Publications by authors named "Yue Xu"

709 Publications

RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis.

Parasit Vectors 2022 Aug 4;15(1):279. Epub 2022 Aug 4.

School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

Background: Toxocara canis is a cosmopolitan parasite with a significant adverse impact on the health of humans and animals. The spleen is a major immune organ that plays essential roles in protecting the host against various infections. However, its role in T. canis infection has not received much attention.

Methods: We performed sequencing-based transcriptome profiling of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the spleen of Beagle puppies at 24 h post-infection (hpi), 96 hpi and 36 days post-infection (dpi). Deep sequencing of RNAs isolated from the spleen of six puppies (three infected and three control) at each time point after infection was conducted.

Results: Our analysis revealed 614 differentially expressed (DE) lncRNAs and 262 DEmRNAs at 24 hpi; 726 DElncRNAs and 878 DEmRNAs at 96 hpi; and 686 DElncRNAs and 504 DEmRNAs at 36 dpi. Of those, 35 DElncRNA transcripts and 11 DEmRNAs were detected at all three time points post-infection. Many DE genes were enriched in immune response, such as ifit1, ifit2 and rorc. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that some genes (e.g. prkx and tnfrsf11a) were involved in the T cell receptor signaling pathway, calcium signaling pathway, Ras signaling pathway and NF-κB signaling pathway.

Conclusions: The findings of this study show marked alterations in the expression profiles of spleen lncRNAs and mRNAs, with possible implications in the pathophysiology of toxocariasis.
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http://dx.doi.org/10.1186/s13071-022-05380-xDOI Listing
August 2022

Adapting parent-focused interventions for diverse caregivers of children with intellectual and developmental disabilities: Lessons learned during global crises.

J Policy Pract Intellect Disabil 2022 ;na:1-13

Department of Health Sciences Education, University of Illinois College of Medicine at Rockford, Rockford, Illinois, USA.

Parent-focused interventions have been designed to provide training and support to caregivers who are essential in achieving positive outcomes for children with intellectual and developmental disabilities (IDD). In 2020, significant crises, including the COVID-19 pandemic and continued racial tensions, profoundly impacted the livelihood of children with IDD and their families. Many ongoing efforts to address disparities among this population were halted temporarily and required further adaptations. Researchers adapted interventions and support to address the disparities impacting children with IDD and their families with limited guidance. We provide a descriptive case analysis of four parent-focused interventions that responded to the global crises to continue serving children with IDD and their families. The four distinct programs were based on applied behavior analysis and naturalistic, developmental-behavioral paradigms that were culturally adapted for families of young children with IDD from diverse cultural and socioeconomic backgrounds. We present the qualitative reports on the challenges and benefits that arose with adapting the four parent-focused interventions for telehealth implementation. We focused specifically on adaptations made in recruitment and retention, instrumentation and measurement, research staff training, and intervention delivery. We synthesize our experience with challenges and solutions in adapting parent-focused interventions for racially/ethnically and socioeconomically diverse children with IDD and their families. We conclude with recommendations for researchers and practitioners on methods for adapting parent-focused interventions to address the significant health disparities that impact racially, ethnically, and socioeconomically diverse children with IDD and their families.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340799PMC
January 2022

The Mechanisms of Ferroptosis and the Applications in Tumor Treatment: Enemies or Friends?

Front Mol Biosci 2022 15;9:938677. Epub 2022 Jul 15.

School of Laboratory Medicine and Biological Engineering, Hangzhou Medical College, Hangzhou, China.

Ferroptosis, as a newly discovered non-apoptotic cell death mode, is beginning to be explored in different cancer. The particularity of ferroptosis lies in the accumulation of iron dependence and lipid peroxides, and it is different from the classical cell death modes such as apoptosis and necrosis in terms of action mode, biochemical characteristics, and genetics. The mechanism of ferroptosis can be divided into many different pathways, so it is particularly important to identify the key sites of ferroptosis in the disease. Herein, based on ferroptosis, we analyze the main pathways in detail. More importantly, ferroptosis is linked to the development of different systems of the tumor, providing personalized plans for the examination, treatment, and prognosis of cancer patients. Although some mechanisms and side effects of ferroptosis still need to be studied, it is still a promising method for cancer treatment.
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http://dx.doi.org/10.3389/fmolb.2022.938677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334798PMC
July 2022

Nitric oxide-containing supramolecular polypeptide nanomedicine based on [2]biphenyl-extended-pillar[6]arenes for drug resistance reversal.

J Mater Chem B 2022 Jul 27. Epub 2022 Jul 27.

School of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, P. R. China.

A kind of supramolecular polypeptide nanomedicine (BPC/DOX-ICG) was constructed with an anionic water-soluble [2]biphenyl-extended-pillar[6]arene (AWBpP6), and pyridinium-terminal- and -nitrosothiol (SNO)-modified polypeptide (PPNC) host-guest interactions to co-deliver doxorubicin (DOX) and indocyanine green (ICG) for drug resistance reversal. Upon near-infrared (NIR) irradiation, the NO generation could down-regulate the P-glycoprotein (P-gp) expression level to reverse multidrug resistance (MDR). Subsequently, the resulting reverse MDR could sensitize the free DOX and assist photothermal therapy (PTT) to enhance the tumoricidal potential. This supramolecular polypeptide nanomedicine provides an effective strategy for the multimodal synergistic therapies of photothermal therapy, NO generation therapy, and chemotherapy (, PTT-NO-CT) to overcome MDR.
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http://dx.doi.org/10.1039/d2tb01127aDOI Listing
July 2022

Cannabidiol attenuates methamphetamine-induced conditioned place preference in male rats and viability in PC12 cells through the Sigma1R/AKT/GSK3β/CREB signaling pathway.

