capsici.

Sci Rep 2020 11 24;10(1):20456. Epub 2020 Nov 24.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, Henan, China.

Phytophthora blight is one of the most serious diseases affecting melon (Cucumis melo) production. Due to the lack of highly resistant germplasms, the progress on disease-resistant research is slow. To understand the genetics of melon resistance to Phytophthora capsici, an F population containing 498 individuals was developed by crossing susceptible line E31 to highly resistant line ZQK9. Genetic analysis indicated that the resistance in ZQK9 was controlled by a dominant gene, tentatively named MePhyto. Through bulked-segregant analysis (BSA-Seq) and chromosome walking techniques, the MePhyto gene was mapped to a 52.44 kb interval on chromosome 12. In this region, there were eight genes and their expression patterns were validated by qRT-PCR. Among them, one wall-associated receptor kinase (WAK) gene MELO3C002430 was significantly induced in ZQK9 after P. capsici inoculation, but not in E31. Based on the non-synonymous mutation site in MELO3C002430, a cleaved amplified polymorphic sequence (CAPS) marker, CAPS2430, was developed and this maker was co-segregated with MePhyto in both F population and a collection of 36 melon accessions. Thus MELO3C002430 was considered as the candidate gene and CAPS2430 was a promising marker for marker-assisted selection (MAS) in breeding. These results lay a foundation for revealing the resistance mechanism of melon to P. capsici.
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http://dx.doi.org/10.1038/s41598-020-77600-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686303PMC
November 2020

Animal, feed and rumen fermentation attributes associated with methane emissions from sheep fed brassica crops.

J Anim Physiol Anim Nutr (Berl) 2021 Mar 6;105(2):210-218. Epub 2020 Oct 6.

Jilin Inter-regional Cooperation Centre for the Scientific and Technological Innovation of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin, China.

Methane emissions from ruminants enhance global warming and lead to a loss of feed energy. The emissions are low when fed brassica crops, but the factors contributing to low emissions are unknown. A meta-analysis was conducted with individual animal data collected from seven experiments. In these experiments, methane emissions were measured using respiration chambers. Animal characteristics, feed chemical composition and rumen fermentation parameters were included for the analysis using multiple regression models. Feed intake level, animal live weight and age were animal factors that were weakly and negatively related to methane yield (g/dry matter intake). The duration in which sheep were fed brassica crops was a significant contributor in the model, suggesting that the effect on emissions diminishes with time. Among a range of feed chemical composition characters, acid detergent fibre and hot-water-soluble carbohydrate contributed significantly to the model, suggesting that both structural and soluble carbohydrates affect methane formation in the rumen. There was no significant correlation between the concentration of sulphate in brassicas and emissions, but nitrate was moderately and negatively correlated with methane yield (r = -.53). Short-chain fatty acid profiles in the rumen of animals fed brassicas were different from those fed pasture, but these parameters only moderately correlated to methane emissions (r = .42). Feeding forage rape resulted in low rumen pH. The pH before morning feeding was strongly correlated to methane yield (r = .90). Rumen pH, together with microbial communities mediated by pH, might lead to low emissions. Bacteria known to produce hydrogen were relatively less abundant in the rumen contents of brassica-fed animals than pasture-fed animals. In conclusion, animal and feed factors, rumen fermentation and microbial communities all affect methane emissions to some extent. The interactions of these factors with each other thus contribute to methane emissions from brassica-fed sheep.
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http://dx.doi.org/10.1111/jpn.13460DOI Listing
March 2021

Biochemical and structural characterization of the Holliday junction resolvase RuvC from Pseudomonas aeruginosa.

Biochem Biophys Res Commun 2020 04 19;525(2):265-271. Epub 2020 Feb 19.

