Publications by authors named "Yue Yin"

379 Publications

The contribution of metamemory beliefs to the font size effect on judgments of learning: Is word frequency a moderating factor?

PLoS One 2021 20;16(9):e0257547. Epub 2021 Sep 20.

Collaborative Innovation Center of Assessment for Basic Education Quality, Beijing Normal University, Beijing, China.

Previous studies found that metamemory beliefs dominate the font size effect on judgments of learning (JOLs). However, few studies have investigated whether beliefs about font size contribute to the font size effect in circumstances of multiple cues. The current study aims to fill this gap. Experiment 1 adopted a 2 (font size: 70 pt vs. 9 pt) * 2 (word frequency (WF): high vs. low) within-subjects design. The results showed that beliefs about font size did not mediate the font size effect on JOLs when multiple cues (font size and WF) were simultaneously provided. Experiment 2 further explored whether WF moderates the contribution of beliefs about font size to the font size effect, in which a 2 (font size: 70 pt vs. 9 pt, as a within-subjects factor) * 2 (WF: high vs. low, as a between-subjects factor) mixed design was used. The results showed that the contribution of beliefs about font size to the font size effect was present in a pure list of low-frequency words, but absent in a pure list of high-frequency words. Lastly, a meta-analysis showed evidence supporting the proposal that the contribution of beliefs about font size to the font size effect on JOLs is moderated by WF. Even though numerous studies suggested beliefs about font size play a dominant role in the font size effect on JOLs, the current study provides new evidence suggesting that such contribution is conditional. Theoretical implications are discussed.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257547PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452059PMC
September 2021

Hybrid Li-Ion Capacitor Operated within an All-Climate Temperature Range from -60 to +55 °C.

ACS Appl Mater Interfaces 2021 Sep 20. Epub 2021 Sep 20.

Department of Chemistry, Fudan University, Shanghai 200433, China.

Lithium-ion capacitors (LICs) have been considered as an advanced energy storage system owing to their high energy and power densities. However, their application in a wide temperature range is still a great challenge due to the reduced ionic conductivity of the electrolyte and the poor electric conductivity of the battery-type transition metal oxide electrodes. Herein, an all-climate LIC is well-fabricated with [email protected] graphite as the anode and activated carbon as the cathode in an optimized electrolyte, which can be operated within a wide temperature range from -60 to +55 °C. Benefitting from the synergetic effect of the improved electrode and electrolyte, the LIC exhibits an outstanding energy density of 119 W h kg and a power density of 5110 W kg based on the total mass of both negative and positive electrodes. Moreover, it can deliver a capacity retention of as high as 42% at -60 °C and function at a superior rate capability at a high temperature of +55 °C, which exhibits an all-climate feature and the potential for wide applications under some extreme conditions.
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http://dx.doi.org/10.1021/acsami.1c14308DOI Listing
September 2021

Combining multisurface model and Gouy-Chapman-Stern model to predict cadmium uptake by cabbage (Brassica Chinensis L.) in soils.

J Hazard Mater 2021 08 29;416:126260. Epub 2021 May 29.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, The Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Cadmium is an extremely toxic substance known to cause serious health problems. The uptake of Cd in plants is critically affected by dissolved Cd in soil porewater, controlled by soil physicochemical properties. Rhizo-availability of Cd is assumed, as the Cd fraction is found on the plasma membrane of surface root cells. Based on the theory of Cd transformation in soil-crop systems, we established a novel combined mechanistic model related to soil, soil solutions, and crops. The combined model comprises a multisurface model (MSMs; solid adsorbent and porewater) and the Gouy-Chapman-Stern model (GCS; porewater and root surface). The results suggested that in mildly contaminated soil samples, optimum prediction was achieved when DTPA-extractable Cd was used as input variable (R = 0.723). Our approach was superior to single-step model calculation (MSMs: R = 0.613; GCS: R = 0.629) and prediction based on extractable soil Cd (R = 0.281). Introducing DTPA extraction expanded the range of model applications at different soil pHs. Our proposed mechanism model was based on soil physicochemical properties for Cd migration from soil to cabbage. Our model showed promise in predicting Cd bioavailability in soil with a wide pH range and evaluating soil risk near the standard Cd safety level.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126260DOI Listing
August 2021

Effect of Body Mass Index on the Prognosis of Liver Cirrhosis.

Front Nutr 2021 20;8:700132. Epub 2021 Aug 20.

Liver Cirrhosis Study Group, Department of Gastroenterology, General Hospital of Northern Theater Command (formerly called General Hospital of Shenyang Military Area), Shenyang, China.

At present, the association of body mass index (BMI) with the prognosis of liver cirrhosis is controversial. Our retrospective study aimed to evaluate the impact of BMI on the outcome of liver cirrhosis. In the first part, long-term death was evaluated in 436 patients with cirrhosis and without malignancy from our prospectively established single-center database. In the second part, in-hospital death was evaluated in 379 patients with cirrhosis and with acute gastrointestinal bleeding (AGIB) from our retrospective multicenter study. BMI was calculated and categorized as underweight (BMI <18.5 kg/m), normal weight (18.5 ≤ BMI < 23.0 kg/m), and overweight/obese (BMI ≥ 23.0 kg/m). In the first part, Kaplan-Meier curve analyses demonstrated a significantly higher cumulative survival rate in the overweight/obese group than the normal weight group ( = 0.047). Cox regression analyses demonstrated that overweight/obesity was significantly associated with decreased long-term mortality compared with the normal weight group [hazard ratio (HR) = 0.635; 95% CI: 0.405-0.998; = 0.049] but not an independent predictor after adjusting for age, gender, and Child-Pugh score (HR = 0.758; 95%CI: 0.479-1.199; = 0.236). In the second part, Kaplan-Meier curve analyses demonstrated no significant difference in the cumulative survival rate between the overweight/obese and the normal weight groups ( = 0.094). Cox regression analyses also demonstrated that overweight/obesity was not significantly associated with in-hospital mortality compared with normal weight group (HR = 0.349; 95%CI: 0.096-1.269; = 0.110). In both of the two parts, the Kaplan-Meier curve analyses demonstrated no significant difference in the cumulative survival rate between underweight and normal weight groups. Overweight/obesity is modestly associated with long-term survival in patients with cirrhosis but not an independent prognostic predictor. There is little effect of overweight/obesity on the short-term survival of patients with cirrhosis and with AGIB.
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http://dx.doi.org/10.3389/fnut.2021.700132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417598PMC
August 2021

Separable Microneedle Patch to Protect and Deliver DNA Nanovaccines Against COVID-19.