Am J Drug Alcohol Abuse 2022 Jul 26:1-14. Epub 2022 Jul 26.

School of Forensic Medicine, Kunming Medical University, Kunming, China.

Methamphetamine use is associated with several negative consequences, including neurotoxicity and greater probability of exhibiting a substance use disorder. Sigma1 receptor is involved in the neurobiological basis of several drug use disorders. Cannabidiol has received attention in the treatment of drug use disorders and neurotoxicity.

To investigate the effects of cannabidiol on methamphetamine-induced conditioned place preference (CPP) and the viability of PC12 cells.

Adult male rats (n = 70) underwent methamphetamine (2 mg/kg, IP) induced CPP, and were administered cannabidiol (10, 20, 40, or 80 mg/kg, IP) during the methamphetamine withdrawal period for five consecutive days. Methamphetamine (0.5 mg/kg) was then injected to reactivate CPP. Four brain regions (ventral tegmental area, nucleus accumbens, prefrontal cortex, and hippocampus) were extracted after the last test. PC12 cells were treated with cannabidiol, Sigma1R-siRNA, or BD1047 before methamphetamine exposure.

Administration of 20, 40, or 80 mg/kg cannabidiol facilitated CPP extinction (80 mg/kg, p < .001) and prevented CPP development (80 mg/kg, p < .0001). This was associated with changes in the expression of Sigma1R (ventral tegmental area, 80 mg/kg, p < .0001) in the four brain regions. Cannabidiol protected the PC12 cell's viability (10 μM, p = .0008) and inhibited the methamphetamine-induced activation of the AKT/GSK3β/CREB signaling pathway by mediating Sigma1R (10 μM, p < .0001).

Cannabidiol seems to inhibit the rewarding effects of methamphetamine and the effects of this drug on cell viability. Sigma1R should be given further consideration as a potential target for cannabidiol.
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http://dx.doi.org/10.1080/00952990.2022.2073450DOI Listing
July 2022

Effect of pretreatment with Phanerochaete chrysosporium on physicochemical properties and pyrolysis behaviors of corn stover.

Bioresour Technol 2022 Jul 22;361:127687. Epub 2022 Jul 22.

School of Life Science and Food Engineering, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huai'an 223003, China. Electronic address:

Fungal pretreatment can selectively degrade partial biomass components, which undoubtedly exerts a significant influence on biomass pyrolysis behavior. The corn stover was pretreated with Phanerochaete chrysosporium, and its influence on the physicochemical properties and pyrolysis behaviors of biomass together with the product characteristics were investigated. The Phanerochaete chrysosporium was more active to degrade hemicellulose and lignin. The hemicellulose and lignin contents in corn stover were decreased by 35.14 % and 31.80 %, respectively, after five weeks pretreatment, compared to the untreated sample. The reaction activation energy decreased from 52.89 kJ·mol for the untreated sample to 40.88 kJ·mol for the sample pretreated for five weeks. The Phanerochaete chrysosporium pretreatment was beneficial to the biochar production but exerted an unfavorable effect on the texture structure. The Phanerochaete chrysosporium also had an obvious influence on the bio-oil compositions. This study can provide a scientific reference for the application of biological pretreatment for biomass pyrolysis technology.
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http://dx.doi.org/10.1016/j.biortech.2022.127687DOI Listing
July 2022

Efficient Robust Yield Method for Preparing Bacterial Ghosts by Phage ID52 Lysis Protein E.

Bioengineering (Basel) 2022 Jul 7;9(7). Epub 2022 Jul 7.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.

Bacterial ghosts (BGs) are nonliving empty bacterial shells without cytoplasm retaining original morphology and identical antigenicity of natural bacteria, making them high potential and promising vaccine candidates and delivery vehicles. However, the low yield of commonly used BGs preparation methods limits its mass production and widely application. In order to improve BGs production, phage ID52 lysis protein E was introduced to generating BGs for the first time. Above all, we compared the lysis activity of lysis protein of phage φX174 and phage ID52 as well as the effects of promoters on the lysis activity of ID52-E, which shown that the lysis activity and BGs formation rate of protein ID52-E was significantly higher than protein φX174-E. Further, the lysis activity of ID52-E was significantly improved under the control of L-arabinose inducible promoter which initial induction OD reached as high as 2.0. The applicability of lysis protein ID52-E induced by L-arabinose was proved by preparing probiotic Nissle 1917 BGs and pathogenic BGs in mass production. This paper introduced a novel and highly efficient method for BGs preparation depending on recombinant expression of phage ID52-E under eco-friendly and reasonable price inducer L-arabinose.
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http://dx.doi.org/10.3390/bioengineering9070300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311611PMC
July 2022

MiR-208b/miR-21 Promotes the Progression of Cardiac Fibrosis Through the Activation of the TGF-β1/Smad-3 Signaling Pathway: An and Study.

Front Cardiovasc Med 2022 5;9:924629. Epub 2022 Jul 5.

Department of Cardiology, Ninth Hospital of Xi'an, Xi'an, China.

Background: Regulatory molecule microRNAs (miRNAs) have been implicated in myocardial fibrosis. However, the specific mechanism by which they lead to myocardial fibrosis remains unclear. This study aimed to explore the roles of miR-208b, miR-21 and transforming growth factor-β1 (TGF-β1)/Smad-3 signaling pathway components in cardiac fibrosis development.

Materials And Methods: Thirty-six consecutive acute myocardial infarction (AMI) patients were included in this study. Plasma was collected on admission and at 24 h, 48 h and 6 d. The levels of plasma miR-208b, miR-21, TGF-β1, and Smad-3 were measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and cardiac calcium protein T (cTnT) and creatine kinase isoenzyme (CK-MB) were detected by electrochemiluminescence analysis. H9C2 cells were exposed to hypoxia and divided into 4 groups (hypoxia treatment for 6 h, 24 h, 48 h, and 72 h). These stimulated cells were then transfected with miRNA inhibitors and mimics for gene overexpression and inhibition. RT-qPCR was used to detect the expression of miR-208b, miR-21, TGF-β1, and Smad-3, and western blot analysis was used to detect TGF-β1 and Smad-3 protein expression.

