Publications by authors named "Yue Yang"

1,186 Publications

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A systematic review of automatic text summarization for biomedical literature and EHRs.

J Am Med Inform Assoc 2021 Aug 2. Epub 2021 Aug 2.

Carolina Health Informatics Program, University of North Carolina, Chapel Hill, North Carolina, USA.

Objective: Biomedical text summarization helps biomedical information seekers avoid information overload by reducing the length of a document while preserving the contents' essence. Our systematic review investigates the most recent biomedical text summarization researches on biomedical literature and electronic health records by analyzing their techniques, areas of application, and evaluation methods. We identify gaps and propose potential directions for future research.

Materials And Methods: This review followed the PRISMA methodology and replicated the approaches adopted by the previous systematic review published on the same topic. We searched 4 databases (PubMed, ACM Digital Library, Scopus, and Web of Science) from January 1, 2013 to April 8, 2021. Two reviewers independently screened title, abstract, and full-text for all retrieved articles. The conflicts were resolved by the third reviewer. The data extraction of the included articles was in 5 dimensions: input, purpose, output, method, and evaluation.

Results: Fifty-eight out of 7235 retrieved articles met the inclusion criteria. Thirty-nine systems used single-document biomedical research literature as their input, 17 systems were explicitly designed for clinical support, 47 systems generated extractive summaries, and 53 systems adopted hybrid methods combining computational linguistics, machine learning, and statistical approaches. As for the assessment, 51 studies conducted an intrinsic evaluation using predefined metrics.

Discussion And Conclusion: This study found that current biomedical text summarization systems have achieved good performance using hybrid methods. Studies on electronic health records summarization have been increasing compared to a previous survey. However, the majority of the works still focus on summarizing literature.
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http://dx.doi.org/10.1093/jamia/ocab143DOI Listing
August 2021

ZNF326 promotes colorectal cancer epithelial-mesenchymal transition.

Pathol Res Pract 2021 Jul 22;225:153554. Epub 2021 Jul 22.

Department of Pathology, College of Basic Medical Science, and the First Affiliated Hospital of China Medical University, Shenyang, China. Electronic address:

Zinc-finger protein 326 (ZNF326) activity has been reported in different tumors, but its expression and possible mechanism of action in colorectal cancer are not known. In this study, we applied immunohistochemistry to detect the expression of ZNF326 in colorectal tissues. Next, we used a ZNF326 expression plasmid and small interfering (si) RNA-ZNF326 (siZNF326) to transfect colorectal cancer cell lines in order to determine the effect of ZNF326 on cell migration and as well as its potential role in promoting epithelial-mesenchymal transition (EMT). A higher ZNF326 expression in the nuclei of colorectal tumor cells compared to normal mucosa was observed (70.3%, 109/155 specimens vs. 23.2%, 36/155 specimens). A high ZNF326 expression level was positively correlated with tumor differentiation, tumor-node-metastasis (TNM) staging, and lymph node metastasis. Transfection of cancer cell lines (SW480 and SW620) with a ZNF326-overexpression vector promoted colorectal cancer cell invasion and altered the expression of EMT-related proteins. Vimentin, N-cadherin, Snail, and Slug were upregulated, whereas E-cadherin and zonula occludens-1 (ZO-1) were downregulated. In contrast, downregulation of ZNF326 expression using siRNA-ZNF326 in cancer cell lines (CL187 and RKO) resulted in the opposite findings. ZNF326 overexpression also upregulated the expression of latent transforming growth factor beta binding protein 4 (LTBP4) and p-Smad2/3. In conclusion, ZNF326 promoted the EMT and invasiveness of colorectal cancer cells. These findings are likely due to LTBP4 and p-Smad2/3 upregulation and, in turn, transforming growth factor beta (TGF-β) signaling activation.
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http://dx.doi.org/10.1016/j.prp.2021.153554DOI Listing
July 2021

Positive charge in the complementarity-determining regions of synthetic nanobody prevents aggregation.

Biochem Biophys Res Commun 2021 Jul 28;572:1-6. Epub 2021 Jul 28.

Department of R&D, Origincell Therapeutics, Origincell Industrial Park, No. 1227 Zhangheng Road, Pudong New District, Shanghai, China. Electronic address:

In the past, specificity and affinity were the priority for synthetic antibody library. However, therapeutic antibodies need good stability for medical use. Through carefully adjust the chemical diversity in CDRs, one hopes to design a synthetic antibody library with good developability. Here we thoroughly analyzed 296 nanobody sequences and structures, constructed a fully-functional synthetic nanobody library, evaluated the relationship between aggregation and isoelectric point, and found that high-pI nanobodies were more resistant to aggregation than low-pIs. As we used the same framework for constructing the library, CDRs charge played a crucial role in mediating nanobody aggregation. We also analyzed the theoretical pI of 296 nanobodies from PDB, about 75% had basic pI, only 25% were acidic. Those results provided useful guidelines for designing next-generation synthetic nanobody libraries and for identifying potent and safe nanobody therapeutics.
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http://dx.doi.org/10.1016/j.bbrc.2021.07.054DOI Listing
July 2021

NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

Ecotoxicol Environ Saf 2021 Jul 28;223:112554. Epub 2021 Jul 28.

