Publications by authors named "Yufeng Zhou"

162 Publications

Correction: The signal pathways and treatment of cytokine storm in COVID-19.

Signal Transduct Target Ther 2021 Aug 31;6(1):326. Epub 2021 Aug 31.

Institute of Pediatrics, Children's Hospital of Fudan University, National Children's Medical Center, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1038/s41392-021-00744-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406011PMC
August 2021

Interactions between soil properties and the rhizome-root distribution in a 12-year Moso bamboo reforested region: Combining ground-penetrating radar and soil coring in the field.

Sci Total Environ 2021 Aug 3;800:149467. Epub 2021 Aug 3.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an 311300, Zhejiang, China; Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization, Zhejiang A&F University, Lin'an 311300, Zhejiang, China; Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration of Zhejiang Province, Zhejiang A&F University, Lin'an 311300, Zhejiang, China; School of Environmental and Resources Science, Zhejiang A&F University, Lin'an 311300, Zhejiang, China. Electronic address:

Moso bamboo (Phyllostachys pubescens) plays an important role in mitigating climate change and ameliorating soil degradation because of its high carbon sequestration capacity and erosion resistance. Its strong underground rhizome-root systems form the basic framework of the aboveground system of Moso bamboo forest and define the basic ecological characteristics. However, studies on the relationship between the spatial distribution of roots and soil resources have often been neglected due to methodological limitations. The objective of this study was to test the detectability of rhizomes in the field by ground-penetrating radar (GPR) and to understand the interactions between rhizome-root systems and soil characteristics. The rhizome-root system distribution was investigated using GPR; and the soil texture, soil organic carbon and soil nutrients were investigated using a soil coring method to prepare 50-cm soil profiles. A few key findings were emphasized. First, the rhizome-root system was mainly distributed over a soil depth of 0-30 cm; and the rhizomes were larger in diameter (often greater than 1.0 cm). Therefore, GPR can accurately detect rhizomes in the field, making the non-invasive and long-term estimation of rhizome biomass and monitoring of changes in rhizome dynamics possible under field conditions. Second, the spatial heterogeneity of the soil moisture content, alkaline hydrolysed nitrogen and available phosphorus had a greater effect on the rhizomes spatial distribution than did the spatial heterogeneity of other soil characteristics. The rhizomes clonal growth led to increases in soil organic carbon, which promoted the amelioration of degraded soil. Third, the results provide insights for bamboo forest management, such as the application of GPR to prevent bamboo invasion and to determine the appropriate fertilizer level for a rhizome system. More field tests are needed to validate the application of GPR to rhizome systems and enhance the detection and quantification of rhizome systems in bamboo forest ecosystems.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149467DOI Listing
August 2021

Surface Creasing-Induced Micropatterned GelMA Using Heating-Hydration Fabrication for Effective Vascularization.

Tissue Eng Regen Med 2021 Oct 13;18(5):759-773. Epub 2021 Aug 13.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

Background: Surface modification is used to modify the biomaterials for the regulation of cell culture using different approaches, such as chemical graft and mechanical treatment. However, those conventional methodologies often require precise fabrication in a high resolution involving either high cost or laborious steps to remove chemical residues that are toxic to the cells.

Methods: A novel and simple method was proposed and evaluated to rapidly generate surface ceases on the gelatin methacrylate (gelMA) surface using the heating-hydration process. Human umbilical vein endothelial cells (HUVECs) were cultured on the gelMA surface. The surface binding was characterized using the RGD (Arg-Gly-Asp) antibodies and cell adhesion pattern captured by scanning electron microscopy. The effect of the heating-hydration parameters on the creasing formation was investigated. The morphology of HUVECs cultured on such micropatterned gelMA was characterized and compared.

Results: It is found that the hydration solution, gelMA mixture, and hydration rate are the major factors that influence the cracking sizes in the range from 20 to 120 µm which resulted in capillary-like patterns on the gelMA surface. Low concentration of gelMA, high water concentration of cooling agent, and slow hydration rate result in the long creases, and heating of at least 60 min is required for complete dehydration. Strong fluorescence was around the creases with RGD-staining. Consequently, micropatterned gelMA demonstrated good biocompatibility with endothelial cells with more than 95% cell viability and continuous cell proliferation throughout 2 weeks as well as a good trace of neovascular formation. In comparison, normal gelMA surface did not exhibit RGD-fluorescent signals, and the cultured HUVECs on it were rounded with no spreading for network formation.

Conclusion: The heating-hydration approach can successfully and easily produce the micropatterned gelMA that allows rapid and effective vascularization to potentially improve the functionalities of the tissue-engineered construct.
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http://dx.doi.org/10.1007/s13770-021-00345-0DOI Listing
October 2021

Characterization and Ex Vivo evaluation of an extracorporeal high-intensity focused ultrasound (HIFU) system.

J Appl Clin Med Phys 2021 Sep 4;22(9):345-359. Epub 2021 Aug 4.

Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, WA, USA.

Background: High-intensity focused ultrasound (HIFU) has been in clinical use for a variety of solid tumors and cancers. Accurate and reliable calibration is in a great need for clinical applications. An extracorporeal clinical HIFU system applied for the investigational device exemption (IDE) to the Food and Drug Administration (FDA) so that evaluation of its characteristics, performance, and safety was required.

Methods: The acoustic pressure and power output was characterized by a fiber optic probe and a radiation force balance, respectively, with the electrical power up to 2000 W. An in situ acoustic energy was established as the clinical protocol at the electrical power up to 500 W. Temperature elevation inside the tissue sample was measured by a thermocouple array. Generated lesion volume at different in situ acoustic energies and pathological examination of the lesions was evaluated ex vivo.

Results: Acoustic pressure mapping showed the insignificant presence of side/grating lobes and pre- or post-focal peaks (≤-12 dB). Although distorted acoustic pressure waveform was found in the free field, the nonlinearity was reduced significantly after the beam propagating through tissue samples (i.e., the second harmonic of -11.8 dB at 500 W). Temperature elevation was <10°C at a distance of 10 mm away from a 20-mm target, which suggests the well-controlled HIFU energy deposition and no damage to the surrounding tissue. An acoustic energy in the range of 750-1250 J resulted in discrete lesions with an interval space of 5 mm between the treatment spots. Histology confirmed that the lesions represented a region of permanently damaged cells by heat fixation, without causing cell lysis by either cavitation or boiling.