Results: The plasma analysis showed cTnT and CK-MB expression peaked at 24 h after symptom onset; miR-208b, miR-21, TGF-β1, and Smad-3 levels showed no peak and increased gradually with time. Cell experiments revealed that miR-208b and TGF-β1 were upregulated along with increased hypoxia exposure; miR-21 expression peaked at 24 h and 72 h, with the highest peak at 72 h, and Smad-3 expression peaked at 6 h and 72 h, with the highest peak at 72 h. miR-208b and miR-21 expressions were positively correlated with TGF-β/Smad-3 expression. TGF-β1/Smad-3 mRNA and protein levels were elevated in the miR-208b and miR-21 overexpression groups and reduced in the miR-208b and miR-21 inhibition groups.

Conclusion: MiR-208b and miR-21 promote cardiac fibrosis progression through TGF-β1/Smad-3 signaling pathway activation.
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http://dx.doi.org/10.3389/fcvm.2022.924629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294285PMC
July 2022

Estrogen Mediates an Atherosclerotic-Protective Action Estrogen Receptor Alpha/SREBP-1 Signaling.

Front Cardiovasc Med 2022 5;9:895916. Epub 2022 Jul 5.

Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, China.

Menopause is associated with dyslipidemia and an increased risk of cardiovascular disease, the underlying mechanism of dyslipidemia is attributed to an insufficiency of estrogen. In this study, we find that estrogen mediates an atherosclerotic-protective action estrogen receptor alpha/SREBP-1 signaling. Increased lipid accumulation and low-density lipoprotein (LDL)-uptake in HepG2 cells and THP-1 macrophages were induced by treatment of mixed hyperlipidemic serum from postmenopausal women; 17β-estradiol [estrogen (E2)] (10 nM) administration significantly improved hyperlipidemic profiles, relieved fatty-liver damage and attenuated the plaque area in the heart chamber of high-fat diet (HFD)-fed ovariectomized (OVX) Apo mice. Expression of sterol regulatory element-binding protein (SREBP)-1 mRNA of circulating leukocytes in postmenopausal women was strongly correlated to the serum E2 level. Exploration of data from the Gene Expression Profiling Interactive Analysis (GEPIA) database revealed that expression of SREBP-1 protein correlated to expression of estrogen receptor (ESR)α protein in the liver, blood and in normal tissue. Genetic overexpression/inhibition of ESRα resulted in increased/decreased SREBP-1 expression as well as attenuated/deteriorated lipid deposition . An inhibitor of the protein kinase B/mammalian target of rapamycin (AKT/mTOR) pathway, AZD8055, abolished ESRα-induced SREBP-1 expression in HepG2 cells. Moreover, E2 and statin co-treatment significantly reduced lipid accumulation and hindered the progression of atherosclerosis and fatty-liver damage in OVX Apo mice. Collectively, our results suggest that estrogen could exerted its atherosclerotic-protective action ESRα/SREBP-1 signaling. E2 might enhance the cellular sensitivity of statins and could be used as a novel therapeutic strategy against atherosclerotic disorders in postmenopausal women.
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http://dx.doi.org/10.3389/fcvm.2022.895916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294214PMC
July 2022

Associations Between Asthma and Polycystic Ovary Syndrome: Current Perspectives.

Front Endocrinol (Lausanne) 2022 5;13:936948. Epub 2022 Jul 5.

Obstetrics & Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China.

A potential correlation between polycystic ovary syndrome (PCOS) and asthma, used to be identified as diseases originating from two independent systems, has been supported by increasing evidence. From an epidemiological perspective, mounting studies have confirmed that women suffering from PCOS exhibit increased susceptibility to asthma. Meanwhile, PCOS and asthma seem to share several mutual pathological conditions, such as metabolic disorders, hormonal fluctuation, proinflammatory state, etc. Here, we further elucidate the correlation between asthma and PCOS by focusing on the internal common pathophysiology and adverse influences on women's health. Understanding the internal connection between PCOS and asthma may shed light on developing new prevention and control strategies to fight against these conditions.
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http://dx.doi.org/10.3389/fendo.2022.936948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294161PMC
July 2022

Cell nucleus localization and high anticancer activity of quinoline-benzopyran rhodium(III) metal complexes as therapeutic and fluorescence imaging agents.

Dalton Trans 2022 Jul 21. Epub 2022 Jul 21.

Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, P R China.

Four novel rhodium(III) complexes, [Rh(QB1)Cl(DMSO)] (RhN1), [Rh(QB2)Cl(CHOH)]·CHOH (RhN2), [Rh(QB3)Cl(CHOH)]·CHOH (RhS), and [Rh(QB4)Cl(DMSO)] (RhQ), bearing quinoline-benzopyran ligands (QB1-QB4) were synthesized and used to develop highly anticancer therapeutic and fluorescence imaging agents. Compared with the QB1-QB4 ligands (IC > 89.2 ± 1.7 μM for A549/DDP), RhN1, RhN2, RhS and RhQ exhibit selective cytotoxicity against lung carcinoma cisplatin-resistant A549/DDP (A549CDDP) cancer cells, with IC values in the range of 0.08-2.7 μM. The fluorescent imaging agent RhQ with the more extended planar QB4 ligand exhibited high anticancer activity in A549CDDP cells and was found in the cell nucleus fraction, whereas RhS had no fluorescence properties. RhQ and RhS may trigger cell apoptosis by causing DNA damage and initiating the mitochondrial dysfunction pathway. Furthermore, RhQ has a higher antitumor efficacy ( 55.3%) than RhS (46.4%) and cisplatin (CDDP, 33.1%), and RhQ demonstrated significantly lower toxicity than CDDP, making it a promising Rh(III)-based anticancer therapeutic and fluorescence imaging agent.
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http://dx.doi.org/10.1039/d2dt01929aDOI Listing
July 2022

Glycolysis in Innate Immune Cells Contributes to Autoimmunity.