Department of Occupational Health, Army Medical University, 400038, Chongqing, People's Republic of China. Electronic address:

Arsenic is one of the most common environmental pollutants. Neurotoxicity induced by arsenic has become a major public health concern. However, the effects of arsenic-induced neurotoxicity in the brain and the underlying molecular mechanisms are not well understood. N-acetyl-cysteine (NAC) is a thiol-based antioxidant that can antagonize heavy metal-induced neurotoxicity by scavenging reactive oxygen species (ROS). Here, we used the mouse oligodendrocyte precursor cell (OPC) line Oli-neu to explore the neurotoxic effects of arsenic and the protective effects of NAC. We found that arsenic exposure decreased cell viability, increased oxidative stress, caused mitochondrial dysfunction, and led to apoptosis of Oli-neu cells. Furthermore, we revealed that NAC treatment reversed these neurotoxic effects of arsenic. TMEM179, a key membrane protein, was found highly expressed in OPCs and to be an important factor in maintaining mitochondrial functions. We found that TMEM179 played a critical role in mediating the neurotoxic effects of arsenic and the protective role of NAC. PKCβ is a downstream factor through which TMEM179 regulates the expression of apoptosis-related proteins. This study improves our understanding of the neurotoxic effects and mechanisms of arsenic exposure and the protective effects of NAC. It also identifies a potential molecular target, TMEM179, for the treatment of arsenic-induced neurotoxicity.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112554DOI Listing
July 2021

Discovery of Small-Molecule Inhibitors of SARS-CoV-2 Proteins Using a Computational and Experimental Pipeline.

Front Mol Biosci 2021 9;8:678701. Epub 2021 Jul 9.

Lawrence Livermore National Laboratory, Computing Directorate, Global Security Computing Division, Livermore, CA, United States.

A rapid response is necessary to contain emergent biological outbreaks before they can become pandemics. The novel coronavirus (SARS-CoV-2) that causes COVID-19 was first reported in December of 2019 in Wuhan, China and reached most corners of the globe in less than two months. In just over a year since the initial infections, COVID-19 infected almost 100 million people worldwide. Although similar to SARS-CoV and MERS-CoV, SARS-CoV-2 has resisted treatments that are effective against other coronaviruses. Crystal structures of two SARS-CoV-2 proteins, spike protein and main protease, have been reported and can serve as targets for studies in neutralizing this threat. We have employed molecular docking, molecular dynamics simulations, and machine learning to identify from a library of 26 million molecules possible candidate compounds that may attenuate or neutralize the effects of this virus. The viability of selected candidate compounds against SARS-CoV-2 was determined experimentally by biolayer interferometry and FRET-based activity protein assays along with virus-based assays. In the pseudovirus assay, imatinib and lapatinib had IC values below 10 μM, while candesartan cilexetil had an IC value of approximately 67 µM against M in a FRET-based activity assay. Comparatively, candesartan cilexetil had the highest selectivity index of all compounds tested as its half-maximal cytotoxicity concentration 50 (CC) value was the only one greater than the limit of the assay (>100 μM).
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http://dx.doi.org/10.3389/fmolb.2021.678701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315004PMC
July 2021

Baseline serum hepatitis B core antibody level predicts HBeAg seroconversion in patients with HBeAg-positive chronic hepatitis B after antiviral treatment.

Antiviral Res 2021 Jul 24;193:105146. Epub 2021 Jul 24.

Department of Infectious Diseases, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China; Department of Infectious Diseases, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address:

Antibody to hepatitis B core antigen (anti-HBc) is one of the most classical serological markers of HBV infection. This study aimed to investigate the association of serum anti-HBc and HBeAg seroconversion in patients with HBeAg-positive chronic hepatitis B (CHB) after antiviral treatment. Two hundred and seventeen HBeAg-positive CHB patients treated with entecavir (ETV) or tenofovir disoproxil fumarate (TDF) for 48 weeks were retrospectively enrolled. Serological response (SR) is defined as HBeAg seroconversion at 48 weeks of antiviral treatment. Serum anti-HBc level was measured using the Abbott ARCHITECT assay. After 48 weeks of antiviral treatment, twenty-two (10.1 %) patients achieved SR. Baseline level of serum anti-HBc in the SR patients (11.8 S/CO) was significantly higher than patients with non-SR (9.6 S/CO, P < 0.001). The median anti-HBc level was significantly declined after 48 weeks of antiviral therapy (9.9 vs. 8.9 S/CO, P < 0.001). Multivariate logistic regression analysis showed baseline of serum anti-HBc was an independent predictor of SR (odds ratio [OR]: 1.462, 95 % confidence interval [CI] 1.170-1.825, P = 0.001). The area under receiver operating characteristic curve (AUROC) of baseline anti-HBc level for predicting SR was 0.781 with the cut-off of 11.1 S/CO, with a sensitivity of 77.27 % and a specificity of 72.82 %. Our findings highlighted that baseline serum anti-HBc level is a promising indictor for predicting HBeAg seroconversion in HBeAg-positive CHB patients after antiviral treatment.
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http://dx.doi.org/10.1016/j.antiviral.2021.105146DOI Listing
July 2021

Does coffee, tea and caffeine consumption reduce the risk of incident breast cancer? A systematic review and network meta-analysis.

Public Health Nutr 2021 Jul 27:1-13. Epub 2021 Jul 27.

Department of Clinical Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenyang110016, People's Republic of China.

Objective: We aimed to evaluate the association between coffee and/or tea consumption and breast cancer (BC) risk among premenopausal and postmenopausal women and to conduct a network meta-analysis.

Design: Systematic review and network meta-analysis.

Setting: We conducted a systematic review of electronic publications in the last 30 years to identify case-control studies or prospective cohort studies that evaluated the effects of coffee and tea intake.

Results: Forty-five studies that included more than 3 323 288 participants were eligible for analysis. Network meta-analysis was performed to determine the effects of coffee and/or tea consumption on reducing BC risk in a dose-dependent manner and differences in coffee/tea type, menopause status, hormone receptor and the BMI in subgroup and meta-regression analyses. According to the first pairwise meta-analysis, low-dose coffee intake and high-dose tea intake may exhibit efficacy in preventing ER(estrogen receptor)- BC, particularly in postmenopausal women. Then, we performed another pairwise and network meta-analysis and determined that the recommended daily doses were 2-3 cups/d of coffee or ≥5 cups/d of tea, which contained a high concentration of caffeine, particularly in postmenopausal women.

