Publications by authors named "Yufei Wang"

373 Publications

Cement-associated thrombotic embolism in the inferior vena cava and bilateral iliac veins after percutaneous vertebroplasty: a case report.

J Int Med Res 2021 Sep;49(9):3000605211046115

The Department of Spine Surgery, The People's Hospital of Liuyang City, Changsha, Hunan Province, China.

Percutaneous vertebroplasty (PVP) was first reported in 1987 for treating vertebral hemangiomas. PVP is also an effective treatment for osteoporotic vertebral compression fracture that mainly involves a percutaneous injection of polymethylmethacrylate. Severe complications of PVP have been reported in recent years. However, to the best of our knowledge, cement-associated thrombotic embolism in the inferior vena cava and bilateral iliac veins after PVP has rarely been reported. We experienced a patient with cement-associated thrombotic embolism in the inferior vena cava and bilateral iliac veins after PVP. Fortunately, after conservative therapy, we achieved a satisfactory result. Unfortunately, we could not explain the cause of the cement leakage and formation of the thrombotic embolism. We believe that surgeons should have a better understanding of the fracture pattern and anatomy of the vertebral venous net system. They should also perform meticulous imaging monitoring with slower pushing of the cement to minimize the risks during the PVP. This case report highlights a rare, but potentially life-threatening, complication of PVP. Surgeons need to be aware of the possibility of cement leakage and the formation of cement-associated thrombotic embolism so that they are much more vigilant when performing PVP.
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http://dx.doi.org/10.1177/03000605211046115DOI Listing
September 2021

Stable Perovskite Solar Cells with Bulk-Mixed Electron Transport Layer by Multifunctional Defect Passivation.

ACS Appl Mater Interfaces 2021 Sep 13;13(37):44401-44408. Epub 2021 Sep 13.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, People's Republic of China.

Multifarious electron transport layers (ETLs), especially fullerene derivatives, have been applied in organic-inorganic hybrid perovskite (OIHP) devices owing to their superior optoelectronic properties. However, a PCBM Lewis acid molecule can only passivate the iodine-rich defect sites, which cannot solve the problem of uncoordinated Pb and water oxygen erosion due to the high volatility of halide I and the hydrophilicity of organic cation MA. Herein, we introduce a Lewis base, TBA-Azo with an electron-donating Azo moiety, and hydrophobic long alkyl chains into the PCBM layer to form a multifunctional bulk-mixed electron transport layer (MBE). PCBM of MBE can combine with iodine-rich trap sites at the surface and grain boundaries of perovskite. TBA-Azo molecules of MBE can passivate uncoordinated Pb by forming Lewis adducts and isolate water/oxygen at the perovskite surface with hydrophobic alkyl chains. It results in a decrease of trap densities with 1 order of magnitude, effectively inhibiting both bimolecular and trap-induced recombination and thus elongating the carrier lifetime. The passivation of MBE can effectively improve the open-circuit voltage from 1.05 to 1.10 V. Furthermore, three long carbon chain structures of TBA-Azo in MBE can improve the water-resistant ability of OIHP devices, which can maintain 90% of the original PCE after 500 h at the humidity of 50 ± 10%. We believe that the MBE with multifunctional defect passivation provides a strategy for simultaneously achieving high-performance and high-stability OIHP optoelectronic devices.
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http://dx.doi.org/10.1021/acsami.1c12826DOI Listing
September 2021

Selective difunctionalization of electron-deficient alkynes: access to ()-2-iodo-3-(methylthio)acrylate.

Org Biomol Chem 2021 Sep 2. Epub 2021 Sep 2.

College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425100, China.

A convenient and efficient approach to ()-2-iodo-3-(methylthio)acrylate has been developed through direct iodothiomethylation of alkynes with aqueous HI and DMSO under mild conditions. This novel protocol has demonstrated a unique difunctionalization of electron-deficient alkynes with a broad substrate scope and excellent functional-group tolerance. Preliminary mechanistic studies indicated that prior diiodination of alkynes, followed by nucleophilic substitution with generated DMS led to the formation of ()-2-iodo-3-(methylthio)acrylate.
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http://dx.doi.org/10.1039/d1ob01287hDOI Listing
September 2021

Evaluation of focus and deep learning methods for automated image grading and factors influencing image quality in adaptive optics ophthalmoscopy.

Sci Rep 2021 Aug 17;11(1):16641. Epub 2021 Aug 17.

Centre for Ophthalmology and Visual Science (Incorporating Lions Eye Institute), The University of Western Australia, Perth, WA, 6009, Australia.

Adaptive optics flood illumination ophthalmoscopy (AO-FIO) is an established imaging tool in the investigation of retinal diseases. However, the clinical interpretation of AO-FIO images can be challenging due to varied image quality. Therefore, image quality assessment is essential before interpretation. An image assessment tool will also assist further work on improving the image quality, either during acquisition or post processing. In this paper, we describe, validate and compare two automated image quality assessment methods; the energy of Laplacian focus operator (LAPE; not commonly used but easily implemented) and convolutional neural network (CNN; effective but more complex approach). We also evaluate the effects of subject age, axial length, refractive error, fixation stability, disease status and retinal location on AO-FIO image quality. Based on analysis of 10,250 images of 50 × 50 μm size, at 41 retinal locations, from 50 subjects we demonstrate that CNN slightly outperforms LAPE in image quality assessment. CNN achieves accuracy of 89%, whereas LAPE metric achieves 73% and 80% (for a linear regression and random forest multiclass classifier methods, respectively) compared to ground truth. Furthermore, the retinal location, age and disease are factors that can influence the likelihood of poor image quality.
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http://dx.doi.org/10.1038/s41598-021-96068-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371000PMC
August 2021

An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination.