Conclusions: Our characterization and ex vivo evaluation protocol met the IDE requirement. The in-situ acoustic energy model will be used in clinical trials to deliver almost consistent energy to the various targets.
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http://dx.doi.org/10.1002/acm2.13074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425942PMC
September 2021

Exosomal miR-101-3p and miR-423-5p inhibit medulloblastoma tumorigenesis through targeting FOXP4 and EZH2.

Cell Death Differ 2021 Jul 22. Epub 2021 Jul 22.

Institute of Pediatrics, Children's Hospital of Fudan University, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.

Exosomal microRNAs (miRNAs) have been implicated in the development and progression of a variety of tumors; however, whether they contribute to medulloblastoma (MB) tumorigenesis remains to be elucidated. To address this, we first characterized the miRNA profiles of circulating exosomes by miRNA sequencing to identify miRNAs differentially expressed between children with MB and healthy controls. Then, we conducted in vitro and in vivo functional assays with the identified miRNAs and their predicted targets. We found that, compared with healthy controls, 35 miRNAs were upregulated and 5 downregulated in exosomes isolated from the plasma of MB patients. We further found that the expression of miR-101-3p and miR-423-5p was significantly higher in plasma exosomes from MB patients than in healthy controls in an expanded cohort and these exosomal miRNAs could be delivered to tumor cells via exosomes. An in vitro functional analysis of miR-101-3p and miR-423-5p showed that treating MB cells with the corresponding mimics significantly inhibited the proliferation, colony-forming ability, migratory ability, and invasive capacity of tumor cells, and promoted cell apoptosis. Additionally, miR-101-3p and miR-423-5p were found to act as tumor suppressors by directly targeting a common gene, FOXP4, which encodes a transcription factor with a vital role in embryonic development and tumorigenesis. Moreover, miR-101-3p also targeted EZH2, a histone methyltransferase, to reinforce its tumor inhibitory effects. Using a xenograft nude mouse model of MB, we further identified that the overexpression of miR-101-3p and miR-423-5p inhibited tumorigenesis in vivo. Our findings provide novel insights into the functions of exosomal miRNAs in mediating MB progression and suggest a potential therapeutic approach for the treatment of children with MB.
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http://dx.doi.org/10.1038/s41418-021-00838-4DOI Listing
July 2021

Associations of fine particulate matter and constituents with pediatric emergency room visits for respiratory diseases in Shanghai, China.

Int J Hyg Environ Health 2021 07 13;236:113805. Epub 2021 Jul 13.

School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China. Electronic address:

Background: Although ambient fine particulate matter (PM) has been associated with adverse respiratory outcomes in children, few studies have examined PM constituents with respiratory diseases in children in China.

Objectives: To investigate the associations of short-term exposure to PM and its constituents with pediatric emergency room visits (ERVs) for respiratory diseases in Shanghai, China.

Methods: We collected daily concentrations of PM and its constituents in urban Shanghai from January 1, 2016, to December 31, 2018. Daily pediatric ERVs for four major respiratory diseases, including upper respiratory tract infection, bronchitis, pneumonia, and asthma, were obtained from 66 hospitals in Shanghai during the same period. Associations of exposure to daily PM and constituents with respiratory ERVs were estimated using the over-dispersed generalized additive models.

Result: Short-term exposure to PM and its constituents were associated with increased pediatric ERVs for respiratory diseases. Specifically, an interquartile range increase in the 3-day average PM level (31 μg/m) was associated with 1.86% (95%CI: 0.52, 3.22), 1.53% (95%CI: 0.01, 3.08), 1.90% (95%CI: 0.30, 3.52), and 2.67% (95%CI: 0.70, 4.68) increase of upper respiratory tract infection, bronchitis, pneumonia, and asthma ERVs, respectively. As for PM constituents, we found organic carbon, ammonium, nitrate, selenium, and zinc were associated with higher risk of respiratory ERVs in the single constituent and the constituent-PM models.

Conclusion: Short-term exposure to PM was associated with increased pediatric ERVs for respiratory diseases. Constituents related to anthropogenic combustion and traffic might be the dominant contributors of the observed associations.
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http://dx.doi.org/10.1016/j.ijheh.2021.113805DOI Listing
July 2021

The signal pathways and treatment of cytokine storm in COVID-19.

Signal Transduct Target Ther 2021 07 7;6(1):255. Epub 2021 Jul 7.

Institute of Pediatrics, Children's Hospital of Fudan University, National Children's Medical Center, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

The Coronavirus Disease 2019 (COVID-19) pandemic has become a global crisis and is more devastating than any other previous infectious disease. It has affected a significant proportion of the global population both physically and mentally, and destroyed businesses and societies. Current evidence suggested that immunopathology may be responsible for COVID-19 pathogenesis, including lymphopenia, neutrophilia, dysregulation of monocytes and macrophages, reduced or delayed type I interferon (IFN-I) response, antibody-dependent enhancement, and especially, cytokine storm (CS). The CS is characterized by hyperproduction of an array of pro-inflammatory cytokines and is closely associated with poor prognosis. These excessively secreted pro-inflammatory cytokines initiate different inflammatory signaling pathways via their receptors on immune and tissue cells, resulting in complicated medical symptoms including fever, capillary leak syndrome, disseminated intravascular coagulation, acute respiratory distress syndrome, and multiorgan failure, ultimately leading to death in the most severe cases. Therefore, it is clinically important to understand the initiation and signaling pathways of CS to develop more effective treatment strategies for COVID-19. Herein, we discuss the latest developments in the immunopathological characteristics of COVID-19 and focus on CS including the current research status of the different cytokines involved. We also discuss the induction, function, downstream signaling, and existing and potential interventions for targeting these cytokines or related signal pathways. We believe that a comprehensive understanding of CS in COVID-19 will help to develop better strategies to effectively control immunopathology in this disease and other infectious and inflammatory diseases.
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http://dx.doi.org/10.1038/s41392-021-00679-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261820PMC
July 2021

[Preparation and chromatographic properties of 1-vinyl-3-dodecylimidazole bromide silica-bonded stationary phase].

Se Pu 2020 Nov;38(11):1263-1269

College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.

