Publications by authors named "Yue Zhao"

2,128 Publications

  • Page 1 of 1

Reversing multi-drug resistance by polymeric metformin to enhance antitumor efficacy of chemotherapy.

Int J Pharm 2022 Jun 21:121931. Epub 2022 Jun 21.

School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China; Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China. Electronic address:

Multi-drug resistance (MDR) in breast cancer poses a great threat to chemotherapy. The expression and function of the ATP binding cassette (ABC) transporter are the major cause of MDR. Herein, a linear polyethylene glycol (PEI) conjugated with dicyandiamide, which called polymeric metformin (PolyMet), was successfully synthesized as a simple and biocompatible polymer of metformin. PolyMet showed the potential to reverse MDR by inhibiting the efflux of the substrate of ATP-binding cassette (ABC) transporter from DOX resistant MCF-7 cells (MCF-7/DOX). To test its MDR reversing effect, PolyMet was combined with DOX to treat mice carrying MCF-7/DOX xenografts. In order to decrease the toxicities of DOX and delivery PolyMet and DOX to tumor at the same time, PolyMet was complexed with poly-γ-glutamic acid-doxorubicin (PGA-DOX) electrostatically at the optimal ratio of 2 : 3, which were further coated with lipid membrane to form lipid/PolyMet-(PGA-DOX) nanoparticles (LPPD). The particle size of LPPD was 165.8 nm, and the zeta potential was +36.5 mV. LPPD exhibited favorable cytotoxicity and cellular uptake in MCF-7/DOX. Meanwhile, the bioluminescence imaging and immunohistochemical analysis indicated that LPPD effectively conquered DOX-associated MDR by blocking ABC transporters (ABCB1 and ABCC1) via PolyMet. Remarkably, LPPD significantly inhibited the tumor growth and lowered the systemic toxicity in a murine MCF-7/DOX tumor model. This is the first time to reveal that PolyMet can enhance the anti-tumor efficacy of DOX by dampening ABC transporters and activating the AMPK/mTOR pathway, which is a promising strategy for drug-resistant breast cancer therapy.
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http://dx.doi.org/10.1016/j.ijpharm.2022.121931DOI Listing
June 2022

Seaweed-Derived Polysaccharides Attenuate Heat Stress-Induced Splenic Oxidative Stress and Inflammatory Response via Regulating Nrf2 and NF-κB Signaling Pathways.

Mar Drugs 2022 May 27;20(6). Epub 2022 May 27.

Department of Animal Science, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

With global warming, heat stress (HS) has become a worldwide concern in both humans and animals. The ameliorative effect of seaweed () derived polysaccharides (SDP) on HS-induced oxidative stress and the inflammatory response of an immune organ (spleen) was evaluated using an animal model (). In total, 144 animals were used in this 4-week trial and randomly assigned to the following three groups: thermoneutral zone, HS, and HS group supplemented with 1000 mg/kg SDP. Dietary SDP improved the antioxidant capacity and reduced the malondialdehyde (MDA) of the spleen when exposed to HS, regulated via enhancing nuclear factor erythroid 2-related factor-2 (Nrf2) signaling. Furthermore, the inclusion of SDP reduced the levels of pro-inflammatory cytokines and alleviated HS-induced splenic inflammatory response by suppressing the nuclear factor-kappa B (NF-κB) p65 signaling. These findings suggest that the SDP from can be used as a functional food and/or feed supplement to attenuate HS-induced oxidative stress and inflammatory responses of the immune organs. Moreover, the results could contribute to the development of high-value marine products from seaweed for potential use in humans and animals, owing to their antioxidant and anti-inflammatory effects.
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http://dx.doi.org/10.3390/md20060358DOI Listing
May 2022

Low Band Gap Perovskite Concentrator Solar Cells: Physics, Device Simulation, and Experiment.

ACS Appl Mater Interfaces 2022 Jun 22. Epub 2022 Jun 22.

School of Optoelectronic Science and Engineering and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215006, China.

Perovskite solar cells (PSCs) own rapidly increasing power conversion efficiencies (PCEs), but their concentrated counterparts (i.e., PCSCs) show a much lower performance. A deeper understanding of PCSCs relies on a thorough study of the intensive energy losses of the device along with increasing the illumination intensity. Taking the low band gap Sn-Pb PCSC as an example, we realize a device-level optoelectronic simulation to thoroughly disclose the internal photovoltaic physics and mechanisms by addressing the fundamental electromagnetic and carrier-transport processes within PCSCs under various concentration conditions. We find that the primary factor limiting the performance improvement of PCSCs is the significantly increased bulk recombination under the increased light concentration, which is attributed mostly to the inferior transport/collection ability of holes determined by the hole transport layer (HTL). We perform further electrical manipulation on the perovskite layer and the HTL so that the carrier-transport capability is significantly improved. Under the optoelectronic design, we fabricate low band gap PCSCs, which exhibit particularly high PCEs of up to 22.36% at 4.17 sun.
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http://dx.doi.org/10.1021/acsami.2c06393DOI Listing
June 2022

Electrically driven liquid crystal network actuators.

Soft Matter 2022 Jun 22. Epub 2022 Jun 22.

Département de Chimie, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Soft actuators based on liquid crystal networks (LCNs) have aroused great scientific interest for use as stimuli-controlled shape-changing and moving components for robotic devices due to their fast, large, programmable and solvent-free actuation responses. Recently, various LCN actuators have been implemented in soft robotics using stimulus sources such as heat, light, humidity and chemical reactions. Among them, electrically driven LCN actuators allow easy modulation and programming of the input electrical signals (amplitude, phase, and frequency) as well as stimulation throughout the volume, rendering them promising actuators for practical applications. Herein, the progress of electrically driven LCN actuators regarding their construction, actuation mechanisms, actuation performance, actuation programmability and the design strategies for intelligent systems is elucidated. We also discuss new robotic functions and advanced actuation control. Finally, an outlook is provided, highlighting the research challenges faced with this type of actuator.
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http://dx.doi.org/10.1039/d2sm00544aDOI Listing
June 2022

Activation effect of catechol on biotic and abiotic factors of humus formation during chicken manure composting.