Conclusions: Coffee and tea consumption is not associated with a reduction in the overall BC risk in postmenopausal women and is associated with a potentially lower risk of ER- BC. And the highest recommended dose is 2-3 cups of coffee/d or ≥5 cups of tea/d. They are potentially useful dietary protectants for preventing BC.
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http://dx.doi.org/10.1017/S1368980021000720DOI Listing
July 2021

Abundance and diversity of antibiotic resistance genes possibly released to ambient air by experiments in biology laboratories.

Sci Total Environ 2021 Jul 21;797:149147. Epub 2021 Jul 21.

Institute of Environmental Engineering, ETH Zurich, Zurich 8093, Switzerland; Advanced Analytical Technologies, Empa, Ueberlandstrasse 129, 8600 Dübendorf, Switzerland. Electronic address:

Antibiotic resistance genes (ARG) have been considered as a global emerging threat to public health systems. As special locations where both antibiotics and ARGs are directly used, biology laboratories are poorly studied but potential important emission sources where not only the environmental stress is strong but also obtaining resistance is much easier comparing to other well studied hot spots including farms, hospitals, wastewater treatment plants and landfills where antibiotics but not ARGs are used or discharged. Therefore, in this study, 11 Swiss biology laboratories working on different fields and located in the city center, suburb and rural area were studied to reveal the abundance and diversity of airborne ARGs in them and their surrounding areas with Colony-forming units (CFU) cultivation and quantitative Polymerase Chain Reaction (qPCR). Most biology laboratories did not discharge significant amounts or varieties of ARGs and cultivable bacteria via air. No correlation was found between the number of CFUs and the abundance of 16S rRNA, but two clusters of correlated airborne ARGs, the animal husbandry related cluster, and city and hospital related cluster were identified in this study. Although most biology laboratories may not be the emission sources of a wide variety of airborne ARGs, the ARGs in the animal husbandry related cluster which are abundant in the animal laboratories and aadA1 which is abundant in the laboratories working on other eukaryocytes need to be furtherly studied to make sure if they are potential health risks for the researchers.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149147DOI Listing
July 2021

Acute kidney injuries induced by thrombotic microangiopathy following severe hemorrhage in puerperants: a case series and literature review.

Am J Transl Res 2021 15;13(6):6182-6190. Epub 2021 Jun 15.

Department of Nephrology, China-Japan Friendship Hospital Beijing 100029, China.

Background: Acute kidney injury in puerperants is generally caused by acute tubular necrosis and occasionally by thrombotic microangiopathy (TMA) following post-partum hemorrhage. However, TMA leads to worse clinical outcomes and is rarely reported in the literature. Therefore, this study aimed to evaluate the pathological mechanism behind the development of TMA in puerperants to improve the diagnosis and treatment of this condition.

Methods: Three patients diagnosed with severe postpartum hemorrhage and TMA from 2014 to 2017 at a nephrology center were retrospectively investigated.

Results: All patients had severe hemorrhage during delivery with a mean blood loss, 4.0 L (range, 2.7-5.0 L). AKI developed rapidly in these patients and was treated with hemodialysis. Following treatment, the mean volume of packed red blood cells was 2.3 L (range, 1.2-3.6 L), and the mean volume of resuscitation fluid was 3.7 L (range, 3.5-4.0 L). All patients had renal biopsy specimens with typical TMA and ATN changes on light microscopy. Two patients required a hysterectomy while another two patients received respiratory support. Only one patient received plasma exchange. None of the patients had recovered normal kidney function by the final follow-up (26-61 months), with two patients having stage 3 chronic kidney disease, and one patient having an end-stage renal disease requiring maintenance hemodialysis.

Conclusion: Severe postpartum hemorrhage could lead to TMA, in addition to the common finding of ATN. Renal histology revealed that poor renal outcomes could be attributed to TMA coexisting with ATN. The potential mechanism was ischemia-reperfusion, which was followed by endothelial cell injury and activation of the alternative complement pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290764PMC
June 2021

[email protected] Nanoparticles for Photodynamic/Photothermal Combined Cancer Therapy with Single Near-Infrared Irradiation.

Int J Nanomedicine 2021 16;16:4863-4871. Epub 2021 Jul 16.

Laboratory of Veterinary Pathology and Nanopathology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.

Background: Phototherapy has significant potential as an effective treatment for cancer. However, the application of a multifunctional nanoplatform for photodynamic therapy (PDT) and photothermal therapy (PTT) at a single excitation wavelength remains a challenge.

Materials And Methods: The double emulsion solvent evaporation method was used to prepare toluidine [email protected] lactic-co-glycolic acid ([email protected]) nanoparticles (NPs). The biocompatibility of [email protected] NPs was evaluated, and a 660 nm luminescence was used as the light source. The photothermal effect, photothermal stability, and singlet oxygen yield of NPs in an aqueous solution verified the feasibility of NPs as a PTT/PDT synergistic therapy drug.

Results: [email protected] NPs were successfully prepared and characterized. In vitro experiments demonstrated that [email protected] NPs can cause massive necrosis of tumor cells and induce apoptosis through a photodynamic mechanism under 660 nm laser irradiation. The [email protected] NPs also achieved optimal tumor inhibition effect in vivo.

Conclusion: The [email protected] NPs prepared in this study were applied as a dual-mode phototherapeutic agent under single laser irradiation. Both in vitro and in vivo experiments demonstrated the good potential of PTT/PDT for tumor inhibitors.
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http://dx.doi.org/10.2147/IJN.S304713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291662PMC
July 2021

Unbalanced status and multidimensional influences of municipal solid waste management in Africa.

Chemosphere 2021 Oct 15;281:130884. Epub 2021 May 15.

Department of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.

