Publications by authors named "Yue Liu"

1,515 Publications

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High-performance microwave absorption of MOF-derived [email protected] carbon anchored on carbon foam.

J Colloid Interface Sci 2021 Jun 3;602:197-206. Epub 2021 Jun 3.

School of Materials Science and Engineering, Shandong University, Jinan 250061, PR China. Electronic address:

Absorbing materials can convert electromagnetic wave (EMW) energy into heat and other energy and dissipate it. Carbon materials can attenuate EMW by generating large conduction losses due to their high conductivity. The introduction of low dielectric materials can improve impedance matching caused by high conductivity. However, the density of materials compounded with carbon materials is too large, which affects the overall density of composite materials. Therefore, this problem is solved by matching melamine foam with ZIF-67. As an ultra-light material, the melamine foam-based carbon material can significantly reduce the density of composite materials, and its developed three-dimensional structure can cause multiple scattering of EMW. The large specific surface area and evenly distributed metal oxides obtained after annealing of ZIF-67 can provide ultra-low-density carbon materials and abundant interfacial polarization to further attenuate EMW. So far, the methods of self-growing materials on the surface of melamine foam have not been reported. We prepared a 500 nm CoO nanosheet/carbon foam (CF) composite material coated on the surface by a two-step method. The sample had a maximum reflection loss of -46.58 dB at 10.72 GHz, and an effective absorption bandwidth (EAB) of 5.4 GHz. This research provides a new idea for the growth of porous materials on the surface of melamine foam-based carbon materials.
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http://dx.doi.org/10.1016/j.jcis.2021.05.184DOI Listing
June 2021

The mitochondrial genome of (Diptera: Rhagionidae).

Mitochondrial DNA B Resour 2021 Jun 3;6(7):1837-1839. Epub 2021 Jun 3.

Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China.

The mitochondrial genome of Yang et Yang, 1997 was sequenced, the new representative of the family Rhagionidae. The complete mitochondrial genome is 16,430 bp totally, which consists of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and one non-coding control region. The nucleotide composition is biased toward A and T, accounting for 77.9% of the total. All genes have the conservational arrangement like other published species of Rhagionidae. Bayesian inference analysis strongly supported the monophyly of Rhagionidae and sister relationship between Coenomyiidae and Xylophagidae. The phylogenetic relationship among families of Tabanomorpha is very clear: (Athericidae + Tabanidae) + (Rhagionidae + (Coenomyiinae + Xylophagidae)).
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http://dx.doi.org/10.1080/23802359.2021.1934166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183545PMC
June 2021

Comparative Studies on Volatile Oil Components Derived from Integrated Processing Technology of Origin and Traditional Cutting Processing Technology of Moslae Herba and Their Effects on Rats With Lung-Yin Deficiency.

Evid Based Complement Alternat Med 2021 25;2021:5557222. Epub 2021 May 25.

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang, China.

This study aimed to examine the components of the volatile oil derived from the integration processing technology of origin (IPTO) of Moslae Herba (MH) and their effects on the treatment of rats with Lung-Yin deficiency. IPTO was compared with the traditional cutting processing technology (TCPT) to provide a feasible basis. The chemical constituents of the volatile oil were identified by gas chromatography-mass spectrometry (GC-MS). The Lung-Yin deficiency model was established by subjecting the animals to smoke and administering them with thyroid tablet suspension. The rats were randomly divided into six groups: control, model, Tween-80, positive, IPTO, and TCPT. After 30 days of intragastric administration, the macroscopic signs of rats and pathological morphology of the lung tissues were observed by the hematoxylin and eosin (H & E) staining method. The positive expression of the tumor necrosis factor (TNF-) was observed by an immunohistochemical method. The levels of cholesterol (CHO), albumin (ALB), total protein (TP), blood urea nitrogen (BUN), interleukin-1 (IL-1), interleukin-1beta (IL-1), cyclic adenosine monophosphate (cAMP), and cyclic guanosine monophosphate (cGMP) were detected in the serum of rats. A total of 42 and 37 components were identified from the volatile oils of IPTO and TCPT, respectively. Among them, the contents of carvacrol and thymol were higher in IPTO. The volatile oil of MH derived from IPTO and TCPT could improve the general signs and autonomous activities of the rats with Lung-Yin deficiency; increase the contents of cGMP, TP, and ALB; and decrease the anal temperature of the rats, the contents of IL-1, CHO, and BUN in serum, the ratio of cAMP to cGMP, and the average optical density of TNF- in their lung tissues. The findings indicated that IPTO was more efficient than TCPT. Its therapeutic effect might be associated with the change in TNF- expression; the increase in cGMP, TP, and ALB contents; and the decrease in IL-1, CHO, and BUN levels, laying the foundation for the clinical development and application of MH.
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http://dx.doi.org/10.1155/2021/5557222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172294PMC
May 2021

Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions.

Plant Cell 2021 Jun 12. Epub 2021 Jun 12.

School of Life Sciences & Institute of Plant and Food Science & Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.