Waste Manag 2022 Jun 18;149:146-155. Epub 2022 Jun 18.

College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

In this present study, catechol was added to promote humus formation in chicken manure composting using 12.5-L working volume lab-scale reactors. Chicken manure composting were carried out on two treatments, control (CK) and 0.5% (w/w) catechol treated group. Results showed that catechol addition reduced organic matter loss and improved the content of recalcitrant components. Humus and humic acid (HA) contents were, respectively, 69.2% and 82.3% higher in catechol treated-compost than in the control. With the addition of catechol, the bacterial community composition and richness was changed, e.g., the relative percentage of Bacillus and Sinibacillus were increased, which might contribute to humus and HA formation. Additionally, the activities of polyphenol oxidase and laccase (related to humus formation) were increased by the addition of catechol. Importantly, the catechol addition reduced the polyphenol content by 39.6% compared to control. Overall, the addition of catechol enhanced the biotic and abiotic factors to promote the humification process during composting, which might be a promising approach for accelerating the humification process and reducing contamination of phenolic compounds.
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http://dx.doi.org/10.1016/j.wasman.2022.06.014DOI Listing
June 2022

Inversion technique under fluoroscopy for removal of self-expanding nitinol esophageal stent after long-term placement: review of 107 consecutive cases.

Surg Endosc 2022 Jun 21. Epub 2022 Jun 21.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Background: Removal of self-expanding esophageal metal stents that have been implanted for a long time can be difficult and risky.

Purpose: In this paper, we describe the use of the "inversion technique" under fluoroscopy for removal of self-expandable nitinol esophageal stents that have been placed for long periods and evaluate the effectiveness and safety of the method.

Methods: Retrospective analysis of patients who underwent removal of self-expanding nitinol esophageal stents by the inversion technique under fluoroscopy at our center. Demographic characteristics, type of esophageal stents, stent retention time, reasons for stent removal, and related complications were collected from the case records and analyzed.

Results: A total of 112 metal esophageal stents (62 fully covered esophageal stents and 50 partially covered esophageal stents) were extracted from the 107 patients included in the study. Indications for stent implantation were malignant esophageal stenosis (27 patients), benign esophageal stenosis (42 patients), and esophageal fistula (38 patients). Median duration of stent retention was 77 days (29-727 days). All stents were removed successfully without major complications such as esophageal rupture, massive hemorrhage, asphyxia, or cardiorespiratory arrest.

Conclusion: Inversion technique under fluoroscopy appears to be a safe, effective, and quick procedure for removal of self-expanding nitinol esophageal stent after long-term placement.
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http://dx.doi.org/10.1007/s00464-022-09238-2DOI Listing
June 2022

Genipin protects against mitochondrial damage of the retinal pigment epithelium under hyperglycemia through the pathway mediated by the signaling axis.

Ann Transl Med 2022 May;10(10):587

College of Medical Technology, Qiqihar Medical University, Qiqihar, China.

Background: To investigate the protective effect and mechanism of genipin (GE) on mitochondrial damage in retinal pigment epithelial (RPE) cells induced by high glucose.

Methods: Differential genes of GE in the treatment of diabetic retinopathy (DR) were screened by the Gene Expression Omnibus (GEO) database. Differential genes located in the pathway were obtained. TargetScan, miRDB, and DIANA databases were used to predict the targeted microRNAs (miRNAs) of differential genes. A high-fat diet combined with streptomycin (STZ) intraperitoneal injection were used to establish a diabetic mouse model. Diabetic mice were treated with GE by intragastric administration. The functional and molecular changes of the retina were detected by electroretinogram (ERG) and reverse transcription-polymerase chain reaction (RT-PCR). ARPE-19 cells were cultured under hyperglycemic conditions with and inhibitors. was knocked down/overexpressed to detect changes in cell function, activity, and mitochondrial function. The dual luciferase reporter assay confirmed the targeted binding of with

Results: Bioinformatics analysis finally yielded as the research target gene. was predicted to be the targeted miRNA of by online databases. The results of animal experiments showed that the retinal function of mice recovered after GE administration (P<0.05), the expression of and in RPE cells was significantly increased (P<0.05), and the expression of was significantly decreased (P<0.05). The results of cell experiments showed that the functions of cells and mitochondria recovered after the addition of GE under hyperglycemia (P<0.05). Cell and mitochondrial functions were decreased after the addition of inhibitor (P<0.05). Overexpression of or inhibition of increased cell activity and mitochondrial function (P<0.05). The results of the dual luciferase reporter assay showed that had a targeted binding site with

Conclusions: GE protects ARPE-19 cell functional activity, inflammatory responses, and mitochondrial damage by promoting the signaling pathway and regulating the expression of the / signaling axis.
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http://dx.doi.org/10.21037/atm-22-2219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201119PMC
May 2022

Abnormal Activation of Tryptophan-Kynurenine Pathway in Women With Polycystic Ovary Syndrome.

Front Endocrinol (Lausanne) 2022 1;13:877807. Epub 2022 Jun 1.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Background: Women with polycystic ovary syndrome (PCOS) suffer from dysfunctional metabolism and studies have reported increased levels of tryptophan in patients with PCOS. However, the changes of downstream metabolites in tryptophan catabolism pathways remain unclear.

Methods: This is a cross-sectional study that included 200 PCOS patients and 200 control women who were recruited from the Reproductive Medicine Center of Peking University Third Hospital from October 2017 to June 2019. The PCOS patients and the control group were further divided into subtypes of normal weight and overweight/obesity. Fasting blood samples from all subjects were collected on days 2~3 of a natural menstrual cycle or when amenorrhea for over 40 days with follicle diameter not exceeding 10 mm. The plasma levels of tryptophan metabolites were quantitatively determined by the liquid chromatograph mass spectrometer, including tryptophan, serotonin, kynurenine, kynurenic acid, 3-hydroxykynurenine, and quinolinic acid.

