Publications by authors named "Yufeng Yang"

85 Publications

circRNA_0006470 promotes the proliferation and migration of gastric cancer cells by functioning as a sponge of miR-27b-3p.

Neoplasma 2021 Oct 11. Epub 2021 Oct 11.

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People's Hospital of Southern Medical University, Dongguan, Guangdong, China.

Cancer pathogenesis is influenced by epigenetic alterations mediated by circular RNAs (circRNAs). In this study, we aimed to investigate the regulatory mechanisms and cytological function of hsa_circ_0006470/miR-27b-3p in gastric cancer (GC). CircRNA and microRNA expression in cancer cells were measured by the qRT-PCR method. A dual-luciferase reporter assay was performed to validate the binding of hsa_circ_0006470 with miR-27b-3p. hsa_circ_0006470 was silenced in AGS cells, and proliferation, migration, and invasion were tested via the CCK-8 assay and Transwell system, respectively. The autophagy in GC cells was assessed by marker protein detection and transmission electron microscope. The results showed that hsa_circ_0006470 expression was significantly elevated in GC cells, which was mainly distributed in cytoplasmic components and could directly bind with miR-27b-3p in GC cells. Silencing of hsa_circ_0006470 repressed cell proliferation, migration, and invasion, which may be through regulating miR-27b-3p/Receptor tyrosine kinase-like orphan receptor 1 (ROR1). Silencing of hsa_circ_0006470 also elevated LC3II and Beclin-1 and suppressed p62 protein abundances, which subsequently induced autophagy in AGS cells. Furthermore, we found that hsa_circ_0006470 promotes phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PI3KCA) expressing by sponging miR-27b-3p. In conclusion, hsa_circ_0006470 promoted GC cell proliferation and migration through targeting miR-27b-3p and suppressing autophagy machinery.
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http://dx.doi.org/10.4149/neo_2021_210222N235DOI Listing
October 2021

Nursing students' willingness to work in geriatric care: An integrative review.

Nurs Open 2021 09 4;8(5):2061-2077. Epub 2020 Dec 4.

Sichuan Tianyi College, Chengdu, China.

Aims: This integrative review aims to explore the willingness of nursing students to work in geriatric care over the past 10 years and to explore the factors influencing nursing students to work in geriatric care.

Design: An integrative review.

Methods: Studies investigating nursing students' willingness in gerontological nursing work and related influencing factors published in English in Cochrane Library, MEDLINE, Embase, PsycINFO and CINAHL between 2010-2020 were included. Data collected in April 2020.

Results: Twenty-four studies were analysed. Most studies presented a contradictory or negative attitude about the willingness of nursing students to engage in gerontological nursing work. In most studies that rank the intention to work in nursing fields, gerontological care received the lowest or a relatively low ranking. The main factors affecting work related to gerontological nursing include prior experience caring for older adults, attitudes towards geriatrics, anxiety about ageing, clinical practice environment and living experience with older family members.
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http://dx.doi.org/10.1002/nop2.726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363346PMC
September 2021

Extraction Behavior of Indole from Simulated Wash Oil Using Halogen-Free Ionic Liquids.

ACS Omega 2021 Jun 14;6(25):16623-16630. Epub 2021 Jun 14.

College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.

Indole is an important raw material in the chemical industry, and more than 1 wt % indole is contained in wash oil. Therefore, the extraction of indole from wash oil is of much importance. The conventional separation methods generally cost much money, pollute the environment, and corrode the metallic devices due to the use of large amounts of inorganic acid and alkali solutions, and therefore, new methods should be proposed. In this work, a solvent extraction process for separating indole from simulated wash oil by five halogen-free ionic liquids (HFILs) has been designed, and the extraction behavior of indole has been evaluated. All the studied HFILs presented excellent extraction behavior for indole, and the whole separation process took no more than 5 min. For the same HFIL, the minimum residual indole contents remained the same, even if the initial indole contents changed. Among the HFILs, 1-butyl-3-methylimidazolium dimethyl phosphate ([Bmim][DMP]) has attracted more attention than other HFILs. The results showed that [Bmim][DMP] could extract over 96.9 wt % indole from the simulated wash oil, and the minimum residual indole content was as low as 2.1 g/dm. For indole, [Bmim][DMP] presented a maximum distribution coefficient of 201, which was much improved compared to other methods. The HFILs could be regenerated by using diethyl ether with ease. The regenerated HFILs could be reused, and the extraction behavior remained the same as the original HFILs. Based on FT-IR results, a mechanism of hydrogen bonds forming between HFILs and indole was proposed. In addition, the superiorities of HFILs over other separation agents in reusability, amounts needed, distribution coefficient for indole, and chemical structure were proved by comparison.
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http://dx.doi.org/10.1021/acsomega.1c02052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246705PMC
June 2021

PBK/TOPK promotes chemoresistance to oxaliplatin in hepatocellular carcinoma cells by regulating PTEN.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr;53(5):584-592

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, The Third People's Hospital of Dongguan City, Dongguan 523326, China.

Oxaliplatin (OXA) resistance limits the efficiency of treatment for hepatocellular carcinoma (HCC). Studies have shown that the PDZ-binding kinase (PBK) plays important roles in tumors. However, the role of PBK in HCC is still a problem. In this study, we explored whether PBK is involved in the chemoresistance to OXA in HCC. Expressions of PBK in six HCC cell lines and one human hepatocytes line were determined by real-time quantitative PCR and western blot analysis. SNU-182 and HepG2 cells were chosen to induce OXA resistance. PBK was silenced or overexpressed in OXA-resistant and sensitive cell lines. Then, cell proliferation, migration, and invasion were measured by cholecystokinin-8 assay and Transwell assay, respectively. The Cancer Genome Atlas dataset showed that PBK is highly expressed in HCC and signifies poor prognosis to patient with HCC. Results showed that expression of PBK in HCC cells was significantly higher than that in THLE2 cells, and it was further increased in OXA-resistant HCC cells. Silencing of PBK promoted the sensitivity of drug-resistant HCC cells to OXA. Overexpression of PBK relieved the apoptosis induced by OXA and promoted the migration and invasion of OXA-sensitive HCC cells. Thus, this study revealed that high PBK expression is correlated with OXA resistance in HCC cells, which may provide a promising therapeutic target for treating HCC.
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http://dx.doi.org/10.1093/abbs/gmab028DOI Listing
April 2021

Combined targeting of vascular endothelial growth factor C (VEGFC) and P65 using miR-27b-3p agomir and lipoteichoic acid in the treatment of gastric cancer.