DNA methylation in the non-CG context is widespread in the plant kingdom and abundant in mammalian tissues such as the brain and pluripotent cells. Non-CG methylation in Arabidopsis thaliana is coordinately regulated by DOMAINS REARRANGED METHYLTRANSFERASE (DRM) and CHROMOMETHYLASE (CMT) proteins but has yet to be systematically studied in major crops due to difficulties in obtaining genetic materials. Here, utilizing the highly efficient multiplex CRISPR-Cas9 genome-editing system, we created single- and multiple-knockout mutants for all nine DNA methyltransferases in rice (Oryza sativa) and profiled their whole-genome methylation status at single-nucleotide resolution. Surprisingly, the simultaneous loss of DRM2, CMT2, and CMT3 functions, which completely erases all non-CG methylation in Arabidopsis, only partially reduced it in rice. The regions that remained heavily methylated in non-CG contexts in the rice Os-dcc (Osdrm2/cmt2/cmt3a) triple mutant had high GC contents. Furthermore, the residual non-CG methylation in the Os-dcc mutant was eliminated in the Os-ddccc (Osdrm2/drm3/cmt2/cmt3a/cmt3b) quintuple mutant but retained in the Os-ddcc (Osdrm2/drm3/cmt2/cmt3a) quadruple mutant, demonstrating that OsCMT3b maintains non-CG methylation in the absence of other major methyltransferases. Our results showed that OsCMT3b is subfunctionalized to accommodate a distinct cluster of non-CG methylated sites at highly GC-rich regions in the rice genome.
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http://dx.doi.org/10.1093/plcell/koab162DOI Listing
June 2021

Relationship Between High-Sensitivity C-Reactive Protein and Long-Term Outcomes in Elderly Patients With 3-Vessel Disease.

Angiology 2021 Jun 10:33197211021195. Epub 2021 Jun 10.

34736Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China.

The prognostic value of high-sensitivity C-reactive protein (hsCRP) in complex coronary artery disease has not been fully established. We aimed to determine the association between hsCRP and long-term outcomes in elderly patients with 3-vessel disease (TVD). From April 2004 to February 2011, 3069 patients aged ≥65 years with TVD were consecutively enrolled and received medical treatment alone, percutaneous coronary intervention, or coronary artery bypass grafting. The patients were divided into 2 groups according to their hsCRP levels: <3.00 mg/L (62.1%) and ≥3.00 mg/L (37.9%). The mean age was 71 ± 4 years. The high hsCRP group had more risk factors and more frequently received conservative treatment than the low hsCRP group. During a median follow-up period of 6.2 years, elevated hsCRP was significantly associated with increased all-cause death (19.5% vs 29.6%, < .001), cardiac death (9.4% vs 15.2%, = .001), and major adverse cardiovascular and cerebrovascular events (34.1% vs 42.5%, = .001). Multivariable Cox regression analyses revealed that hsCRP was an independent predictor for all of these events. Combining hsCRP with Synergy between PCI with TAXUS and Cardiac Surgery score II further improved the predictive power of the score. The relationship between hsCRP and mortality was relatively consistent across subgroups. Overall, hsCRP could prove useful for risk prediction in elderly patients.
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http://dx.doi.org/10.1177/00033197211021195DOI Listing
June 2021

Oxygen defective titanate nanotubes induced by iron deposition for enhanced peroxymonosulfate activation and acetaminophen degradation: Mechanisms, water chemistry effects, and theoretical calculation.

J Hazard Mater 2021 May 27;418:126180. Epub 2021 May 27.

The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Peking University, Beijing 100871, China.

The large consumption of acetaminophen (APAP) worldwide and unsatisfactory treatment efficiencies by conventional wastewater treatment processes give rise to the seeking of new technology for its effective removal. Herein, we proposed a facile one-step hydrothermal method to synthesize defective iron deposited titanate nanotubes (Fe/TNTs) for peroxymonosulfate (PMS) activation and APAP degradation. The retarded first-order reaction rate of APAP degradation by Fe/TNTs was 5.1 times higher than that of neat TNTs. Characterizations indicated iron deposition effectively induced oxygen vacancies and Ti, facilitating the electrical conductivity and PMS binding affinity of Fe/TNTs. Besides, oxygen vacancies could act as an electron mediator through PMS activation by iron. Moreover, the formation of Fe-O-Ti bond facilitated the synergistic redox coupling between Fe and Ti, further enhancing the PMS activation. SO was the major radical, causing C-N bond cleavage and decreasing the overall toxicity. In contrast, APAP degradation by neat TNTs-PMS system mainly works through nonradical reaction. The Fe/TNTs activated PMS showed desired APAP removal under mild water chemistry conditions and good reusability. This work is expected to expand the potential application of titanate nanomaterials for PMS activation, and shed light on facile synthesis of oxygen defective materials for sulfate-radical-based advanced oxidation processes.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126180DOI Listing
May 2021

Zoledronic Acid in Osteoporotic Vertebral Compression Fractures Treated With Percutaneous Kyphoplasty: A Meta-Analysis.

Front Surg 2021 21;8:668551. Epub 2021 May 21.

Department of Traumatic Orthopedics, Tianjin Hospital, Tianjin, China.

We performed a meta-analysis to evaluate the efficacy and safety of zoledronic acid combined with percutaneous kyphoplasty (PKP) in treating osteoporotic vertebral compression fractures (OVCFs). Eligible scientific articles published prior to July 2020 were retrieved from the PubMed, Springer, ScienceDirect, and Cochrane Library databases. The statistical analysis was performed with RevMan 5.1. Three randomized controlled trials (RCTs) and 2 non-RCTs met the inclusion criteria. The present meta-analysis revealed that zoledronic acid combined with PKP is associated with a higher BMD, a better quality of life, less severe low back pain, and fewer additional vertebral body fractures than is percutaneous vertebral augmentation alone. Compared with PKP only, zoledronic acid combined with percutaneous vertebral augmentation is beneficial for OVCFs.
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http://dx.doi.org/10.3389/fsurg.2021.668551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176012PMC
May 2021

Plasma lipidome is dysregulated in Alzheimer's disease and is associated with disease risk genes.

Transl Psychiatry 2021 Jun 7;11(1):344. Epub 2021 Jun 7.