Results: The tryptophan-kynurenine pathway was dysregulated in women with PCOS, along with significantly elevated levels of tryptophan, serotonin, kynurenine, kynurenic acid, and quinolinic acid. Moreover, levels of tryptophan, kynurenine, and kynurenic acid were positively correlated with luteinizing hormone, anti-Müllerian hormone, fasting insulin, HOMA-IR. tryptophan, and kynurenine and quinolinic acid had an obvious association with C-reactive protein levels. Furthermore, logistic regression showed that tryptophan, serotonin, kynurenine, kynurenic acid and quinolinic acid were all associated significantly with the increased risk of PCOS with the adjustment for potential confounding factors. Additionally, tryptophan, kynurenine, and kynurenic acid had good diagnostic performances for PCOS, and their combination exhibited higher sensitivity and specificity to diagnostic efficiency, with the area under the ROC curve of 0.824 (95% CI 0.777-0.871), which was comparable to the endocrine indicators.

Conclusion S: The tryptophan-kynurenine pathway was abnormally activated in PCOS patients.
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http://dx.doi.org/10.3389/fendo.2022.877807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199373PMC
June 2022

Development and Characterization of - 6S Recombinants Harboring a Novel Powdery Mildew Resistance Gene .

Front Plant Sci 2022 2;13:918508. Epub 2022 Jun 2.

State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.

Powdery mildew of wheat is a foliar disease that is spread worldwide. Cultivation of resistant varieties is the most effective, economical, and environmentally friendly strategy to curb this disease. Powdery mildew resistance genes () are the primary resources for resistance breeding, and new genes are in constant demand. Previously, we identified chromosome 6S#3 as a carrier of powdery mildew resistance and designated the resistance gene as . Here, we reported the design of 24 markers specific to 6S#3 on the basis of the full-length cDNA sequences of 6S#3 donor . accession TA1910, and the development of wheat- 6S#3 introgression stocks by induced homoeologous recombination. Further, 6S#3 introgression lines were identified and characterized by integration analysis of powdery mildew responses, hybridization, and molecular markers and was mapped to a distal interval of 42.80 Mb between markers Ael58410 and Ael57699 in the long arm of 6S#3. Two resistant recombinants, R43 (T6BS.6BL-6S#3L) and T27 (Ti6AS.6AL-6S#3L-6AL), contained segments harboring with less than 8% of 6S#3 genomic length, and two markers were diagnostic for . This study broadened powdery mildew resistance gene resources for wheat improvement and provided a fundamental basis for fine mapping and cloning of to further understand its molecular mechanism of disease resistance.
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http://dx.doi.org/10.3389/fpls.2022.918508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201914PMC
June 2022

Nanoplastics cause transgenerational toxicity through inhibiting germline microRNA mir-38 in C. elegans.

J Hazard Mater 2022 Jun 6;437:129302. Epub 2022 Jun 6.

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen 518122, China. Electronic address:

Nanoplastic exposure potentially caused the induction of transgenerational toxicity. Nevertheless, the molecular basis for nanoplastic exposure-induced transgenerational toxicity remains largely unclear. Using Caenorhabditis elegans as an animal model, we examined the role of germline microRNA (miRNA) mir-38 in regulating the transgenerational toxicity of polystyrene nanoparticles (PS-NPs). After the exposure, 1-100 μg/L PS-NP decreased expression of germline mir-38. Meanwhile, germline mir-38 overexpression conferred a resistance to transgenerational PS-NP toxicity, which suggested that the decrease in germline mir-38 mediated the induction of transgenerational PS-NP toxicity. In the germline, mir-38 regulated transgenerational PS-NP toxicity by inhibiting activity of downstream targets (NDK-1, NHL-2, and WRT-3). Among these three downstream targets, germline NDK-1 further controlled transgenerational PS-NP toxicity by suppressing the function of KSR-1/2, two kinase suppressors of Ras. Therefore, in the germline, the decrease in mir-38 mediated induction of transgenerational PS-NP toxicity by at least inhibiting signaling cascade of NDK-1-KSR-1/2 in nematodes. The findings in this study are helpful for providing relevantly molecular endpoints to assess potential transgenerational toxicity of nanoplastics.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129302DOI Listing
June 2022

Two-dimensional free space electric field imaging using electric field induced second harmonic generation.

Authors:
Yue Zhao Takao Fuji

Opt Lett 2022 Jun;47(12):2999-3002

We present a new, to the best of our knowledge, approach for the measurement of the localized electric field distribution in air using electric field induced second harmonic generation combined with a microscopic imaging technique. This method only needs two snapshot second harmonic images with orthogonal polarizations to obtain the two-dimensional spatial distribution of the intensity and direction of the electric field. The distribution of a local electric field was clearly measured with a spatial resolution of 8.8 µm by using this method. The measurement of a single second harmonic image takes 5 s by using a 5 kHz repetition rate femtosecond laser.
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http://dx.doi.org/10.1364/OL.460742DOI Listing
June 2022

Wheat heat shock factor TaHsfA2d contributes to plant responses to phosphate deficiency.

Plant Physiol Biochem 2022 Jun 8;185:178-187. Epub 2022 Jun 8.

College of Life Sciences/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

Phosphate (Pi) availability has become a major constraint limiting crop growth and production. Heat shock factors (Hsfs) play important roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. However, whether members of the Hsf family are involved in the transcriptional regulation of plant responses to Pi insufficiency has not been reported. Here, we identified that TaHsfA2d, a member of the heat shock factor family, was strongly repressed by Pi deficiency. Overexpressing TaHsfA2d-4A in Arabidopsis results in significantly enhanced sensitivity to Pi deficiency, evidenced by increased anthocyanin content, decreased proliferation and elongation of lateral roots, and reduced Pi uptake. Furthermore, RNA-seq analyses showed that TaHsfA2d-4A functions through up-regulation of a number of genes involved in stress responses and flavonoid biosynthesis. Collectively, these results provide evidence that TaHsfA2d participates in the regulation of Pi deficiency stress, and that TaHsfA2d could serve as a valuable gene for genetic modification of crop tolerance to Pi starvation.
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http://dx.doi.org/10.1016/j.plaphy.2022.05.035DOI Listing
June 2022

Effectiveness of Proanthocyanidin plus Trimetazidine in the Treatment of Non-Small-Cell Lung Cancer with Radiation Heart Injury.

