Publications by authors named "Yue Zhao"

1,807 Publications

  • Page 1 of 1

Auricular acupressure for myopia prevention and control in children and its effect on choroid and retina: a randomized controlled trial protocol.

Trials 2021 Jun 7;22(1):387. Epub 2021 Jun 7.

Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.

Background: Nowadays, because of the increasing incidence, the prevention and control of myopia has become an urgent issue. In China, auricular acupressure has been commonly used in the clinical treatment of myopia in children, but the exact effectiveness remains unproven. The purpose of this trial is to observe the efficacy of auricular acupressure in myopia prevention and control, as well as its effect on the choroidal and retinal thickness.

Method/design: A total of 480 subjects at 8-9 years old will be randomized in a 1:1 ratio to an intervention group versus a control group. The intervention group will receive auricular acupressure for 12 months, while the control group will be taken as a blank control. The primary and secondary outcomes will be measured at baseline, and again at 3, 6, 9, and 12 months after recruitment. The myopia incidence (spherical equivalent ≤ - 0.50 D) and the mean change of spherical equivalent will be taken as the primary variables; the secondary outcome measures include axial length, uncorrected visual acuity, and choroidal and retinal thickness.

Discussion: This trial aims to evaluate the effectiveness of auricular acupressure for myopia prevention and control with objective evidence and to preliminarily explore the plausible mechanism and provide reference for adopting this approach to retard the onset and control the progression of myopia.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000038456 . Registered on September 23, 2020.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13063-021-05334-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186104PMC
June 2021

Novel Diabetic Foot Wound Dressing Based on Multifunctional Hydrogels with Extensive Temperature-Tolerant, Durable, Adhesive, and Intrinsic Antibacterial Properties.

ACS Appl Mater Interfaces 2021 Jun 7;13(23):26770-26781. Epub 2021 Jun 7.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Diabetic foot ulcers (DFUs) are hard-healing chronic wounds and susceptible to bacterial infection. Conventional hydrogel dressings easily lose water at high temperature or freeze at low temperature, making them unsuitable for long-term use or in extreme environments. Herein, a temperature-tolerant (-20 to 60 °C) antibacterial hydrogel dressing is fabricated by the assembly of polyacrylamide, gelatin, and ε-polylysine. Owing to the water/glycerin (Gly) binary solvent system, the resultant hydrogel (G-PAGL) displayed good heat resistance and antifreezing properties. Within the wide temperature range (-20 to 60 °C), all the desirable features of the hydrogel, including superstretchability (>1400%), enduring water retention, adhesiveness, and persistent antibacterial property, are quite stable. Remarkably, the hydrogel wound dressing displayed lasting and broad antibacterial activity against Gram-positive and Gram-negative bacteria. Satisfactorily, the double-network (DN) G-PAGL hydrogel dressing could effectively promote the healing of DFUs by accelerating collagen deposition, promoting angiogenesis, and inhibiting bacterial breed. As far as we know, this is the first time that the extensive temperature-tolerant DN hydrogel with antibacterial ability is developed to use as DFU wound dressing. The G-PAGL hydrogel provides more choices for DFU wound dressings that could be used in extreme environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c05514DOI Listing
June 2021

MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting .

Arch Anim Breed 2021 18;64(1):17-25. Epub 2021 Jan 18.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, China.

Adipocyte differentiation, which plays an important role in fat deposition, involves a complex molecular mechanism. MicroRNAs (miRNAs) are essential in this progress. Here, we showed that miR-25-3p expression had increased during goat intramuscular preadipocyte differentiation, which peaked at day 3. Using liposome transfection and qRT-PCR techniques, we found that knocking down miR-25-3p reduced the accumulation of lipid droplets by downregulating or upregulating the expression of , , , , and but upregulating the expression of . Overexpression of miR-25-3p results in the opposite. Furthermore, the dual luciferase assay showed that overexpression of miR-25-3p significantly inhibited luciferase activity of . These results showed that miR-25-3p has a binding site within the 3 -UTR of mRNA. Together, these findings indicate that miR-25-3p is a positive regulator of intramuscular preadipocyte differentiation via targeting to in goats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5194/aab-64-17-2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128059PMC
January 2021

Expression of Immune Checkpoint Regulators IDO, VISTA, LAG3, and TIM3 in Resected Pancreatic Ductal Adenocarcinoma.

Cancers (Basel) 2021 May 29;13(11). Epub 2021 May 29.

Department of General, Visceral, Cancer and Transplantation Surgery, University of Cologne, 50937 Cologne, Germany.

Pancreatic cancer features elaborate mechanisms of immune evasion. The potential of new immune molecules was explored to restore the antitumor immune response. If these immune molecules are associated with poor survival, specific drugs could take effect. Here, we analyze the expression of VISTA, LAG3, IDO, and TIM3 on tumor-infiltrating lymphocytes (TILs) and its impact on patient survival. We analyzed 153 pancreatic cancer patients from the prospectively managed database of the multicentered PANCALYZE study. Immunohistochemistry on a tissue microarray assessed VISTA, LAG3, IDO, and TIM3 expression of TILs from the patients undergoing primary resection. Complementarily, we analyzed publicly available transcriptomic data ( = 903). Successful completion of chemotherapy, and lymph node status were independent predictors of survival in the multivariate analysis of the clinicopathologic parameters. Fifteen tumors were exclusively VISTA-positive, thirteen tumors expressed VISTA together with TIM3, and ten tumors expressed VISTA together with IDO. Patients featuring tumors with high numbers of IDO-positive TILs had better patient survival ( = 0.037). VISTA, LAG3, and TIM3 expression did not correlate with survival. The analysis of publicly available data did not show survival differences. Tumors rarely co-express more than two immune molecules at the same time, and VISTA is most frequently co-expressed. Although IDO generally inhibits T-cell proliferation, a high expression of IDO was associated with improved survival. We expect immune checkpoint inhibitors against VISTA, LAG3, and TIM3 to be inefficient in a clinical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers13112689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198722PMC
May 2021

Aldo-Keto Reductase 1C3 Mediates Chemotherapy Resistance in Esophageal Adenocarcinoma via ROS Detoxification.