As the main components of cell membranes, lipids play important roles in organisms. Lipids have been proved to be closely associated with the occurrence and development of serious diseases, such as cancers and metabolic diseases. The development of novel separation materials for use in high-performance liquid chromatography (HPLC) is essential for high-efficiency lipid separation. Such materials can promote further in-depth research of the structure and biological functions of lipids. In this study, we focused on the preparation of an ionic-liquid-modified silica-bonded HPLC stationary phase and on its chromatographic retention mechanisms and separation performances for lipids. An imidazolium-based ionic liquid with C side chain, viz. 1-vinyl-3-dodecylimidazole bromide (VDI), has shown good biocompatibility and has previously been used for the solubilization of membrane proteins. Thus, VDI was first exploited as the functionalized monomer for the HPLC stationary phase. It was grafted onto the surface of thiol-functionalized silica spheres by a one-step click reaction to afford a new VDI silica-bonded stationary phase (Sil-VDI). Fourier-transform infrared (FT-IR)spectroscopy and thermogravimetric analysis were used to prove the successful preparation of Sil-VDI and characterize its structure. The chromatographic retention properties of the column packed with Sil-VDI was first studied using hydrophobic alkylbenzenes. The results showed that the Sil-VDI column was a typical reversed-phase liquid chromatography retention column. Since Sil-VDI has a permanent cationic imidazole structure, it should demonstrate anion exchange retention. Thus, inorganic anions BrO, NO, and IO were selected to further investigate the retention mechanism of the Sil-VDI column. The results demonstrate that the Sil-VDI column also possesses ion-exchange retention mode. Thus, the Sil-VDI column has typical reversed-phase and ion-exchange mixed-mode retention characteristics. Based on the reversed-phase retention characteristics of the Sil-VDI column, hydrophobic alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), basic anilines, and benzene derivatives were selected for testing the HPLC separation performances of the Sil-VDI column. The results demonstrate that this new column gave good separation selectivity with good peak shapes. For example, PAHs like diphenyl, -terphenyl, -terphenyl, and triphenylene were used to investigate the chromatographic performances of the Sil-VDI column. These four PAHs were baseline separated within 7 min with good peak shapes. In addition, the positional isomers -terphenyl and -terphenyl show good separation efficiency, with resolution as high as 3.26. Based on the ion-exchange retention characteristics of the Sil-VDI column, inorganic anions BrO, NO, and IO were selected to test the separation performance of the column for ionic compounds. Using 250 mmol/L KCl solution (pH 4.1) as the mobile phase, baseline separation of the three anions was achieved within 6 min. These results demonstrate that the Sil-VDI column has good potential for separation of ionic compounds. The separation performance of the Sil-VDI column was further verified based on the separation of lipids extracted from egg yolk and lung adenocarcinoma cells. Six main chromatographic peaks could be recognized within 7 and 5 min for the lipids extracted from lung adenocarcinoma cells and egg yolk, respectively. These results primarily demonstrate that the Sil-VDI column has good potential for the separation of lipid samples. In conclusion, a new ionic-liquid-based Sil-VDI stationary phase material was successfully fabricated via a simple synthesis method. The Sil-VDI column shows good separation performances for versatile samples. In future, further research will be performed on the separation ability of the Sil-VDI column for different biological samples.
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http://dx.doi.org/10.3724/SP.J.1123.2020.02012DOI Listing
November 2020

Growing in Mixed Stands Increased Leaf Photosynthesis and Physiological Stress Resistance in Moso Bamboo and Mature Chinese Fir Plantations.

Front Plant Sci 2021 20;12:649204. Epub 2021 May 20.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, China.

Mixed-stand plantations are not always as beneficial for timber production and carbon sequestration as monoculture plantations. Systematic analyses of mixed-stand forests as potential ideal plantations must consider the physiological-ecological performance of these plantations. This study aimed to determine whether mixed moso bamboo ( (Pradelle) Mazel ex J. Houz.) and Chinese fir ( (Lamb.) Hook.) stands exhibited better physiological-ecological performance than monoculture plantations of these species. We analyzed leaf photosynthesis, chlorophyll fluorescence, antioxidant enzyme activities, chlorophyll content and leaf chemistry in a moso bamboo stand, a Chinese fir stand and a mixed stand with both species. The results showed that both species in the mixed stand exhibited significantly higher leaf net photosynthesis rate (Amax), instantaneous carboxylation efficiency (CUE), chlorophyll content, maximum quantum yield of photosynthesis (Fv/Fm), photochemical quenching coefficient (qP), PSII quantum yield [Y(II)], leaf nitrogen content, and antioxidant enzyme activities than those in the monoculture plantations. However, the non-photochemical quenching (NPQ) in Chinese fir and 2-year-old moso bamboo was significantly lower in the mixed stand than in the monocultures. In addition, the water use efficiency (WUE) of Chinese fir was significantly higher in the mixed stand. The results suggest that the increase in leaf net photosynthetic capacity and the improved growth in the mixed stand could be attributed primarily to the (i) more competitive strategies for soil water use, (ii) stronger antioxidant systems, and (iii) higher leaf total nitrogen and chlorophyll contents in the plants. These findings suggest that mixed growth has beneficial effects on the leaf photosynthesis capacity and physiological resistance of moso bamboo and Chinese fir.
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http://dx.doi.org/10.3389/fpls.2021.649204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173113PMC
May 2021

A triple combination of treatments on moderate COVID-19.

Open Med (Wars) 2021 14;16(1):769-772. Epub 2021 May 14.

Department of Intensive Care Unit, The Affiliated Yangming Hospital of Ningbo University, Yuyao People's Hospital of Zhejiang Province, Zhejiang, China.

Objective: A triple combination of interferon (IFN) α-2b, lopinavir tablets, and umifenovir was used to treat COVID-19 patients. It is important to explore whether the benefit of this therapy is time dependent.

Methods: A cohort of moderate COVID-19 patients ( = 54) was admitted for hospitalization. The demographic (age, gender, and smoking status) and clinical characteristics (epidemiological trace and comorbidity) were collected from the digital medical records. The length of hospital stay (LOS) and the viral shedding time (VST) were set as the outcomes for COVID-19 cases.

Results: After control for age, sex, epidemiological trace, smoking, and comorbidity, the time of treatment start had null effect on VST (IRR = 1.09; 95% CI = 0.91-1.30; = 0.33) or LOS (IRR = 1.10; 95% CI = 0.94-1.28; = 0.23).

