Publications by authors named "Yue Wu"

1,238 Publications

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Quantitative Assessment of Age-dependent changes in Porphyrins from Fluorescence Images of Ultraviolet Photography by Image Processing.

Photodiagnosis Photodyn Ther 2021 Jun 12:102388. Epub 2021 Jun 12.

Graduate School of Science and Technology, Keio University, Yokohama, Japan. Electronic address:

Background: Skin is the first line of defense against harmful external environmental factors. Lots of skin flora living on the skin surface deal with skin health care and skin disease treatment and prevention, such as bacteria, which form the unique and complex skin micro-ecological system. Propionibacterium acnes (P. acnes) is a member of the anaerobic organisms, which is involved in the induction of skin acne and produce porphyrins that absorb ultraviolet light and emit red fluorescence in response. As a result, surveillance of the condition of the skin becomes more important in both the diagnosis of skin acne and the evaluation of therapeutic effects. A lot of measurement methods for single skin biophysical properties have been reported recently, due to the limitations such as complexity, expensiveness and time spent, objective and convenient method of monitoring the skin condition has received great interest. This study focused on the age-dependent changes in porphyrins for normal skin, and developed a novel algorithm to evaluate porphyrins using the fluorescence images by image processing quantitatively.

Materials And Methods: An extraction algorithm was proposed for the segmentation of porphyrin fluorescence images in OpenCV. The algorithm consisted primarily of preprocessing, conversion from RGB color space to HSV color space, and classification of fluorescence. There are 3595 healthy Japanese aged 16-85 years enrolled in the study and fluorescence images were acquired from their cheek sites under 375nm UV-LED excitation. Age-related fluorescence variation was conducted applying the algorithm implemented.

Results: A new extraction algorithm has been proposed with fluorescence image input and three indexes output, including the number of fluorescence, area of fluorescence, and mean intensity of fluorescence. Proposed algorithm was verified by three parameters, the accuracy, sensitivity, and precision, which refer to the ability of algorithm to detect the number of fluorescence correctly and repeatedly. The verification results were 71%, 72%, and 88% respectively, taking a validly fundamental step for skin health record and analysis. Furthermore, large-scale fluorescence image segmentation results revealed that similar trends were coming out for all three indexes in cheek as people get older. All the fluorescence number, area and mean intensity arrived at the highest at 30 years old and fell off since then.

Conclusion: The number, area, and fluorescence intensity of porphyrins can be extracted well from fluorescence images with the proposed algorithm in the study, which has the potential to support diagnose skin acne and predict skin conditions as an assisted tool. It is implicated that fluorescence status is influenced by age, which rises to the peak around 30 years old for normal cheek's skin.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102388DOI Listing
June 2021

Synthesis of SPIO Nanoparticles and the Subsequent Applications in Stem Cell Labeling for Parkinson's Disease.

Nanoscale Res Lett 2021 Jun 14;16(1):107. Epub 2021 Jun 14.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Road, Suzhou New District, Suzhou, 215163, China.

Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons in the midbrain, and the stem cell transplantation method provides a promising strategy for the treatment. In these studies, tracking the biological behaviors of the transplanted cells in vivo is essential for a basic understanding of stem cell function and evaluation of clinical effectiveness. In the present study, we developed a novel ultrasmall superparamagnetic iron oxide nanoparticles coating with the polyacrylic acid (PAA) and methoxypolyethylene glycol amine (PEG) by thermal decomposition method and a two-step modification. The USPIO-PAA/PEG NPs have a uniform diameter of 10.07 ± 0.55 nm and proper absorption peak of the corresponding ligands, as showed by TEM and FTIR. MTT showed that the survival of cells incubated with USPIO-PAA/PEG NPs remained above 96%. The synthesized USPIO-PAA/PEG had a good relaxation rate of 84.65 s Mm, indicating that they could be efficiently uptake and traced by MRI. Furthermore, the primary human adipose-derived stem cells (HADSCs) were characterized, labeled with USPIO-PAA/PEG and transplanted into the striatum of 6-hydroxydopamine (6-OHDA)-induced PD rat models. The labeled cells could be traced by MRI for up to 3 weeks after the transplantation surgery; moreover, transplantation with the labeled HADSCs significantly attenuated apomorphine-induced rotations in PD models and increased the number of the dopaminergic neurons in the substania nigra. Overall, the development of USPIO-PAA/PEG NPs provides a promising tool for in vivo tracing technique of cell therapy and identifies a novel strategy to track stem cells with therapeutic potential in PD.
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http://dx.doi.org/10.1186/s11671-021-03540-zDOI Listing
June 2021

Cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy vs. cytoreductive surgery alone for intrahepatic cholangiocarcinoma with peritoneal metastases: A retrospective cohort study.

Eur J Surg Oncol 2021 May 14. Epub 2021 May 14.

Department of Biliary Tract I, Eastern Hepatobiliary Surgery Hospital, No.225, Changhai Road, Yangpu District, Shanghai, 200433, PR China. Electronic address:

Background: Cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) has survival benefits in patients with intraperitoneal malignant lesions, but there is no study specific to intrahepatic cholangiocarcinoma (ICC).

Purpose: To compare the prognosis of patients with advanced ICC undergoing CRS + HIPEC compared with CRS alone.

Methods: This study was a retrospective cohort study of patients with advanced ICC treated at the Shanghai Eastern Hepatobiliary Surgery Hospital between 01/2014 and 12/2018. The patients were divided into either CRS + HIPEC or CRS group based on the treatment they received. Overall survival (OS), complications, hospital stay, biochemical indicators, tumor markers, and number of HIPEC were examined.

Results: There were 51 and 61 patients in the CRS + HIPEC and CRS groups, respectively. There were no differences between the groups regarding preoperative CA19-9 levels (421 ± 381 vs. 523 ± 543 U/mL, P = 0.208). The hospital stay was longer in the CRS + HIPEC group (22.2 ± 10.0 vs. 18.6 ± 7.6 days, P = 0.033). The occurrence of overall complications was similar in the two groups (37.2% vs. 34.4%, P = 0.756). The postoperative CA19-9 levels were lower in the CRS + HIPEC group compared with the CRS group (196 ± 320 vs. 337 ± 396 U/mL, P = 0.044). The median OS was longer in the CRS + HIPEC group than in the CRS group (25.53 vs. 11.17 months, P < 0.001). Compared with the CRS group, the CRS + HIPEC group showed a higher occurrence of leukopenia (7.8% vs. 0, P = 0.040) but a lower occurrence of total bilirubin elevation (15.7% vs. 37.7%, P = 0.032).

