Publications by authors named "Yufang Gu"

8 Publications

  • Page 1 of 1

Preparation of a novel monoclonal antibody against Avian leukosis virus subgroup J Gp85 protein and identification of its epitope.

Poult Sci 2021 Jul 12;100(7):101108. Epub 2021 Mar 12.

School of Animal Science, Yangtze University, Jingzhou 434025, China. Electronic address:

Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused huge economic losses in the poultry industry due to its great pathogenicity and transmission ability. However, the continuous emergence of new strains would bring challenges to diagnosis and control of ALV-J. .This study focuses on preparing the monoclonal antibody (MAb) against ALV-J Gp85 and identifying its epitope. The truncated ALV-J gp85 gene fragment was amplified and then cloned into expression vectors. Purified GST-Gp85 was used to immune mice and His-Gp85 was used to screen MAb. Finally, a hybridoma cell line named J16 that produced specific MAb against the ALV-J. Immunofluorescence assay showed that MAb J16 specifically recognized ALV-J rather than ALV-A or ALV-K infected DF-1 cells. To identify the epitope recognized by MAb J16, fourteen partially overlapping ALV-J Gp85 fragments were prepared and tested by Western blot. The results indicated that peptide 150-LIRPYVNQ-157 was the minimal epitope of ALV-J Gp85 recognized by MAb J16. Alignment analysis of Gp85 from different ALV subgroups showed that the epitope keep high conservation among 36 ALV-J strains, but significant different from that of ALV subgroup A, B, C, D, E and K. Overall, we prepared a MAb specific against ALV-J and identified peptide 150-LIRPYVNQ-157 as a novel specific epitope of ALV-J Gp85, which may assist in laying the foundation for specific ALV-J detection methods.
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http://dx.doi.org/10.1016/j.psj.2021.101108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192869PMC
July 2021

Identification of a novel epitope specific for Gp85 protein of avian leukosis virus subgroup K.

Vet Immunol Immunopathol 2020 Dec 20;230:110143. Epub 2020 Oct 20.

College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China. Electronic address:

During the past two decades, avian leukosis virus (ALV) caused tremendous economic losses to poultry industry in China. ALV-K as a newly found subgroup in recent years, which made the control and eradication of ALV more difficult as they were originated from the recombination of different subgroups. To date, specific rapid detection methods refer to ALV-K are still missing. Gp85 is the main structural protein of the virus, which mediates the invasion of host cells by the virus and determinates the classification of subgroups. In this study, we prepared a monoclonal antibody (Mab) named Km3 against Gp85 of ALV-K. Immunofluorescence assay showed that Km3 specifically recognized the strains of ALV-K rather than the strains of ALV-A or ALV-J. To explain the subgroups specificity of Km3, the epitope cognized by the Mab was identified by Western blotting using 15 overlapping fragments spanning the Gp85. Finally, the peptide AFGPRSIDTLSDWSRPQ was identified as the minimal linear epitope recognized by Km3. Alignment of Gp85 from different subgroups showed that the epitope was highly conserved among ALV-K strains, which was quite different from that of the strains from ALV -A, -B and -J. In conclusion, the Mab Km3 may serve as a useful reagent for ALV-K detection and diagnosis in the future.
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http://dx.doi.org/10.1016/j.vetimm.2020.110143DOI Listing
December 2020

Identification and characterization of a novel natural recombinant avian leucosis virus from Chinese indigenous chicken flock.

Virus Genes 2019 Oct 8;55(5):726-733. Epub 2019 Aug 8.

School of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou, 434025, China.

Avian leukosis virus (ALV) caused tremendous economic losses to poultry industry all over the world, especially in China. One natural recombinant ALV strain, designated as HB2015032, was isolated from indigenous chickens with neoplastic diseases in Hubei, China. The complete proviral genome of HB2015032 is 7703 bp in length. Sequence analysis showed that the Env of HB2015032 exhibited 99.3% similarity with that of a ALV subgroup K (ALV-K) isolate JS11C1 at amino acid level. Phylogenetic analysis revealed that both gp85 and gp37 of HB2015032 were clustered in the same branch with JS11C1 and other ALV-K strains isolated from Chinese indigenous chickens in recent years. However, the pol gene, the 3' untranslated region (3' UTR), and the 3' long terminal repeat (3' LTR) of HB2015032 were more closely related to ALV-J prototype HPRS-103, and clustered in the same branch with ALV-J strains. Furthermore, the pol gene of HB2015032 contained a premature stop codon that resulted in a truncated Pol protein with 22 amino acid residues missing, which was a unique feature of the pol gene of ALV-J. 3'UTR of HB2015032 containing entire DR1, E element and U3. E element of HB2015032 contained one base deletion, which resulted in a c-Ets-1 binding site. In addition, U3 region of HB2015032 contains most of the transcription regulatory elements of ALV-J, including two CAAT boxes, Y boxes, CArG boxes, PRE boxes, NFAP-1 boxes, and one TATA box. These results suggest that isolate HB2015032 was a novel recombinant ALV-K containing the ALV-K env gene and the ALV-J backbone and exhibiting high pathogenicity.
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http://dx.doi.org/10.1007/s11262-019-01695-7DOI Listing
October 2019

Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma.

Cell Mol Biol Lett 2018 27;23:17. Epub 2018 Apr 27.

Department of Medical Care, Zibo Central Hospital, 54 Gongqingtuan Rd, Zibo, Shandong 255036 People's Republic of China.

Background: Epstein-Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC.