Conclusion: There is no convincing evidence to support a pivotal role of the timing of the therapy in the prognosis of moderate COVID-19 cases.
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http://dx.doi.org/10.1515/med-2021-0279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122460PMC
May 2021

Merging Imidazolidines with a Trifluoromethylated Tetrasubstituted Carbon through Tungsten Catalyzed 1,3-Dipolar Cycloaddition.

J Org Chem 2021 Jun 19;86(11):7714-7724. Epub 2021 May 19.

Institute of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, People's Republic of China.

An unprecedented 1,3-dipole cycloaddition between acyclic CF-ketimines and -benzyl azomethine ylide has been allowed by tungsten catalysis, furnishing a range of novel imidazolidines bearing a trifluoromethylated tetrasubstituted carbon center. This reaction appears as one of rare examples that challenging acyclic CF-ketimines have been engaged in 1,3-cycloaddition reactions. The capability for gram-scale synthesis and variant derivatizations of cycloaddition adducts illustrates the synthetic potential of this approach. This protocol provides a facile access to a rapidly enlarging pool of motifs with a trifluoromethylated fully substituted carbon.
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http://dx.doi.org/10.1021/acs.joc.1c00676DOI Listing
June 2021

Influence of High-Intensity Focused Ultrasound (HIFU) Ablation on Arteries: Studies.

Micromachines (Basel) 2021 Apr 25;12(5). Epub 2021 Apr 25.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore.

High-intensity focused ultrasound (HIFU) has been used to ablate solid tumors and cancers. Because of the hypervascular structure of the tumor and circulating blood inside it, the interaction between the HIFU burst and vessel is a critical issue in the clinical environment. Influences on lesion production and the potential of vessel rupture were investigated in this study for the efficiency and safety of clinical ablation. An extracted porcine artery was embedded in a transparent polyacrylamide gel phantom, with bovine serum albumin (BSA) as an indicator of the thermal lesion, and degassed water was driven through the artery sample. The HIFU focus was aligned to the anterior wall, middle of the artery, and posterior wall. After HIFU ablation, the produced lesion was photographically recorded, and then its size was quantified and compared with that in the gel phantom without artery. In addition, the bubble dynamics (i.e., generation, expansion, motion, and shrinkage of bubbles and their interaction with the artery) were captured using high-speed imaging. It was found that the presence of the artery resulted in a decrease in lesion size in both the axial and lateral directions. The characteristics of the lesion are dependent on the focus alignment. Acoustic and hydrodynamic cavitation play important roles in lesion production and interaction with the artery. Both thermal and mechanical effects were found on the surface of the artery wall after HIFU ablation. However, no vessel rupture was found in this study.
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http://dx.doi.org/10.3390/mi12050485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145754PMC
April 2021

Synchronization of memristive neural networks with unknown parameters via event-triggered adaptive control.

Neural Netw 2021 Jul 22;139:255-264. Epub 2021 Mar 22.

School of Artificial Intelligence and Automation, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China, Wuhan 430074, China. Electronic address:

This paper considers the drive-response synchronization of memristive neural networks (MNNs) with unknown parameters, where the unbounded discrete and bounded distributed time-varying delays are involved. Aiming at the unknown parameters of MNNs, the updating law of weight in response system and the gain of adaptive controller are proposed to realize the synchronization of delayed MNNs. In view of the limited communication and bandwidth, the event-triggered mechanism is introduced to adaptive control, which not only decreases the times of controller update and the amount of data sending out but also enables synchronization when parameters of MNNs are unknown. In addition, a relative threshold strategy, which is relative to fixed threshold strategy, is proposed to increase the inter-execution intervals and to improve the control effect. When the parameters of MNNs are known, the algebraic criteria of synchronization are established via event-triggered state feedback control by exploiting inequality techniques and calculus theorems. Finally, one simulation is presented to validate the effectiveness of the proposed results.
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http://dx.doi.org/10.1016/j.neunet.2021.02.029DOI Listing
July 2021

Topographical reorganization of brain functional connectivity during an early period of epileptogenesis.

Epilepsia 2021 May 15;62(5):1231-1243. Epub 2021 Mar 15.

Department of Neurology, University of California, Los Angeles, Los Angeles, California, USA.

Objective: The current study aims to investigate functional brain network representations during the early period of epileptogenesis.

Methods: Eighteen rats with the intrahippocampal kainate model of mesial temporal lobe epilepsy were used for this experiment. Functional magnetic resonance imaging (fMRI) measurements were made 1 week after status epilepticus, followed by 2-4-month electrophysiological and video monitoring. Animals were identified as having (1) developed epilepsy (E+, n = 9) or (2) not developed epilepsy (E-, n = 6). Nine additional animals served as controls. Graph theory analysis was performed on the fMRI data to quantify the functional brain networks in all animals prior to the development of epilepsy. Spectrum clustering with the network features was performed to estimate their predictability in epileptogenesis.

Results: Our data indicated that E+ animals showed an overall increase in functional connectivity strength compared to E- and control animals. Global network features and small-worldness of E- rats were similar to controls, whereas E+ rats demonstrated increased small-worldness, including increased reorganization degree, clustering coefficient, and global efficiency, with reduced shortest pathlength. A notable classification of the combined brain network parameters was found in E+ and E- animals. For the local network parameters, the E- rats showed increased hubs in sensorimotor cortex, and decreased hubness in hippocampus. The E+ rats showed a complete loss of hippocampal hubs, and the appearance of new hubs in the prefrontal cortex. We also observed that lesion severity was not related to epileptogenesis.

Significance: Our data provide a view of the reorganization of topographical functional brain networks in the early period of epileptogenesis and how it can significantly predict the development of epilepsy. The differences from E- animals offer a potential means for applying noninvasive neuroimaging tools for the early prediction of epilepsy.
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http://dx.doi.org/10.1111/epi.16863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302261PMC
May 2021

Ultrafine particulate air pollution and pediatric emergency-department visits for main respiratory diseases in Shanghai, China.

Sci Total Environ 2021 Jun 11;775:145777. Epub 2021 Feb 11.

Department of Respiratory Medicine, Children's Hospital of Fudan University, Shanghai 201102, China. Electronic address:

Background: Few studies have explored the short-term effects of ultrafine particles (UFPs, particles < 0.1 μm) air pollution on the exacerbations of pediatric respiratory diseases.

Objectives: We aimed to evaluate short-term association between UFP and emergency-department visits (EDVs) for main pediatric respiratory diseases.

