Publications by authors named "Yue Shi"

389 Publications

Quality of life in ambulatory pulmonary arterial hypertension in connective tissue diseases and its relationship with risk stratification.

Pulm Circ 2021 Jul-Sep;11(3):20458940211029899. Epub 2021 Jul 11.

Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, National Clinical Research Center for Dermatologic and Immunologic Diseases, Ministry of Science & Technology, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

The Pulmonary Arterial Hypertension Symptoms and Impact Questionnaire (PAH-SYMPACT) is a PAH-specific patient-reported outcome scale assessing patients' quality of life from four aspects: cardiopulmonary symptoms, cardiovascular symptoms, physical impacts and cognitive/emotional impacts. This study aimed to validate the Chinese version of PAH-SYMPACT and explore its relationship with risk stratification in patients with connective tissue disease-associated pulmonary arterial hypertension (CTD-PAH). In addition, 75 patients with CTD-PAH confirmed by right heart catheterization were invited to complete questionnaires including PAH-SYMPACT, the 36-item Medical Outcomes Study Short Form Survey (SF-36) and EuroQol five dimensions questionnaire (EQ-5D). The demographic, clinical, laboratory and treatment data were collected. The endpoint was treatment goal achievement status in 6-12 months after completing the questionnaires, defined as an integrated outcome. Participants' mean age was 36.4 ± 11.9 years and the mean pulmonary arterial pressure was 38.9 ± 13.67 mmHg. The reliability of the PAH-SYMPACT domains ranged from 0.83 to 0.88. Results of factor analysis basically conformed the original PAH-SYMPACT. The treatment goal achievement (TGA) status in 6-12 months was significantly associated with physical impacts scores (odds ratio: 0.180, 95% confidence interval: 0.036-0.908, P=0.038). The Chinese version of PAH-SYMPACT is a reliable measurement to evaluate quality of life in CTD-PAH patients and is also a potential predictor of patient's condition change in routine clinical practice.
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http://dx.doi.org/10.1177/20458940211029899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278470PMC
July 2021

Adaptively controlled fast production of defect-free beryllium ion crystals using pulsed laser ablation.

Rev Sci Instrum 2021 Jun;92(6):063201

Department of Physics, New York University, New York, New York 10003, USA.

Trapped atomic ions find wide applications ranging from precision measurement to quantum information science and quantum computing. Beryllium ions are widely used due to the light mass and convenient atomic structure of beryllium; however, conventional ion loading from thermal ovens exerts undesirable gas loads for a prolonged duration. Here, we demonstrate a method to rapidly produce pure linear chains of beryllium ions with pulsed laser ablation, serving as a starting point for large-scale quantum information processing. Our method is fast compared to thermal ovens, reduces the gas load to only 10 Torr (10 Pa) level, yields a short recovery time of a few seconds, and also eliminates the need for a deep ultraviolet laser for photoionization. We also study the loading dynamics, which show non-Poissonian statistics in the presence of sympathetic cooling. In addition, we apply feedback control to obtain defect-free ion chains with desirable lengths.
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http://dx.doi.org/10.1063/5.0044372DOI Listing
June 2021

Competition between spin density wave and charge density wave driven by interactions of spinful Haldane model on honeycomb lattices.

J Phys Condens Matter 2021 Jul 26;33(39). Epub 2021 Jul 26.

School of Sciences, Xi'an Technological University, Xi'an 710032, People's Republic of China.

We investigate the interacting spinful Haldane model with on-site and nearest-neighbor repulsions on honeycomb lattices. Interactions drive spin-density and charge-density waves competing with each other. This competition enriches the phase diagram of the system greatly, where both topological and non-topological coexistence phases emerge with spin-density and charge-density wave orders. Moreover, the topological phase transition accompanied by the energy gap closure in coexistence phases is of third order. Finally, we study the topological properties consisting ofnumbers and edge states for different phases.
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http://dx.doi.org/10.1088/1361-648X/ac1300DOI Listing
July 2021

Pharmacokinetic and tissue distribution study of ZCY-15, a novel compound against Alzheimer's disease, in rats by liquid chromatography-tandem mass spectrometry.

Eur J Pharm Sci 2021 Sep 24;164:105917. Epub 2021 Jun 24.

Department of Pharmacy, The First Hospital of China Medical University, Nanjing Street No.155, Heping District, Shenyang City 110001, Liaoning, China. Electronic address:

ZCY-15, N-(3,5-dimethyladamatan-1-yl)-N-(3-methylphenyl) urea, is a candidate compound synthesized from the memantine structure and has been shown to be remarkably effective in treating Alzheimer's disease. To elucidate the pharmacokinetics and tissue distribution of ZCY-15 in rats after oral and intravenous administration, a rapid and selective LC-MS/MS method was established for the determination of ZCY-15 in rat plasma and tissues. According to the dissolution characteristics, the plasma samples were prepared by acetonitrile protein precipitation and carbamazepine was selected as the internal standard (IS). After separation by gradient elution using Aqela Venusil ASB C (2.1 × 50 mm, 3 µm), the pretreated samples were analyzed in MRM mode in positive ESI mode. The effective detection limit of this method was 1.95-1000 ng·mL. Tissue samples were collected from the heart, liver, spleen, lung, kidney, fat, muscle, brain, hippocampus, testicles or ovaries, large intestine, small intestine and stomach. The proposed method demonstrated fine precision and accuracy for analyzing ZCY-15 in selected tissues within the concentration range of standard liquid chromatography-tandem mass spectrometry. The whole analysis time was 3.6 min per sample. After oral administration, the blood and tissue concentrations of ZCY-15 in female rats were significantly higher than those in male rats. The clearance rate of ZCY-15 in female rats was lower than that in male rats. The results confirmed that there were gender differences. It has been shown that ZCY-15 could pass through the blood-brain barrier and was highly concentrated in the hippocampus. We established the first bioanalytical method to quantify ZCY-15 in rodent bio-samples for ongoing pharmacokinetic and tissue distribution studies, and the results were expected to lay foundation for the subsequent studies.
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http://dx.doi.org/10.1016/j.ejps.2021.105917DOI Listing
September 2021

Novel tigecycline resistance mechanisms in mediated by mutations in , and .