Evid Based Complement Alternat Med 2022 3;2022:2338622. Epub 2022 Jun 3.

Ward I, Department of Cardiovascular Medicine, Cangzhou People's Hospital, Cangzhou, China.

This study was intended to explore the effect of proanthocyanidin (PC) combined with trimetazidine in non-small-cell lung cancer (NSCLC) with radiation-induced heart damage (RIHD). It was a prospective randomized controlled study that 86 NSCLC patients with radiation treatment in Cangzhou People's Hospital from January 2019 and June 2021 were enrolled and randomized to either the control group or the study group via the random table method, 43 cases in each group. The control group received trimetazidine, and the study group additionally received PC. The incidence of RIHD-related clinical manifestation, RIHD-related ECG, and RIHD-related cardiac ultrasound change were all lower in the study group. After radiotherapy, the serum level of superoxide dismutase (SOD) was higher, and malondialdehyde (MDA) was lower in the study group when compared with the control group. After radiotherapy, the serum levels of brain natriuretic peptide (BNP), cardiac troponin (cTnT), creatine kinase (CK), and creatine kinase isoenzymes (CKMB) were all lower in the study group when compared with the control group. The efficacy of PC plus trimetazidine for NSCLC with RIHD is superior to trimetazidine alone, and it significantly mitigates radiation-induced inflammatory response and oxidative stress.
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http://dx.doi.org/10.1155/2022/2338622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187471PMC
June 2022

Prognostic value of patient-reported outcomes in predicting 30 day all-cause readmission among older patients with heart failure.

ESC Heart Fail 2022 Jun 9. Epub 2022 Jun 9.

School of Nursing, Tianjin Medical University, Tianjin, China.

Aims: Previous prediction studies for 30 day readmission in patients with heart failure were built mainly based on electronic medical records and rarely involved patient-reported outcomes. This study aims to develop and validate a nomogram including patient-reported outcomes to predict the possibility of 30 day all-cause readmission in older patients with heart failure and to explore the value of patient-reported outcomes in prediction model.

Methods And Results: This was a prospective cohort study. The nomogram was developed and internally validated by Logistic regression analysis based on 381 patients in training group from March to December 2019. The nomogram was externally validated based on 170 patients from July to October 2020. Receiver operating characteristic curves, calibration plots and decision-curve analysis were used to evaluate the performance of the nomogram. A total of 381 patients' complete data were analysed in the training group and 170 patients were enrolled in the external validation group. In the training group, 14.4% (n = 55) patients were readmitted to hospitals within 30 days of discharge and 15.9% (n = 27) patients were readmitted in the external validation group. The nomogram included six factors: history of surgery, changing the type of medicine by oneself, information acquisition ability, subjective support, depression level, quality of life, all of which were significantly associated with 30 day readmission in older patients with heart failure. The areas under the receiver operating characteristic curves of nomogram were 0.949 (95% CI: 0.925, 0.973, sensitivity: 0.873, specificity: 0.883) and 0.804 (95% CI: 0.691, 0.917, sensitivity: 0.778, specificity: 0.832) respectively in the training and external validation groups, which indicated that the nomogram had better discrimination ability. The calibration plots demonstrated favourable coordination between predictive probability of 30 day readmission and observed probability. Decision-curve analysis showed that the net benefit of the nomogram was better between threshold probabilities of 0-85%.

Conclusions: A novel and easy-to-use nomogram is constructed and demonstrated which emphasizes the important role of patient-reported outcomes in predicting studies. The performance of the nomogram drops in the external validation cohort and the nomogram must be validated in a wide prospective cohort of HF patients before its clinical relevance can be demonstrated. All these findings in this study can assist professionals in identifying the needs of HF patients so as to reduce 30 day readmission.
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http://dx.doi.org/10.1002/ehf2.13991DOI Listing
June 2022

Long-Term High-Fat High-Fructose Diet Induces Type 2 Diabetes in Rats through Oxidative Stress.

Nutrients 2022 May 24;14(11). Epub 2022 May 24.

The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology of National Health Commission, Beijing 100730, China.

Long-term consumption of a Western diet is a major cause of type 2 diabetes mellitus (T2DM). However, the effects of diet on pancreatic structure and function remain unclear. Rats fed a high-fat, high-fructose (HFHF) diet were compared with rats fed a normal diet for 3 and 18 months. Plasma biochemical parameters and inflammatory factors were used to reflect metabolic profile and inflammatory status. The rats developed metabolic disorders, and the size of the islets in the pancreas increased after 3 months of HFHF treatment but decreased and became irregular after 18 months. Fasting insulin, C-peptide, proinsulin, and intact proinsulin levels were significantly higher in the HFHF group than those in the age-matched controls. Plasmatic oxidative parameters and nucleic acid oxidation markers (8-oxo-Gsn and 8-oxo-dGsn) became elevated before inflammatory factors, suggesting that the HFHF diet increased the degree of oxidative stress before affecting inflammation. Single-cell RNA sequencing also verified that the transcriptional level of oxidoreductase changed differently in islet subpopulations with aging and long-term HFHF diet. We demonstrated that long-term HFHF diet and aging-associated structural and transcriptomic changes that underlie pancreatic islet functional decay is a possible underlying mechanism of T2DM, and our study could provide new insights to prevent the development of diet-induced T2DM.
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http://dx.doi.org/10.3390/nu14112181DOI Listing
May 2022

Dosage Effect of Wet-Process Tuff Silt Powder as an Alternative Material of Sand on the Performance of Reactive Powder Concrete.

Materials (Basel) 2022 May 31;15(11). Epub 2022 May 31.

Beijing Zhonglu Gaoke Highway Technology Co., Ltd., Beijing 100088, China.