The overall status of MSW management in Africa is evaluated systematically, in sight of life cycle indicators such as MSW generation daily per capita, collection and treatment methods. The relations of life cycle indictors with driving factors, including economic level, geographical location, urbanization and solid waste legislation process, are discussed. The results present that the major indicators had obvious regional differences, which can divide Africa into three levels: North Africa and South Africa > West Africa and Central Africa > East Africa. Besides, the disposal methods in North Africa, South Africa and West Africa have evolved from simple open dumping to sanitary landfill, composting and recycle treatment, while opening dumping is still rampant in East Africa. With a combination tool of Principal Component Analysis and Multiple Linear Regression, it was identified that economic growth, urbanization and geographical location are the most critical factors influencing the unbalanced statue of MSW management in Africa. By cluster analysis, unbalance status of MSW management in African countries can be integrated into three grades of MSW management level: good, fair and poor.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130884DOI Listing
October 2021

Percutaneous Vertebroplasty for C1 Osteolytic Lesions via Lateral Approach Under Fluoroscopic Guidance.

J Pain Res 2021 13;14:2121-2128. Epub 2021 Jul 13.

Department of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

Objective: To report our experience of treating painful C1 osteolytic lesions with cement augmentation via a lateral approach under fluoroscopic guidance.

Materials And Methods: Nine consecutive patients (eight men and one woman; mean age: 56.7±13.2 years) with osteolytic lesions of the atlas who underwent cementoplasty via a lateral approach were enrolled in this study. The technical success rate, operation time, and complications were recorded. Visual analogue scale (VAS) and Neck disability index (NDI) were used to evaluate the pain relief and neck function status pre-procedure (baseline) and post-procedure (at 3 days; after 1, 3, 6, 12, 18, and 24 months; and at the last follow-up).

Results: The procedure was technically successful without any complications in all patients. The mean procedure time was 44.3±7.8 min. There were three cases of bone cement leakage, but no clinical symptoms. The mean VAS score decreased from 6.7±1.0 before the procedure to 3.7 three days after the procedure. The mean NDI score decreased from 67.3±11.2 before the procedure to 39.3±13.7 three days after the procedure. The VAS and NDI scores decreased at each follow-up time point, and the difference was statistically significant compared with the scores before the procedure (P<0.01).

Conclusion: Fluoroscopy-guided lateral approach is a feasible, safe, and effective method for treatment of osteolytic lesions of the atlas and can relieve pain and stabilize the bone.
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http://dx.doi.org/10.2147/JPR.S318236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286128PMC
July 2021

New Aspects of Degradation in Silicone Rubber under UVA and UVB Irradiation: A Gas Chromatography-Mass Spectrometry Study.

Polymers (Basel) 2021 Jul 5;13(13). Epub 2021 Jul 5.

Hubei Nuclear-Solid Physics Key Laboratory and Department of Physics, Wuhan University, Wuhan 430072, China.

In this paper, gas chromatography-mass spectrometry (GC-MS) and positron annihilation lifetime spectroscopy (PALS) were used to probe the changes of oligomers and the polydimethylsiloxane (PDMS) network in silicone rubber, after different durations of UVA/UVB irradiation. At the early stage (<300 h) of UVA/UVB irradiation, the concentration of D4-D9 decreases. The o-Ps intensity of the extracted silicone rubber increases in the stage after UVB irradiation. These results indicate the crosslinking of oligomers into the PDMS network. After a long duration (>300 h) of UVB irradiation, D4 was generated and the lifetime of τ3 also increased, indicating the rupture of the Si-O bond in the PDMS network. These two aging processes were termed the post curing process and the chain session process. The new finding was that UVA could only induce the post curing process; UVB causes the rupture of the chemical bond in silicone rubber. Photons of UVB could break the C-H bond, and then trigger the backbiting decomposition of PDMS, breaking the Si-O bond, while the photons of UVA cannot. The fact that D4 was generated after UVB irradiation can be used to evaluate the UVB stability of silicone rubber in the future.
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http://dx.doi.org/10.3390/polym13132215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271979PMC
July 2021

Cardiovascular and cerebrovascular events in patients with intracerebral hemorrhage: Clinical characteristics and long-term predictors.

J Clin Neurosci 2021 Aug 7;90:118-123. Epub 2021 Jun 7.

Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. Electronic address:

Few studies have examined the long-term prognosis of Chinese patients with intracerebral hemorrhage (ICH). This study assessed the clinical characteristics and predictors of vascular events occurring within 5 years after ICH. We included consecutive patients diagnosed with first-ever ICH between June 2013 and December 2014. Based on follow-up data (collected until December 2019), we used multivariable logistic regression to examine the clinical characteristics and long-term predictors of vascular events (including recurrent ICH, ischemic stroke, and acute coronary syndrome) in patients who survived more than 30 days after ICH. Across the 307 patients in our analysis, the 5-year mortality rate was 28.01%. Within 5 years after ICH, major vascular events were observed in 62 patients (17.82%, 95% CI 13.78-21.82%). We observed high incidence of recurrent ICH (8.91%) and ischemic stroke (10.06%), but low incidence of acute coronary syndrome (1.15%). Most cases of recurrent ICH (80.65%) occurred within 3 years after ICH. Age ≥56 years and history of ischemic stroke or transient ischemic attack (TIA) were identified as predictors of cardiovascular and cerebrovascular events. ICH survivors are at high risk of both cardiovascular and cerebrovascular events, especially older patients (≥56 years) and those who experienced ischemic stroke or TIA prior to their first ICH. Recurrent ICH is more likely to occur within the first three years after first ICH than at later times. Clinicians should monitor patients closely for adverse events, particularly during the first three years after initial ICH.
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http://dx.doi.org/10.1016/j.jocn.2021.05.037DOI Listing
August 2021

Mesenchymal stem cells: ideal seeds for treating diseases.

Hum Cell 2021 Jul 16. Epub 2021 Jul 16.

School of Laboratory Medicine, The First Affiliated Hospital, Bengbu Medical College, Bengbu, China.