Nat Commun 2021 08 16;12(1):4964. Epub 2021 Aug 16.

Laboratory of Controllable Nanopharmaceuticals, Chinese Academy of Sciences (CAS) Center for Excellence in Nanoscience and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, China.

Immunological adjuvants are essential for successful cancer vaccination. However, traditional adjuvants have some limitations, such as lack of controllability and induction of systemic toxicity, which restrict their broad application. Here, we present a light-activable immunological adjuvant (LIA), which is composed of a hypoxia-responsive amphiphilic dendrimer nanoparticle loaded with chlorin e6. Under irradiation with near-infrared light, the LIA not only induces tumour cell lysis and tumour antigen release, but also promotes the structural transformation of 2-nitroimidazole containing dendrimer to 2-aminoimidazole containing dendrimer which can activate dendritic cells via the Toll-like receptor 7-mediated signaling pathway. The LIA efficiently inhibits both primary and abscopal tumour growth and induces strong antigen-specific immune memory effect to prevent tumour metastasis and recurrence in vivo. Furthermore, LIA localizes the immunological adjuvant effect at the tumour site. We demonstrate this light-activable immunological adjuvant offers a safe and potent platform for in situ cancer vaccination.
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http://dx.doi.org/10.1038/s41467-021-25197-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368031PMC
August 2021

HBx represses WDR77 to enhance HBV replication by DDB1-mediated WDR77 degradation in the liver.

Theranostics 2021 25;11(17):8362-8378. Epub 2021 Jul 25.

Department of Gastrointestinal Cancer Biology, Tianjin Cancer Institute, Liver Cancer Center, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, P.R. China.

Hepatitis B x protein (HBx) is required to initiate and maintain the replication of hepatitis B virus (HBV). Protein arginine methyltransferases 5 (PRMT5) negatively regulates HBV transcription. WD repeat domain 77 protein (WDR77) greatly enhances the methyltransferase activity of PRMT5. However, the role of WDR77 in the modulation of cccDNA transcription and HBV replication is poorly understood. In this study, we investigated the mechanism by which HBx modulated HBV replication involving WDR77 in the liver. A human liver-chimeric mouse model was established. Immunohistochemistry (IHC) staining, Western blot analysis, Southern blot analysis, Northern blot analysis, immunofluorescence assays, ELISA, RT-qPCR, CoIP assays, and ChIP assays were performed in human liver-chimeric mouse model, primary human hepatocytes (PHHs), HepG2-NTCP, dHepaRG and HepG2 cell lines. HBV infection and HBx expression remarkably reduced the protein levels of WDR77 in human liver-chimeric mice and HepG2-NTCP cells. WDR77 restricted cccDNA transcription and HBV replication in PHHs and HepG2-NTCP cells. Mechanically, WDR77 enhanced PRMT5-triggered symmetric dimethylation of arginine 3 on H4 (H4R3me2s) on the cccDNA minichromosome to control cccDNA transcription. HBx drove the cellular DDB1-containing E3 ubiquitin ligase to degrade WDR77 through recruiting WDR77, leading to the disability of methyltransferase activity of PRMT5. Thus, HBx promoted HBV replication by driving a positive feedback loop of HBx-DDB1/WDR77/PRMT5/H4R3me2s/cccDNA/HBV/HBx in the liver. HBx attenuates the WDR77-mediated HBV repression by driving DDB1-induced WDR77 degradation in the liver. Our finding provides new insights into the mechanism by which HBx enhances HBV replication in the liver.
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http://dx.doi.org/10.7150/thno.57531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343998PMC
July 2021

The Relationship Between UBE2C and AGGF1 Overexpression and Tumor Angiogenesis in Non-Small Cell Lung Cancer.

Cancer Manag Res 2021 30;13:5919-5930. Epub 2021 Jul 30.

Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People's Republic of China.

Background: Tumor infiltration and metastasis are the leading causes of death for patients with tumors. Angiogenesis is a prerequisite for tumor growth and metastasis. Angiogenic factor with G patch and FHA domains 1 (AGGF1) is an angiogenic factor, whereas ubiquitin-conjugating enzyme E2C (UBE2C) functions in protein ubiquitination. Microvessel density (MVD) is the most common indicator of tumor microvessels, and vasculogenic mimicry (VM) facilitates blood supply to tumors. This study explored UBE2C and AGGF1 expression in non-small cell lung cancer (NSCLC) and their relationship with angiogenesis and prognosis to identify biological factors that might predict NSCLC infiltration, metastasis, and prognosis.

Methods: The specimens and clinical pathological data of patients with NSCLC confirmed by pathology after surgical resection between January 2013 and December 2015 were collected. UBE2C and AGGF1 expression, as well as microvessel formation and VM in NSCLC, was observed using immunohistochemistry. The relationships between UBE2C, AGGF1, MVD, VM, and clinical pathological parameters and their relationships with overall survival (OS) and disease-free survival (DFS) were analyzed.

Results: UBE2C and AGGF1 levels in NSCLC tissues were significantly higher than those in corresponding normal tissues (57.1% vs 15.6 and 59.7% vs 25.3%, respectively; < 0.05). UBE2C, AGGF1, MVD, and VM were positively correlated with each other ( < 0.05) and were all related to tumor size, lymph node metastasis, and tumor-node-metastasis stage ( < 0.05). Kaplan-Meier analysis showed that patient OS and DFS in the UBE2C, AGGF1, VM-positive, and high-MVD groups were reduced (all < 0.001). Univariate and multivariate analyses showed that UBE2C, AGGF1, VM, and MVD were independent risk factors for NSCLC prognosis.