A large amount of stone powder is produced during the production of machine-made sand. This research aims to study the effect of wet-process tuff silt powder (WTSP) dosages (as an alternative sand material to utilize waste stone powder and reduce environmental hazards) on reactive powder concrete's (RPC) mechanical performance. The physical and chemical properties of WTSP were analyzed as per relevant standards. This study prepared RPC samples with various WTSP content (0%, 6%, 12%, and 18%) to replace quartz sand at the same water-binder ratio (0.14) and allowed the samples to cure for 3 days, 7 days and 28 days prior to unconfined compression testing and flexural testing. Scanning electron microscopy (SEM) and Mercury Intrusion Porosimetry (MIP) testing were also carried out to observe the evolution of macroscopic properties in response to replacing part of quartz sand with the same amount of WTSP. The results show that the developed flexural and unconfined compressive strength (UCS) decreases slowly with a greater dosage of WTSP. However, when the WTSP content is 12% or less, the RPC made with WTSP satisfies the industrial application threshold regarding mechanical properties. For RPC samples containing more than 12% WTSP, the UCS and flexural strength showed a dramatic drop. Thus 12% of WTSP content was deemed the maximum and the corresponding UCS of 104.6 MPa and flexural strength of 12 MPa for 28 days of curing were the optimums. The microscopic characteristics indicate that the addition of WTSP can effectively fill the large pores in the RPC micro-structure, hence reducing the porosity of RPC. Furthermore, the WTSP can react with the cementitious material to form calcium aluminate during the hydration process, further strengthening the interface. The alkaline calcium carbonate in WTSP could improve the interfacial adhesion and make the structure stronger.
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http://dx.doi.org/10.3390/ma15113930DOI Listing
May 2022

Investigating Biochar-Derived Dissolved Organic Carbon (DOC) Components Extracted Using a Sequential Extraction Protocol.

Materials (Basel) 2022 May 28;15(11). Epub 2022 May 28.

Gansu Dust Suppression for Transportation and Storage Engineering Research Center, Lanzhou 730000, China.

Biochar-derived dissolved organic carbon (DOC), as the most important component of biochar, can be released on farmland, improving fertility and playing a role in soil amendment and remediation. The complexity of molecular structures and diversity of DOC compounds have influenced these functions to some extent. A sequential extract protocol consisting of water (25 °C), hot water (80 °C), and NaOH solution (0.05 M) was used to fully extract DOC compounds and gain a thorough understanding of the possible DOC components released from biochar. Rape straw (RS), apple tree branches (ATB), and pine sawdust (PS) were pyrolyzed at 300, 500, and 700 °C, respectively, to make nine distinct biochars. A TOC analyser, ultraviolet-visible spectroscopy (UV-vis), and excitation-emission fluorescence (EEM) spectrophotometer were used in conjunction with parallel factor analysis (PARAFAC) to determine the distribution of DOC content, the diversity of aromaticity, molecular weight characteristics and components of biochar-derived DOC. The results show that the relative distribution of water-extractable fractions ranged from 3.21 to 35.57%, with a low-aromaticity and extremely hydrophilic fulvic-acid-like compounds being found in the highest amounts (C2 and C3). The smallest amount of hot water-extractable components was produced from the release of small-molecule aliphatic compounds adsorbed on biochar and susceptible to migration loss once in a soil solution. More than half of the biochar-derived DOC was released in a NaOH solution, which primarily consisted of humic-acid-like compounds (C1), with higher molecular weights, more aromaticity, and lower bioavailability, according to the distribution of DOC in various extractants. In addition, the pyrolysis temperature and biomass type had a significant impact on the DOC properties released by biochar. As a result, the findings of this study showed that using a sequential extract protocol of water, hot water, and NaOH solution in combination with spectroscopic methods could successfully reveal the diversity of biochar-derived components, which could lead to new insights for the accurate assessment of potential environmental impacts and new directions for biochar applications.
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http://dx.doi.org/10.3390/ma15113865DOI Listing
May 2022

Quantitative Response of Gray-Level Co-Occurrence Matrix Texture Features to the Salinity of Cracked Soda Saline-Alkali Soil.

Int J Environ Res Public Health 2022 May 27;19(11). Epub 2022 May 27.

Heilongjiang Province Key Laboratory of Geographical Environment Monitoring and Spatial Information Service in Cold Regions, Harbin Normal University, Harbin 150025, China.

Desiccation cracking during water evaporation is a common phenomenon in soda saline-alkali soils and is mainly determined by soil salinity. Therefore, quantitative measurement of the surface cracking status of soda saline-alkali soils is highly significant in different applications. Texture features can help to determine the mechanical properties of soda saline-alkali soils, thus improving the understanding of the mechanism of desiccation cracking in saline-alkali soils. This study aims to provide a new standard describing the surface cracking conditions of soda saline-alkali soil on the basis of gray-level co-occurrence matrix (GLCM) texture analysis and to quantitatively study the responses of GLCM texture features to soil salinity. To achieve this, images of 200 field soil samples with different surface cracks were processed and calculated for GLCMs under different parameters, including directions, gray levels, and step sizes. Subsequently, correlation analysis was then conducted between texture features and electrical conductivity (EC) values. The results indicated that direction had little effect on the GLCM texture features, and that four selected texture features, contrast (CON), angular second moment (ASM), entropy (ENT), and homogeneity (HOM), were the most correlated with EC under a gray level of 2 and step size of 1 pixel. The results also showed that logarithmic models can be used to accurately describe the relationships between EC values and GLCM texture features of soda saline-alkali soils in the Songnen Plain of China, with calibration R ranging from 0.88 to 0.92, and RMSE from 2.12 × 10 to 9.68 × 10, respectively. This study can therefore enhance the understanding of desiccation cracking of salt-affected soil to a certain extent and can also help to improve the detection accuracy of soil salinity.
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http://dx.doi.org/10.3390/ijerph19116556DOI Listing
May 2022

Rational Design of P450 aMOx for Improving Anti-Markovnikov Selectivity Based on the "Butterfly" Model.

Front Mol Biosci 2022 23;9:888721. Epub 2022 May 23.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, Shanghai Key Laboratory of Green Chemistry and Chemical Process, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.

