Publications by authors named "Yufei Liu"

150 Publications

Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres.

ACS Omega 2021 Apr 30;6(14):9828-9833. Epub 2021 Mar 30.

Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China.

Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing facilities are limited. Polystyrene nanospheres (PSs) with catalytic and specific recognition functions were successfully developed by simultaneously modifying catalase and goat anti-hepatitis B surface antibodies on nanospheres. The modified PSs contributed significantly to the amplification of the signal. Via the specific antigen-antibody reaction, the bifunctional nanospheres could be captured on microplate and then catalyzed the decomposition of hydrogen peroxide to reduce chloroauric acid and synthesize gold nanoparticles (AuNPs). Due to the surface plasmon resonance of AuNPs, the solution color change could be observed with the naked eye and the limit of detection (LOD) was 0.1 ng/mL. Furthermore, the LOD observed with instrumentation was 0.01 ng/mL, which meant that a rapid, efficient, and highly sensitive method for the detection of hepatitis B surface antigens was successfully developed, and neither complex sample pretreatment nor expensive equipment was needed.
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http://dx.doi.org/10.1021/acsomega.1c00507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047666PMC
April 2021

One-step synthesis of fluorescence-enhanced carbon dots for Fe (III) on-off-on sensing, bioimaging and light-emitting devices.

Nanotechnology 2021 Apr 22;32(28). Epub 2021 Apr 22.

Key Laboratory of Optoelectronic Technology & Systems, Chongqing University, Ministry of Education, Chongqing 400044, People's Republic of China.

Carbon quantum dots (CDs), as one of the most potential fluorescent sensing materials, have attracted lots of attention in recent years. However, the low quantum yields, complicated separation and purification procedures have limited its application and large-scale production. In this paper, a facile and universal method was successfully developed to synthesize CDs-silica (SiO) spheres composites (CS composites), which not only demonstrates 10-fold fluorescence enhancement compared with single CDs but also enables the purification with a simple centrifugation step. Meanwhile, fabricated composites also presented superior photoluminescence (PL) stability and high PL intensity, even in some tough environments such as acid/alkali aqueous solution, high/low temperature, and high ion concentration aqueous solution. This simple, fast, low-cost, and efficient synthesis method would potentially expand the application of CDs for clinical analysis, optical sensing (ferric ion (Fe) and pyrophosphate), bioimaging and light-emitting diodes (LEDs).
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http://dx.doi.org/10.1088/1361-6528/abf59bDOI Listing
April 2021

Prognosis of severe lymphopenia after postoperative radiotherapy in non-small cell lung cancer: Results of a long-term follow up study.

Clin Transl Radiat Oncol 2021 May 12;28:54-61. Epub 2021 Mar 12.

Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Purpose: To investigate the incidence and prognosis of severe radiation-induced lymphopenia (sRIL) after postoperative radiotherapy (PORT) for resected NSCLC.

Patients And Methods: Between 1998 and 2017, 170 patients treated with PORT for NSCLC were retrospectively reviewed. Lymphopenia was divided into tertiles with severe lymphopenia defined as absolute lymphocyte counts (ALC) < 0.37 × 10/ul.

Results: sRIL was observed in 32.3% of patients. Multivariable logistic regression analysis indicated sRIL was associated with planning target volume radiation fraction numbers (OR 1.09,  = 0.005) and total lung mean dose (OR 1.12,  = 0.006). With a median follow-up time of 12.2 years, the median progression-free survival and overall survival were 14.8 months and 28.4 months respectively in patients with sRIL, vs. 21.7 months ( = 0.008) and 48.3 months ( = 0.01) respectively in patients without sRIL. Multivariable analyses indicated sRIL significantly decreased OS (HR 1.95,  < 0.01). Since PORT for stage I-II NSCLC was done largely for positive margins, which may confound the contribution of severe RIL, we analyzed stage III separately and found that sRIL also significantly decreased OS (HR 1.88,  = 0.004) in multivariable analysis.

Conclusion: For this long-term outcome study, severe RIL correlated with total lung mean dose and radiation fractionation numbers, and was a strong prognostic factor for poor survival in PORT patients, particularly in patients with stage III NSCLC, highlighting the importance of an intact immune system for post-radiation immunologic disease surveillance.
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http://dx.doi.org/10.1016/j.ctro.2021.02.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985216PMC
May 2021

Poly (γ-glutamic acid)/chitooligo-saccharide/papain hydrogel prevents hypertrophic scar during skin wound healing.

J Biomed Mater Res B Appl Biomater 2021 Mar 19. Epub 2021 Mar 19.

Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.

Hypertrophic scar, a common skin disorder typically caused by deep burns or scald were usually treated via surgical resection, laser irradiation, and drugs. However, all the approaches were always companied with complications and devastatingly subjected to relapse, which indicated the urgently need of an effective treatment method. In this project, a new hydrogel composed of Poly (γ-glutamic acid) (γ-PGA), Chitooligo-saccharide, and Papain was developed via crosslinker (EDC&NHS), and characterized with good porously three-dimensional network structure, good water absorption, and mechanical properties. Besides, G/C/P hydrogel facilitated cell adhesion and inhibited excessive proliferation of fibroblasts, which indicated the potential of in vivo application. After applied onto skin wound healing in vivo on a rabbit ear skin wound model, G/C/P hydrogel inhibited excessive collagen deposition and the generation of hyperplastic scars effectively during wound healing. The hydrogel described here provide a new platform for regeneration field and hold great promise for solving serious skin disorder.
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http://dx.doi.org/10.1002/jbm.b.34830DOI Listing
March 2021

A fluorometric and colorimetric dual-signal nanoplatform for ultrasensitive visual monitoring of the activity of alkaline phosphatase.

J Mater Chem B 2021 Apr 26;9(13):2998-3004. Epub 2021 Feb 26.

Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China.

Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p-aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL. Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.
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http://dx.doi.org/10.1039/d0tb02531cDOI Listing
April 2021

Targets and Mechanism Used by Cinnamaldehyde, the Main Active Ingredient in Cinnamon, in the Treatment of Breast Cancer.

Front Pharmacol 2020 9;11:582719. Epub 2020 Dec 9.

Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Breast cancer has become one of the most common malignant tumors in women owing to its increasing incidence each year. Clinical studies have shown that (L.) (cinnamon) has a positive influence on the prevention and treatment of breast cancer. We aimed to screen the potential targets of cinnamon in the treatment of breast cancer through network pharmacology and explore its potential therapeutic mechanism through cell experiments. We used the TCMSP, TCM Database @ Taiwan, and TCMID websites and established the active ingredient and target database of cinnamon. Thereafter, we used the GeneCards and OMIM databases to establish a breast cancer-related target database, which matched the cinnamon target database. Based on the matching results, the STRING database was used to analyze the interaction between the targets, and the biological information annotation database was used to analyze the biological process of the target (gene ontology) and the pathway enrichment of Kyoto Encyclopedia of Genes and Genomes (KEGG). After establishing the layout of the analysis, we used Cytoscape 3.6.0 software for network analysis. Finally, the cell experiment was used to verify the anti-breast cancer effect of cinnamaldehyde. Our research showed that the main components of cinnamon, including cinnamaldehyde, can play a role in the treatment of breast cancer through 59 possible important targets. Subsequently, enrichment analysis by gene ontology and Kyoto Encyclopedia of Genes and Genomes showed that 83 cell biological processes and 37 pathways were associated with breast cancer ( < 0.05), including the peroxisome proliferator-activated receptor and PI3K-Akt pathway, which are closely related to tumor cell apoptosis. cell verification experiments showed that cinnamaldehyde can significantly inhibit cell proliferation, change cell morphology, inhibit cell migration and invasion ability, and promote cell apoptosis. Our results showed that cinnamaldehyde is a potential novel drug for the treatment and prevention of breast cancer.
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http://dx.doi.org/10.3389/fphar.2020.582719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848847PMC
December 2020

Water-stable perovskite-on-polymer fluorescent microspheres for simultaneous monitoring of pH, urea, and urease.

Anal Bioanal Chem 2021 Mar 19;413(6):1739-1747. Epub 2021 Jan 19.

Key Laboratory of Optoelectronic Technology & Systems, Chongqing University, Ministry of Education, Chongqing, 400044, China.

Perovskite materials have attracted attention due to their excellent optical and electrical properties; however, their unsatisfactory stability limits their application in biochemical detection. In this paper, CsPbBr perovskite quantum dots were successfully encapsulated in poly(styrene/acrylamide) microspheres, using a swelling-shrinking method. The manufactured perovskite microspheres (PDPS composites) not only maintained strong photoluminescence (PL) stability but also demonstrated great water solubility. Additionally, a real-time pH monitoring platform was constructed based on the prepared PDPS composites and dopamine, and the system showed a good linear relationship in a pH range of 4-12. Furthermore, urea could be hydrolyzed to produce hydroxyl groups, thereby increasing the pH of the solution. Therefore, this system was then extended for urea and urease detection. As a result, the detection limits of urea and urease were recorded as 1.67 μM and 2.1 mU/mL, respectively. This development provides an interesting demonstration of the expanding list of applications of perovskite materials.
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http://dx.doi.org/10.1007/s00216-020-03144-zDOI Listing
March 2021

Comparison of quantum dot immunofluorescence histochemistry with conventional immunohistochemistry in detecting Helicobacter pylori infection in paraffin-embedded tissues of gastric biopsy.

J Mol Histol 2021 Jan 8. Epub 2021 Jan 8.

The First College of Clinical Medical Science, China Three Gorges University, Yichang, 443000, China.

Quantum dots (QDs) are a new type of fluorescent label, which has been widely used in many biological and biomedical imaging applications. In this study, we used QDs-based immunofluorescence histochemistry (QDs-IHC) and conventional immunohistochemistry (IHC) techniques to perform a retrospective analysis on paraffin-embedded tissues of gastric biopsies in 203 patients (112 of which were HP positive and 91 were negative). The ability of QDs-IHC to detect Helicobacter pylori (HP) in gastric biopsies compared to IHC technology was evaluated. In our study, both methods showed consistent HP morphology and localization. The positive detection rate of HP for QDs-IHC in formalin-fixed and paraffin-embedded (FFPE) tissue was 54.7% (111/203), and the sensitivity and specificity reached 99.11% and 100%, respectively. However the positive detection rate of HP for IHC was 53.7% (109/203), with a sensitivity and specificity of 97.32% and 100%, respectively. Weak positives (1+) were detected in 2 case of QDs-IHC with negative in IHC, and moderate positives (2+) were detected in 3 case of QDs-IHC with weak positives (1+) in IHC. The consistency test showed that the two methods showed good agreement (κ = 0.980, P = 0.014), but the sensitivity of QDs-IHC was slightly higher than that of conventional IHC. Our results show that QDs-IHC has strong sensitivity and high specificity. It is superior to conventional IHC in detecting HP infection in FFPE tissues of gastric biopsy, especially in tissues with low HP content.
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http://dx.doi.org/10.1007/s10735-020-09954-wDOI Listing
January 2021

A pacemaker-assisted microvascular decompression for a patient with left primary facial spasm and arrhythmia: a case report.

BMC Surg 2021 Jan 6;21(1):27. Epub 2021 Jan 6.

Neurosurgical Department, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China.

Background: Primary facial spasm accompanied by arrhythmia is a rare clinical phenomenon and has not been reported before. We describe this phenomenon and discuss its mechanism and treatment.

Case Presentation: We herein present a rare case of a patient with left primary facial spasm and a third-degree atrioventricular block (III degree AVB), who was implanted with a temporary cardiac pacemaker to receive microvascular decompression (MVD) because of refusal of a permanent cardiac pacemaker. The symptoms of facial spasm disappeared after MVD. The temporary cardiac pacemaker was removed on the second day after surgery. Her ECG still showed the third-degree atrioventricular block after a follow-up period of 5 months.

