Publications by authors named "Yue Luo"

193 Publications

IP-10 Interferes With the Antiviral Response of Direct-Acting Antiviral Agents for Hepatitis C Virus Infection.

Front Public Health 2022 18;10:911551. Epub 2022 Jul 18.

Center for Liver Diseases, Beijing Ditan Hospital, Capital Medical Univerisity, Beijing, China.

Background: Increased interferon (IFN)-gamma inducible protein-10 (IP-10) level has been shown to be associated with sustained virologic responses (SVRs) to pegylated interferon-alpha 2a/ribavirin-based therapy in patients with chronic hepatitis C (CHC). We investigated the relationship between IP-10 and treatment response in patients with CHC treated with direct-acting antiviral agents (DAAs) therapy.

Methods: We measured the dynamic changes of IP-10 in samples from 90 patients with CHC. The serum IP-10 levels, intrahepatic expressions of IP-10 mRNA, and protein were determined, respectively. For the experiments, the expression changes of IP-10 in hepatitis C virus (HCV)-replicating Huh-7 cells with or without non-structural protein 5A (NS5A) inhibitor were analyzed using real-time reverse transcription-polymerase chain reaction and Western blotting.

Results: Patients with chronic hepatitis C had increased baseline IP-10 levels, intrahepatic IP-10 mRNA, and protein expression. After initiating DAAs therapy, serum IP-10 levels decreased gradually in patients who achieved cure, whereas in patients who failed the therapy, IP-10 levels did not change significantly or recovered from the initial decline. Multivariate logistic regression analysis confirmed that baseline IP-10 level ≤ 450 pg/ml and decline >30% at 12 weeks independently predicted the SVR in patients with CHC who received DAAs. , the expression of IP-10 mRNA and protein in HCV-replicating Huh-7 cells increased significantly. However, such activities were downregulated by NS5A inhibitor, followed by the reduction of HCV RNA levels and a decline in IP-10 levels.

Conclusion: IP-10 interfered with HCV replication in hepatocytes and the dynamic decline in IP-10 levels during DAA treatment predicted the SVR in patients with CHC.
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http://dx.doi.org/10.3389/fpubh.2022.911551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342904PMC
August 2022

Imbalanced T-Cell Subsets May Facilitate the Occurrence of Osteonecrosis of the Femoral Head.

J Inflamm Res 2022 22;15:4159-4169. Epub 2022 Jul 22.

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

Background: Osteonecrosis of the femoral head (ONFH) is a complex disease resulting in degeneration of the hip joint. The pathogenesis of ONFH is largely unknown, but alterations in immunological factors have been proposed to play a role.

Methods: We included 109 patients with ONFH and 109 age-, sex-, and body mass index-matched healthy controls in this study. The percentage of circulating CD3, CD4, and CD8 lymphocytes among the total lymphocytes was identified by flow cytometry and compared between the cases and controls. Subgroup analysis within each etiological group and correlation analysis of T-cell subset levels with disease duration were performed. Furthermore, we compared the expression patterns of CD4, RANKL, and FoxP3 in the femoral head of healthy and glucocorticoid (GC)-treated ONFH rats.

Results: The results showed that CD3 and CD4 T-cell counts and the CD4/CD8 ratio were significantly higher in patients with ONFH and that CD3 lymphocyte levels were negatively correlated with disease duration. The CD4 T-cell levels and CD4/CD8 ratios in the GC-ONFH etiological group were lower than those in the idiopathic-, traumatic-, and alcoholic-ONFH groups, while the CD8 T-cell levels were higher. Furthermore, the CD3, CD4, and CD8 T-cell counts and the CD4/CD8 ratio were higher in the GC-ONFH group than in the control group. Finally, we observed diminished levels of FoxP3/CD4 double-positive T regulatory cells and increased RANKL T-cell levels in the bone marrow of the femoral head in GC-ONFH rats.

Conclusion: The imbalance of T-cell subsets might be involved in the pathophysiological process of ONFH, and diminished CD4/FoxP3 T regulatory cells may be associated with increased RANKL T cells in the bone marrow of the femoral head in GC-ONFH, which may facilitate bone resorption and collapse of the femoral head.

Trial Registration: This study was registered in the Chinese Clinical Trial Registry (Registration number: ChiCTR2100042642).
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http://dx.doi.org/10.2147/JIR.S367214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328079PMC
July 2022

Post-Translational Modifications in Atopic Dermatitis: Current Research and Clinical Relevance.

Front Cell Dev Biol 2022 7;10:942838. Epub 2022 Jul 7.

Shanghai Skin Disease Hospital of Tongji University, Shanghai, China.

Atopic dermatitis (AD) is a chronic and relapsing cutaneous disorder characterized by compromised immune system, excessive inflammation, and skin barrier disruption. Post-translational modifications (PTMs) are covalent and enzymatic modifications of proteins after their translation, which have been reported to play roles in inflammatory and allergic diseases. However, less attention has been paid to the effect of PTMs on AD. This review summarized the knowledge of six major classes (including phosphorylation, acetylation, ubiquitination, SUMOylation, glycosylation, o-glycosylation, and glycation) of PTMs in AD pathogenesis and discussed the opportunities for disease management.
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http://dx.doi.org/10.3389/fcell.2022.942838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301047PMC
July 2022

Exploring Older Adults' Nighttime Trips to the Bathroom Under Different Lighting Conditions: An Exploratory Field Study.

HERD 2022 Jul 18:19375867221113067. Epub 2022 Jul 18.

Department of Industrial and Systems Engineering, University of Florida, Gainesville, FL, USA.

Purpose: The field study was to understand older adults' reactions to and use of different low-light conditions while walking to bathrooms in the dark in their homes. Low-light conditions included participants' usual nightlights and a destination-based LED strip lighting system.

Background: Older adults encounter fall accidents while going to bathrooms at night due to low illuminance levels. They also fear falling due to previous fall histories or visual impairments. This field study tested and compared a destination-based LED strip lighting system with their usual nightlights on their movement and fear of falls.

Methods: Fifteen older adults from an independent living facility participated in the within-subject design experiment, walking under two scenarios in random order: with usual nightlights turned on or with the destination-based LED strip lighting system turned on. Body-worn sensors were used to collect participants' movement behaviors, and subjective questionnaires were used to understand participants' anxiety under the two low-light conditions. Further, semi-structured interviews were conducted to understand their nightlight usage patterns and their evaluations of the destination-based LED strip lighting system.

Results: Participants walked more smoothly under the destination-based LED strip lighting system scenario. However, the anxiety states were not statistically different between the two scenarios.