Methods: We collected daily data on UFP and pediatric EDVs for main respiratory diseases [asthma, pneumonia, bronchitis and upper respiratory tract infections (URTI)] from 66 hospitals in Shanghai, China from 2016 to 2018. Generalized additive models combined with polynomial distributed lag models were applied to explore the associations between UFP level and pediatric EDVs for respiratory diseases. We fitted two-pollutant models with criteria air pollutants and performed stratified analyses by gender and age.

Results: UFP was associated with increased EDVs for all respiratory diseases in cumulative lags up to 2 d and 3 d. The greatest risk was found at cumulative lags (0-2 d) for all respiratory diseases. At cumulative lags (0-2 d), an interquartile range increase in concentrations of UFP (1800 particles/cm) was associated with relative risks of EDVs due to asthma [1.35, 95% confidence interval (CI): 1.14-1.59], pneumonia (1.20, 95% CI: 1.04-1.38), bronchitis (1.17, 95% CI: 1.03-1.33) and URTI (1.14, 95% CI: 1.02-1.28). These associations were almost unchanged when controlling for criteria air pollutants, and there was no threshold below which the associations were not present. There were stronger associations in children aged 0-13 years.

Conclusions: Short-term exposure to UFP may independently increase the risks of EDVs for asthma, pneumonia, bronchitis and URTI exacerbations among children.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145777DOI Listing
June 2021

Glycyrrhetinic Acid Protects Renal Tubular Cells against Oxidative Injury via Reciprocal Regulation of JNK-Connexin 43-Thioredoxin 1 Signaling.

Front Pharmacol 2021 2;12:619567. Epub 2021 Feb 2.

Division of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

The incidence of chronic kidney disease (CKD) is steadily increasing. Although renal tubular epithelium injury is closely correlated with the prognosis of CKD, the underlying mechanism is not fully understood and therapeutic strategies are limited. The main bioactive component of the Chinese medicine herb, glycyrrhiza, is 18α-glycyrrhetinic acid (Ga), which is also a pharmacological inhibitor of gap junctions. Our previous studies indicated that Ga is able to ameliorate renal cell injury. The present study explored the regulatory role of Ga in redox signaling in renal tubular epithelial cells with oxidative injury. Rat renal tubular epithelial cells, NRK-52E, were incubated with Px-12, a thioredoxin inhibitor, to mimic thioredoxin deficiency and induce oxidative injury . A Cell Counting Kit-8 was used to analyze cell viability while a reactive oxygen species (ROS)/superoxide (O ) fluorescence probe was employed to determine oxidative stress. Apoptosis was evaluated using DT-mediated dUTP nick end labeling/4,6-diamidino-2-phenylindole staining and cleaved caspase 3 protein analysis. Western blot analysis was used to analyze the expression of specific proteins while siRNA transfection was performed to downregulate targeted proteins. Inhibition of thioredoxin 1 by Px-12 triggered renal tubular cell oxidative injury as evidenced by morphological change, loss of cellular viability, over production of ROS and O , and appearance of cleaved caspase-3. Ga significantly attenuated cell oxidative injury, as indicated by the parameters mentioned above. Px-12 induced phosphorylation of c-Jun N-terminal kinase (JNK) and subsequently the expression of connexin 43 (Cx43) in NRK-52E cells. Ga and the JNK inhibitor, sp600125, markedly suppressed Px-12-induced generation of intracellular ROS and O . Inhibition of JNK improved Px-12-elicited NRK-52E cell injury. Moreover, sp600125 inhibited Cx43 expression. After downregulation of Cx43 via Cx43 siRNA transfection, the phosphorylation of JNK was markedly reduced. Furthermore, Ga restored the expression of thioredoxin 1 inhibited by Px-12. ROS-JNK-Cx43-thioredoxin 1 signaling plays a crucial role in renal tubular cell injury. JNK is involved in the regulation of thioredoxin 1 and Cx43, and Cx43 reciprocally regulates thioredoxin 1. Inhibition of gap junctions by Ga alleviated renal tubular oxidative injury via improvement of thioredoxin 1-mediated redox signaling.
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http://dx.doi.org/10.3389/fphar.2021.619567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884636PMC
February 2021

Highly Photoluminescent CsPbBr/CsPbBr [email protected] Nanocomposite in Light-Emitting Diodes.

Inorg Chem 2021 Mar 11;60(6):3814-3822. Epub 2021 Feb 11.

College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China.

All-inorganic halide perovskite (CsPbBr) nanocrystals (NCs) have received widespread attention owing to their unique photoelectric properties. This work reports a novel strategy to control the phase transition from CsPbBr to CsPbBr and investigates the effects of different treatment times and treatment temperatures on perovskite NCs formation. By controlling the volume of tetraethoxysilane (TEOS) added, the formation of different phases of perovskite powder can be well controlled. In addition, a white light-emitting diode (WLED) device is designed by coupling the CsPbBr/CsPbBr-CsPbBr [email protected] nanocomposite and CaAlSiN:Eu commercial phosphor with a 460 nm InGaN blue chip, exhibiting a high luminous efficiency of 57.65 lm/W, color rendering index (CRI) of 91, and a low CCT of 5334 K. The CIE chromaticity coordinates are (0.3363, 0.3419). This work provides a new strategy for the synthesis of CsPbBr/CsPbBr-CsPbBr [email protected] nanocomposite, which can be applied to the field of WLEDs and display devices.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03573DOI Listing
March 2021

Rapid Detection of , , and Genes by Loop-Mediated Isothermal Amplification in .

Foodborne Pathog Dis 2021 04 28;18(4):290-296. Epub 2021 Jan 28.

Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.

remains a serious threat to the worldwide swine industry and human health. In this study, rapid assays for the detection of three common virulence-related factors (, , and ) were developed, evaluated, and applied. Loop-mediated isothermal amplification (LAMP) primers were designed using Primer Explorer V5 software. The sensitivity and specificity of the LAMP assays were determined based on sample turbidity. For all three genes, LAMP assays were performed at 62°C with a reaction time of 60 min. The detection limit of conventional polymerase chain reaction (PCR) was 1 ng/μL, 10 pg/μL, and 100 fg/μL for the , , and genes, respectively. For the LAMP assays, the detection limits were 10 pg/μL, 10 fg/μL, and 100 fg/μL for , , and , respectively, representing sensitivities 100-1000 times higher than those of the PCR assay. Furthermore, when the LAMP assays were applied to clinical strains, the results were consistent with those of the PCR assay, confirming the LAMP assays as rapid and reliable detection techniques. In conclusion, the LAMP assays described in this study have the potential to become standard methods to detect the virulence factors , , and . To the best of our knowledge, this is the first study to report the application of LAMP to detect the , , and genes.
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http://dx.doi.org/10.1089/fpd.2020.2868DOI Listing
April 2021

[email protected] core-shell stationary phase: Preparation and its applications for mixed-mode chromatographic separation.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jan 19;1163:122506. Epub 2020 Dec 19.