Conclusions: We are the first to report a patient with facial spasm and arrhythmia who was implanted with a temporary cardiac pacemaker to receive MVD. This case report demonstrated that the concomitant presence of a III degree AVB maybe not a contraindication for MVD, and the etiology of this facial spasm was the actual vascular compression of the facial nerve entry zone that was not related to the atrioventricular block.
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http://dx.doi.org/10.1186/s12893-020-01025-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789012PMC
January 2021

Application of Laser-Induced Breakdown Spectroscopy in Detection of Cadmium Content in Rice Stems.

Front Plant Sci 2020 18;11:599616. Epub 2020 Dec 18.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

The presence of cadmium in rice stems is a limiting factor that restricts its function as biomass. In order to prevent potential risks of heavy metals in rice straws, this study introduced a fast detection method of cadmium in rice stems based on laser induced breakdown spectroscopy (LIBS) and chemometrics. The wavelet transform (WT), area normalization and median absolute deviation (MAD) were used to preprocess raw spectra to improve spectral stability. Principal component analysis (PCA) was used for cluster analysis. The classification models were established to distinguish cadmium stress degree of stems, of which extreme learning machine (ELM) had the best effect, with 91.11% of calibration accuracy and 93.33% of prediction accuracy. In addition, multivariate models were established for quantitative detection of cadmium. It can be found that ELM model had the best prediction effects with prediction correlation coefficient of 0.995. The results show that LIBS provides an effective method for detection of cadmium in rice stems. The combination of LIBS technology and chemometrics can quickly detect the presence of cadmium in rice stems, and accurately realize qualitative and quantitative analysis of cadmium, which could be of great significance to promote the development of new energy industry.
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http://dx.doi.org/10.3389/fpls.2020.599616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775383PMC
December 2020

Wheat Kernel Variety Identification Based on a Large Near-Infrared Spectral Dataset and a Novel Deep Learning-Based Feature Selection Method.

Front Plant Sci 2020 10;11:575810. Epub 2020 Nov 10.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Near-infrared (NIR) hyperspectroscopy becomes an emerging nondestructive sensing technology for inspection of crop seeds. A large spectral dataset of more than 140,000 wheat kernels in 30 varieties was prepared for classification. Feature selection is a critical segment in large spectral data analysis. A novel convolutional neural network-based feature selector (CNN-FS) was proposed to screen out deeply target-related spectral channels. A convolutional neural network with attention (CNN-ATT) framework was designed for one-dimension data classification. Popular machine learning models including support vector machine (SVM) and partial least square discrimination analysis were used as the benchmark classifiers. Features selected by conventional feature selection algorithms were considered for comparison. Results showed that the designed CNN-ATT produced a higher performance than the compared classifier. The proposed CNN-FS found a subset of features, which made a better representation of raw dataset than conventional selectors did. The CNN-ATT achieved an accuracy of 93.01% using the full spectra and keep its high precision (90.20%) by training on the 60-channel features obtained via the CNN-FS method. The proposed methods have great potential for handling the analyzing tasks on other large spectral datasets. The proposed feature selection structure can be extended to design other new model-based selectors. The combination of NIR hyperspectroscopic technology and the proposed models has great potential for automatic nondestructive classification of single wheat kernels.
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http://dx.doi.org/10.3389/fpls.2020.575810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683420PMC
November 2020

A protocol of randomized controlled trial for Modified Xiaoyao Powder in the treatment of chronic obstructive pulmonary disease combined mild to moderate depression.

Medicine (Baltimore) 2020 Nov;99(47):e23220

Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China.

Introduction: Depression is an important complication of chronic obstructive pulmonary disease (COPD), occurring in more than one-third of individuals with COPD, and its severity is closely related to the severity and acute exacerbation of COPD, significantly contributing to the risk of death from COPD. Comorbid depression in COPD can be a burden on COPD-related diseases by reducing quality of life and compliance with treatment. Unfortunately, symptoms of COPD combined anxiety and depression are not properly diagnosed and treated in clinical practice, especially in the early stages of mood changes in patients with COPD, as the symptoms are mild and monotonous, and are overlooked.

Methods: In this prospective, randomized, placebo-controlled trial, we will assigned 280 eligible patients who had COPD combined depression to receive either Modified Xiaoyao Powder (MXP) or placebo. The primary end point is the change in the Hamilton Depression Scale (17 items) (HAMD-17) score from baseline on weeks 4, 12, and 24.

Discussion: Six months of MXP for COPD combined mild to moderate depression may alleviate the symptoms of depression, reduce the frequency of hospitalizations, the number of exacerbations, and improve the compliance of treatment.

Trial Registration: ChiCTR2000038741.
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http://dx.doi.org/10.1097/MD.0000000000023220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676530PMC
November 2020

Compositional differences of gut microbiome in matched hormone-sensitive and castration-resistant prostate cancer.

Transl Androl Urol 2020 Oct;9(5):1937-1944

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Background: It is known that gut microbiota can regulate cancer therapies. We hypothesized that gut microbiota may interact with androgen deprivation therapy (ADT) in the process of castration-resistant prostate cancer (CRPC). Here, the differences in gut microbiota between matched hormone-sensitive prostate cancer (HSPC) and CRPC were determined before and after ADT.

Methods: We profiled the fecal microbiota in matched HSPC and CRPC from 21 patients who received ADT at our urological center using 16S rRNA gene amplicon sequencing. Differences in microbiota were determined with α/β-diversity and LefSe analysis. Functional inference of microbiota was performed with PICRUSt.

Results: The results showed that the gut microbial community in CRPC was significantly altered with increased abundance of several bacterial flora including genus and . For functional analyses, bacterial gene pathways involved in terpenoids/polyketides metabolism and ether lipid metabolism were significantly activated in CRPC.