Conclusion: UBE2C and AGGF1 overexpression is associated with angiogenesis and poor prognosis and may be important for predicting NSCLC invasion, metastasis, and prognosis.
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http://dx.doi.org/10.2147/CMAR.S320393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8331115PMC
July 2021

CS-semi5 Inhibits NF-κB Activation to Block Synovial Inflammation, Cartilage Loss and Bone Erosion Associated With Collagen-Induced Arthritis.

Front Pharmacol 2021 9;12:655101. Epub 2021 Jul 9.

Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease that affects 1% of the population. CS-semi5 is a semisynthetic chondroitin sulfate. In this study, CS-semi5 was shown to have positive effects on a model of collagen-induced arthritis (CIA). CS-semi5 treatment had obvious effects on weight loss and paw swelling in CIA mice. Post-treatment analysis revealed that CS-semi5 alleviated three main pathologies (i.e., synovial inflammation, cartilage erosion and bone loss) in a dose-dependent manner. Further study showed that CS-semi5 could effectively reduce TNF-α and IL-1β production in activated macrophages the NF-κB pathway. CS-semi5 also blocked RANKL-trigged osteoclast differentiation from macrophages. Therefore, CS-semi5 may effectively ameliorate synovial inflammation, cartilage erosion and bone loss in RA through NF-κB deactivation.
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http://dx.doi.org/10.3389/fphar.2021.655101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298759PMC
July 2021

Approaches to tuning the exceptional point of PT-symmetric double ridge stripe lasers.

Opt Express 2021 Jun;29(13):20440-20448

Electrically injected Parity-time (PT)-symmetric double ridge stripe semiconductor lasers lasing at 980 nm range are designed and measured. The spontaneous PT-symmetric breaking point or exceptional point (EP) of the laser is tuned below or above the lasing threshold by means of varying the coupling constant or the mirror loss. The linewidth of the optical spectrum of the PT-symmetric laser is narrowed, compared with that of traditional single ridge (SR) laser and double ridge (DR) laser. Furthermore, the far field pattern of the PT-symmetric laser with EP below the lasing threshold is compared with that of the PT-symmetric laser with EP above the lasing threshold experimentally. It is found that when the laser start to lase, the former is single-lobed while the latter is double-lobed. when the current continues to increase, the former develops into double lobe directly while the latter first develops into single lobe and then double lobe again.
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http://dx.doi.org/10.1364/OE.423266DOI Listing
June 2021

Exploring the multi-dimensional coordination relationship between population urbanization and land urbanization based on the MDCE model: A case study of the Yangtze River Economic Belt, China.

PLoS One 2021 30;16(6):e0253898. Epub 2021 Jun 30.

School of Information and Safety Engineering, Zhongnan University of Economics and Law, Wuhan, Hubei, China.

The rapid development of urbanization has had a dramatic impact on the economy, society and environment in China. In this context, the coordination relationship between population urbanization and land urbanization is essential for achieving sustainable urbanization. Based on the statistical data from 2007-2017 in the Yangtze River Economic Belt (YEB), this paper established the multi-dimensional coordination evaluation (MDCE) model by using the speed coordination evaluation (SCE) model, the level consistency evaluation (LCE) model, the entropy method and the space matching evaluation (SME) model to evaluate the coordination relationship between population urbanization and land urbanization from the speed-level-space perspective. The results showed that from 2007 to 2017: 1) the development speed of population urbanization and land urbanization in the YEB were more and more coordinated, and the speed of population urbanization lagged behind that of land urbanization. In addition, the overall development speed of the 11 provinces declined, and most of them were characterized by excessive development of land urbanization. 2) the development level of population urbanization and land urbanization in the YEB were all high, but the development level of population urbanization was lower than that of land urbanization. Further, the development level of the 11 provinces remained stable and high, and continuously improved. 3) the space matching of population urbanization and land urbanization in the YEB had a high degree of coordination, and the space matching degree of population urbanization was higher than that of land urbanization. Moreover, the space matching of most provinces in the region had declined, but the change was small. Finally, this paper proposes the policy recommendations on the coordinated development of population and land urbanization at the institutional, market and management levels to achieve coordinated and sustainable urbanization.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253898PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244905PMC
June 2021

Association between visit-to-visit variability of glycated albumin and diabetic retinopathy among patients with type 2 diabetes - A prospective cohort study.

J Diabetes Complications 2021 Sep 29;35(9):107971. Epub 2021 May 29.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai 200233, China. Electronic address:

Aim: There is a paucity of studies regarding the association between long-term glycemic variability with the risk of diabetic retinopathy (DR) in patients with type 2 diabetes. Therefore, the purpose of this study is to explore the association of glycated albumin (GA) variability and HbA1c variability with the risk of DR in patients with type 2 diabetes.

Methods: This prospective cohort study included 315 inpatients with type 2 diabetes (191 males and 124 females) with at least 3 measurements of GA and HbA1c within 2years prior to the baseline investigation. Different GA and HbA1c variability markers were calculated, including CV, variability independent of the mean (VIM), and the average real variability (ARV). Cox proportional hazard regression models were used to explore the association between visit-to-visit variability of GA and HbA1c and the risk of DR.

Results: After an average follow-up of 3.42years, 81 patients developed incident DR. Multivariable-adjusted (diabetes duration, smoking status, systolic blood pressure, albumin to creatinine ratio, triglycerides, using fibrates, and mean HbA1c) hazard ratios of DR associated with each unit increase in GA-CV, GA-VIM, and GA-ARV were 1.05 (95% CI 1.02-1.09), 1.69 (95% CI 1.24-2.32), and 1.13 (95%CI 1.04-1.23), respectively. However, there was no significant association between visit-to-visit HbA1c variability and the risk of DR.

Conclusions: The present study indicated that visit-to-visit variability of GA can predict the risk of incident DR in patients with type 2 diabetes, and the prediction ability is independent of the average HbA1c levels.
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http://dx.doi.org/10.1016/j.jdiacomp.2021.107971DOI Listing
September 2021

Saliva 1,5-anhydroglucitol is associated with early-phase insulin secretion in Chinese patients with type 2 diabetes.