Cancers (Basel) 2021 May 16;13(10). Epub 2021 May 16.

Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, 50937 Cologne, Germany.

Esophageal adenocarcinoma (EAC) is one of the most lethal malignancies, and limits promising treatments. AKR1C3 represents a therapeutic target to combat the resistance in many cancers. However, the molecular mechanism of AKR1C3 in the chemotherapy resistance of EAC is still unclear. We found that the mRNA level of AKR1C3 was higher in EAC tumor tissues, and that high AKR1C3 expression might be associated with poor overall survival of EAC patients. AKR1C3 overexpression decreased cell death induced by chemotherapeutics, while knockdown of AKR1C3 attenuated the effect. Furthermore, we found AKR1C3 was inversely correlated with ROS production. Antioxidant NAC rescued chemotherapy-induced apoptosis in AKR1C3 knockdown cells, while the GSH biosynthesis inhibitor BSO reversed a protective effect of AKR1C3 against chemotherapy. AKT phosphorylation was regulated by AKR1C3 and might be responsible for eliminating over-produced ROS in EAC cells. Intracellular GSH levels were modulated by AKR1C3 and the inhibition of AKT could reduce GSH level in EAC cells. Here, we reported for the first time that AKR1C3 renders chemotherapy resistance through controlling ROS levels via AKT signaling in EAC cells. Targeting AKR1C3 may represent a novel strategy to sensitize EAC cells to conventional chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers13102403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156851PMC
May 2021

Proton Dynamics of Water Diffusion in Shrimp Hydrolysates Flour and Effects of Moisture Absorption on Its Properties.

Foods 2021 May 20;10(5). Epub 2021 May 20.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.

Moisture absorbed into shrimp hydrolysates (SHs) flour profoundly affected its properties. The unstored hydrolysate flour was called SHs-0h and SHs stored for 30 h at 25 °C and 75% relative humidity was named SHs-30. During the process of storage, the moisture dynamics in SHs flour were investigated by dynamic vapor sorption (DVS) and low-field nuclear magnetic resonance (LF-NMR). The effects of moisture absorption on the radicals scavenging rates of SHs flour were evaluated by electron paramagnetic resonance (EPR). The effects of moisture absorption on secondary structure were studied by mid-infrared (MIR) spectroscopy and infrared microimaging spectroscopy. The changes of volatile components were monitored by purge and trap coupled with gas chromatography-mass spectrometry (PT-GC-MS). DVS results showed that the moisture absorption rate of SHs flour could reach a maximum of 88.93%. Meanwhile, the water was transformed into more stable water with shorter relaxation times. The porous structure of the SHs-30 h flour disappeared and became smoother compared to SH-0 h flour. DPPH (31.09 ± 0.54%) and OH (26.62 ± 1.14%) radicals scavenging rates of SHs-30 h significantly reduced ( < 0.05) compared to that of SHs-0 h flour. The vibrations of the MIR absorbance peaks were changed. Finally, eight volatile components disappeared and six new volatile compounds were found. This study provided a theory basis for moisture dynamics in peptide flour during the storage process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods10051137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161016PMC
May 2021

Cytoplasmic RAD23B interacts with CORO1C to synergistically promote colorectal cancer progression and metastasis.

Cancer Lett 2021 May 29;516:13-27. Epub 2021 May 29.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

Colorectal cancers (CRCs) are characterized by diffuse infiltration of tumor cells into the regional lymph nodes and metastasis to distant organs, and its highly invasive nature contributes to disease recurrence and poor outcomes. The molecular mechanisms underlying CRC cell invasion remain incompletely understood. Here, we identified the upregulation of DNA damage repair-related protein RAD23B in CRC cells and tissues and showed that it associates with coronin 1C or coronin 3 (CORO1C) to facilitate invasion. We found that knockdown of RAD23B expression significantly inhibited the proliferation, invasion, and migration abilities of CRC cells both in vitro and in vivo, and suppressed the talin1/2/integrin/FAK/RhoA/Rac1/CORO1C signaling pathways. Interestingly, RAD23B interacted and co-localized with CORO1C, and CORO1C aggregated toward the margin of cancer cells in both CRC cells and tissues when RAD23B overexpressed. Mechanistically, overexpression of RAD23B and/or CORO1C further increased invadopodia formation and matrix degradation in SW480 and HCT8 CRC cells. Conversely, silencing of RAD23B expression suppressed tumorigenesis and liver metastasis in xenotransplant murine models. Furthermore, we found that RAD23B was significantly overexpressed in tumor tissues (n = 720) compared to adjacent non-tumor tissues (n = 694) of patients with CRC. Finally, we identified a strong correlation between higher levels of cytoplasmic expression of RAD23B, and poor prognosis and liver metastasis in CRC patients. Taken together, our data highlight a novel RAD23B-CORO1C signaling axis in CRC cell invasion and metastasis that may be of clinical significance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2021.05.033DOI Listing
May 2021

Structures of wild-type and H451N mutant human lymphocyte potassium channel K1.3.