Centre for Healthy Brain Ageing (CHeBA), School of Psychiatry, University of New South Wales, Sydney, Australia.

Lipidomics research could provide insights of pathobiological mechanisms in Alzheimer's disease. This study explores a battery of plasma lipids that can differentiate Alzheimer's disease (AD) patients from healthy controls and determines whether lipid profiles correlate with genetic risk for AD. AD plasma samples were collected from the Sydney Memory and Ageing Study (MAS) Sydney, Australia (aged range 75-97 years; 51.2% male). Untargeted lipidomics analysis was performed by liquid chromatography coupled-mass spectrometry (LC-MS/MS). We found that several lipid species from nine lipid classes, particularly sphingomyelins (SMs), cholesterol esters (ChEs), phosphatidylcholines (PCs), phosphatidylethanolamines (PIs), phosphatidylinositols (PIs), and triglycerides (TGs) are dysregulated in AD patients and may help discriminate them from healthy controls. However, when the lipid species were grouped together into lipid subgroups, only the DG group was significantly higher in AD. ChEs, SMs, and TGs resulted in good classification accuracy using the Glmnet algorithm (elastic net penalization for the generalized linear model [glm]) with more than 80% AUC. In general, group lipids and the lipid subclasses LPC and PE had less classification accuracy compared to the other subclasses. We also found significant increases in SMs, PIs, and the LPE/PE ratio in human U251 astroglioma cell lines exposed to pathophysiological concentrations of oligomeric Aβ. This suggests that oligomeric Aβ plays a contributory, if not causal role, in mediating changes in lipid profiles in AD that can be detected in the periphery. In addition, we evaluated the association of plasma lipid profiles with AD-related single nucleotide polymorphisms (SNPs) and polygenic risk scores (PRS) of AD. We found that FERMT2 and MS4A6A showed a significantly differential association with lipids in all lipid classes across disease and control groups. ABCA7 had a differential association with more than half of the DG lipids (52.63%) and PI lipids (57.14%), respectively. Additionally, 43.4% of lipids in the SM class were differentially associated with CLU. More than 30% of lipids in ChE, PE, and TG classes had differential associations with separate genes (ChE-PICALM, SLC24A4, and SORL1; PE-CLU and CR1; TG-BINI) between AD and control group. These data may provide renewed insights into the pathobiology of AD and the feasibility of identifying individuals with greater AD risk.
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http://dx.doi.org/10.1038/s41398-021-01362-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180517PMC
June 2021

Anti-glial fibrillary acidic protein antibodies as a cause of reversible splenial lesion syndrome (RESLES): a case report.

Neurol Sci 2021 Jun 5. Epub 2021 Jun 5.

Department of Neurology, West China Hospital, Sichuan University, No.37 Guoxue Road, Chengdu, 610041, Sichuan, China.

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http://dx.doi.org/10.1007/s10072-021-05376-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179072PMC
June 2021

Multi-omics analysis of brain tissue metabolome and proteome reveals the protective effect of gross saponins of Tribulus terrestris L. fruit against ischemic stroke in rat.

J Ethnopharmacol 2021 Jun 1;278:114280. Epub 2021 Jun 1.

Key Laboratory of Medicinal Materials, Jilin Academy of Chinese Medicine Sciences, Changchun, 130021, China. Electronic address:

Ethnopharmacological Relevance: Gross Saponins of Tribulus terrestris L. Fruit (GSTTF) has been reported to have a protective effect against ischemic stroke, but the related mechanism is complex and still not fully investigated.

Aim Of The Study: The combination of metabolomics and proteomics approach was applied to reveal the mechanisms of GSTTF in treating ischemic stroke.

Materials And Methods: The metabolite and protein changes in brain tissue were analyzed by the LC-MS-based untargeted metabolomics method and tandem mass tags (TMT)-based quantitative proteomics technology. The multivariate statistical analysis and protein-protein interaction (PPI) analysis were conducted to screen out the biomarkers, and their related pathway was further investigated by the joint pathway analysis.

Results: A total of 110 metabolites and 359 differential proteins, which were mainly associated with complement and coagulation cascades, sphingolipid metabolism, glycerophospholipid metabolism, glutathione metabolism, and platelet activation, etc. were screened out from the rat brain tissue. The PPI network exhibited that the protein F2, Fga, Fgb, Fgg, Plg, and C3, which are greatly involved in the complement and coagulation cascades, have a relatively high connectivity degree, indicating their importance in the process of middle cerebral artery occlusion (MCAO). The GSTTF exerted a protective effect against MCAO via modulating multiple proteins on this pathway. Moreover, F2 played a key role during the protective process and worth to be further investigated due to it has been reported as one of the therapeutic targets of ischemic stroke.

Conclusion: The present study could improve the understanding of the potential therapeutic mechanism of GSTTF against ischemic stroke.
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http://dx.doi.org/10.1016/j.jep.2021.114280DOI Listing
June 2021

Does Overfilling Smooth Inflatable Saline-Filled Breast Implants Decrease the Deflation Rate? Experience with 4761 Augmentation Mammaplasty Patients.

Aesthetic Plast Surg 2021 Jun 3. Epub 2021 Jun 3.

Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.

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http://dx.doi.org/10.1007/s00266-021-02380-7DOI Listing
June 2021

The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori.