Conclusion: Visual cues in the dark can benefit older adults' safe movement. However, the application of the lab-effective LED strip lighting system in home settings should consider older adults' floor plans and their furniture layout, both indoor and outdoor ambient lighting sources, and their lifestyles.
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http://dx.doi.org/10.1177/19375867221113067DOI Listing
July 2022

Effectiveness and safety of acupuncture for postoperative ileus following gastrointestinal surgery: A systematic review and meta-analysis.

PLoS One 2022 18;17(7):e0271580. Epub 2022 Jul 18.

Acupuncture Anesthesia Clinical Research Institute, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Postoperative ileus (POI) is an important complication of gastrointestinal (GI) surgery. Acupuncture has been increasingly used in treating POI. This study aimed to assess the effectiveness and safety of acupuncture for POI following GI surgery.

Methods: Seven databases (PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, Wan fang Data, VIP Database for Chinese Technical Periodicals, and Chinese Biomedical Literature Database) and related resources were searched from inception to May 30, 2021. Randomized controlled trials (RCTs) reporting the acupuncture for POI in GI were included. The quality of RCTs was assessed by the Cochrane Collaboration Risk of Bias tool, and the certainty of the evidence was evaluated by the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach. A meta-analysis was performed by using RevMan 5.4 software.

Results: Eighteen RCTs involving 1413 participants were included. The meta-analysis showed that acupuncture could reduce the time to first flatus (TFF) (standardized mean difference [SMD] = -1.14, 95% confidence interval [CI]: -1.54 to -0.73, P < 0.00001), time to first defecation (TFD) (SMD = -1.31, 95% CI: -1.88 to -0.74, P < 0.00001), time to bowel sounds recovery (TBSR) (SMD = -1.57, 95% CI: -2.14 to -1.01, P < 0.00001), and length of hospital stay (LOS) (mean difference [MD] = -1.68, 95% CI: -2.55 to -0.80, P = 0.0002) compared with usual care. A subgroup analysis found that acupuncture at distal acupoints once daily after surgery had superior effects on reducing TFF and TFD. A sensitivity analysis supported the validity of the finding. Acupuncture also manifested an effect of reducing TFF, TFD and TBSR compared with sham acupuncture but the result was not stable. Relatively few trials have reported whether adverse events have occurred.

Conclusions: Acupuncture showed a certain effect in reducing POI following GI surgery with very low-to-moderate quality of evidence. The overall safety of acupuncture should be further validated. More high-quality, large-scale, and multicenter original trials are needed in the future.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0271580PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292096PMC
July 2022

Methylated Septin 9 as a Promising Biomarker in the Diagnosis and Recurrence Monitoring of Colorectal Cancer.

Dis Markers 2022 2;2022:7087885. Epub 2022 Jul 2.

Department of Emergency Surgery, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou 350001, China.

Purpose: The clinical utility of plasma methylated septin 9 () DNA in screening and recurrence monitoring for colorectal cancer (CRC) is highly promising. The present study was performed to determine the diagnostic value of in CRC detection and recurrence monitoring in Chinese patients.

Methods: Overall, 616 patients newly diagnosed with CRC and 122 individuals with no evidence of disease were recruited from October 1, 2019, to May 31, 2021, at Fujian Medical University Union Hospital. Plasma and serum samples were collected for analyzing , carcinoembryonic antigen (CEA), and carbohydrate antigen-19-9 (CA19-9). Data on clinicopathological characteristics were collected and analyzed. Sensitivity and specificity were calculated to evaluate the diagnostic potential of each marker; the receiver operating characteristic (ROC) curve was applied for the assessment of diagnostic value, and comparisons among , CEA, CA19-9, and their combination were assessed via ROC curves.

Results: achieved an overall sensitivity and specificity of 72.94% and 81.97%, respectively, with an area under the curve (AUC) value of 0.826, which were higher than those of CEA (sensitivity: 43.96%; specificity: 96.72%; AUC: 0.789) and CA19-9 (sensitivity: 14.99%; specificity: 96.61%; AUC: 0.590). The combination of , CEA, and CA19-9 further improved sensitivity, specificity, and AUC value (sensitivity: 78.43%; specificity: 86.07%; AUC: 0.878), respectively. Notably, the positivity rate was significantly associated with TNM stage, T stage, N stage, tumor size, vascular invasion, and nerve invasion among patients with CRC. A 100% correlation was observed between the positive results of the test and recurrence or metastasis in patients after therapeutic intervention.

Conclusion: Our findings suggest that may represent a potential biomarker for the diagnosis and prognosis of CRC compared with CEA and CA19-9. Postoperative status may represent the first noninvasive marker of CRC recurrence or metastasis.
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http://dx.doi.org/10.1155/2022/7087885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271001PMC
July 2022

Therapeutic Drug Monitoring of Ceftazidime-Avibactam Concentrations in Carbapenem-Resistant Infected Patients With Different Kidney Statuses.

Front Pharmacol 2022 22;13:780991. Epub 2022 Jun 22.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Institute of Clinical Pharmacy, Central South University, Changsha, China.

Carbapenem-resistant (CRKP) is the most common carbapenem-resistant with high mortality. Ceftazidime-avibactam (CAZ-AVI) has exhibited excellent activity against CRKP. However, the efficacy of CAZ-AVI in KPC-producing CRKP-infected patients with different kidney statuses varies, such as renal insufficiency, normal renal function, and augmented renal clearance (ARC). We explored the use of therapeutic drug monitoring (TDM) to evaluate the concentration and efficacy of CAZ-AVI in CRKP-infected patients with different kidney statuses. Serum concentrations for CAZ and AVI were determined by the high-performance liquid chromatography method. Bacterial identification, routine susceptibility testing, renal function index, and others were performed in standard protocols in the hospital's clinical laboratories. In the two patients with ARC, in case 1, CAZ-AVI 2.5g q6h was used with good efficacy, and the concentrations were up to the pharmacokinetics/pharmacodynamics targets. In Case 2, 2.5 g q8h was used with invalid effectiveness, and AVI C was only 0.797 mg/l, which is lower than the PK/PD target. Case 3 was renal insufficiency using CAZ-AVI 1.25 q8h, and case 4 was normal renal function using 2.5 g q8h. Their concentrations were both up to the PK/PD targets. TDM results demonstrated that CAZ-AVI steady-state plasma concentration varies among patients with different kidney statuses, providing evidence for the utility of TDM of CAZ-AVI in individualized drug dose adjustment. ARC patients may need more CAZ-AVI daily doses than the standard dose.
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http://dx.doi.org/10.3389/fphar.2022.780991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257044PMC
June 2022

Copper-Lithium-Doped Nanohydroxyapatite Modulates Mesenchymal Stem Cells Homing to Treat Glucocorticoids-Related Osteonecrosis of the Femoral Head.