ACS Nano 2021 Sep 2. Epub 2021 Sep 2.

CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China.

The successful control of coronavirus disease 2019 (COVID-19) pandemic is not only relying on the development of vaccines, but also depending on the storage, transportation, and administration of vaccines. Ideally, nucleic acid vaccine should be directly delivered to proper immune cells or tissue (such as lymph nodes). However, current developed vaccines are normally treated through intramuscular injection, where immune cells do not normally reside. Meanwhile, current nucleic acid vaccines must be stored in a frozen state that may hinder their application in developing countries. Here, we report a separable microneedle (SMN) patch to deliver polymer encapsulated spike (or nucleocapsid) protein encoding DNA vaccines and immune adjuvant for efficient immunization. Compared with intramuscular injection, SMN patch can deliver nanovaccines into intradermal for inducing potent and durable adaptive immunity. IFN-γCD4/8 and IL-2CD4/8 T cells or virus specific IgG are significantly increased after vaccination. Moreover, results show the SMN patches can be stored at room temperature for at least 30 days without decreases in immune responses. These features of nanovaccines-laden SMN patch are important for developing advanced COVID-19 vaccines with global accessibility.
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http://dx.doi.org/10.1021/acsnano.1c03252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425335PMC
September 2021

Nanoparticle-Mediated Targeted Drug Delivery to Remodel Tumor Microenvironment for Cancer Therapy.

Int J Nanomedicine 2021 25;16:5811-5829. Epub 2021 Aug 25.

Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.

Advanced research has revealed the crucial role of tumor microenvironment (TME) in tumorigenesis. TME consists of a complicated network with a variety of cell types including endothelial cells, pericytes, immune cells, cancer-associated fibroblasts (CAFs), cancer stem cells (CSCs) as well as the extracellular matrix (ECM). The TME-constituting cells interact with the cancerous cells through plenty of signaling mechanisms and pathways in a dynamical way, participating in tumor initiation, progression, metastasis, and response to therapies. Hence, TME is becoming an attractive therapeutic target in cancer treatment, exhibiting potential research interest and clinical benefits. Presently, the novel nanotechnology applied in TME regulation has made huge progress. The nanoparticles (NPs) can be designed as demand to precisely target TME components and to inhibit tumor progression through TME modulation. Moreover, nanotechnology-mediated drug delivery possesses many advantages including prolonged circulation time, enhanced bioavailability and decreased toxicity over traditional therapeutic modality. In this review, update information on TME remodeling through NPs-based targeted drug delivery strategies for anticancer therapy is summarized.
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http://dx.doi.org/10.2147/IJN.S321416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403563PMC
August 2021

A Desolvation-Free Sodium Dual-Ion Chemistry for High Power Density and Extremely Low Temperature.

Angew Chem Int Ed Engl 2021 Aug 30. Epub 2021 Aug 30.

Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Institute of New Energy, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Fudan University, Shanghai, 200433, China.

The development of conventional rechargeable batteries based on intercalation chemistry in the fields of fast charge and low temperature is generally hindered by the sluggish cation-desolvation process at the electrolyte/electrode interphase. To address this issue, a novel desolvation-free sodium dual-ion battery (SDIB) has been proposed by using artificial graphite (AG) as anode and polytriphenylamine (PTPAn) as cathode. Combining the cation solvent co-intercalation and anion storage chemistry, such a SDIB operated with ether-based electrolyte can intrinsically eliminate the sluggish desolvation process. Hence, it can exhibit an extremely fast kinetics of 10 Ag (corresponding to 100C-rate) with a high capacity retention of 45 %. Moreover, the desolvation-free mechanism endows the battery with 61 % of its room-temperature capacity at an ultra-low temperature of -70 °C. This advanced battery system will open a door for designing energy storage devices that require high power density and a wide operational temperature range.
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http://dx.doi.org/10.1002/anie.202110501DOI Listing
August 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nat Commun 2021 08 26;12(1):5147. Epub 2021 Aug 26.

Institute of Systems Biomedicine, Department of Pathology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing, China.

PTEN is frequently mutated in human cancers and PTEN mutants promote tumor progression and metastasis. PTEN mutations have been implicated in immune regulation, however, the underlying mechanism is largely unknown. Here, we report that PTENα, the isoform of PTEN, remains active in cancer bearing stop-gained PTEN mutations. Through counteraction of CD8 T cell-mediated cytotoxicity, PTENα leads to T cell dysfunction and accelerates immune-resistant cancer progression. Clinical analysis further uncovers that PTENα-active mutations suppress host immune responses and result in poor prognosis in cancer as relative to PTENα-inactive mutations. Furthermore, germline deletion of Ptenα in mice increases cell susceptibility to immune attack through augmenting stress granule formation and limiting synthesis of peroxidases, leading to massive oxidative cell death and severe inflammatory damage. We propose that PTENα protects tumor from T cell killing and thus PTENα is a potential target in antitumor immunotherapy.
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http://dx.doi.org/10.1038/s41467-021-25417-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390757PMC
August 2021

Serine Metabolism Tunes Immune Responses To Promote Survival upon Infection.

mSystems 2021 Aug 24;6(4):e0042621. Epub 2021 Aug 24.