Arch Insect Biochem Physiol 2021 Jun 1:e21823. Epub 2021 Jun 1.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

The 30 K proteins are the major silkworm hemolymph proteins and are involved in a variety of physiological processes, such as nutrient and energy storage, embryogenesis, immune response, and inhibition of apoptosis. The Bm30K-15 protein is one of the 30 K proteins and is abundant in the hemolymph of fifth instar silkworm larva. We previously found that the Bm30K-15 protein can be acetylated. In the present study, we found that acetylation can improve the protein stability of Bm30K-15. Further exploration confirmed that the increase in protein stability by acetylation was caused by competition between acetylation and ubiquitination. In summary, these findings aim to provide insight into the effect of acetylation modification on the protein level and stability of the Bm30K-15 and the possible molecular mechanism of its existence in silkworm, Bombyx mori.
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http://dx.doi.org/10.1002/arch.21823DOI Listing
June 2021

A Genetic Study of Cerebral Atherosclerosis Reveals Novel Associations with and CNOT3.

Genes (Basel) 2021 May 26;12(6). Epub 2021 May 26.

Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA.

Cerebral atherosclerosis is a leading cause of stroke and an important contributor to dementia. Yet little is known about its genetic basis. To examine the association of common single nucleotide polymorphisms with cerebral atherosclerosis severity, we conducted a genomewide association study (GWAS) using data collected as part of two community-based cohort studies in the United States, the Religious Orders Study (ROS) and Rush Memory and Aging Project (MAP). Both studies enroll older individuals and exclude participants with signs of dementia at baseline. From our analysis of 1325 participants of European ancestry who had genotype and neuropathologically assessed cerebral atherosclerosis measures available, we found a novel locus for cerebral atherosclerosis in . The locus comprises eight SNPs, including two independent significant SNPs: rs6664221 ( = -0.27, 95% CI = (-0.35, -0.19), = 1.29 × 10) and rs10881463 ( = -0.20, 95% CI = (-0.27, -0.13), = 3.40 × 10). We further found that the SNPs may influence cerebral atherosclerosis by regulating brain protein expression of CNOT3. CNOT3 is a subunit of CCR4-NOT, which has been shown to be a master regulator of mRNA stability and translation and an important complex for cholesterol homeostasis. In summary, we identify a novel genetic locus for cerebral atherosclerosis and a potential mechanism linking this variation to cerebral atherosclerosis progression. These findings offer insights into the genetic effects on cerebral atherosclerosis.
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http://dx.doi.org/10.3390/genes12060815DOI Listing
May 2021

Neutral Detergent Cellulase Method and Chemical Composition to Predict Fermentable Organic Matter of Roughages.

Animals (Basel) 2021 May 28;11(6). Epub 2021 May 28.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

fermentable organic matter (FOM) reflects the energy production and the potential of rumen's microbial protein synthesis. However, the method with fistulated animals for FOM measurement compromises animal welfare and is laborious as well as expensive. Although the alternative nylon bag technique has been widely used, it is also costly and requires rumen liquor. Therefore, the present study was performed to compare the nylon bag technique with the neutral detergent cellulase (NDC) method or chemical composition to estimate FOM of roughages. For this purpose, we selected 12 roughages, including six each from forages and crop residues. Our results have shown the strong correlation equations between FOM and FOM of forages (n = 6; R = 0.79), crop residues (n = 6; R = 0.80), and roughages (n = 12; R = 0.84), respectively. Moreover, there were also strong correlations between the chemical composition of roughages and FOM (n = 12; R = 0.84-0.93) or FOM (n = 12; R = 0.79-0.89). In conclusion, the NDC method and chemical composition were alternatives to nylon bag technique for predicting FOM of roughages in the current experiment.
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http://dx.doi.org/10.3390/ani11061594DOI Listing
May 2021

Effect of Hybrid Type on Fermentation and Nutritional Parameters of Whole Plant Corn Silage.

Animals (Basel) 2021 May 28;11(6). Epub 2021 May 28.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

This study was designed to evaluate the effect of hybrid type on the fermentation and nutritional parameters of whole-plant corn silage (dual-purpose and silage-specific corn). For this purpose, the two corn hybrid types were harvested at the one-half to three-fourths milk line and ensiled in fermentation bags (50 × 80 cm) for 60 day. Our results demonstrated that the ratio of lactic acid to acetic acid ( = 0.004), propionic acid ( < 0.001), Flieg point ( < 0.001), ether extract ( = 0.039), starch ( < 0.001), milk-per-ton index ( < 0.005), net energy for lactation ( = 0.003), total digestible nutrients ( < 0.001), neutral detergent soluble fiber ( =0.04), and in situ dry matter digestibility (TDMD) ( < 0.001) were higher in dual-purpose corn silage, while the pH ( = 0.014), acetic acid ( = 0.007), the ratio of ammonia nitrogen to total nitrogen ( = 0.045), neutral detergent fiber ( < 0.001), acid detergent fiber ( < 0.001), acid detergent lignin ( < 0.001), dry matter yield per ha ( < 0.001), milk-per-acre index ( = 0.003), available neutral detergent fiber ( < 0.001), and unavailable neutral detergent fiber ( < 0.001) were higher in silage-specific corn silage. Based on our analysis, we concluded that under favourable production conditions for whole-plant corn silage, the nutritive value per unit was higher in dual-purpose corn while biomass yield and nutrient value per ha were higher in silage-specific corn.
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http://dx.doi.org/10.3390/ani11061587DOI Listing
May 2021

Development and application of novel electrophilic warheads in target identification and drug discovery.

Biochem Pharmacol 2021 May 29;190:114636. Epub 2021 May 29.