Cell Discov 2021 Jun 1;7(1):39. Epub 2021 Jun 1.

Hefei National Laboratory of Physical Sciences at Microscale, Anhui Laboratory of Advanced Photonic Science and Technology, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41421-021-00269-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167131PMC
June 2021

Berberine Reverses Breast Cancer Multidrug Resistance Based on Fluorescence Pharmacokinetics and .

ACS Omega 2021 Apr 13;6(16):10645-10654. Epub 2021 Apr 13.

Department of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China.

Exploring the mechanism through which berberine (Ber) reverses the multidrug resistance (MDR) of breast cancer is of great importance. Herein, we used the methyl thiazolyl tetrazolium assay to determine the drug resistance and cytotoxicity of Ber and doxorubicin (DOX) alone or in combination on the breast cancer cell line MCF-7/DOX. The results showed that Ber could synergistically enhance the inhibitory effect of DOX on tumor cell proliferation , and the optimal combination ratio was Ber/DOX = 2:1. Using a luciferase reporter assay system combined with the bioluminescence imaging technology, the efflux kinetics of d-luciferin potassium salt in MCF-7/DOX cells treated with Ber was investigated. The results showed that Ber could significantly reduce the efflux of d-luciferin potassium salt in MCF-7/DOX cells. In addition, western blot and immunohistochemistry experiments showed that the expression of P-glycoprotein (P-gp/ABCB1) and multidrug resistance protein 1 (MRP1/ABCC1) in MCF-7/DOX cells was downregulated upon Ber treatment. Finally, high-performance liquid chromatography was used to investigate the effect of Ber on DOX tissue distribution , and the results showed that the uptake of DOX in tumor tissues increased significantly when combined with Ber ( < 0.05). Thus, the results illustrated that Ber can reverse MDR by inhibiting the efflux function of ATP-binding cassette transporters and downregulating their expression levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c06288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153757PMC
April 2021

Oroxylin A maintains the colonic mucus barrier to reduce disease susceptibility by reconstituting a dietary fiber-deprived gut microbiota.

Cancer Lett 2021 Sep 28;515:73-85. Epub 2021 May 28.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, People's Republic of China. Electronic address:

Dietary fiber intake helps to maintain gut homeostasis. Fiber deficiency causes commensals to utilize mucins as an energy source to destroy mucus layer, thus promoting susceptibility to inflammatory bowel disease. Here, we reported that oroxylin A, a natural flavonoid, ameliorated low-grade colonic inflammation caused by fiber deficiency, alleviated colitis, and further prevented colitis-associated colon cancer in mice. The anti-inflammatory effect of oroxylin A was due to its alteration of gut microbiota. We found that the levels of Eubacterium coprostanoligenes was significantly increased by oroxylin A and the colonized Eubacterium coprostanoligenes significantly protected against colitis and carcinogenesis in colon of mice. Together, our results in this study suggest that oroxylin A may reduce the susceptibility to intestinal diseases by increasing the level of Eubacterium coprostanoligenes which could provide a therapeutic alternation for the treatment of intestinal diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2021.05.018DOI Listing
September 2021

Geranylgeranyl pyrophosphate-mediated protein geranylgeranylation regulates endothelial cell proliferation and apoptosis during vasculogenesis in mouse embryo.

J Genet Genomics 2021 Apr 27. Epub 2021 Apr 27.

Ministry of Education Key Laboratory of Model Animals for Disease Study, Model Animal Research Center and School of Medicine, Nanjing University, National Resource Center for Mutant Mice, Nanjing 210093, China. Electronic address:

Vascular development is essential for the establishment of the circulatory system during embryonic development and requires the proliferation of endothelial cells. However, the underpinning regulatory mechanisms are not well understood. Here, we report that geranylgeranyl pyrophosphate (GGPP), a metabolite involved in protein geranylgeranylation, plays an indispensable role in embryonic vascular development. GGPP is synthesized by geranylgeranyl pyrophosphate synthase (GGPPS) in the mevalonate pathway. The selective knockout of Ggpps in endothelial cells led to aberrant vascular development and embryonic lethality, resulting from the decreased proliferation and enhanced apoptosis of endothelial cells during vasculogenesis. The defect in protein geranylgeranylation induced by GGPP depletion inhibited the membrane localization of RhoA and enhanced yes-associated protein (YAP) phosphorylation, thereby prohibiting the entry of YAP into the nucleus and the expression of YAP target genes related to cell proliferation and the antiapoptosis process. Moreover, inhibition of the mevalonate pathway by simvastatin induced endothelial cell proliferation defects and apoptosis, which were ameliorated by GGPP. Geranylgeraniol (GGOH), a precursor of GGPP, ameliorated the harmful effects of simvastatin on vascular development of developing fetuses in pregnant mice. These results indicate that GGPP-mediated protein geranylgeranylation is essential for endothelial cell proliferation and the antiapoptosis process during embryonic vascular development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2021.03.009DOI Listing
April 2021

Soyasaponin A Alleviates Steatohepatitis Possibly through Regulating Bile Acids and Gut Microbiota in the Methionine and Choline-Deficient (MCD) Diet-induced Nonalcoholic Steatohepatitis (NASH) Mice.