J Gastrointest Oncol 2021 Feb;12(1):121-132

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Dongguan Third Clinical Hospital of Guangdong Medical University, Dongguan, China.

Background: Gastric cancer is the second leading cancer-related mortality worldwide and more effective treatment strategies are urgently needed to combat the disease. Using lipoteichoic acid (LTA) and miR-27b-3p agomir, we aimed to assess the efficacy of this combination of therapies in treating gastric cancer.

Methods: The RNA levels of miR-27b-3p, FOXO3, MET, KRAS, vascular endothelial growth factor C (VEGFC), TSC1, and P65 were analyzed by quantified-PCR (Q-PCR) and the cell viability of AGS cells was analyzed by MTT. Confirm Luciferase reporter assays were used to explore the putative miR-27b-3p binding sites and Western blot analyzed the protein level of GAPDH, VEGFC, P65, AKT, and phosphorylated-AKT (p-AKT). The level of P65 in both the cytoplasm and nucleus of AGS cells was visualized by immunofluorescence assay. Subcutaneous xenograft models of gastric cancer were established, and mice were treated with miR-27b-3p agomir, LTA, or both. Hematoxylin-eosin staining and Ki-67 immunohistochemistry analysis of tumor tissues were then performed.

Results: The results showed that the decreased expression of miR-27b-3p in gastric cancer cell lines inhibited the viability of AGS cells, and VEGFC was confirmed as the target of miR-27b-3p. In addition, ectopic expression of miR-27b-3p significantly inhibited the AKT pathway in AGS and N87 cells, and LTA suppressed the proliferation of gastric cancer cells by inhibiting the NF-κB pathway. In an established xenograft model, both miR-27b-3p agomir alone and LTA treatment alone inhibited tumor growth and treatment which combined the two showed an even stronger inhibitory effect.

Conclusions: Taken together, the combined use of LTA and miR-27b-3p agomir exhibited a synergistic effect in the treatment of gastric cancer.
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http://dx.doi.org/10.21037/jgo-21-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944161PMC
February 2021

Protective role of vitamin B6 against mitochondria damage in Drosophila models of SCA3.

Neurochem Int 2021 03 31;144:104979. Epub 2021 Jan 31.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410000, China; Guangxi Clinical Research Center for Neurological Diseases, Guilin, Guangxi, 541001, China; Department of Neurology, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, 541001, China; Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin, Guangxi, 541004, China. Electronic address:

Polyglutamine (polyQ)-mediated mitochondria damage is one of the prime causes of polyQ toxicity, which leads to the loss of neurons and the injury of non-neuronal cells. With the discovery of the crucial role of the gut-brain axis and gut microbes in neurological diseases, the relationship between visceral damage and neurological disorders has also received extensive attention. This study successfully simulated the polyQ mitochondrial damage model by expressing 78 or 84 polyglutamine-containing Ataxin3 proteins in Drosophila intestinal enterocytes. In vivo, polyQ expression can reduce mitochondrial membrane potential, mitochondrial DNA damage, abnormal mitochondrial morphology, and loose mitochondrial cristae. Expression profiles evaluated by RNA-seq showed that mitochondrial structural genes and functional genes (oxidative phosphorylation and tricarboxylic acid cycle-related) were significantly down-regulated. More importantly, Bioinformatic analyses demonstrated that pathological polyQ expression induced vitamin B6 metabolic pathways abnormality. Active vitamin B6 participates in hundreds of enzymatic reactions and is very important for maintaining mitochondria's activities. In the SCA3 Drosophila model, Vitamin B6 supplementation significantly suppressed ECs mitochondria damage in guts and inhibited cellular polyQ aggregates in fat bodies, indicating a promising therapeutic strategy for the treatment of polyQ. Taken together, our results reveal a crucial role for the Vitamin B6-mediated mitochondrial protection in polyQ-induced cellular toxicity, which provides strong evidence for this process as a drug target in polyQ diseases treatment.
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http://dx.doi.org/10.1016/j.neuint.2021.104979DOI Listing
March 2021

A Single-Chain Variable Fragment Antibody Inhibits Aggregation of Phosphorylated Tau and Ameliorates Tau Toxicity in vitro and in vivo.

J Alzheimers Dis 2021 ;79(4):1613-1629

Institute of Life Sciences, Fuzhou University, Fuzhou, Fujian Province, P. R.China.

Background: Alzheimer's disease (AD) is a common cause of dementia among elderly people. Hyperphosphorylation and aggregation of tau correlates with the clinical progression of AD; therefore, therapies targeting the aggregation of tau may have potential applications for anti-AD drug development. Several inhibitors of tau aggregation, including small molecules and antibodies, have been found to decrease the aggregation of tau and the corresponding pathology.

Objective: To screen one kind of single-chain variable fragment (scFv) antibody which could inhibit the aggregation of tau and ameliorate its cytotoxicity.

Methods/results: Using phosphorylated tau (pTau) as an antigen, we obtained a scFv antibody via the screening of a high-capacity phage antibody library. Biochemical analysis revealed that this scFv antibody (scFv T1) had a strong ability to inhibit pTau aggregation both in dilute solutions and under conditions of macromolecular crowding. ScFv T1 could also depolymerize preformed pTau aggregates in vitro. Furthermore, scFv T1 was found to be able to inhibit the cytotoxicity of extracellular pTau aggregates and ameliorate tau-mediated toxicity when coexpressed with a hTauR406W mutant in the eye of transgenic Drosophila flies.

Conclusion: This scFv T1 antibody may be a potential new therapeutic agent against AD. Our methods can be used to develop novel strategies against protein aggregation for the treatment of neurodegenerative diseases.
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http://dx.doi.org/10.3233/JAD-191266DOI Listing
September 2021

Context-dependent responses of Drosophila intestinal stem cells to intracellular reactive oxygen species.