Front Bioeng Biotechnol 2022 2;10:916562. Epub 2022 Jun 2.

Orthopedic Research Institute, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, China.

tissue regeneration has been demonstrated to promote bone repair. To identify a better approach for treating osteonecrosis of the femoral head (ONFH), we prepared scaffolds using copper-lithium-doped nanohydroxyapatite (Cu-Li-nHA), which has the potential to modulate mesenchymal stem cells (MSCs) homing. The scaffold was fabricated using the gas foaming method and the migration, angiogenesis, and osteogenesis activities of MSCs were detected using Transwell assays, tube formation assays, alkaline phosphatase and alizarin red S staining, respectively. We then implanted the Cu-Li-nHA scaffold into the femoral heads of ONFH rabbits, and CFSE labeled exogenous MSCs were injected intravenously to verify cell homing. The repair effect was subsequently examined using micro-CT and histological analysis . The results showed that Cu-Li-nHA significantly promoted MSCs migration and homing by upregulating the HIF-1α/SDF-1 pathway. The Cu-Li-nHA group showed optimal osteogenesis and angiogenesis and greater improvements in new bone formation in ONFH rabbits. To summarize, Cu-Li-nHA promoted homing and induced the osteogenic differentiation of MSCs, thereby enhancing bone regeneration during ONFH repair. Thus, Cu-Li-nHA implantation may serve as a potential therapeutic strategy for ONFH in the future.
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http://dx.doi.org/10.3389/fbioe.2022.916562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201282PMC
June 2022

Identification of Upper Gastrointestinal Diseases during Screening Gastroscopy through Deep Convolutional Neural Network Algorithm.

Gastrointest Endosc 2022 Jun 16. Epub 2022 Jun 16.

Department of Clinical Research Center, Dazhou Central Hospital, Dazhou, Sichuan, China; Center of Intelligent Medicine, Computer Science, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Background And Aims: The clinical application of gastrointestinal endoscopy for the diagnosis of multiple diseases using artificial intelligence (AI) has been limited by its high false positive rates. There is an unmet need to develop a Gastrointestinal Endoscopy AI-assisted Diagnosis System (GEADS) to improve diagnostic accuracy and clinical utility.

Methods: In this retrospective, multicenter study, a convolutional neural network was trained to assess upper gastrointestinal diseases based on 26,228 endoscopic images from Dazhou Central Hospital that were randomly assigned (3:1:1) to the training dataset, validation dataset, and test dataset, respectively. To validate the model, 6 external independent datasets comprising 51,372 images of upper gastrointestinal diseases were collected. Besides, one prospective dataset comprising 27,975 images were collected. The performance of GEADS was compared with endoscopists with two professional degrees of expertise: expert, novice. 8 endoscopists in the expert group with more than 5 years of experience, 3 endoscopists in the novice group with 1 to 5 years of experience.

Results: The GEADS model achieved an accuracy of 0.918 (95% confidence interval [CI]: 0.914-0.922), with an F1 score of 0.884 (95% CI: 0.879-0.889), recall of 0.873 (95% CI: 0.868-0.878) and a precision of 0.890 (95% CI: 0.885-0.895) in the internal validation dataset. In the external validation datasets and one prospective validation dataset, the diagnostic accuracy of GEADS ranged from 0.841 (95% CI: 0.834-0.848) to 0.949 (95% CI: 0.935-0.963). With the help of GEADS, the diagnosing accuracies of novice and expert endoscopists were significantly improved (P < 0.001).

Conclusions: The AI system can assist endoscopists in improving the accuracy of diagnosing upper gastrointestinal diseases.
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http://dx.doi.org/10.1016/j.gie.2022.06.011DOI Listing
June 2022

Knockout Alleviates DSS-Induced Colitis Regulating the Immune Function of Dendritic Cells.

Front Immunol 2022 20;13:841254. Epub 2022 May 20.

Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Dysfunction of the immune system would disturb the intestinal homeostasis and lead to inflammatory bowel disease (IBD). Dendritic cells (DCs) help maintain intestinal homeostasis and immediately respond to pathogens or injuries once the mucosa barriers are destroyed during IBD. G protein-coupled receptors(GPR)174 is an essential regulator of immunity that is widely expressed in most immune cells, including DCs. However, the role of GPR174 in regulating the immune function of DC in colitis has not been investigated.

Methods: Dextran sodium sulfate (DSS) was administered to establish the mice colitis model. Data of weight, length of colon, disease activity index (DAI), and macroscopic scores were collected. The flow cytometry was used to detect the infiltrations of T cells and DCs, the mean fluorescence intensity (MFI) of CD80, CD86, CD40, and major histocompatibility complex-II (MHC-II). And T cells proliferataion was measured by carboxyfluorescein diacetate succinimidyl ester (CFSE). The expression of cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), interferon-γ (IFN-γ), interleukin -4 (IL-4)) and mRNA were measured by Elisa, quantitative polymerase chain reaction (qPCR), and immunofluorescence. RNA of bone-marrow-derived dendritic cells (BMDCs) was extracted for sequencing. Adoptive transfer of BMDCs was administrated intravenously.

Results: mice exposed to 3% DSS showed significant alleviation characterized by reduced loss of weight, more minor colon damage, and better DAI and macroscopic scores. The expression of pro-inflammatory cytokines (TNF-α, IL-6) decreased, while anti-inflammatory cytokine (IL-10) increased compared with WT mice. , BMDCs showed less maturity, with a declined expression of MHC-II, CD80, CD86 and reduced TNF-α, higher IL-10 after LPS stimulation. BMDCs were less capable of activating OT-II naïve CD4 T cells than WT BMDCs and induced more Th0 cells to differentiate into Treg while less into Th1. Furthermore, the transcriptome sequencing analysis exhibited that participated in TNF-α (NF-κB) signaling, leukocyte transendothelial migration, and Th1/Th2 cell differentiation pathways. Adoptive transfer of BMDCs to WT mice ameliorated DSS-induced colitis.

Conclusion: Our study indicated that was involved in the pathogenesis of IBD by regulating the maturation of the dendritic cells to maintain immune homeostasis. TNF-α (NF-κB) signaling pathway, leukocyte transendothelial migration, and Th1/Th2 cell differentiation pathways may be the target pathway.
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http://dx.doi.org/10.3389/fimmu.2022.841254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164256PMC
May 2022

The effects of polyethersulfone and Nylon 6 micromembrane filters on the pyraclostrobin detection: adsorption performance and mechanism.