School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China; MOE Key Laboratory of Tumor Molecular Biology, Jinan University, Guangzhou, China. Electronic address:

Nucleophilic amino acids play important roles in maintenance of protein structure and function, covalent modification of such amino acid residues by therapeutic agents is an efficient way to treat human diseases. Most of current clinical drugs are structurally limited to α,β-unsaturated amide as an electrophilic warhead. To alleviate this issue, many novel electrophiles have been developed in recent years that can covalently bind to different amino acid residues and provides a unique way to interrogate proteins, including "undruggable" targets. With an activity-based protein profiling (ABPP) approach, the activity and functionality of a protein and its binding sites can be assessed. This facilitates an understanding of protein function, and contributes to the discovery of new druggable targets and lead compounds. Meanwhile, many novel inhibitors bearing new reactive warhead were developed and displayed remarkable pharmaceutical properties. In this perspective, we have reviewed the recent remarkable progress of novel electrophiles and their applications in target identification and drug discovery.
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http://dx.doi.org/10.1016/j.bcp.2021.114636DOI Listing
May 2021

The immediate trends in atrial electrical remodeling for paroxysmal atrial fibrillation across different modes of catheter ablation.

Clin Cardiol 2021 Jun 1. Epub 2021 Jun 1.

Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Catheter ablation has emerged as a major strategy for paroxysmal atrial fibrillation (PAF). Atrial electrical remodeling (AER) plays a critical role in the recurrence of PAF after ablation.

Hypothesis: To characterize the immediate trends of AER during ablations in patients with PAF, and assess the relationship between immediate trends and recurrence.

Methods: We performed this prospective observational study of 135 patients to investigate AER following three ablation modes: radiofrequency ablation (RFA), cryoablation (CA) and 3D mapping-guided cryoablation (3D-CA). The atrial effective refractory period (AERP) and atrial conduction time (ACT) were measured via electrophysiology before and immediately after ablation, and P-wave indices were measured via electrocardiography before and within 24 h after ablation. Follow-up visits were conducted for at least 1 year or until relapse.

Results: Different approaches of ablation caused a fairly significant increase in the shortest P-wave duration and AERP in both the proximal coronary sinus (PCS) and distal coronary sinus (DCS) but caused a shortened P-wave dispersion. No different effect was found at the AERP among the three modes. Compared to patients who received CA, among patients who received RFA, a significant reduction in total ACT and right ACT was seen. Statistically, there was a weakly positive association between changes in total ACT and early recurrence.

Conclusions: Injury during ablation for PAF was associated with an increase in the AERP but not in the ACT. Total ACT and right ACT were shorter after RFA than after CA. The increase in total ACT were slightly predictive of early recurrence.
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http://dx.doi.org/10.1002/clc.23617DOI Listing
June 2021

All five COVID-19 outbreaks during epidemic period of 2020/2021 in China were instigated by asymptomatic or pre-symptomatic individuals.

J Biosaf Biosecur 2021 Jun 21;3(1):35-40. Epub 2021 May 21.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province 030001, PR China.

Introduction: The significance of asymptomatic or pre-asymptomatic individuals in driving the COVID-19 epidemic in China or other countries remains uncertain.

Method: We collected and analyzed all the epidemiologic and virological diagnostic details of the infected individuals released by public health authorities and reiterated every episode of outbreak on a timeline. All individuals associated with the five outbreaks had tested positive for SARS-CoV-2 infection.

Results: In this study, all five COVID-19 outbreaks reported in China since October 2020 were analyzed. The Kashgar outbreak in Xinjiang province came into light for the first time on October 22, 2020. However, it was initiated before October 11, 2020, by a local asymptomatic import and export worker, who was infected at the working place. Subsequently, his wife caught the infection, which led to 430 more infections reported in the outbreak. The Beijing outbreak with 41 cases was noticed for the first time on December 22, 2020. However, our analysis revealed that it was initiated by an asymptomatic individual from Indonesia on December 10, 2020. The Shenyang outbreak, with 38 cases, noticed for the first time on December 23, 2020, was initiated by a pre-symptomatic individual from South Korea on December 13, 2020.

Conclusion: The asymptomatic or pre-symptomatic individuals during the asymptomatic period were unsuspectingly infected by SARS-CoV-2, and unintentionally transmitted the virus to a large number of people. These findings suggest that early detection of asymptomatic or pre-symptomatic individuals is of critical importance in preventing future outbreaks or epidemics.
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http://dx.doi.org/10.1016/j.jobb.2021.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139231PMC
June 2021

Xanthoceraside administration produces significant antidepressant effects in mice through activation of the hippocampal BDNF signaling pathway.

Neurosci Lett 2021 Jul 29;757:135994. Epub 2021 May 29.

Department of Pharmacology, Pharmacy College, Nantong University, Nantong 226001, Jiangsu, China; Provincial Key Laboratory of Inflammation and Molecular Drug Target, Nantong 226001, Jiangsu, China. Electronic address:

Current available antidepressants have various adverse reactions and slow pharmacodynamics, so it is necessary to find novel antidepressants for effective treatment. Xanthoceraside (XAN), a novel triterpenoid saponin extracted from the fruit husks of Xanthoceras sorbifolium Bunge, has anti-amnesic and neuroprotective properties. The purpose and significance of this study is to assess whether XAN has antidepressant effects in mice using the forced swim test (FST), tail suspension test (TST) and chronic unpredictable mild stress (CUMS) model of depression. The effects of XAN treatment on the hippocampal brain-derived neurotrophic factor (BDNF) signaling pathway and neurogenesis were examined. The antidepressant mechanism of XAN was explored using a BDNF inhibitor (K252a) and an anti-BDNF antibody. It was found that XAN administration significantly reversed the depressive-like behaviors of CUMS-treated mice. XAN treatment also significantly prevented the decreasing effects of CUMS on the hippocampal BDNF signaling and neurogenesis. The antidepressant effects of XAN in mice were blocked by both administration of K252a and anti-BDNF antibody. Collectively, these findings indicate that XAN possesses antidepressant effects in mice which are mediated by activation of hippocampal BDNF signaling pathway, thus providing the first evidence that XAN can be a potential antidepressant candidate.
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http://dx.doi.org/10.1016/j.neulet.2021.135994DOI Listing
July 2021

Impact of NSLAB on Kazakh cheese flavor.

