Publications by authors named "Yue Liu"

1,919 Publications

  • Page 1 of 1

Breaking the nanoparticle's dispersible limit via rotatable surface ligands.

Nat Commun 2022 Jun 23;13(1):3581. Epub 2022 Jun 23.

Institute for Composites Science Innovation, School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Achieving versatile dispersion of nanoparticles in a broad range of solvents (e.g., water, oil, and biofluids) without repeatedly recourse to chemical modifications are desirable in optoelectronic devices, self-assembly, sensing, and biomedical fields. However, such a target is limited by the strategies used to decorate nanoparticle's surface properties, leading to a narrow range of solvents for existing nanoparticles. Here we report a concept to break the nanoparticle's dispersible limit via electrochemically anchoring surface ligands capable of sensing the surrounding liquid medium and rotating to adapt to it, immediately forming stable dispersions in a wide range of solvents (polar and nonpolar, biofluids, etc.). Moreover, the smart nanoparticles can be continuously electrodeposited in the electrolyte, overcoming the electrode surface-confined low throughput limitation of conventional electrodeposition methods. The anomalous dispersive property of the smart Ag nanoparticles enables them to resist bacteria secreted species-induced aggregation and the structural similarity of the surface ligands to that of the bacterial membrane assists them to enter the bacteria, leading to high antibacterial activity. The simple but massive fabrication process and the enhanced dispersion properties offer great application opportunities to the smart nanoparticles in diverse fields.
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http://dx.doi.org/10.1038/s41467-022-31275-7DOI Listing
June 2022

Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Allohexaploids.

Plants (Basel) 2022 Jun 13;11(12). Epub 2022 Jun 13.

Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T & R Base of Zhengzhou University, Zhengzhou 450002, China.

Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in allohexaploids and provides more resourceful genetic information on the reproductive biology of polyploid breeding.
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http://dx.doi.org/10.3390/plants11121556DOI Listing
June 2022

Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.

Stem Cell Res Ther 2022 Jun 17;13(1):253. Epub 2022 Jun 17.

Nankai University School of Medicine, 94 Weijin Road, Tianjin, 300071, China.

Background: Intravenous administration of mesenchymal stromal cells (MSCs) has an acknowledged competence of cardiac repair, despite a lack of systematic description of the underlying biological mechanisms. The lung, but not the heart, is the main trapped site for intravenously transplanted MSCs, which leaves a spatial gap between intravenously transplanted MSCs and the injured myocardium. How lung-trapped MSCs after intravenous transplantation rejuvenate the injured myocardium remains unknown.

Methods: MSCs were isolated from human placenta tissue, and DF-MSCs or Gluc-MSCs were generated by transduced with firefly luciferase (Fluc)/enhanced green fluorescence protein (eGFP) or Gaussia luciferase (Gluc) lactadherin fusion protein. The therapeutic efficiency of intravenously transplanted MSCs was investigated in a murine model of doxorubicin (Dox)-induced cardiotoxicity. Trans-organ communication from the lung to the heart with the delivery of blood was investigated by testing the release of MSC-derived extracellular vesicles (MSC-EVs), and the potential miRNA inner MSC-EVs were screened out and verified. The potential therapeutic miRNA inner MSC-EVs were then upregulated or downregulated to assess the further therapeutic efficiency RESULTS: Dox-induced cardiotoxicity, characterized by cardiac atrophy, left ventricular dysfunction, and injured myocardium, was alleviated by consecutive doses of MSCs. These cardioprotective effects might be attributed to suppressing GRP78 triggering endoplasmic reticulum (ER) stress-induced apoptosis in cardiomyocytes. Our results confirmed that miR-181a-5p from MSCs-derived EVs (MSC-EVs) inhibited GRP78. Intravenous DF-MSCs were trapped in lung vasculature, secreted a certain number of EVs into serum, which could be confirmed by the detection of eGFP EVs. GLuc activity was increased in serum EVs from mice administrated with GLuc-MSCs. MiR-181a-5p, inhibiting GRP78 with high efficacy, was highly expressed in serum EVs and myocardium after injecting consecutive doses of MSCs into mice treated with Dox. Finally, upregulation or downregulation of miR-181a-5p levels in MSC-EVs enhanced or weakened therapeutic effects on Dox-induced cardiotoxicity through modulating ER stress-induced apoptosis.

Conclusions: This study identifies intravenously transplanted MSCs, as an endocrine reservoir, to secrete cardioprotective EVs into blood continuously and gradually to confer the trans-organ communication that relieves Dox-induced cardiotoxicity.
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http://dx.doi.org/10.1186/s13287-022-02922-zDOI Listing
June 2022

Integrated Microbiome and Host Transcriptome Profiles Link Parkinson's Disease to Genus: Evidence From Feces, Blood, and Brain.

Front Microbiol 2022 26;13:875101. Epub 2022 May 26.

Department of Geriatric Neurology, Shaanxi Provincial People's Hospital, Xi'an, China.

A link between the gut microbiome and Parkinson's disease (PD) has been intensively studied, and more than 100 differential genera were identified across the studies. However, the predominant genera contributing to PD remain poorly understood. Inspired by recent advances showing microbiota distribution in the blood and brain, we, here, comprehensively investigated currently available fecal microbiome data (1,914 samples) to identify significantly altered genera, which were further validated by comparison to the results from microbiome analysis of blood (85 samples) and brain (268 samples). Our data showed that the composition of fecal microbiota was different from that of blood and brain. We found that was the unique genus consistently depleted across feces, blood, and brain samples of PD patients ( < 0.05), despite using rigorous criteria to remove contaminants. Moreover, enrichment analyses revealed that host genes correlated with genus abundance were mainly involved in mitochondrial function and energy metabolism, and mapped to neurodegenerative diseases (NDDs) and metabolic diseases. A random forest classifier constructed with fecal microbiota data demonstrated that genus was an important feature contributing to discriminating PD patients from controls [receiver operating characteristic (ROC)-area under curve (AUC) = 0.704, precision-recall curve (PRC)-AUC = 0.787]. Through the integration of microbiome and transcriptome, our study depicted microbial profiles in the feces, blood, and brain of PD patients, and identified genus as a potential genus linked to PD. Further studies are greatly encouraged to determine the role of genus in the pathogenesis of PD.
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http://dx.doi.org/10.3389/fmicb.2022.875101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204254PMC
May 2022

Perspective and Experience of Male Nursing Students in 3-year Vocational College During Their Clinical Practicum: A Qualitative Study in Shanghai, China.