Environ Sci Pollut Res Int 2022 May 28. Epub 2022 May 28.

College of Plant Protection, Hunan Agricultural University, Changsha, 410128, People's Republic of China.

Adsorption of test substances on micromembrane filters during sample pretreatment before qualitative and quantitative analysis has greatly affected the accuracy of the measurement. In the present study, it was found that the adsorption rate of pyraclostrobin reached 77.7-100% when water samples of pyraclostrobin (1 mL) were filtered with polyethersulfone (PES) and Nylon 6 filters. Therefore, the adsorption mechanisms were investigated from the kinetics, isotherms, and thermodynamics of the pyraclostrobin adsorption process, combined with attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) analysis. The results showed that PES accorded with second-order adsorption kinetics and Nylon 6 with first-order adsorption kinetics, and the correlation coefficient R was 0.98. The adsorption behavior of the two micromembranes followed the linear isothermal model, indicating that the adsorption process was through monolayer adsorption. Thermodynamic study showed that the adsorption of pyracoethyl on PES membrane was spontaneous endothermic, while that on Nylon 6 was spontaneous exothermic. The π-π electron-donor-acceptor (EDA) between pyraclostrobin and PES may promote the adsorption of PES to pyraclostrobin, and hydrogen bonding between pyraclostrobin and Nylon 6 micromembrane may be involved in the adsorption. Our study also proved that the adding 60% methanol and iodine solution (2 mmol/L) was an effective strategy to reduce the adsorption effects and to increase the accuracy of the detection.
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http://dx.doi.org/10.1007/s11356-022-21021-3DOI Listing
May 2022

The use of novel knotless barbed sutures in posterior long-segment lumbar surgery: a randomized controlled trial.

J Orthop Surg Res 2022 May 18;17(1):279. Epub 2022 May 18.

Department of Spinal Surgery, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Rd., Taijiang District, Fuzhou, 350005, Fujian Province, China.

Background: The study carries the aim to compare the clinical efficacy and economic outcomes of using barbed suture closure versus conventional closure for wounds after posterior long-segment lumbar surgery.

Methods: One hundred and eighty-one patients undertaking posterior long-segment lumbar surgery participated in the prospective randomized controlled trial study to receive either barbed suture wound closure (n = 91) or conventional suture closure (n = 90). Outcome measures included operating room time (ORT), wound closure time, length of incision, length of hospital stay (LOS), 90-day readmission rates, wound complications of dehiscence and infection, and costs.

Results: Barbed suture group was related with significantly lower ORT (P = 0.036), wound closure time (P < 0.001) and average wound closure time (P < 0.001), and significantly lower wound complication rates (dehiscence and infection) (P = 0.031). No significant differences were found when compared with conventional suture group in terms of length of incision (P = 0.086), length of hospital stay (P = 0.174), readmission rates up to 90 days after the surgical procedure (P = 0.232) and costs (P = 0.205).

Conclusion: The study suggested the knotless barbed suture technique outperformed the conventional suture in shortening operating room time, wound closure time and average wound closure time, and reducing wound complication rates.
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http://dx.doi.org/10.1186/s13018-022-03165-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118583PMC
May 2022

Highly selective extraction of uranium from wastewater using amine-bridged diacetamide-functionalized silica.

J Hazard Mater 2022 08 27;435:129022. Epub 2022 Apr 27.

Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, Sichuan 621900, PR China. Electronic address:

A major environmental concern related to nuclear energy is wastewater contaminated with uranium, thus necessitating the development of pollutant-reducing materials with efficiency and effectiveness. Herein, highly selective mesoporous silicas functionalized with amine-bridged diacetamide ligands SBA-15-ABDMA were prepared. Different spectroscopy techniques were used to probe the chemical environment and reactivity of the chelating ligands before and after sorption. The results showed that the functionalized SBA-15-ABDMA had a strong affinity for uranium at low pH (pH = 3) with desirable sorption capacity (68.82 mg/g) and good reusability (> 5). It showed excellent separation performance with a high distribution coefficient (K > 10 mL/g) and separation factors SF > 1000 at a pH of 3.5 in the presence of lanthanide nuclides, alkaline earth metal and transition metal ions. In particular, SiOspheres-ABDMA was used as a column material, which achieved excellent recovery of U(VI) (> 98%) and good reusability for samples of simulated mining and nuclear industries wastewater. XPS and crystallography studies clearly illustrated the tridentate coordination mode of U(VI)/PEABDMA and the mechanism and origin behind the high selectivity for U.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129022DOI Listing
August 2022

Simultaneously enhancing the photocatalytic and photothermal effect of NH-MIL-125-GO-Pt ternary heterojunction for rapid therapy of bacteria-infected wounds.

Bioact Mater 2022 Dec 29;18:421-432. Epub 2022 Mar 29.

School of Materials Science and Engineering, Peking University, Beijing, 100871, China.

Infections caused by bacteria threaten human health, so how to effectively kill bacteria is an urgent problem. We therefore synthesized a NH-MIL-125-GO-Pt ternary composite heterojunction with graphene oxide (GO) and platinum (Pt) nanoparticles co-doped with metal-organic framework (NH-MIL-125) for use in photocatalytic and photothermal synergistic disinfection under white light irradiation. Due to the good conductivity of GO and the Schottky junction between Pt and MOF, the doping of GO and Pt will effectively separate and transfer the photogenerated electron-hole pairs generated by NH-MIL-125, thereby effectively improving the photocatalytic efficiency of NH-MIL-125. Meanwhile, NH-MIL-125-GO-Pt has good photothermal effect under white light irradiation. Therefore, the NH-MIL-125-GO-Pt composite can be used for effective sterilization. The antibacterial efficiency of NH-MIL-125-GO-Pt against and were as high as 99.94% and 99.12%, respectively, within 20 min of white light irradiation. experiments showed that NH-MIL-125-GO-Pt could effectively kill bacteria and promote wound healing. This work brings new insights into the use of NH-MIL-125-based photocatalyst materials for rapid disinfection of environments with pathogenic microorganisms.
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http://dx.doi.org/10.1016/j.bioactmat.2022.03.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968451PMC
December 2022

2D Molybdenum Sulfide-Based Materials for Photo-Excited Antibacterial Application.

Adv Healthc Mater 2022 07 13;11(13):e2200360. Epub 2022 Apr 13.

School of Materials Science & Engineering, Peking University, Beijing, 100871, China.