Food Res Int 2021 Jun 23;144:110315. Epub 2021 Mar 23.

Institute of Agro-products Processing Science and Technology, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, PR China.

Xinjiang is a multiethnic region of China. Traditionally, most ethnic minorities are known to produce and consume cheese. Nomadic people have been reported to use lactic acid bacteria (LAB) for decades to produce fermented dairy products as part of a balanced diet. Non-starter LAB (NSLAB) contribute to different degrees of ripening, depending on the cheese variety. In the present study, we screened three types of NSLAB with good proteolysis and autolytic abilities from traditional Kazakh cheese: Pediococcus acidilactici R3-5, Staphylococcus epidermidis R4-2, and Lactobacillus rhamnosus R9-6. A control (no NSLAB) was also included, resulting in four distinct types of cheese samples. We used gas chromatography-mass spectrometry and the electronic nose system to identify volatile compounds and analyze the effect of NSLAB on cheese flavor at the ripening stage. The physicochemical indicators changed significantly during the ripening of Kazakh cheese. Compared with the control, the protein content, free fatty acid content, pH, flavor compounds, and odor profiles of the test cheeses were significantly different. The major chemical differences among cheeses were the synthesis of some key volatile components (ethyl caprylate, ethyl caprate, myristyl carbonate, capric acid, caprylic acid, nonanal, and benzyl alcohol). NSLAB can be used as an adjunct starter to make Kazakh cheese and the use of NSLAB affected the cheese flavor quality positively.
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http://dx.doi.org/10.1016/j.foodres.2021.110315DOI Listing
June 2021

SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Immunity 2021 06 9;54(6):1304-1319.e9. Epub 2021 May 9.

Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA.

Despite mounting evidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) engagement with immune cells, most express little, if any, of the canonical receptor of SARS-CoV-2, angiotensin-converting enzyme 2 (ACE2). Here, using a myeloid cell receptor-focused ectopic expression screen, we identified several C-type lectins (DC-SIGN, L-SIGN, LSECtin, ASGR1, and CLEC10A) and Tweety family member 2 (TTYH2) as glycan-dependent binding partners of the SARS-CoV-2 spike. Except for TTYH2, these molecules primarily interacted with spike via regions outside of the receptor-binding domain. Single-cell RNA sequencing analysis of pulmonary cells from individuals with coronavirus disease 2019 (COVID-19) indicated predominant expression of these molecules on myeloid cells. Although these receptors do not support active replication of SARS-CoV-2, their engagement with the virus induced robust proinflammatory responses in myeloid cells that correlated with COVID-19 severity. We also generated a bispecific anti-spike nanobody that not only blocked ACE2-mediated infection but also the myeloid receptor-mediated proinflammatory responses. Our findings suggest that SARS-CoV-2-myeloid receptor interactions promote immune hyperactivation, which represents potential targets for COVID-19 therapy.
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http://dx.doi.org/10.1016/j.immuni.2021.05.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106883PMC
June 2021

Branching development of early post-implantation human embryonic-like tissues in 3D stem cell culture.

Biomaterials 2021 May 18;275:120898. Epub 2021 May 18.

Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA. Electronic address:

Human embryonic stem cells (hESCs) have the intrinsic capacity to self-organize and generate patterned tissues. In vitro models that coax hESCs to form embryonic-like structures by modulating physical environments and priming with chemical signals have become a powerful tool for dissecting the regulatory mechanisms underlying early human development. Here we present a 3D suspension culture system of hESCs that can generate post-implantation, pre-gastrulation embryonic-like tissues in an efficient and controllable manner. The efficiency of the development of asymmetric tissues, which mimic the post-implantation, pre-gastrulation amniotic sac, was about 50% in the 3D suspension culture. Quantitative imaging profiling and unsupervised trajectory analysis revealed that hESC aggregates first entered into a transitional stage expressing Brachyury (or T), before their development branched into different paths to develop into asymmetric embryonic-like tissues, amniotic-like tissues, and mesodermal-like tissues, respectively. Moreover, the branching developmental trajectory of embryonic-like structures was affected by the initial cell seeding density or cluster size of hESCs. A higher percentage of amniotic-like tissues was observed under a small initial cell seeding density of hESCs. Conversely, a large initial cell seeding density of hESCs promoted the development of mesodermal-like tissues. Intermediate cell seeding densities of hESCs in the 3D suspension culture promoted the development of asymmetric embryonic-like tissues. Our results suggest that hESCs have the intrinsic capability to sense the initial cell population size, which in turn regulates their differentiation and self-organization into different embryonic-like tissues. Our 3D suspension culture thus provides a promising experimental tool to study the interplay between tissue topology and self-organization and progressive embryonic development using in vitro hESC-based models.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120898DOI Listing
May 2021

The Abuse Characteristics of Amphetamine-Type Stimulants in Patients Receiving Methadone Maintenance Treatment and Buprenorphine Maintenance Treatment.

Drug Des Devel Ther 2021 18;15:2109-2116. Epub 2021 May 18.

Department of Basic Medicine, Ningbo University School of Medicine, Ningbo, 315211, People's Republic of China.