Center for Proteomics and Metabolomics, State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen Universitygrid.12981.33, Guangzhou, People's Republic of China.

Overactive immune response is a critical factor triggering host death upon bacterial infection. However, the mechanism behind the regulation of excessive immune responses is still largely unknown, and the corresponding control and preventive measures are still to be explored. In this study, we find that Nile tilapia, , that died from Edwardsiella tarda infection had higher levels of immune responses than those that survived. Such immune responses are strongly associated with metabolism that was altered at 6 h postinfection. By gas chromatography-mass spectrometry-based metabolome profiling, we identify glycine, serine, and threonine metabolism as the top three of the most impacted pathways, which were not properly activated in the fish that died. Serine is one of the crucial biomarkers. Exogenous serine can promote survival both as a prophylactic and therapeutic upon infection. Our further analysis revealed exogenous serine flux into the glycine, serine, and threonine metabolism and, more importantly, the glutathione metabolism via glycine. The increased glutathione synthesis could downregulate reactive oxygen species. Therefore, these data together suggest that metabolic modulation of immune responses is a potential preventive strategy to control overactive immune responses. Bacterial virulence factors are not the only factors responsible for host death. Overactive immune responses, such as cytokine storm, contribute to tissue injury that results in organ failure and ultimately the death of the host. Despite the recent development of anti-inflammation strategies, the way to tune immune responses to an appropriate level is still lacking. We propose that metabolic modulation is a promising approach in tuning immune responses. We find that the metabolomic shift at as early as 6 h postinfection can be predictive of the consequences of infection. Serine is a crucial biomarker whose administration can promote host survival upon bacterial infection either in a prophylactic or therapeutic way. Further analysis demonstrated that exogenous serine promotes the synthesis of glutathione, which downregulates reactive oxygen species to dampen immune responses. Our study exemplifies that the metabolite(s) is a potential therapeutic reagent for overactive immune response during bacterial infection.
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http://dx.doi.org/10.1128/mSystems.00426-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407201PMC
August 2021

Adsorption of inorganic and organic phosphorus onto polypyrrole modified red mud: Evidence from batch and column experiments.

Chemosphere 2021 Aug 13;286(Pt 3):131862. Epub 2021 Aug 13.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, 266033, PR China. Electronic address:

The ubiquitous presence of inorganic and organic phosphorus in wastewater and natural water bodies has deteriorated the water environment qualities and exerted significant influences on ecosystems. In this study, an effective polypyrrole modified red mud adsorbent (PRM) was optimized for the adsorptive removal of inorganic and organic phosphorus from aqueous solutions. The addition of ferric chloride and pyrrole was optimized for complete oxidation and modification of polypyrrole onto red mud. Kinetic studies illustrated that the adsorption progress was accomplished by physical and chemical adsorption. The experimental data of the optimized PRM were described well by Langmuir isotherm, and the equilibrium adsorption capacity was 32.9 and 54.7 mg/g for inorganic and organic phosphorus, respectively. The PRM showed commendable adsorption performance despite the pH conditions ranging from 3 to 11. From the effect of ion strength and X-ray photoelectron spectroscopy (XPS) tests, we found that ligand exchange is the main mechanism of orthophosphate adsorption onto PRM, while electrostatic attraction played an important role in organic phosphorus adsorption. The adsorption performance from column studies showed that the velocity of flow influenced the breakthrough time of the column but the initial concentration had minor impacts. This study would extend the potential application of polypyrrole modified red mud, acting as an efficient adsorbent for inorganic and organic phosphorus adsorption in water treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131862DOI Listing
August 2021

FK506 binding protein 10: a key actor of collagen crosslinking in clear cell renal cell carcinoma.

Aging (Albany NY) 2021 08 13;13(15):19475-19485. Epub 2021 Aug 13.

Department of Urology Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Clear cell renal cell carcinoma (ccRCC) is the most common type of malignant tumor in the kidney. With numbers of patients whose physical condition or tumor stage not suitable for radical surgery, they only have a narrow choice of using VEGF/mTOR targeted drugs to control their tumors, but ccRCC often shows resistance to these drugs. Therefore, identifying a new therapeutic target is of urgent necessity. In this study, for the first time, we concluded from bioinformatics analyses and research that FK506 binding protein 10 (FKBP10), together with its molecular partner Lysyl hydroxylase 2 (LH2/PLOD2), participate in the process of type I collagen synthesis in ccRCC via regulating crosslinking of pro-collagen chains. Our findings may provide a potential therapeutic target to treat ccRCC in the future.
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http://dx.doi.org/10.18632/aging.203359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386577PMC
August 2021

Determinants and dynamics of SARS-CoV-2 infection in a diverse population: 6-month evaluation of a prospective cohort study.

J Infect Dis 2021 Aug 13. Epub 2021 Aug 13.

Center for Advanced Biotechnology and Medicine; Rutgers University; Piscataway, NJ, USA.

Background: We studied risk factors, antibody responses, and symptoms of SARS-CoV-2 infection in a diverse, ambulatory population.

Methods: A prospective cohort (n=831, including 548 hospital-based healthcare workers) previously undiagnosed with SARS-CoV-2 infection was followed for six months with serial testing (SARS-CoV-2 PCR, specific IgG) and surveys.