College of Chemistry, Fuzhou University, Fuzhou, 350108, China. Electronic address:

The Holliday junction, a four-way DNA structure, is an important intermediate of homologous recombination. Proper Holliday junction resolution is critical to complete the recombination process. In most bacterial cells, the Holliday junction cleavage is mainly performed by a specific endonuclease RuvC. Here, we describe the biochemical properties and the crystal structure of RuvC from an opportunistic pathogen, Pseudomonas aeruginosa (PaRuvC). PaRuvC specifically binds to the Holliday junction DNA and preferentially cleaves it at the consensus 5'-TTC-3'. PaRuvC uses Mg as the preferred divalent metal cofactor for Holliday junction cleavage and its optimum pH is 8.0-9.0. Elevated temperatures (37-60 °C) boost the catalytic activity, but temperatures higher than 53 °C reduce the protein stability. The crystal structure of PaRuvC determined at 2.4 Å and mutagenesis analysis reveal key residues involved in the dimer formation, substrate binding and catalysis. Our results are expected to provide useful information to combat antibiotic resistance of Pseudomonas aeruginosa by targeting its homologous recombination system.
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http://dx.doi.org/10.1016/j.bbrc.2020.02.062DOI Listing
April 2020

Colorectal patients' readiness for hospital discharge following management of enhanced recovery after surgery pathway: A cross-sectional study from China.

Medicine (Baltimore) 2020 Feb;99(8):e19219

West China School of Nursing, Sichuan University, Chengdu 610041, PR China.

The aim of this study was to ascertain the status quo of perceived readiness for hospital discharge in colorectal cancer patients who underwent enhanced recovery pathway and identify the variables that affect patients' perceptions about their readiness for discharge.A cross-sectional survey was conducted in West China Hospital, Sichuan University. The Readiness for Hospital Discharge Scale and the Quality of Discharge Teaching Scale were delivered to 130 colorectal cancer (CRC) patients who underwent enhanced recovery pathway. Data collection was carried out 4 hours before discharge.The total score of readiness for hospital discharge was 149.86 ± 33.65. The multiple linear regression analysis revealed that the quality of discharge teaching, discharge to a rehabilitative institution were associated with the readiness for hospital discharge.The level of CRC patients' readiness for hospital discharge needs to be improved. Medical staff should improve the quality of discharge guidance and pay more attention to patients transferred to rehabilitation institutions when they leave hospital.
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http://dx.doi.org/10.1097/MD.0000000000019219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034666PMC
February 2020

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon.

PLoS One 2020 12;15(2):e0227284. Epub 2020 Feb 12.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan Province, China.

Phytophthora blight caused by Phytophthora capsici is a devastating disease for melon plant. However, the underlying resistance mechanisms are still poorly understood. In this study, the transcriptome differences between the resistant ZQK9 and susceptible E31 at 0, 3, and 5 days post-inoculation (dpi) were identified by RNA-seq. A total of 1,195 and 6,595 differentially expressed genes (DEGs) were identified in ZQK9 and E31, respectively. P. capsici infection triggered massive transcript changes in the inoculated tissues. Genes related to plant defense responses were activated, which was reflected by a lot of up-regulated DEGs involved in pathogenesis-related (PR) genes, hormones biosynthesis and signal transduction, secondary metabolites biosynthesis and cell wall modification in resistant ZQK9. The dataset generated in this study may provide a basis for identifying candidate resistant genes in melon against P. capsici and lay a foundation for further research on the molecular mechanisms.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227284PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015699PMC
May 2020

Effects of the Supplementation of Lysophospholipids through Pelleted Total Mixed Rations on Blood Biochemical Parameters and Milk Production and Composition of Mid-Lactation Dairy Cows.

Animals (Basel) 2020 Jan 28;10(2). Epub 2020 Jan 28.

The Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin 132109, China.

Lysophospholipids (LPL), a new feed additive, were supplemented to a pelleted total mixed ration (TMR) of dairy cows to examine its effects on feed intake, production, and composition of milk and plasma biochemical parameters. Two dietary treatments included diets supplemented without (control diet; CON) or with LPL at a dose of 0.5 g/kg of pelleted TMR. Twelve multiparous, mid-lactation, Holstein cows (Bodyweight 730 ± 9.3 kg; 100 ± 6.0 days in milk) were randomly assigned to one of the two dietary treatments with a 42-day measurement period after a 14-day adaptation period. Feed and water were provided . Feed intake and milk yields were recorded daily, blood samples were collected fortnightly, and milk samples weekly. The results showed that the supplementation of LPL did not change feed dry matter intake, milk yields, and milk composition. However, it increased total protein and globulin and the activity of alkaline phosphatase and decreased total cholesterol in plasma. This study suggests that LPL may have beneficent effects in animal health but might be not a feasible feed additive to increase production for dairy cows fed a pelleted TMR.
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http://dx.doi.org/10.3390/ani10020215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071077PMC
January 2020

Is there a cardiovascular protective effect of aspirin in chronic kidney disease patients? A systematic review and meta-analysis.