Objective: The purpose of this study was to retrospectively investigate the abuse characteristics of amphetamine-type stimulants (ATS) in patients receiving methadone maintenance treatment (MMT) and buprenorphine maintenance treatment (BMT).

Methods: A total of 58 MMT and 51 BMT patients abusing ATS were recruited from the drug maintenance treatment clinic of Ningbo Addiction Research and Treatment Center from January 2018 to December 2019. They were assessed using the amphetamine abuse questionnaire (AAQ), addiction severity index (ASI) and Barratt impulsiveness scale (BIS). Moreover, 40 MMT control patients, 40 BMT control patients and 20 healthy controls were also assessed using the BIS. All information was collected using the amphetamine abuse questionnaire (AAQ), Chinese version of addiction severity index (ASI-C) and Chinese version of Barratt impulsiveness scale (BIS-C) conducted by qualified psychologists.

Results: The interval of amphetamine use in the MMT group was shorter than the BMT group ( < 0.05). The drug use subscale score of ASI was higher in the MMT group than the BMT group ( < 0.05). The respective and total scores of attentional impulsiveness, motor impulsiveness and non-planning impulsiveness in BIS in the MMT group were all higher than the MMT control group ( < 0.05). The scores of motor impulsiveness and non-planning impulsiveness in the BMT group were higher than the BMT control group ( < 0.05). The respective and total scores in BIS in the MMT control group and the BMT control group were all higher than those in the healthy controls.

Conclusion: The patients showing amphetamine abuse in maintenance therapy had a greater impulsiveness than those having other simple maintenance treatments, and patients under MMT may be more addicted to amphetamines in comparison with those having BMT.
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http://dx.doi.org/10.2147/DDDT.S305226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140901PMC
May 2021

One-pot and Shape-controlled Synthesis of Organic Cages.

Angew Chem Int Ed Engl 2021 May 25. Epub 2021 May 25.

Shanghai University, Chemistry, 99 Shangda Road, 200443, Shanghai, CHINA.

Organic cages are fascinating synthetic goals because of their well-defined three-dimensional cavities, excellent stability and accessible post-modification. However, the synthesis is normally realized by fragment coupling approach in low yields. Herein, we report one-pot, gram-scale and shape-controlled synthesis of two covalent organic cages (box-shaped [4]cage and triangular prism-shaped [2]cage) in yields of 46% and 52%, involving direct condensation of triangular 1,3,5-tris(2,4-dimethoxyphenyl)benzene monomer with paraformaldehyde and isobutyraldehyde, respectively. The resulted cage can convert into high-yielding per-hydroxylated analogue which exhibits great potential for further modification. Moreover, [2]cage can be utilized as gas chromatographic stationary phase for high-resolution separation of benzene/cyclohexane and toluene/methylcyclohexane. By changing the central moiety of the triangular monomer and/or aldehyde, this synthetic method would have the potential to be a general strategy to access diverse cages with tunable shape, size, and electronic properties.
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http://dx.doi.org/10.1002/anie.202104875DOI Listing
May 2021

Bacterial and archaeal cytoskeletons.

Authors:
Yue Liu Jan Löwe

Curr Biol 2021 May;31(10):R542-R546

MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address:

All living cells depend on the intricate organization of molecular components in space and time. Although this notion was historically based on eukaryotic cells, with their structured intracellular architecture and cellular morphologies, it is now recognized that prokaryotes (that is, bacteria and archaea) also possess complex structures. A cytoskeleton is a network of intracellular protein filaments that play a structural or mechanical role (such as scaffolding, pushing, or pulling) in the spatiotemporal organization of cellular processes. Polymerization of protein monomers in a roughly linear fashion into filaments represents an effective means to establish long-range spatial order by bridging the gap between nanometer-sized molecules and micron-sized cells. It is now evident that bacteria and archaea possess numerous kinds of cytoskeletal proteins, including prokaryotic homologues of the eukaryotic actins, tubulins, and intermediate filaments, as well as other types that have been found primarily or exclusively in prokaryotes (Table 1). Understanding the diverse functions and mechanisms of the rapidly growing universe of prokaryotic cytoskeletal proteins will not only advance prokaryotic cell biology and reveal evolutionary principles, but also open up new avenues for the development of anti-microbial agents, de novo protein design, and the construction of minimal and synthetic cells.
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http://dx.doi.org/10.1016/j.cub.2021.03.011DOI Listing
May 2021

Electrochemically reduced phytic acid-doped TiO nanotubes for the efficient electrochemical degradation of toxic pollutants.

J Hazard Mater 2021 07 6;414:125600. Epub 2021 Mar 6.

School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China; Nanjing University & Yancheng Academy of Environmental Protection Technology and Engineering, Yancheng 210009, PR China. Electronic address:

Element-doped TiO nanotube arrays (TNAs) with optimized active sites provide an effective approach for significantly improving electrocatalytic performance. The challenges in such construction mainly include selection of green dopant and control of active sites. Herein, we present phytic acid as a phosphorus source for P-doped TNAs. An oxygen vacancy (Ov) and P co-doped TNAs (P-TiO) was prepared as an electrochemical oxidation anode. P-TiO exhibits excellent degradation activity due to the formation of Ti-O-P bonds and generation of Ov. P-doping was beneficial in improving the oxygen evolution potential of the electrode, which would be benefit for electrocatalytic degradation of pollutants. Using the P-TiO anode with a current density of 10 mA/cm for tetracycline degradation, after a 3 h treatment, the removal rate, chemical oxygen demand and total organic carbon removal rates were 100%, 90.32% and 76.60%, respectively. The P-TiO also has excellent degradation performance for phenol, hydroquinone, p-nitrophenol and metronidazole.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125600DOI Listing
July 2021

Peroxiredoxin II with dermal mesenchymal stem cells accelerates wound healing.