Bacterial infections have seriously threatened human health and the abuse of natural or artificial antibiotics leads to bacterial resistance, so development of a new generation of antibacterial agents and treatment methods is urgent. 2D molybdenum sulfide (MoS ) has good biocompatibility, high specific surface area to facilitate surface modification and drug loading, adjustable energy bandgap, and high near-infrared photothermal conversion efficiency (PCE), so it is often used for antibacterial application through its photothermal or photodynamic effects. This review comprehensively summarizes and discusses the fabrication processes, structural characteristics, antibacterial performance, and the corresponding mechanisms of MoS -based materials as well as their representative antibacterial applications. In addition, the outlooks on the remaining challenges that should be addressed in the field of MoS are also proposed.
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http://dx.doi.org/10.1002/adhm.202200360DOI Listing
July 2022

Discovery of electric devil's staircase in perovskite antiferroelectric.

Sci Adv 2022 Apr 6;8(14):eabl9088. Epub 2022 Apr 6.

State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.

The devil's staircase, describing step-like function for two competing frequencies, is well known over a wide range of dynamic systems including Huyghens' clocks, Josephson junction, and chemical reaction. In condensed matter physics, the devil's staircase has been observed in spatially modulated structures, such as magnetic ordering. It draws widespread attentions because it plays a crucial role in the fascinating phenomena including phase-locking behaviors, commensurate-incommensurate phase transition, and spin-valve effect. Here, we report the observation of polymorphic phase transitions consisting of several steps in PbZrO-based system-namely, electric devil's staircase-originated from competing ferroelectric and antiferroelectric interactions. We fully characterize a specific electric dipole configuration by decomposing this competitive interaction in terms of basic structure and modulation function. Of particular interest is that the occurrence of many degenerate electric dipole configurations in devil's staircase enables superior energy storage performance. These observations are of great significance for exploring more substantive magnetic-electric correspondence and engineering practical high-power antiferroelectric capacitors.
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http://dx.doi.org/10.1126/sciadv.abl9088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986110PMC
April 2022

Ruxolitinib suppresses liver fibrosis progression and accelerates fibrosis reversal via selectively targeting Janus kinase 1/2.

J Transl Med 2022 04 5;20(1):157. Epub 2022 Apr 5.

Department of Hepatology, Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, No.13 Shiliugang Road, Guangzhou, 510315, Guangdong, China.

Background: JAK1 and JAK2 have been implicated in fibrosis and cancer as a fibroblast-related marker; however, their role in liver fibrosis has not been elucidated. Here, we aim to determine the effect and underlying mechanism of JAK1/2 inhibition on liver fibrosis and hepatic stellate cells (HSCs) and further explore the therapeutic efficacy of Ruxolitinib, a JAK1/2 selective inhibitor, on preventing and reversing liver fibrosis in mice.

Methods: Immunohistochemistry staining of JAK1 and JAK2 were performed on liver tissue in mice with hepatic fibrosis and human liver tissue microarray of liver cirrhosis and liver cancer. LX-2 cells treated with specific siRNA of JAK1 and JAK2 were used to analysis activation, proliferation and migration of HSCs regulated by JAK1/2. The effects of Ruxolitinib (JAK1/2 inhibitor) on liver fibrosis were studied in LX-2 cells and two progressive and reversible fibrosis animal models (carbon tetrachloride (CCl), Thioacetamide (TAA)).

Results: We found that JAK1/2 expression was positively correlated with the progression of HCC in humans and the levels of liver fibrosis in mice. Silencing of JAK1/2 down-regulated their downstream signaling and inhibited proliferation, migration, and activation of HSCs in vitro, while Ruxolitinib had similar effects on HSCs. Importantly, Ruxolitinib significantly attenuated fibrosis progression, improved cell damage, and accelerated fibrosis reversal in the liver of mice treated with CCl or TAA.

Conclusions: JAK1/2 regulates the function of HSCs and plays an essential role in liver fibrosis and HCC development. Its inhibitor, Ruxolitinib, may be an effective drug for preventing and treating liver fibrosis.
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http://dx.doi.org/10.1186/s12967-022-03366-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981941PMC
April 2022

Polymyxin B-Based Regimens for Patients Infected with Carbapenem-Resistant Gram-Negative Bacteria: Clinical and Microbiological Efficacy, Mortality, and Safety.

Infect Drug Resist 2022 22;15:1205-1218. Epub 2022 Mar 22.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, 410011, People's Republic of China.

Background: The increasing prevalence of carbapenem-resistant Gram-negative bacteria (CR-GNB) represents a global healthcare crisis. This study explored the efficacy and safety of Polymyxin B (PMB)-based regimens and factors influencing their effectiveness.

Methods: Patients with CR-GNB infections treated with PMB for more than three days were enrolled in this retrospective study from 1st June 2018 to 30th April 2020. Data were collected on patient characteristics, bacterial culture, and drug-sensitivity test results; anti-infection treatment regimens, particularly details of PMB use; and adverse drug reactions. Clinical and microbiological efficacy, mortality, and safety of PMB-based regimens in CR-GNB infected patients were evaluated. Univariate analysis and multivariate logistic regression analyses were used to assess factors influencing efficacy and mortality.

Results: A total of 373 CR-GNB strains were cultured from 268 patients. About 41.04% of patients used PMB loading dose of 1.01 (0.84-1.69) mg/kg. Maintenance dose was 0.85 (0.82-1.00) mg/kg q12h. The clinical efficacy rate was 36.57% (98/268), the total bacterial clearance rate of PMB was 39.42%, and the all-cause mortality rate was 33.96%. The adverse drug reaction rate was 19.58%, among which the incidence of renal toxicity was highest (8.21%). Multivariate logistic regression analysis showed that clinical efficacy, bacterial clearance rate, and all-cause mortality were associated with patient-related facts, including mechanical ventilation use, underlying diseases (such as respiratory disease), the type and site of CR-GNB infection, and PMB administration timing and loading dose.

Conclusion: PMB is a relatively safe and effective antibiotic drug for treatment of critically ill patients with CR-GNB infection; however, PMB use should be subject to guidelines recommendations for early administration, loading administration, and adequate administration, which could help to improve the clinical efficacy, microbiological efficacy, and mortality.
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http://dx.doi.org/10.2147/IDR.S357746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957303PMC
March 2022

Efficacy and safety of a bridging strategy that uses intravenous platelet glycoprotein receptor inhibitors for patients undergoing surgery after coronary stent implantation: a meta-analysis.

BMC Cardiovasc Disord 2022 03 24;22(1):125. Epub 2022 Mar 24.