Int Urol Nephrol 2020 Feb 9;52(2):315-324. Epub 2019 Dec 9.

No. 1 Department of Nephrology, Hospital of Chengdu University of Traditional Chinese Medicine, No. 37 Shierqiao Road, Chengdu, Sichuan Province, People's Republic of China.

Purpose: To perform a systematic review and meta-analysis to evaluate the cardiovascular prevention effect of aspirin among patients with chronic kidney disease (CKD).

Methods: A comprehensive literature search was conducted in Embase, PubMed, and Cochrane library (up to March 2019) without language limitations. Randomized control trials (RCT) and observational studies that met the inclusion and exclusion criteria were included. Two reviewers independently extracted data, and evaluated study quality using modified Jadad score for RCTs and Newcastle-Ottawa Scale for observational study. A meta-analysis was conducted in the Stata 15.0 software using the DerSimonian and Laird random-effects model.

Results: 1768 references were identified from literature searching. Four RCTs and four cohort studies that reported the cardiovascular prevention outcome of aspirin in CKD patients (38,341 participants) were included in this review. The pooled data revealed that aspirin had no significant prevention effect on cardiovascular events among CKD patients (RR = 0.96, 95% CI, 0.59-1.13). There was also no significant reduction in cardiovascular mortality and all-cause mortality. Although we found no significant increased risk in major bleeding events, there was a statistically significant increased risk of minor bleeding events (RR = 2.57, 95% CI, 1.60-4.13) and renal events (RR = 1.30, 95% CI, 1.02-1.65) for aspirin use.

Conclusion: Our review indicated that aspirin use in CKD patients had no prevention effect on cardiovascular events and no statistically significant reduction in risk of cardiovascular or all-cause mortality, with a significant increased risk of minor bleeding and renal events.
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http://dx.doi.org/10.1007/s11255-019-02350-8DOI Listing
February 2020

A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits.

Nat Genet 2019 11 1;51(11):1607-1615. Epub 2019 Nov 1.

Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Melon is an economically important fruit crop that has been cultivated for thousands of years; however, the genetic basis and history of its domestication still remain largely unknown. Here we report a comprehensive map of the genomic variation in melon derived from the resequencing of 1,175 accessions, which represent the global diversity of the species. Our results suggest that three independent domestication events occurred in melon, two in India and one in Africa. We detected two independent sets of domestication sweeps, resulting in diverse characteristics of the two subspecies melo and agrestis during melon breeding. Genome-wide association studies for 16 agronomic traits identified 208 loci significantly associated with fruit mass, quality and morphological characters. This study sheds light on the domestication history of melon and provides a valuable resource for genomics-assisted breeding of this important crop.
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http://dx.doi.org/10.1038/s41588-019-0522-8DOI Listing
November 2019

Dietary Supplementation of Lysophospholipids Affects Feed Digestion in Lambs.

Animals (Basel) 2019 Oct 15;9(10). Epub 2019 Oct 15.

The Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin 132109, China.

Five experiments were conducted to examine effects of lysophospholipids (LPL) on live weight gain, nutrient digestibility, ruminal fermentation parameters, serum biochemical parameters and rumen bacterial community profile in fattening lambs. Two dietary treatments (pelleted complete feed supplemented without (control diet; CON) or with 0.05% LPL on dry matter basis) were tested in these experiments. Feed and water were provided to lambs. The results showed that average daily gain (ADG) tended to increase or was not affected by LPL supplementation. Compared with CON, the supplementation of LPL resulted in an increase in dry matter, crude protein and organic matter digestibilities, and a decrease in neutral detergent fiber and acid detergent fiber digestibilities. Ruminal pH values did not change with LPL supplementation, but the concentrations of ammonia and total short chain fatty acids (SCFAs) were increased. The molar proportion of major individual SCFAs and the ratio of acetate to propionate were not affected by LPL supplementation. While the activity of lipase was decreased with LPL supplementation, all other serum biochemical parameters did not change. Rumen bacterial community was altered by LPL supplementation with the relative abundance of fibrolytic bacteria in the total bacterial population, such as , decreased. In conclusion, LPL supplementation can alter feed digestion, but may not result in consistent positive responses in animal growth performance.
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http://dx.doi.org/10.3390/ani9100805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826496PMC
October 2019