Front Public Health 2022 30;10:905200. Epub 2022 May 30.

Nursing Department, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China.

Background: With the expansion of nursing enrollment, more and more male students are joining the nursing program. Nevertheless, the number of male nurses who actually stay in clinical work in China continues to be low. Clinical practicum is a critical period during which nursing students transform to the role of registered nurses. However, few studies have researched the perspective and experience during clinical practicum among male nursing students in 3-year vocational colleges in China.

Objectives: To explore the perspective and experience of Chinese male nursing students during clinical practicum in the third year. One of the main objectives is to provide implications for future nursing education and to help male nursing students for better adaptation to clinical roles.

Methods: The qualitative study used a purposive sampling method and collected data through in-depth, semi-structured interviews. The participants were 20 male students from a 3-year vocational college in Shanghai. They completed the basic nursing course in college and underwent a clinical practicum in the 3rd level hospitals in China between July 2020 and March 2021. A Seven-stage Colaizzi process was used for data analysis.

Results: Three themes were revealed through content analysis: opportunities and challenges brought by gender factors in hospital humanistic environment, gaining experience and growth during clinical practicum, and future career planning.

Conclusions: The presented findings further our understanding of the perspective and experience of male nursing students during clinical practicum. It is very important and necessary for providing implications for future nursing education. At the same time, effective support from society, such as social unions, mass media, government, can help better address male nursing students' needs and complete the role transform of registered nurses.
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http://dx.doi.org/10.3389/fpubh.2022.905200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197777PMC
June 2022

[Panax notoginseng: a review on chemical components, chromatographic analysis, P. notoginseng extracts, and pharmacology in recent five years].

Zhongguo Zhong Yao Za Zhi 2022 May;47(10):2584-2596

National Institutes for Food and Drug Control Beijing 102629, China.

As a famous and precious Chinese medicinal material, Panax notoginseng(PN) has been commonly used for a long history in China. As reported, PN exhibits significant pharmacological actions in protecting cardiocerebral vascular system and nervous system and suppressing tumors. In recent years, with the innovation in ideas, as well as the development of methods and equipment, PN has been extensively investigated, and notable progress has been made. This paper reviewed the advancements of PN in recent five years from chemical components, chromatographic analysis, P. notoginseng extracts, and pharmacology, in which the application of PN extracts in quality control was first summarized. The present study aims to provide a theoretical basis for quality control, product development, and rational medication of PN.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211220.202DOI Listing
May 2022

Deep residual-SVD network for brain image registration.

Phys Med Biol 2022 Jun 17. Epub 2022 Jun 17.

School of Cyber Science and Engineering, Zhengzhou University North Campus, No. 97, Wenhua Road, Wenhua Road Street, Jinshui District, Zhengzhou, 450002, CHINA.

Objective: Medical image registration aims to find the deformation field that can align two images in a spatial position. A medical image registration method based on U-Net architecture has been proposed currently. However, U-Net architecture has few training parameters, which leads to weak learning ability, and it ignores the adverse effects of image noise on the registration accuracy. The article aims at addressing the problem of weak network learning ability and the adverse effects of noisy images on registration.

Approach: Here we propose a novel unsupervised 3D brain image registration framework, which introduces the residual unit and singular value decomposition (SVD) denoising layer on the U-Net architecture. Residual unit solves the problem of network degradation, that is, registration accuracy becomes saturated and then degrades rapidly with the increase in network depth. SVD denoising layer uses the estimated model order for SVD-based low-rank image reconstruction. we use Akaike information criterion to estimate the appropriate model order, which is used to remove noise components. We use the exponential linear unit (ELU) as the activation function, which is more robust to noise than other peers.

Main Results: The proposed method is evaluated on the publicly available brain MRI datasets: Mindboggle101 and LPBA40. Experimental results demonstrate our method outperforms several state-of-the-art methods for the metric of Dice Score. The mean number of folding voxels and registration time are comparable to state-of-the-art methods.

Significance: This study shows that Deep Residual-SVD Network can improve registration accuracy. This study also demonstrate that the residual unit can enhance the learning ability of the network, the SVD denoising layer can denoise effectively, and the ELU is more robust to noise.
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http://dx.doi.org/10.1088/1361-6560/ac79faDOI Listing
June 2022

SDHA/B reduction promotes hepatocellular carcinoma by facilitating the deNEDDylation of cullin1 and stabilizing YAP/TAZ.

Hepatology 2022 Jun 17. Epub 2022 Jun 17.

Institute of Pharmacology & Toxicology, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

Background & Aims: Succinate dehydrogenase enzyme (SDH) is frequently diminished in Hepatocellular carcinoma (HCC) patient samples, and SDH reduction is associated with elevated succinate level and poor prognosis in HCC patients. But the underlying mechanisms about how impaired SDH activity promotes HCC remain unclear.

Approach & Results: In this study, we observed remarkable downregulations of SDH subunits A and B (SDHA/B) in chronic liver injury-induced murine HCC models and patient samples. Subsequent RNA sequencing, hematoxylin & eosin (H&E) staining and immunohistochemistry (IHC) analyses of HCC samples revealed that Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) were significantly upregulated in HCC, with their levels inversely correlating with that of SDHA/B. YAP/TAZ stability was greatly enhanced in SDHA/B-depleted HCC cells along with accumulation of succinate. Further mechanistic analyses demonstrated that impaired activity of SDHA/B resulted in succinate accumulation which facilitated the deNEDDylation of cullin1, therefore disrupted the E3 ubiquitin ligase SCF complex, consequently led to YAP/TAZ stabilization and activation in HCC cells. The accelerated in vitro cell proliferation and in vivo tumor growth caused by SDHA/B reduction or succinate exposure were largely dependent on the aberrant activation of YAP/TAZ.