Conclusion: CRS + HIPEC could be a treatment option for patients with advanced ICC, with improved OS and similar complications and adverse events compared with CRS alone.
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http://dx.doi.org/10.1016/j.ejso.2021.05.014DOI Listing
May 2021

Objective and quantitative measurement of skin micro-relief by image analysis and application in age-dependent changes.

Skin Res Technol 2021 Jun 12. Epub 2021 Jun 12.

Faculty of Science and Technology, Keio University, Yokohama, Japan.

Background: Skin micro-relief has been researched by a variety of devices and methods, which usually are expensive or complicated. On the other hand, skin micro-relief relates to quite a few parameters, and it is hard to evaluate all of them at the same time. In the study, all parameters related to skin micro-relief are extracted and evaluated by image analysis.

Materials And Methods: Skin micro-relief evaluation was divided into four aspects: (a) Tamura features method was used to evaluate skin surface. (b) Morphological transform was applied to extract skin pores. (c) Watershed transform was applied to extract skin furrows. (d) labeling operation was used to evaluate the number, area and average area of skin closed polygons. Then, cheek images from 163 healthy Japanese females (0-70 years old) are analyzed to explore the age-dependent changes.

Results: Most parameters increased as age went on with significant differences, such as skin surface coarseness, contrast, skin pore number, area, average area, skin furrow width, skin closed polygon area and skin closed polygon average area. Skin coarseness has a strong correlation with pore area.

Conclusion: The method proposed in the study provided a comprehensive and effective assessment of skin micro-relief.
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http://dx.doi.org/10.1111/srt.13060DOI Listing
June 2021

Deep Neural Message Passing With Hierarchical Layer Aggregation and Neighbor Normalization.

IEEE Trans Neural Netw Learn Syst 2021 Jun 9;PP. Epub 2021 Jun 9.

As a unified framework for graph neural networks, message passing-based neural network (MPNN) has attracted a lot of research interest and has been shown successfully in a number of domains in recent years. However, because of over-smoothing and vanishing gradients, deep MPNNs are still difficult to train. To alleviate these issues, we first introduce a deep hierarchical layer aggregation (DHLA) strategy, which utilizes a block-based layer aggregation to aggregate representations from different layers and transfers the output of the previous block to the subsequent block, so that deeper MPNNs can be easily trained. Additionally, to stabilize the training process, we also develop a novel normalization strategy, neighbor normalization (NeighborNorm), which normalizes the neighbor of each node to further address the training issue in deep MPNNs. Our analysis reveals that NeighborNorm can smooth the gradient of the loss function, i.e., adding NeighborNorm makes the optimization landscape much easier to navigate. Experimental results on two typical graph pattern-recognition tasks, including node classification and graph classification, demonstrate the necessity and effectiveness of the proposed strategies for graph message-passing neural networks.
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http://dx.doi.org/10.1109/TNNLS.2021.3084319DOI Listing
June 2021

Solid-phase fluorescent BODIPY-peptide synthesis dipyrrin construction.

Chem Sci 2020 Sep 24;11(41):11266-11273. Epub 2020 Sep 24.

Department of Chemistry, Hong Kong Baptist University Kowloon Hong Kong SAR China

Traditional fluorescent peptide chemical syntheses hinge on the use of limited fluorescent/dye-taggable unnatural amino acids and entail multiple costly purifications. Here we describe a facile and efficient protocol for construction of dipyrrins on the N-terminus with 20 natural and five unnatural amino acids and the lysine's side chain of selected peptides/peptide drugs through Fmoc-based solid-phase peptide synthesis. The new strategy enables the direct formation of boron-dipyrromethene (BODIPY)-peptide conjugates from simple aldehyde and pyrrole derivatives without pre-functionalization, and only requires a single-time chromatographic purification at the final stage. As a model study, synthesized EBNA1-targeting demonstrates intact selectivity , responsive fluorescence enhancement, and higher light cytotoxicity due to the photo-generation of cytotoxic singlet oxygen. This work offers a novel practical synthetic platform for fluorescent peptides for multifaceted biomedical applications.
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http://dx.doi.org/10.1039/d0sc04849fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162834PMC
September 2020

Diverse energy metabolism patterns in females in Neodon fuscus, Lasiopodomys brandtii, and Mus musculus revealed by comparative transcriptomics under hypoxic conditions.

Sci Total Environ 2021 Aug 16;783:147130. Epub 2021 Apr 16.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China; School of Physical Education (Main campus), Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address:

The effects of global warming and anthropogenic disturbance force animals to migrate from lower to higher elevations to find suitable new habitats. As such migrations increase hypoxic stress on the animals, it is important to understand how plateau- and plain-dwelling animals respond to low-oxygen environments. We used comparative transcriptomics to explore the response of Neodon fuscus, Lasiopodomys brandtii, and Mus musculus skeletal muscle tissues to hypoxic conditions. Results indicate that these species have adopted different oxygen transport and energy metabolism strategies for dealing with a hypoxic environment. N. fuscus promotes oxygen transport by increasing hemoglobin synthesis and reduces the risk of thrombosis through cooperative regulation of genes, including Fga, Fgb, Alb, and Ttr; genes such as Acs16, Gpat4, and Ndufb7 are involved in regulating lipid synthesis, fatty acid β-oxidation, hemoglobin synthesis, and electron-linked transmission, thereby maintaining a normal energy supply in hypoxic conditions. In contrast, the oxygen-carrying capacity and angiogenesis of red blood cells in L. brandtii are promoted by genes in the CYP and COL families; this species maintains its bodily energy supply by enhancing the pentose phosphate pathway and mitochondrial fatty acid synthesis pathway. However, under hypoxia, M. musculus cannot effectively transport additional oxygen; thus, its cell cycle, proliferation, and migration are somewhat affected. Given its lack of hypoxic tolerance experience, M. musculus also shows significantly reduced oxidative phosphorylation levels under hypoxic conditions. Our results suggest that the glucose capacity of M. musculus skeletal muscle does not provide sufficient energy during hypoxia; thus, we hypothesize that it supplements its bodily energy by synthesizing ketone bodies. For the first time, we describe the energy metabolism pathways of N. fuscus and L. brandtii skeletal muscle tissues under hypoxic conditions. Our findings, therefore, improve our understanding of how vertebrates thrive in high altitude and plain habitats when faced with hypoxic conditions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147130DOI Listing
August 2021

Effect of -substitute group on the chirality of monocarboxylic acid stabilized CdSe nanocrystals.