Emerg Microbes Infect 2021 Dec;10(1):1404-1417

Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

is an important pathogen in hospital acquired infections. Although tigecycline currently remains a potent antibiotic for treating infections caused by multidrug resistant (MDRAB) strains, reports of tigecycline resistant isolates have substantially increased. The resistance mechanisms to tigecycline in are far more complicated and diverse than what has been described in the literature so far. Here, we characterize -selected MDRAB strains obtained by increasing concentrations of tigecycline. We have identified mutations in , and that result in reduced susceptibility to tigecycline. Using complementation experiments, we confirm that mutations in , , and two types of mutations in correlate with tigecycline resistance. By Western blot and polysome profile analysis, we demonstrate that the mutation results in decreased expression of RRF, which affects the process of ribosome recycling ultimately leading to increased tigecycline tolerance. A transcriptional analysis shows that the mutated gene plays a role in regulating the expression of the SAM-dependent methyltransferase () and transcriptional regulators, to confer moderate tigecycline resistance. This study provides direct evidence that mutations in the , and are associated with tigecycline resistance in .
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http://dx.doi.org/10.1080/22221751.2021.1948804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274536PMC
December 2021

Immune checkpoint inhibitors combined with chemotherapy/bevacizumab therapy for patients with advanced lung cancer and heavily treated with EGFR mutation: a retrospective analysis.

J Thorac Dis 2021 May;13(5):2959-2967

Department of Medical Oncology, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, China.

Background: EGFR-mutated lung cancer poorly responded to anti-programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) monotherapy. Whether patients with EGFR-mutated lung cancer can benefit from anti-PD-1/PD-L1 therapy combined with other drugs remains controversial. We retrospectively evaluated the safety and efficacy of the PD-1 inhibitor combined with other drugs (chemotherapy and/or bevacizumab) in patients with EGFR-mutated lung cancer, who have progressed on EGFR-TKI treatment to determine the activity of the anti-PD-1/PD-L1 therapy combined with chemotherapy or/and bevacizumab therapy in heavily treated patients with EGFR-mutated lung cancer.

Methods: We identified 56 patients with EGFR-mutated lung cancer treated with PD-1/PD-L1 inhibitors alone or combined with the chemotherapy/bevacizumab therapy. The objective response rates were assessed using RECIST v1.1. Adverse events (AEs) were graded in accordance with the National Cancer Institute Common Terminology Criteria for Adverse Events v4.0. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by the Academic Ethics Committee of Jiangsu Cancer Hospital. (NO. 2019 160), and individual consent for this retrospective analysis was waived.

Results: Objective responses were observed in 6 of 56 (10.7%) patients, and the disease control rate was 53.6% (30/56). The median progression-free survival (PFS) was 3.33 months with 95% CI of 1.58-5.08 months. No patient achieved a complete response. All six patients that achieved PR were treated with the PD-1 inhibitor combined with chemotherapy or bevacizumab therapy. Three of the six patients who achieved PR were treated with radiotherapy combined with PD-1 inhibitor-based therapy. Patients treated with the PD-1 inhibitor-based therapy as second-line therapy showed relatively longer PFS and higher objective response rates than those treated with PD-1 inhibitor-based therapy as third- or late-line therapy (PFS: 5.50 3.27 months, P=0.301; objective response rates: 25.0% 6.82%, P=0.071). No additional AE profile was observed.

Conclusions: The PD-1 inhibitor combined with the chemotherapy/bevacizumab therapy showed acceptable toxicity profile and moderate efficacy on heavily treated advanced EGFR-mutated lung cancer after the exhaustion of target therapy.
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http://dx.doi.org/10.21037/jtd-20-3520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182551PMC
May 2021

Characteristics of progestin-insensitive early stage endometrial cancer and atypical hyperplasia patients receiving second-line fertility-sparing treatment.

J Gynecol Oncol 2021 Jul;32(4):e57

Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

Objective: This study investigated the characteristics of progestin-insensitive endometrioid endometrial cancer (EEC) and atypical endometrial hyperplasia (AEH) patients receiving fertility-sparing treatments and assessed the therapeutic effects of second-line fertility-preserving treatments.

Methods: Three hundred and thirty-eight patients with EEC (n=75) or AEH (n=263) receiving fertility-preserving treatment were retrospectively analyzed. 'Progestin-insensitive' was defined as meeting one of the following criteria: 1) presented with progressed disease at any time during conservative treatment, 2) remained with stable disease after 7 months of treatment, and/or 3) did not achieve complete response (CR) after 10 months of treatment. Clinical characteristics and treatment results of progestin-insensitive patients receiving second-line treatment and those of progestin-sensitive patients were compared.

Results: Eight-two patients (59 AEH and 23 EEC) were defined as progestin-insensitive and 256 as progestin-sensitive. In multivariate analysis, body mass index ≥28.0 kg/m² (odds ratio [OR]=1.898) and lesion size >2 cm (OR=2.077) were independent predictors of progestin-insensitive status. Compared to AEH patients, progestin-insensitive EEC patients had poorer second-line treatment responses (28-week cumulative CR rate after changing second-line treatment, 56.3% vs. 85.4%, p=0.011). No statistical difference was found in CR rate among different second-line treatments.

Conclusion: Obesity and larger lesion size were independent risk factors associated with progestin-insensitive status. In progestin-insensitive patients receiving second-line treatment, EEC patients had lower CR rate comparing with AEH patients. Further study with larger sample size is needed to evaluate efficacy of different second-line treatments for progestin insensitive patients.
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http://dx.doi.org/10.3802/jgo.2021.32.e57DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192233PMC
July 2021

Bioinformatic analysis of differentially expressed genes as prognostic markers in pheochromocytoma and paraganglioma tumors.