Mesenchymal stem cells (MSCs), a kind of multipotent stem cells with self-renewal ability and multi-differentiation ability, have become the "practical stem cells" for the treatment of diseases. MSCs have immunomodulatory properties and can be used to treat autoimmune diseases, such as systemic lupus erythematosus (SLE) and Crohn's disease. MSCs also can be used in cancer and aging. At present, many clinical experiments are using MSCs. MSCs can reduce the occurrence of inflammation and apoptosis of tissue cells, and promote the proliferation of endogenous tissue and organ cells, so as to achieve the effect of repairing tissue and organs. MSCs presumably also play an important role in Corona Virus Disease 2019 (COVID-19) infection.
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http://dx.doi.org/10.1007/s13577-021-00578-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284686PMC
July 2021

Pancreatoduodenectomy within 2 weeks after endoscopic retrograde cholangio-pancreatography increases the risk of organ/space surgical site infections: a 5-year retrospective cohort study in a high-volume centre.

Gland Surg 2021 Jun;10(6):1852-1864

Department of Geriatrics Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: Organ/space surgical site infections (OSSI) after pancreaticoduodenectomy (PD) are not rare events. The role of diagnosis and treatment for pancreatic and biliary diseases with an endoscopic retrograde cholangio-pancreatography (ERCP) procedure is currently controversial. However, the ERCP procedure might play a role in surgical outcomes after PD.

Methods: We conducted a retrospective cohort study for patients who underwent PD in the First Affiliated Hospital with the Nanjing Medical University from 1st September 2012 to 31st January 2018. The relationship between ERCP exposure and OSSI after PD was analyzed by univariate and forward stepwise multivariate logistic regression model.

Results: Of the 1,365 patients who underwent PD, 136 developed OSSI (10.0%). We found that ERCP exposure before PD (EEBPD) was significantly associated with an increased incidence rate of post-operative pancreas fistula (POPF) [24.2% (23/95) 14.9% (189/1,270), risk ratio (RR) =1.63, 95% confidence interval (CI), 1.11-2.38, P=0.015]. Hypertension, a higher level of preoperative low-density lipoprotein (LDL) and creatinine (Cr) were associated with elevated risks of post-operative OSSI [adjusted odds ratio (Adj-OR) (95% CI) were 1.59 (1.09-2.32), 1.70 (1.16-2.51), 1.99 (1.36-2.92)], whereas a preoperatively higher level of aspartate aminotransferase (AST) would decrease the risk [Adj-OR (95% CI), 0.62 (0.42-0.91)]. Remarkably, EEBPD would significantly increase and more than double the OSSI risk [Adj-OR (95% CI), 2.56 (1.46-4.47)] especially if it was within 14 days before surgery (Spearman =-0.698, P<0.001).

Conclusions: ERCP, as an independent risk factor, significantly increased the risk of post-operative OSSI after PD if it is performed within 14 days prior to surgery. Our findings would assist clinical decision-making, and improve OSSI control and prevention.
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http://dx.doi.org/10.21037/gs-20-826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258873PMC
June 2021

Seven air-core fibers with germanium-doped high-index rings supporting hundreds of OAM modes.

Opt Express 2021 Jun;29(13):19540-19550

In this paper, we propose and design a multi-orbital-angular-momentum multi-ring air-core fiber, which has seven high-index rings with each ring supporting 62 radially fundamental OAM modes across C and L bands (from 1530 nm to 1625 nm), i.e. 434 OAM modes in total. The designed fiber features >4×10 intra-ring modal indices difference for OAM modes with the same topological charge l in a ring across the C and L bands. Moreover, it can keep <-52 dB crosstalk between the OAM modes in the adjacent rings at 1550 nm, and <-24 dB crosstalk across C and L bands after 100-km fiber propagation. This kind of seven-air-core-ring fiber would be a robust candidate for transmitting efficient OAM modes and boosting the capacity of optical fiber communications systems.
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http://dx.doi.org/10.1364/OE.431314DOI Listing
June 2021

Analogue of electromagnetically induced transparency in a metal-dielectric bilayer terahertz metamaterial.

Opt Express 2021 Jul;29(14):21810-21819

We realize and numerically demonstrate the analogue of electromagnetically induced transparency (EIT) with a high-Q factor in a metal-dielectric bilayer terahertz metamaterial (MM) via bright-bright mode coupling and bright-dark mode coupling. The dielectric MM with silicon dimer rectangular-ring-resonator (Si-DRR) supports either a bright high-Q toroidal dipole resonance (TD) or a dark TD with infinite Q value, while plasmonic MM with metallic rectangular-ring-resonator (M-RR) supports a low-Q electric dipole resonance (ED). The results show that the near-field coupling between the dark TD and bright ED behaves just as that between the two bright modes, which is dependent on the Q factor of the TD resonance. Further, due to the greatly enhanced near-field coupling between the bright ED and dark TD, the coupling distance is significantly extended to about 1.9 times of the wavelength (in media), and robust EIT with large peak value over 0.9 and high Q-factor is achieved. The proposed bilayer MM provides a new EIT platform for design and applications in high-Q cavities, sensing, and slow-light based devices.
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http://dx.doi.org/10.1364/OE.428758DOI Listing
July 2021

Microencapsulation of nattokinase from fermentation by spray drying: Optimization, comprehensive score, and stability.

Food Sci Nutr 2021 Jul 8;9(7):3906-3916. Epub 2021 Jun 8.

College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing China.

Nattokinase from fermentation has recently gained more attention due to its beneficial effects on cardiovascular system. However, the instability of free nattokinase limits its application. The aim of the study was to develop a spray-drying microencapsulation process to obtain the nattokinase powder with high activity, high quality, and strong storage stability. Hence, the microencapsulation process of nattokinase from fermentation by spray drying was optimized. Experiments of single-factor and response surface methodology were used to assess the comprehensive scores and nattokinase activities. According to single-factor and response surface methodology results, optimum parameters of microencapsulation process of the nattokinase power by spray drying were 30% of mass ratio of wall materials, 139°C of air inlet temperature, 8 L/h of feed rate, and 80°C of outlet temperature. The final optimized result encompassed a comprehensive score of 96, nattokinase activity of 1,340 IU/ml, and moisture content of 4.1 ± 0.1%. In addition, the microencapsulated nattokinase power showed strong storage stability in the conditions of different temperatures and pH. After 30 days of storage, the nattokinase powder was still white or light yellow, with a special smell, no peculiar smell and paste taste, and no impurity. These results build the basis of further industrialization of the nattokinase powder from fermentation broth by spray drying.
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http://dx.doi.org/10.1002/fsn3.2378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269611PMC
July 2021

A causal inference framework for cancer cluster investigations using publicly available data.