Mol Nutr Food Res 2021 May 28:e2100067. Epub 2021 May 28.

Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.

Scope: Nonalcoholic steatohepatitis (NASH) is a chronic progressive disease with complex pathogenesis of which the bile acids (BAs) and gut microbiota are involved. Soyasaponins (SS) exhibits many health-promoting effects including hepatoprotection, but its prevention against NASH is unclear. This study aims to investigate the preventive bioactivities of SS monomer (SS-A ) against NASH and further clarify its mechanism by targeting the BAs and gut microbiota.

Methods And Results: The methionine and choline deficient (MCD) diet-fed male C57BL/6 mice were intervened with obeticholic acid or SS-A for 16 weeks. Hepatic pathology is assessed by hematoxylin-eosin and Masson's trichrome staining. BAs in serum, liver, and colon are measured by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-TQMS). Gut microbiota in caecum are determined by 16S rDNA amplicon sequencing. In the MCD diet-induced NASH mice, SS-A significantly reduces hepatic steatosis, lobular inflammation, ballooning, nonalcoholic fatty liver disease activity score (NAS) scores, and fibrosis, decreases Erysipelotrichaceae (Faecalibaculum) and Lactobacillaceae (Lactobacillus) and increases Desulfovibrionaceae (Desulfovibrio). Moreover, SS-A reduces serum BAs accumulation and promotes fecal BAs excretion. SS-A changes the BAs profiles in both liver and serum and specifically increases the taurohyodeoxycholic acid (THDCA) level. Faecalibaculum is negatively correlated with serum THDCA.

Conclusion: SS-A alleviates steatohepatitis possibly through regulating BAs and gut microbiota in the MCD diet-induced NASH mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mnfr.202100067DOI Listing
May 2021

Erbium Single Atom Composite Photocatalysts for Reduction of CO under Visible Light: CO Molecular Activation and 4f Levels as an Electron Transport Bridge.

Small 2021 May 27:e2102089. Epub 2021 May 27.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin, 150080, China.

It is still challenging to design a stable and efficient catalyst for visible-light CO reduction. Here, Er single atom composite photocatalysts are successfully constructed based on both the special role of Er and the special advantages of Zn GeO /g-C N heterojunction in the photocatalysis reduction of CO . Especially, Zn GeO :Er /g-C N obtained by in situ synthesis is not only more conducive to the tight junction of Zn GeO and g-C N , but also more favorable for g-C N to anchor rare-earth atoms. Under visible-light irradiation, Zn GeO :Er /g-C N shows more than five times enhancement in the catalytic efficiency compared to that of pure g-C N without any sacrificial agent in the photocatalytic reaction system. A series of theoretical and experimental results show that the charge density around Er, Ge, Zn, and O increases compared with Zn GeO :Er , while the charge density around C decreases compared with g-C N . These results show that an efficient way of electron transfer is provided to promote charge separation, and the dual functions of CO molecular activation of Er single atom and 4f levels as electron transport bridge are fully exploited. The pattern of combining single-atom catalysis and heterojunction opens up new methods for enhancing photocatalytic activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102089DOI Listing
May 2021

Comparative molecular dynamics simulations identify a salt-sensitive loop responsible for the halotolerant activity of GH5 cellulases.

J Biomol Struct Dyn 2021 May 27:1-11. Epub 2021 May 27.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Halotolerant glycoside hydrolases (GH) have broad application potentials in biorefinery industries. Elucidating the structure-activity relationship underlying the halotolerant catalysis is essential to design superior biocatalysts. Here, we performed molecular dynamics simulations to investigate the structural dynamics of two GH5 cellulases, namely the halotolerant Cel5R and non-halotolerant Cel5A. Through characterizing the physical properties at different salt concentrations, the results revealed that the overall structures of Cel5R and Cel5A were marginally affected by the increase in salt concentrations. However, a salt-sensitive loop was identified from both Cel5R and Cel5A based on its significantly increased flexibility at high salt concentrations. Importantly, compared to Cel5A the salt-sensitive loop of Cel5R engaged more sodium ions and water molecules around the active site of the enzyme. Besides, the unique residue motif of the salt-sensitive loop in Cel5R formed more intramolecular hydrogen bonds, stabilizing the active architecture of Cel5R at high salt concentrations. Collectively, the structural and dynamic differences may contribute to the various catalytic halotolerance of Cel5R and Cel5A. These findings provide mechanistic insight into the halotolerant catalysis and will guide the ration design of GH5 cellulases with improved catalytic properties.Communicated by Ramaswamy H. Samy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07391102.2021.1930167DOI Listing
May 2021

Brain functional network modeling and analysis based on fMRI: a systematic review.

Cogn Neurodyn 2021 Jun 31;15(3):389-403. Epub 2020 Aug 31.

College of Engineering, The University of Texas at El Paso, El Paso, TX USA.