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Road, Yuanjiagang, Yuzhong District, Chongqing, 400016, China.

Background: Current guidelines indicate we can consider a bridging strategy that uses intravenous, reversible glycoprotein inhibitors for patients that required surgery following recent stent implantation. However, no strong clinical evidence exists that demonstrates the efficacy and safety of this treatment. Therefore, in this study, the efficacy and safety of a bridging strategy that uses intravenous platelet glycoprotein receptor inhibitors will be evaluated.

Methods: A meta-analysis was performed on preoperative bridging studies in patients undergoing coronary stent surgery. The primary outcome was the success rate of no major adverse cardiovascular events (MACE). The secondary outcomes were the success rate of no reoperations to stop bleeding.

Results: A total of 10 studies that included 382 patients were used in this meta-analysis. For the primary endpoint, the success rate was 97.7% (95% CI 94.4-98.0%) for glycoprotein IIb/IIIa inhibitors, 98.8% (95% CI 96.0-100%) for tirofiban (6 studies) and 95.8% (95% CI 90.4-99.4%) for eptifibatide (4 studies). For secondary endpoints, the success rate was 98.0% (95% CI 94.8-99.9%) for glycoprotein IIb/IIIa inhibitors, 99.7% (95% CI 97.1-100%) for tirofiban (5 studies), and 95.3% (95% CI 88.5-99.4%) for eptifibatide (4 studies).

Conclusion: The results of this study showed that the use of intravenous platelet glycoprotein IIb/IIIa inhibitors as a bridging strategy might be safe and effective for patients undergoing coronary stent implantation that require surgery soon after.
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http://dx.doi.org/10.1186/s12872-022-02563-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953042PMC
March 2022

Multifunctional Baicalin-Modified Contact Lens for Preventing Infection, Regulating the Ocular Surface Microenvironment and Promoting Corneal Repair.

Front Bioeng Biotechnol 2022 2;10:855022. Epub 2022 Mar 2.

Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, The Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China.

Corneal injury inevitably leads to disruption of the ocular surface microenvironment, which is closely associated with delayed epithelial cell repair and the development of infection. Recently, drug-loaded therapeutic contact lenses have emerged as a new approach to treating corneal injury due to their advantages of relieving pain, promoting corneal repair, and preventing infection. However, few therapeutic contact lenses could modulate the ocular surface's inflammation and oxidative stress microenvironment. To address this, in this study, we covalently immobilized multifunctional baicalin (BCL), a flavon molecular with anti-inflammatory, anti-oxidative stress, and antibacterial capabilities, onto the surface of the contact lens. The BCL-modified contact lens showed excellent optical properties, powerful antibacterial properties, and non-toxicity to endothelial cells. Furthermore, the BCL-modified contact lens could significantly modulate the ocular surface microenvironment, including inhibition of macrophage aggregation and resistance to epithelium damage caused by oxidative stress. In animal models, BCL-modified corneal contact lens effectively promoted corneal epithelial cells repair. These excellent properties suggested that multifunctional BCL molecules had great application potential in the surface engineering of ophthalmic medical materials.
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http://dx.doi.org/10.3389/fbioe.2022.855022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926214PMC
March 2022

Recent Progress of Chronic Stress in the Development of Atherosclerosis.

Oxid Med Cell Longev 2022 8;2022:4121173. Epub 2022 Mar 8.

Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, No. 1 DaHua Road, Dong Dan, Beijing 100730, China.

With the development of the times, cardiovascular diseases have become the biggest cause of death in the global aging society, causing a serious social burden. Atherosclerosis is a chronic inflammatory disease, which can occur in large and medium-sized blood vessels in the whole body. It takes atherosclerotic plaque as the typical pathological change and endothelial injury as the core pathophysiological mechanism. It is the pathological basis of coronary heart disease, peripheral artery disease, cerebrovascular disease, and other diseases. Recent studies have shown that chronic stress plays an important role in the occurrence and development of atherosclerosis, endothelial injury, lipid metabolism, and chronic inflammation. This process involves a large number of molecular targets. It is usually the cause of atherosclerotic cardiovascular and cerebrovascular diseases. If chronic stress factors exist for a long time, patients have genetic susceptibility, and the combination of environmental factors triggers the pathogenesis, which may eventually lead to complete blockage of the blood vessels, unstable rupture of plaques, and serious adverse cardiovascular events. This paper reviews the role of chronic stress in the occurrence and development of atherosclerosis, focusing on the pathophysiological mechanism.
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http://dx.doi.org/10.1155/2022/4121173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923806PMC
March 2022

The enhanced photocatalytic sterilization of MOF-Based nanohybrid for rapid and portable therapy of bacteria-infected open wounds.

Bioact Mater 2022 Jul 2;13:200-211. Epub 2021 Nov 2.

Biomedical Materials Engineering Research Center, Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan 430062, China.

Open wounds are prone to infection and difficult to heal, which even threatens the life of patients because bacterial infections can induce other lethal complications without prompt treatment. The commonly used antibiotics treatment for bacterial infections has been reported to cause globally bacterial resistance and even the occurrence of superbacteria. The highly effective and antibiotic-independent therapeutic strategies are urgently needed for treating various kinds of bacteria-infected diseases. In this work, we synthesized an eco-friendly nanohybrid material (ZnDMZ) consisting of a kind of biodegradable metal organic framework (MOF, ZIF-8) combined with Zn-doped MoS (Zn-MoS) nanosheets, which exhibited great ability to kill bacteria and promote the healing of bacteria-infected wounds under 660 nm light irradiation. The underlying mechanism is that besides the local hyperthermia, the nanohybrid material exhibits enhanced photocatalytic performance than single component in it, , it can also be excited by 660 nm light to produce more oxygen radical species (ROS) due to the following factors. On one hand, the Zn doping can reduce the work function and the band gap of MoS, which promotes the movement of photoexcited electrons to the surface of the material. On the other hand, the combination between Zn-MoS and MOF induces the formation of a built-in electric field due to their work function difference, thus accelerating the separation of photoexcited electron-hole pairs. Because of the synergy of photocatalytic effect, photothermal effect and the released Zn ions, the synthesized ZnDMZ possessed a highly effective antibacterial efficacy of 99.9% against under 660 nm light irradiation for 20 min without cytotoxicity. tests showed that this nanohybrid material promoted the wound healing due to the released Zn ions. This nanohybrid will be promising for rapid and portable treatment of bacteria-infected open wounds in pathogenic bacteria contaminated environments.
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http://dx.doi.org/10.1016/j.bioactmat.2021.10.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843951PMC
July 2022

Dysfunction of FadA-cAMP signalling decreases Aspergillus flavus resistance to antimicrobial natural preservative Perillaldehyde and AFB1 biosynthesis.