Conclusions: Measurable differences in the gut microbiota were identified between HSPC and CRPC. Functional validations are further needed to ascertain the underlying mechanism of these differential microbiota in the process of CRPC, and their potential as new targets to enhance ADT responses.
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http://dx.doi.org/10.21037/tau-20-566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658119PMC
October 2020

A randomized controlled trial for the effect of Modified Shenling Baizhu Powder on delaying the illness progress of COPD stable phase patients (GOLD 1-2 stages): A study protocol.

Medicine (Baltimore) 2020 Oct;99(43):e22700

Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Introduction: As one of the most prominent public health and medical problems, Chronic Obstructive Pulmonary Disease (COPD) has a serious impact on the quality of life of participants and may even be life-threatening. While modern medicine has worked well to alleviate the symptoms of COPD, the current situation with this chronic disease is not encouraging. Lung-spleen qi deficiency syndrome is one of the common forms of COPD and the traditional Chinese medicine formula Modified Shenling Baizhu Powder is very frequently used in the treatment of this syndrome. However, no direct evidence is available to support the efficacy and safety of Modified Shenling Baizhu Powder for COPD treatment.

Methods: The study is a prospective, randomized, placebo-controlled, double-blind trial in which 270 eligible participants will be randomly assigned to either the experimental or control group in a 1:1 ratio. Both groups will receive the standard Western medication. Meanwhile, participants in the experimental group will undergo Modified Shenling Baizhu Powder, while those in the control group will undergo a matched placebo. The course of treatment is 6 months with 12 months of follow-up. Primary outcome is the forced expiratory volume in 1 second (FEV1) after bronchodi-lator use. The secondary outcomes include the declines and the between-group difference in the change from baseline to 18 months in FEV1 before bronchodilator use; the forced vital capacity (FVC), FEV1/FVC, FEV1%pred after bronchodilator use, modified British medical research council, COPD Assessment Test, St George's Respiratory Questionnaire (SGRQ); frequency, interval, duration and severity of COPD exacerbations; time to first COPD exacerbation; administration of rescue medication and a cost-effectiveness analysis; Smoking status. A safety assessment will also be performed during the trial.

Discussion: The results of this trial will provide comprehensive evidence of the efficacy of Modified Shenling Baizhu Powder for early-stage COPD and the potential mechanism by which Modified Shenling Baizhu Powder acts, which may provide reference for the treatment plan of COPD participants.

Trial Registration: ChiCTR2000037873, Registered 2 September 2020.
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http://dx.doi.org/10.1097/MD.0000000000022700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581099PMC
October 2020

Tobacco-Related Exposure Upregulates Circ_0035266 to Exacerbate Inflammatory Responses in Human Bronchial Epithelial Cells.

Toxicol Sci 2021 Jan;179(1):70-83

State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, P.R. China.

One of the most carcinogenic chemicals found in cigarette tobacco smoke is 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), which has been confirmed to be associated with the etiology of diverse cancers. Lipopolysaccharide (LPS), another biologically active component of cigarette smoke, is a risk factor which enhances NNK-induced lung tumorigenesis due to chronic lung inflammation. Although inflammatory responses play critical roles in the initiation of many tumors, our knowledge about the mechanisms of NNK+LPS on inflammation is currently limited. Here, we investigated the inflammatory effects of NNK+LPS in human bronchial epithelial cells (BEAS-2B) and explored the underlying mechanisms mediated by circular RNAs (circRNAs). We identified a novel circRNA, circ_0035266, which was strongly upregulated in NNK+LPS-induced BEAS-2B cells and enhanced the inflammatory responses to NNK+LPS by regulating the secretion of pro-inflammatory cytokines interleukin (IL)-6 and IL-8. Specifically, circ_0035266 knockdown alleviated NNK+LPS-induced inflammatory responses, whereas overexpression of circ_0035266 had the opposite effect. Moreover, dual-luciferase reporter and fluorescence in situ hybridization (FISH) assays verified that circ_0035266 bound to miR-181d-5p directly in the cytoplasm. qRT-PCR, dual-luciferase reporter assays, and Western blot analyses showed that DDX3X (DDX3) was the downstream target of miR-181d-5p and that DDX3X expression levels were modulated by circ_0035266. These results suggested that circ_0035266 served as a competitive endogenous RNA for miR-181d-5p to regulate DDX3X expression, which is involved in the modulation of NNK+LPS-induced inflammatory responses in BEAS-2B cells.
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http://dx.doi.org/10.1093/toxsci/kfaa163DOI Listing
January 2021

Randomized clinical trial: efficacy and tolerability of two different split dose of low-volume polyethylene glycol electrolytes for bowel preparation before colonoscopy in hospitalized children.

Pediatr Res 2020 Oct 26. Epub 2020 Oct 26.

Department of Pediatric Gastroenterology Unit, The First Hospital of Jilin University, Changchun, Jilin, China.

Background: Eighty milliliter per kilogram of polyethylene glycol (PEG) for bowel preparation (BP) has been recommended, but the amount of liquid orally without nasogastric intubation is difficult to achieve. This study is to compare the efficacy and tolerability of two different low-volume PEG electrolyte solutions for BP in children.

Methods: The randomized, double-blind, controlled trial enrolled 150 children aged 6-18 years undergoing colonoscopy in our center. Patients were randomly assigned to receive 60 ml/kg (PEG-ELS 60) or 40 ml/kg (PEG-ELS 40) of PEG electrolytes (PEG-ELS) 4000. The Boston Bowel Preparation Scale was used for bowel cleansing evaluation. Primary end point was overall colon cleansing. Tolerability was also evaluated.

Results: PEG-ELS 40 and PEG-ELS 60 had similar efficacy in bowel cleansing for both whole colon and various colonic segments. The proportions of patients experiencing any adverse symptoms, or those who were willing to have BP repeated if necessary were similar in both groups. More patients considered the BP solution easy to take and be satisfied with the preparation in PEG-ELS 40 than PEG-ELS 60.