In recent years, the number of patients with neurodegenerative diseases (i.e., Alzheimer's disease, Parkinson's disease, mild cognitive impairment) and mental disorders (i.e., depression, anxiety and schizophrenia) have increased dramatically. Researchers have found that complex network analysis can reveal the topology of brain functional networks, such as small-world, scale-free, etc. In the study of brain diseases, it has been found that these topologies have undergoed abnormal changes in different degrees. Therefore, the research of brain functional networks can not only provide a new perspective for understanding the pathological mechanism of neurological and psychiatric diseases, but also provide assistance for the early diagnosis. Focusing on the study of human brain functional networks, this paper reviews the research results in recent years. First, this paper introduces the background of the study of brain functional networks under complex network theory and the important role of topological properties in the study of brain diseases. Second, the paper describes how to construct a brain functional network using neural image data. Third, the common methods of functional network analysis, including network structure analysis and disease classification, are introduced. Fourth, the role of brain functional networks in pathological study, analysis and diagnosis of brain functional diseases is studied. Finally, the paper summarizes the existing studies of brain functional networks and points out the problems and future research directions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11571-020-09630-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131458PMC
June 2021

High Proton-Conductivity in Covalently Linked Polyoxometalate-Organoboronic Acid-Polymers.

Angew Chem Int Ed Engl 2021 May 26. Epub 2021 May 26.

Henan Normal University School of Chemistry and Chemical Engineering, Chemistry, CHINA.

The controlled bottom-up design of polymers with metal oxide backbones is a grand challenge in materials design, as it could give unique control over the resulting chemical properties. Here, we report a 1D-organo-functionalized polyoxometalate polymer featuring a purely inorganic backbone. The polymer is self-assembled from two types of monomers, inorganic Wells-Dawson-type polyoxometalates and aromatic organo-boronates. Their covalent linkage results in 1D polymer strands, which combine an inorganic oxide backbone (based on B-O and Nb-O linkages) with functional organic side-chains. The polymer shows high bulk proton conductivity of up to 1.59 ⨯ 10-1 S cm-1 at 90 °C and 98 % relative humidity. This synthetic approach could lead to a new class of organic-inorganic polymers where function can be designed by controlled tuning of the monomer units.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202104886DOI Listing
May 2021

Protective and therapeutic experience of perioperative safety in extremely elderly patients with biliary diseases.

Medicine (Baltimore) 2021 May;100(21):e26159

Department of General Surgery, Beijing Electric Power Hospital, State Grid Corporation of China, Capital Medical University, Beijing, PR China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154467PMC
May 2021

Heavy metal pollution in urban river sediment of different urban functional areas and its influence on microbial community structure.

Sci Total Environ 2021 Jul 10;778:146383. Epub 2021 Mar 10.

School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, PR China.

In this study, the Songgang River (SR) was selected as a typical contaminated urban river in a highly urbanized city (Shenzhen) that is extensively polluted by heavy metals (HMs). Five representative sampling sites were selected from different urban functional areas along the SR, and the spatial and vertical distributions of HMs and the related environmental risk were investigated. In addition, the distribution variability, composition, and abundance of microbial communities, as well as the correlation between the abundance of the operational taxonomic units (OTUs) and the HM contents were analyzed. The spatial distribution of HMs in the sediment revealed wide variation among the different urban functional areas. Industrial and residential areas had higher HM contents, following the order of Cu > Zn > Ni > Cr > Pb. In addition, the vertical characterization (5-300 cm) of HM content showed a decreasing trend with depth, with a distinct layer around 120-180 cm that might have been caused by anthropogenic activity. An ecological risk assessment indicated that Cu, Ni, and Cr pose high potential risks in these industrial and residential areas (at the depth of 5-180 cm). Furthermore, microbial community analysis indicated that some HM-tolerant bacteria (e.g., Gallionella, Acidovorax, Arenimonas, Curvibacter, and Sideroxydans) were dominant in the 5-120 cm layer, corresponding to high HM contents. A canonical correspondence analysis and co-occurrence network further confirmed that there was a strong correlation among the urban functional areas, HM contents, and the abundance of microorganisms in the urban river sediment. The results of this study have the potential to provide a bio-augmentation strategy for the in-situ bioremediation of sediment contaminated by HMs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.146383DOI Listing
July 2021

Comparison of two proposed changes to the current nodal classification for non-small cell lung cancer based on the number and ratio of metastatic lymph nodes.

Chest 2021 May 21. Epub 2021 May 21.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. Electronic address:

Background: The current nodal classification is unsatisfactory in distinguishing the prognostically heterogeneous N1 or N2 non-small cell lung cancer (NSCLC).

Research Question: Is the combination of the current N category and the number of metastatic lymph nodes (N#number) or the combination of the current N category and the ratio of the number of positive to resected lymph nodes (N#ratio) better than the current N category alone?

Study Design And Methods: We identified 2162 patients with N1 or N2 NSCLC from the Surveillance, Epidemiology, and End Results database (2004 to 2016). We classified these patients into three N#number categories (N#number-1, N#number-2a, N#number-2b) and three N#ratio categories (N#ratio-1, N#ratio-2a, N#ratio-2b). Lung cancer-specific survival (LCSS) were compared using the Kaplan-Meier method. The prognostic significance of the new nodal classifications was validated across each tumor size category (≦3 cm, 3-5 cm, 5-7cm, >7 cm). Cox proportional hazards regression was used to evaluate the association between each nodal classification and LCSS.