BMJ Open Diabetes Res Care 2021 06;9(1)

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, China

Introduction: Saliva collection is a non-invasive test and is convenient. 1,5-anhydroglucitol (1,5-AG) is a new indicator reflecting short-term blood glucose levels. This study aimed to explore the relationship between saliva 1,5-AG and insulin secretion function and insulin sensitivity.

Research Design And Methods: Adult patients with type 2 diabetes who were hospitalized were enrolled. Based on blood glucose and C-peptide, homeostasis model assessment 2 for β cell secretion function, C-peptidogenic index (CGI), △2-hour C-peptide (2hCP)/△2-hour postprandial glucose (2hPG), ratio of 0-30 min area under the curve for C-peptide and area under the curve for glucose (AUC/AUC), and AUC/AUC were calculated to evaluate insulin secretion function, while indicators such as homeostasis model assessment 2 for insulin resistance were used to assess insulin sensitivity.

Results: We included 284 subjects (178 men and 106 women) with type 2 diabetes aged 20-70 years. The saliva 1,5-AG level was 0.133 (0.089-0.204) µg/mL. Spearman's correlation analysis revealed a significantly negative correlation between saliva 1,5-AG and 0, 30, and 120 min blood glucose, glycated hemoglobin A, and glycated albumin (all p<0.05), and a significantly positive association between saliva 1,5-AG and CGI (=0.171, p=0.004) and AUC /AUC (=0.174, p=0.003). The above correlations still existed after adjusting for age, sex, body mass index, and diabetes duration. In multiple linear regression, saliva 1,5-AG was an independent factor of CGI (standardized =0.135, p=0.015) and AUC /AUC (standardized =0.110, p=0.020).

Conclusions: Saliva 1,5-AG was related to CGI and AUC/AUC in patients with type 2 diabetes.

Trial Registration Number: ChiCTR-SOC-17011356.
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http://dx.doi.org/10.1136/bmjdrc-2021-002199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231033PMC
June 2021

Retinal Differential Light Sensitivity Variation Across the Macula in Healthy Subjects: Importance of Cone Separation and Loci Eccentricity.

Transl Vis Sci Technol 2021 05;10(6):16

Centre for Ophthalmology and Visual Science (Incorporating Lions Eye Institute), The University of Western Australia, Nedlands, Western Australia, Australia.

Purpose: Microperimetry measures differential light sensitivity (DLS) at specific retinal locations. The aim of this study is to examine the variation in DLS across the macula and the contribution to this variation of cone distribution metrics and retinal eccentricity.

Methods: Forty healthy eyes of 40 subjects were examined by microperimetry (MAIA) and adaptive optics imaging (rtx1). Retinal DLS was measured using the grid patterns: foveal (2°-3°), macular (3°-7°), and meridional (2°-8° on horizontal and vertical meridians). Cone density (CD), distribution regularity, and intercone distance (ICD) were calculated at the respective test loci coordinates. Linear mixed-effects regression was used to examine the association between cone distribution metrics and loci eccentricity, and retinal DLS.

Results: An eccentricity-dependent reduction in DLS was observed on all MAIA grids, which was greatest at the foveal-parafoveal junction (2°-3°) (-0.58 dB per degree, 95% confidence interval [CI]; -0.91 to -0.24 dB, P < 0.01). Retinal DLS across the meridional grid changed significantly with each 1000 cells/deg2 change in CD (0.85 dB, 95% CI; 0.10 to 1.61 dB, P = 0.03), but not with each arcmin change in ICD (1.36 dB, 95% CI; -2.93 to 0.20 dB, P = 0.09).

Conclusions: We demonstrate significant variation in DLS across the macula. Topographical change in cone separation is an important determinant of the variation in DLS at the foveal-parafoveal junction. We caution the extrapolation of changes in DLS measurements to cone distribution because the relationship between these variables is complex.

Translational Relevance: Cone density is an independent determinant of DLS in the foveal-parafoveal junction in healthy eyes.
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http://dx.doi.org/10.1167/tvst.10.6.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114004PMC
May 2021

Thermally treated zeolitic imidazolate framework-8 (ZIF-8) for visible light photocatalytic degradation of gaseous formaldehyde.

Chem Sci 2020 May 21;11(26):6670-6681. Epub 2020 May 21.

School of Energy and Environment, City University of Hong Kong Tat Chee Avenue Kowloon Hong Kong China +852 3442 0688 +852 3442 7714.

The development of wide-spectrum responsive photocatalysts for efficient formaldehyde (HCHO) removal is highly desired yet remains a great challenge. Here we successfully converted zeolitic imidazolate framework-8 (ZIF-8), one of the most well-studied metal-organic frameworks (MOFs), from routine ultraviolet-driven to novel broad-spectrum-driven photocatalyst a facile thermal treatment. The isocyanate groups (-N[double bond, length as m-dash]C[double bond, length as m-dash]O) formed in the thermally treated ZIF-8 (ZIF-8-T) is crucial in enabling the superior photocatalytic performance in formaldehyde degradation. Specifically, the best-performing ZIF-8-T sample showed around 2.1 and 9.4 times the HCHO adsorption amount and the solar photocatalytic degradation rate, respectively, of pristine ZIF-8. In addition, ZIF-8-T exhibited visible light ( ≥ 400 nm) photocatalytic HCHO degradation performance, photo-converting 72% and nearly 100% of 20 ppm and 10 ppm HCHO within 1 hour, respectively. This work affords new insights and knowledge that inspire and inform the design and development of MOF-based photocatalysts with broad-spectrum responses for efficient air purification operations.
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http://dx.doi.org/10.1039/d0sc01397hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159372PMC
May 2020

Association Between Neck Circumference and Microalbuminuria in Community Residents.