Results: 93 participants (11.2%) tested SARS-CoV-2-positive; 14 (15.1%) were asymptomatic and 24 (25.8%), severely symptomatic. Healthcare workers were more likely to become infected (14.2% vs. 5.3%, aOR 2.1, 95% CI 1.4-3.3) and have severe symptoms (29.5% vs. 6.7%). IgG antibodies were detected after 79% of asymptomatic infections, 89% with mild-moderate symptoms, and 96% with severe symptoms. IgG trajectories after asymptomatic infection (slow increases) differed from symptomatic infections (early peaks within 2 months). Most participants (92%) had persistent IgG responses (median 171 days). In multivariable models, IgG titers were positively associated with symptom severity, certain comorbidities, and hospital work. Dyspnea, altered smell and taste, and other neurologic changes persisted for ≥120 days in ≥10% of affected participants. Participants with prolonged symptoms (generally more severely symptomatic) had higher antibody levels.

Conclusions: In a prospective, ethnically diverse cohort, symptom severity correlated with the magnitude and trajectory of IgG production. Symptoms frequently persisted for many months after infection.
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http://dx.doi.org/10.1093/infdis/jiab411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436370PMC
August 2021

Pseudorabies virus inhibits type I and type III interferon-induced signaling via proteasomal degradation of Janus kinases.

J Virol 2021 Aug 11:JVI0079321. Epub 2021 Aug 11.

Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.

Both type I and III interferons (IFNs) play a crucial role in host antiviral response by activating the JAK/STAT (Janus kinase/signal transducer and activator of transcription) signaling pathway to trigger the expression of antiviral IFN-stimulated genes (ISGs). We report that the porcine alphaherpesvirus pseudorabies virus (PRV) triggers proteasomal degradation of the key Janus kinases Jak1 and to a lesser exent Tyk2, thereby inhibiting both type I and III IFN-induced STAT1 phosphorylation and suppressing IFN-induced expression of ISGs. UV-inactivated PRV did not interfere with IFN signaling. In addition, deletion of the EP0 gene from the PRV genome or inhibition of viral genome replication did not affect PRV-induced inhibition of IFN signaling. To our knowledge, this is the first report describing Janus kinase degradation by alphaherpesviruses. These findings thus reveal a novel alphaherpesvirus evasion mechanism of type I and type III IFNs. Type I and III IFNs trigger signaling via Janus kinases that phosphorylate and activate STAT transcription factors, leading to the expression of antiviral interferon-stimulated genes (ISGs) that result in an antiviral state of host cells. Viruses have evolved various mechanisms to evade this response. Our results indicate that an alphaherpesvirus, the porcine pseudorabies virus (PRV), inhibits both type I and III IFNs signaling pathways by triggering proteasome-dependent degradation of the key Janus kinases Jak1 and Tyk2 and consequent inhibition of STAT1 phosphorylation and suppression of ISG expression. Moreover, we found that this inhibition is not caused by incoming virions and does not depend on expression of the viral EP0 protein or viral true late proteins. These data for the first time address alphaherpesvirus evasion of type III IFN-mediated signaling and reveal a previously uncharacterized alphaherpesvirus mechanism of IFN evasion via proteasomal degradation of Janus kinases.
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http://dx.doi.org/10.1128/JVI.00793-21DOI Listing
August 2021

Identification and Behavioral Assays of Alarm Pheromone in the Vetch Aphid Megoura viciae.

J Chem Ecol 2021 Aug 4. Epub 2021 Aug 4.

Department of Entomology and MOA Key Laboratory for Monitoring and Environment-Friendly Control of Crop Pests, College of Plant Protection, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100193, China.

Aphids are destructive pests, and alarm pheromones play a key role in their chemical ecology. Here, we conducted a detailed analysis of terpenoids in the vetch aphid, Megoura viciae, and its host plant Pisum sativum using gas chromatography/mass spectrometry. Four major components, (-)-β-pinene (49.74%), (E)-β-farnesene (32.64%), (-)-α-pinene (9.42%) and ( +)-limonene (5.24%), along with trace amounts of ( +)-sabinene, camphene and α-terpineol) (3.14%) were found in the aphid. In contrast, few terpenoids were found in the host plant, consisting mainly of squalene (66.13%) and its analog 2,3-epoxysqualene (31.59%). Quantitative analysis of the four major terpenes in different developmental stages of the aphid showed that amounts of the monoterpenes increased with increasing stage, while the sesquiterpene amount peaked in the 3 instar. (-)-β-Pinene was the most abundant terpene at all developmental stages. Behavioral assays using a three-compartment olfactometer revealed that the repellency of single compounds varied in a concentration-dependent manner, but two mixtures [(-)-α-pinene: (-)-β-pinene: (E)-β-farnesene: ( +)-limonene = 1:44.4:6.5:2.2 or 1:18.4:1.3:0.8], were repellent at all concentrations tested. Our results suggest that (-)-α-pinene and (-)-β-pinene are the major active components of the alarm pheromone of M. viciae, but that mixtures play a key role in the alarm response. Our study contributes to the understanding of the chemical ecology of aphids and may help design new control strategies against this aphid pest.
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http://dx.doi.org/10.1007/s10886-021-01297-4DOI Listing
August 2021

Apelin receptor homodimer inhibits apoptosis in vascular dementia.

Exp Cell Res 2021 Oct 31;407(1):112739. Epub 2021 Jul 31.

School of Basic Medical Sciences, Weifang Medical University, Weifang, 261053, China. Electronic address:

Apelin receptor (APJ), a member of family A of the G protein-coupled receptors (GPCRs), is a potential pharmaceutical target for diseases of the nervous system. Our previous work revealed that human APJ can form a homodimer that has different functional characteristics than the monomer. To investigate the effects of APJ homodimers on neuroprotection in vascular dementia (VD), we established VD model in rats and treated the animals by injecting apelin-13 into the lateral ventricle. In addition, we established an oxygen-glucose deprivation/reoxygenation (OGD/R) model in SH-SY5Y cells treated with apelin-13. After apelin-13 stimulation in the VD rat, the level of APJ and APJ homodimer were elevated. Furthermore, APJ homodimer decreased the level of cleaved caspase-3 and cleaved caspase-9 via the Gα and Gα signaling pathway, thereby increasing the number of neurons and inhibiting apoptosis. Consequently, APJ homodimers may serve as a unique mechanism for neuroprotection against VD and provide new pharmaceutical targets for VD.
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http://dx.doi.org/10.1016/j.yexcr.2021.112739DOI Listing
October 2021

Van der Waals epitaxy of nearly single-crystalline nitride films on amorphous graphene-glass wafer.