College of Pharmaceutical Sciences, Key Laboratory of Public Health Safety of Hebei Province, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China. Electronic address:

The development of versatile mixed-mode stationary phase materials is of important meanings for solving the increasing demands for real sample analysis. Herein, with 2,5-dihydroxyterephthalic acid as the organic ligand and nickel as the metal centre, MOF-74 nanocrystal materials were facilely grafted on the surface of carboxyl-functionalized silica gel via layer-by-layer assembling technique. The structures of the monodisperse [email protected] material were proved by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, elemental analysis, thermogravimetric analysis, and Brunauer-Emmett-Teller specific surface area and pore size analyzer, respectively. Because the introduced 2,5-dihydroxyterephthalic acid is of hydrophilic carboxyl and hydroxyl groups, the packed [email protected] column reveals hydrophilic interaction/reversed-phase mixed-mode retention properties. Compared with commercial C column or silica-based column, the [email protected] column shows distrinct separation selectivity in short separation time for polycyclic aromatic hydrocarbons, phenolic compounds and polar sulfonamide compounds. The developed [email protected] column was further successfully applied for the separation and detection of illegal addition of glucocorticoid in children's face cream as well as sulfonamides veterinary drug residues in pure milk. The research provides a simple and convenient approach to prepare multifunctional MOFs-based stationary phase materials.
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http://dx.doi.org/10.1016/j.jchromb.2020.122506DOI Listing
January 2021

Effects of Different Management Practices on the Increase in Phytolith-Occluded Carbon in Moso Bamboo Forests.

Front Plant Sci 2020 3;11:591852. Epub 2020 Dec 3.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.

Phytolith-occluded carbon (PhytOC), a promising long-term biogeochemical carbon sequestration mode, plays a crucial role in the global carbon cycle and the regulation of atmospheric CO. Previous studies mostly focused on the estimation of the content and storage of PhytOC, while it remains unclear about how the management practices affect the PhytOC content and whether it varies with stand age. Moso bamboo ( var. ) has a great potential in carbon sequestration and is rich in PhytOC. Here, we selected four management treatments, including control (CK), compound fertilization (CF), silicon (Si) fertilization (SiF) (monosilicic acid can form phytoliths through silicification), and cut to investigate the variation of phytoliths and PhytOC contents in soil, leaves, and litters, and their storage in Moso bamboo forests. In soil, the SiF fertilizer treatment significantly ( < 0.05) increased phytolith content, PhytOC content, and storage compared to CK, while there were no significant differences between the treatments of CF and cut. In leaf, compared with CK, phytolith content of the second-degree leaves under SiF and the first-degree leaves under cut treatment significantly increased, and the three treatments significantly increased PhytOC storage for leaves with three age classes. In litter, the phytolith and PhytOC contents under the three treatments were not significantly different from that under the CK treatment. The PhytOC storage increased by 19.33% under SiF treatment, but significantly decreased by 40.63% under the CF treatment. For the entire Moso bamboo forest ecosystems, PhytOC storage of all the three management treatments increased compared with CK, with the largest increase by 102% under the SiF treatment. The effects of management practices on the accumulation of PhytOC varied with age. Our study implied that Si fertilization has a greater potential to significantly promote the capacity of sequestration of carbon in Moso bamboo forests.
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http://dx.doi.org/10.3389/fpls.2020.591852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744606PMC
December 2020

Quasisynchronization of Memristive Neural Networks With Communication Delays via Event-Triggered Impulsive Control.

IEEE Trans Cybern 2020 Dec 9;PP. Epub 2020 Dec 9.

This article considers the quasisynchronization of memristive neural networks (MNNs) with communication delays via event-triggered impulsive control (ETIC). In view of the limited communication and bandwidth, we adopt a novel switching event-triggered mechanism (ETM) that not only decreases the times of controller update and the amount of data sent out but also eliminates the Zeno behavior. By using an appropriate Lyapunov function, several algebraic conditions are given for quasisynchronization of MNNs with communication delays. More important, there is no restriction on the derivation of the Lyapunov function, even if it is an increasing function over a period of time. Then, we further propose a switching ETM depending on communication delays and aperiodic sampling, which is more economical and practical and can directly avoid Zeno behavior. Finally, two simulations are presented to validate the effectiveness of the proposed results.
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http://dx.doi.org/10.1109/TCYB.2020.3035358DOI Listing
December 2020

Temperature changes between neighboring days and childhood asthma: a seasonal analysis in Shanghai, China.

Int J Biometeorol 2021 Jun 23;65(6):827-836. Epub 2020 Nov 23.

Department of Respiratory Medicine, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.

Few evidences are available about the impact of temperature variation on childhood asthma in different seasons. This study aimed to assess the influence of temperature changes between neighboring days (TCN) on the exacerbation of asthma among children. Daily outpatient visits for childhood asthma (DOVCA) were collected from 17 main hospitals in Shanghai, China, from 2016 to 2018. A quasi-Poisson regression combined with distributed lagged nonlinear models was employed to estimate the association between TCN and asthma visits in cool or warm seasons, after controlling for short- and long-term trends, day of week, holidays, daily mean temperature, daily mean relative humidity, and air pollutants. The TCN varied from - 9.6 to 6.7 °C. The relationship between TCN and DOVCA greatly varied by season. In warm seasons, positive TCN (temperature rise) was associated with higher risks of asthma outpatient visits and negative TCN (temperature drop) was associated with lower risks; the associations were present on lag 1 day and lasted for 2 weeks; the cumulative relative risk of childhood asthma over 0 to 14 days was 1.98 (95% confidence interval: 1.42, 2.76) and 0.31 (95% confidence intervals: 0.21, 0.44) comparing a TCN of 2.5 °C (5th percentile) and - 3.2 °C (95th percentile) with 0 °C, respectively. In cool seasons, neither negative nor positive TCN showed significant risks. In conclusion, temperature rise might increase the risk of childhood asthma exacerbation and temperature drop might decrease the risks in warm seasons. There were no statistically significant influences in cool seasons.
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http://dx.doi.org/10.1007/s00484-020-02057-xDOI Listing
June 2021

Choroidal Structural Changes Assessed with Swept-Source Optical Coherence Tomography after Cataract Surgery in Eyes with Diabetic Retinopathy.