Two Novel Alleles Conferring Powdery Mildew () Resistance Identified in a Worldwide Collection of Pea ( L.) Germplasms.

Int J Mol Sci 2019 Oct 12;20(20). Epub 2019 Oct 12.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Powdery mildew caused by DC. severely affects pea crops worldwide. The use of resistant cultivars containing the gene is the most effective way to control this disease. The objectives of this study were to reveal alleles contained in 55 -resistant pea germplasms and to develop the functional markers of novel alleles. Sequences of 10 homologous cDNA clones from each germplasm accession were used to determine their alleles. The frame shift mutations and various alternative splicing patterns were observed during transcription of the gene. Two novel alleles, -8 and -9, were discovered in the germplasm accessions G0004839 and G0004400, respectively, and four known alleles were identified in 53 other accessions. One mutation in G0004839 was characterized by a 3-bp (GTG) deletion of the wild-type cDNA, resulting in a missing valine at position 447 of the PsMLO1 protein sequence. Another mutation in G0004400 was caused by a 1-bp (T) deletion of the wild-type cDNA sequence, resulting in a serine to leucine change of the PsMLO1 protein sequence. The -8 and -9 alleles were verified using resistance inheritance analysis and genetic mapping with respectively derived F and F populations. Finally, co-dominant functional markers specific to -8 and -9 were developed and validated in populations and pea germplasms. These results improve our understanding of resistance in pea germplasms worldwide and provide powerful tools for marker-assisted selection in pea breeding.
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http://dx.doi.org/10.3390/ijms20205071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829425PMC
October 2019

Development and Validity of a Questionnaire on Dyslipidemia Dietary Knowledge.

Biomed Res Int 2019 2;2019:5382130. Epub 2019 Jun 2.

School of Nursing, Qingdao University, 38 Dengzhou Road, Qingdao, Shandong province 266021, China.

Objective: Dyslipidemia is one of the most important modifiable risk factors for cardiovascular disease. Diet and lifestyle interventions positively contribute to the management of dyslipidemia. Adequate knowledge of the correct diet for dyslipidemia is an effective preventive strategy against cardiovascular disease.

Method: This study developed a questionnaire to test dyslipidemia dietary knowledge. The initial, multiple-choice dyslipidemia dietary knowledge questionnaire (DDKQ) was formed based on a literature review of previous studies. Two Delphi rounds were performed to ensure content validity and face validity, items were pretested and filtered through item analysis, and the reliability and validity of the final questionnaire were checked.

Results: The DDKQ consisted of 5 parts, with 25 items in total. It demonstrated adequate content validity (0.94), face validity, internal consistency (KR20 = 0.67), and test-retest reliability (0.74). The mean item difficulty (P) was 0.55 and ranged from 0.15 to 0.83, and the mean item discrimination index (D) was 0.36, ranging from 0.21 to 0.58. The questionnaire was also able to distinguish between participants with/without a medical background; medical workers produced significantly higher total scores (16.70±2.84 vs. 14.57±4.26, p< 0.05).

Conclusion: The DDKQ is a reliable and valid measure of dyslipidemia dietary knowledge. It is suitable for providing scientific assessments for targeted health education interventions.
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http://dx.doi.org/10.1155/2019/5382130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582826PMC
January 2020

Antioxidant Structure⁻Activity Relationship Analysis of Five Dihydrochalcones.

Molecules 2018 05 12;23(5). Epub 2018 May 12.