Sci Adv 2021 Jul 30;7(31). Epub 2021 Jul 30.

Research and Development Center for Semiconductor Lighting Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Van der Waals epitaxy provides a fertile playground for the monolithic integration of various materials for advanced electronics and optoelectronics. Here, a previously unidentified nanorod-assisted van der Waals epitaxy is developed and nearly single-crystalline GaN films are first grown on amorphous silica glass substrates using a graphene interfacial layer. The epitaxial GaN-based light-emitting diode structures, with a record internal quantum efficiency, can be readily lifted off, becoming large-size flexible devices. Without the effects of the potential field from a single-crystalline substrate, we expect this approach to be equally applicable for high-quality growth of nitrides on arbitrary substrates. Our work provides a revolutionary technology for the growth of high-quality semiconductors, thus enabling the hetero-integration of highly mismatched material systems.
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http://dx.doi.org/10.1126/sciadv.abf5011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324058PMC
July 2021

A 2.63 μW ECG Processor With Adaptive Arrhythmia Detection and Data Compression for Implantable Cardiac Monitoring Device.

IEEE Trans Biomed Circuits Syst 2021 Aug 15;15(4):777-790. Epub 2021 Sep 15.

An ultra-low power ECG processor ASIC (application specific integrated circuit) with R-wave detection and data compression is presented, which is designed for the long-term implantable cardiac monitoring (ICM) device for arrhythmia diagnosis. An adaptive derivative-based detection algorithm with low computation overhead for potential arrhythmia recording is proposed to detect arrhythmia with the occasional abnormal heart beats. In order to save as much as possible cardiac information with the limited memory size available in the ICM device, a hierarchical data buffer structure is proposed which saves 3 types of data, including the raw ECG data segments of 2 seconds, compressed ECG data segments of 45 seconds, and R-peak values and interval lengths of >2000 beat cycles. A modified swinging-door-trending (SDT) method is proposed for the ECG data compression. The ASIC has been implemented based on fully-customized near-threshold standard cells using the thick-gate transistors in 65-nm CMOS technology for low dynamic power consumption and leakage. The ASIC core occupies a die area of 1.77 mm. The measured total power is 2.63 μW, which is among the ECG processors with the lowest core power consumption. It exhibits a relatively high positive precision rate (P) of 99.3% with a sensitivity of 98.2%, in contrast to the similar designs in literature with the same core power consumption level. Also, an ECG data compression ratio (CR) of up to 17.0 has been achieved, with a good trade-off between the compression efficiency and loss.
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http://dx.doi.org/10.1109/TBCAS.2021.3100434DOI Listing
August 2021

A consensus and saturated genetic map provides insight into genome anchoring, synteny of Solanaceae and leaf- and fruit-related QTLs in wolfberry (Lycium Linn.).

BMC Plant Biol 2021 Jul 24;21(1):350. Epub 2021 Jul 24.

Wolfberry Science Research Institute, Ningxia Academy of Agriculture and Forestry Sciences/National Wolfberry Engineering Research Center, Yinchuan, 750002, China.

Background: Lycium Linn. (Solanaceae) is a genus of economically important plants producing fruits and leaves with high nutritional value and medicinal benefits. However, genetic analysis of this plant and molecular breeding for quality improvement are limited by the lack of sufficient molecular markers.

Results: In this study, two parental strains, 'Ningqi No. 1' (Lycium barbarum L.) and 'Yunnan Gouqi' (Lycium yunnanense Kuang et A.M. Lu), and 200 F hybrid individuals were resequenced for genetic analysis. In total, 8,507 well-selected SNPs were developed, and a high-density genetic map (NY map) was constructed with a total genetic distance of 2,122.24 cM. A consensus genetic map was established by integrating the NY map and a previously published genetic map (NC map) containing 15,240 SNPs, with a total genetic distance of 3,058.19 cM and an average map distance of 0.21 cM. The 12 pseudochromosomes of the Lycium reference genome were anchored using this consensus genetic map, with an anchoring rate of 64.3%. Moreover, weak collinearities between the consensus map and the pepper, potato, and tomato genomes were observed. Twenty-five stable QTLs were identified for leaf- and fruit-related phenotypes, including fruit weight, fruit longitude, leaf length, the fruit index, and the leaf index; these stable QTLs were mapped to four different linkage groups, with LOD scores ranging from 2.51 to 19.37 and amounts of phenotypic variance explained from 6.2% to 51.9%. Finally, 82 out of 188 predicted genes underlying stable QTLs for fruit-related traits were differentially expressed according to RNA-seq analysis.

Conclusions: A chromosome-level assembly can provide a foundation for further functional genomics research for wolfberry. The genomic regions of these stably expressed QTLs could be used as targets for further fine mapping and development of molecular markers for marker-assisted selection (MAS). The present study provided valuable information on saturated SNP markers and reliable QTLs for map-based cloning of functional genes related to yield and morphological traits in Lycium spp.
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http://dx.doi.org/10.1186/s12870-021-03115-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306383PMC
July 2021

UIO-66 as Nucleating Agent on the Crystallization Behavior and Properties of Poly(Ethylene Terephthalate).

Polymers (Basel) 2021 Jul 10;13(14). Epub 2021 Jul 10.