Results: The survival curves showed clear differences between each pair of N#number and N#ratio categories. A significant tendency towards the deterioration of LCSS from N#number-1 to N#number-2b was observed in all tumor size categories. However, the differences between each pair of N#ratio categories were significant only in tumors from 3 to 7 cm. Although all three nodal classifications were independent prognostic indicators, the N#number classification provided more accurate prognostic stratifications compared with the N#ratio classification and the current nodal classification.

Interpretation: The N#number classification followed by the N#ratio classification might be better prognostic determinants than the current nodal classification in prognostically heterogeneous N1 or N2 NSCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chest.2021.05.014DOI Listing
May 2021

Role of lung and gut microbiota on lung cancer pathogenesis.

J Cancer Res Clin Oncol 2021 May 20. Epub 2021 May 20.

Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, 266000, China.

Background: Lung cancer is the leading cause of cancer-related deaths worldwide (Ferlay et al., Int J Cancer 136:E359-386, 2015). In addition, lung cancer is associated with the highest mortality among all cancer types (Wu et al., Exp Ther Med 16:3004-3010, 2018). Previous studies report that microbiota play an important role in lung cancer. Notably, changes in lung and gut microbiota, are associated with progression of lung cancer. Several studies report that lung and gut microbiome promote lung cancer initiation and development by modulating metabolic pathways, inhibiting the function of immune cells, and producing pro-inflammatory factors. In addition, some factors such as microbiota dysbiosis, affect production of bacteriotoxins, genotoxicity and virulence effect, therefore, they play a key role in cancer progression. These findings imply that lung and gut microbiome are potential markers and targets for lung cancer. However, the role of microbiota in development and progression of lung cancer has not been fully explored.

Purpose: The aim of this study was to systemically review recent research findings on relationship of lung and gut microbiota with lung cancer. In addition, we explored gut-lung axis and potential mechanisms of lung and gut microbiota in modulating lung cancer progression.

Conclusion: Pulmonary and intestinal flora influence the occurrence, development, treatment and prognosis of lung cancer, and will provide novel strategies for prevention, diagnosis, and treatment of lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00432-021-03644-0DOI Listing
May 2021

A comparison of the growth status, level of blood glucose, and lipid metabolism in small for gestational age and appropriate for gestational age girls with central precocious puberty: a retrospective study.

Transl Pediatr 2021 Apr;10(4):783-789

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: To compare the physical development status, level of blood glucose and lipid metabolism in small for gestational age (SGA) and appropriate for gestational age (AGA) groups with central precocious puberty (CPP).

Methods: This was a retrospective study. Three hundred and twenty-two girls with CPP were divided into the AGA group (304 cases) and the SGA group (18 cases). Physical index such as height, weight, and body mass index (BMI), as well as sex hormones, adrenal androgens, blood lipid levels, fasting blood glucose, insulin levels, and homeostasis model assessment of insulin resistance (HOMA-IR) were compared between the two groups.

Results: Height, weight, and BMI in the SGA group were lower than those in the AGA group (P<0.05). The level of LH/FSH, estradiol, testosterone, DHEA and androstenedione had no significant difference between the SGA group and AGA group (P>0.05). The fasting blood glucose, insulin level, HOMA-IR, high-density lipoprotein (HDL) and the average level of triglycerides were similar between these two groups (P>0.05). There was a statistically significant difference in total cholesterol and low-density lipoprotein (LDL) between the two groups (P<0.05). However, the blood lipids and blood glucose in both groups were within the normal range.

Conclusions: The height, weight, BMI, serum cholesterol and LDL of girls in SGA with CPP were significantly lower than that of those girls born AGA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tp-20-366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107858PMC
April 2021

Computational Bioactivity Fingerprint Similarities To Navigate the Discovery of Novel Scaffolds.

J Med Chem 2021 06 19;64(11):7544-7554. Epub 2021 May 19.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410003, China.

As one of the central tasks of modern medicinal chemistry, scaffold hopping is expected to lead to the discovery of structural novel biological active compounds and broaden the chemical space of known active compounds. Here, we report the computational bioactivity fingerprint (CBFP) for easier scaffold hopping, where the predicted activities in multiple quantitative structure-activity relationship models are integrated to characterize the biological space of a molecule. In retrospective benchmarks, the CBFP representation shows outstanding scaffold hopping potential relative to other chemical descriptors. In the prospective validation for the discovery of novel inhibitors of poly [ADP-ribose] polymerase 1, 35 predicted compounds with diverse structures are tested, 25 of which show detectable growth-inhibitory activity; beyond this, the most potent (compound 6) has an IC of 0.263 nM. These results support the use of CBFP representation as the bioactivity proxy of molecules to explore uncharted chemical space and discover novel compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jmedchem.1c00234DOI Listing
June 2021

Selection of metastasis competent subclones in the tumour interior.

Nat Ecol Evol 2021 May 17. Epub 2021 May 17.

Cancer Research UK Lung Cancer Centre of Excellence, University College London Cancer Institute, London, UK.