Genes Genet Syst 2021 Jul 26;96(2):55-69. Epub 2021 May 26.

Department of Hand and Microsurgery, Xiangya Hospital, Central South University.

The pathogenesis of pheochromocytoma and paraganglioma (PCPG) catecholamine-producing tumors is exceedingly complicated. Here, we sought to identify important genes affecting the prognosis and survival rate of patients suffering from PCPG. We analyzed 95 samples obtained from two microarray data series, GSE19422 and GSE60459, from the Gene Expression Omnibus (GEO) repository. First, differentially expressed genes (DEGs) were identified by comparing 87 PCPG tumor samples and eight normal adrenal tissue samples using R language. The GEO2R tool and Venn diagram software were applied to the Database for Annotation, Visualization and Integrated Discovery (DAVID) to analyze Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Gene Ontology (GO). We further employed Cytoscape with the Molecular Complex Detection (MCODE) tool to make protein-protein interactions visible for the Search Tool for Retrieval of Interacting Genes (STRING). These procedures resulted in 30 candidate DEGs, which were subjected to Kaplan-Meier analysis and validated by Gene Expression Profiling Interactive Analysis (GEPIA) to determine their influence on overall survival rate. Finally, we identified ALDH3A2 and AKR1B1, two genes in the glycerolipid metabolism pathway, as being particularly enriched in PCPG tumors and correlated with T and B tumor-infiltrating immune cells. Our results suggest that these two DEGs are closely associated with the prognosis of malignant PCPG tumors.
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http://dx.doi.org/10.1266/ggs.20-00057DOI Listing
July 2021

Saponins from ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-κB and TGF-β1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling.

J Ginseng Res 2021 May 9;45(3):408-419. Epub 2020 Sep 9.

College of Medical Science, China Three Gorges University, Yichang, China.

Background: The decreased renal function is known to be associated with biological aging, of which the main pathological features are chronic inflammation and renal interstitial fibrosis. In previous studies, we reported that total saponins from (SPJs) can availably protect acute myocardial ischemia. We proposed that SPJs might have similar protective effects for aging-associated renal interstitial fibrosis. Thus, in the present study, we evaluated the overall effect of SPJs on renal fibrosis.

Methods: Sprague-Dawley (SD) aging rats were given SPJs by gavage beginning from 18 months old, at 10 mg/kg/d and 60 mg/kg/d, up to 24 months old. After the experiment, changes in morphology, function and fibrosis of their kidneys were detected. The levels of serum uric acid (UA), β2-microglobulin (β2-MG) and cystatin C (Cys C) were assayed with ELISA kits. The levels of extracellular matrix (ECM), matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), inflammatory factors and changes of oxidative stress parameters were examined.

Results: After SPJs treatment, SD rats showed significantly histopathological changes in kidneys accompanied by decreased renal fibrosis and increased renal function; As compared with those in 3-month group, the levels of serum UA, Cys C and β2-MG in 24-month group were significantly increased ( < 0.05). Compared with those in the 24-month group, the levels of serum UA, Cys C and β2-MG in the SPJ-H group were significantly decreased. While ECM was reduced and the levels of MMP-2 and MMP-9 were increased, the levels of TIMP-1, TIMP-2 and transforming growth factor-β1 (TGF-β1)/Smad signaling were decreased; the expression level of phosphorylated nuclear factor kappa-B (NF-κB) was down-regulated with reduced inflammatory factors; meanwhile, the expression of nuclear factor erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE) signaling was aggrandized.

Conclusion: These results suggest that SPJs treatment can improve age-associated renal fibrosis by inhibiting TGF-β1/Smad, NFκB signaling pathways and activating Nrf2-ARE signaling pathways and that SPJs can be a potentially valuable anti-renal fibrosis drug.
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http://dx.doi.org/10.1016/j.jgr.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134850PMC
May 2021

Particle number emissions from light-duty gasoline vehicles in Beijing, China.

Sci Total Environ 2021 Jun 6;773:145663. Epub 2021 Feb 6.

State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China. Electronic address:

Humans are more likely to be exposed to ultrafine particles (UFPs) emitted by light-duty gasoline vehicles (LDGVs) in urban road traffic, which can cause serious bodily harm. In this study, we conducted on-road measurement of the Particle Number (PN) emissions from 18 China-3, China-4, and China-5 LDGVs on representative roads in Beijing. To clarify the impact of key parameters (standards, driving conditions, and technology) on the PN emissions, we conducted a comprehensive analysis of the emission results. We found the PN emission factors (EFs) of port fuel injection (PFI) vehicles have declined considerably under stricter standards. Furthermore, we found the average EF of China-5 LDGVs with gasoline direct injection (GDI) was 10 times that of PFI vehicles, and the PN EFs of PFI vehicles increase as the age of the engine increases (R = 0.59). In different operating conditions, the PN EFs of all test vehicles under highway driving conditions were lower than those under non-highway driving conditions (6.5%-82.0%). The PN EFs of PFI LDGVs going uphill are 1.4-2.8 times those when going downhill. The PN EFs of LDGVs under start-up were 18-47% higher than under hot-running. PN emissions were high under positive engine power and increased with vehicle specific power. The dilution ratio has a significant impact on the test results, especially in a higher vehicle specific power interval, indicating that a high dilution ratio may lead to deviation of test values. Further research needs to determine the optimal dilution ratio to minimize test deviation. This study provides important data support for PN emission control. The optimal upper limit of the primary dilution ratio should be further studied and specified as a standard.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145663DOI Listing
June 2021

Harnessing Colloidal Self-Assembled Patterns (cSAPs) to Regulate Bacterial and Human Stem Cell Response at Biointerfaces and .

ACS Appl Mater Interfaces 2021 May 29;13(18):20982-20994. Epub 2021 Apr 29.