School of Basic Medical Science, Guangzhou University of Chinese Medicine, Waihuan East Road No. 232, Guangzhou Higher Education Mega Center, Guangzhou 510006, China.

The study determined the comparative antioxidant capacities of five similar dihydrochalcones: phloretin, phloridzin, trilobatin, neohesperidin dihydrochalcone, and naringin dihydrochalcone. In the ferric-reducing antioxidant power (FRAP) assay, the antioxidant activities of pairs of dihydrochalcones had the following relationship: phloretin > phloridzin, phloretin > trilobatin, trilobatin > phloridzin, trilobatin > naringin dihydrochalcone, and neohesperidin dihydrochalcone > naringin dihydrochalcone. Similar relative antioxidant levels were also obtained from 1,1-diphenyl-2-picryl-hydrazl radical (DPPH•)-scavenging, 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS•⁺)-scavenging, and superoxide radical (•O₂)-scavenging assays. Using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC−ESI−Q−TOF−MS/MS) analysis for the reaction products with DPPH•, phloretin, phloridzin, and trilobatin were found to yield both dihydrochalcone-DPPH adduct and dihydrochalcone-dihydrochalcone dimer, whereas naringin dihydrochalcone gave a naringin dihydrochalcone-DPPH adduct, and neohesperidin dihydrochalcone gave a dimer. In conclusion, the five dihydrochalcones may undergo redox-based reactions (especially electron transfer (ET) and hydrogen atom transfer (HAT)), as well as radical adduct formation, to exert their antioxidant action. Methoxylation at the -OH enhances the ET and HAT potential possibly via p-π conjugation, whereas the glycosylation of the ⁻OH group not only reduces the ET and HAT potential but also hinders the ability of radical adduct formation. The 2′,6′-di-OH moiety in dihydrochalcone possesses higher ET and HAT activities than the 2′,4′-di-OH moiety because of its resonance with the adjacent keto group.
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http://dx.doi.org/10.3390/molecules23051162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100071PMC
May 2018

Short- and long-term outcomes of laparoscopic radical hysterectomy for obese patients with cervical cancer.

J BUON 2017 Jul-Aug;22(4):958-965

Department of Obstetrics and Gynecology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, People's Republic of China.

Purpose: To perform a preliminary comparison of the short- and long-term outcomes of laparoscopic hysterectomy (LH) between obese and non-obese patients with cervical cancer.

Methods: A total of 243 cases of cervical cancer patients were treated with LH between April 2009 and January 2016. Based on their body mass index (BMI) at the time of surgery, patients were divided into an obese group (BMI ≥ 25 kg/m) and a non-obese group (BMI < 25 kg/m). The short- and long-term outcomes of the two groups were retrospectively analyzed.

Results: Sixty-four patients were classified as obese and 179 as non-obese. There were no statistically significant differences in age, clinical stage, pathological type, pathological grade, and American Society of Anesthesiologists (ASA) score between the two groups. The proportion of patients with type-2 diabetes mellitus (p=0.006), hypertension (p=0.021), and hyperlipidemia (p=0.008) in the obese group was significantly higher than that in the non-obese group. Compared with the non-obese group, the obese patients had longer operative time (p=0.039), more intraoperative blood loss (p=0.025), and a higher rate of conversion (p=0.025). There was no significant difference between the two groups in terms of intraoperative and postoperative 30-day complications. Pathology data of the two groups were similar. Both groups had similar tumor recurrence rates, 5-year overall survival rates, and 5-year disease-free survival rates.
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July 2019

Comparative transcriptome analysis of Brucella melitensis in an acidic environment: Identification of the two-component response regulator involved in the acid resistance and virulence of Brucella.

Microb Pathog 2016 Feb 12;91:92-8. Epub 2015 Dec 12.

Jilin Agricultural Science and Technology University, Jilin 132101, China.