J R Stat Soc Ser A Stat Soc 2020 Jun 25;183(3):1253-1272. Epub 2020 Apr 25.

Harvard T.H. Chan School of Public Health, Boston MA, USA.

Often, a community becomes alarmed when high rates of cancer are noticed, and residents suspect that the cancer cases could be caused by a known source of hazard. In response, the US Centers for Disease Control and Prevention recommend that departments of health perform a standardized incidence ratio (SIR) analysis to determine whether the observed cancer incidence is higher than expected. This approach has several limitations that are well documented in the existing literature. In this paper we propose a novel causal inference framework for cancer cluster investigations, rooted in the potential outcomes framework. Assuming that a source of hazard representing a potential cause of increased cancer rates in the community is identified a priori, we focus our approach on a causal inference estimand which we call the causal SIR (cSIR). The cSIR is a ratio defined as the expected cancer incidence in the exposed population divided by the expected cancer incidence for the same population under the (counterfactual) scenario of no exposure. To estimate the cSIR we need to overcome two main challenges: 1) identify unexposed populations that are as similar as possible to the exposed one to inform estimation of the expected cancer incidence under the counterfactual scenario of no exposure, and 2) publicly available data on cancer incidence for these unexposed populations are often available at a much higher level of spatial aggregation (e.g. county) than what is desired (e.g. census block group). We overcome the first challenge by relying on matching. We overcome the second challenge by building a Bayesian hierarchical model that borrows information from other sources to impute cancer incidence at the desired level of spatial aggregation. In simulations, our statistical approach was shown to provide dramatically improved results, i.e., less bias and better coverage, than the current approach to SIR analyses. We apply our proposed approach to investigate whether trichloroethylene vapor exposure has caused increased cancer incidence in Endicott, New York.
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http://dx.doi.org/10.1111/rssa.12567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276584PMC
June 2020

Attacks on Formation Control for Multiagent Systems.

IEEE Trans Cybern 2021 Jul 14;PP. Epub 2021 Jul 14.

Multiagent systems (MASs) are distributed systems with two or more intelligent agents. Formation control is a significant control technique of MASs. To date, formation control on MASs is widely used in various fields, such as robots, spacecrafts, satellites, and unmanned aerial/surface/underwater vehicles. However, there is a relatively small body of literature that is concerned with security problems of formation control on MASs in past years. Our research represents the first step toward developing security attacks of formation control on MASs. Our study aims to investigate potential security problems of formation control on a multirobot system for the first time. We propose two kinds of control-level attacks and each kind of attack includes several specific attack forms. Then, we discuss specific features of formation control on a classical multirobot system and utilize theoretical analyses to illustrate how cyberattacks can influence the physical movements of robots. The experimental results of the proposed attacks show that attacks can easily interrupt formation movements of a multirobot system and several carefully designed attacks even can cause irreversible loss.
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http://dx.doi.org/10.1109/TCYB.2021.3089375DOI Listing
July 2021

Major pathologic response assessment and clinical significance of metastatic lymph nodes after neoadjuvant therapy for non-small cell lung cancer.

Mod Pathol 2021 Jul 12. Epub 2021 Jul 12.

Department of Pathology, Peking University Cancer Hospital & Institute, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing, People's Republic of China.

For neoadjuvant therapy in patients with non-small cell lung cancer, the major pathologic response of primary tumors may be an assessable and reliable surrogate measure of survival. Few studies have examined the pathologic evaluation of metastatic lymph node responses and their prognostic significance. This retrospective study enrolled 336 patients with non-small cell lung cancer (squamous cell carcinoma, n = 216; adenocarcinoma, n = 120) treated with neoadjuvant therapy including chemotherapy (n = 316) and targeted therapy (adenocarcinoma, n = 20). The treatment response of the primary tumor and lymph node metastases (LNM) were pathologically assessed according to the multidisciplinary recommendations of the International Association for the Study of Lung Cancer. The relationship of overall survival (OS) and disease-free survival (DFS) with the responses of the primary tumor or LNM was analyzed. The optimal cutoff value of the residual viable tumor (%RVT) of the primary tumor was 12% for both OS (P < 0.001) and DFS (P < 0.001). The pathologic assessment identified LNM in 208 patients. The optimal %RVT cutoff value in LNM was 8% for both OS (P = 0.003) and DFS (P < 0.001). The Spearman's rank correlation coefficient between primary tumors and corresponding LNM was 0.487 for %RVT (P < 0.001), which indicated a positive correlation. On multivariable analysis, an RVT of the primary tumor ≤12% was an independent prognostic factor for improved OS (P = 0.024), whereas an RVT of LNM ≤ 8% was an independent prognostic factor for increased DFS (P = 0.018). Furthermore, in the neoadjuvant chemotherapy group, the optimal %RVT cutoff values for OS in patients with squamous cell carcinoma and adenocarcinoma in the primary tumor were 12% and 58%, respectively. Considering its convenience and operability in clinical application, a 10% threshold RVT value can be used for prognostic evaluation of LNM and primary tumors of squamous cell carcinoma histology; further studies are needed to confirm the optimal cutoff value for primary tumors of adenocarcinoma.
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http://dx.doi.org/10.1038/s41379-021-00871-1DOI Listing
July 2021

Thymocytes induce renal tubular epithelial cells to undergo the epithelial-to-mesenchymal transition.