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China.

The generation of complex physicochemical signals on the surface of biomedical materials is still challenging despite the fact that a broad range of surface modification methods have been developed over the last few decades. Colloidal self-assembled patterns (cSAPs) are combinations of unique colloids differing in size and surface chemistry acting as building blocks that can be programmed to generate surface patterns with exquisite control of complexity. This study reports on producing a variety of pre-modified colloids for the fabrication of cSAPs as well as post-assembly modifications to yield complex surfaces. The surface of cSAPs presents hierarchical micro- and nanostructures, localized hydrophilic/hydrophobic characteristics, and tunable surface functionality imparted by the individual colloids. The selected cSAPs can control bacterial adhesion (, , and ) and affect the cell cycle of human bone marrow stem cells (hBMSCs). Moreover, in a mouse subcutaneous model, cSAPs with selective [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium (SBMA) modification can reduce the inflammatory response after being challenged with bacteria. This study reveals that functionalized cSAPs are versatile tools for controlling cellular responses at biointerfaces, which is instructive for biomaterials or biodevices.
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http://dx.doi.org/10.1021/acsami.1c02591DOI Listing
May 2021

Exposure to PM2.5 Enhances the PI3K/AKT Signaling and Malignancy of ERα Expression-dependent Non-small Cell Lung Carcinoma.

Biomed Environ Sci 2021 Apr;34(4):319-323

Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.

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http://dx.doi.org/10.3967/bes2021.041DOI Listing
April 2021

Reliability and validity of the Brief Assessment of Impaired Cognition (Chinese version) for stroke patients.

Appl Neuropsychol Adult 2021 Apr 20:1-7. Epub 2021 Apr 20.

Department of Rehabilitation Medicine, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Objective and accurate cognitive assessment scales are essential for guiding cognitive rehabilitation following stroke. The aim of this study was to evaluate the validity and reliability of the Mandarin Chinese version of the Brief Assessment of Impaired Cognition (BASIC) in stroke patients. The English version of the BASIC scale was translated into Mandarin Chinese, and 56 stroke patients at a stroke treatment center were enrolled in the study. The Mini-Mental State Examination (MMSE) and BASIC scale were used to evaluate the patients' cognitive function, and content validity, structural validity, concurrent validity, internal consistency, interrater consistency and reliability and test-retest reliability were used to evaluate the test results. The correlation coefficients between each item of the BASIC scale and the total score were between 0.416 and 0.804 ( < 0.05). The total score on the BASIC scale was correlated with the total score on the MMSE ( = 0.479,  < 0.05). Four factors were extracted by exploratory factor analysis, and the cumulative variance contribution rate was 74.932%. The factor loading of each item on the corresponding factor was > 0.5, indicating that the scale has good structural validity. Internal consistency was good (Cronbach's  = 0.821), as were interrater (ICC > 0.95) and test-retest reliability (ICC = 0.815-0.941). Therefore, the Chinese version of the BASIC scale has good reliability and validity and can assist in screening for cognitive dysfunction or dementia in stroke patients.
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http://dx.doi.org/10.1080/23279095.2021.1908287DOI Listing
April 2021

[Difference of musculoskeletal ultrasound imaging of focus of knee joint tendon between patients with knee osteoarthritis and healthy subjects].

Zhongguo Zhen Jiu 2021 Mar;41(3):303-6

College of Acupuncture-Moxibustion and Tuina, Liaoning University of TCM, Shenyang 110847, China.

Objective: To observe the imaging features of focus of knee joint tendon in patients with knee osteoarthritis (KOA) by musculoskeletal ultrasound (MSUS) technique.

Methods: One hundred KOA patients and 100 healthy subjects were included. All the KOA patients were palpated by the sequence of foot meridian, foot meridian, foot meridian and foot three -meridians, and the focus of knee tendon and its frequency were recorded by MSUS technique. The top-5 focus of knee tendon of KOA patients and the corresponding focus of knee tendon of healthy subjects were selected as the observation sites to compare the differences of thickness of ligament and tendon soft tissue in the extension and flexion positions of the knee joint.

Results: The top-5 focus of knee tendon of KOA patients were located in medial inferior patella, medial tibial condyle, inferior patella, Zusanlici and Hedingci. The thickness of ligaments and tendons in extension and flexion positions in KOA patients were thicker than that in healthy subjects (<0.05).

Conclusion: The focus of knee joint tendon in KOA patients shows significantly thickened musculoskeletal imaging features.
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http://dx.doi.org/10.13703/j.0255-2930.20200317-k0001DOI Listing
March 2021

Substrate degradation, biodiesel production, and microbial community of two electro-fermentation systems on treating oleaginous microalgae Nannochloropsis sp.

Bioresour Technol 2021 Jun 6;329:124932. Epub 2021 Mar 6.

College of Power and Energy Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address:

Electro-fermentation system (EFS) emerges its effectiveness on treating microalgae for biodiesel production, but much is unknown about biodegradation behaviors, biodiesel characteristics, and microbial community. Compared with conventional fermentation system (CFS), microbial electrolysis cell-based EFS (MEC-EFS) and microbial fuel cell-based EFS (MFC-EFS) were investigated for the performance while treating microalgae Nannochloropsis sp. Results indicated that MEC-EFS presented much higher first-order decomposition rate coefficients of carbohydrates and proteins (1.212/d and 0.951/d) than those of CFS (0.615/d and 0.794/d) and MFC-EFS (0.518/d and 0.415/d). Compared with MFC-EFS, MEC-EFS showed better electrochemical performance (2.17 A/mvs. 0.95 A/m). Moreover, MEC-EFS reached the highest extracted lipid to biomass ratio (43.3%), followed by MFC-EFS (32.3%) and CFS (27.7%). By strengthened microbial biohydrogenation, MEC-EFS and MFC-EFS had higher saturated fatty acids ratio (78.8% and 70.6%) than that of CFS (56.1%). For MEC-EFS, enriched Ruminococcus and Geobacter in anodic biofilm might contribute to favorable biohydrogenation and electrochemical performance.
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http://dx.doi.org/10.1016/j.biortech.2021.124932DOI Listing
June 2021

Downregulation of hsa_circ_0006220 and its correlation with clinicopathological factors in human breast cancer.