Front Immunol 2022 1;13:920029. Epub 2022 Jul 1.

Department of Rheumatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Autoimmune diseases (AIDs) refer to connective tissue inflammation caused by aberrant autoantibodies resulting from dysfunctional immune surveillance. Most of the current treatments for AIDs use non-selective immunosuppressive agents. Although these therapies successfully control the disease process, patients experience significant side effects, particularly an increased risk of infection. There is a great need to study the pathogenesis of AIDs to facilitate the development of selective inhibitors for inflammatory signaling to overcome the limitations of traditional therapies. Immune cells alter their predominant metabolic profile from mitochondrial respiration to glycolysis in AIDs. This metabolic reprogramming, known to occur in adaptive immune cells, i.e., B and T lymphocytes, is critical to the pathogenesis of connective tissue inflammation. At the cellular level, this metabolic switch involves multiple signaling molecules, including serine-threonine protein kinase, mammalian target of rapamycin, and phosphoinositide 3-kinase. Although glycolysis is less efficient than mitochondrial respiration in terms of ATP production, immune cells can promote disease progression by enhancing glycolysis to satisfy cellular functions. Recent studies have shown that active glycolytic metabolism may also account for the cellular physiology of innate immune cells in AIDs. However, the mechanism by which glycolysis affects innate immunity and participates in the pathogenesis of AIDs remains to be elucidated. Therefore, we reviewed the molecular mechanisms, including key enzymes, signaling pathways, and inflammatory factors, that could explain the relationship between glycolysis and the pro-inflammatory phenotype of innate immune cells such as neutrophils, macrophages, and dendritic cells. Additionally, we summarize the impact of glycolysis on the pathophysiological processes of AIDs, including systemic lupus erythematosus, rheumatoid arthritis, vasculitis, and ankylosing spondylitis, and discuss potential therapeutic targets. The discovery that immune cell metabolism characterized by glycolysis may regulate inflammation broadens the avenues for treating AIDs by modulating immune cell metabolism.
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http://dx.doi.org/10.3389/fimmu.2022.920029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284233PMC
July 2022

Assessment of reporting completeness in acupuncture studies on patients with functional constipation using the STRICTA guidelines.

Complement Ther Med 2022 Jul 11;70:102849. Epub 2022 Jul 11.

Hospital of Chengdu University of Traditional Chinese Medicine, No. 39 Shi-er-qiao Road, Chengdu 610072, Sichuan Province, PR China. Electronic address:

Objective: To evaluate the completeness of reporting of acupuncture interventions in trials for functional constipation (FC) following the STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) guidelines.

Methods: We searched eight databases for all published trials, including clinical trials, pilot/feasibility studies, observational studies, and case studies, for acupuncture in patients with FC up to June 31, 2021. The completeness of reporting was evaluated using the STRICTA guidelines.

Results: Finally, 99 studies were included and analysed based on the latest STRICTA guidelines. Out of the 17 analysed STRICTA sub-items, only five were found to be appropriately reported in more than 90% of the trials, while five were completely reported in less than 30%.

Conclusions: The reporting completeness of acupuncture trials for FC in accordance with STRICTA guidelines is moderate, with poor guideline adherence for several items. Clinical trial reports should be further improved in accordance with STRICTA guidelines to enhance the completeness of evidence. There is also a need to explore the underlying reasons as to why the authors did not report these items and to develop strategies for improving guideline compliance.
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http://dx.doi.org/10.1016/j.ctim.2022.102849DOI Listing
July 2022

Construction of the Evaluation Index System for Nurse Deployment Pertaining to the Disaster Rescue.

Contrast Media Mol Imaging 2022 23;2022:2925689. Epub 2022 Jun 23.

The Health Service Training Center, Chinese PLA General Hospital, Beijing 100853, China.

Based on the Delphi method, the analytic hierarchy process, and the entropy method, this paper constructs the evaluation index system for nurse deployment pertaining to the disaster rescue in military hospitals to furnish the reference evidence for scientific deployment of nursing staff, thereby promoting the rescue supportability. This paper establishes the expert consultation form of the evaluation index system for nurse deployment pertaining to the disaster rescue in military hospitals through expert interviews, group discussions, and so on. The Delphi method is applied to enquire 20 military experts in different professional fields two times, and the evaluation index system is finally determined. The weights of evaluation indexes of disaster rescue nurses are determined by the analytic hierarchy process and entropy method. The construction of the evaluation index system for the deployment of disaster relief nurses in military hospitals through Delphi method, analytic hierarchy process, and entropy method provides a reference method for rational allocation of nurses and points out the key points of hospital training. In addition, this paper provides a reference for the assessment and selection of nurses related to disaster relief in military hospitals and lays a foundation for the construction of subsequent evaluation models, which is of great significance for improving the level of nursing teams.
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http://dx.doi.org/10.1155/2022/2925689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246577PMC
July 2022

Novel roles of LSECtin in gastric cancer cell adhesion, migration, invasion, and lymphatic metastasis.

Cell Death Dis 2022 Jul 11;13(7):593. Epub 2022 Jul 11.

Department of General Surgery, The Second Hospital of Dalian Medical University, 116023, Dalian, China.