Diabetes Metab Syndr Obes 2021 28;14:2407-2414. Epub 2021 May 28.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital; Shanghai Clinical Center for Diabetes; Shanghai Key Clinical Center for Metabolic Disease; Shanghai Diabetes Institute; Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, People's Republic of China.

Objective: Neck circumference (NC) represents the subcutaneous fat deposition in the neck and is an effective indicator for evaluating metabolic disorders, such as metabolic syndrome, subclinical atherosclerosis, and non-alcoholic fatty liver disease. Microalbuminuria (MAU) is regarded as a potential sign of systemic endothelial dysfunction and microvascular abnormalities. The aim of this study was to elucidate the association of NC with urine albumin-to-creatinine ratio (UACR) and MAU.

Methods: A total of 1882 Shanghai community residents were enrolled (816 men and 1066 women), with age ranging from 40 to 80 years. Anthropometric parameters, including NC, and biochemical indices were measured. MAU was determined if 30 mg/g ≤ UACR < 300 mg/g. An elevated NC was defined as NC ≥ 38.5 cm for men and NC ≥ 34.5 cm for women.

Results: Individuals with an elevated NC had significantly higher prevalence of MAU and UACR values than those with normal NC in both men and women (all < 0.05). The logistic regression analysis showed that there were significant and positive associations between elevated NC and the increasing risk of MAU after adjusting for lipid profile and glycemic indices ( = 0.007 for men and = 0.009 for women). After further adjusting for blood pressure, elevated NC caused an 69.3% additional risk of MAU in men ( = 0.037) and the positive correlation in women disappeared ( = 0.131).

Conclusion: There was an independent and positive association between elevated NC and the risk of MAU in men in the Chinese community population.

Chinese Clinical Trial Registry Wwwchictrorgcn Registration Number: ChiCTR1900024011.
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http://dx.doi.org/10.2147/DMSO.S313202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169818PMC
May 2021

Phthalates and Their Impacts on Human Health.

Healthcare (Basel) 2021 May 18;9(5). Epub 2021 May 18.

College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.

Phthalates are a series of widely used chemicals that demonstrate to be endocrine disruptors and are detrimental to human health. Phthalates can be found in most products that have contact with plastics during producing, packaging, or delivering. Despite the short half-lives in tissues, chronic exposure to phthalates will adversely influence the endocrine system and functioning of multiple organs, which has negative long-term impacts on the success of pregnancy, child growth and development, and reproductive systems in both young children and adolescents. Several countries have established restrictions and regulations on some types of phthalates; however, we think that more countries should establish constraints or substitute measures for phthalates to reduce health risks. This article aims to summarize the adverse impacts of phthalates on human health, analyze the toxicity mechanism, assess the risks, and finally provide feasible strategies to reduce exposure of the public to phthalates.
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http://dx.doi.org/10.3390/healthcare9050603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157593PMC
May 2021

Preparation of Ultrafiltration Membrane by Polyethylene Glycol Non-Covalent Functionalized Multi-Walled Carbon Nanotubes: Application for HA Removal and Fouling Control.

Membranes (Basel) 2021 May 17;11(5). Epub 2021 May 17.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Pingleyuan No.100, Beijing 100124, China.

Polyethylene glycol (PEG) non-covalent-functionalized multi-walled carbon nanotubes (MWCNT) membrane were prepared by vacuum filtration. The dispersion and stability of MWCNT non-covalent functionalized with PEG were all improved. TEM characterization and XPS quantitative analysis proved that the use of PEG to non-covalent functionalize MWCNT was successful. SEM image analysis confirmed that the pore size of PEG-MWCNT membrane was more concentrated and distributed in a narrower range of diameter. Contact angle measurement demonstrated that PEG non-covalent functionalization greatly enhanced the hydrophilicity of MWCNT membranes. The results of pure water flux showed that the PEG-MWCNT membranes could be categorized into low pressure membrane. PEG-MWCNT membrane had a better effect on the removal of humic acid (HA) and a lower TMP growth rate compared with a commercial 0.01-μm PVDF ultrafiltration membrane. During the filtration of bovine serum albumin (BSA), the antifouling ability of PEG-MWCNT membranes were obviously better than the raw MWCNT membranes. The TMP recovery rate of PEG-MWCNT membrane after cross flushing was 79.4%, while that of raw MWCNT- and MWCNT membrane were only 14.9% and 28.3%, respectively. PEG non-covalent functionalization improved the antifouling ability of the raw MWCNT membranes and reduced the irreversible fouling, which effectively prolonged the service life of MWCNT membrane.
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http://dx.doi.org/10.3390/membranes11050362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156076PMC
May 2021

A core-shell structured COVID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity.

Signal Transduct Target Ther 2021 05 31;6(1):213. Epub 2021 May 31.

NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Although inoculation of COVID-19 vaccines has rolled out globally, there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries. Here, we report on the development of a highly efficacious mRNA vaccine, SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex (LPP) nanoparticles. SW0123 is easy to produce using a large-scale microfluidics-based apparatus. The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability, and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration. Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123, represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2, but also a panel of variants including D614G and N501Y variants. In addition, SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge. Taken together, SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans.
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http://dx.doi.org/10.1038/s41392-021-00634-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165147PMC
May 2021

Association of advanced glycation end products with diabetic retinopathy in type 2 diabetes mellitus.

Diabetes Res Clin Pract 2021 Jul 29;177:108880. Epub 2021 May 29.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes; Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai 200233, China. Electronic address:

Aims: Advanced glycation end products (AGEs) were reported to be associated with diabetes development and diabetes related complications when accumulated in high levels. This study investigated the association between AGEs and diabetic retinopathy (DR).