The genetic evolutionary features of solid tumour growth are becoming increasingly well described, but the spatial and physical nature of subclonal growth remains unclear. Here, we utilize 102 macroscopic whole-tumour images from clear cell renal cell carcinoma patients, with matched genetic and phenotypic data from 756 biopsies. Utilizing a digital image processing pipeline, a renal pathologist marked the boundaries between tumour and normal tissue and extracted positions of boundary line and biopsy regions to X and Y coordinates. We then integrated coordinates with genomic data to map exact spatial subclone locations, revealing how genetically distinct subclones grow and evolve spatially. We observed a phenotype of advanced and more aggressive subclonal growth in the tumour centre, characterized by an elevated burden of somatic copy number alterations and higher necrosis, proliferation rate and Fuhrman grade. Moreover, we found that metastasizing subclones preferentially originate from the tumour centre. Collectively, these observations suggest a model of accelerated evolution in the tumour interior, with harsh hypoxic environmental conditions leading to a greater opportunity for driver somatic copy number alterations to arise and expand due to selective advantage. Tumour subclone growth is predominantly spatially contiguous in nature. We found only two cases of subclone dispersal, one of which was associated with metastasis. The largest subclones spatially were dominated by driver somatic copy number alterations, suggesting that a large selective advantage can be conferred to subclones upon acquisition of these alterations. In conclusion, spatial dynamics is strongly associated with genomic alterations and plays an important role in tumour evolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41559-021-01456-6DOI Listing
May 2021

The Complexity of Blood Pressure Fluctuation Mediated the Effects of Hypertension on Walking Speed in Older Adults.

Front Aging Neurosci 2021 29;13:640942. Epub 2021 Apr 29.

Hinda and Arthur Marcus Institute for Aging Research, Hebrew SeniorLife, Roslindale, MA, United States.

Older adults with hypertension often had diminished walking performance. The underlying mechanism through which hypertension affects walking performance, however, has not been fully understood. We here measured the complexity of the continuous systolic (SBP) and diastolic (DBP) blood pressure fluctuation, grade of white matter lesions (WMLs), and cognitive function and used structural equation modeling (SEM) to examine the interrelationships between hypertension, BP complexity, WMLs, cognitive function, and walking speed in single- and dual-task conditions. A total of 152 older adults with age > 60 years (90 hypertensive and 62 normotensive participants) completed one MRI scan of brain structure, a finger BP assessment of at least 10 min, Mini-Mental State Examination (MMSE) to assess cognitive function, and 10-meter walking tests in single (i.e., normal walking) and dual tasks (i.e., walking while performing a serial subtraction of three from a random three-digit number). The grade of WMLs was assessed using the total score of Fazekas scale; the complexity of SBP and DBP was measured using multiscale entropy (MSE), and the walking performance was assessed by walking speed in single- and dual-task conditions. As compared to normotensives, hypertensive older adults had significantly slower walking speed, lower complexity of SBP and DBP, greater grade of WMLs, and poorer cognitive function ( < 0.03). Those with lower BP complexity (β > 0.31, < 0.003), greater WML grade (β < -0.39, < 0.0002), and/or poorer cognitive function (β < -0.39, < 0.0001) had slower walking speed in single- and/or dual-task conditions. The SEM model demonstrated significant total effects of hypertension on walking speed, and such effects were mediated by BP complexity only, or BP complexity, WML grade, and cognitive function together. This study demonstrates the cross-sectional association between the complexity of continuous beat-to-beat BP fluctuation, WML grade, cognitive function, and walking speed in hypertensive and normotensive older adults, revealing a potential mechanism that hypertension may affect walking performance in older adults through diminished BP complexity, increased WML grade, and decreased cognitive function, and BP complexity is an important factor for such effects. Future longitudinal studies are warranted to confirm the findings in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2021.640942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118160PMC
April 2021

Biclonal evolution of chronic lymphocytic leukaemia.

Br J Haematol 2021 May 16. Epub 2021 May 16.

Department of Pathology, Duke University School of Medicine, Durham, NC, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bjh.17543DOI Listing
May 2021

Continuous insulation strategy of organic waste composting in cold region: Based on cold-adapted consortium.

Bioresour Technol 2021 Sep 7;335:125257. Epub 2021 May 7.

College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

Compost is a self-heating process for organic waste. Microbes are the main executors in composting process. However, due to low temperature in winter and low efficiency of composting, a lot of heat is lost in composting. In this study, taking-out and feed-batch composting method (TFC) can supplement nutrition and improve composting microenvironment. Compared with NC, the amount of carbohydrates, protein and fat decomposed by TFC increase by 56.8%, 237% and 122%, respectively, in the composting start-up period (0-100 h). Structure and function of microbial community have changed due to stimulation of cold-adapted consortium. In addition, this study shows that core bacteria stimulate cooperation among different bacteria in the organic components metabolism networks. Finally, based on the important role of cold-adapted consortium, the sustainable heating strategy of composting system is put forward, which converts organic wastes into released heat for daily heating and hot water preparation, leading role of cold-adapted consortium.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.125257DOI Listing
September 2021

Multinuclear transition metal-containing polyoxometalates constructed from Nb/W mixed-addendum precursors: synthesis, structures and catalytic performance.

Dalton Trans 2021 May 14. Epub 2021 May 14.

School of Chemistry and Chemical Engineering, Henan Key Laboratory of Boron Chemistry and Advanced Energy Materials, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Centre of Henan Province for Green Manufacturing of Fine Chemicals, Henan Normal University, Xinxiang, 453007, China. and College of Chemistry and Molecular Engineering Zhengzhou University, Zhengzhou, 450001, China.