Aromatic aldehydes are important industrial raw materials mainly synthesized by anti-Markovnikov (AM) oxidation of corresponding aromatic olefins. The AM product selectivity remains a big challenge. P450 aMOx is the first reported enzyme that could catalyze AM oxidation of aromatic olefins. Here, we reported a rational design strategy based on the "butterfly" model of the active site of P450 aMOx. Constrained molecular dynamic simulations and a binding energy analysis of key residuals combined with an experimental alanine scan were applied. As a result, the mutant A275G showed high AM selectivity of >99%. The results also proved that the "butterfly" model is an effective design strategy for enzymes.
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http://dx.doi.org/10.3389/fmolb.2022.888721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168652PMC
May 2022

Preparation and quality evaluation of Honokiol nanoparticles using a new polysaccharide polymer as its carrier.

Curr Drug Deliv 2022 Jun 7. Epub 2022 Jun 7.

School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China.

Objective: To improve solubility of Honokiol (HNK), Honokiol nanoparticles (HNK-NPs) were prepared by using a new biodegradable polysaccharide polymer as its carrier.

Methods: HNK-NPs were prepared by hydrophilic polymer coagulation method, and the processing parameters were optimized according to average particle size and PDI by single factor experiment. The morphology of the optimized nanoparticles was investigated by TEM and the in vitro release was carried out to evaluate the optimized HNK-NPs.

Results: The encapsulation efficiency and drug loading of the HNK-NPs were 77.75 ± 2.63% and 13.46 ± 0.39%. The obtained nanoparticles of HNK-NPs were spherical-like under the electron microscope with a mean particle size of 198.50 ± 0.01 nm and Zeta potential of -52.60 ± 1.00 mV, respectively. The in vitro release results showed that the cumulative release rates of nanoparticles were 48.28 ± 9.80% and 81.12 ± 4.35% within 2 h and 8 h, respectively which showed a stable release behavior. The average particle size and PDI of HNK-NPs solution prepared by hydrophilic polymer condensation method had no obvious change at 72h.

Conclusion: HNK-NPs were successfully prepared by phase separation method. This new polysaccharide polymer should be an ideal carrier to help improving the solubility of HNK.
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http://dx.doi.org/10.2174/1567201819666220607153457DOI Listing
June 2022

Wnt4 is crucial for cardiac repair by regulating mesenchymal-endothelial transition via the phospho-JNK/JNK.

Theranostics 2022 13;12(9):4110-4126. Epub 2022 May 13.

Heart Center and Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510120, China.

Wnt4 plays a critical role in development and is reactivated during fibrotic injury; however, the role of Wnt4 in cardiac repair remains unclear. In this study, our aim was to clarify the pathophysiological role and mechanisms of Wnt4 following acute cardiac ischemic reperfusion injury. We investigated the spatio-temporal expression of Wnt4 following acute cardiac ischemic reperfusion injury and found that Wnt4 was upregulated as an early injury response gene in cardiac fibroblasts near the injury border zone and associated with mesenchymal-endothelial transition (MEndoT), a beneficial process for revascularizing the damaged myocardium in cardiac repair. Using ChIP assay and and loss- and gain-of-function, we demonstrated that Wnt4 served as a crucial downstream target gene of p53 during MEndoT. Wnt4 knockdown in cardiac fibroblasts led to decreased MEndoT and worsened cardiac function. Conversely, Wnt4 overexpression in cardiac fibroblasts induced MEndoT in these cells via the phospho-JNK/JNK signaling pathway; however, both the p53 and Wnt4 protein levels were dependent on the β-catenin signaling pathway. JNK activation plays a critical role in the induction of MEndoT and is crucial for Wnt4 regulated MEndoT. Moreover, Wnt4 overexpression specifically in cardiac fibroblasts rescued the cardiac function worsening due to genetic p53 deletion by decreasing fibrosis and increasing MEndoT and vascular density. Our study revealed that Wnt4 plays a pivotal role in cardiac repair with involvement of phospho-JNK mediated MEndoT and is a crucial gene for cardiac fibroblast-targeted therapy in heart disease.
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http://dx.doi.org/10.7150/thno.71392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169355PMC
June 2022

Association of Dietary Phytosterols Intake and Survival of Esophageal Squamous Cell Carcinoma: A Prospective Cohort Study.

Nutr Cancer 2022 Jun 7:1-10. Epub 2022 Jun 7.

School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China.

Background: The association of dietary phytosterols intake with survival of esophageal squamous cell carcinoma (ESCC) remains unclear. This study was to examine the effect of dietary phytosterols intake on ESCC survival in a Chinese rural population.

Methods: A total of 942 incident ESCC patients diagnosed between 2011 and 2013 in Yanting area were followed up until March 1, 2020. Dietary intake five years before ESCC diagnosis was collected using a food frequency questionnaire. The outcome of interest was all-cause mortality. Cox proportional hazards regression model was used to estimate hazard ratio (HR) and 95% confidence intervals (CI).

Results: When comparing the highest with lowest intake quartiles, intake of five specific and total phytosterols was not significantly associated with risk of death after adjustment for covariates, the adjusted HR (95% CI) for β-sitosterol, campesterol, stigmasterol, β-sitostanol, campestanol and total phytosterols was 0.90 (95% CI: 0.70-1.16), 0.92 (95% CI: 0.71-1.19), 0.86 (95% CI: 0.66-1.12), 0.93 (95% CI: 0.73-1.20), 0.94 (95% CI: 0.72-1.21), 0.89 (95% CI: 0.69-1.15), respectively.

Conclusion: This study does not find any association between pre-diagnostic phytosterols intake and risk of all-cause mortality among ESCC patients. Further research is required to determine the effect of post-diagnostic phytosterols intake on ESCC survival.
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http://dx.doi.org/10.1080/01635581.2022.2085311DOI Listing
June 2022

Synthesis and Characterization of A Stable Extra-Large-Pore Zeolite with 15×12×12 Member-Ring Channels.

Chemistry 2022 Jun 6. Epub 2022 Jun 6.

Nanjing University, School of Chemistry and Chemical Engineering, 22 Hankou Road, 210093, Nanjing, CHINA.