Gland Surg 2021 Feb;10(2):816-825

Department of Geriatric Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China.

Background: Circular ribonucleic acids (circRNAs) are highly stable and conserved forms of RNAs present in all eukaryotes. They can modulate the expression of genes by sponging specific micro RNAs (miRNAs), thereby affecting various disease processes. However, their expression pattern in human breast cancer has not been elucidated.

Methods: In this study, differentially expressed circRNAs in breast cancer tissues and paired noncancerous tissues were analyzed using an Arraystar Human circRNA Microarray, and hsa_circ_0006220 was selected for its 27-fold downregulation in breast cancer tissues. Its expression was also verified in 50 breast cancer and paired noncancerous tissues using real-time polymerase chain reaction (RT-PCR). An analysis of the expression of hsa_circ_0006220 and the clinicopathological factors in breast cancer was conducted. A receiver operating characteristic (ROC) curve of hsa_circ_0006220 was constructed. The interaction between hsa_circ_0006220 and five possible target miRNAs was predicted, and their expression were verified when overexpressing hsa_circ_0006220 by RT-PCR.

Results: Hsa_circ_0006220 was found to be significantly downregulated in breast cancer tissues compared to the paired noncancerous tissues by microarray and RT-PCR. The expression of hsa_circ_0006220 was significantly inversely correlated with histological type (P=0.0028) and lymph node metastasis (P=0.0341). The area under the ROC curve (AUC) was 0.706. Five miRNAs that might be sponged by hsa_circ_0006220 were predicted. MiR-197-5p was significantly downregulated after overexpression of hsa_circ_0006220.

Conclusions: Our results indicated that hsa_circ_0006220 may play a role in human breast cancer and might be a potential tumor marker for breast cancer screening.
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http://dx.doi.org/10.21037/gs-21-42DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944078PMC
February 2021

Genome-Scale Phylogenetic and Population Genetic Studies Provide Insight Into Introgression and Adaptive Evolution of Species in East Asia.

Front Genet 2021 22;12:625600. Epub 2021 Feb 22.

Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

As a typical marine adaptive radiation species, most species are widely distributed in East Asian offshore, which have diversified morphological characteristics and different ecological habits. The phylogenetic relationship and population structure of the species was complicated because of incomplete lineage sorting, widespread hybridization and introgression. Therefore, to systematically clarify the phylogenetic relationships of genus, explore the introgression and natural hybridization between different species, and detect the selective signatures in the adaptive evolution of diversified traits, whole-genome resequencing was used in 122 samples from 10 species. Phylogenetic analysis showed solid sister-group relationships between and , , and , , and , , and . Further admixture analysis indicated the divergence of population and the bidirectional gene flow between and . Using species-specific homozygous genetic variance sites, we detected the asymmetric introgression between and at China East sea and southern Taiwan Strait. By genome-scale genetic diversity scanning, we detected two copies of , and related to the semilunar reproduction rhythm in , involved in memory formation, embryo maturation and female reproduction. Furthermore, we also found lots of specific mutations in CDS region of circadian rhythm related genes and endocrine hormone genes. For species, our research provides reliable genetic resources and results for the phylogeny, introgression, hybridization and adaptive evolution, and could be used as a guide for the formulation of the protection and proliferation release policies.
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http://dx.doi.org/10.3389/fgene.2021.625600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937929PMC
February 2021

Molecular cytogenetic characterization and fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids.

Mol Cytogenet 2021 Mar 6;14(1):15. Epub 2021 Mar 6.

Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.

Background: Partial amphiploids created by crossing octoploid tritelytrigia(2n = 8× = 56, AABBDDEE) and Thinopyrum intermedium (2n = 6× = 42, StStJJJJ) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH.

Results: The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia (2n = 8× = 56, AABBDDEE) but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20% of PPS. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively.

Conclusions: New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.
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http://dx.doi.org/10.1186/s13039-021-00536-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937273PMC
March 2021

The Diagnosis and Management of Allergic Reactions Caused by Chinese Materia Medica.

Clin Rev Allergy Immunol 2021 Feb 19. Epub 2021 Feb 19.

Asthma and Allergic Diseases Center, University of Virginia, Charlottesville, VA, USA.

Traditional Chinese medicines (TCM) have been used in China for thousands of years. Although TCM has been generally perceived to be safe, adverse reactions to Chinese materia medica (CMM) have been reported. Most of the adverse reactions are allergic in nature, but other mechanisms may play a role. This review focuses on the mechanism and clinical presentation of these allergic reactions. Allergic reactions can occur as a result of the active and inactive ingredients of CMM. Impurities and chemicals generated during the production process can also lead to allergic or adverse reactions. Environmental factors such as temperature, humidity, and light can cause changes in the allergenicity of drugs. Human error in formulating CMM drugs also contributes to adverse drug reactions. The management of allergic reactions to CMM includes taking a good history, avoidance of medications in the same class as those which caused prior reactions, the proper training of staff, adherence to manufacturer guidelines and expiration dates, evaluation of benefit and risk balance, and the formulation of a risk management strategy for the use of CMM. A small test dose of a considered drug before using, improvements in drug purification technology, and proper storage and clinical administration help reduce allergic reactions due to CMM.
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http://dx.doi.org/10.1007/s12016-020-08812-7DOI Listing
February 2021

Theoretical Screening and Experimental Synthesis of Ultrahigh-Iodine Capture Covalent Organic Frameworks.

ACS Appl Mater Interfaces 2021 Mar 18;13(8):10513-10523. Epub 2021 Feb 18.