Redox Biol 2021 02 17;39:101835. Epub 2020 Dec 17.

Institute of Life Sciences, Fuzhou University, Fuzhou, Fujian Province, 350108, China. Electronic address:

Reactive oxygen species (ROS) contribute to cellular redox environment and serve as signaling molecules. Excessive ROS can lead to oxidative stress that are involved in a broad spectrum of physiological and pathological conditions. Stem cells have unique ROS regulation while cancer cells frequently show a constitutive oxidative stress that is associated with the invasive phenotype. Antioxidants have been proposed to forestall tumor progression while targeted oxidants have been used to destroy tumor cells. However, the delicate beneficial range of ROS levels for stem cells and tumor cells under distinct contexts remains elusive. Here, we used Drosophila midgut intestinal stem cell (ISCs) as an in vivo model system to tackle this question. The ROS levels of ISCs remained low in comparison to that of differentiated cells and increased with ageing, which was accompanied by elevated proliferation of ISCs in aged Drosophila. Neither upregulation nor downregulation of ROS levels significantly affected ISCs, implicating an intrinsic homeostatic range of ROS in ISCs. Interestingly, we observed similar moderately elevated ROS levels in both tumor-like ISCs induced by Notch (N) depletion and extracellular matrix (ECM)-deprived ISCs induced by β-integrin (mys) depletion. Elevated ROS levels further promoted the proliferation of tumor-like ISCs while reduced ROS levels suppressed the hyperproliferation phenotype; on the other hand, further increased ROS facilitated the survival of ECM-deprived ISCs while reduced ROS exacerbated the loss of ECM-deprived ISCs. However, N- and mys-depleted ISCs, which resembled metastatic tumor cells, harbored even higher ROS levels and were subjected to more severe cell loss, which could be partially prevented by ectopic supply of antioxidant enzymes, implicating a delicate pro-surviving and proliferating range of ROS levels for ISCs. Taken together, our results revealed stem cells can differentially respond to distinct ROS levels under various conditions and suggested that the antioxidant-based intervention of stem cells and tumors should be formulated with caution according to the specific situations.
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http://dx.doi.org/10.1016/j.redox.2020.101835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772796PMC
February 2021

Paradoxical Mitophagy Regulation by PINK1 and TUFm.

Mol Cell 2020 11 27;80(4):607-620.e12. Epub 2020 Oct 27.

School of Life Sciences, Tianjin University, Tianjin 300072, China.

Aberrant mitophagy has been implicated in a broad spectrum of disorders. PINK1, Parkin, and ubiquitin have pivotal roles in priming mitophagy. However, the entire regulatory landscape and the precise control mechanisms of mitophagy remain to be elucidated. Here, we uncover fundamental mitophagy regulation involving PINK1 and a non-canonical role of the mitochondrial Tu translation elongation factor (TUFm). The mitochondrion-cytosol dual-localized TUFm interacts with PINK1 biochemically and genetically, which is an evolutionarily conserved Parkin-independent route toward mitophagy. A PINK1-dependent TUFm phosphoswitch at Ser222 determines conversion from activating to suppressing mitophagy. PINK1 modulates differential translocation of TUFm because p-S222-TUFm is restricted predominantly to the cytosol, where it inhibits mitophagy by impeding Atg5-Atg12 formation. The self-antagonizing feature of PINK1/TUFm is critical for the robustness of mitophagy regulation, achieved by the unique kinetic parameters of p-S222-TUFm, p-S65-ubiquitin, and their common kinase PINK1. Our findings provide new mechanistic insights into mitophagy and mitophagy-associated disorders.
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http://dx.doi.org/10.1016/j.molcel.2020.10.007DOI Listing
November 2020

An enhanced procedure for urban mobile methane leak detection.

Heliyon 2020 Oct 9;6(10):e04876. Epub 2020 Oct 9.

Boston University, Department of Earth and Environment, U.S.A.

Leaked methane from natural gas distribution pipelines is a significant human and environmental health problem in urban areas. To assess this risk, urban mobile methane leak surveys were conducted, using innovative methodology, on the streets of Hartford, Danbury, and New London, Connecticut, in March 2019. The Hartford survey was done to determine if results from a 2016 survey (Keyes et al., 2019) were persistent, and surveys in additional towns were done to determine if similar findings could be made using an identical approach. Results show that Hartford continues to be problematic, with approximately 3.4 leaks per road mile observed in 2016 and 4.3 leaks per mile estimated in 2019, similar to that previously found in Boston, Massachusetts (Phillips et al., 2013). A preliminary estimate of methane leaks in Hartford is 0.86 metric tonnes per day (or 313 metric tonnes per year), equivalent to 42,840 cubic feet per day of natural gas, and a daily gas consumption of approximately 214 U.S. households. Moreover, the surveys and analyses done for Danbury and New London also reveal problematic leaks, particularly for Danbury with an estimated 3.6 leaks per mile. Although road miles covered in New London were more limited, the survey revealed leak-prone areas, albeit with a range of methane readings lower than those in Hartford and Danbury. Data collection methods for all studies is first reported here and are readily transferable to similar urban settings. This work demonstrates the actionable value that can be gained from data-driven evaluations of urban pipeline performance, and if supplemented with a map of leak-prone pipe geo-location, and information on pipeline operating pressures, will provide a spatial database facilitating proactive repair and replacement of leak-prone urban pipes, a considerable improvement compared to reactive mitigation of human-reported leaks. While this work pertains to the selected urban towns in the Northeast, it exemplifies issues and opportunities nationwide in the United States.
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http://dx.doi.org/10.1016/j.heliyon.2020.e04876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560587PMC
October 2020

AttPNet: Attention-Based Deep Neural Network for 3D Point Set Analysis.

Sensors (Basel) 2020 Sep 23;20(19). Epub 2020 Sep 23.

Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Point set is a major type of 3D structure representation format characterized by its data availability and compactness. Most former deep learning-based point set models pay equal attention to different point set regions and channels, thus having limited ability in focusing on small regions and specific channels that are important for characterizing the object of interest. In this paper, we introduce a novel model named Attention-based Point Network (AttPNet). It uses attention mechanism for both global feature masking and channel weighting to focus on characteristic regions and channels. There are two branches in our model. The first branch calculates an attention mask for every point. The second branch uses convolution layers to abstract global features from point sets, where channel attention block is adapted to focus on important channels. Evaluations on the ModelNet40 benchmark dataset show that our model outperforms the existing best model in classification tasks by 0.7% without voting. In addition, experiments on augmented data demonstrate that our model is robust to rotational perturbations and missing points. We also design a Electron Cryo-Tomography (ECT) point cloud dataset and further demonstrate our model's ability in dealing with fine-grained structures on the ECT dataset.
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http://dx.doi.org/10.3390/s20195455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582494PMC
September 2020

LncRNA FAM230B Promotes Gastric Cancer Growth and Metastasis by Regulating the miR-27a-5p/TOP2A Axis.

Dig Dis Sci 2021 08 10;66(8):2637-2650. Epub 2020 Sep 10.

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People's Hospital of Southern Medical University, 1 Xianglong Road, Dongguan City, 510080, Guangdong Province, China.

Aim: Long non-coding RNAs serve as key components of competing endogenous RNA (ceRNA) networks that underlie tumorigenesis. We investigated the pathogenic roles of lncRNA FAM230B and its molecular mechanism in gastric cancer (GC).

Method: The levels of FAM230B expression in five gastric cancer cell lines and in human gastric mucosal cells were compared by quantitative RT-PCR. To analyze the function of FAM230B in GC, we overexpressed FAM230B in AGS cells, silenced FAM230B in MGC-803 cells, and tested the effect of FAM230B on tumor growth in nude mice. The interaction between miR-27a-5p and FAM230B was predicted by a bioinformatics analysis and then verified with a dual-luciferase reporter assay. We also further investigated the role and mechanism of FAM230B by forcing overexpression of miR-27a-5p in MGC-803 gastric cancer cells.

Results: We found that FAM230B was highly expressed in gastric cancer cell lines and mainly located in the cytoplasm. FAM230B overexpression promoted the proliferation, migration, and invasion of AGS cells and repressed their apoptosis; it also facilitated tumor growth in vivo. In contrast, FAM230B knockdown suppressed the proliferation, migration, and invasion of MGC0803 cells, but enhanced their apoptosis and inhibited tumor growth in vivo. MiR-27a-5p expression was suppressed by FAM230B overexpression in AGS cells. MiR-27a-5p inhibited the proliferation, migration, and invasion of gastric cancer cells, and promoted the apoptosis of gastric cancer cells by reducing TOP2A (topoisomerase 2 alpha) expression.

Conclusion: Our study showed that lncRNA FAM230B might function to promote GC. FAM230B functioned as a ceRNA by sponging miR-27a-5p and enhancing TOP2A expression.
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http://dx.doi.org/10.1007/s10620-020-06581-zDOI Listing
August 2021

Protection of Siganus oramin, rabbitfish, from heavy metal toxicity by the selenium-enriched seaweed Gracilaria lemaneiformis.

Ecotoxicol Environ Saf 2020 Dec 2;206:111183. Epub 2020 Sep 2.

Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.

Seaweed is an inherently important entity in marine ecosystems. It is not only consumed by aquatic animals but also improves environmental quality in the mariculture. Seaweed is also part of the diet of human beings. The purpose of the present study was to evaluate the antagonism of selenium (Se)-enriched Gracilaria lemaneiformis against heavy metals, specifically, the potential of dietary Se-enriched Gracilaria to protect against heavy metal toxicity in rabbitfish (Siganus oramin). Growth rate, heavy metal (Se, Cd, Pb, Cu, Zn and Cr) concentrations, malondialdehyde (MDA), metallothionein (MT), and the activity of the antioxidants, glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) were all assessed. The results showed that the total organic and inorganic Se concentration for the 250 mg L Se-enriched Gracilaria was significantly higher than those of the 50 and 10 mg L treatments after 3 days of enrichment. The mean total Se concentrations in Gracilaria were 42.5 μg g in the 250 mg L treatment, 13.5 μg g in the 50 mg L treatment and 2.5 μg g in the 10 mg L treatment, respectively. Organic Se accounts for 80-82% of total Se in Se-enriched Gracilaria. The Se concentration of rabbitfish fed Se-enriched Gracilaria was significantly higher than control. Furthermore, Se increased Cu and Zn absorption, and enhanced MT generation, and improved GPX, CAT, and SOD antioxidant activity, and decreased MDA concentrations and lipid peroxidation levels, all antagonistic to Cd, Pb and Cr. The effects of Se-enriched Gracilaria on waterborne Cd, Pb and Cr-induced toxicity occurred via both enzymatic and non-enzymatic antioxidative mechanisms in rabbitfish. Selenium had synergistic effects on Zn and Cu in rabbitfish. For the 50 mg L Se-enriched Gracilaria treatment, the Se, Cu, Zn, and antagonistic Cd, Pb, Cr, and the antioxidant enzymes CAT, SOD, GPX activities, and MT concentrations in rabbitfish were higher than that with the 250 mg L and 10 mg L Se-enriched Gracilaria treatments. The 50 mg L Se treatment of Gracilaria was deemed to be the optimum concentration to promote growth of rabbitfish. Therefore, the obtained results suggest Se-enriched Gracilaria can antagonize heavy metal toxicity, and is an advisable Se supplement to improve the edible safety of cultured animals.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111183DOI Listing
December 2020

Riverine deposition pattern of oil-particle aggregates considering the coagulation effect.

Sci Total Environ 2020 Oct 20;739:140371. Epub 2020 Jun 20.

School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.