Brucella melitensis, encounters a very stressful environment in phagosomes, especially low pH levels. So identifying the genes that contribute to the replication and survival within an acidic environment is critical in understanding the pathogenesis of the Brucella bacteria. In our research, comparative transcriptome with RNA-seq were used to analyze the changes of genes in normal-medium culture and in pH4.4-medium culture. The results reveal that 113 genes expressed with significant differences (|log2Ratio| ≥ 3); about 44% genes expressed as up-regulated. With GO term analysis, structural constituent of the ribosome, rRNA binding, structural molecule activity, and cation-transporting ATPase activity were significantly enriched (p-value ≤ 0.05). These genes distributed in 51 pathways, in which ribosome and photosynthesis pathways were significantly enriched. Six pathways (oxidative phosphorylation, iron-transporting, bacterial secretion system, transcriptional regulation, two-component system, and ABC transporters pathways) tightly related to the intracellular survival and virulence of Brucella were analyzed. A two-component response regulator gene in the transcriptional regulation pathway, identified through gene deletion and complementary technologies, played an important role in the resistance to the acid-resistance and virulence of Brucella.
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http://dx.doi.org/10.1016/j.micpath.2015.11.007DOI Listing
February 2016

High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety.

PLoS One 2015 6;10(10):e0139775. Epub 2015 Oct 6.

The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139775PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595016PMC
June 2016

Graphene Quantum Dots Supported by Graphene Nanoribbons with Ultrahigh Electrocatalytic Performance for Oxygen Reduction.

J Am Chem Soc 2015 Jun 15;137(24):7588-91. Epub 2015 Jun 15.

†College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang 325035, China.

A new class of oxygen reduction reaction (ORR) catalysts based on graphene quantum dots (GQDs) supported by graphene nanoribbons (GNRs) has been developed through a one-step simultaneous reduction reaction, leading to ultrahigh performance for O reduction with an excellent electrocatalytic activity (higher limiting current density and lower overpotential than those of platinum) and high selectivity and stability in alkaline media comparable to the best C-based ORR catalysts reported so far. Electron microscopy revealed numerous surface/edge defects on the GQD/GNR surfaces and at their interface to act as the active sites. This, coupled with efficient charge transfer between the intimately contacted GQDs and GNRs, rationalized the observed ultrahigh electrocatalytic performance for the resultant GQD-GNR hybrids. Thus, this study opens a new direction for developing low-cost, highly efficient, C-based ORR electrocatalysts.
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http://dx.doi.org/10.1021/jacs.5b03799DOI Listing
June 2015

The selective formation of graphene ranging from two-dimensional sheets to three-dimensional mesoporous nanospheres.

Nanoscale 2014 Jul;6(13):7204-8

Nano-materials and Chemistry Key Laboratory, Wenzhou University, Wenzhou, Zhejiang, China 325027.

This research presents a template-free solvothermal method which offers selective preparation of graphene ranging from two-dimensional sheets to 3-dimensional nanospheres. The thus prepared nanospheres have size-defined mesopores with a huge surface area and, after doping with nitrogen, exhibited stronger electrocatalytic activity toward oxygen reduction than commercial Pt/C catalysts.
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http://dx.doi.org/10.1039/c4nr00935eDOI Listing
July 2014

Single-copy gene-based chromosome painting in cucumber and its application for chromosome rearrangement analysis in Cucumis.

Plant J 2014 Apr 12;78(1):169-79. Epub 2014 Mar 12.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Chromosome painting based on fluorescence in situ hybridization (FISH) has played an important role in chromosome identification and research into chromosome rearrangements, diagnosis of chromosome abnormalities and evolution in human and animal species. However, it has not been applied widely in plants due to the large amounts of dispersed repetitive sequences in chromosomes. In the present work, a chromosome painting method for single-copy gene pools in Cucumis sativus was successfully developed. Gene probes with sizes above 2 kb were detected consistently. A cucumber karyotype was constructed based on FISH using a cocktail containing chromosome-specific gene probes. This single-copy gene-based chromosome painting (ScgCP) technique was performed by PCR amplification, purification, pooling, labeling and hybridization onto chromosome spreads. Gene pools containing sequential genes with an interval less than 300 kb yielded painting patterns on pachytene chromosomes. Seven gene pools corresponding to individual chromosomes unambiguously painted each chromosome pair of C. sativus. Three mis-aligned regions on chromosome 4 were identified by the painting patterns. A probe pool comprising 133 genes covering the 8 Mb distal end of chromosome 4 was used to evaluate the potential utility of the ScgCP technique for chromosome rearrangement research through cross-species FISH in the Cucumis genus. Distinct painting patterns of this region were observed in C. sativus, C. melo and C. metuliferus species. A comparative chromosome map of this region was constructed between cucumber and melon. With increasing sequence resources, this ScgCP technique may be applied on any other sequenced species for chromosome painting research.
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http://dx.doi.org/10.1111/tpj.12453DOI Listing
April 2014

Integration of high-resolution physical and genetic map reveals differential recombination frequency between chromosomes and the genome assembling quality in cucumber.