Conclusions: Low volume of PEG-ELS for BP has good efficacy in bowel cleansing. PEG-ELS with 40 ml/kg volume was not inferior to that of 60 ml/kg.

Impact: PEG-ELS 40 and PEG-ELS 60 had similar efficacy in bowel cleansing for whole and various colonic segments. The proportions of patients experiencing any adverse symptoms, or those who were willing to have BP repeated if necessary were similar in both groups. More patients considered BP solution easy to take and be satisfied with the preparation in PEG-ELS 40 than PEG-ELS 60. This study showed that low-volume PEG-ELS monotherapy was effective in bowel cleansing and explored a possibly feasible BP method for pediatrics in China that PEG-ELS 40 was comparable to PEG-ELS 60 regimen.
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http://dx.doi.org/10.1038/s41390-020-01216-5DOI Listing
October 2020

Icaritin reduces prostate cancer progression via inhibiting high-fat diet-induced serum adipokine in TRAMP mice model.

J Cancer 2020 21;11(22):6556-6564. Epub 2020 Sep 21.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Obesity resulting from high-fat diets has a close relationship with the morbidity and mortality associated with Prostate cancer (PCa) in males. The anti-cancer role of Icaritin (ICT, a traditional Chinese herbal medicine) has been reported in several types of cancer including PCa. Adipokines are novel adipocyte-specific secretory protein, which plays a key role in the development of various diseases including obesity, diabetes, atherosclerosis, and cancer. However, the function of ICT and the molecular mechanisms underlying its role in PCa regression through modulation of adipokines have not been studied. Here, we assessed the anti-cancer properties of ICT under the influence of human epidermal growth factor receptor type 2 (HER2) pathway modulating adipokines in obese PCa models. In this study, we used transgenic adenocarcinoma of mouse prostate (TRAMP), a well-established animal model for the study of PCa pathogenesis. All the animals were fed on a high-fat diet (HFD with 40% fat) and divided into two groups, one received ICT solution of 30 mg/kg body bwt (i.p) while the other group served as control without any ICT treatment. The mortality rate, tumor formation and fat ratio were assessed by histopathological and magnetic resonance analysis at different time points of 20, 24 and 28 weeks. The protein expression of HER2 and serum levels of adipokines were measured using western blotting, IHC and multiplex immunoassays. The PCa grade in 12 TRAMP mice were longitudinally evaluated to visualize PCa development and progression upon post-surgery using PET/CT scanning. We observed that ICT treatment significantly reduces the total mortality rate of TRAMP mice ( = 0.045) and the percentage of prostate intraepithelial neoplasia (PIN) or PCa ( = 0.029). Interestingly, significantly decreased levels of leptin ( = 0.006 @20 wk) and the elevated levels of adiponectin ( = 0.030 @20 wk) were observed in different subgroups upon ICT treatment in a time-dependent manner. In addition, a decrease level of HER2 ( = 0.032 @28 wk) and an elevated level of PEA3 ( = 0.014 @28 wk) were also detected in ICT treated group. The PET/CT-based imaging showed that ICT vs non-ICT treated mice had different standard uptake value and metastasis. Our results showed potent anti-cancer properties of ICT through the modulation of adipokine secretion may alter the expression and activation of HER2 pathway as an alternative mechanism to prevent PCa progression. Altogether, our findings indicate that ICT could be a promising cancer preventive agent with the potential to target and eradicate tumor cells in obese PCa patients.
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http://dx.doi.org/10.7150/jca.48413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545683PMC
September 2020

Sewage as a Possible Transmission Vehicle During a Coronavirus Disease 2019 Outbreak in a Densely populated Community: Guangzhou, China, April 2020.

Clin Infect Dis 2020 Oct 12. Epub 2020 Oct 12.

Guangzhou Center for Disease Control and Prevention, Guangzhou, China.

Background: SARS-CoV-2 has been identified in the fecal matter of COVID-19 patients. However, sewage transmission has never been shown. In April 2020, a COVID-19 outbreak occurred in a densely populated community in Guangzhou, China. We investigated this outbreak to identify the mode of transmission.

Method: A home quarantined order was issued in the community. We collected throat swab samples from the residents and environmental samples from the surfaces inside and around the houses, and conducted RT-PCR testing and genome sequencing. We defined a case as a resident in this community with a positive RT-PCR test, with or without symptoms. We conducted a retrospective cohort study of all residents living in the same buildings as the cases to identify exposure risk factors.

Result: We found eight cases (four couples) in this community of 2888 residents (attack rate=2.8/1000), with onset during April 5-21, 2020. During their incubation periods, Cases 1-2 frequented market T with an ongoing outbreak. Cases 3-8 never visited market T during incubation period, lived in separate buildings from, and never interacted with, Cases 1-2. Retrospective cohort study showed that working as cleaners or waste picker (RR=13, 95% CIexact: 2.3-180), not changing to clean shoes after returning home (RR=7.4, 95% CIexact: 1.8-34), collating and cleaning dirty shoes after returning home (RR=6.3, 95% CIexact: 1.4-30) were significant exposure risk factors. Of 63 samples collected from street-sewage puddles and sewage-pipe surfaces, 19% tested positive for SARS-CoV-2. Of 50 environmental samples taken from cases' apartments, 24% tested positive. Viral genome sequencing showed that the viruses identified from the squat toilet and shoe-bottom dirt inside the apartment of Cases 1-2 were homologous with those from Cases 3-8 and those identified from sewage samples. The sewage pipe leading from the apartment of Cases 1-2 to the drainage had a large hole above ground. Rainfalls after the onset of Cases 1-2 flooded the streets.

Conclusion: Our investigation has for the first time pointed to the possibility that SARS-CoV-2 might spread by sewage. This finding highlighted the importance of sewage management, especially in densely-populated places with poor hygiene and sanitation measures, such as urban slums and other low-income communities in developing countries.
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http://dx.doi.org/10.1093/cid/ciaa1494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665342PMC
October 2020

Comparative transcriptomic analysis of the tea plant (Camellia sinensis) reveals key genes involved in pistil deletion.