Nanotechnology 2021 Jun 4. Epub 2021 Jun 4.

National Center for Nanoscience and Technology, Haidian District, 100190, CHINA.

The effect of -substitute groups at the asymmetric carbon of chiral monocarboxylic acid ligand, on the chirality of CdSe nanocrystals (NCs) was studied. When the substitution groups have strong electron-withdrawing capability, the CdSe NCs displayed an enhanced chirality where the g-factors were comparable to those with dicarboxylic chiral ligands. In addition, adding ethanol was demonstrated as an effective way to stabilize NCs, however, completely opposite g-factor evolution behaviour was found for NCs with different -substituted ligands. Specifically, the g-factor has increased/decreased with strong/weak electron-withdrawing -substitute groups probably due to the different intermolecular hydrogen bonding between carboxylic acids and ethanol.
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http://dx.doi.org/10.1088/1361-6528/ac084cDOI Listing
June 2021

The effect of high flow nasal oxygen therapy in intensive care units: A systematic review and meta-analysis.

Expert Rev Respir Med 2021 Jun 3. Epub 2021 Jun 3.

Zhujiang Hospital, Guangzhou, 510280 China.

Background: High flow nasal oxygen (HFNO) therapy has been widely used in intensive care units (ICU); however, its efficacy remains inconclusive. This systematic review and meta-analysis aimed to compare the efficacy of HFNO therapy with that of alternative noninvasive oxygen therapies such as conventional oxygen therapy (COT) and noninvasive ventilation (NIV) in ICU.

Methods: A Pubmed, Embase, Web of Science, Cochrane Library database search, which identified studies that assessed the outcomes of HFNO in adult ICU patients, was performed in March 2020. : The meta-analysis ultimately included 17 clinical studies, of which 11 were randomized control studies. Compared with the overall effect of COT and NIV, HFNO was associated with a low incidence of pneumonia (95% CI: 0.6-0.99, = 0.04) and improvement in lowest pulse oxygen saturation (SpO₂) during oxygenation (95% CI: 0.02-1.61; = 0.04). However, no differences were detected in the following outcomes: length of ICU stay, the rate of intubation or reintubation, mortality at day 28, hospital mortality, and SpO₂ at the end of oxygen therapy (all > 0.05).

Conclusions: In adult patients in ICU, HFNO may improve oxygenation and decrease pneumonia rate without affecting the length of ICU stay, intubation or reintubation rate, mortality, and SpO₂ at the end of oxygen therapy.
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http://dx.doi.org/10.1080/17476348.2021.1937131DOI Listing
June 2021

Crystallographic Characteristic Effect of Cu Substrate on Serrated Cathode Dissolution in Cu/Sn-3.0Ag-0.5Cu/Cu Solder Joints during Electromigration.

Materials (Basel) 2021 May 11;14(10). Epub 2021 May 11.

School of Materials Engineering, Lanzhou Institute of Technology, Lanzhou 730050, China.

The crystallographic characteristic effect of Cu substrate on cathode dissolution behavior in line-type Cu/Sn-3.0Ag-0.5Cu (SAC305)/Cu solder joints during electromigration (EM) was investigated by scanning electron microscope (SEM), electron backscatter diffraction (EBSD), and first-principles calculations. The SEM and EBSD results show that the crystallographic characteristic of Cu substrate is crucial to cathode dissolution behavior under a direct current of 1.5 × 10 A/cm at 125 °C ± 2 °C. When the (001) plane of copper grain adjacent to the CuSn/Cu interface is perpendicular or nearly perpendicular to the current direction, local cathode dissolution tips are easily formed, whereas the (111) plane remains mostly undissolved, which finally leads to the inhomogeneous cathode serrated dissolution in the substrate. The first-principles calculation results reveal that the different surface energies and energy barriers of the different crystallographic planes of Cu grains in the substrate are responsible for the local cathode dissolution tips. Adjusting the copper grain in a substrate to a crystal plane or direction that is difficult to dissolve during EM is a promising method for improving the reliability of solder joints in the future.
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http://dx.doi.org/10.3390/ma14102486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151550PMC
May 2021

HDL-C/apoA-I Ratio Is Associated with the Severity of Coronary Artery Stenosis in Diabetic Patients with Acute Coronary Syndrome.

Dis Markers 2021 18;2021:6689056. Epub 2021 May 18.

Department of Cardiovascular Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Background: Emerging evidence demonstrates that the lipid metabolism in acute coronary syndrome (ACS) patients with type 2 diabetes mellitus (T2DM) differs from nondiabetic patients. However, the distinct lipid profiles and their relationships with the severity of coronary artery stenosis and prognosis in patients with T2DM remain elusive.

Method And Result: This single-center, prospective cohort study enrolled 468 patients diagnosed with ACS undergoing coronary angiography, consisting of 314 non-DM and 154 DM patients. The HDL-C/apoA-I ratio was significantly higher in DM patients with a multivessel (≥3 affected vessels) lesion than a single-vessel (1-2 affected vessels) lesion. Regression analyses showed that the HDL-C/apoA-I ratio was positively correlated to the number of stenotic coronary arteries in DM patients but not non-DM patients. However, Kaplan-Meier survival analysis revealed no significant difference in the major adverse cardiovascular event rate regarding different HDL-C/apoA-I levels in DM or non-DM ACS patients at the end of the 2-year follow-up.

Conclusion: A higher HDL-C/apoA-I ratio is associated with increased severity of coronary artery stenosis in DM patients with ACS but not with the rate of major adverse cardiovascular events at the end of the 2-year follow-up.
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http://dx.doi.org/10.1155/2021/6689056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149224PMC
May 2021

A Novel Cross-Linked Hemoglobin-Based Oxygen Carrier, YQ23, Extended the Golden Hour for Uncontrolled Hemorrhagic Shock in Rats and Miniature Pigs.

Front Pharmacol 2021 12;12:652716. Epub 2021 May 12.