Environ Microbiol 2022 03 22;24(3):1590-1607. Epub 2022 Feb 22.

School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.

Aspergillus flavus is an opportunistic fungal pathogen that colonizes agriculture crops with aflatoxin contamination. We found that Perillaldehyde (PAE) effectively inhibited A. flavus viability and aflatoxin production by inducing excess reactive oxygen species (ROS). Transcriptome analysis indicated that the Gα protein FadA was significantly induced by PAE. Functional characterization of FadA showed it is important for asexual development and aflatoxin biosynthesis by regulation of cAMP-PKA signalling. The ΔfadA mutant was more sensitive to PAE, while ΔpdeL and ΔpdeH mutants can tolerate excess PAE compared to wild-type A. flavus. Further RNA-sequence analysis showed that fadA was important for expression of genes involved in oxidation-reduction and cellular metabolism. The flow cytometry and fluorescence microscopy demonstrated that ΔfadA accumulated more concentration of ROS in cells, and the transcriptome data indicated that genes involved in ROS scavenging were downregulated in ΔfadA mutant. We further found that FadA participated in regulating response to extracellular environmental stresses by increasing phosphorylation levels of MAPK Kinase Slt2 and Hog1. Overall, our results indicated that FadA signalling engages in mycotoxin production and A. flavus resistance to antimicrobial PAE, which provide valuable information for controlling this fungus and AF biosynthesis in pre- and postharvest of agricultural crops.
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http://dx.doi.org/10.1111/1462-2920.15940DOI Listing
March 2022

Acetylcholine deficiency disrupts extratelencephalic projection neurons in the prefrontal cortex in a mouse model of Alzheimer's disease.

Nat Commun 2022 02 22;13(1):998. Epub 2022 Feb 22.

Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou, 570228, China.

Short-term memory deficits have been associated with prefrontal cortex (PFC) dysfunction in Alzheimer's disease (AD) and AD mouse models. Extratelencephalic projection (ET) neurons in the PFC play a key role in short-term working memory, but the mechanism between ET neuronal dysfunction in the PFC and short-term memory impairment in AD is not well understood. Here, using fiber photometry and optogenetics, we found reduced neural activity in the ET neurons in the medial prefrontal cortex (mPFC) of the 5×FAD mouse model led to object recognition memory (ORM) deficits. Activation of ET neurons in the mPFC of 5×FAD mice rescued ORM impairment, and inhibition of ET neurons in the mPFC of wild type mice impaired ORM expression. ET neurons in the mPFC that project to supramammillary nucleus were necessary for ORM expression. Viral tracing and in vivo recording revealed that mPFC ET neurons received fewer cholinergic inputs from the basal forebrain in 5×FAD mice. Furthermore, activation of cholinergic fibers in the mPFC rescued ORM deficits in 5×FAD mice, while acetylcholine deficiency reduced the response of ET neurons in the mPFC to familiar objects. Taken together, our results revealed a neural mechanism behind ORM impairment in 5×FAD mice.
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http://dx.doi.org/10.1038/s41467-022-28493-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863829PMC
February 2022

Sunset Without AIDS: protocol for a randomized controlled trial of a brief video-based intervention to improve the ability of AIDS prevention in elderly men.

Trials 2022 Feb 14;23(1):146. Epub 2022 Feb 14.

Department of Nursing, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Background: Although progress has been made in the prevention and treatment of HIV in China, there are still a considerable number of new infections annually, especially in older men. HIV health education for older men is urgently needed. Evidence-based, acceptable, and scalable interventions are urgently needed to increase their capacity to prevent HIV. We describe a trial protocol to evaluate the effectiveness of a brief video-based intervention targeting older men's knowledge of HIV prevention.

Design: This study is a randomized controlled trial. The trial will be held in the residents' activity centers of three communities.

Methods: A total of 450 older men will be randomly divided into three groups (Sunset Without AIDS intervention group and two control groups) for 2 weeks. We will assess the feasibility and acceptability of intervention through interviews. The primary outcome includes changes in participants' knowledge related to AIDS after 2 weeks intervention and 1, 3, and 6 months of follow-up. The secondary outcomes, e.g., changes in participants' stigma attitude, risk behaviors attitude, and risk behaviors related to AIDS, will be respectively assessed 2 weeks, 1 month, 3 months, and 6 months after the second intervention.

Discussion: Sunset Without AIDS may be an innovative way to help older men improve HIV prevention knowledge, fill the gap in video-based HIV prevention education for the older men in China, and gain experience of HIV education. This project will innovate the HIV education ideas of older men and enrich the theoretical research content of AIDS-related education of older men. The findings may also provide the basis for the research and formulation of more reasonable AIDS education strategies, prevention, and control policies for the elderly.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100045708 . Registered on 23 April 2021.
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http://dx.doi.org/10.1186/s13063-022-06069-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843026PMC
February 2022

Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production.

Molecules 2022 Jan 18;27(3). Epub 2022 Jan 18.

State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Jinan 250100, China.

Several strategies, including inducer addition and biosensor use, have been developed for dynamical regulation. However, the toxicity, cost, and inflexibility of existing strategies have created a demand for superior technology. In this study, we designed an optogenetic dual-switch system and applied it to increase polyhydroxybutyrate (PHB) production. First, an optimized chromatic acclimation sensor/regulator (RBS10-CcaS#10-CcaR) system (comprising an optimized ribosomal binding site (RBS), light sensory protein CcaS, and response regulator CcaR) was selected for a wide sensing range of approximately 10-fold between green-light activation and red-light repression. The RBS10-CcaS#10-CcaR system was combined with a blue light-activated YF1-FixJ-PhlF system (containing histidine kinase YF1, response regulator FixJ, and repressor PhlF) engineered with reduced crosstalk. Finally, the optogenetic dual-switch system was used to rewire the metabolic flux for PHB production by regulating the sequences and intervals of the citrate synthase gene () and PHB synthesis gene () expression. Consequently, the strain RBS34, which has high expression and a time lag of 3 h, achieved the highest PHB content of 16.6 wt%, which was approximately 3-fold that of F34 (expressed at 0 h). The results indicate that the optogenetic dual-switch system was verified as a practical and convenient tool for increasing PHB production.
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http://dx.doi.org/10.3390/molecules27030617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838604PMC
January 2022

Celastrol Attenuates Psoriasiform Inflammation by Targeting the IRF1/GSTM3 Axis.