Liver and lymph node sinusoidal endothelial cell C-type lectin (LSECtin) plays an important regulatory role in a variety of diseases, including tumors. However, the underlying mechanism of LSECtin in gastric cancer (GC) remains largely unknown. In our research, LSECtin promoted the adhesion and invasion of GC cells, and was involved in lymphatic metastasis of GC cells. Mechanistically, LSECtin promoted the adhesion, proliferation and migration of GC cells by downregulating STAT1 expression. The circular RNA circFBXL4, which is regulated by LSECtin, sponges the microRNA miR-146a-5p to regulate STAT1 expression. The promotion of GC cell proliferation, migration and invasion mediated by LSECtin was largely inhibited by circFBXL4 overexpression or miR-146a-5p silencing. Moreover, in its role as a transcription factor, STAT1 modulated the expression of FN1 and CHD4. In conclusion, LSECtin might be involved in the lymphatic metastasis of GC by upregulating the expression of FN1 and CHD4 via the circFBXL4/miR-146a-5p/STAT1 axis, possibly indicating a newly discovered pathogenic mechanism.
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http://dx.doi.org/10.1038/s41419-022-05026-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276708PMC
July 2022

Identification of CFHR4 as a Potential Prognosis Biomarker Associated With lmmune Infiltrates in Hepatocellular Carcinoma.

Front Immunol 2022 22;13:892750. Epub 2022 Jun 22.

Department of Minimal Invasive Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Complement factor H-related 4 (CFHR4) is a protein-coding gene that plays an essential role in multiple diseases. However, the prognostic value of CFHR4 in hepatocellular carcinoma (HCC) is unknown.

Methods: Using multiple databases, we investigated CFHR4 expression levels in HCC and multiple cancers. The relationship between CFHR4 expression levels and clinicopathological variables was further analyzed. Various potential biological functions and regulatory pathways of CFHR4 in HCC were identified by performing a Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Set Enrichment Analysis (GSEA). Single-sample gene set enrichment analysis (ssGSEA) was performed to confirm the correlation between CFHR4 expression and immune cell infiltration. The correlations between CFHR4 expression levels in HCC and N6-methyladenosine (m6A) modifications and the competing endogenous RNA (ceRNA) regulatory networks were confirmed in TCGA cohort.

Results: CFHR4 expression levels were significantly decreased in HCC tissues. Low CFHR4 expression in HCC tissues was significantly correlated with the patients' sex, race, age, TNM stage, pathological stage, tumor status, residual tumor, histologic grade and alpha fetal protein (AFP) level. GO and KEGG analyses revealed that differentially expressed genes related to CFHR4 may be involved in the synaptic membrane, transmembrane transporter complex, gated channel activity, chemical carcinogenesis, retinol metabolism, calcium signaling pathway, PPAR signaling pathway, insulin and gastric acid secretion. GSEA revealed that the FCGR-activated reaction, PLK1 pathway, ATR pathway, MCM pathway, cascade reactions of PI3K and FGFR1, reactant-mediated MAPK activation and FOXM1 pathway were significantly enriched in HCC with low CFHR4 expression. Moreover, CFHR4 expression was inversely correlated the levels of infiltrating Th2 cells, NK CD56bright cells and Tfh cells. In contrast, we observed positive correlations with the levels of infiltrating DCs, neutrophils, Th17 cells and mast cells. CFHR4 expression showed a strong correlation with various immunomarker groups in HCC. In addition, high CFHR4 expression significantly prolonged the overall survival (OS), disease-specific survival (DSS) and progression-free interval (PFI). We observed a substantial correlation between the expression of CFHR4 and multiple N6-methyladenosine genes in HCC and constructed potential CFHR4-related ceRNA regulatory networks.

Conclusions: CFHR4 might be a potential therapeutic target for improving the HCC prognosis and is closely related to immune cell infiltration.
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http://dx.doi.org/10.3389/fimmu.2022.892750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257081PMC
July 2022

Differences in actionable genomic alterations between brain metastases and non‑brain metastases in patients with non‑small cell lung cancer.

Int J Oncol 2022 09 7;61(3). Epub 2022 Jul 7.

Department of Pathology, Affiliated 3201 Hospital of Xi'an Jiaotong University, Shaanxi, Hanzhong 723000, P.R. China.

Brain metastases (BM) have been closely associated with increased morbidity and poor survival outcomes in patients with non‑small cell lung cancer (NSCLC). Excluding risk factors in histological subtypes, genomic alterations, including epidermal growth factor receptor mutations and anaplastic lymphoma kinase () rearrangements have been also regarded as greater risk factors for BM in the aspect of molecular subtypes. In the present study, 69 tumor tissues and 51 peripheral blood samples from patients with NSCLC were analyzed using a hybridization capture‑based next‑generation sequencing (NGS) panel, including 95 known cancer genes. Among the 90 patients with stage IV NSCLC, 26 cases suffered from BM and 64 cases did not. In total, 174 somatic mutations in 35 mutated genes were identified, and 12 of these genes were concurrently present in the BM group and the non‑BM group. Importantly, five mutated genes including , cytidine deaminase (), SMAD family member 4 (), superoxide dismutase 2 () and Von Hippel‑Lindau tumor suppressor () genes were uniquely detected in the BM group, and they were enriched in the Hippo signaling pathway, pyrimidine metabolism and pantothenate and co‑enzyme A (CoA) biosynthesis, as demonstrated using Kyoto Encyclopedia of Genes and Genomes enrichment analysis. RNA polymerase II transcription regulator complex and promyelocytic leukemia nuclear body were the top functional categories according to the Gene Ontology enrichment analysis in the BM group and non‑BM group, respectively. Furthermore, 43.33% (13/30) of mutated genes were detected by both tumor tissue deoxyribonucleic acid (DNA) and plasma‑derived circulating tumor DNA (ctDNA) in the non‑BM group, while this percentage was only limited to 29.41% (5/17) in the BM group. To summarize, significant differences in somatic mutations, somatic interactions, key signaling pathways, functional biological information, and clinical actionability for the therapy of targeted agents were founded between the BM group and the non‑BM group, and ctDNA analysis may by applied as a more credible alternative for genomic profiling in patients with advanced NSCLC without BM, due to its higher consistency for genomic profiling between ctDNA analysis and tissue DNA analysis.
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http://dx.doi.org/10.3892/ijo.2022.5390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291252PMC
September 2022

Dental plaque-inspired versatile nanosystem for caries prevention and tooth restoration.