State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Daping Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Hypotensive resuscitation is widely applied for trauma and war injury to reduce bleeding during damage-control resuscitation, but the treatment time window is limited in order to avoid hypoxia-associated organ injury. Whether a novel hemoglobin-based oxygen carrier (HBOC), YQ23 in this study, could protect organ function, and extend the Golden Hour for treatment is unclear. Uncontrolled hemorrhagic shock rats and miniature pigs were infused with 0.5, 2, and 5% YQ23 before bleeding was controlled, while Lactate Ringer's solution (LR) and fresh whole blood plus LR (WB + LR) were set as controls. During hypotensive resuscitation the mean blood pressure was maintained at 50-60 mmHg for 60 min. Hemodynamics, oxygen delivery and utilization, blood loss, fluid demand, organ function, animal survival as well as side effects were observed. Besides, in order to observe whether YQ23 could extend the Golden Hour, the hypotensive resuscitation duration was extended to 180 min and animal survival was observed. Compared with LR, infusion of YQ23 in the 60 min pre-hospital hypotensive resuscitation significantly reduced blood loss and the fluid demand in both rats and pigs. Besides, YQ23 could effectively stabilize hemodynamics, and increase tissue oxygen consumption, increase the cardiac output, reduce liver and kidney injury, which helped to reduce the early death and improve animal survival. In addition, the hypotensive resuscitation duration could be extended to 180 min using YQ23. Side effects such as vasoconstriction and renal injury were not observed. The beneficial effects of 5% YQ23 are equivalent to similar volume of WB + LR. HBOC, such as YQ23, played vital roles in damage-control resuscitation for emergency care and benefited the uncontrolled hemorrhagic shock in the pre-hospital treatment by increasing oxygen delivery, reducing organ injury. Besides, HBOC could benefit the injured and trauma patients by extending the Golden Hour.
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http://dx.doi.org/10.3389/fphar.2021.652716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149754PMC
May 2021

Integrated analysis of microRNA and mRNA expression profiles in homozygous familial hypercholesterolemia patients and validation of atherosclerosis associated critical regulatory network.

Genomics 2021 May 28;113(4):2572-2582. Epub 2021 May 28.

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Homozygous familial hypercholesterolemia (HoFH) is a rare, life-threatening genetic disorder characterized by an extremely elevated serum level of low-density lipoprotein cholesterol (LDL-C) and accelerated premature atherosclerotic cardiovascular diseases (ASCVD). However, the detailed mechanism of how the pathogenic mutations of HoFH trigger the acceleration of ASCVD is not well understood. Therefore, we performed high-throughput RNA and small RNA sequencing on the peripheral blood RNA samples of six HoFH patients and three healthy controls. The gene and miRNA expression differences were analyzed, and seven miRNAs and six corresponding genes were screened out through regulatory network analysis. Validation through quantitative PCR of genes and miRNAs from 52 HoFH patients and 20 healthy controls revealed that the expression levels of hsa-miR-486-3p, hsa-miR-941, and BIRC5 were significantly upregulated in HoFH, while ID1, PLA2G4C, and CACNA2D2 were downregulated. Spearman correlation analysis found that the levels of ID1, hsa-miR-941, and hsa-miR-486-3p were significantly correlated with additional ASCVD risk factors in HoFH patients. This study represents the first integrated analysis of transcriptome and miRNA expression profiles in patients with HoFH, a rare disease, and as a result, six differentially expressed miRNAs/genes that may be related to atherosclerosis in HoFH are reported. The miRNA-mRNA regulatory network may be the critical regulation mechanism by which ASCVD is accelerated in HoFH.
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http://dx.doi.org/10.1016/j.ygeno.2021.05.036DOI Listing
May 2021

LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis.

Cancer Cell Int 2021 May 29;21(1):284. Epub 2021 May 29.

Department of Integrated Chinese and Western Medicine Oncology, The First Affiliated Hospital of Anhui Medical University, No. 218, Jixi Road, Hefei, 230022, Anhui, China.

Background: Ovarian cancer (OC) is a high-mortality gynecological cancer that is typically treated with cisplatin, although such treatment often results in chemoresistance. Ovarian cancer resistance is usually related to cell stemness. Herein, we explored the function of lncRNA WDFY3-AS2 in OC cell resistance to cisplatin (DDP).

Methods: Cisplatin resistant OC A2780 cell lines (A2780-DDP) were established by long-term exposure to cisplatin. CCK-8 assay were performed to evaluate the viability of A2780, and A2780-DDP cells. Quantitative RT-PCR was used to examine the expression of lncRNA WDFY3-AS2, miR-139-5p, and SDC4 in A2780-DDP cell lines. After treatment with cisplatin, cell apoptosis and CD44CD166-positive cells were measured by flow cytometry. The transwell assays were employed to measure the effect of WDFY3-AS2 on cell migration, and invasion. In addition, tumorsphere formation assay was used to enrich OC cancer stem cells (CSCs) from A2780-DDP cells. The expression of CSC markers (SOX2, OCT4, and Nanog) was detected by western blotting. The regulatory mechanism was confirmed by RNA pull down, and luciferase reporter assays. Furthermore, xenograft tumor in nude mice was used to assess the impact of WDFY3-AS2 on cisplatin resistance in OC in vivo.

Results: WDFY3-AS2 was highly expressed in OC A2780-DDP cells, and silencing WDFY3-AS2 significantly inhibited proliferation, migration and invasion but increased apoptosis in OC A2780-DDP cells. Additionally, WDFY3-AS2 significantly promoted the A2780-DDP cells tumorspheres. WDFY3-AS2 was predicted to impact OC by sponging miR-139-5p and regulating SDC4. The xenografts inoculated with A2780-DDP cells additionally confirmed that tumor growth in vivo was reduced by si-WDFY3-AS2 transfection. MiR-139-5p inhibitor or SDC4 overexpression could restore the suppressive influence of silenced WDFY3-AS2 on tumor growth.

Conclusions: Together, WDFY3-AS2 may lead to change of cisplatin resistance by the expression of miR-139-5p/SDC4 in the OC A2870-DDP cells both in vitro and in vivo. Our finding may provide a drug target for the drug resistance of OC.
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http://dx.doi.org/10.1186/s12935-021-01993-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164817PMC
May 2021

The role of AQP3 in the vascular leakage of sepsis and the protective effect of Ss-31.

J Cardiovasc Pharmacol 2021 May 27. Epub 2021 May 27.

State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, People's Republic of China.