Conclusions: Our study demonstrated that SDHA/B reduction promotes HCC proliferation by preventing the proteasomal degradation of YAP/TAZ through modulating cullin1 NEDDylation, thus addicts SDH-deficient HCC cells to YAP/TAZ pathway and renders these cells vulnerable to YAP/TAZ inhibition. Our findings warrant further investigation on the therapeutic effects of targeting YAP/TAZ in HCC patients displaying reduced SDHA/B or elevated succinate levels.
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http://dx.doi.org/10.1002/hep.32621DOI Listing
June 2022

[Comparative study of microscope assisted minimally invasive anterior fusion and mobile microendoscopic discectomy assisted fusion for lumbar degenerative diseases].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Jun;36(6):672-680

Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Tianjin, 300211, P. R. China.

Objective: To investigate the effectiveness of microscope assisted anterior lumbar discectomy and fusion (ALDF) and mobile microendoscopic discectomy assisted lumbar interbody fusion (MMED-LIF) for lumbar degenerative diseases.

Methods: A clinical data of 163 patients with lumbar degenerative diseases who met the criteria between January 2018 and December 2020 was retrospectively analyzed. Fifty-three cases were treated with microscope assisted ALDF (ALDF group) and 110 cases with MMED-LIF (MMED-LIF group). There was no significant difference between the two groups in terms of gender, age, disease type, surgical segments, preoperative visual analogue scale (VAS) scores of low back pain and leg pain, Oswestry disability index (ODI), intervertebral space height, lordosis angle, and spondylolisthesis rate of the patients with lumbar spondylolisthesis ( >0.05). The operation time, intraoperative blood loss, and hospital stay of the two groups were recorded. The effectiveness was evaluated by VAS scores of low back pain and leg pain and ODI. Postoperative lumbar X-ray films were taken to observe the position of Cage and measure the intervertebral space height, lordosis angle, and spondylolisthesis rate of the patients with lumbar spondylolisthesis.

Results: The operations were successfully completed in both groups. The operation time, intraoperative blood loss, and hospital stay in ALDF group were less than those in MMED-LIF group ( <0.05). The patients in both groups were followed up 12-36 months, with an average of 24 months. The VAS scores of low back pain and leg pain and ODI after operation were lower than those before operation in the two groups, and showed a continuous downward trend, with significant differences between different time points ( <0.05). There were significant differences between two groups in VAS score of low back pain and ODI ( <0.05) and no significant difference in VAS score of leg pain ( >0.05) at each time point. The improvement rates of VAS score of low back pain and ODI in ALDF group were significantly higher than those in MMED-LIF group ( =7.187, =0.000; =2.716, =0.007), but there was no significant difference in the improvement rate of VAS score of leg pain ( =0.556, =0.579). The postoperative lumbar X-ray films showed the significant recovery of the intervertebral space height, lordosis angle, and spondylolisthesis rate at 2 days after operation when compared with preoperation ( <0.05), and the improvements were maintained until last follow-up ( >0.05). The improvement rates of intervertebral space height and lordosis angle in ALDF group were significantly higher than those in MMED-LIF group ( <0.05). There was no significant difference in the reduction rate of spondylolisthesis between the two groups ( =1.396, =0.167). During follow-up, there was no loosening or breakage of the implant and no displacement or sinking of the Cage.

Conclusion: Under appropriate indications, microscope assisted ALDF and MMED-LIF both can achieve good results for lumbar degenerative diseases. Microscope assisted ALDF was superior to MMED-LIF in the improvement of low back pain and function and the recovery of intervertebral space height and lordosis angle.
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http://dx.doi.org/10.7507/1002-1892.202202039DOI Listing
June 2022

Integrated bioinformatics analysis reveals marker genes and immune infiltration for pulmonary arterial hypertension.

Sci Rep 2022 Jun 16;12(1):10154. Epub 2022 Jun 16.

Department of Cardiovascular, The Third Affiliated Hospital of Chongqing Medical University, No. 1 Shuanghu Branch Road, Chongqing, 401120, China.

Pulmonary arterial hypertension (PAH) is a chronic cardiopulmonary syndrome with high pulmonary vascular load and eventually causing RV heart failure even death. However, the mechanism of pulmonary hypertension remains unclear. The purpose of this research is to detect the underlying key genes and potential mechanism of PAH using several bioinformatic methods. The microarrays GSE22356, GSE131793 and GSE168905 were acquired from the GEO. Subsequently, a host of bioinformatics techniques such as DAVID, STRING, R language and Cytoscape were utilized to investigate DEGs between PAH and healthy controls and conduct GO annotation, KEGG enrichment analysis and PPI network construction etc. Additionally, we predicted the transcription factors regulating DEGs through iRegulon plugin of Cytoscape and CIBERSORT was used to conduct immune infiltration analysis. One thousand two hundred and seventy-seven DEGs (403 up-regulated and 874 down-regulated) were identified from peripheral blood samples of 32 PAH patients and 29 controls, among which SLC4A1, AHSP, ALAS2, CA1, HBD, SNCA, HBM, SELENBP1, SERPINE1 and ITGA2B were detected as hub genes. The functional enrichment changes of DEGs were mainly enriched in protein binding, extracellular exosome, extracellular space, extracellular region and integral component of plasma membrane. The hub genes are chiefly enriched at extracellular exosome, hemoglobin complex, blood microparticle, oxygen transporter activity. Among TF-DEGs network, 42 target DEGs and 6 TFs were predicted with an NES > 4 (TEAD4, TGIF2LY, GATA5, GATA1, GATA2, FOS). Immune infiltration analysis showed that monocytes occupied the largest proportion of immune cells. The trend analysis results of infiltration immune cells illustrated that PAH patients had higher infiltration of NK cell activation, monocyte, T cell CD4 memory activation, and mast cell than healthy controls and lower infiltration of T cell CD4 naive. We detected SLC4A1, AHSP, ALAS2, CA1, HBD, SNCA, HBM, SELENBP1, SERPINE1 and ITGA2B as the most significant markers of PAH. The PAH patients had higher infiltration of NK cell activation, monocyte, T cell CD4 memory activation, and mast cell than healthy controls and lower infiltration of T cell CD4 naive. These identified genes and these immune cells probably have precise regulatory relationships in the development of PAH.
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http://dx.doi.org/10.1038/s41598-022-14307-6DOI Listing
June 2022

Metabolomic profiling of cerebrospinal fluid reveals an early diagnostic model for central nervous system involvement in acute lymphoblastic leukaemia.