Asian Pac J Allergy Immunol 2021 Jul 11. Epub 2021 Jul 11.

Department of Pathology, The First Hospital of Jilin University, Changchun, China.

Background: Renal tubulointerstitial fibrosis is known to occur as a result of epithelial cell transformation into myofibroblasts via the epithelial-to-mesenchymal transition (EMT) process. It has been reported that macrophages, regulatory T (Treg) cells, and gamma delta T (γδ T) cells can promote fibrosis via EMT in vivo.

Objective: Our study intended to detect whether thymocytes can induce renal tubular cells to undergo the EMT.

Methods: Rat thymocytes were activated by phytohemagglutinin and concanavalin A. The rat renal tubular epithelial cells (NRK-52E) were incubated in a conditioned medium harvested from activated thymocytes or co-cultured with freshly isolated thymocytes for 48 hours. Real-time reverse transcription-polymerase chain reaction, immunofluorescence, and western blotting analysis were used to test the expression of the epithelial and mesenchymal markers in NRK-52E cells. Scratch assay was designed to test the cell migration abilities of NRK-52E cells. Student's t test and oneway analysis of variance test were used for statistical analysis.

Results: The combined stimulation with phytohemagglutinin and concanavalin A activated the primary isolated rat thymocytes. After treatment with conditioned medium or freshly isolated thymocytes, the expression levels of cytokeratin 19 and E-cadherin were downregulated in NRK-52E cells, while the mRNA and protein expression levels of alpha-smooth muscle actin, desmin, and vimentin were upregulated (P < 0.05). We found that the cell migration abilities of the induced NRK-52E cells were significantly improved.

Conclusions: Both activated rat thymocytes (more percentage of CD8+ T cells) and freshly isolated thymocytes have promoting effects on the EMT of NRK-52E cells.
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http://dx.doi.org/10.12932/AP-210221-1075DOI Listing
July 2021

Advances in Pathophysiology of Triple-Negative Breast Cancer: The Potential of lncRNAs for Clinical Diagnosis, Treatment, and Prognostic Monitoring.

Mol Biotechnol 2021 Jul 10. Epub 2021 Jul 10.

School of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.

Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in several gene expression regulation processes, including epigenetic regulation, transcriptional regulation, post-transcriptional regulation, and translation regulation. It also plays a crucial role in the regulation of several characteristics of cancer biology, and the dysregulation of lncRNA expression in cancer may be part of the cause of cancer progression. Meanwhile, more and more studies are trying to determine the association between lncRNA expression and TNBC, as well as the functional role and molecular mechanism of the abnormally expressed lncRNA. Therefore, this review lists some abnormal lncRNAs in TNBC, further analyzes their molecular mechanisms and biological roles in the development of TNBC, and summarizes the potential of lncRNAs as biomarkers and therapeutic targets of TNBC, so as to provide ideas for clinical diagnosis, targeted therapy, and prognosis monitoring of TNBC.
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http://dx.doi.org/10.1007/s12033-021-00368-xDOI Listing
July 2021

Synthesis of sodium silicate using industrial by-products glauber's salt and microsilica: Effective reuse of the waste.

Waste Manag 2021 Jul 6;131:359-367. Epub 2021 Jul 6.

School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, PR China. Electronic address:

Large amounts of by-products, including glauber's salt (GS) and microsilica (MS), are accumulated from chlor-alkali industry and ferrosilicon industry development, which not only wastes precious resources, but also causes serious environmental problems. In order to recycle and reuse these industrial by-products, solid sodium silicate was synthesized using GS and MS as the main raw materials, and semi-coke (SC) as the reducing agent. The effects of melting parameters including GS/SC and MS/GS molar ratio, heating rate, temperature, reaction time, and SC particle size on the conversion efficiency and modulus of solid sodium silicate were investigated and optimized. Under optimal conditions, the maximum conversion efficiency of 94.91% was obtained with modulus of 2.5. Characterization analysis of the products indicated that the amorphous silicate sodium was successfully synthesized. Moreover, the reaction mechanism was investigated, which focused on the thermal behavior and phase transformation using thermo-gravimetric and differential scanning calorimetric (TG-DSC) and in-situ X-ray diffraction. Additionally, the causes and suppression measures of "glauber's salt water" (GSW) during the experiment were summarized. This work not only creates resource utilization of GS and MS, but also provides the foundation for the synthesis of sodium silicate with GS.
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http://dx.doi.org/10.1016/j.wasman.2021.06.026DOI Listing
July 2021

IRE1-mTOR-PERK Axis Coordinates Autophagy and ER Stress-Apoptosis Induced by P2X7-Mediated Ca Influx in Osteoarthritis.

Front Cell Dev Biol 2021 17;9:695041. Epub 2021 Jun 17.

Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, China.

Moderate-intensity exercise can help delay the development of osteoarthritis (OA). Previous studies have shown that the purinergic receptor P2X ligand gated ion channel 7 (P2X7) is involved in OA development and progression. To investigate the effect of exercise on P2X7 activation and downstream signaling in OA, we used the anterior cruciate ligament transection (ACLT)-induced OA rat model and primary chondrocyte culture system. Our experiments confirmed that treadmill exercise increased P2X7 expression and that this effect was more pronounced at the later time points. Furthermore, P2X7 activation induced endoplasmic reticulum (ER) stress and increased the expression levels of ER stress markers, such as 78 kDa glucose-regulated protein (GRP78), protein kinase R-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme-1 (IRE1), and activating transcription factor 6 (ATF6). At the early time points, IRE1 and PERK were activated, and mTOR was inhibited. At the later time points, mTOR was activated, mediating PERK to promote ER stress-apoptosis, whereas IRE1 and autophagy were inhibited. To confirm our observations , we treated primary chondrocytes with the P2X7 agonist benzoylbenzoyl-ATP (Bz-ATP). Our results confirmed that P2X7-mediated Ca influx activated IRE1-mediated autophagic flux and induced PERK-mediated ER stress-apoptosis. To further investigate the role of P2X7 in OA, we injected mTOR antagonist rapamycin or P2X7 antagonist A740003 into the knee joints of ACLT rats. Our results demonstrated that mTOR inhibition induced autophagy, decreased apoptosis, and reduced cartilage loss. However, injection of mTOR agonist MHY1485 or Bz-ATP had the opposite effect. In summary, our results indicated that during the early stages of moderate-intensity exercise, P2X7 was activated and autophagic flux was increased, delaying OA development. At the later stages, P2X7 became over-activated, and the number of apoptotic cells increased, promoting OA development. We propose that the IRE1-mTOR-PERK signaling axis was involved in the regulation of autophagy inhibition and the induction of apoptosis. Our findings provide novel insights into the positive and preventative effects of exercise on OA, suggesting that the intensity and duration of exercise play a critical role. We also demonstrated that on a molecular level, P2X7 and its downstream pathways could be potential therapeutic targets for OA.
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http://dx.doi.org/10.3389/fcell.2021.695041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248364PMC
June 2021