Abstract: Aquaporins(AQPs) are a group of membrane proteins related to water permeability. Studies have shown AQPs play a vital role in various diseases. Whether AQPs participate in regulating vascular permeability after sepsis and the subtype of AQPs is related are unknown. Ss-31, as a new antioxidant, had protective effects on a variety of diseases. However, whether Ss-31 has a protective effect on pulmonary vascular permeability in sepsis and whether its effect is related to AQPs is unclear. Using the cecum ligation puncture (CLP) induced septic rat and LPS treated pulmonary vein endothelial cells(PVECs), the role of AQPs in the regulation of the permeability of pulmonary vascular, and its relationship to Ss-31 were studied. The results showed that the pulmonary vascular permeability significantly increased after sepsis, meanwhile the expressions of AQP3, 4, and 12 increased. Among those, the AQP3 was closely correlated with pulmonary vascular permeability. Inhibition of AQP3 antagonized the increase of the permeability of monolayer PVECs. Further study showed that the expression of Caveolin-1(Cav-1) increased and Occludin decreased after sepsis. Inhibition of AQP3 antagonized the decrease of Cav-1 and the increase of Occludin in sepsis. Antioxidant Ss-31 decreased the expression of AQP3 and ROS levels. At the same time, Ss-31 improved pulmonary vascular permeability and prolonged survival of sepsis rats. In conclusion, AQP3 participates in the regulation of pulmonary vascular permeability after sepsis, and the antioxidant Ss-31 has a protective effect on pulmonary vascular permeability by down-regulating the expression of AQP3 and inhibiting ROS production.
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http://dx.doi.org/10.1097/FJC.0000000000001050DOI Listing
May 2021

Mesenchymal stem cell-derived microvesicles improve intestinal barrier function by restoring mitochondrial dynamic balance in sepsis rats.

Stem Cell Res Ther 2021 May 26;12(1):299. Epub 2021 May 26.

State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department, Research Institute of Surgery, Daping Hospital, Army Medical University, Daping, Chongqing, 400042, People's Republic of China.

Background: Sepsis is a major cause of death in ICU, and intestinal barrier dysfunction is its important complication, while the treatment is limited. Recently, mesenchymal stem cell-derived microvesicles (MMVs) attract much attention as a strategy of cell-free treatment; whether MMVs are therapeutic in sepsis induced-intestinal barrier dysfunction is obscure.

Methods: In this study, cecal ligation and puncture-induced sepsis rats and lipopolysaccharide-stimulated intestinal epithelial cells to investigate the effect of MMVs on intestinal barrier dysfunction. MMVs were harvested from mesenchymal stem cells and were injected into sepsis rats, and the intestinal barrier function was measured. Afterward, MMVs were incubated with intestinal epithelial cells, and the effect of MMVs on mitochondrial dynamic balance was measured. Then the expression of mfn1, mfn2, OPA1, and PGC-1α in MMVs were measured by western blot. By upregulation and downregulation of mfn2 and PGC-1α, the role of MMVs in mitochondrial dynamic balance was investigated. Finally, the role of MMV-carried mitochondria in mitochondrial dynamic balance was investigated.

Results: MMVs restored the intestinal barrier function by improving mitochondrial dynamic balance and metabolism of mitochondria. Further study revealed that MMVs delivered mfn2 and PGC-1α to intestinal epithelial cells, and promoted mitochondrial fusion and biogenesis, thereby improving mitochondrial dynamic balance. Furthermore, MMVs delivered functional mitochondria to intestinal epithelial cells and enhanced energy metabolism directly.

Conclusion: MMVs can deliver mfn2, PGC-1α, and functional mitochondria to intestinal epithelial cells, synergistically improve mitochondrial dynamic balance of target cells after sepsis, and restore the mitochondrial function and intestinal barrier function. The study illustrated that MMVs might be a promising strategy for the treatment of sepsis.
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http://dx.doi.org/10.1186/s13287-021-02363-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152336PMC
May 2021

Locally restricted glucose availability in the embryonic hypocotyl determines seed germination under ABA treatment.

New Phytol 2021 May 25. Epub 2021 May 25.

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Abiotic stresses affect plant growth and development by causing cellular damage and/or restricting resources. Plants respond to stresses often through abscisic acid (ABA) signaling for survival. Thus, ABA application largely mimics stress responses, which can be overridden by glucose (Glc) addition during seed germination. It remains unclear whether ABA-mediated germination inhibition is due to regional or global suppression of Glc availability in the germinating Arabidopsis seeds. We used a genetically engineered FRET sensor to evaluate if ABA affects the spatiotemporal distribution of Glc, C-Glc uptake assays to track potential effects of ABA on sugar import, and transcriptome and mutant analyses to identify genes associated with Glc availability involved in ABA-inhibited seed germination. ABA limits Glc in the hypocotyl largely due to suppressing sugar allocation along with altering sugar metabolism. Loss-of-function mutants of ABA-inducible sucrose-phosphate synthase (SPS) genes accumulated more Glc, leading to germination insensitive to ABA. We reveal that Glc antagonizes ABA by globally counteracting the ABA influence at the transcript level, including expansin (EXP) family genes suppressed by ABA. This study presents a new perspective on how ABA affects Glc distribution, which likely reflects what occurs when seeds are subject to abiotic stresses, such as drought and salt stress.
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http://dx.doi.org/10.1111/nph.17513DOI Listing
May 2021

Mechanism of low-frequency and high-frequency ultrasound-induced inactivation of soy trypsin inhibitors.

Food Chem 2021 Oct 11;360:130057. Epub 2021 May 11.

Algal Processing Group, Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address:

In this study, the effect of ultrasonic frequency and power on the inactivation of soy trypsin inhibitors (TIs) was investigated to explore the ultrasound-induced inactivation mechanism. It was observed that 20 kHz and 355 kHz ultrasound have better inactivation efficiency than 1056 kHz. First-order rate constants for the inactivation process were obtained, which increased with increasing ultrasonic power at both 20 kHz and 355 kHz. For 20 kHz ultrasound, the formation of TI aggregates resulting from the physical effects of acoustic cavitation decreased the interactions between the active sites of TIs and trypsin, thus reducing the TI activity. For 355 kHz ultrasound, most of the methionine in the TIs was oxidised within 5 mins, resulting in a faster reduction of TI activity. Subsequent aggregation of TIs resulted in further TI inactivation. SDS-PAGE showed that neither disulphide bonds nor CC coupling were involved in the formation of aggregates.
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http://dx.doi.org/10.1016/j.foodchem.2021.130057DOI Listing
October 2021

Inhibition of the Oxygen-Sensing Asparaginyl Hydroxylase Factor Inhibiting Hypoxia-Inducible Factor: A Potential Hypoxia Response Modulating Strategy.

J Med Chem 2021 06 24;64(11):7189-7209. Epub 2021 May 24.

Jiangsu Key Laboratory of Drug Design and Optimization, and Department of Chemistry, China Pharmaceutical University, Nanjing 211198, China.