Br J Haematol 2022 Jun 16. Epub 2022 Jun 16.

School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai, China.

The pathogenesis of central nervous system involvement (CNSI) in patients with acute lymphoblastic leukaemia (ALL) remains unclear and a robust biomarker of early diagnosis is missing. An untargeted cerebrospinal fluid (CSF) metabolomics analysis was performed to identify independent risk biomarkers that could diagnose CNSI at the early stage. Thirty-three significantly altered metabolites between ALL patients with and without CNSI were identified, and a CNSI evaluation score (CES) was constructed to predict the risk of CNSI based on three independent risk factors (8-hydroxyguanosine, l-phenylalanine and hypoxanthine). This predictive model could diagnose CNSI with positive prediction values of 95.9% and 85.6% in the training and validation sets respectively. Moreover, CES score increased with the elevated level of central nervous system (CNSI) involvement. In addition, we validated this model by tracking the changes in CES at different stages of CNSI, including before CNSI and during CNSI, and in remission after CNSI. The CES showed good ability to predict the progress of CNSI. Finally, we constructed a nomogram to predict the risk of CNSI in clinical practice, which performed well compared with observed probability. This unique CSF metabolomics study may help us understand the pathogenesis of CNSI, diagnose CNSI at the early stage, and sequentially achieve personalized precision treatment.
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http://dx.doi.org/10.1111/bjh.18307DOI Listing
June 2022

Sequence and functional features of a novel scavenger receptor homolog, SCARA5 from Yellow drum (Nibea albiflora).

Dev Comp Immunol 2022 Jun 8;135:104463. Epub 2022 Jun 8.

National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, PR China. Electronic address:

As an important member in SR-As, member 5 (SCARA5) can swallow apoptotic cells and foreign bodies, and participate multiple signaling pathways to inhibit tumor occurrence, development growth and metastasis. To explore its immune function, SCARA5 was identified from the yellow drum (Nibea albiflora) according to its transcriptome data, and its full-length cDNA was 6968 bp (named as NaSCARA5, GenBank accession no: MW070211) encoding 497 amino acids with a calculated molecular weight of 55.12 kDa, which had the typical motifs of SR family, such as transmembrane helix region, coil region, Pfam collagens region and SR region. BLASTp and the phylogenetic relationship analysis illustrated that the sequences shared high similarity with known SCARA5 of teleosts. Quantitative real time RT-PCR analysis showed that NaSCARA5 was expressed in intestine, stomach, liver, kidney, gill, heart and spleen, with the highest in the spleen (24.42-fold compared with that in heart). After being infected with Polyinosinic:polycytidylic acid (PolyI:C), Vibrio alginolyticus and Vibrio parahaemolyticus, NaSCARA5 mRNA were up-regulated with time dependent mode in spleen, which suggested that NaSCARA5 might play an important role in the immune process of fish. The extracellular domain of NaSCARA5 was successfully expressed in BL21 (DE3), and yielded the target protein of the expected size with many active sites for their conferring protein-protein interaction functions. After being purified by Ni-NAT Superflow resin and renatured, it was found to bind all the tested bacteria (V.parahaemolyticus,V.alginolyticus and Vibrio harveyi). The eukaryotic expression vector of the NaSCARA5-EGFP fusion protein was constructed and transferred into epithelioma papulosum cyprini (EPC) cells, and it was mainly expressed on the cell membrane indicating that NaSCARA5 was a typical transmembrane protein. The aforementioned results indicated that NaSCARA5 played a significant role in the defense against pathogenic bacteria infection as PRRs, which may provide some further understandings of the regulatory mechanisms in the fish innate immune system for SR family.
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http://dx.doi.org/10.1016/j.dci.2022.104463DOI Listing
June 2022

Long-term exposure to bisphenol A and its analogues alters the behavior of marine medaka (Oryzias melastigma) and causes hepatic injury.

Sci Total Environ 2022 Jun 8;841:156590. Epub 2022 Jun 8.

Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address:

Bisphenols (BPA, BPF, and BPAF) are widely present in the aquatic environment and have various adverse effects on aquatic organisms. However, their hepatic toxicity in marine fish is not fully understood. Hence, we examined the growth parameters, histological features, antioxidant defense mechanisms, and gene expression profiles in the livers of marine medaka after exposure to single and combined bisphenols for 70 days. The final body weight and final body length of males exposed to BPAF were significantly higher than those in the control group, and the survival rate was significantly lower. Bisphenol exposure led to vacuolization and local lesions in the liver, especially in the BPAF group, and altered antioxidant enzyme activity in the liver, leading to oxidative stress. In addition, after bisphenol exposure, marine medaka showed anxiolytic effects and a significant reduction in swimming distance compared with that in the control group. As determined by RNA-seq, bisphenol exposure altered multiple biological pathways in the liver, such as fatty acid biosynthesis, fatty acid metabolism, and ribosome biogenesis pathways, with significant changes in gene expression levels. In particular, chgs and vtgs were significantly up-regulated after BPAF exposure, suggesting an estrogenic effect. Overall, bisphenols can adversely affect the growth and metabolism of marine medaka. BPF and BPAF may not be ideal substitutes for BPA.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156590DOI Listing
June 2022

Quantifying the contributions of climate change and human activities to vegetation dynamic in China based on multiple indices.

Sci Total Environ 2022 Sep 9;838(Pt 4):156553. Epub 2022 Jun 9.

College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.