Department of Polymer Materials and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

In this study, not only was the similar terephthalate structure between UIO-66 and PET utilized to improve compatibility, but the Zr exposed by defects of UIO-66 was also utilized to improve the interaction between PET and UIO-66. Furthermore, PET nanocomposites with different contents of UIO-66 were also fabricated. Due to the high specific surface area and coordination of Zr, UIO-66 has high nucleation efficiency in the PET matrix. Compared with pure PET, the crystallization rate of PET/UIO-66 nanocomposite is significantly increased, and the crystallization temperature of PET-UIO66-1 is significantly increased from 194.3 °C to 211.6 °C. In addition, the tensile strength of nanocomposites has also been improved due to coordination.
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http://dx.doi.org/10.3390/polym13142266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309308PMC
July 2021

Dissecting the chromosome-level genome of the Asian Clam (Corbicula fluminea).

Sci Rep 2021 Jul 22;11(1):15021. Epub 2021 Jul 22.

Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China.

The Asian Clam (Corbicula fluminea) is a valuable commercial and medicinal bivalve, which is widely distributed in East and Southeast Asia. As a natural nutrient source, the clam is rich in protein, amino acids, and microelements. The genome of C. fluminea has not yet been characterized; therefore, genome-assisted breeding and improvements cannot yet be implemented. In this work, we present a de novo chromosome-scale genome assembly of C. fluminea using PacBio and Hi-C sequencing technologies. The assembled genome comprised 4728 contigs, with a contig N50 of 521.06 Kb, and 1,215 scaffolds with a scaffold N50 of 70.62 Mb. More than 1.51 Gb (99.17%) of genomic sequences were anchored to 18 chromosomes, of which 1.40 Gb (92.81%) of genomic sequences were ordered and oriented. The genome contains 38,841 coding genes, 32,591 (83.91%) of which were annotated in at least one functional database. Compared with related species, C. fluminea had 851 expanded gene families and 191 contracted gene families. The phylogenetic tree showed that C. fluminea diverged from Ruditapes philippinarum, ~ 228.89 million years ago (Mya), and the genomes of C. fluminea and R. philippinarum shared 244 syntenic blocks. Additionally, we identified 2 MITF members and 99 NLRP members in C. fluminea genome. The high-quality and chromosomal Asian Clam genome will be a valuable resource for a range of development and breeding studies of C. fluminea in future research.
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http://dx.doi.org/10.1038/s41598-021-94545-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298618PMC
July 2021

Maternal cecal microbiota transfer rescues early-life antibiotic-induced enhancement of type 1 diabetes in mice.

Cell Host Microbe 2021 Aug 21;29(8):1249-1265.e9. Epub 2021 Jul 21.

Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ, USA; Human Microbiome Program, New York University Langone Medical Center, New York, NY, USA. Electronic address:

Early-life antibiotic exposure perturbs the intestinal microbiota and accelerates type 1 diabetes (T1D) development in the NOD mouse model. Here, we found that maternal cecal microbiota transfer (CMT) to NOD mice after early-life antibiotic perturbation largely rescued the induced T1D enhancement. Restoration of the intestinal microbiome was significant and persistent, remediating the antibiotic-depleted diversity, relative abundance of particular taxa, and metabolic pathways. CMT also protected against perturbed metabolites and normalized innate and adaptive immune effectors. CMT restored major patterns of ileal microRNA and histone regulation of gene expression. Further experiments suggest a gut-microbiota-regulated T1D protection mechanism centered on Reg3γ, in an innate intestinal immune network involving CD44, TLR2, and Reg3γ. This regulation affects downstream immunological tone, which may lead to protection against tissue-specific T1D injury.
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http://dx.doi.org/10.1016/j.chom.2021.06.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370265PMC
August 2021

A novel preoperative predictive model of 90-day mortality after liver resection for huge hepatocellular carcinoma.

Ann Transl Med 2021 May;9(9):774

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion (Fudan University), Ministry of Education; Shanghai Key Laboratory of Organ Transplantation, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Hepatectomy for huge hepatocellular carcinoma (HCC) (diameter ≥10 cm) is characterized by high mortality. This study aimed to establish a preoperative model to evaluate the risk of postoperative 90-day mortality for huge HCC patients.

Methods: We retrospectively enrolled 1,127 consecutive patients and prospectively enrolled 93 patients with huge HCC who underwent hepatectomy (training cohort, n=798; validation cohort, n=329; prospective cohort, n=93) in our institute. Based on independent preoperative predictors of 90-day mortality, we established a logistic regression model and visualized the model by nomogram.

Results: The 90-day mortality rates were 9.6%, 9.2%, and 10.9% in the training, validation, and prospective cohort. The α-fetoprotein (AFP) level, the prealbumin levels, and the presence of portal vein tumor thrombosis (PVTT) were preoperative independent predictors of 90-day mortality. A logistic regression model, AFP-prealbumin-PVTT score (APP score), was subsequently established and showed good performance in predicting 90-day mortality (training cohort, AUC =0.87; validation cohort, AUC =0.91; prospective cohort, AUC =0.93). Using a cut-off of -1.96, the model could stratify patients into low risk (≤-1.96) and high risk (>-1.96) with different 90-day mortality rates (~30% ~2%). Furthermore, the predictive performance for 90-day mortality and overall survival was significantly superior to the Child-Pugh score, the model of end-stage liver disease (MELD) score, and the albumin-bilirubin (ALBI) score.

Conclusions: The APP score can precisely predict postoperative 90-day mortality as well as long-term survival for patients with huge HCC, assisting physician selection of suitable candidates for liver resection and improving the safety and efficacy of surgical treatment.
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http://dx.doi.org/10.21037/atm-20-7842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246173PMC
May 2021

HIPK2 phosphorylates HDAC3 for NF-κB acetylation to ameliorate colitis-associated colorectal carcinoma and sepsis.