Methods: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC.

Results: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo.

Conclusions: These data indicate the pro-oncogenic potential of SNHG8 in EBV-associated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer.
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http://dx.doi.org/10.1186/s11658-018-0070-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924468PMC
October 2018

Outbreak of Listeria Monocytogenes in Pheasants.

Poult Sci 2015 Dec 16;94(12):2905-8. Epub 2015 Oct 16.

College of Animal Science, Yangtze University, Jingzhou 434025, P.R. China

Listeria monocytogenes is capable of infecting almost all animals. However, outbreaks of listeriosis are infrequent in birds. This report describes an outbreak of listeriosis in a small pheasant (Phasianus colchicus) breeder farm with more than 2,000 pheasants from Hubei province of the People's Republic of China. The affected flock consisted of adult and young birds. Approximately 300 young birds and a few adult birds were found dead within a few days of the onset of clinical signs. Twenty-five dead birds were collected for further examination. Histopathological lesions in the visceral organs were characterized by monocyte infiltration and proliferation. Localized encephalitis and meningitis were detected in the brains of dead birds. Gram-positive organisms were observed in heart blood smear, liver, and brain impression smears. The organisms were isolated from fresh liver and were identified as L. monocytogenes serotype 4b based on multiplex polymerase chain reaction (PCR) and hlyA gene sequence analysis. This is the first report describing outbreak of listeriosis in pheasant flock.
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http://dx.doi.org/10.3382/ps/pev264DOI Listing
December 2015

Rhubarb to Facilitate Placement of Nasojejunal Feeding Tubes in Patients in the Intensive Care Unit.

Nutr Clin Pract 2016 Feb 12;31(1):105-10. Epub 2015 Oct 12.

Department of Intensive Care Unit, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China

Background: Prokinetic agents are sometimes needed to aid in the placement of nasojejunal feeding tubes in patients at risk of malnutrition. The objective of the present study was to evaluate the feasibility of rhubarb as a new prokinetic agent to replace metoclopramide and erythromycin in the placement of nasojejunal feeding tubes.

Materials And Methods: Ninety-four patients who required jejunal feeding tube insertion were included. They were divided into rhubarb (n = 34), metoclopramide (n = 31), and erythromycin groups (n = 29), depending on the use of rhubarb, metoclopramide, and erythromycin as the prokinetic agent. The jejunal feeding tube insertions were performed at the bedside. An abdominal x-ray was taken as the gold standard to determine the position of the tube. Cases in which insertion failed in either group were subjected to a second insertion attempt using rhubarb as the prokinetic agent.

Results: The success rates in the rhubarb, metoclopramide, and erythromycin groups were 91.2%, 87.1%, and 89.7%, respectively. The difference in the success rates was not statistically significant (P = .916). The insertion times in the rhubarb, metoclopramide, and erythromycin groups were 16.0 ± 1.9 minutes, 18.0 ± 1.9 minutes, and 18.8 ± 2.2 minutes, respectively. The insertion time in the rhubarb group was significantly shorter than those in metoclopramide and erythromycin groups (P < .001). No side effects were noted in the rhubarb group.

Conclusions: Rhubarb could serve as an effective prokinetic agent to promote the insertion of nasojejunal feeding tubes.
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http://dx.doi.org/10.1177/0884533615608363DOI Listing
February 2016

The combination of inferior pedicle method and dermal suspension sling technique: one new efficient method for breast reduction.

Int J Clin Exp Med 2015 15;8(4):6613-20. Epub 2015 Apr 15.

Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University Hefei 230022, China.

Objective: To explore a method of breast reduction which ensures well nipple and areola lactation function, adequate blood supply and good medial fullness and projection. At the same time, this study could evaluate the advantages of the combination of inferior pedicle method and dermal suspension sling technique for breast reduction.

Methods: From 2011.11 to 2013.8, 13 women have undergone breast reduction using utilizing inferior pedicle combined with the dermal suspension sling technique. The inferior pedicle was designed with medial and lateral triangular flaps in the areas where normally be excised. These triangular flaps were deepithelialized and defatted. The flaps were attached to the chest wall above the inferior pedicle to create a dermal "cage".

Results: After operation, Sensation of nipple and areola complex, breast projection and shape were sustained during follow-up, of which the median interval was 12 months. No patient had poor projection and bottoming out.

Conclusion: Dermal suspension and horizontal dermal placation provides a structural foundation to the inferior pedicle. It is an effective method of treatment for breast reduction, in that the sensation and lactation function of nipple and areola complex get further guaranteed, have nice breast projection and shape, and can be applied to all cases of breast reduction.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4484012PMC
July 2015

Eating, activity, and supportive environment (EASE) for nursing students.

Nurs Forum 2014 Jan-Mar;49(1):49-58. Epub 2013 Jul 8.

College of Nursing, Texas Woman's University, Denton, TX.

Purpose: The feasibility study was designed as a potential model for prevention of obesity at a large college of nursing in the Southwest.

Conclusions: Pretest median body mass index was 30.4 in the intervention group and 30.5 in the control group, indicating obesity in the student sample. There were no significant changes in posttest measures in the small sample. The thigh circumference change in the experimental group trended toward change with significance of .06.

Implication: The American Nursing Association launched a Healthy Nurse program in 2012, as healthy nurses are role models for a healthy nation. Updating nursing curriculum and active participation of students in weight management programs will promote their own health and positive role modeling.
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http://dx.doi.org/10.1111/nuf.12047DOI Listing
October 2015
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