Graphene-Assisted Polymer Waveguide Optically Controlled Switch Using First-Order Mode.

Polymers (Basel) 2021 Jun 28;13(13). Epub 2021 Jun 28.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

All-optical devices have a great potential in optical communication systems. As a new material, graphene has attracted great attention in the field of optics due to its unique properties. We propose a graphene-assisted polymer optically controlled thermo-optic switch, based on the E mode, which can reduce the absorption loss of graphene. Graphene absorbs 980 nm pump light, and uses the heat generated by ohmic heating to switch on and off the signal light at 1550 nm. The simulation results show that, when the graphene is in the right position, we can obtain the power consumption of 9.5 mW, the propagation loss of 0.01 dB/cm, and the switching time of 127 μs (rise)/125 μs (fall). The switching time can be improved to 106 μs (rise) and 102 μs (fall) with silicon substrate. Compared with an all-fiber switch, our model has lower power consumption and lower propagation loss. The proposed switch is suitable for optically controlled fields with low loss and full polarization. Due to the low cost and easy integration of polymer materials, the device will play an important role in the fields of all-optical signal processing and silicon-based hybrid integrated photonic devices.
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http://dx.doi.org/10.3390/polym13132117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272245PMC
June 2021

A Synchronous Prediction Model Based on Multi-Channel CNN with Moving Window for Coal and Electricity Consumption in Cement Calcination Process.

Sensors (Basel) 2021 Jun 23;21(13). Epub 2021 Jun 23.

School of Electrical Engineering, Yanshan University, 438 Hebei Avenue, Qinhuangdao 066004, China.

The precision and reliability of the synchronous prediction of multi energy consumption indicators such as electricity and coal consumption are important for the production optimization of industrial processes (e.g., in the cement industry) due to the deficiency of the coupling relationship of the two indicators while forecasting separately. However, the time lags, coupling, and uncertainties of production variables lead to the difficulty of multi-indicator synchronous prediction. In this paper, a data driven forecast approach combining moving window and multi-channel convolutional neural networks (MWMC-CNN) was proposed to predict electricity and coal consumption synchronously, in which the moving window was designed to extract the time-varying delay feature of the time series data to overcome its impact on energy consumption prediction, and the multi-channel structure was designed to reduce the impact of the redundant parameters between weakly correlated variables of energy prediction. The experimental results implemented by the actual raw data of the cement plant demonstrate that the proposed MWMC-CNN structure has a better performance than without the combination structure of the moving window multi-channel with convolutional neural network.
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http://dx.doi.org/10.3390/s21134284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271547PMC
June 2021

Field Experiment Effect on Citrus Spider Mite of Venom from Jellyfish : The Potential Use of Jellyfish in Agriculture.

Toxins (Basel) 2021 06 10;13(6). Epub 2021 Jun 10.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.

Jellyfish are rich in resources and widely distributed along coastal areas. As a potential approach to respond to jellyfish blooms, the use of jellyfish-derived products is increasing. The citrus spider mite () is one of the key citrus pests, negatively impacting the quality and quantity of oranges. Due to the resistance and residue of chemical acaricides, it is important to seek natural substitutes that are environmentally friendly. The field efficacy of the venom from the jellyfish against was assayed in a citrus garden. The frozen tentacles were sonicated in different buffers to isolate the venom. The venom isolated by PBS buffer (10 mM, pH 6.0) had the strongest acaricidal activity of the four samples, and the corrected field efficacy 7 days after treatment was up to 95.21%. This study demonstrated that jellyfish has potential use in agriculture.
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http://dx.doi.org/10.3390/toxins13060411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229195PMC
June 2021

New insights on the effects of spinosad on the development of Helicoverpa armigera.

Ecotoxicol Environ Saf 2021 Sep 29;221:112452. Epub 2021 Jun 29.

State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. Electronic address:

Helicoverpa armigera (cotton bollworm) is one of the most destructive pests worldwide. Due to resistance to Bacillus thuringiensis and conventional insecticides, an effective management strategy to control this pest is urgently needed. Spinosad, a natural pesticide, is considered an alternative; however, the mechanism underlying the developmental effects of sublethal spinosad exposure remains elusive. In this study, the mechanism was examined using an insect model of H. armigera. Results confirmed that exposure to sublethal spinosad led to reduced larval wet weight, delayed larval developmental period, caused difficulty in molting, and deformed pupae. Further investigation demonstrated that exposure to sublethal spinosad caused a significant decrease in 20E titer and increase in JH titer, thereby leading to the discordance between 20E and JH titers, and consequently alteration in the expression levels of HR3 and Kr-h1. These results suggested that sublethal spinosad caused hormonal disorders in larvae, which directly affect insect development. Our study serves as a reference and basis for the toxicity evaluation of spinosad on molting and pupation in insect metamorphosis, which may contribute to identifying targets for effective control of cotton bollworm.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112452DOI Listing
September 2021
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