Hereditas 2020 Sep 8;157(1):39. Epub 2020 Sep 8.

Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai, 666201, China.

Background: The growth process of the tea plant (Camellia sinensis) includes vegetative growth and reproductive growth. The reproductive growth period is relatively long (approximately 1.5 years), during which a large number of nutrients are consumed, resulting in reduced tea yield and quality, accelerated aging, and shortened economic life of the tea plant. The formation of unisexual and sterile flowers can weaken the reproductive growth process of the tea plant. To further clarify the molecular mechanisms of pistil deletion in the tea plant, we investigated the transcriptome profiles in the pistil-deficient tea plant (CRQS), wild tea plant (WT), and cultivated tea plant (CT) by using RNA-Seq.

Results: A total of 3683 differentially expressed genes were observed between CRQS and WT flower buds, with 2064 upregulated and 1619 downregulated in the CRQS flower buds. These genes were mainly involved in the regulation of molecular function and biological processes. Ethylene synthesis-related ACC synthase genes were significantly upregulated and ACC oxidase genes were significantly downregulated. Further analysis revealed that one of the WIP transcription factors involved in ethylene synthesis was significantly upregulated. Moreover, AP1 and STK, genes related to flower development, were significantly upregulated and downregulated, respectively.

Conclusions: The transcriptome analysis indicated that the formation of flower buds with pistil deletion is a complex biological process. Our study identified ethylene synthesis, transcription factor WIP, and A and D-class genes, which warrant further investigation to understand the cause of pistil deletion in flower bud formation.
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http://dx.doi.org/10.1186/s41065-020-00153-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487804PMC
September 2020

Cognitive impairment in a classical rat model of chronic migraine may be due to alterations in hippocampal synaptic plasticity and N-methyl-D-aspartate receptor subunits.

Mol Pain 2020 Jan-Dec;16:1744806920959582

Department of Neurology, the First Medical Centre, Chinese PLA General Hospital, PR China.

Although migraine is a major global public health problem, its impact on cognitive abilities remains controversial. Thus, the present study investigated the effects of repeated administration of inflammatory soup to the dura of rats, over three weeks, on spatial cognition, hippocampal synaptic plasticity, and the expression of N-methyl-D-aspartate receptor subunits. Additionally, low doses of amitriptyline (5 mg/kg) were applied to assess its therapeutic effects. The inflammatory soup group exhibited significant reductions in the cutaneous stimulation threshold, presence of mild cognitive impairment, and decreased long-term potentiation in right hippocampus. However, amitriptyline improved pain behaviors, enhanced cognitive function, and increased synaptic plasticity in the inflammatory soup rats. On the other hand, the administration of amitriptyline to normal rats negatively influenced synaptic plasticity and reduced the expression of N-methyl-D-aspartate receptor subunits. The present results indicate that inflammatory soup-induced dural nociception led to impairments in spatial cognition that could be attributed to reductions in hippocampal long-term potentiation and the decreased expression of N-methyl-D-aspartate receptor subunits.
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http://dx.doi.org/10.1177/1744806920959582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517984PMC
September 2020

Carbon dots-based dual-emission ratiometric fluorescence sensor for dopamine detection.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Dec 10;243:118804. Epub 2020 Aug 10.

Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China; Collaborative Innovation Center for Brain Science, Chongqing University, Chongqing 400044, China; Centre for NanoHealth, College of Engineering, Swansea University, Singleton Park, Swansea SA2 8PP, UK. Electronic address:

The detection of Dopamine (DA) is significant for disease surveillance and prevention. However, the development of the precise and simple detection techniques is still at a preliminary stage due to their high tester requirements, time-consuming process, and low accuracy. In this work, we present a novel dual-emission ratiometric fluorescence sensing system based on a hybrid of carbon dots (CDs) and 7-amino-4-methylcoumarin (AMC) to quickly monitor the DA concentration. Linked via amide bonds, the CDs and AMC offered dual-emissions with peaks located at 455 and 505 nm, respectively, under a single excitation wavelength of 300 nm. Attributed to the fluorescence of the CDs and AMC in the nanohybrid system can be quenched by DA, the concentration of DA could be quantitatively detected by monitoring the ratiometric ratio change in fluorescent intensity. More importantly, the CDs-AMC-based dual-emission ratiometric fluorescence sensing system demonstrated a remarkable linear relationship in the range of 0-33.6 μM to detection of DA, and a low detection limit of 5.67 nM. Additionally, this sensor successfully applied to the detection of DA in real samples. Therefore, the ratiometric fluorescence sensing system may become promising to find potential applications in biomedical dopamine detection.
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http://dx.doi.org/10.1016/j.saa.2020.118804DOI Listing
December 2020

A parametric and compact nanosecond pulsed power generator for liquid phase discharge.

Rev Sci Instrum 2020 Jul;91(7):074709

College of Electrical Engineering and Control Science, Nanjing Tech University, Nanjing 211816, China.

Nanosecond pulsed liquid discharge has attracted significant attention in various applications, in which adjustable parameters and compact volume of nanosecond pulsed power are essential for the convenience of researchers. In this paper, a compact volume (0.6 × 0.8 × 0.4 m) nanosecond pulsed power supply is built for the liquid discharge with the capacity charging power supply technology. Moreover, a high-frequency induction feed control system is adopted to achieve synchronizations between insulated gate bipolar transistors to realize the adjustable pulse width and frequency. A non-inductive resistor is used to test the properties of the nanosecond pulse power supply, and results show that the rising time of nanosecond pulse power is 100 ns with the pulse width in the range of 4 µs to ∼100 µs, and the output pulse voltage and repetition frequency are 0 kV-20 kV and 1 Hz-300 Hz, respectively. Moreover, the needle-to-needle electrode discharge in the liquid phase is successfully excited by this power supply with different working conditions.
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http://dx.doi.org/10.1063/1.5142267DOI Listing
July 2020

Vision-Based Moving Obstacle Detection and Tracking in Paddy Field Using Improved Yolov3 and Deep SORT.