Factor inhibiting hypoxia-inducible factor (FIH) is a JmjC domain 2-oxogluarate and Fe(II)-dependent oxygenase that catalyzes hydroxylation of specific asparagines in the C-terminal transcriptional activation domain of hypoxia-inducible factor alpha (HIF-α) isoforms. This modification suppresses the transcriptional activity of HIF by reducing its interaction with the transcriptional coactivators p300/CBP. By contrast with inhibition of the HIF prolyl hydroxylases (PHDs), inhibitors of FIH, which accepts multiple non-HIF substrates, are less studied; they are of interest due to their potential ability to alter metabolism (either in a HIF-dependent and/or -independent manner) and, provided HIF is upregulated, to modulate the course of the HIF-mediated hypoxic response. Here we review studies on the mechanism and inhibition of FIH. We discuss proposed biological roles of FIH including its regulation of HIF activity and potential roles of FIH-catalyzed oxidation of non-HIF substrates. We highlight potential therapeutic applications of FIH inhibitors.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00415DOI Listing
June 2021

The therapeutic value of arthroscopic microfracture technique in combination with platelet-rich plasma injection for knee cartilage injury.

Am J Transl Res 2021 15;13(4):2694-2701. Epub 2021 Apr 15.

Trauma Department of Orthopedics, The First Affiliated Hospital of University of South China Hengyang City, Hunan Province, China.

Objective: The purpose of this study was to analyze the efficacy of platelet-rich plasma (PRP) injection combined with arthroscopic microfracture technique for knee cartilage injury.

Methods: Seventy-nine patients with knee cartilage injury were randomly divided into a control group (CG, n=39) and an observation group (OBG, n=40). Both of the groups were treated with the arthroscopic microfracture technique, and the OBG was additionally treated with PRP injection.

Results: The VAS scores for pain in the affected area of the OBG were lower than those of the CG at 1, 3, 5, and 7 days after surgery ( < 0.05). Knee flexion, hyperextension, and rotation angles in the OBG were greater than those in the CG at 1 month after surgery ( < 0.05). IKDC scores in the OBG were lower than those in the CG at 1, 2, and 3 weeks after surgery ( < 0.05). The Tegner and Lysholm scores in the OBG were higher than those in the CG at 1, 2, and 3 months after surgery ( < 0.05). The complication rate in the OBG was 10.00%, which was lower than that of 28.21% in the CG ( < 0.05).

Conclusion: The efficacy of microfracture technique combined with PRP injection in the treatment of knee joint cartilage injury is significantly improved compared with that of microfracture technique alone, which can reduce postoperative complications and improve the range of motion and function of the knee joint.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129346PMC
April 2021

Effects of metoprolol on serum inflammatory factors and myocardial ischemia in rats modeled with coronary heart disease.

Am J Transl Res 2021 15;13(4):2518-2527. Epub 2021 Apr 15.

Department of Cardiology, Xingtai People's Hospital Xingtai 054001, Hebei Province, China.

Objective: This study was designed to observe the effects of metoprolol on serum inflammatory factors, cardiac function and oxidative stress response in rats modeled with coronary heart disease (CHD).

Methods: Thirty clean SD rats aged 6-8 weeks were randomized into a control group (CG), treatment group (TG) and model group (MG), with 10 in each group. Rats in the CG were fed regular chow, while those in the MG and TG were fed a high-fat diet. After successful CHD modeling, those in the TG were given metoprolol every day, 10 mg/kg once a day. The effects of cardiac function indexes, myocardial injury indexes, blood lipids, inflammatory factors and oxidative stress indexes, myocardial apoptosis-related factors and apoptosis rate were observed and recorded before and after treatment.

Results: Compared with the CG, the cardiac function indexes of the MG decreased significantly, while the myocardial injury indexes increased markedly. After metoprolol treatment, the cardiac function and myocardial injury of the TG were significantly improved. Also, the expression of serum lipid indexes in the MG increased obviously, and the hyperlipidemia in the TG was improved after metoprolol treatment. Besides, the expression of inflammatory factors in serum of the MG increased remarkably, and metoprolol could reduce the inflammatory state in rats. Furthermore, MDA in serum of the MG increased, SOD, CAT, GSH-Px decreased; revealing that metoprolol can improve oxidative stress in rats. Finally, the apoptosis rate of cardiomyocytes in the MG increased dramatically. Metoprolol treatment can reduce the apoptosis rate and improve the expression of apoptosis related proteins.

Conclusion: Metoprolol reduces the degree of myocardial injury, inhibits inflammatory reaction and oxidative stress , reduces myocardial apoptosis and improves myocardial ischemia in CHD modeled rats.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129274PMC
April 2021

The genomic distribution map of human papillomavirus in Western China.

Epidemiol Infect 2021 May 18;149:e135. Epub 2021 May 18.

Clinical Molecular Medicine Testing Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing400016, China.

Human papillomavirus (HPV) has been confirmed as the causative agent for cervical cancer. In this study, a total of 301 880 women were recruited from four different regions of Western China, with 301 880 exfoliated cervical cell samples collected from women for DNA isolation and purification. The HPV genotype was tested by polymerase chain reaction. The overall HPV prevalence rate, high-risk (HR) HPV infection rate, low-risk (LR) HPV infection rate and mixed HPV infection rate was 18.24%, 79.14%, 12.56% and 8.30%, respectively. The four most common HR HPV subtypes were HPV-52, 16, 58 and 53, which accounted for 20.49%, 19.93%, 14.54% and 10.01%, respectively. In LR HPV genotype, HPV-6 ranked the highest (28.17%), followed by HPV-81 (9.09%) and HPV-11 (3.78%). HPV genotype subgroup analysis also showed that single-type infection was the most common (77.26%) among HPV-positive individuals. Among multi-infection genotypes, double infection was the most common with frequencies of 76.04%. The overall prevalence of HPV is high in Western China, whose distribution demonstrates different patterns across different ages and regions. Viral genotypes HPV 53, 6 were frequently detected in this population, which is worth of significant clinical attention.
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http://dx.doi.org/10.1017/S0950268821001175DOI Listing
May 2021

A Comprehensive Evaluation of Township Hospitals in the Severely Cold Areas of China.

HERD 2021 May 18:19375867211010268. Epub 2021 May 18.

Key Laboratory of Cold Region Urban and Rural Human Settlement Environment Science and Technology, School of Architecture, 47822Harbin Institute of Technology, China.

Objective: The aims of this study are to establish an evaluation system and find the existing problems in the architectural design of township hospitals in the severely cold regions of China.