Methods: A total of 1,471 patients with type 2 diabetes were enrolled. Fundus radiography was used for DR measurement. AGEs were detected through non-invasive skin autofluorescence method.

Results: Patients with more advanced DR showed a much higher AGEs, and the prevalence of DR (based on the severity) increase with ascending AGEs quartiles (all P for trend < 0.001). The multivariable-adjusted odds ratios of any DR across AGEs quartiles were 1.00, 1.69 (95% confidence interval [CI] 1.16-2.47), 1.58 (95%CI 1.06-2.37) and 1.60 (95%CI 1.05-2.44) (P for trend = 0.044), respectively. Similar results were found in vision-threatening DR (VTDR) subgroup (P for trend = 0.009). When AGEs was considered as a continuous variable by using restricted cubic splines, a graded positive association of AGEs with the odds of any presence of DR was observed (P for trend < 0.001). Further, we found that AGEs presented the similar predictive value for any DR with glycated hemoglobin A (HbA). When it comes to VTDR, AGEs showed a significantly higher efficacy in early screening than HbA (P = 0.002). With a cut-off point of 77.1, the sensitivity, specificity and area under the curve of AGEs were 90.0% (95%CI 76.3-97.2%), 49.4% (95%CI 46.8-52.0%), and 0.728 (95%CI 0.704-0.750), respectively.

Conclusion: Non-invasive measured skin AGEs, associated with the prevalence of all stages of DR, might be a more suitable indicator than HbA for mimicking the poor prognosis of hyperglycemia.
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http://dx.doi.org/10.1016/j.diabres.2021.108880DOI Listing
July 2021

External treatment of traditional Chinese medicine for radiation enteritis: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(21):e26014

The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: Radiation enteritis (RE) is a common complication that often occurs after radiotherapy for abdominal and pelvic malignancies. RE could influence patients' quality of life seriously and it is difficult to cure by conventional treatments. A lot of studies have revealed that the external treatment of traditional Chinese medicine (TCM) for RE is a safe and economical approach, but there is no relevant systematic review. The present study performed a systematic review and meta-analysis to compare TCM external treatment and conventional treatment for RE to evaluate the effectiveness and safety of external treatment of traditional Chinese medicine in the treatment of RE.

Methods: Cochrane Library, PubMed, Embase, China National Knowledge Infrastructure (CNKI), Wan-Fang database, VIP Chinese Science and Technique Journals Database, and the Chinese Biomedical Literature Database (CBM) were searched. The time of publication was limited from inception to April, 2021. Two reviewers independently searched for the selected articles and extract the data. The RevMan V.5.3 statistical software (Cochrane Collaboration) and Stata V.16.0 software were used to conduct the meta-analysis.

Results: We will show the results of this study in a peer-reviewed journal.

Conclusion: This meta-analysis will provide reliable evidence for external treatment of TCM in the treatment of RE.

Inplasy Registration Number: INPLASY202140120.
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http://dx.doi.org/10.1097/MD.0000000000026014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154505PMC
May 2021

Paired-like homeodomain transcription factor 2 affects endometrial cell function and embryo implantation through the Wnt/β-catenin pathway.

Cell Biol Int 2021 Sep 31;45(9):1957-1965. Epub 2021 May 31.

Core Laboratory Glycobiology & Glycoengineering, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, China.

The successful implantation of embryos is crucial for pregnancy in mammals. This complex process is inevitably dependent on the development of the endometrium. The paired-like homeodomain transcription factor 2 (PITX2) is involved in a variety of biological processes, but whether it is involved in embryo implantation has not been reported. In this study, we aimed to investigate uterine expression and regulation of PITX2 during implantation. We found that PITX2 was elevated in the human endometrium in the secretory phase. The results of the pregnant mouse models showed that PITX2 expression was spatiotemporal in mouse endometrial tissue throughout peri-implantation period, and it was significantly upregulated at the time of implantation. Interestingly, PITX2 was mainly localized to the glandular epithelium cells on D2.5-3.5 of pregnancy, while D5.5-6.5 was largely expressed in stromal cells. In vitro, PITX2 regulated endometrial cells proliferation, migration, invasion, and other functions through the Wnt/β-catenin signaling pathway. In addition, a significant decrease in the rate of embryo implantation was observed after injecting PITX2 small interfering RNA into the uterine horn. These results demonstrate the effects of PITX2 on the physiological function of endometrial cells and embryo implantation, suggesting a role in the endometrial regulatory mechanism during implantation.
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http://dx.doi.org/10.1002/cbin.11636DOI Listing
September 2021

Effect of physicochemical properties on fate of nanoparticle-based cancer immunotherapies.

Acta Pharm Sin B 2021 Apr 9;11(4):886-902. Epub 2021 Mar 9.

Laboratory of Controllable Nanopharmaceuticals, Chinese Academy of Sciences (CAS) Center for Excellence in Nanoscience and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.

Current advances of immunotherapy have greatly changed the way of cancer treatment. At the same time, a great number of nanoparticle-based cancer immunotherapies (NBCIs) have also been explored to elicit potent immune responses against tumors. However, few NBCIs are nearly in the clinical trial which is mainly ascribed to a lack understanding of fate of nanoparticles (NPs) for cancer immunotherapy. NPs for cancer immunotherapy mainly target the immune organs or immune cells to enable efficient antitumor immune responses. The physicochemical properties of NPs including size, shape, elasticity and surface properties directly affect their interaction with immune systems as well as their fate and therapeutic effect. Hence, systematic analysis of the physicochemical properties and their effect on fate is urgently needed. In this review, we first recapitulate the fundamentals for the fate of NBCIs including physio-anatomical features of lymphatic system and strategies to modulate immune responses. Moreover, we highlight the effect of physicochemical properties on their fate including lymph nodes (LNs) drainage, cellular uptake and intracellular transfer. Challenges and opportunities for rational design of NPs for cancer immunotherapy are also discussed in detail.
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http://dx.doi.org/10.1016/j.apsb.2021.03.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105773PMC
April 2021

Online monitoring of air activation at the China spallation neutron source.