College of Chemistry, Jilin University, Changchun, Jilin 130012, China.

Radioactive materials of nuclear waste would be hazardous to human health such as the reproductive and metabolic system. How to design a radioactive material adsorbent quickly and efficiently is still a great challenge. In this study, a strategy for the efficient design of a high-potential radioactive iodine uptake adsorbent by theoretical screening is proposed. The following experiments which use covalent organic frameworks (COFs) as demonstration have great agreement with the theoretical screening prediction. Three screened COFs show ultrahigh iodine adsorption, which reaches up to 6.4 g/g (640% in mass) in vapor and 99.9 mg/g in solution, owing to the pore size and the functional groups in COFs.
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http://dx.doi.org/10.1021/acsami.0c17748DOI Listing
March 2021

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products.

Pol J Microbiol 2020 Sep 8;69(3):339-347. Epub 2020 Sep 8.

College of Grassland Science, Shanxi Agricultural University, Taigu, China.

Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of BY5, LN2, LN4, and CL32 have been assayed using alkaline-lignin as the unique carbon source. Further, the Lip (lignin peroxidase) and Mnp (manganese peroxidase) activities of strains were investigated. Lip activity of LN4 was highest after 72 h of incubation and reached 7151.7 U · l. Mnp activity of CL32 was highest after 48 h and reached 12533 U · l. The analysis of alkaline-lignin degradation products by GC-MS revealed that the strains screened could utilize aromatic esters compounds such as dibutyl phthalate (DBP), and decomposite monocyclic aromatic compounds through the DBP aerobic metabolic pathway. The results indicate that BY5, LN2, LN4, and CL32 have high potential to degrade alkaline-lignin, and might utilize aromatic compounds by DBP aerobic metabolic pathway in the process of lignin degradation.
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http://dx.doi.org/10.33073/pjm-2020-037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810122PMC
September 2020

Predictive value of baseline F-FDG PET/CT and interim treatment response for the prognosis of patients with diffuse large B-cell lymphoma receiving R-CHOP chemotherapy.

Oncol Lett 2021 Feb 18;21(2):132. Epub 2020 Dec 18.

The First School of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.

The present study aimed to investigate the prognostic value of baseline F-FDG PET/CT quantitative parameters and interim treatment response, and to assess whether the combination of these could improve the predictive efficacy in patients with diffuse large B-cell lymphoma (DLBCL) receiving R-CHOP chemotherapy. PET/CT images and clinical data of 64 patients with DLBCL who had undergone F-FDG PET/CT scan before and after 3 or 4 cycles of R-CHOP chemotherapy were retrospectively reviewed. The quantitative parameters including standardized uptake value (SUV), metabolic tumor volume (MTV), total lesion glycolysis (TLG), and maximum diameter of the maximum lesion (Dmax) were measured on baseline PET/CT images. Cox proportional hazards model was used to evaluate the influence of baseline PET/CT parameters, clinical indicators and interim treatment response on prognosis. Survival analysis was performed using Kaplan-Meier method. Receiver operating characteristic (ROC) curve analysis was performed to estimate the predictive efficacy of the combination of baseline PET/CT parameters and interim treatment response. Ann Arbor stage, International Prognostic Index (IPI), lactate dehydrogenase (LDH), necrosis, MTVmax, TLGmax, Dmax and interim treatment response showed association with 2-year progression-free survival (PFS, P<0.05). LDH, necrosis, MTVmax, MTVsum, TLGmax, TLGsum, Dmax and interim treatment response showed association with 2-year overall survival (OS, P<0.05). Ann Arbor stage, Dmax and interim treatment response were found to be independent predictors of 2-year PFS (P<0.05), while Dmax and interim treatment response were found to be independent predictors of 2-year OS (P<0.05). The PFS and OS curves of Dmax <5.7 cm group and Dmax ≥5.7 cm group, complete response (CR) group and non-CR group were significantly different, respectively (P<0.05). The baseline F-FDG PET/CT parameters and interim treatment response have important prognostic values in DLBCL patients who received R-CHOP chemotherapy. Combined application of Dmax and interim treatment response improved the predictive efficacy of 2-year PFS. It may be helpful to identify patients who are at high-risk of relapse and to guide early clinical intervention of these patients.
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http://dx.doi.org/10.3892/ol.2020.12393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798034PMC
February 2021

Population-based outcome of muscle-invasive bladder cancer following radical cystectomy: who can benefit from adjuvant chemotherapy?

Transl Androl Urol 2021 Jan;10(1):356-373

Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: The benefit of adjuvant chemotherapy remains controversial in muscle-invasive bladder cancer (MIBC) after radical cystectomy. The present study's primary objective was to construct a predictive tool for the reasonable application of adjuvant chemotherapy.

Methods: All of the patients analyzed in the present study were recruited from the Surveillance Epidemiology and End Results program between 2004 and 2015. Propensity score matching (PSM) was used to reduce inherent selection bias. Cox proportional hazards models were applied to identify the independent prognostic factors of overall survival (OS) and cancer-specific survival (CSS), which were further used to construct prognostic nomogram and risk stratification systems to predict survival outcomes. The prognostic nomogram's performance was assessed by concordance index (C-index), receiver-operating characteristic (ROC) and calibration curves. Decision curve analysis (DCA) was performed to evaluate the clinical net benefit of the prognostic nomogram.

Results: A total of 6,384 patients with or without adjuvant chemotherapy were included after PSM. Several independent predictors for OS and CSS were identified and further applied to establish a nomogram for 3-, 5- and 10-year, respectively. The nomogram showed favorable discriminative ability for the prediction of OS and CSS, with a C-index of 0.709 [95% confidence interval (CI): 0.699-0.719] for OS and 0.728 (95% CI: 0.718-0.738) for CSS. ROC and calibration curves showed satisfactory consistency. The DCA revealed high clinical positive net benefits of the prognostic nomogram. The different risk stratification systems showed that adjuvant chemotherapy resulted in better OS (P<0.001) and CSS (P<0.001) than without adjuvant chemotherapy for high-risk patients; while the OS (P=0.350) and CSS (P=0.260) for low-risk patients were comparable.