Aging (Albany NY) 2021 05 24;13(10):13926-13940. Epub 2021 May 24.

College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, P.R. China.

Peroxiredoxin II (Prx II) is involved in proliferation, differentiation, and aging in various cell types. However, Prx II-mediated stem cell regulation is poorly understood. Here, dermal mesenchymal stem cells (DMSCs), cell-growth factor-rich conditioned medium from DMSCs (DMSC-CM), and DMSC-derived exosomes (DMSC-Exos) were used to explore the regulatory role of Prx II in DMSC wound healing. Following treatment, wound healing was significantly decelerated in Prx II DMSCs than in Prx II DMSCs. stimulation with 10 μM HO significantly increased apoptosis in Prx II DMSCs compared with Prx II DMSCs. The mRNA expression levels of EGF, b-FGF, PDGF-B, and VEGF did not significantly differ between Prx II and Prx II DMSCs. Fibroblasts proliferated comparably when treated with Prx II DMSC-CM or Prx II DMSC-CM. Wound healing was significantly higher in the Prx II DMSC-Exos-treated group than in the Prx II DMSCs-Exos-treated group. Moreover, microRNA (miR)-21-5p expression levels were lower and miR-221 levels were higher in Prx II DMSCs than in Prx II DMSCs. Therefore, our results indicate that Prx II accelerated wound healing by protecting DMSCs from reactive oxygen species-induced apoptosis; however, Prx II did not regulate cell/growth factor secretion. Prx II potentially regulates exosome functions via miR-21-5p and miR-221.
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http://dx.doi.org/10.18632/aging.202990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202850PMC
May 2021

The complete chloroplast genome sequence of Lévl.

Mitochondrial DNA B Resour 2021 May 12;6(5):1621-1622. Epub 2021 May 12.

Department of Medical College, Qinghai University, Xining, PR China.

is a medicinal plant distributed in northern China and has high economic and social value. In this study, we reported the complete chloroplast genome sequence of the plant. We determined that the length of the chloroplast genome of Lévl is found to be 155,787 bp and the GC content is 37.24%. The cp genome sequences contains 132 genes, including 37 tRNA genes, eight rRNA genes, and 87 mRNA genes, respectively. The genomic data can help the classification and evolution of Rubus plants, and provide a theoretical basis for the study of Lévl.
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http://dx.doi.org/10.1080/23802359.2021.1926354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118508PMC
May 2021

Microbiota and Tuberculosis: A Potential Role of Probiotics, and Postbiotics.

Front Nutr 2021 7;8:626254. Epub 2021 May 7.

Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China.

Tuberculosis (TB), caused by attacking the lungs and other organs, is one of the most common infectious disease worldwide. According to the WHO's 2020 report, a quarter of the world's population were infected with , and ~1.4 million people died of TB. Therefore, TB is a significant public health concern, which requires cost-effective strategies for prevention and treatment. The microbiota has been considered as a "forgotten organ" and a complex dynamic ecosystem, which plays a significant role in many physiological processes, and its dysbiosis is closely associated with infectious disease. Recently, a few studies have indicated associations between TB and microbiota. This review summarizes studies concerning the alterations of the gut and respiratory microbiota in TB, and their relationship with host susceptibility to infection, indicating that microbiota signatures in different stages in TB progression could be considered as biomarkers for TB diagnosis and control. In addition, the potential role of probiotics and postbiotics in TB treatment was discussed.
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http://dx.doi.org/10.3389/fnut.2021.626254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138307PMC
May 2021

TMT-based proteomic and bioinformatic analyses of human granulosa cells from obese and normal-weight female subjects.

Reprod Biol Endocrinol 2021 May 20;19(1):75. Epub 2021 May 20.

Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiao Tong University, 200025, Shanghai, China.

Background: Increasing evidence supports a relationship between obesity and either infertility or subfertility in women. Most previous omics studies were focused on determining if the serum and follicular fluid expression profiles of subjects afflicted with both obesity-related infertility and polycystic ovary syndrome (PCOS) are different than those in normal healthy controls. As granulosa cells (GCs) are essential for oocyte development and fertility, we determined here if the protein expression profiles in the GCs from obese subjects are different than those in their normal-weight counterpart.

Methods: GC samples were collected from obese female subjects (n = 14) and normal-weight female subjects (n = 12) who were infertile and underwent in vitro fertilization (IVF) treatment due to tubal pathology. A quantitative approach including tandem mass tag labeling and liquid chromatography tandem mass spectrometry (TMT) was employed to identify differentially expressed proteins. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were then conducted to interrogate the functions and pathways of identified proteins. Clinical, hormonal, and biochemical parameters were also analyzed in both groups.

Results: A total of 228 differentially expressed proteins were noted, including 138 that were upregulated whereas 90 others were downregulated. Significant pathways and GO terms associated with protein expression changes were also identified, especially within the mitochondrial electron transport chain. The levels of free fatty acids in both the serum and follicular fluid of obese subjects were significantly higher than those in matched normal-weight subjects.

Conclusions: In GCs obtained from obese subjects, their mitochondria were damaged and the endoplasmic reticulum stress response was accompanied by dysregulated hormonal synthesis whereas none of these changes occurred in normal-weight subjects. These alterations may be related to the high FFA and TG levels detected in human follicular fluid.
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http://dx.doi.org/10.1186/s12958-021-00760-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135161PMC
May 2021