Four new transition metal-containing Nb/W mixed-addendum POM trimers with the formula H19[M4(H2O)x(P2W15Nb3O62)3]·m(HCOOH)·nH2O (M = Cu, x = 15, m = 0, and n = 21, Cu-POM; M = Co, x = 7, m = 0, and n = 15, Co-POM; M = Mn, x = 7, m = 6, and n = 18, Mn-POM; and M = Zn, x = 7, m = 0, and n = 23, Zn-POM) have been synthesized by a solvothermal method in a water-ethanol mixed solvent. All the four compounds were characterized by single-crystal X-ray diffraction, powder X-ray diffraction (XRD), IR spectroscopy, thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy (XPS). These compounds can serve as efficient heterogeneous catalysts for the cyanosilylation of different carbonyl compounds under ambient temperature and solvent-free conditions, and Cu-POM shows much better catalytic performance than the other three compounds. The cycle experiment showed that Cu-POM can be reused for at least five cycles without significant loss of catalytic activity. The IR spectroscopy and XRD analysis revealed that Cu-POM can retain its integrity after catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1dt00924aDOI Listing
May 2021

Nanoplastic-Induced Genotoxicity and Intestinal Damage in Freshwater Benthic Clams (): Comparison with Microplastics.

ACS Nano 2021 May 14. Epub 2021 May 14.

The Key Laboratory of Water and Sediment Sciences, Ministry of Education, School of Environment, Beijing Normal University, Beijing 100875, P.R. China.

With the wide application of plastics in daily life, nanoplastics (NPs) are ubiquitous in freshwater environments. However, to date, few studies have focused on the mechanism underlying the toxicity of NPs, and the differences between this mechanism and that governing the toxicity of MPs have also not been thoroughly characterized. In this study, the genotoxicity, intestinal damage, and intestinal flora in exposed to micro/nanoplastics were investigated through RNA sequencing, histopathology, and 16S rRNA sequencing, respectively. Significant differences in differentially expressed genes (DEGs) were observed between MP and NP exposure groups. It was observed that NPs preferentially elicited the process related to cellular components and triggered the apoptosis through the mitochondrial pathway in various tissues, especially in indirectly contacted tissues, while MPs induced the innate immune response and activated the complement and coagulation cascades (complement system) pathway. Both MPs and NPs can induce an inflammatory response and cause epithelial damage in the intestines, and they can notably change the gut microbial community structure. However, the abundance of pathogenic bacteria (., ) was observed to increase only in the MP-treated group, which exacerbated intestinal damage. Unlike MPs, the effect of NPs on the intestinal microflora was highly limited, while NPs elicited more severe damage to the intestinal mucosal barrier. The results of this study may help to elucidate the toxicity mechanisms governing the responses of bivalves to MPs and NPs and to evaluate the detriment of MPs and NPs to the benthic ecosystem.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c02407DOI Listing
May 2021

Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen-2.

Exp Ther Med 2021 Jul 22;22(1):667. Epub 2021 Apr 22.

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.

Ginsenosides are important active components in . In the present study, total ginsenosides (TGNs) were demonstrated to enhance autophagy by promoting acidic vacuole organelle formation, recruitment of enhanced green fluorescent protein-microtubule-associated protein light chain 3 and expression of autophagy-related factors in cervical cancer cell lines. TGN markedly increased the expression of p62 at the transcriptional level, but decreased p62 protein expression in the presence of actinomycin D. The autophagic regulatory effect was reversible. TGN (≤120 µg/ml) did not affect the proliferation of cervical cancer cells under normal culture conditions, but markedly inhibited the growth of serum-deprived cells. Treatment with an inhibitor of autophagy (3-methyladenine) impaired TGN-induced cell death. This suggested that TGN caused autophagic cell death. In addition, western blot analysis demonstrated that the protein level of bone marrow stromal antigen-2 (BST-2) was downregulated by TGN. Upregulation of BST-2 reduced cell death. The results of the combined actions of various monomeric ginsenosides in TGN provide the molecular basis to develop TGN as a promising candidate for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112150PMC
July 2021

Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis.

J Anim Sci Biotechnol 2021 May 10;12(1):63. Epub 2021 May 10.

Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Background: Gram-negative bacterial infections have a major economic impact on both the livestock industry and public health. Toll-like receptor 4 (TLR4) plays a crucial role in host defence against Gram-negative bacteria. Exploring the defence mechanism regulated by TLR4 may provide new targets for treatment of inflammation and control of bacterial infections. In a previous study, we generated transgenic sheep overexpressing TLR4 by microinjection to improve disease resistance. The defence mechanism through which TLR4 overexpression protected these sheep against pathogens is still not fully understood.

Results: In the present study, we used Escherichia coli to infect monocytes isolated from peripheral blood of the animal model. The overexpression of TLR4 strongly enhanced the percentage of endocytosis and capacity of elimination in monocytes during the early stages of infection. This phenomenon was mainly due to overexpression of TLR4 promoting caveolae-mediated endocytosis. Pretreatment of the transgenic sheep monocytes with inhibitors of TLR4, Src signalling, or the caveolae-mediated endocytosis pathway reduced the internalization of bacteria, weakened the ability of the monocytes to eliminate the bacteria, and increased the pH of the endosomes.

Conclusion: Together, our results reveal the effects of TLR4 on the control of E. coli infection in the innate immunity of sheep and provide crucial evidence of the caveolae-mediated endocytosis pathway required for host resistance to invading bacteria in a large animal model, providing theoretical support for breeding disease resistance in the future. Furthermore, Src and caveolin 1 (CAV1) could be potentially valuable targets for the control of infectious diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40104-021-00585-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108469PMC
May 2021