Conclusions: We have constructed a predictive model and different risk stratifications for selecting a population that could benefit from postoperative adjuvant chemotherapy. Adjuvant chemotherapy was found to be beneficial for high-risk patients, while low-risk patients should be carefully monitored.
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http://dx.doi.org/10.21037/tau-20-960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844522PMC
January 2021

Network Pharmacology-Based Analysis on the Mechanism of Action of Ephedrae Herba-Cinnamomi Ramulus Couplet Medicines in the Treatment for Psoriasis.

Med Sci Monit 2021 Jan 29;27:e927421. Epub 2021 Jan 29.

Department of Dermatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland).

BACKGROUND This study explored the mechanism of action of Ephedrae Herba-Cinnamomi Ramulus couplet medicine (MGCM) at the pharmacological level in the treatment of psoriasis. MATERIAL AND METHODS The active ingredients in MGCM were mined through literature retrieval and the BATMAN-TCM database, and potential targets were predicted. In addition, targets associated with psoriasis were acquired using multiple disease-related databases. Thereafter, an interaction network between candidate MGCM targets and the known psoriasis-associated targets was constructed based on the protein-protein interaction (PPI) data, using the STRING database. Then, the topological parameter degree was determined for mining the core targets for MGCM in the treatment of psoriasis, which also represented the major hubs within the PPI network. In addition, the core networks of targets and ingredients were constructed using Cytoscape software to apply MGCM in the treatment for psoriasis. These core targets were then analyzed for Gene Ontology biological processes and Kyoto Encyclopedia of Genes and Genomes pathway enrichment using OmicShare. RESULTS The ingredient-target core network of MGCM for treating psoriasis was constructed; it contained 52 active ingredients and corresponded to 19 core targets. In addition, based on enrichment analysis, these core targets were majorly enriched for several biological processes (immuno-inflammatory responses, leukocyte differentiation, energy metabolism, angiogenesis, and programmed cell death) together with the relevant pathways (Janus kinase-signal transducer and activator of transcription, toll-like receptors, nuclear factor kappaB, vascular endothelial growth factor, and peroxisome proliferator-activated receptor), thus identifying the possible mechanism of action of MGCM in treating psoriasis. CONCLUSIONS The present network pharmacology study indicated that MGCM alleviates various pathological factors of psoriasis through multiple compounds, multiple targets, and multiple pathways.
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http://dx.doi.org/10.12659/MSM.927421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852043PMC
January 2021

Endothelial cell-activating antibodies in COVID-19.

medRxiv 2021 Jan 20. Epub 2021 Jan 20.

Patients with coronavirus disease 19 ( ) are at high risk for fibrin-based occlusion of vascular beds of all sizes. Although endothelial cell activation has regularly been described as part of the COVID-19 thrombo-inflammatory storm, the upstream mediators of this activation have yet to be fully elucidated. Here, we began by pursuing the hypothesis that circulating factors such as neutrophil extracellular trap (NET) remnants, D-dimer, or C-reactive protein might predict the COVID-19 serum samples (n=118) that most robustly activated cultured endothelial cells. Indeed, we found modest correlations between serum NET remnants (cell-free DNA, myeloperoxidase-DNA complexes, citrullinated histone H3) and upregulation of surface E-selectin, VCAM-1, and ICAM-1 on endothelial cells. However, a more robust predictor of the ability of COVID-19 serum to activate endothelial cells was the presence of circulating antiphospholipid antibodies, specifically anticardiolipin IgG and IgM and anti-phosphatidlyserine/prothrombin (anti-PS/PT) IgG and IgM. Depletion of total IgG from anticardiolipin-high and anti-PS/PT-high samples markedly restrained upregulation of E-selectin, VCAM-1, and ICAM-1. At the same time, supplementation of control serum with patient IgG was sufficient to trigger endothelial cell activation. These data are the first to reveal that patient antibodies are a driver of endothelial cell activation and add important context regarding thrombo-inflammatory effects of COVID-19 autoantibodies in severe COVID-19.
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http://dx.doi.org/10.1101/2021.01.18.21250041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836141PMC
January 2021

An Off-Policy Trust Region Policy Optimization Method With Monotonic Improvement Guarantee for Deep Reinforcement Learning.

IEEE Trans Neural Netw Learn Syst 2021 Jan 22;PP. Epub 2021 Jan 22.

In deep reinforcement learning, off-policy data help reduce on-policy interaction with the environment, and the trust region policy optimization (TRPO) method is efficient to stabilize the policy optimization procedure. In this article, we propose an off-policy TRPO method, off-policy TRPO, which exploits both on- and off-policy data and guarantees the monotonic improvement of policies. A surrogate objective function is developed to use both on- and off-policy data and keep the monotonic improvement of policies. We then optimize this surrogate objective function by approximately solving a constrained optimization problem under arbitrary parameterization and finite samples. We conduct experiments on representative continuous control tasks from OpenAI Gym and MuJoCo. The results show that the proposed off-policy TRPO achieves better performance in the majority of continuous control tasks compared with other trust region policy-based methods using off-policy data.
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http://dx.doi.org/10.1109/TNNLS.2020.3044196DOI Listing
January 2021

Two-dimensional ultrathin surfactant-encapsulating polyoxometalate assemblies as carriers for monodispersing noble-metal nanoparticles with high catalytic activity and stability.

Dalton Trans 2021 Feb;50(5):1666-1671

Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P. R. China.