Proc Natl Acad Sci U S A 2021 Jul;118(28)

Institute of Geriatrics, Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Shanghai 200444, China;

Although inflammation is critical for the clearance of pathogens, uncontrolled inflammation also contributes to the development of multiple diseases such as cancer and sepsis. Since NF-κB-mediated transactivation in the nucleus is pivotal downstream of various stimuli to induce inflammation, searching the nuclear-localized targets specifically regulating NF-κB activation will provide important therapeutic application. Here, we have identified that homeodomain-interacting protein kinase 2 (HIPK2), a nuclear serine/threonine kinase, increases its expression in inflammatory macrophages. Importantly, HIPK2 deficiency or overexpression could enhance or inhibit inflammatory responses in LPS-stimulated macrophages, respectively. HIPK2-deficient mice were more susceptible to LPS-induced endotoxemia and CLP-induced sepsis. Adoptive transfer of bone marrow cells (BMs) also aggravated AOM/DSS-induced colorectal cancer. Mechanistically, HIPK2 bound and phosphorylated histone deacetylase 3 (HDAC3) at serine 374 to inhibit its enzymatic activity, thus reducing the deacetylation of p65 at lysine 218 to suppress NF-κB activation. Notably, the HDAC3 inhibitors protected wild-type or BMs-reconstituted mice from LPS-induced endotoxemia. Our findings suggest that the HIPK2-HDAC3-p65 module in macrophages restrains excessive inflammation, which may represent a new layer of therapeutic mechanism for colitis-associated colorectal cancer and sepsis.
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http://dx.doi.org/10.1073/pnas.2021798118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285910PMC
July 2021

[Oral Mucosal Diffuse Large B-cell Lymphoma Caused by Long-term Oral Methotrexate:Report of One Case].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Jun;43(3):488-493

Department of Hematology, Fujian Medical University Union Hospital,Fuzhou 350001,China.

A case of primary oral mucosal diffuse large B-cell lymphoma(DLBCL)due to long-term use of methotrexate(MTX)for the treatment of rheumatoid arthritis(RA)was admitted to the Department of Hematology,Fujian Medical University Union Hospital.We analyzed and discussed the clinical features,diagnosis and treatment,and prognosis of specific malignant lymphoma induced by MTX in this RA patient.Our purpose is to improve the awareness and knowledge of other iatrogenic immunodeficiency-associated lymphoproliferative disorders of clinicians and pathologists.This study provides a new reference for the clinical diagnosis and treatment of MTX-associated DLBCL.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13717DOI Listing
June 2021

Milrinone Ameliorates the Neuroinflammation and Memory Function of Alzheimer's Disease in an APP/PS1 Mouse Model.

Neuropsychiatr Dis Treat 2021 30;17:2129-2139. Epub 2021 Jun 30.

College of Medical Technology, Qiqihar Medical University, Qiqihar City, Heilongjiang Province, 161000, People's Republic of China.

Purpose: Alzheimer's disease (AD) is a complex neurodegenerative disorder, which is characterized by memory loss and cognitive deficits. The neuroprotective role of milrinone on the injury of spinal cord or cerebral ischemia-reperfusion has been confirmed. However, the accurate function of milrinone on AD pathogeny is still unclear.

Methods: APP/PS1 transgenic mouse was used to explore the role of milrinone in behaviour tests, and the effects on histopathologic features of AD such as the formation of neuronal amyloid-β (Aβ) plaque, microglial activation, tau protein hyperphosphorylation, oxidative stress, and neuroinflammation. Lipopolysaccharide (LPS)/Aβ-treated BV-2 cells were used to understand the anti-inflammation mechanism of milrinone on AD in vitro.

Results: Our in vivo results showed that milrinone ameliorates the memory functions of AD mice. Meanwhile, milrinone reduced Aβ deposits, repressed microglial activation and tau protein hyperphosphorylation, attenuated the oxidative stress, and decreased the levels of inflammatory cytokines. The in vitro results demonstrated that milrinone could inhibit the secretion of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α via regulation of NLRP3 inflammasomes and TLR4/MyD88/NF-κB signalling pathway.

Conclusion: Overall, milrinone could ameliorate the memory loss and cognitive deficits through repressing the multiple pathological processes of AD, suggesting that milrinone may be an underlying and effective drug for treating AD clinically.
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http://dx.doi.org/10.2147/NDT.S312648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256386PMC
June 2021

Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating the SIRT2-p65 pathway.

Front Med 2021 Jun 28. Epub 2021 Jun 28.

Department of Cardiovascular Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

Exposure to particulate matter 2.5 (PM2.5) potentially triggers airway inflammation by activating nuclear factor-κB (NF-κB). Sirtuin 2 (SIRT2) is a key modulator in inflammation. However, the function and specific mechanisms of SIRT2 in PM2.5-induced airway inflammation are largely understudied. Therefore, this work investigated the mechanisms of SIRT2 in regulating the phosphorylation and acetylation of p65 influenced by PM2.5-induced airway inflammation and bronchial hyperresponsiveness. Results revealed that PM2.5 exposure lowered the expression and activity of SIRT2 in bronchial tissues. Subsequently, SIRT2 impairment promoted the phosphorylation and acetylation of p65 and activated the NF-κB signaling pathway. The activation of p65 triggered airway inflammation, increment of mucus secretion by goblet cells, and acceleration of tracheal stenosis. Meanwhile, p65 phosphorylation and acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice exposed to PM2.5. Triptolide (a specific p65 inhibitor) reversed p65 activation and ameliorated PM2.5-induced airway inflammation and bronchial hyperresponsiveness. Our findings provide novel insights into the molecular mechanisms underlying the toxicity of PM2.5 exposure. Triptolide inhibition of p65 phosphorylation and acetylation could be an effective therapeutic approach in averting PM2.5-induced airway inflammation and bronchial hyperresponsiveness.
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http://dx.doi.org/10.1007/s11684-021-0839-4DOI Listing
June 2021

Retrospective Analysis of Esophageal Foreign Body Ingestion: Differences Among Weekday, Weekends, and Holidays.