To understand the heterogeneous behavior of oil-particle aggregates (OPAs) in the riverine environment as well as the uncertainties caused by the coupling effects between their stochastic formation and transportation processes, this study employed the coagulation conceptual formula and random-walk particle tracking model. Through careful inspection using the classic Rouse-Vanoni diagram and existing laboratory observations, a vertical diffusivity scheme and the packing coefficient for an oil-sediment interaction model were determined. The density variations and deposition patterns of hypothetically fully developed OPAs as well as the impact of oil-sediment interactions on the longitudinal distribution of deposited OPAs were then investigated. The results indicate that the formation process of OPAs has a significant effect on their longitudinal deposition. The range of potentially trapped OPAs varied from several to hundreds of times the range of cases that exclude oil-sediment interactions. The deposition diagram proposed in this study visualizes the relationship between the configuration and deposition pattern of OPAs and can assist in determining the most unfavorable scenarios for oil-spill countermeasures. Further refinement and calibration of the model are necessary in the future to provide guidelines for oil spill responses and recovery in riverine environments.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140371DOI Listing
October 2020

Attenuation of epigenetic regulator SMARCA4 and ERK-ETS signaling suppresses aging-related dopaminergic degeneration.

Aging Cell 2020 09 4;19(9):e13210. Epub 2020 Aug 4.

Institute of Life Sciences, Fuzhou University, Fuzhou, Fujian, China.

How complex interactions of genetic, environmental factors and aging jointly contribute to dopaminergic degeneration in Parkinson's disease (PD) is largely unclear. Here, we applied frequent gene co-expression analysis on human patient substantia nigra-specific microarray datasets to identify potential novel disease-related genes. In vivo Drosophila studies validated two of 32 candidate genes, a chromatin-remodeling factor SMARCA4 and a biliverdin reductase BLVRA. Inhibition of SMARCA4 was able to prevent aging-dependent dopaminergic degeneration not only caused by overexpression of BLVRA but also in four most common Drosophila PD models. Furthermore, down-regulation of SMARCA4 specifically in the dopaminergic neurons prevented shortening of life span caused by α-synuclein and LRRK2. Mechanistically, aberrant SMARCA4 and BLVRA converged on elevated ERK-ETS activity, attenuation of which by either genetic or pharmacological manipulation effectively suppressed dopaminergic degeneration in Drosophila in vivo. Down-regulation of SMARCA4 or drug inhibition of MEK/ERK also mitigated mitochondrial defects in PINK1 (a PD-associated gene)-deficient human cells. Our findings underscore the important role of epigenetic regulators and implicate a common signaling axis for therapeutic intervention in normal aging and a broad range of age-related disorders including PD.
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http://dx.doi.org/10.1111/acel.13210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511865PMC
September 2020

The Beta-Diversity of -Associated Microbial Communities From Different Habitats Increases With Body Weight.

Front Microbiol 2020 7;11:1562. Epub 2020 Jul 7.

Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou, China.

Fish-associated microbial communities play important roles in host growth, health and disease in the symbiont ecosystem; however, their diversity patterns and underlying mechanisms in different body habitats remain poorly understood. is one of the most important consumers of macroalgae and an excellent natural marine source of nutritional lipids for humans, and widely distributes in shallow coastal areas. Here we systematically studied the microbial communities of 108 wild in four body habitats (i.e., skin, gill, stomach, and hindgut) and surrounding water. We found that the β-diversity but not α-diversity of fish-associated microbial communities from each habitat significantly ( < 0.05) increased as body weight increased. Also, opportunistic pathogens and probiotics (e.g., , ) appeared to be widely distributed in different body habitats, and many digestive bacteria (e.g., ) in the hindgut; the abundances of some core OTUs associated with digestive bacteria, "" (OTU_6 and OTU_46724) and "" (OTU_33295) in the hindgut increased as body weight increased. Additionally, the quantification of ecological processes indicated that heterogeneous selection was the major process (46-70%) governing the community assembly of fish microbiomes, whereas the undominated process (64%) was found to be more important for the water microbiome. The diversity pattern showed that β-diversity (75%) of the metacommunity overweight the α-diversity (25%), confirming that the niche separation of microbial communities in different habitats and host selection were important to shape the fish-associated microbial community structure. This study enhances our mechanistic understanding of fish-associated microbial communities in different habitats, and has important implications for analyzing host-associated metacommunities.
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http://dx.doi.org/10.3389/fmicb.2020.01562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358552PMC
July 2020

Anti-cancer peptides: classification, mechanism of action, reconstruction and modification.

Open Biol 2020 07 22;10(7):200004. Epub 2020 Jul 22.

Department of Bioengineering, Zunyi Medical University Zhuhai Campus, Zhuhai, Guangdong 519040, People's Republic of China.

Anti-cancer peptides (ACPs) are a series of short peptides composed of 10-60 amino acids that can inhibit tumour cell proliferation or migration, or suppress the formation of tumour blood vessels, and are less likely to cause drug resistance. The aforementioned merits make ACPs the most promising anti-cancer candidate. However, ACPs may be degraded by proteases, or result in cytotoxicity in many cases. To overcome these drawbacks, a plethora of research has focused on reconstruction or modification of ACPs to improve their anti-cancer activity, while reducing their cytotoxicity. The modification of ACPs mainly includes main chain reconstruction and side chain modification. After summarizing the classification and mechanism of action of ACPs, this paper focuses on recent development and progress about their reconstruction and modification. The information collected here may provide some ideas for further research on ACPs, in particular their modification.
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http://dx.doi.org/10.1098/rsob.200004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574553PMC
July 2020

Bdelloid rotifers (Rotifera, Bdelloidea) of China: diversity and new records.

Zookeys 2020 16;941:1-23. Epub 2020 Jun 16.

Institute of Hydrobiology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China Jinan University Guangzhou China.