Distinguishing the respective roles of climate change and anthropogenic activities can provide crucial information for sustainable management of the environment. Here, using the residual trend method (RESTREND), which measures the residue of the actual and potential trends of vegetation, we quantified the relative contributions of human activities (e.g., ecological restoration, overgrazing, and urbanization) and climate change (the warmer and wetter trend) to vegetation dynamics in China during 1988-2018 based on multiple vegetation indices, including the vegetation optical depth (Ku-VOD, C-VOD), normalized difference vegetation index (NDVI), and gross primary productivity (GPP). The results showed that the VOD, NDVI, and GPP exhibited overall increasing trends during 1988-2018. Human activities contributed >70% to the increases in NDVI and GPP in China, whereas a counterbalanced contribution of human activities and climate change was identified for the VOD dynamics (51% vs. 49%). Regions with high contributions from human activities to NDVI, GPP, and VOD were located in northeastern, southern, central, and northwestern China. In northern China, the positive impacts of human activities on NDVI (78%) and BEPS-GPP (83%) were greater than those of climate change. In contrast, human activities contributed 96% to the decrease in Ku-VOD over the same period. Before 2000, climate change promoted increases in GPP and NDVI in most regions of southern China. The increasing rates of GPP and NDVI accelerated after 2000 due to afforestation. However, human activities like overgrazing and urbanization have led to decreases in Ku-VOD in northern and southwestern China, and in C-VOD in northeastern, eastern, central, southwestern, and southern China. In all, the relative roles of climate and human factors varied in different regions when NDVI, GPP, or VOD were individually considered. Our results highlighted that the regional-scale vegetation conditions should be taken into full account to achieve sustainable management of ecosystems.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156553DOI Listing
September 2022

Development of a self-help cognitive behavioral therapy programme for reducing the stigma of stroke survivors: a modified delphi study.

Top Stroke Rehabil 2022 Jun 10:1-15. Epub 2022 Jun 10.

Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Background: Stigma is a common psychological consequence for stroke survivors that aggravates their physical and psychological burden and hinders their rehabilitation. Currently, there are few interventions targeted at the stigma of stroke survivors.

Objectives: This study reports on the development of a self-help cognitive behavioral therapy (CBT) programme driven by a logical model of stigma in stroke survivors, CBT and the advice of multidomain experts.

Methods: A logical model of stigma in stroke survivors was derived from a systematic search of the literature and semistructured interviews with 21 patients to identify factors influencing stigma. The item content of the programme was generated based on this logical model in combination with CBT. A modified Delphi process with an expert panel of multidomain experts was used to evaluate and refine the content of the programme. SPSS 20.0 was used for data analysis.

Results: Seventeen experts accepted the invitation to participate, and all completed two rounds of the Delphi survey. Six sections and 26 items were identified. Consensus was reached among experts that the self-help CBT programme included the following six sections: health education, understanding stigma, cognition change, skills training and self-care, self-acceptance and relapse prevention.

Conclusions: The self-help CBT programme includes health education and psychological education. This study extends the limited body of research on stroke-related stigma interventions, and the next step is to evaluate its efficacy in trials.
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http://dx.doi.org/10.1080/10749357.2022.2083296DOI Listing
June 2022

A Visualized Dynamic Prediction Model for Overall Survival in Elderly Patients With Pancreatic Cancer for Smart Medical Services.

Front Public Health 2022 24;10:885624. Epub 2022 May 24.

Scientific Research Department, First People's Hospital of Zigong City, Zigong, China.

Background: Pancreatic cancer (PC) is a highly malignant tumor of the digestive system. The number of elderly patients with PC is increasing, and older age is related to a worse prognosis. Accurate prognostication is crucial in treatment decisions made for people diagnosed with PC. However, an accurate predictive model for the prognosis of these patients is still lacking. We aimed to construct nomograms for predicting the overall survival (OS) of elderly patients with PC.

Methods: Patients with PC, older than 65 years old from 2010 to 2015 in the Surveillance, Epidemiology, and End Results database, were selected and randomly divided into training cohort ( = 4,586) and validation cohort ( = 1,966). Data of patients in 2016-2018 ( = 1,761) were used for external validation. Univariable and forward stepwise multivariable Cox analysis was used to determine the independent prognostic factors. We used significant variables in the training set to construct nomograms predicting prognosis. The performance of the models was evaluated for their discrimination and calibration power based on the concordance index (C-index), calibration curve, and the decision curve analysis (DCA).

Results: Age, insurance, grade, surgery, radiation, chemotherapy, T, N, and American Joint Commission on Cancer were independent predictors for OS and thus were included in our nomogram. In the training cohort and validation cohort, the C-indices of our nomogram were 0.725 (95%CI: 0.715-0.735) and 0.711 (95%CI: 0.695-0.727), respectively. The 1-, 3-, and 5-year areas under receiver operating characteristic curves showed similar results. The calibration curves showed a high consensus between observations and predictions. In the external validation cohort, C-index (0.797, 95%CI: 0.778-0.816) and calibration curves also revealed high consistency between observations and predictions. The nomogram-related DCA curves showed better clinical utility compared to tumor-node-metastasis staging. In addition, we have developed an online prediction tool for OS.

Conclusions: A web-based prediction model for OS in elderly patients with PC was constructed and validated, which may be useful for prognostic assessment, treatment strategy selection, and follow-up management of these patients.
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http://dx.doi.org/10.3389/fpubh.2022.885624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171143PMC
June 2022

Mechanically Contacted Distributed-Feedback Optical Microcavity.

Nanomaterials (Basel) 2022 May 31;12(11). Epub 2022 May 31.

Institute of Information Photonics Technology, Beijing University of Technology, Beijing 100124, China.

We report a construction of distributed-feedback (DFB) optical microcavities, which is realized through mechanical contact between a high-quality planar thin film of a polymeric semiconductor and a large-area homogeneous nanograting. Using poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-(benzo[2,1,3] thiadiazol-4,8-diyl)] (F8BT) as the active medium for the planar layer, we achieve strong amplified spontaneous emission from such a microcavity with a low threshold. This not only simplifies largely the fabrication techniques for DFB microcavities, but also avoids the unexpected chemical interactions during solution processing between the organic semiconductors and the nanograting materials. Furthermore, high-quality polymer thin films with high surface smoothness and high thickness homogeneity are employed without any modulations for constructing the microcavities. This also suggests new designs of microcavity light-emitting diodes, or even for realizing electrically pumped polymer lasers, simply by metallizing the dielectric nanogratings as the electrodes.
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http://dx.doi.org/10.3390/nano12111883DOI Listing
May 2022

Recent Advances for the Developing of Instant Flavor Peanut Powder: Generation and Challenges.

Foods 2022 May 24;11(11). Epub 2022 May 24.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, China.