Risk Manag Healthc Policy 2021 15;14:2499-2506. Epub 2021 Jun 15.

Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, People's Republic of China.

Purpose: Current data on the occurrence of EFBs in relation to different time periods are scant. The purpose of this study was to compare the occurrence, type and location of EFBs in people who presented at our center on weekdays, weekends and holidays.

Patients And Methods: A retrospective analysis of patients (n=1353) received rigid esophagoscopy for foreign body extraction under general anesthesia from January 2018 to 2020 December.

Results: The occurrence of EFB ingestion was significantly higher (p<0.05) on holidays (2.75/day) than on weekdays (0.91/day) and weekends (1.46/day). Patients were much older on holidays, with a mean age of 58.18 ± 17.34 years, than patients on weekdays (54.71 ± 16.71 years) and weekends (55.05 ± 17.36 years).

Conclusion: Our findings demonstrate that EFB ingestion is most likely to occur on weekends and holidays, and elderly patients should be advised to chewing slowly and eating non-nuclear jujube, especially during holidays, to minimize their risk.
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http://dx.doi.org/10.2147/RMHP.S314069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214566PMC
June 2021

A T-shaped gate tunneling field effect transistor with negative capacitance, super-steep subthreshold swing.

Nanotechnology 2021 Jul 9;32(39). Epub 2021 Jul 9.

Northwestern Polytechnical University, School of Microelectronics, Xi'an, People's Republic of China.

With the development of semiconductor technology, the size of traditional metal oxide semiconductor field effect transistor devices continues to decrease, but it cannot meet the requirements of high performance and low power consumption. Low power tunneling field effect transistor (TFET) has gradually become the focus of researchers. This paper proposes a novel T-shaped gate TFET based on the silicon with the negative capacitance (NC-TGTFET). On the basis of TGTFET, ferroelectric material (HZO) is used as gate dielectric. The simulation results show that, compared with the traditional TGTFET, the opening order and sensitivity of the two tunneling junctions are different. The influences of thickness and the doping concentration of pocket and ferroelectric material properties on the characteristics of NC-TGTFET is also discussed by Sentaurus simulation tool. Furthermore, the negative capacitance of ferroelectric material makes NC-TGTFET have a very steep subthreshold swing (18.32 mV/dec) at the range of drain current from 1 × 10to 1 × 10Am. And the on-state current (= 0.5 V,= 0.5 V) is 1.52 × 10Am.
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http://dx.doi.org/10.1088/1361-6528/ac0d20DOI Listing
July 2021

MMP-7 affects peritoneal ultrafiltration associated with elevated aquaporin-1 expression via MAPK/ERK pathway in peritoneal mesothelial cells.

J Cell Mol Med 2021 Jul 11;25(14):6887-6898. Epub 2021 Jun 11.

Division of Nephrology, State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Peritoneal membrane dysfunction and the resulting ultrafiltration failure are the major disadvantages of long-term peritoneal dialysis (PD). It becomes increasingly clear that mesothelial cells play a vital role in the pathophysiological changes of the peritoneal membrane. Matrix metalloproteinases (MMPs) function in the extracellular environment of cells and mediate extracellular matrix turnover during peritoneal membrane homeostasis. We showed here that dialysate MMP-7 levels markedly increased in the patients with PD, and the elevated MMP-7 level was negatively associated with peritoneal ultrafiltration volume. Interestingly, MMP-7 could regulate the cell osmotic pressure and volume of human peritoneal mesothelial cells. Moreover, we provided the evidence that MMP-7 activated mitogen-activated protein kinases (MAPKs)-extracellular signal-regulated kinase 1/2 (ERK) pathway and subsequently promoted the expression of aquaporin-1 (AQP-1) resulting in the change of cell osmotic pressure. Using a specific inhibitor of ERK pathway abrogated the MMP-7-mediating AQP-1 up-regulation and cellular homeostasis. In summary, all the findings indicate that MMP-7 could modulate the activity of peritoneal cavity during PD, and dialysate MMP-7 might be a non-invasive biomarker and an alternative therapeutic target for PD patients with ultrafiltration failure.
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http://dx.doi.org/10.1111/jcmm.16697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278116PMC
July 2021

Colloidal gold immunochromatographic assay (GICA) is an effective screening method for identifying detectable anti-SARS-CoV-2 neutralizing antibodies.

Int J Infect Dis 2021 Jul 6;108:483-486. Epub 2021 Jun 6.

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University, Beijing 100191, China. Electronic address:

Introduction: A large number of COVID-19 patients are in recovery, and millions of people are vaccinated for COVID-19 globally. This calls for a rapid screening strategy of SARS-CoV-2 protective antibodies, generated in rehabilitated and vaccinated populations.

Methods: Serum samples collected over a follow-up period of six months from 306 COVID-19 cases discharged from Wuhan Tongji Hospital were analyzed. Anti-S Abs were detected by colloidal gold immunochromatographic assay (GICA), and neutralizing antibodies (nAbs) were detected by chemiluminescent microparticle immunoassay (CMIA).

Results: Most COVID-19 survivors tested positive for anti-S Abs (83.7%) and nAbs (98.0%) 6 months after being discharged from the hospital, and the levels of anti-S Abs in the blood were highly positively correlated with nAbs (r = 0.652, P < 0.0001). The positivity rate of nAbs for patients with anti-S Abs positive was 100%.

Conclusions: There is a good agreement between anti-S Abs detected by GICA and nAbs detected by CMIA. It indicates that anti-S Abs detected by GICA may be used as a cheaper screening strategy for detectable SARS-CoV-2 nAbs in COVID-19 convalescent individuals.
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http://dx.doi.org/10.1016/j.ijid.2021.05.080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180344PMC
July 2021
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