Noble metal nanoparticles (NMNPs) with excellent catalytic activity and stability play an important role in the field of environmental governance. A uniform distribution and a strong binding force with the carriers of the noble metal nanoparticles are important, but avoidance of the use of additional reducing agents is a promising direction of research. Herein, 2D ultrathin surfactant-encapsulating polyoxometalate (SEP) nanosheets constructed by the self-assembly of dodecyldimethylammonium bromide (DODA) and molybdophosphate (H3PMo12O40, PMo12) are designed to be versatile carriers for Ag nanoparticles. Under the synergistic effect of the well-arranged PMo12 units, encapsulating hydrophobic oleic acid (OA) and reductive molybdophosphate under Xe lamp irradiation, the silver oleate (AgOA)-derived Ag nanoparticles (5 ± 2 nm) are monodispersed on the DODA-PMo12 assemblies and form the Agx/DODA-PMo12 composite. The optimized Ag4.89/DODA-PMo12 composite exhibits high catalytic activity and stability in the degradation of 4-nitrophenol (4-NP), which reaches a superior rate constant of 6.49 × 10-3 s-1 and without significant deterioration after three recycles. This technique can be facilely promoted to other noble metal nanoparticles with excellent catalytic activity and stability.
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http://dx.doi.org/10.1039/d0dt03976dDOI Listing
February 2021

Decoration of Material Surfaces with Complex Physicochemical Signals for Biointerface Applications.

ACS Biomater Sci Eng 2020 04 20;6(4):1836-1851. Epub 2020 Mar 20.

Centre for Human Tissue & Organ Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangzhou 518055, China.

The surface properties of biomaterials are crucial at controlling biological interactions. Cells or tissues sense different stimuli from surfaces and respond accordingly. A number of studies have reported that fabricating complex stimuli-presenting surfaces is beneficial for mimicking and understanding the in vivo scenario where multi-physicochemical cues are present. Biological responses toward these surfaces could be either negative, such as immune responses, or positive, such as tissue regeneration. An ideal material surface should, therefore, be multifunctional, triggering the desired biological process and suppressing the unwanted side effects. The methods for material surface decoration can be very sophisticated, depending on the applications such as biosensors, medical devices, and/or implants. To date, decorating material surfaces with complex chemistries and topographies is still challenging, and methods are not straightforward. The majority of the methods require multiple steps and combinational approaches that include mask-based techniques, lithography, wet or dry etching, wet chemistry, or vapor-based coatings, among others. Although these methods have been established in the laboratory, easy-to-access and straightforward approaches need to be explored. This Review summarizes the state-of-the-art techniques for generating patterned multichemical and multitopographic signals on material surfaces, and the potential of having these surfaces in biointerface applications.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01806DOI Listing
April 2020

Producing and storing self-sustaining drinking water from rainwater for emergency response on isolated island.

Sci Total Environ 2021 May 7;768:144513. Epub 2021 Jan 7.

College of Defense Engineering, Peoples' Liberation Army Engineering University, Nanjing 210007, China.

Drinking water on isolated islands includes treated rainwater, water shipped from the mainland, and desalinated seawater. However, marine transportation and desalination plants are vulnerable to emergencies, such as extreme weather, making self-sustaining stand-by water for emergency response essential. Rainwater is ideal for producing the stand-by water, and rainwater harvesting is sustainable and clean, and prolonged biostability can be ensured by managing biological and chemical parameters. The present study applied a stand-by drinking water purification system (primarily including nanofiltration and low-dose chlorination) to explore the feasibility of producing and storing cleaner drinking water from rainwater and the following conclusions were drawn. First, treatment of rainwaters ensures biosafety for seven days, which is longer than that for untreated rainwater; the proportion of opportunistic pathogens decreased from 23.40-7.77% after nanofiltration, and it was proposed that the microbial community converges after advanced water treatment. Second, chemical qualities were improved. Local resource coral sand prevents pH in rainwater from decreasing below 6.5, and treated rainwater had lower disinfection by-product potential and higher disinfection efficiency, allowing periodical rainwater recycling. Third, harvesting rainwater was extremely cost-effective, with an operation cost of 1.5-2.5 RMB/m. From biosafety, chemical safety, and economic cost perspectives, self-sustaining water from rainwater can contributes to the development of sustainable and cost-effective water supply systems on isolated islands. Mixing treated rainwater and desalinated seawater reasonably guarantees sufficiency and safety.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144513DOI Listing
May 2021

The decreased expression of hsa_circ_0043278 and its relationship with clinicopathological features of breast cancer.

Gland Surg 2020 Dec;9(6):2044-2053

Department of Geriatric Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China.

Background: Breast cancer is one of the most significant causes of death in women around the world. Circular RNAs (circRNAs), which are a novel class of conserved RNA molecules, are involved in the occurrence and development of various diseases, especially malignancies; however, researchers rarely report their roles in human breast cancer.

Methods: In the present study, the differentially expressed levels of circRNAs in human breast cancer tissues and paired noncancerous tissues were screened by circRNA microarray. Hsa_circ_0043278 was downregulated 43-fold in breast cancer and was selected for further analysis. The expression of hsa_circ_0043278 was verified in breast cancer specimens and paired noncancerous tissues by quantitative reverse transcription polymerized chain reaction (qRT-PCR) technique. The relationship between the expression of hsa_circ_0043278 and the clinicopathological features was analyzed.

Results: Among the 520 differentially expressed circRNAs, 292 significantly upregulated circRNAs and 228 downregulated circRNAs in the breast cancer tissues compared with the paired noncancerous tissues. The area under the receiver operating characteristic (ROC) curve of hsa_circ_0043278 was 0.690. The results of the bioinformatics prediction showed five target miRNAs that might be sponged by hsa_circ_0043278. The expression of hsa_circ_0043278 was associated with lymph node metastasis and histological type of the patient. Patients with lymph node metastasis have tumors with significantly downregulated expression of hsa_circ_0043278 (P=0.0201).

Conclusions: Our results suggest that hsa_circ_0043278 is downregulated and may play a key role in human breast cancer.
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http://dx.doi.org/10.21037/gs-20-825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804522PMC
December 2020
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