J Environ Radioact 2021 Aug 11;234:106642. Epub 2021 May 11.

Institute of High Energy Physics (IHEP), Chinese Academy of Sciences (CAS), Beijing, 100049, China; Spallation Neutron Source Science Center, Dongguan, 523808, China.

During the operation of high-energy proton accelerators, the air in the tunnel is activated with the production of radionuclides. For CSNS (China Spallation Neutron Source), the first pulsed neutron source in China for multidisciplinary research, an online air activation monitoring system was developed to evaluate the radiation safety of the staff and the public, which consisted of a NaI detector, Pb shielding, an MB container and a control system. With the monitoring system, gamma spectra of the activated air from controlled areas are measured, and the activity concentration and immersion dose rates of radionuclides in air are calculated and displayed in real time. The system has been in stable operation since February 2020, and results have been obtained for the evaluation of the radiation risk from activated air.
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http://dx.doi.org/10.1016/j.jenvrad.2021.106642DOI Listing
August 2021

No Association between Neck Circumference and Free Triiodothyronine in Euthyroid Men.

Int J Endocrinol 2021 19;2021:5570193. Epub 2021 Apr 19.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai 200233, China.

Objective: Neck circumference (NC) is a simple anthropometric index for the assessment of upper body obesity. Thyroid hormones are closely related to obesity, body fat distribution indicators, and metabolic parameters. However, there are currently no reports on the association between NC and thyroid hormones in the Chinese population. This study aimed to explore the relationship between NC and thyroid hormones in men with normal thyroid function.

Methods: A total of 737 euthyroid men from Shanghai communities were enrolled. Anthropometric parameters, including NC and waist circumference (WC), were measured. Serum thyroid hormones were measured by electrochemical luminescence immunoassay.

Results: NC, WC, and body mass index (BMI) were significantly positively correlated with serum free triiodothyronine (FT3) (all < 0.05). FT3 levels all presented significant upward trends with the increase in NC, WC, or BMI quartiles (all for trend < 0.05), whereas there were no significant correlations between the three obesity indices and free thyroxine or thyroid-stimulating hormone (both > 0.05). After adjustment for metabolic confounding factors such as age, blood pressure, blood glucose, lipid profiles, and CRP in multiple linear regression analysis, the correlation between FT3 and NC disappeared (standardized  = -0.015, =0.705), and FT3 remained significantly associated with WC (standardized  = 0.103, =0.012) and BMI (standardized  = 0.082, =0.047).

Conclusions: In euthyroid men from Shanghai communities, there was no independent correlation between serum FT3 levels and NC. The trial was registered with ChiCTR1900024011.
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http://dx.doi.org/10.1155/2021/5570193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079210PMC
April 2021

Near-infrared inorganic nanomaterial-based nanosystems for photothermal therapy.

Nanoscale 2021 May;13(19):8751-8772

College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, P. R. China.

The development of robust materials for treating diseases through non-invasive photothermal therapy (PTT) has attracted increasing attention in recent years. Among various types of nanomaterials, inorganic nanomaterials with strong absorption in the near-infrared (NIR) window can be employed as high-efficiency photothermal agents to treat cancer and bacterial infections. In addition, inorganic nanomaterials can be easily combined with other drugs or chemical reagents to construct multifunctional nanomaterials to cascade stimulation responses, enhance therapeutic effects, and perform precise medical treatments. In this review, focusing on the latest developments of inorganic nanomaterials in photothermal therapy, we firstly introduced the light-to-heat conversion mechanism of inorganic nanomaterials. Secondly, we summarized the application of common inorganic nanomaterials, such as metallic nanoparticles, transition metal oxide nanoparticles and two dimensional (2D) nanosheets. In addition, the strategy of developing multifunctional nano-platforms with excellent biocompatibility as well as good targeted capability was also expounded. Finally, challenges and new perspectives for designing effective inorganic nanomaterial-based nanosystems for photothermal assisted therapy were also discussed.
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http://dx.doi.org/10.1039/d1nr00323bDOI Listing
May 2021

Up-regulating TIPE2 alleviates inflammatory pain by suppressing microglial activation-mediated inflammatory response via inhibiting Rac1/NF-κB pathway.

Exp Cell Res 2021 07 30;404(1):112631. Epub 2021 Apr 30.

Department of Pain Clinic, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, 264100, China.

TNF-α-inducible protein 8-like 2 (TIPE2) is a recently discovered regulator of inflammation that can maintain immune homeostasis, exerting a significant role in the development of inflammation-related diseases. Here, we aimed to explore the role and potential regulatory mechanism of TIPE2 in the progression of inflammatory pain. In the present study, a mouse BV2 microglia cell activation-mediated inflammatory model was developed with LPS induction, and a mouse inflammatory pain model was established with complete Freund's adjuvant (CFA) injection. In vitro, the TIPE2 expression was decreased in LPS-induced BV2 cells. Overexpression of TIPE2 mitigated LPS-medicated microglial activation via decreasing nitric oxide (NO) generation and the expression of microglia marker IBA-1. Notably, increasing TIPE2 expression alleviated microglial activation-triggered expression levels and releases of proinflammatory factors such as TNF-α, IL-1β, and IL-6. Mechanism analysis verified that overexpression of TIPE2 blunted Rac1-mediated activation of NF-κB pathway following LPS stimulation. More importantly, CFA injection reduced the expression of TIPE2 in a mouse inflammatory pain model and overexpression of TIPE2 alleviated CFA-mediated pain hypersensitivity and inflammatory response, and inactivated microglia cell in vivo. Furthermore, overexpression of TIPE2 decreased Rac1 expression and suppressed the activation of NF-κB pathway in spinal cord after CFA injection. In summary, the present study revealed that overexpression of TIPE2 mitigated inflammatory pain through suppressing microglial activation-induced inflammation by inactivating Rac1/NF-κB pathway. The study provides a novel theoretical foundation for the therapy of inflammatory pain.
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http://dx.doi.org/10.1016/j.yexcr.2021.112631DOI Listing
July 2021

The nucleolus functions as the compartment for histone H2B protein degradation.

iScience 2021 Apr 2;24(4):102256. Epub 2021 Mar 2.