J Ophthalmol 2020 29;2020:5839837. Epub 2020 Oct 29.

Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Objective: To determine the influence of phacoemulsification on choroidal vasculature in patients with diabetic retinopathy (DR) undergoing cataract surgery using swept-source optical coherence tomography (SS-OCT).

Methods: The study was conducted in 23 eyes of 23 cataract patients with mild/moderate nonproliferative diabetic retinopathy (NPDR) without diabetic macular edema (DME) and 23 age-matched controls. Choroidal thickness (CT) and choroidal vascularity index (CVI) were measured at baseline and 1 week, 1 month, and 3 months after surgery.

Results: The baseline CVI in the DR group was significantly lower than that in the control group (=0.001). CVI in DR patients after surgery significantly increased compared with preoperative values (all < 0.001 for 1 week, 1 month, and 3 months after surgery). Postoperative increase of CVI and CT in the DR group was more than in the control group, and the difference was significant 1 month and 3 months after surgery (all < 0.05).

Conclusion: Patients with mild/moderate NPDR have reduced CVI compared with nondiabetic patients at baseline; diabetic cataract surgery tended to induce more increase in CVI and CT as compared with nondiabetic patients. This trial is registered with NCT04499768.
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http://dx.doi.org/10.1155/2020/5839837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648249PMC
October 2020

Negatively Regulates Type I Interferon-Induced Inflammation by Serving as miR-199a-5p Sponge in Type 1 Diabetes Mellitus.

Front Immunol 2020 29;11:576903. Epub 2020 Sep 29.

Institute of Pediatrics, Children's Hospital of Fudan University, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Circular RNAs (circRNAs) constitute a class of covalently circular non-coding RNA molecules formed by 5' and 3' end back-splicing. The rapid development of bioinformatics and large-scale sequencing has led to the identification of functional circRNAs. Despite an overall upward trend, studies focusing on the roles of circRNAs in immune diseases remain relatively scarce. In the present study, we obtained a differential circRNA expression profile based on microarray analysis of peripheral blood mononuclear cells (PBMCs) in children with type 1 diabetes mellitus (T1DM). We characterized one differentially expressed circRNA back-spliced from the MYB Proto-Oncogene Like 2 () gene in patients with T1DM, termed as . Subsequent assays revealed that can serve as the sponge of miR-199a-5p, release its target gene, Src homology 2 (SH2)-containing protein tyrosine phosphatase 2 (SHP2), encoded by the tyrosine-protein phosphatase non-receptor type 11 gene (), and further suppress the JAK-STAT signaling pathway triggered by type I interferon (IFN-I) to inhibit macrophage-mediated inflammation, which indicates the important roles of circRNAs in T1DM and represents a promising therapeutic molecule in the treatment of T1DM.
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http://dx.doi.org/10.3389/fimmu.2020.576903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550460PMC
June 2021

Circular RNA modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.

Theranostics 2020 29;10(24):10908-10924. Epub 2020 Aug 29.

Institute of Pediatrics, Children's Hospital of Fudan University, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.

Macrophages play critical roles in the pathogenesis of type 1 diabetes mellitus (T1DM). Circular RNAs (circRNAs) are a novel class of endogenous RNAs with covalently closed loop structures, implicated in various disease processes. However, their impact on macrophage activation and T1DM pathogenesis remains elusive. circRNA expression profiles of peripheral blood mononuclear cells (PBMCs) from T1DM children were determined by whole transcriptome microarray. Bioinformatics, quantitative real-time PCR, Western blot, RNA immunoprecipitation (RIP), cell co-culture, cell proliferation, and cell apoptosis assays were performed to investigate the expression, function, and regulatory mechanisms of . The regulatory role of was evaluated in the streptozocin-induced diabetic mouse model. We identified 27 upregulated and 31 downregulated differentially expressed circRNAs in T1DM patients. , a circRNA with unknown function, was dominantly expressed in monocytes and significantly upregulated in T1DM patients. Functionally, promoted lipopolysaccharide (LPS)-induced M1 macrophage activation via enhancement of the NF-κB signaling pathway. Mechanistically, competitively interacted with HuR to impair the translation of protein phosphatase, Mg/Mn dependent 1F (PPM1F), thus alleviating the inhibitory effect of PPM1F on the NF-κB pathway. Moreover, eukaryotic initiation factor 4A-III (EIF4A3) and fused in sarcoma (FUS) coordinately regulated expression during M1 macrophage activation. In addition, could exacerbate pancreas injury in the streptozocin-induced diabetic mice by activation of M1 macrophages . is a novel positive regulator of M1 macrophage activation through the -HuR-PPM1F-NF-κB axis. Overexpression of could promote pancreatic islet injury by enhancing M1 macrophage activation and may serve as a novel potential therapeutic target for T1DM in children.
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http://dx.doi.org/10.7150/thno.48264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532688PMC
May 2021

Research Progress in Atopic March.

Front Immunol 2020 27;11:1907. Epub 2020 Aug 27.

Institute of Pediatrics, Children's Hospital of Fudan University, The Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

The incidence of allergic diseases continues to rise. Cross-sectional and longitudinal studies have indicated that allergic diseases occur in a time-based order: from atopic dermatitis and food allergy in infancy to gradual development into allergic asthma and allergic rhinitis in childhood. This phenomenon is defined as the "atopic march". Some scholars have suggested that the atopic march does not progress completely in a temporal pattern with genetic and environmental factors. Also, the mechanisms underlying the atopic march are incompletely understood. Nevertheless, the concept of the atopic march provides a new perspective for the mechanistic research, prediction, prevention, and treatment of atopic diseases. Here, we review the epidemiology, related diseases, mechanistic studies, and treatment strategies for the atopic march.
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http://dx.doi.org/10.3389/fimmu.2020.01907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482645PMC
April 2021

Mechanical properties and osteoblast proliferation of complex porous dental implants filled with magnesium alloy based on 3D printing.

J Biomater Appl 2021 May 11;35(10):1275-1283. Epub 2020 Sep 11.