Bioact Mater 2023 Feb 21;20:418-433. Epub 2022 Jun 21.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque. It remains challenges for current practice to specifically identify, intervene and interrupt the development of caries while restoring defects. In this study, inspired by natural dental plaque, a stimuli-responsive multidrug delivery system ([email protected]) has been developed to prevent tooth decay and promote enamel restoration. Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond, followed by conjugation with salivary-acquired peptide (SAP) to endow the nanoparticle with strong adhesion to tooth enamel. The constructed [email protected] specifically adheres to tooth, identifies cariogenic conditions and intelligently releases drugs at acidic pH, thereby providing antibacterial adhesion and cariogenic biofilm resistance, and restoring the microarchitecture and mechanical properties of demineralized teeth. Topical treatment with [email protected] effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues. These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.
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http://dx.doi.org/10.1016/j.bioactmat.2022.06.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233191PMC
February 2023

Iodine pentoxide-mediated oxidative selenation and seleno/thiocyanation of electron-rich arenes.

Org Biomol Chem 2022 Jul 13;20(27):5463-5469. Epub 2022 Jul 13.

School of Pharmacy and Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226019, People's Republic of China.

A simple and efficient method for the regioselective selenation of electron-rich arenes by employing non-metal inorganic iodine pentoxide (IO) as a reaction promoter under ambient conditions has been developed. The present protocol showed broad functional group tolerance and easy-to-operate and time-economical features. Additionally, this protocol also allows access to 3-seleno and 3-thiocyanoindoles by the use of readily available selenocyanate and thiocyanate salts. A mechanistic study indicated that the transformation operated through selenenyl iodide-induced electrophilic substitution processes.
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http://dx.doi.org/10.1039/d2ob00892kDOI Listing
July 2022

Linking the Fasting Blood Glucose Level to Short-Term-Exposed Particulate Constituents and Pollution Sources: Results from a Multicenter Cross-Sectional Study in China.

Environ Sci Technol 2022 07 30;56(14):10172-10182. Epub 2022 Jun 30.

China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.

Ambient PM (fine particulate matter with aerodynamic diameters ≤ 2.5 μm) is thought to be associated with the development of diabetes, but few studies traced the effects of PM components and pollution sources on the change in the fasting blood glucose (FBG). In the present study, we assessed the associations of PM constituents and their sources with the FBG in a general Chinese population aged over 40 years. Exposure to PM was positively associated with the FBG level, and each interquartile range (IQR) increase in a lag period of 30 days (18.4 μg/m) showed the strongest association with an elevated FBG of 0.16 mmol/L (95% confidence interval: 0.04, 0.28). Among various constituents, increases in exposed elemental carbon, organic matter, arsenic, and heavy metals such as silver, cadmium, lead, and zinc were associated with higher FBG, whereas barium and chromium were associated with lower FBG levels. The elevated FBG level was closely associated with the PM from coal combustion, industrial sources, and vehicle emissions, while the association with secondary sources was statistically insignificant. Improving air quality by tracing back to the pollution sources would help to develop well-directed policies to protect human health.
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http://dx.doi.org/10.1021/acs.est.1c08860DOI Listing
July 2022

Novel compound heterozygous SUCLG1 variants may contribute to mitochondria DNA depletion syndrome-9.

Mol Genet Genomic Med 2022 Jun 28:e2010. Epub 2022 Jun 28.

Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Background: Succinate-CoA ligase/synthetase (SCS) deficiency is responsible for encephalomyopathy with mitochondrial DNA depletion and mild methylmalonic aciduria. Variants in SUCLG1, the nuclear gene encoding the alpha subunit of the SCS enzyme playing a pivotal role in maintaining mtDNA integrity and stability, are associated with mitochondrial DNA depletion syndrome 9 (MTDPS9).

Methods: In this study, we reported an infant with clinical features of MTDPS9 from China. Whole exome sequencing (WES) was used to identify the genetic cause. Bioinformatic analysis and mtDNA level detection were performed to assess pathogenicity.

Results: The proband manifested with hypotonia, lactic acidosis, mild methylmalonic aciduria, hearing loss and psychomotor retardation. WES identified new compound heterozygous SUCLG1 variants of c.601A>G (p.R201G) in exon 6 and c.871G>C (p.A291P) in exon 8. Computational analysis predicted that these missense variants might alter structure stability and mitochondrial translocation of SUCLG1. qRT-PCR showed 68% depletion of mtDNA content in proband as compared to controls.

Conclusion: Novel compound heterozygous variants c.601A>G (p.R201G) and c.871G>C (p.A291P) in SUCLG1 may cause MTDPS9 in this family. Our finding should be helpful for molecular diagnosis, genetic counseling and clinical management of SCS deficiency disorders.
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http://dx.doi.org/10.1002/mgg3.2010DOI Listing
June 2022

Methylated as a triage marker for occult cervical cancer and advanced cervical intraepithelial neoplasia.

Future Oncol 2022 Jul 27;18(23):2583-2592. Epub 2022 Jun 27.

Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

To explore the appropriate triage methods for women infected with high-risk human papillomavirus (hrHPV). A total of 424 out of 872 hrHPV-infected women were divided into cervicitis (n = 123), cervical intraepithelial neoplasia grade 1 (CIN1; n = 89), CIN2 (n = 72), CIN3 (n = 87) and cervical cancer (n = 53) groups. The sensitivity/specificity of , and liquid-based cytology (LBC) for hrHPV-infected women with transformation zone 3 CIN3+ was 83.9/93.1, 77.4/90.6 and 80.6/58.5%, respectively. The / test had a higher specificity than LBC (p < 0.001) and similar sensitivity to that observed for LBC (p > 0.05). / improved the positive predictive value of CIN3+ (64.7/60.0%) in low-grade LBC (negative predictive value: 91.7/88.7%). was superior to and LBC tests in detecting CIN3+ in hrHPV-infected women.
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http://dx.doi.org/10.2217/fon-2021-1625DOI Listing
July 2022

Controllable synthesis of gold nanoparticle dimers site-selective growth.

Chem Commun (Camb) 2022 Jul 14;58(57):7932-7935. Epub 2022 Jul 14.

Department of Environmental Science and Engineering, University of Science and Technology of China, 230026 Hefei, China.

Random aggregation of nanoparticles follows step-growth kinetics, and thus the synthesis of large nanoparticle dimers is still a challenge. Here, we report a site-selective growth route to producing gold nanoparticle dimers in a high yield of up to 71%. The polymer contraction exposed two tips of gold nanorods, which provided active sites for further growth, setting the ability for a high yield of large dimers. Methods to fine tune the reaction rate allowed us to control the spacing between nanoparticles, giving a good SERS performance. Our method was shown to make up for the shortcomings of the existing synthetic method, and to achieve the synthesis of large dimers in solution.
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http://dx.doi.org/10.1039/d2cc00801gDOI Listing
July 2022

Predicting MGMT Promoter Methylation in Diffuse Gliomas Using Deep Learning with Radiomics.

J Clin Med 2022 Jun 15;11(12). Epub 2022 Jun 15.

Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.

This study aimed to investigate the feasibility of predicting oxygen 6-methylguanine-DNA methyltransferase (MGMT) promoter methylation in diffuse gliomas by developing a deep learning approach using MRI radiomics. A total of 111 patients with diffuse gliomas participated in the retrospective study (56 patients with MGMT promoter methylation and 55 patients with MGMT promoter unmethylation). The radiomics features of the two regions of interest (ROI) (the whole tumor area and the tumor core area) for four sequences, including T1 weighted image (T1WI), T2 weighted image (T2WI), apparent diffusion coefficient (ADC) maps, and T1 contrast-enhanced (T1CE) MR images were extracted and jointly fed into the residual network. Then the deep learning method was developed and evaluated with a five-fold cross-validation, where in each fold, the dataset was randomly divided into training (80%) and validation (20%) cohorts. We compared the performance of all models using area under the curve (AUC) and average accuracy of validation cohorts and calculated the 10 most important features of the best model via a class activation map. Based on the ROI of the whole tumor, the predictive capacity of the T1CE and ADC model achieved the highest AUC value of 0.85. Based on the ROI of the tumor core, the T1CE and ADC model achieved the highest AUC value of 0.90. After comparison, the T1CE combined with the ADC model based on the ROI of the tumor core exhibited the best performance, with the highest average accuracy (0.91) and AUC (0.90) among all models. The deep learning method using MRI radiomics has excellent diagnostic performance with a high accuracy in predicting MGMT promoter methylation in diffuse gliomas.
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http://dx.doi.org/10.3390/jcm11123445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224690PMC
June 2022

Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period.

Animals (Basel) 2022 Jun 10;12(12). Epub 2022 Jun 10.

Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.

is a neglected zoonotic roundworm distributed all over the world, causing toxocariasis in humans and animals. However, so far, the immune mechanism of infection in definitive hosts remains to be clarified. In this study, the transcriptional alterations of Beagle dogs' peripheral blood mononuclear cells (PBMCs) induced by infection during the lung infection period were analyzed using RNA-seq technology. A total of 2142 differentially expressed genes were identified, with 1066 upregulated genes and 1076 downregulated genes. Many differentially expressed genes participated in the biological process of intracellular signal transduction, as well as the immune- or inflammation-related KEGG signaling pathway, such as the Notch signaling pathway, Toll-like receptor signaling pathway, and NF-kappa B signaling pathway, through KEGG enrichment analysis. This study indicated that infection could suppress the biological function of Beagle dogs' PMBCs and provided basic data to further clarify the interaction mechanism between and host immune cells.
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http://dx.doi.org/10.3390/ani12121517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219457PMC
June 2022

Smart Antifreeze Hydrogels with Abundant Hydrogen Bonding for Conductive Flexible Sensors.

Gels 2022 Jun 13;8(6). Epub 2022 Jun 13.

National & Local Joint Engineering Research Center for Advanced Packaging Material and Technology, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.

Recently, flexible sensors based on conductive hydrogels have been widely used in human health monitoring, human movement detection and soft robotics due to their excellent flexibility, high water content, good biocompatibility. However, traditional conductive hydrogels tend to freeze and lose their flexibility at low temperature, which greatly limits their application in a low temperature environment. Herein, according to the mechanism that multi-hydrogen bonds can inhibit ice crystal formation by forming hydrogen bonds with water molecules, we used butanediol (BD) and N-hydroxyethyl acrylamide (HEAA) monomer with a multi-hydrogen bond structure to construct LiCl/p(HEAA--BD) conductive hydrogel with antifreeze property. The results indicated that the prepared LiCl/p(HEAA--BD) conductive hydrogel showed excellent antifreeze property with a low freeze point of -85.6 °C. Therefore, even at -40 °C, the hydrogel can still stretch up to 400% with a tensile stress of ~450 KPa. Moreover, the hydrogel exhibited repeatable adhesion property (~30 KPa), which was attributed to the existence of multiple hydrogen bonds. Furthermore, a simple flexible sensor was fabricated by using LiCl/p(HEAA--BD) conductive hydrogel to detect compression and stretching responses. The sensor had excellent sensitivity and could monitor human body movement.
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http://dx.doi.org/10.3390/gels8060374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223130PMC
June 2022

Identification and characterization of virus-encoded circular RNAs in host cells.