PLoS One 2013 6;8(5):e62676. Epub 2013 May 6.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

Cucumber is an important model crop and the first species sequenced in Cucurbitaceae family. Compared to the fast increasing genetic and genomics resources, the molecular cytogenetic researches in cucumber are still very limited, which results in directly the shortage of relation between plenty of physical sequences or genetic data and chromosome structure. We mapped twenty-three fosmids anchored by SSR markers from LG-3, the longest linkage group, and LG-4, the shortest linkage group on pachytene chromosomes 3 and 4, using uorescence in situ hybridization (FISH). Integrated molecular cytogenetic maps of chromosomes 3 and 4 were constructed. Except for three SSR markers located on heterochromatin region, the cytological order of markers was concordant with those on the linkage maps. Distinct structural differences between chromosomes 3 and 4 were revealed by the high resolution pachytene chromosomes. The extreme difference of genetic length between LG-3 and LG-4 was mainly attributed to the difference of overall recombination frequency. The significant differentiation of heterochromatin contents in chromosomes 3 and 4 might have a direct correlation with recombination frequency. Meanwhile, the uneven distribution of recombination frequency along chromosome 4 was observed, and recombination frequency of the long arm was nearly 3.5 times higher than that of the short arm. The severe suppression of recombination was exhibited in centromeric and heterochromatin domains of chromosome 4. Whereas a close correlation between the gene density and recombination frequency was observed in chromosome 4, no significant correlation was observed between them along chromosome 3. The comparison between cytogenetic and sequence maps revealed a large gap on the pericentromeric heterochromatin region of sequence map of chromosome 4. These results showed that integrated molecular cytogenetic maps can provide important information for the study of genetic and genomics in cucumber.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062676PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646037PMC
December 2013

[Determination and status quo survey of phathalic acid esters in air of plastic production logistics by GC-MS].

Wei Sheng Yan Jiu 2010 Jan;39(1):45-9

School of Economics and Management, Zhejiang Business Technology Institute, Ningbo 315012, China.

Objective: To establish GC-MS method for the determination of phathalic acid esters (PAEs) in the air of plastic production logistics, and conduct a status quo survey of PAEs pollution in occupational environments basing on it.

Methods: The determination method was established according to the Monitor Investigating Guidelines on Poisons in Workplace Air, and then used to determine samples collected to carry out an on-the-spot investigation.

Results: Calibration curves of the six PAEs were proved to be all linear in the range of 2-1000 ng/ml, equivalent to 0.67-333 microg/m3 in the air considering 3 L air sample was collected. The sensitivities were high with all limits of detection lower than 1 ng/ml, i.e., the minimum detectable concentrations of the six PAEs were all lower than 0.33 microg/m3. Intra-day precision and accuracies were all good, with relative standard deviation in the range of 3.0%-7.8% and recoveries in the range of 93.1%-105.1% respectively. And inter-day precision and accuracies were 3.4%-9.9% and 93.4%-106.4% correspondingly. The eluting efficiency was 92.9%-97.9%, and the sampling efficiencies were nearly 100%. The breakthrough capacities were all larger than 1.4 mg. And samples could be stored stably at room temperature for 7 days. The selectivity of this method was good. Results of status quo survey indicated that the PAEs pollution in the air of plastic production logistics was not serious, and no obvious clinical symptoms and signs were found in those occupationally-exposed workers.

Conclusion: This method meets the demand of the Monitor Investigating Guidelines on Poisons in Workplace Air, and thus then could be applied to determine PAEs in the air of plastic production logistics.
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January 2010