J Invest Dermatol 2022 Aug 5;142(8):2281-2285.e11. Epub 2022 Feb 5.

Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai, China; Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.jid.2022.01.018DOI Listing
August 2022

Nephrotoxicity and Efficacy Assessment of Polymyxin B Use in Renal Transplant Patients.

Infect Drug Resist 2022 26;15:275-283. Epub 2022 Jan 26.

Department of Pharmacy, The Second Xiangya Hospital, Central South University; Institute of Clinical Pharmacy, Central South University, Changsha, 410011, People's Republic of China.

Purpose: This study investigates the nephrotoxicity and efficacy assessment of polymyxin B (PMB) use in renal transplant patients.

Patients And Methods: This retrospective study included adult (>18 years of age) renal transplant patients who received PMB intravenous drip for more than 72 hours. Efficacy assessment of PMB included clinical treatment efficacy, microbiological efficacy at the end of PMB treatment, and in-hospital all-cause mortality. Nephrotoxicity of PMB was evaluated for further group comparison.

Results: We enrolled 235 renal transplant patients in our study. After PMB treatment, 45 patients occurred PMB-nephrotoxicity, and the nephrotoxicity rate was 19.15%. Among them, 44 patients were RIFLE R stage, and one patient was RIFLE I stage. The dose of PMB used in patients was 40.0 (40.0-50.0) mg q12h with a loading dose of 41.8±9.8 mg. Multivariate logistic regression analysis showed that ICU admission, vasoactive agents, aminoglycosides, creatinine clearance rate before PMB use, and mean total hospital stay were independent risk factors of PMB-nephrotoxicity in kidney transplant patients. The clinical effective rate was 97.9%, and the microbiological clean rate was 66.7%.

Conclusion: Our study demonstrated that PMB low dose regimens might achieve good efficacy and less nephrotoxicity in renal transplant patients. We should evaluate the severity of the infection and renal function of patients, avoid the combined use of other nephrotoxic drugs, and minimize the course of use to reduce the occurrence of PMB-nephrotoxicity.
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http://dx.doi.org/10.2147/IDR.S348571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801393PMC
January 2022

Red Yeast Rice for Hyperlipidemia: A Meta-Analysis of 15 High-Quality Randomized Controlled Trials.

Front Pharmacol 2021 17;12:819482. Epub 2022 Jan 17.

Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.

RYR is a commonly used lipid-lowering dietary supplements in Asian and European countries, showing considerable benefits and low toxicity. This quantitative study aims to present high-quality evidence regarding the efficacy and safety of RYR in treating hyperlipidemia, in order to promote its clinical application. PubMed, embase, and Cochrane Central Register of Controlled Trials databases were systematically searched, and high-quality randomized controlled trials comparing RYR with non-RYR interventions were included. RevMan5.3 software was used to conduct the meta-analysis. A total of 1,012 individuals participated in this study (481 in the experimental and 531 in the control group). In comparison to statins, RYR was more effective in lowering TG (MD, -19.90; 95% CI, -32.22 to -7.58; = 0.002), comparable in lowering LDL-C and elevating HDL-C, and less effective in lowering TC (MD, 12.24; 95% CI, 2.19 to 22.29; = 0.02). Compared with nutraceutical, RYR significantly reduced TC (MD, -17.80; 95% CI, -27.12 to -8.48; = 0.0002) and LDL-C (MD, -14.40; 95% CI, -22.71 to -6.09; = 0.0007), and elevated HDL-C (MD, 7.60; 95% CI, 4.33 to 10.87; < 0.00001). Moreover, RYR effectively synergized nutraceutical to further reduce TC (MD, -31.10; 95% CI, -38.83 to -23.36; < 0.00001), LDL-C (MD, -27.91; 95% CI, -36.58 to -19.24; < 0.00001), and TG (MD, -26.32; 95% CI, -34.05 to -18.59; < 0.00001). Additionally, RYR significantly reduced apoB (MD, -27.98; 95% CI, -35.51 to -20.45; < 0.00001) and, whether alone or in combination, did not increase the risk of adverse events in patients with hyperlipidemia. RYR at 200-4800 mg daily appears to be a safe and effective treatment for hyperlipidemia, effectively regulating blood lipid levels with an exceptional impact on TG. Looking forward, high-quality clinical trials with longer observation periods are required to evaluate the efficacy and safety of RYR as a long-term medication. : (https://www.crd.york.ac.uk/PROSPERO/), identifier (CRD4202128450).
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http://dx.doi.org/10.3389/fphar.2021.819482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802088PMC
January 2022

Tobacco smoking was positively associated with metabolic syndrome among patients with psoriasis in Shanghai: A cross-sectional study.

Tob Induc Dis 2022 22;20:05. Epub 2022 Jan 22.

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Introduction: A number of studies have reported a high correlation between psoriasis and metabolic syndrome (MetS), and tobacco smoking is one independent risk factor accounting for the increased prevalence both for psoriasis and MetS. However, few studies have been conducted to assess the effects of tobacco smoking on co-morbidities of psoriasis and MetS.

Methods: We conducted a cross-sectional study with 1014 psoriasis patients recruited from January to May 2021. Patients were recruited with a cluster survey method in Yueyang Hospital (affiliated with Shanghai University of Traditional Chinese Medicine) and Shanghai Skin Diseases Hospital (affiliated with Tongji University). Data were collected by face-to-face questionnaire interviews which included basic information, personal life habits, medical history, and clinical examinations. SPSS 24.0 was used for data analysis and a p<0.05 was considered statistically significant.

Results: The 1014 psoriasis patients were predominantly males (65.58%), with an average age of 45.98 years (IQR: 34.00-57.00). Of these, 25.74% (261) of psoriasis had MetS and 31.85% (323) were tobacco smokers. Male psoriasis patients had higher tobacco smoking prevalence than female patients. With increasing age and BMI, the prevalence of tobacco smoking among psoriasis patients increased dramatically (p<0.01). Logistic regression indicated that psoriasis patients with tobacco smoking had 1.78 times (95% CI: 1.21-2.60) the probability to have MetS than those without tobacco smoking, even adjusting for potential confounding factors. Moreover, smoking psoriasis patients with MetS consumed more cigarettes per day, with longer smoking duration, but with an older age of smoking initiation.

Conclusions: The prevalence of tobacco smoking and MetS among psoriasis patients was high in Shanghai, and tobacco smoking was positively associated with the MetS among psoriasis patients. Clinicians should recommend psoriasis patients to abstain from tobacco smoking and provide tobacco cessation assistance regularly.
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http://dx.doi.org/10.18332/tid/144228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780213PMC
January 2022
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