Instant flavor peanut powder is a nutritional additive that can be added to foods to impart nutritional value and functional properties. Sensory acceptability is the premise of its development. Flavor is the most critical factor in sensory evaluation. The heat treatment involved in peanut processing is the main way to produce flavor substances and involves chemical reactions: Maillard reaction, caramelization reaction, and lipid oxidation reaction. Peanut is rich in protein, fat, amino acids, fatty acids, and unsaturated fatty acids, which participate in these reactions as volatile precursors. N-heterocyclic compounds, such as the pyrazine, are considered to be the key odorants of the "baking aroma". However, heat treatment also affects the functional properties of peanut protein (especially solubility) and changes the nutritional value of the final product. In contrast, functional properties affect the behavior of proteins during processing and storage. Peanut protein modification is the current research hotspot in the field of deep processing of plant protein, which is an effective method to solve the protein denaturation caused by heat treatment. The review briefly describes the characterization and mechanism of peanut flavor during heat treatment combined with solubilization modification technology, proposing the possibility of using peanut meal as material to produce IFPP.
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http://dx.doi.org/10.3390/foods11111544DOI Listing
May 2022

Effect of radiofrequency catheter ablation on left atrial structure and function in patients with different types of atrial fibrillation.

Sci Rep 2022 Jun 9;12(1):9511. Epub 2022 Jun 9.

Division of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Radiofrequency catheter ablation (RFCA) is widely used to treat atrial fibrillation (AF), but its effect on left atrial (LA) remodeling in patients with AF is not completely clarified. Few studies have reported the changes in structure and function of the left atrium in patients with different types of AF after RFCA. To analyze the effect of RFCA on the LA structure and function in patients with nonvalvular paroxysmal AF, persistent AF and long-standing persistent AF (LSPAF). RFCA was performed in 180 patients with paroxysmal AF, persistent AF and LSPAF. The changes of LA structure and function in echocardiogram and speckle-tracking echocardiography findings were compared before the procedure, and at 1, 2, 3, 4 weeks, and 2, 3, 6, and 9-12 months after the procedure. There were 60 patients in the paroxysmal AF group, 60 in the persistent AF group and 60 patients in LSPAF group. The pre-procedure LA diameter and volume were smaller in the paroxysmal AF group than persistent AF and LSPAF group. There was no significant change of in the LA structure and function in the paroxysmal AF group within 1 year. In the persistent AF and LSPAF groups, LA structure (anteroposterior diameter, LA volume) significantly decreased, but remained larger than that in paroxysmal AF group. In persistent and LSPAF, function (LA ejection fraction, strain, strain rate) increased significantly within 1 week, then gradually increased. RFCA improved the LA structure and function and resulted in heart reverse remodeling, especially for persistent AF and LSPAF.
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http://dx.doi.org/10.1038/s41598-022-13725-wDOI Listing
June 2022

Identification of KRBA1 as a Potential Prognostic Biomarker Associated with Immune Infiltration and m6A Modification in Hepatocellular Carcinoma.

J Hepatocell Carcinoma 2022 31;9:497-516. Epub 2022 May 31.

Department of Thyroid Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, 330000, People's Republic of China.

Purpose: Hepatocellular carcinoma (HCC) is a malignancy with high incidence, but its prognosis is not optimistic. KRBA1 is a member of the KRAB family and participates in the regulation of gene transcription. However, no studies have focused on the role of KRBA1 in HCC.

Patients And Methods: In this study, we first analyzed the expression of KRBA1 in HCC using TCGA and ICGC databases and validated by Immunohistochemistry in clinical HCC samples. The Wilcoxon rank-sum test was used to determine the relationship between KRBA1 expression and clinicopathological features. Subsequently, we used Kaplan-Meier online website analysis and Cox regression model to predict the prognostic value of KRBA1 in HCC patients. Furthermore, the functions of KRBA1 were identified by enrichment analysis. TIMER and GSCALite were used to investigate the relationship between KRBA1 expression in HCC and immune infiltration and drug targets, respectively. Finally, the relationship between KRBA1 expression and m6A modification in HCC was analyzed using the TCGA and ICGA datasets.

Results: The results showed that KRBA1 was upregulated in HCC and was associated with many clinicopathological features. High KRBA1 causes poor overall survival and may be an independent risk factor for HCC. KRBA1 tends to be hypermethylated and associated with poor prognosis in HCC compared with normal tissues. Enrichment analysis indicates that KRBA1 is associated with cell cycle and immune processes, and TIMER analysis shows that KRBA1 expression is associated with infiltration levels and immune characteristics of various immune cells. Silenced KRBA1 evidently reduced three chemokine expression in HCC cells. Drug sensitivity analysis showed that KRBA1 was sensitive to 39 drug small molecules. KRBA1 showed a strong positive correlation with five m6A related genes.

Conclusion: KRBA1 is a prognostic biomarker associated with HCC immunity and m6a modification, serving as an effective target for the diagnosis and treatment of HCC.
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http://dx.doi.org/10.2147/JHC.S363862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166909PMC
May 2022

Artificial Intelligence-Based CT Imaging on Diagnosis of Patients with Lumbar Disc Herniation by Scalpel Treatment.

Comput Intell Neurosci 2022 27;2022:3688630. Epub 2022 May 27.

Department of Medical Administration, Shandong Provincial Third Hospital, Jinan 250031, Shandong, China.

The aim of this study was to explore the application effect of computed tomography (CT) image based on active contour segmentation algorithm in the treatment of lumbar disc herniation (LDH) with scalpel. 78 patients with LDH were selected and divided into a lateral crypt block treatment group (group A) and a scalpel treatment group (group B) randomly. All the patients were examined by lumbar CT images based on artificial intelligence (AI) algorithm. Then, the clinical efficacy and Japanese orthopedic association (JOA) and visual analogue scale (VAS) scores were compared between the two groups. It was found that the total effective rate in group B was higher (92.31% vs. 84.62%) (  0.05). After treatment, the disc height (DH) in group A was obviously lower, and the vertebral body slippage was obviously higher (  0.05) than before. After treatment, there were more patients with nerve root location changes, edema, or disappearance in group B (  0.05). In contrast with JOA and VAS scores before treatment, both the groups showed obvious differences after treatment, especially group B (  0.05). Therefore, the CT images based on the AI algorithm can be used to analyze the treatment effect of LDH, and the scalpel treatment was more effective.
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http://dx.doi.org/10.1155/2022/3688630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167027PMC
June 2022

Underlying mechanisms of promoted formation of haloacetic acids disinfection byproducts after indometacin degradation by non-thermal discharge plasma.