Laboratory of Molecular and Cellular Biology, School of Forensic Sciences, Xi'an Jiao Tong University Health Science Center, Xi'an, Shaanxi 710061, PR China.

Histones are main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. However, how histone protein levels are regulated, especially whether and how histones are degraded, is largely unclear. Here, we found that histone H2B is mainly degraded through the proteasome-mediated pathway, and the lysine-120 site of H2B is essential for its K48-linked polyubiquitination and degradation. Moreover, the degradation-impaired H2BK120R mutant shows an increased nucleolus localization, and inhibition of the proteasome results in an elevated nucleolus distribution of wild-type H2B, which is similar to that of H2BK120R mutants. More importantly, the nucleolus fractions can ubiquitinate and degrade the purified H2B , suggesting that the nucleolus, in addition to its canonical roles regulating ribosome genesis and protein translation, likely associates with H2B degradation. Therefore, these findings revealed a novel mechanism for the regulation of H2B degradation in which a nucleolus-associated proteasome pathway is involved.
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http://dx.doi.org/10.1016/j.isci.2021.102256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995529PMC
April 2021

Increased Serum GDF15 Related to Improvement in Metabolism by Lifestyle Intervention Among Young Overweight and Obese Adults.

Diabetes Metab Syndr Obes 2021 17;14:1195-1202. Epub 2021 Mar 17.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital; Shanghai Clinical Center for Diabetes; Shanghai Diabetes Institute; Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, People's Republic of China.

Aim: Growth differentiation factor 15 (GDF15) is closely related to obesity. This study aimed to explore the influence of weight loss intervention on serum GDF15 levels and the relationship between GDF15 and metabolism.

Materials And Methods: Forty-four overweight and obese adults either adopted a low-carbohydrate diet or performed moderate-to-vigorous exercise for 3 weeks. Weekly follow-ups were conducted to measure body composition, glucose and lipid metabolism and serum GDF15 levels.

Results: After 3 weeks of intervention, body weight, fat mass and waist circumference in both groups decreased ( < 0.05). No significant change in GDF15 levels was observed in the two groups. Among all the subjects, 10 of them (22.7%, group of Down) showed a decrease in GDF15 and 34 (77.3%, group of Up) showed an increase in GDF15 after the intervention. We found that GDF15 increased significantly with the follow-up time in the UP group and waist circumference, fasting serum insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR) and triglycerides (TG) decreased ( for trend <0.05). However, no significant differences in all parameters were detected in Down group. In addition, GDF15 was elevated between 30 mins and 120 mins in a 75-g oral glucose tolerance test. Its trend was negatively correlated with plasma glucose and serum insulin.

Conclusion: Increased serum GDF15 was associated with improvement in metabolism by lifestyle intervention among young overweight and obese adults. The increase of GDF15 could be an indicator to evaluate metabolic improvements in overweight and obese people.

Clinical Trial Registration: www.chictr.org.cn, registration number ChiCTR1800016786.
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http://dx.doi.org/10.2147/DMSO.S302033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982551PMC
March 2021

Comment on " Mannosylated Nanotrinity-Mediated Macrophage Remodeling Combats Infection".

ACS Nano 2021 03;15(3):3541-3543

Department of Oral Implantology & State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

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http://dx.doi.org/10.1021/acsnano.0c07851DOI Listing
March 2021

2D Co-MOF nanosheet-based nanozyme with ultrahigh peroxidase catalytic activity for detection of biomolecules in human serum samples.

Mikrochim Acta 2021 03 19;188(4):130. Epub 2021 Mar 19.

College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou, 450001, China.

A two-dimensional (2D) Co-MOF nanosheet-based nanozyme was developed for colorimetric detection of disease-related biomolecules. The prepared 2D Co-MOFs exhibited ultrahigh peroxidase catalytic activity. 2D Co-MOFs can catalyze the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to the blue product oxTMB, accompanying an obvious change of absorption value at 652 nm. However, alkaline phosphatase can catalyze the hydrolysis of L-ascorbic acid-2-phosphate to produce ascorbic acid which can reduce the oxTMB to TMB, resulting in an obvious color fading. Therefore, by recording the change of absorption value at 652 nm, the 2D Co-MOF nanosheets were used to detect ascorbic acid (AA) and alkaline phosphatase (ALP). The limit of detection for AA and ALP was 0.47 μM and 0.33 U L, respectively. The limit of quantification for AA and ALP was 1.56 μM and 1.1 U L, respectively. The developed nanozyme was successfully used to determine alkaline phosphatase in clinical human serum samples and the results were consistent with those provided by the hospital. Furthermore, by integrating 2D Co-MOF nanosheets with image recognition and data processing function fixed on a smartphone, a portable test of ascorbic acid was reached. Schematic presentation of the preparation of two-dimensional Co-MOF nanosheet-based nanozyme and their application in portable detection of biomolecules.
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http://dx.doi.org/10.1007/s00604-021-04785-2DOI Listing
March 2021
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