Background: Due to the geographical location and economic factors, development still has some key problems, such as inadequate construction, old facilities, and backward technology, which are more prominent in the severely cold regions.

Methods: First, evaluation factors have been selected and determined by literature review and on-site investigation. Evaluation rules have been determined using fuzzy membership function. Then, the analytic hierarchy process is used to determine the weights of the evaluation factors at all levels. Finally, take a township hospital as an example to calculate the comprehensive evaluation results.

Results: A comprehensive evaluation index system with 28 elements and 76 factors for the township hospitals in severely cold regions including the basic health service capacity, the emergency capacity, and the climate fitness.

Conclusions: The establishment of the comprehensive evaluation system of township hospitals in severely cold areas in this study aims to find out the problems through the evaluation of the existing township hospitals in the severely cold areas and provide guidance for the transformation of existing township hospitals in the severely cold areas. By comparing the scheme evaluation for building hospitals, we shall look for the optimal solution to provide reference for future development in the construction of township hospitals.
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http://dx.doi.org/10.1177/19375867211010268DOI Listing
May 2021

Changes of coagulation function and risk of stroke in patients with COVID-19.

Brain Behav 2021 May 16:e02185. Epub 2021 May 16.

Department of Respiratory Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu Province, China.

Background And Purpose: COVID-19 is spreading throughout the whole world as a public health issue. There is a link between the new coronavirus and changes in biochemical indicators, such as coagulation functions. Hypercoagulable state of blood caused by infections may lead to cerebrovascular diseases. More attention should be paid to patients with COVID-19, especially critically ill individuals with history of cerebrovascular disease who may have high risk of stroke.

Methods: 193 patients with COVID-19 were enrolled in the study. These patients were categorized into nonsevere (143 patients) and severe (50 patients) groups. This study evaluated laboratory tests, including routine blood tests, C-reactive protein, erythrocyte sedimentation rate, electrolytes, and coagulation functions. Furthermore, neurological function and stroke risks were evaluated in this study.

Results: Compared to the nonsevere group, there were increases in white blood cells, neutrophil count, interleukin-6, erythrocyte sedimentation rate, and C-reactive protein in the severe group (p < .05). For coagulation functions, parameters like prothrombin time, international normalized ratio, activated partial thromboplastin time, thrombin time, D-dimer, and fibrin degradation products were increased significantly in the severe group (p < .01). Severe patients also demonstrated higher scores on the Framingham stroke risk profile and lower Glasgow scores (p < .05). Furthermore, significant associations were noticed between stroke risk and age, blood cell count, neutrophil count, D-dimmer, and fibrin degradation productions (p < .05).

Conclusions: Data suggested that coagulation functions were affected in patients with COVID-19. Hypercoagulable state in patients may lead to potential high risk of stroke.
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http://dx.doi.org/10.1002/brb3.2185DOI Listing
May 2021

Integrated Analysis to Identify a Redox-Related Prognostic Signature for Clear Cell Renal Cell Carcinoma.

Oxid Med Cell Longev 2021 21;2021:6648093. Epub 2021 Apr 21.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030 Hubei, China.

The imbalance of the redox system has been shown to be closely related to the occurrence and progression of many cancers. However, the biological function and clinical significance of redox-related genes (RRGs) in clear cell renal cell carcinoma (ccRCC) are unclear. In our current study, we downloaded transcriptome data from The Cancer Genome Atlas (TCGA) database of ccRCC patients and identified the differential expression of RRGs in tumor and normal kidney tissues. Then, we identified a total of 344 differentially expressed RRGs, including 234 upregulated and 110 downregulated RRGs. Fourteen prognosis-related RRGs (, , , , , , , , , , , , , and ) were selected out, and a prognosis-related signature was constructed based on these RRGs. Survival analysis showed that overall survival was lower in the high-risk group than in the low-risk group. The area under the receiver operating characteristic curve of the risk score signature was 0.728 at three years and 0.759 at five years in the TCGA cohort and 0.804 at three years and 0.829 at five years in the E-MTAB-1980 cohort, showing good predictive performance. In addition, we explored the regulatory relationships of these RRGs with upstream miRNA, their biological functions and molecular mechanisms, and their relationship with immune cell infiltration. We also established a nomogram based on these prognostic RRGs and performed internal and external validation in the TCGA and E-MTAB-1980 cohorts, respectively, showing an accurate prediction of ccRCC prognosis. Moreover, a stratified analysis showed a significant correlation between the prognostic signature and ccRCC progression.
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http://dx.doi.org/10.1155/2021/6648093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084660PMC
May 2021

CBP-JMF: An Improved Joint Matrix Tri-Factorization Method for Characterizing Complex Biological Processes of Diseases.

Front Genet 2021 23;12:665416. Epub 2021 Apr 23.

School of Computer Science and Technology, Xidian University, Xi'an, China.

Multi-omics molecules regulate complex biological processes (CBPs), which reflect the activities of various molecules in living organisms. Meanwhile, the applications to represent disease subtypes and cell types have created an urgent need for sample grouping and associated CBP-inferring tools. In this paper, we present CBP-JMF, a practical tool primarily for discovering CBPs, which underlie sample groups as disease subtypes in applications. Differently from existing methods, CBP-JMF is based on a joint non-negative matrix tri-factorization framework and is implemented in Python. As a pragmatic application, we apply CBP-JMF to identify CBPs for four subtypes of breast cancer. The result shows significant overlapping between genes extracted from CBPs and known subtype pathways. We verify the effectiveness of our tool in detecting CBPs that interpret subtypes of disease.
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http://dx.doi.org/10.3389/fgene.2021.665416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103031PMC
April 2021

Targeting PAR2 Overcomes Gefitinib Resistance in Non-Small-Cell Lung Cancer Cells Through Inhibition of EGFR Transactivation.

Front Pharmacol 2021 22;12:625289. Epub 2021 Apr 22.

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China.