Extra-large-pore zeolites have great application potential in various industrial fields, such as oil refinery, fine chemicals and biomass processing. Herein, we report the synthesis of an extra-large-pore germanosilicate zeolite (named NUD-13) by using an easily obtained aromatic organic cation 1,2-dimethyl-3-propyl-benzimidazolium as organic structure-directing agents. NUD-13 possesses a rare 15-member ring extra-large-pore channel intersecting with two elliptical 12-member ring channels, which is isostructural to germanosilicate zeolite GeZA synthesized by using triphenylsulfonium. The germanium in NUD-13 can be partially substituted by acid treatment to obtain stable high silica zeolite. In addition, aluminium is added into the framework of NUD-13 during the post-synthesis treatment process, which provides a foundation for catalytic application.
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http://dx.doi.org/10.1002/chem.202200934DOI Listing
June 2022

Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.

Pharmacol Res 2022 May 31;182:106284. Epub 2022 May 31.

Department of Pharmacology, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin 150086, China; Research Unit of Noninfectious Chronic Diseases in Frigid Zone, Chinese Academy of Medical Sciences, 2019RU070, China; NHC Key Laboratory of Cell Transplantation, Harbin Medical University, Harbin, China. Electronic address:

Pathological cardiac hypertrophy is a process characterized by significant disturbance of protein turnover. Cullin-associated and Neddylation-dissociated 1 (CAND1) acts as a coordinator to modulate substrate protein degradation by promoting the formation of specific cullin-based ubiquitin ligase 3 complex in response to substrate accumulation, which thereby facilitate the maintaining of normal protein homeostasis. Accumulation of calcineurin is critical in the pathogenesis of cardiac hypertrophy and heart failure. However, whether CAND1 titrates the degradation of hypertrophy related protein eg. calcineurin and regulates cardiac hypertrophy remains unknown. Therefore, we aim to explore the role of CAND1 in cardiac hypertrophy and heart failure and the underlying molecular mechanism. Here, we found that the protein level of CAND1 was increased in cardiac tissues from heart failure (HF) patients and TAC mice, whereas the mRNA level did not change. CAND1-KO+ /- aggravated TAC-induced cardiac hypertrophic phenotypes; in contrast, CAND1-Tg attenuated the maladaptive cardiac remodeling. At the molecular level, CAND1 overexpression downregulated, whereas CAND1-KO+ /- or knockdown upregulated calcineurin expression at both in vivo and in vitro conditions. Mechanistically, CAND1 overexpression favored the assembly of Cul1/atrogin1/calcineurin complex and rendered the ubiquitination and degradation of calcineurin. Notably, CAND1 deficiency-induced hypertrophic phenotypes were partially rescued by knockdown of calcineurin, and application of exogenous CAND1 prevented TAC-induced cardiac hypertrophy. Taken together, our findings demonstrate that CAND1 exerts a protective effect against cardiac hypertrophy and heart failure partially by inducing the degradation of calcineurin.
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http://dx.doi.org/10.1016/j.phrs.2022.106284DOI Listing
May 2022

Characteristics of the cancer stem cell niche and therapeutic strategies.

Stem Cell Res Ther 2022 Jun 3;13(1):233. Epub 2022 Jun 3.

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Shanghai, 200032, China.

Distinct regions harboring cancer stem cells (CSCs) have been identified within the microenvironment of various tumors, and as in the case of their healthy counterparts, these anatomical regions are termed "niche." Thus far, a large volume of studies have shown that CSC niches take part in the maintenance, regulation of renewal, differentiation and plasticity of CSCs. In this review, we summarize and discuss the latest findings regarding CSC niche morphology, physical terrain, main signaling pathways and interactions within them. The cellular and molecular components of CSCs also involve genetic and epigenetic modulations that mediate and support their maintenance, ultimately leading to cancer progression. It suggests that the crosstalk between CSCs and their niche plays an important role regarding therapy resistance and recurrence. In addition, we updated diverse therapeutic strategies in different cancers in basic research and clinical trials in this review. Understanding the complex heterogeneity of CSC niches is a necessary pre-requisite for designing superior therapeutic strategies to target CSC-specific factors and/or components of the CSC niche.
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http://dx.doi.org/10.1186/s13287-022-02904-1DOI Listing
June 2022

Multiple Traffic Target Tracking with Spatial-Temporal Affinity Network.

Comput Intell Neurosci 2022 23;2022:9693767. Epub 2022 May 23.

Shaanxi Transportation Holding Group Co., Ltd., Xi'an 710000, China.

Traffic target tracking is a core task in intelligent transportation system because it is useful for scene understanding and vehicle autonomous driving. Most state-of-the-art (SOTA) multiple object tracking (MOT) methods adopt a two-step procedure: object detection followed by data association. The object detection has made great progress with the development of deep learning. However, the data association still heavily depends on hand crafted constraints, such as appearance, shape, and motion, which need to be elaborately trained for a special object. In this study, a spatial-temporal encoder-decoder affinity network is proposed for multiple traffic targets tracking, aiming to utilize the power of deep learning to learn a robust spatial-temporal affinity feature of the detections and tracklets for data association. The proposed spatial-temporal affinity network contains a two-stage transformer encoder module to encode the features of the detections and the tracked targets at the image level and the tracklet level, aiming to capture the spatial correlation and temporal history information. Then, a spatial transformer decoder module is designed to compute the association affinity, where the results from the two-stage transformer encoder module are fed back to fully capture and encode the spatial and temporal information from the detections and the tracklets of the tracked targets. Thus, efficient affinity computation can be applied to perform data association in online tracking. To validate the effectiveness of the proposed method, three popular multiple traffic target tracking datasets, KITTI, UA-DETRAC, and VisDrone, are used for evaluation. On the KITTI dataset, the proposed method is compared with 15 SOTA methods and achieves 86.9% multiple object tracking accuracy (MOTA) and 85.71% multiple object tracking precision (MOTP). On the UA-DETRAC dataset, 12 SOTA methods are used to compare with the proposed method, and the proposed method achieves 20.82% MOTA and 35.65% MOTP, respectively. On the VisDrone dataset, the proposed method is compared with 10 SOTA trackers and achieves 40.5% MOTA and 74.1% MOTP, respectively. All those experimental results show that the proposed method is competitive to the state-of-the-art methods by obtaining superior tracking performance.
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http://dx.doi.org/10.1155/2022/9693767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152393PMC
June 2022

ERK-Smurf1-RhoA signaling is critical for TGFβ-drived EMT and tumor metastasis.