Bdelloid rotifers are a group of microscopic invertebrates known for their obligate parthenogenesis and exceptional resistance to extreme environments. Their diversity and distributions are poorly studied in Asia, especially in China. In order to better understand the species distribution and diversity of bdelloid rotifers in China, a scientific surveys of habitats was conducted with 61 samples (both terrestrial and aquatic habitats) from 11 provinces and regions of China, ranging from tropics to subtropics with a specific focus on poorly sampled areas (Oriental) during September 2017 to October 2018. A total of 59 morphospecies (including subspecies) were found, of which, thirty-nine morphospecies (including one genus) are new records for China, almost doubling the number of previous records. Four rare morphospecies (Adineta cf. acuticornis Haigh, e Örstan, De Koning and Donner) are depicted and redescribed, and an updated checklist of Chinese bdelloids with their location and ecological information is presented. This study provides new data from a large region of China, enriching the knowledge of bdelloid biodiversity, and their global biogeography.
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http://dx.doi.org/10.3897/zookeys.941.50465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311513PMC
June 2020

Evaluation of Liquid Versus Dry Specimen Transport With a Newly Validated Isothermal Amplification High-Risk Human Papillomavirus Assay.

J Low Genit Tract Dis 2020 Jul;24(3):243-246

Department of Obstetrics and Gynecology, Shanxi Bethune Hospital & Shanxi Academy of Medical Sciences, Taiyuan, Shanxi Province, China.

Objective: The aim of the study was to determine whether the proportion of positive high-risk human papillomavirus (HR-HPV) tests in endocervical specimens transported dry differs from paired specimens transported in liquid media.

Methods: Five hundred women aged of 30 to 55 years were recruited, Shanxi Bethune Hospital, China. Two samples were collected from the endocervix per patient, one placed into empty vial, the other into a liquid transport solution. All samples were analyzed by AmpFire HR-HPV assay.

Results: Total 1,000 samples collected from 500 patients were analyzed by the AmpFire HR-HPV assay. The total invalid rate was 0.2% (2/1,000). The proportion of endocervical samples testing positive for HR-HPV transported dry (42.2%, 210/498 [95% CI = 37.8%-46.6%]) was similar to the proportion of paired endocervical samples testing positive transported in liquid media (40.4%, 201/498 [95% CI = 36.0%-44.8%], p = .18 [McNemar test]). That the 2 transport methods are likely measuring the same positive (and negative) specimens is suggested by the finding that κ value for the correlation of positive HR-HPV in endocervical specimens transported dry with those transported in liquid media was 0.86 (95% CI = 0.81-0.90).

Conclusions: Endocervical specimens transported dry have similar proportion of positive HR-HPV tests as those transported in liquid media. Dry brush transport of endocervical samples paired with the special characteristics of AmpFire HR-HPV may become an important addition to population based cervical cancer screening.
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http://dx.doi.org/10.1097/LGT.0000000000000546DOI Listing
July 2020

Toxic effects and mechanisms of Prymnesium parvum (Haptophyta) isolated from the Pearl River Estuary, China.

Harmful Algae 2020 06 10;96:101844. Epub 2020 Jun 10.

Department of Ecology/ Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, PR China. Electronic address:

Prymnesium (Haptophyta) species form toxic blooms throughout the world resulting in large fish-kills and economic losses. A culture of Prymnesium parvum JX12 was isolated and established from the Pearl River Estuary of China. The toxic characteristics of JX12 to aquatic animals were investigated, considering the effects of temperature, salinity, and nutrients. Cultures of JX12 exhibited significant lethal toxicity to the fish, Mugil sp., M. soiuy, M. cephalus, Ctenogobius sp. and Chrysiptera parasema, and the brine shrimp, Artemia salina, but no significant effects on the shellfish Ruditapes variegatus and the shrimp Penacus orientalis. The toxicity of JX12 to A. salina increased with growth of the culture. The decline phase displayed the strongest potency, followed by the stationary phase, exponential phase and lag phase. Also, there was a significant difference in toxicity among culture fractions of JX12 compared to whole-cell culture. For example, the toxicity of a cell-free culture filtrate on brine shrimp was reduced by 50% by the end of the experiment, while the sonicated and re-suspended cultures caused near 100% mortality. The toxicity of JX12 was also regulated by environmental factors such as temperature, salinity and nutrients. The strongest toxicities were at moderate water temperature (20-25 °C) and salinity (35 psu). The toxicity was reduced significantly at higher or lower temperatures and salinities. Furthermore, the toxicity of JX12 was greatly enhanced when ambient nutrients were deficient. Due to the potent toxicity of JX12 to multiple aquatic animals, especially fish and zooplankton, this species threatens the food web and trophic dynamics of coastal ecosystems. This study suggested that the ability to produce and release toxin(s) is an effective competition strategy for P. parvum to suppress predators and sympatric competitors and thus form blooms.
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http://dx.doi.org/10.1016/j.hal.2020.101844DOI Listing
June 2020

Enhancing the nitrogen removal of anammox by treating municipal wastewater with sludge fermentation products in a continuous flow reactor.

Bioresour Technol 2020 Aug 1;310:123468. Epub 2020 May 1.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.

In this study, a novel process was developed to treat real sewage with a low chemical oxygen demand/total nitrogen ratio (COD/TN = 3.2) and to obtain enhanced nitrogen removal through Anaerobic ammonia oxidation (anammox). Anaerobic/aerobic/anoxic/aerobic (AOAO) reactor processes were amended with a fixed anammox biofilm in the anoxic zone. During an operational period of 212 days, an average effluent TN of 13.7 mg/L with a removal efficiency of 72.0% was obtained with an influent of 47.0 mg/L ammonium. Mass balance analysis suggested that the anammox resulted in removal of 33.6% of the TN. Besides, by adding sludge fermentation products, nitrite accumulation occurred via nitration while in the aerobic zone for the anammox process. This study demonstrated an alternative way to apply a sewage anammox process via excess sludge fermentation products triggering nitrite production in a continuous flow reactor.
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http://dx.doi.org/10.1016/j.biortech.2020.123468DOI Listing
August 2020

Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.

Nucleic Acids Res 2020 06;48(10):e57

State Key Laboratory of Biomembrane and Membrane Engineering, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
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http://dx.doi.org/10.1093/nar/gkaa195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261186PMC
June 2020

Association Between BAK1 Gene rs210138 Polymorphisms and Testicular Germ Cell Tumors: A Systematic Review and Meta-Analysis.

Front Endocrinol (Lausanne) 2020 23;11. Epub 2020 Jan 23.

Department of Urology Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.