Sensors (Basel) 2020 Jul 22;20(15). Epub 2020 Jul 22.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Using intelligent agricultural machines in paddy fields has received great attention. An obstacle avoidance system is required with the development of agricultural machines. In order to make the machines more intelligent, detecting and tracking obstacles, especially the moving obstacles in paddy fields, is the basis of obstacle avoidance. To achieve this goal, a red, green and blue (RGB) camera and a computer were used to build a machine vision system, mounted on a transplanter. A method that combined the improved You Only Look Once version 3 (Yolov3) and deep Simple Online and Realtime Tracking (deep SORT) was used to detect and track typical moving obstacles, and figure out the center point positions of the obstacles in paddy fields. The improved Yolov3 has 23 residual blocks and upsamples only once, and has new loss calculation functions. Results showed that the improved Yolov3 obtained mean intersection over union (mIoU) score of 0.779 and was 27.3% faster in processing speed than standard Yolov3 on a self-created test dataset of moving obstacles (human and water buffalo) in paddy fields. An acceptable performance for detecting and tracking could be obtained in a real paddy field test with an average processing speed of 5-7 frames per second (FPS), which satisfies actual work demands. In future research, the proposed system could support the intelligent agriculture machines more flexible in autonomous navigation.
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http://dx.doi.org/10.3390/s20154082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436064PMC
July 2020

Circular RNA circBbs9 promotes PM-induced lung inflammation in mice via NLRP3 inflammasome activation.

Environ Int 2020 10 21;143:105976. Epub 2020 Jul 21.

State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China; Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou 511436, China. Electronic address:

Fine particulate matter (PM) is one of the most important components of environmental pollutants, and is associated with pulmonary injury. However, the biological mechanisms of pulmonary damage caused by PM are poorly defined, especially the molecular pathways related to inflammation. Following system exposure to PM for 3 months in normal mice and in chronic obstructive pulmonary disease (COPD) model mice, it was found that PM exposure increased the expression of IL-1β and IL-18 in lung tissues via NLRP3 activation, and these effects were more intense in COPD model mice. Circular RNA (circRNA) sequencing showed that the expression profiles of circRNAs were changed after PM exposure, and the positive roles of circBbs9 in inflammation induced by PM were verified. The circBbs9 knockdown alleviated PM-induced inflammation via NLRP3 inflammasome inactivation, as well as IL-1β and IL-18 inhibition in RAW264.7 cells, while overexpression of circBbs9 had the opposite effect. Bioinformatics and luciferase reporter assays showed that circBbs9 bound to microRNA-30e-5p (miR-30e-5p) and co-regulated the expression of Adar, a downstream target gene of miR-30e-5p. Taken together, these results revealed that PM induced pulmonary inflammation through NLRP3 inflammasome activation regulated by the circBbs9-miR-30e-5p-Adar pathway. Our findings provide a new target, circBbs9, for the assessment of lung inflammation and COPD exacerbation induced by PM exposure.
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http://dx.doi.org/10.1016/j.envint.2020.105976DOI Listing
October 2020

Crystallization Morphology Regulation on Enhancing Heat Resistance of Polylactic Acid.

Polymers (Basel) 2020 Jul 15;12(7). Epub 2020 Jul 15.

National Engineering Research Center for Compounding and Modification of Polymeric Materials, Guiyang 550014, China.

To expand the use of polylactic acid (PLA) in high-temperature environments, crystallization morphology regulation was studied to enhance the heat resistance of PLA. PLA crystallinity was controlled using heat treatment and nucleating agent (zinc phenylphosphonate, brand TMC). The heat deflection temperatures of PLAs with same crystallinities considerably varied using different treatments. The crystallization morphology of PLA (4032D) and PLA/TMC composites was studied using X-ray diffraction (XRD) and polarized optical microscopy. XRD test results show that TMC can improve the crystallization rate and heat treatment can enhance the crystallinity and thickness of PLA, suggesting that the crystallization morphology improved after heat treatment. Nucleating agents can increase the crystallinity of PLA but cannot improve its crystallization morphology. The findings indicate that at the same crystallinity, PLAs exhibit improved crystallization morphology and high heat resistance; these results can provide guidance for improving the heat resistance of PLAs and facilitate the design of new nucleating agents.
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http://dx.doi.org/10.3390/polym12071563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407181PMC
July 2020

Ductal Carcinoma In Situ of the Breast: Perspectives on Tumor Subtype and Treatment.

Biomed Res Int 2020 27;2020:7251431. Epub 2020 May 27.

Institute of Pathology, China Three Gorges University, Yichang 443000, China.

Objective: To evaluate ductal carcinoma in situ (DCIS) characteristics and the effect of different treatment strategies. . Using data with known hormone receptor (HoR) and human epidermal growth factor receptor 2 (HER2) status obtained by the Surveillance, Epidemiology, and End Results (SEER) program from 2010-2014, the study was conducted to investigate tumor subtype-specific differences in various characteristics, overall survival (OS), and breast cancer-specific mortality (BCSM).

Results: A total of 3415 patients with DCIS were eligible. Compared with HoR+/HER- subgroup, patients with triple-negative (TN) and HoR-/HER+ were commonly higher in grade, larger in size, and tended to receive mastectomy ( < 0.05). The multivariate analysis revealed that patients with TN were more likely to have a poorer OS and show a higher breast cancer-specific mortality compared with the HoR+/HER- subgroup ( < 0.05). Multivariate analysis on the history of local treatment and surgery showed patients receiving breast-conserving surgery (BCS) plus radiotherapy (R) and BCS plus axillary lymph node dissection was likely to improve OS without affecting breast cancer-specific mortality ( < 0.05).