Drug resistance can notably restrict clinical applications of gefitinib that is a commonly used EGFR-tyrosine kinase inhibitors (EGFR-TKIs) for non-small cell lung cancer (NSCLC). The attempts in exploring novel drug targets and reversal strategies are still needed, since gefitinib resistance has not been fully addressed. Protease-activated receptor 2 (PAR2), a G protein-coupled receptor, possesses a transactivation with EGFR to initiate a variety of intracellular signal transductions, but there is a lack of investigations on the role of PAR2 in gefitinib resistance. This study established that protease-activated receptor 2 (PAR2), actively participated in NSCLC resistant to gefitinib. PAR2 expression was significantly up-regulated when NSCLC cells or tumor tissues became gefitinib resistance. PAR2 inhibition notably enhanced gefitinib to modulate EGFR transactivation, cell viability, migration and apoptosis in gefitinib-sensitive and-resistant NSCLC cells, suggesting its reversal effects in gefitinib resistance. Meanwhile, the combination of a PAR2 inhibitor (P2pal-18S) and gefitinib largely blocked ERK phosphorylation and epithelial-mesenchymal transition (EMT) compared to gefitinib alone. Importantly, we probed its underlying mechanism and uncovered that PAR2 blockade sensitized gefitinib and reversed its resistance mainly via β-arrestin-EGFR-ERK signaling axis. These effects of PAR2 inhibition were further confirmed by the study which showed that P2pal-18S reactivated gefitinib to inhibit tumor growth via restricting ERK activation. Taken together, this study could not only reveal a new mechanism of receptor-mediated transactivation to modulate drug resistance, but also provide a novel drug target and direction for overcoming gefitinib resistance in NSCLC.
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http://dx.doi.org/10.3389/fphar.2021.625289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100583PMC
April 2021

Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection.

Immunity 2021 06 4;54(6):1200-1218.e9. Epub 2021 May 4.

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA; Department of Immunology, Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA. Electronic address:

Tissue macrophages self-renew during homeostasis and produce inflammatory mediators upon microbial infection. We examined the relationship between proliferative and inflammatory properties of tissue macrophages by defining the impact of the Wnt/β-catenin pathway, a central regulator of self-renewal, in alveolar macrophages (AMs). Activation of β-catenin by Wnt ligand inhibited AM proliferation and stemness, but promoted inflammatory activity. In a murine influenza viral pneumonia model, β-catenin-mediated AM inflammatory activity promoted acute host morbidity; in contrast, AM proliferation enabled repopulation of reparative AMs and tissue recovery following viral clearance. Mechanistically, Wnt treatment promoted β-catenin-HIF-1α interaction and glycolysis-dependent inflammation while suppressing mitochondrial metabolism and thereby, AM proliferation. Differential HIF-1α activities distinguished proliferative and inflammatory AMs in vivo. This β-catenin-HIF-1α axis was conserved in human AMs and enhanced HIF-1α expression associated with macrophage inflammation in COVID-19 patients. Thus, inflammatory and reparative activities of lung macrophages are regulated by β-catenin-HIF-1α signaling, with implications for the treatment of severe respiratory diseases.
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http://dx.doi.org/10.1016/j.immuni.2021.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192557PMC
June 2021

Association of the rs17250932, rs4794067, and rs2240017 polymorphism in the TBX21 gene with autoimmune diseases: A meta-analysis.

Allergol Immunopathol (Madr) 2021 1;49(3):83-90. Epub 2021 May 1.

Department of Epidemiology and Biostatistics, School of Public Health and Anhui Province Laboratory of Inflammation and Immune Mediated Diseases, Anhui Medical University, Hefei, Anhui, P.R. China;

Objective: To evaluate systematically the association between gene polymorphisms (rs17250932, rs2240017, and rs4794067) and the risk of autoimmune diseases in Asian populations.

Methods: The Medline, Web of Science, and Chinese Biomedical Literature Database were used to retrieve eligible studies that were published before July 2020. Pooled odds ratios (OR) and 95% confidence intervals (95% CI) were calculated by using the dominant model, heterozygote contrast model, and allelic contrast model. Publication bias was evaluated using contour-enhanced funnel plots and Egger's regression test. Sensitivity analysis was conducted to assess the robustness of this meta-analysis.

Results: A total of 12 eligible studies, including 3834 patients and 4824 healthy controls, were recruited in this meta-analysis. The pooled data demonstrated that rs2240017 and rs4794067 polymorphisms were significantly associated with the risk of autoimmune diseases in Asian populations in allelic contrast model (OR: 1.456, 95% CI: 1.131-1.875, P = 0.004; OR: 0.766, 95% CI: 0.615-0.954, P = 0.017), heterozygote comparison model (OR: 1.647, 95% CI: 1.239-2.189, P = 0.001; OR: 0.796, 95% CI: 0.634-0.999, P = 0.049), and dominant model (OR: 1.572, 95% CI: 1.194-2.071, P = 0.004; OR: 0.767, 95% CI: 0.607-0.970, P = 0.027). The G allele of rs2240017 may be a risk factor for autoimmune diseases, and the T allele of rs4794067 may increase the risk of autoimmune diseases. However, we failed to find evidence of the association between rs17250932 polymorphism and susceptibility to autoimmune diseases. No publication bias was established in this meta-analysis.

Conclusion: This meta-analysis indicated that rs2240017 and rs4794067 polymorphism confer susceptibility to autoimmune diseases, but not rs17250932.
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http://dx.doi.org/10.15586/aei.v49i3.80DOI Listing
May 2021

Prediction of the Growth Rate of Early-Stage Lung Adenocarcinoma by Radiomics.

Front Oncol 2021 15;11:658138. Epub 2021 Apr 15.

Department of Radiology, Huadong Hospital Affiliated With Fudan University, Shanghai, China.

Objectives: To investigate the value of imaging in predicting the growth rate of early lung adenocarcinoma.

Methods: From January 2012 to June 2018, 402 patients with pathology-confirmed lung adenocarcinoma who had two or more thin-layer CT follow-up images were retrospectively analyzed, involving 407 nodules. Two complete preoperative CT images and complete clinical data were evaluated. Training and validation sets were randomly assigned according to an 8:2 ratio. All cases were divided into fast-growing and slow-growing groups. Researchers extracted 1218 radiomics features from each volumetric region of interest (VOI). Then, radiomics features were selected by repeatability analysis and Analysis of Variance (ANOVA); Based on the Univariate and multivariate analyses, the significant radiographic features is selected in training set. A decision tree algorithm was conducted to establish the radiographic model, radiomics model and the combined radiographic-radiomics model. Model performance was assessed by the area under the curve (AUC) obtained by receiver operating characteristic (ROC) analysis.

Results: Sixty-two radiomics features and one radiographic features were selected for predicting the growth rate of pulmonary nodules. The combined radiographic-radiomics model (AUC 0.78) performed better than the radiographic model (0.727) and the radiomics model (0.710) in the validation set.

Conclusions: The model has good clinical application value and development prospects to predict the growth rate of early lung adenocarcinoma through the combined radiographic-radiomics model.
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http://dx.doi.org/10.3389/fonc.2021.658138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082461PMC
April 2021