Several studies including some genome-wide association studies (GWAS) had shown that BAK1 gene rs210138 polymorphisms might be associated with testicular germ cell tumors (TGCT). Here we tried to sum up the association through a systematic review and meta-analysis. Studies associated with BAK1 rs210138 and TGCT was systematically searched in databases. The effect size was pooled according to ORs and 95% CIs. Our systematic review and meta-analysis comprised 14 articles. Significantly increased risk of TGCT was found in eligible GWAS and follow-up studies, in overall group and its Caucasian subgroup. Compared with adenine (A), BAK1 rs210138 guanine (G) is associated with increased risk of TGCT. Well-planned studies with larger sample size and more subgroups are needed to verify the risk identified in our systematic review and meta-analysis.
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http://dx.doi.org/10.3389/fendo.2020.00002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989409PMC
February 2021

Ecological risk assessment of heavy metal concentrations in sediment and fish of a shallow lake: a case study of Baiyangdian Lake, North China.

Environ Monit Assess 2020 Jan 31;192(2):154. Epub 2020 Jan 31.

School of Civil Engineering, Yantai University, Yantai, China.

The pollution levels of lakes vary in quantity and type of contaminants accumulated in their sediment and water. The second Chinese capital city will be built around Baiyangdian Lake in the near future, and thus, it is important to monitor pollution status of Baiyangdian Lake. This study mainly focused on the accumulated heavy metal concentrations in the surface sediment and in variety of fish bodies. Sediment pollution status and ecological risk were evaluated through geo-accumulation (I-geo), contamination factor (CF), pollution load index (PLI), potential ecological risk ([Formula: see text]), and mean probable effect concentration quotient (mPEC-Q). In addition, human health risks via fish consumption were also evaluated. Based on the results, the average sediment trace As, Cd, Cr, Cu, Mn, Ni, Pb, and Zn concentrations were 9.53, 0.35, 56.37, 32.33, 617.05, 30.18, 19.17, and 84.24 mg/kg dry wt, respectively. Both I-geo and [Formula: see text] inferred low pollution levels and low ecological risks from all assessed trace metals except Cd. Cd posed moderate to high ecological risks. Based on sediment quality guidelines (SQGs), average Ni and Cr concentrations exceeded the threshold effect concentrations (TEC), but their [Formula: see text] are low as their average concentrations is below Hebei province pre-contaminations (30.8 mg/kg for Ni and 68.3 mg/kg for Cr). There is no cumulative toxicity from all the metals through mPEC-Q. Omnivorous fish accumulated statistically insignificantly higher amounts of metals than carnivorous fish, except for Hg. The intake of 12.22 g/person/day fish muscle for the entire life is safe from noncarcinogenic human health problems.
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http://dx.doi.org/10.1007/s10661-020-8078-8DOI Listing
January 2020

Generalized additive models for biomass simulation of submerged macrophytes in a shallow lake.

Sci Total Environ 2020 Apr 21;711:135108. Epub 2019 Nov 21.

State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875, China; Ministry of Education Key Laboratory of Water and Sediment Science, School of Environment, Beijing Normal University, Beijing 100875, China.

Submerged macrophytes are widely distributed primary producer that play important roles in maintaining healthy aquatic ecosystems. Generally, the relationships between macrophytes and environmental factors are complicated, so nonlinear nonparametric models with relatively flexible structures are optimal for macrophyte habitat simulation. In this study, generalized additive model (GAM) was used to evaluate the response of the submerged macrophytes biomass to water environmental factors in the Baiyangdian Lake. Forward stepwise method was used to implement model optimization. Likelihood ratio test was used to determine whether adding a variable enhances the model performance. Four individual variables (water depth, transparency, total nitrogen, and total phosphorus) and two interaction terms (water depth × transparency and water depth × total phosphorus) were included in the optimal GAM. The optimal model explained 70.5% of the biomass variation with a relatively low residual deviance value (22.40). There was a significant correlation between the measured and predicted data (R = 0.716, p = 0.0004). The response lines generated by the model indicated that macrophyte biomass had a positive correlation with transparency but negative correlations with total nitrogen and nitrite nitrogen in water. The response patterns of macrophyte biomass to water depth and total phosphorus were unimodal. The biomass reached the maximum value when the water depth was about 2.1 m and the total phosphorus concentration was 0.07 mg/L. Water depth and transparency, which affect light availability, are critical physical variables affecting the conditions associated with the submerged macrophytes, and excess nitrite and phosphorus limiting macrophyte biomass.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135108DOI Listing
April 2020

Influence of Hydrological Heterogeneity on Rotifer Community Structure in Three Different Water Bodies in Shantou Area, Guangdong (China).

Zool Stud 2019 16;58:e23. Epub 2019 Sep 16.

Institute of Hydrobiology, Jinan University, 601 West Huangpu Avenue, Guangzhou, Guangdong, China. Correspondence: E-mail: (Yang). E-mail: (Liang).

Rotifers, small but essential invertebrates in aquatic ecosystems, are sensitive to environmental changes and are proposed to be indicators of trophic state. However, the effects of hydrological heterogeneity on the rotifer community and the ability of rotifer indices to reflect trophic state across different water bodies are still unclear. Here, we investigated rotifer community structure in different seasons in the three types of water bodies: Han river downstream (HD), Reservoir (RE) and Tidal creek (TC) in Shantou City, Guangdong, China. Our findings revealed that rotifer community structure differes significantly among the three water bodies, resulting from a dominance of , and , who largely accounted for the differences in water bodies. Chlorophyll- and transparency were the main environmental drivers in RE rotifer communities, while total nitrogen, total phosphorus and salinity were the main factors in HD and TC communities. Rotifer abundance and the rotifer trophic state index decreased in the order: RE > HD > TC. However, both the Sladecek's B/T quotient and the - index decreased in the order: HD > RE > TC, which was in accordance with the Carlson's trophic index. We conclude that it is efficient to use rotifer composition in water quality assessments when comparing different water bodies. Alpha diversity of rotifers was the highest in HD, which is consistent with the intermediate disturbance hypothesis. Hydrological heterogeneity is the micro-factor that regulates rotifer community structures in the Shantou area.
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http://dx.doi.org/10.6620/ZS.2019.58-23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917561PMC
September 2019

Transcript profiling for regulation of sweet potato skin color in Sushu8 and its mutant Zhengshu20.