Guangzhou Janus Biotechnology Co. Ltd, Guangzhou, China.

In this paper, a complex porous dental implant with biodegradable magnesium alloy was designed based on selective laser melting (SLM). Finite element analysis (FEA) was used to simulate the stress distribution of dental implant and alveolar bone in two models of preliminary and later stages of implant. The stress concentration area of dental implants was found not in the porous structure, and the weak part of mechanical properties accords with the work requirements. The porous structure of dental implants can promote the function of cancellous bone in the process of conducting the stress of the dental implant, thus improving the bearing capacity of dental implants. fatigue experiments were carried out on the experimental samples produced by 3D printing. Through the cell contrast experiment, it was proved that the decomposed Mg could reach the titanium surface smoothly through the porous structure and complete the proliferation of osteoblasts.
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http://dx.doi.org/10.1177/0885328220957902DOI Listing
May 2021

Associations of short-term exposure to air pollution and emergency department visits for pediatric asthma in Shanghai, China.

Chemosphere 2021 Jan 6;263:127856. Epub 2020 Aug 6.

Department of Respiratory Medicine, Children's Hospital of Fudan University, Shanghai, 201102, China. Electronic address:

There is limited evidence regarding the relationship between air pollution and pediatric asthma in developing countries. This study aimed to investigate the association between short-term exposure to ambient air pollutants and pediatric asthma emergency department (ED) visits in Shanghai, China. We collected data on six criteria air pollutants (PM, PM, NO, SO, CO, and O) and daily ED visits for pediatric asthma patients from 66 hospitals in Shanghai from 2016 to 2018. The generalized additive model combined with polynomial distributed lag model was applied to explore the associations. We fitted two-pollutant models and stratified the analyses by sex, age, and season. In total, we identified 108,817 emergency department visits for pediatric asthma. A 10 μg/m increase in the concentrations of PM, NO, SO, and O was significantly associated with increased risks of pediatric asthma ED visits, with relative risk of pediatric asthma of 1.011 [95% confidence interval (CI): 1.002, 1.021], 1.030 (95%CI: 1.017, 1.043), 1.106 (95%CI: 1.041, 1.174), and 1.009 (95%CI: 1.001, 1.017), respectively. The associations of NO remained robust in the two-pollutant models. There were stronger associations for older children (6-18 years) and in warm seasons. The concentration-response curves for pediatric asthma and PM, NO, SO, and O were steeper at lower and moderate concentrations but became flatter at higher concentrations. This analysis provided evidence that short-term exposure to air pollutants (PM, NO, SO, and O) could increase the risk of asthma exacerbations among children, and health benefits would be gained from improved air quality.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127856DOI Listing
January 2021

The casein kinase 2 β subunit CK2B1 is required for swollen stem formation via cell cycle control in vegetable Brassica juncea.

Plant J 2020 11 3;104(3):706-717. Epub 2020 Sep 3.

Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.

The swollen stem is a determinant of yield for the stem-type vegetable Brassica juncea that is representative of vegetative organ formation. However, the genetic mechanism underlying swollen stem formation and its regulation remains unknown. In this study, we identified a casein kinase 2 β subunit 1 (CK2B1) and revealed its role in swollen stem formation. Genotyping analysis revealed that a homozygous variation in the CK2B1 promoter is responsible for swollen stem formation, and the promoter activity of CK2B1 was significantly associated with the variations between swollen stem and non-swollen stem types. CK2B1 was exclusively located in the nucleus and expressed in the stem nodes of the plant. Swollen stem formation was blocked when CK2B1 expression was silenced, and induced in a backcross population carrying a swollen stem genotype, which indicates that CK2B1 is required for swollen stem formation. Cell numbers were increased during swollen stem formation and decreased in CK2B1-silenced expression plant, indicating that CK2B1 regulates swollen stem formation via cell division. CK2B1 directly interacted with E2Fa, a regulator of G1/S transition in the cell cycle, in which CK2 phosphorylates E2Fa. Our results revealed that CK2B1 affects swollen stem formation via the control of the cell cycle. These findings help to elucidate the signals that control swollen stem formation and provide a promising molecular target to enhance the yield of vegetative organ formation.
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http://dx.doi.org/10.1111/tpj.14958DOI Listing
November 2020

lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma through stabilizing BAZ2B pre-mRNA.

J Allergy Clin Immunol 2021 03 23;147(3):921-932.e9. Epub 2020 Jul 23.

Institute of Pediatrics, Children's Hospital of Fudan University, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China; National Health Commission Key Laboratory of Neonatal Diseases, Fudan University, Shanghai, China. Electronic address:

Background: Dysregulation of long noncoding RNAs (lncRNAs) is associated with a variety of human diseases; however, whether they have a role in childhood asthma is unknown.

Objective: We sought to determine the differential expression profiles of lncRNAs in PBMCs of children with asthma and the mechanisms underlying the effects of lncRNAs on the pathogenesis of asthma.

Methods: The differential expression profiles of lncRNAs were analyzed by transcriptome microarray. The effects and mechanisms by which lncRNAs influence macrophage activation were detected by real-time quantitative PCR, Western blot, RNase protection assay, and chromatin immunoprecipitation assay. The roles played by lncRNAs in asthma were tested in a cockroach allergen extract (CRE)-induced mouse model.

Results: We identified 719 lncRNAs that were differentially expressed in PBMCs of children with asthma, 502 of which were upregulated and 217 were downregulated. An lncRNA of unknown function, lnc-BAZ2B, was dominantly expressed in monocytes and significantly upregulated in children with asthma. lnc-BAZ2B promotes M2 macrophage activation by enhancing BAZ2B expression and exacerbated lung inflammation in an M2 macrophage-associated CRE-induced asthma model. Mechanistically, lnc-BAZ2B promoted the expression of its cis target gene BAZ2B by stabilizing its pre-mRNA. BAZ2B, a reader of H3K14ac modification, enhanced the transcription of IRF4 and promoted M2 macrophage activation. lnc-BAZ2B expression was correlated with that of BAZ2B in PBMCs from children with asthma. Baz2b knockdown could alleviate asthma severity in a CRE-induced asthma model.

Conclusion: lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma and may serve as a potential therapeutic and diagnostic target in children with asthma.
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http://dx.doi.org/10.1016/j.jaci.2020.06.034DOI Listing
March 2021
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