Microb Genom 2022 06;8(6)

School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China.

Emerging evidence has identified viral circular RNAs (circRNAs) in human cells infected by viruses, interfering with the immune system and inducing diseases including human cancer. However, the biogenesis and regulatory mechanisms of virus-encoded circRNAs in host cells remain unknown. In this study, we used the circRNA detection tool CIRI2 to systematically determine the virus-encoded circRNAs in virus-infected cancer cell lines and cancer patients, by analysing RNA-Seq datasets derived from RNase R-treated samples. Based on the thousands of viral circRNAs we identified, the biological characteristics and potential roles of viral circRNAs in regulating host cell function were determined. In addition, we developed a Viral-circRNA Database (http://www.hywanglab.cn/vcRNAdb/), which is open to all users to search, browse and download information on circRNAs encoded by viruses upon infection.
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http://dx.doi.org/10.1099/mgen.0.000848DOI Listing
June 2022

Demethoxycurcumin mitigates inflammatory responses in lumbar disc herniation via MAPK and NF-κB pathways in vivo and in vitro.

Int Immunopharmacol 2022 Jul 4;108:108914. Epub 2022 Jun 4.

School of Basic Medicine Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address:

The inflammatory radicular pain induced by lumbar disc herniation (LDH) is a serious problem worldwide. Demethoxycurcumin (DMC) is a yellow pigment derived from turmeric. Although it is considered a safe natural compound for managing inflammation-associated diseases, but the molecular mechanisms of LDH remain to be elucidated. In the current study, DMC reduced the production of IL-1β, IL-4, and IL-6 in nucleus pulposus (NP) cells subjected to TNF-α-induced inflammation. Moreover, the inhibitory mechanism was activated upon suppression of activation of MAPKs and NF-κB signalling in NP cells. Further experiments with LDH model rats supported the in vitro results. These studies expand our knowledge of the effect of DMC on LDH; DMC may be a viable alternative to the drugs used to treat LDH.
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http://dx.doi.org/10.1016/j.intimp.2022.108914DOI Listing
July 2022

Direct Crystallization Resolution of Racemates Enhanced by Chiral Nanorods: Experimental, Statistical, and Quantum Mechanics/Molecular Dynamics Simulation Studies.

ACS Omega 2022 Jun 3;7(23):19828-19841. Epub 2022 Jun 3.

Chemical Engineering Department, Frontier Medical Technologies Institute, Shanghai University of Engineering Science, Shanghai 201620, China.

Three chiral nanorods of C-l-Thea, C-l-Phe, and C-d-Phe were first synthesized and utilized as heterogeneous nucleants to enhance the resolution of racemic Asp via direct crystallization. Through the statistical analysis from 320 batches of nucleation experiments, we found that the apparent appearance diversity of two enantiomeric crystals of Asp existed in 80 homogeneous experiments without chiral nanorods. However, in 240 heterogeneous experiments with 4.0 wt % chiral nanorods of solute mass added, the appearance of those nuclei with the same chirality as the nanorods was apparently promoted, and that with the opposite chirality was totally inhibited. Under a supersaturation level of 1.08, the maximum ee of the initial nuclei was as high as 23.51%. When the cooling rate was 0.025 K/min, the ee of the product was up to 76.85% with a yield of 14.41%. Furthermore, the simulation results from quantum mechanics (QM) and molecular dynamics (MD) revealed that the higher chiral recognition ability of C-l-Thea compared to C-l-Phe that originated from the interaction difference between C-l-Thea and Asp enantiomers was larger than that between C-l-Phe and Asp enantiomers. Moreover, the constructed nanorods exhibited good stability and recyclability.
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http://dx.doi.org/10.1021/acsomega.2c01596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202296PMC
June 2022

Copper Doped Carbon Dots for Addressing Bacterial Biofilm Formation, Wound Infection, and Tooth Staining.

ACS Nano 2022 Jun 17. Epub 2022 Jun 17.

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, School of Materials Science and Engineering, School of Physics, South China University of Technology, Guangzhou 510640, China.

Oral infectious diseases and tooth staining, the main challenges of dental healthcare, are inextricably linked to microbial colonization and the formation of pathogenic biofilms. However, dentistry has so far still lacked simple, safe, and universal prophylactic options and therapy. Here, we report copper-doped carbon dots (Cu-CDs) that display enhanced catalytic (catalase-like, peroxidase-like) activity in the oral environment for inhibiting initial bacteria () adhesion and for subsequent biofilm eradication without impacting the surrounding oral tissues via oxygen (O) and reactive oxygen species (ROS) generation. Especially, Cu-CDs exhibit strong affinity for lipopolysaccharides (LPS) and peptidoglycans (PGN), thus conferring them with excellent antibacterial ability against Gram-positive bacteria () and Gram-negative bacteria (), such that they can prevent wound purulent infection and promoting rapid wound healing. Additionally, the Cu-CDs/HO system shows a better performance in tooth whitening, compared with results obtained with other alternatives, e.g., CDs and clinically used HO, particularly its negligible enamel and dentin destruction. It is anticipated that the biocompatible Cu-CDs presented in this work are a promising nano-mouthwash for eliminating oral pathogenic biofilms, prompting wound healing as well as tooth whitening, highlighting their significance in oral health management.
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http://dx.doi.org/10.1021/acsnano.2c02518DOI Listing
June 2022
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