Water Res 2022 Jul 31;220:118701. Epub 2022 May 31.

College of Natural Resources and Environment, Northwest A and F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China.

Indometacin (IDM), as a kind of non-steroidal anti-inflammatory drugs, has ecological and health risks, which is the potential precursor of chlorination disinfection byproducts (DBPs). Non-thermal discharge plasma was attempted to eliminate IDM and control subsequent DBPs formation. Satisfactory removal performance for IDM was realized by the plasma oxidation; almost 100% of IDM was removed within 2 min. Relatively greater removal efficiency was gained at a higher plasma voltage and a lower pH level. Electron paramagnetic resonance spectrometer revealed that reactive species ·OH, O·, and O were responsible for IDM decomposition. Based on analyses of Fourier transform infrared spectroscopy, two-dimensional correlation spectroscopy, three-dimensional fluorescence spectrum, and gas chromatography-mass spectrometer, attacks of reactive species resulted in sequence breakages in functional groups of IDM, leading to production of small molecular alcohols, acids, and amines. Possible decomposition pathways of IDM were proposed. The produced acetamide and 1H-indol-5-ol were important precursors of DBPs. Formation and toxicity of nitrogen-containing DBPs were dramatically inhibited after IDM degradation; however, those of haloacetic acids were strengthened. The relevant roadmaps among DBPs and degradation intermediates were figured out. This study revealed the underlying mechanisms of IDM degradation by discharge plasma and its potential risks in chlorination disinfection.
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http://dx.doi.org/10.1016/j.watres.2022.118701DOI Listing
July 2022

Strategies to Improve the Thermoelectric Figure of Merit in Thermoelectric Functional Materials.

Front Chem 2022 19;10:865281. Epub 2022 May 19.

College of Material Science Engineer, Liaoning Technical University, Fuxin, China.

In recent years, thermoelectric functional materials have been widely concerned in temperature difference power generation, electric refrigeration and integrated circui, and so on. In this paper, the design and research progress of thermoelectric materials around lifting value in recent years are reviewed. Optimizing the carrier concentration to improve the Seebeck coefficient, the steady improvement of carrier mobility and the influence of energy band engineering on thermoelectric performance are discussed. In addition, the impact of lattice thermal conductivity on value is also significant. We discuss the general law that the synergistic effect of different dimensions, scales, and crystal structures can reduce lattice thermal conductivity, and introduce the new application of electro-acoustic decoupling in thermoelectric materials. Finally, the research of thermoelectric materials is summarized and prospected in the hope of providing practical ideas for expanding the application and scale industrialization of thermoelectric devices.
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http://dx.doi.org/10.3389/fchem.2022.865281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160435PMC
May 2022

miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus.

J Exp Bot 2022 Jun 5. Epub 2022 Jun 5.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that the transcription factors (TFs) CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes CsSPLs and reduction of SE efficiency. In the STTM-miR156a overexpressed callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated; csi-miR172d was down-regulated, whereas the target genes CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus to alter starch accumulation level in callus cells and regulate SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs mediated regulation of SE, and provides clues to enhancing SE capability in citrus.
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http://dx.doi.org/10.1093/jxb/erac248DOI Listing
June 2022

WheatCENet: A database for comparative co-expression networks analysis of allohexaploid wheat and its progenitors.

Genomics Proteomics Bioinformatics 2022 Jun 1. Epub 2022 Jun 1.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

Genetic and epigenetic changes after polyploidization events could result in variable gene expression and modified regulatory networks. Here, using large-scale transcriptome data, we constructed co-expression networks for diploid, tetraploid, and hexaploid wheat species, and built a platform for comparing co-expression networks of allohexaploid wheat and its progenitors, named WheatCENet. WheatCENet is a platform for searching and comparing specific functional co-expression networks, as well as identifying the related functions of the genes clustered therein. Functional annotations like pathway, gene family, protein-protein interactions, microRNA (miRNA), and several lines of epigenome data are integrated into this platform, and gene ontology (GO) annotation, gene set enrichment analysis (GSEA), motif identification, and other useful tools are also included. Using WheatCENet, we found that the network of WHEAT ABERRANT PANICLE ORGANIZATION 1 (WAPO1) has more co-expressed genes related to spike development in hexaploid wheat than its progenitors. We also found a novel motif of CCWWWWWWGG (CArG) specifically in the promoter region of WAPO-A1, suggesting that neofunctionalization of the WAPO-A1 gene affects spikelet development in hexaploid wheat. WheatCENet is useful for investigating co-expression networks and conducting other analyses, and thus facilitates comparative and functional genomic studies in wheat. WheatCENet is freely available at http://bioinformatics.cpolar.cn/ WheatCENet and http://bioinformatics.cau.edu.cn/WheatCENet.
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http://dx.doi.org/10.1016/j.gpb.2022.04.007DOI Listing
June 2022

Gut microbiota and atopic dermatitis in children: a scoping review.

BMC Pediatr 2022 Jun 2;22(1):323. Epub 2022 Jun 2.

Department of Acupuncture and Tuina Science, School of Traditional Chinese Medicine, Naval Medical University, No. 800 Xiangyin Road, Shanghai, 200433, China.

Background: Gut microbiota plays an important role in the development of atopic dermatitis (AD). We aimed to elucidate research trends in gut microbiota and AD in children, to provide evidence and insights to the clinical prevention and treatment of AD in children.

Methods: A scoping literature review on the studies of gut microbiota and AD were conducted. Two authors independently searched Pubmed et al. databases for studies focused on gut microbiota and AD in children up to January 15, 2022. The literatures were screened and analyzed by two reviewers.

Results: A total of 44 reports were finally included and analyzed. Current researches have indicated that abnormal human microecology is closely associated with AD, and the disturbance of intestinal microbiota plays an important role in the occurrence and development of AD. Probiotics can correct the microbiota disorder, have the functions of regulating immunity, antioxidant, and help to restore the microecological homeostasis. However, there is still a lack of high-quality research reports on the efficacy and safety of probiotics in the prevention and treatment of AD in children.