Plant Physiol Biochem 2020 Mar 28;148:1-9. Epub 2019 Dec 28.

Department of Horticulture, University of Arkansas, Fayetteville, AR72701, USA. Electronic address:

Sweet potato [Ipomoea batatas (L.) Lam.] (2n = 6x = 90) is an economic important autopolyploid species and its varieties differ regarding storage root skin and flesh colors. Two sweet potato genetic lines, Sushu8 (with red skin) and its mutant Zhengshu20, which produced different colored storage roots, were used in this study. The total flavonoid, carotenoid, and anthocyanin contents of the two lines were analyzed and revealed that anthocyanin was primarily responsible for the skin color difference. In addition, the early storage root expanding stage was the key period for anthocyanin accumulation in Sushu8. A total of 24 samples, including the skins of the fibrous root and the storage root at the early and middle expanding stages as well as the flesh of the storage root at the middle expanding stage, were analyzed based on differentially expressed genes identified by transcriptome sequencing and a weighted gene co-expression network analysis. Two gene modules highly related with the regulation of sweet potato skin color through stress responses as well as starch synthesis and glucose metabolism were identified. Furthermore, the WRKY75 transcription factor gene, fructose-bisphosphate aldolase 2 gene, and other DEGs highly related to the regulation of anthocyanin metabolism were enriched in the brown and green modules.
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http://dx.doi.org/10.1016/j.plaphy.2019.12.035DOI Listing
March 2020

Potential bioremediation effects of seaweed Gracilaria lemaneiformis on heavy metals in coastal sediment from a typical mariculture zone.

Chemosphere 2020 Apr 11;245:125636. Epub 2019 Dec 11.

Institute of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms, Guangdong Higher Education Institutes, Jinan University, Guangzhou, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, China. Electronic address:

Seaweeds are good bio-monitors of heavy metals pollution in coastal seawater. In the present study, the potential bioremediation effects of cultivated Gracilaria lemaneiformis on heavy metals in Nan'ao coastal sediment from a typical mariculture zone, South China were evaluated. Sediment samples were collected from five different zones (Gracilaria cultivation zone, G; Fish culture zone, F; Shellfish culture zone, S; Transition zone, T; Control zone, C) from December 2014 to July 2015. The concentrations of Cd, Pb, Cu, and Zn in the sediments were significantly different among the various types of mariculture areas. The concentrations varied widely: Cd (0.04-1.02) μg g; Cu (1.19-37.70) μg g; Pb (8.45-74.45) μg g; Zn (36.80-201.24) μg g. The lowest heavy metal concentrations in the sediment were occurred at Gracilaria cultivation zone, while higher concentrations occurred at control zones and fish culture zones. The pollution load index, principal components and cluster analysis showed that heavy metal concentrations were the highest at fish culture zone, while the concentrations were the lowest at Gracilaria cultivation zone, and Gracilaria cultivation affects the heavy metals in the sediments. Gracilaria had strong adsorption capacities for heavy metals from seawater, showing the highest heavy metal Bioconcentration Factors in May (higher seaweed biomass period). Consequently, the results suggested that Gracilaria cultivation influences the heavy metal concentrations in sediments from the typical coastal mariculture zone. Gracilaria cultivation has the potential to bioremediate heavy metals in the coastal sediments. Therefore, Gracilaria cultivation can add environmental advantages and ecological values to coastal mariculture zones.
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http://dx.doi.org/10.1016/j.chemosphere.2019.125636DOI Listing
April 2020

High-Salt Diet Causes Sleep Fragmentation in Young Through Circadian Rhythm and Dopaminergic Systems.

Front Neurosci 2019 29;13:1271. Epub 2019 Nov 29.

Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou, China.

Salt (sodium chloride) is an essential dietary requirement, but excessive consumption has long-term adverse consequences. A high-salt diet (HSD) increases the risk of chronic diseases such as cardiovascular conditions and diabetes and is also associated with poor sleep quality. Little is known, however, about the neural circuit mechanisms that mediate HSD-induced sleep changes. In this study, we sought to identify the effects of HSD on the sleep and related neural circuit mechanisms of . Strikingly, we found that HSD causes young to exhibit a fragmented sleep phenotype similar to that of normal aging individuals. Importantly, we further showed that HSD slightly impairs circadian rhythms and that the HSD-induced sleep changes are dependent on the circadian rhythm system. In addition, we demonstrated that HSD-induced sleep changes are dopaminergic-system dependent. Together, these results provide insight into how elevated salt in the diet can affect sleep quality.
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http://dx.doi.org/10.3389/fnins.2019.01271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895215PMC
November 2019

Rotifers from China (Western Guangdong Province), with description of Lecane zhanjiangensis sp. nov. (Rotifera: Monogononta: Lecanidae).

Zootaxa 2019 May 8;4603(1):zootaxa.4603.1.3. Epub 2019 May 8.

Department of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou 510632, China..

Littoral rotifers were collected in three prefectural cities of the little studied Western Guangdong Province (Yangjiang, Maoming and Zhanjiang) in April 2017 and in July 2018. Fifty-six monogonont taxa, including two subspecies/variations, were found. Lecane zhanjiangensis sp. nov., a new brackish-water species from the littoral zone of Shimenhe River Estuary in Zhanjiang, is described. It is characterized by large semicircular projections antero-laterally on the dorsal plate, each equipped with three to five minute spines, and a pair of large acutely pointed projections antero-laterally on the ventral head aperture margin. In addition, three new rotifer records for China, viz. Brachionus cf. caudatus var. indica Novotná-Dvořáková, 1963, Ptygura libera Myers, 1934 and P. thalenoiensis Meksuwan, Pholpunthin Segers, 2011 are also presented. [Species zoobank registraion: http://zoobank.org/urn:lsid:zoobank.org:act:E91C0C2A-8656-4999-B71C-00F39456F7A0].
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http://dx.doi.org/10.11646/zootaxa.4603.1.3DOI Listing
May 2019
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