Conclusion: The results demonstrate that DCIS associated with TN subtype portends poor prognosis. Meanwhile, BCS plus R was a preferable option and resulted in survival rates better than those achieved with mastectomy, and SLNB should be considered as an appropriate assessment of axillary staging in patients with DCIS.
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http://dx.doi.org/10.1155/2020/7251431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275239PMC
March 2021

Combination of Anti-Programmed Death 1 Therapy and Apatinib for a Patient with Hepatocellular Carcinoma and Brain Metastasis: Case Report and Literature Review.

World Neurosurg 2020 11 10;143:114-117. Epub 2020 Jun 10.

Neurosurgical Department, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. Electronic address:

Background: Brain metastasis is rare in patients with hepatocellular carcinoma (HCC). The combination of an anti-programmed death 1 inhibitor and an anti-vascular endothelial growth factor drug provides therapeutic opportunities for refractory patients. So far, there are no data on the efficacy of these combined therapies for patients with HCC brain metastasis.

Case Description: HCC brain metastasis was diagnosed in a 31-year-old man. First, he underwent left liver resection and cholecystectomy and recovered well postoperatively. The postoperative pathologic findings were consistent with HCC. Approximately 2 years later, he presented with persistent headache and underwent magnetic resonance imaging, which revealed a lesion in the left parietal lobe. Anti-programmed death 1 and anti-vascular endothelial growth factor drugs were administered in combination, but the lesion did not shrink after the combined treatment on repeat magnetic resonance imaging. Therefore, surgical removal of the brain lesion was performed 2 months later. The postoperative pathologic results showed coagulative necrosis.

Conclusions: This case report highlights the efficacy of toripalimab and apatinib in the management of brain metastasis from HCC.
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http://dx.doi.org/10.1016/j.wneu.2020.06.015DOI Listing
November 2020

Changes in rhizobacterial community mediating atrazine dissipation by arbuscular mycorrhiza.

Chemosphere 2020 Oct 12;256:127046. Epub 2020 May 12.

Northeast Forestry University, Harbin, 150040, China. Electronic address:

Although it was well known that arbuscular mycorrhizal fungus (AMF) inoculation significantly increased atrazine dissipation in the soil, the effect of AMF on bacterial community, especially potential atrazine-degrading bacteria mediating atrazine dissipation has been overlooked. In the present study, there were four different treatments: Funnelliformis mosseae inoculation with or without atrazine; and non-AMF inoculation with or without atrazine. F. mosseae significantly increased atrazine dissipation rate from 28.7% to 53.3%. Then 16S rRNA gene sequencing results indicated that bacteria community differed significantly by F. mosseae inoculation and atrazine addition. The Shannon index decreased significantly with AMF and atrazine at phylum and family level, and significant inhibition of atrazine on evenness was also observed. LEFSe analysis revealed that Terrimonas and Arthrobacter were significantly associated with F. mosseae, as well as unidentified_Nitrospiraceae associated with atrazine addition. There are several bacterial taxa associated with both F. mosseae inoculation and atrazine addition. Totally, twelve atrazine-degrading bacterial genera (>0.10%) were identified. When atrazine was added, the abundance of Arthrobacter, Burkholderia, Mycobacterium and Streptomyces increased in F. mosseae inoculation treatment, but Nocardioides, Pseudomonas, Bradyrhizobium, Rhizobium, Rhodobacter, Methylobacterium, Bosea and Shinella decreased. In the presence of atrazine, activities of dehydrogenase, urease, acid and alkaline phosphatase in F. mosseae inoculation treatment were significantly higher than those in non-inoculation. However, there was no significant relationship between bacterial community and any soil enzyme activity in four treatments. Our findings reveal the potential relationship between soil bacterial community and AMF inoculation during atrazine dissipation.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127046DOI Listing
October 2020

Predicting per-lesion local recurrence in locally advanced non-small cell lung cancer following definitive radiation therapy using pre- and mid-treatment metabolic tumor volume.

Radiat Oncol 2020 May 19;15(1):114. Epub 2020 May 19.

Department of Radiation Oncology, Stanford University School of Medicine and Stanford Cancer Institute, 875 Blake Wilbur Dr MC 5847, Stanford, CA, 94305, USA.

Background: We evaluated whether pre- and mid-treatment metabolic tumor volume (MTV) predicts per lesion local recurrence (LR) in patients treated with definitive radiation therapy (RT, dose≥60 Gy) for locally advanced non-small cell lung cancer (NSCLC).

Methods: We retrospectively reviewed records of patients with stage III NSCLC treated from 2006 to 2018 with pre- and mid-RT PET-CT. We measured the MTV of treated lesions on the pre-RT (MTV) and mid-RT (MTV) PET-CT. LR was defined per lesion as recurrence within the planning target volume. Receiver operating characteristic (ROC) curves, cumulative incidence rates, and uni- and multivariable (MVA) competing risk regressions were used to evaluate the association between MTV and LR.

Results: We identified 111 patients with 387 lesions (112 lung tumors and 275 lymph nodes). Median age was 68 years, 69.4% were male, 46.8% had adenocarcinoma, 39.6% had squamous cell carcinoma, and 95.5% received concurrent chemotherapy. Median follow-up was 38.7 months. 3-year overall survival was 42.3%. 3-year cumulative incidence of LR was 26.8% per patient and 11.9% per lesion. Both MTV and MTV were predictive of LR by ROC (AUC = 0.71 and 0.76, respectively) and were significantly associated with LR on MVA (P = 0.004 and P = 7.1e-5, respectively). Among lesions at lower risk of LR based on MTV, higher MTV was associated with LR (P = 0.001).

Conclusion: Per-lesion, larger MTV and MTV predicted for increased risk of LR. MTV was more highly predictive of LR than MTV and if validated may allow for further discrimination of high-risk lesions at mid-RT informing dose painting strategies.
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http://dx.doi.org/10.1186/s13014-020-01546-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238662PMC
May 2020