Life Sci Alliance 2022 Oct 2;5(10). Epub 2022 Jun 2.

The Central Lab of Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China

Epithelial-mesenchymal transition (EMT) has fundamental roles in various biological processes. However, there are still questions pending in this fast-moving field. Here we report that in TGFβ-induced EMT, ERK-mediated Smurf1 phosphorylation is a prerequisite step for RhoA degradation and the consequent mesenchymal state achievement. Upon TGFβ treatment, activated ERK phosphorylates Thr223 of Smurf1, a member of HECT family E3 ligase, to promote Smurf1-mediated polyubiquitination and degradation of RhoA, thereby leading to cell skeleton rearrangement and EMT. Blockade of phosphorylation of Smurf1 inhibits TGFβ-induced EMT, and accordingly, dramatically blocks lung metastasis of murine breast cancer in mice. Hence, our study reveals an unknown role of ERK in TGFβ-induced EMT and points out a potential strategy in therapeutic intervention.
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http://dx.doi.org/10.26508/lsa.202101330DOI Listing
October 2022

Spatial and Temporal Transcriptomic Heredity and Asymmetry in an Artificially Constructed Allotetraploid Wheat (AADD).

Front Plant Sci 2022 16;13:887133. Epub 2022 May 16.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, China.

Polyploidy, or whole-genome duplication (WGD), often induces dramatic changes in gene expression due to "transcriptome shock. " However, questions remain about how allopolyploidy (the merging of multiple nuclear genomes in the same nucleus) affects gene expression within and across multiple tissues and developmental stages during the initial foundation of allopolyploid plants. Here, we systematically investigated the immediate effect of allopolyploidy on gene expression variation in an artificial allopolyploidy system consisting of a constructed allotetraploid wheat (AADD genome, accession AT2) and its diploid progenitors and . We performed comprehensive RNA sequencing of 81 samples from different genotypes, tissues, and developmental stages. First, we found that intrinsic interspecific differences between the diploid parents played a major role in establishing the expression architecture of the allopolyploid. Nonetheless, allopolyploidy also induced dramatic and asymmetric patterns of differential gene expression between the subgenomes, and genes from the D subgenome exhibited a more drastic response. Second, analysis of homoeolog expression bias (HEB) revealed that the D subgenome exhibited significant expression bias and that -generated HEB was attributed mainly to asymmetrical differential gene expression. Homoeolog-specific expression (HSE) analyses showed that the -only regulatory pattern was predominant in AT2, reflecting significant divergence between the parents. Co-expression network analysis revealed that homoeolog expression connectivity (HEC) was significantly correlated with sequence divergence in elements between subgenomes. Interestingly, allopolyploidy-induced reconstruction of network modules was also associated with different HSE patterns. Finally, a transcriptome atlas of spike development demonstrated that the phenotypic similarity of AT2 to may be attributed to the combination of relatively stable expression of A-subgenome genes and drastic downregulation of their D-subgenome homoeologs. These findings provide a broad, multidimensional characterization of allopolyploidy-induced transcriptomic responses and suggest that allopolyploidy can have immediate and complex regulatory effects on the expression of nuclear genes.
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http://dx.doi.org/10.3389/fpls.2022.887133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150853PMC
May 2022

Asymmetrically Doping a Platinum Atom into a Au Nanocluster for Changing the Electron Configuration and Reactivity in Electrocatalysis.

Angew Chem Int Ed Engl 2022 May 30:e202207685. Epub 2022 May 30.

Key Lab of Mesoscopic Chemistry of MOE and Jiangsu Key Lab of Vehicle Emissions Control, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.

It is an obstacle to precisely manipulate a doped heteroatom into a desired position in a metal nanocluster. Herein, we overcome this difficulty to obtain Pt Au (SCH Ph Bu) and Pt Au (SCH Ph Bu) nanoclusters via controllably doping Pt atoms into the kernels of Au (SCH Ph Bu) . We reveal that asymmetrical doping of one Pt atom into either of the cores of Au (SCH Ph Bu) elevates the relative energy of the HOMO (highest occupied molecular orbital) accompanied by one valence electron loss of Pt Au (SCH Ph Bu) , compared to Au (SCH Ph Bu) with 14 electrons, while symmetrical doping of two Pt atoms into the cores of Au (SCH Ph Bu) narrows the HOMO-LUMO gap (LUMO: lowest unoccupied molecular orbital) of Pt Au (SCH Ph Bu) with two valence electrons less. Consequently, Pt Au (SCH Ph Bu) shows an electron-spin-induced high activity for CO electroreduction, whereas Pt Au (SCH Ph Bu) is least efficient and Au (SCH Ph Bu) has a decent performance.
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http://dx.doi.org/10.1002/anie.202207685DOI Listing
May 2022

Semantic Graph Attention with Explicit Anatomical Association Modeling for Tooth Segmentation from CBCT Images.

IEEE Trans Med Imaging 2022 May 30;PP. Epub 2022 May 30.

Accurate tooth identification and delineation in dental CBCT images are essential in clinical oral diagnosis and treatment. Teeth are positioned in the alveolar bone in a particular order, featuring similar appearances across adjacent and bilaterally symmetric teeth. However, existing tooth segmentation methods ignored such specific anatomical topology, which hampers the segmentation accuracy. Here we propose a semantic graph-based method to explicitly model the spatial associations between different anatomical targets (i.e., teeth) for their precise delineation in a coarse-to-fine fashion. First, to efficiently control the bilaterally symmetric confusion in segmentation, we employ a lightweight network to roughly separate teeth as four quadrants. Then, designing a semantic graph attention mechanism to explicitly model the anatomical topology of the teeth in each quadrant, based on which voxel-wise discriminative feature embeddings are learned for the accurate delineation of teeth boundaries. Extensive experiments on a clinical dental CBCT dataset demonstrate the superior performance of the proposed method compared with other state-of-the-art approaches.
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http://dx.doi.org/10.1109/TMI.2022.3179128DOI Listing
May 2022
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