Conclusions: The changes of gut microbiota are essential to the development of AD in children, which may be an effective target for the prevention and treatment of AD. Future studies with larger sample size and rigorous design are needed to elucidate the effects and safety of probiotics in AD.
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http://dx.doi.org/10.1186/s12887-022-03390-3DOI Listing
June 2022

Fe-Based Theranostic Agents Respond to the Tumor Microenvironment for MRI-Guided Ferroptosis-/Apoptosis-Inducing Anticancer Therapy.

ACS Biomater Sci Eng 2022 Jun 2;8(6):2610-2623. Epub 2022 Jun 2.

Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, P. R. China.

Tumor microenvironment-specific magnetic resonance imaging (MRI) contrast agents are conducive to accurate diagnoses by visualization of biochemical and pathological changes for suitable treatment. Herein, we reported a pH-responsive contrast agent DFeZd NP with MRI diagnosis and tumor treatment capabilities. DFeZd NPs can map the pH change by modulating the MR signal in different acid-base environments. Moreover, T1 signals are stronger in the tumor site, which proves efficient in distinguishing malignant tumors from normal tissues, as well as demarcating the tumor boundary. Subsequently, sustained supply of Fe through the Fe-based contrast agent leads to Fe redox cycling and lipid peroxides, inducing ferroptosis in tumor cells. Furthermore, under an acidic tumor microenvironment, in the presence of ascorbic acid, increased Fe is generated, which serves as a stronger inducer of ferroptosis. Moreover, due to the different relaxivity of Fe and Fe, redox cycling and ferroptosis in tumors can be monitored by MRI. Therefore, we propose DFeZd NPs as accessible and promising Fe-based dopamine-derived contrast agents for specific MRI imaging and ferroptosis induction for anticancer therapy.
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http://dx.doi.org/10.1021/acsbiomaterials.1c01626DOI Listing
June 2022

SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.

J Virol 2022 Jun 2;96(12):e0041222. Epub 2022 Jun 2.

Beijing Institute of Biotechnology, Beijing, China.

SARS-CoV-2 is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19) and poses a significant threat to global health. N protein (NP), which is a major pathogenic protein among betacoronaviruses, binds to the viral RNA genome to allow viral genome packaging and viral particle release. Recent studies showed that NP antagonizes interferon (IFN) induction and mediates phase separation. Using live SARS-CoV-2 viruses, this study provides solid evidence showing that SARS-CoV-2 NP associates with G3BP1 and G3BP2 and . NP could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming G3BP1-mediated antiviral innate immunity. G3BP1 conditional knockout mice (, -Cre) exhibit significantly higher lung viral loads after SARS-CoV-2 infection than wild-type mice. Our findings contribute to the growing body of knowledge regarding the pathogenicity of NP and provide insight into new therapeutics targeting NP. In this study, by assay and live SARS-CoV-2 virus infection, we provide solid evidence that the SARS-CoV-2 NP associates with G3BP1 and G3BP2 and . NP could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming antiviral innate immunity mediated by G3BP1 in A549 cell lines and G3BP1 conditional knockout mice (-cKO) mice, which provide in-depth evidence showing the mechanism underlying NP-related SARS-CoV-2 pathogenesis through G3BPs.
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http://dx.doi.org/10.1128/jvi.00412-22DOI Listing
June 2022

Effect of Clemastine Fumarate on Perioperative Hemodynamic Instability Mediated by Anaphylaxis During Cardiopulmonary Bypass Surgery.

Med Sci Monit 2022 Jun 2;28:e936367. Epub 2022 Jun 2.

Department of Anesthesiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (mainland).

BACKGROUND Perioperative hemodynamic instability mediated by anaphylaxis is a life-threatening complication in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). This study aimed to evaluate the effect of clemastine fumarate in this specific patient population. MATERIAL AND METHODS We enrolled 100 participants who met the inclusion criteria and randomly allocated them to the treatment group and the placebo group. Participants in the treatment group and the placebo group were treated separately with an injection of clemastine fumarate and saline, respectively. Plasma histamine concentration and blood pressure were quantified at 5 timepoints during the perioperative period, and differences between the 2 groups were assessed by repeated-measures ANOVA. The postoperative complications and in-hospital mortality also were evaluated. All participants were followed up for 7 days after cardiac surgery. RESULTS Plasma histamine concentrations increased in both groups but were statistically significantly lower in the treatment group during the perioperative period (P=0.007). Diastolic blood pressure (P=0.014) and mean arterial pressure (P=0.024) in the treatment group were significantly higher than in the placebo group during the perioperative period. The coefficients of variation for systolic (13.9±4.2% vs 17.2±4.4%, P<0.01) and diastolic (12.9±4.9% vs 15.3±5.2%, P=0.02) blood pressure were significantly lower in the treatment group compared with the placebo group. CONCLUSIONS Pretreatment with clemastine fumarate restrains the increase in histamine concentration and provides safer hemodynamics in patients undergoing cardiac surgery with CPB.
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http://dx.doi.org/10.12659/MSM.936367DOI Listing
June 2022

Replication protein RPA2A regulates floral transition by cooperating with PRC2 in Arabidopsis.

New Phytol 2022 May 28. Epub 2022 May 28.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

RPA2A is a subunit of the conserved heterotrimeric replication protein A (RPA) in Arabidopsis, which is an essential replisome component that binds to single-stranded DNA during DNA replication. RPA2A controls a set of developmental processes, but the underlying mechanism is largely unknown. Here we show that RPA2A represses key flowering genes including FLOWERING LOCUS T (FT), AGAMOUS (AG), and AGAMOUS LIKE 71 (AGL71) to suppress floral transition by cooperating with the PRC2 complex. RPA2A is vigorously expressed in dividing cells and required for proper DNA replication. Mutation of RPA2A leads to early flowering, which is dependent on ectopic expression of key flowering genes including FT molecularly and genetically. RPA2A and PRC2 have common target genes including FT, AG, and AGL71 supported by genetic analysis, transcriptome profiling, and H3K27me3 ChIP-seq analysis. Furthermore, RPA2A physically interacts with PRC2 components CLF, EMF2, and MSI1, which recruits CLF to the chromatin loci of FT, AG, and AGL71. Together, our results show that the replication protein RPA2A recruits PRC2 to key flowering genes through physical protein interaction, thereby repressing the expression of these genes to suppress floral transition in Arabidopsis.
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http://dx.doi.org/10.1111/nph.18279DOI Listing
May 2022
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