Publications by authors named "Yue Li"

3,132 Publications

  • Page 1 of 1

Protein tyrosine phosphatase PTPN22 negatively modulates platelet function and thrombus formation.

Blood 2022 Jun 29. Epub 2022 Jun 29.

Blood Disease Institute, Xuzhou Medical University, Xuzhou, China.

Protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a protein tyrosine phosphatase and negatively regulates T cell signaling. However, whether it is expressed and functions in platelets remains unknown. Here we investigated the expression and role of PTPN22 in platelet function. We showed PTPN22 expression in both human and mouse platelets. Using PTPN22-/- mice, we demonstrated that PTPN22 deficiency significantly shortened tail-bleeding time and accelerated arterial thrombus formation without affecting venous thrombosis and the coagulation factor VIII and IX. Consistently, PTPN22-deficient platelets exhibited enhanced platelet aggregation, granules secretion, calcium mobilization, lamellipodia formation, spreading and clot retraction. Quantitative phosphoproteomic analysis revealed the significant difference of phosphodiesterase 5A (PDE5A) phosphorylation in PTPN22-deficient platelets compared to wild-type platelets after collagen-related peptide (CRP) stimulation, which was confirmed by increased PDE5A phosphorylation (Ser92) in CRP-treated PTPN22-deficient platelets, concomitant with reduced cGMP level and VASP phosphorylation (Ser157/239). In addition, PTPN22 interacted with phosphorylated PDE5A (Ser92) and dephosphorylated it in activated platelets. Moreover, purified PTPN22 but not mutant form (C227S) possesses intrinsic serine phosphatase activity. Furthermore, inhibition of PTPN22 enhanced human platelet aggregation, spreading, clot retraction and increased PDE5A phosphorylation (Ser92). In conclusion, our study demonstrates a novel role of PTPN22 in platelet function and arterial thrombosis, identifying new potential targets for future prevention of thrombotic or cardiovascular diseases.
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http://dx.doi.org/10.1182/blood.2022015554DOI Listing
June 2022

Intrinsically Disordered Protein Condensate-Modified Surface for Mitigation of Biofouling and Foreign Body Response.

J Am Chem Soc 2022 Jun 29. Epub 2022 Jun 29.

Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.

Mitigation of biofouling and the host's foreign body response (FBR) is a critical challenge with biomedical implants. The surface coating with various anti-fouling materials provides a solution to overcome it, but limited options in clinic and their potential immunogenicity drive the development of more alternative coating materials. Herein, inspired by liquid-liquid phase separation of intrinsically disordered proteins (IDPs) to form separated condensates in physiological conditions, we develop a new type of low-fouling biomaterial based on flexible IDP of FUS protein containing rich hydrophilic residues. A chemical structure-defined FUS IDP sequence tagged with a tetra-cysteine motif (IDP) was engineered and applied for covalent immobilization on various surfaces to form a uniform layer of protein tangles, which boosted strong hydration on surfaces, as revealed by molecular dynamics simulation. The IDP-coated surfaces displayed excellent performance in resisting adsorption of various proteins and adhesion of different cells, platelets, and bacteria. Moreover, the IDP-coated implants largely mitigated the host's FBR compared with bare implants and particularly outperformed PEG-coated implants in reducing collagen encapsulation. Thus, this novel low-fouling and anti-FBR strategy provides a potential surface coating material for biomedical implants, which will also shed light on exploring similar applications of other IDP proteins.
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http://dx.doi.org/10.1021/jacs.2c02677DOI Listing
June 2022

The impact of low intensity ultrasound on cells: Underlying mechanisms and current status.

Prog Biophys Mol Biol 2022 Jun 25. Epub 2022 Jun 25.

The First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China, Hengyang, Hunan, China; Institute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, China. Electronic address:

Low intensity ultrasound (LIUS) has been adopted for a variety of therapeutic purposes because of its bioeffects such as thermal, mechanical, and cavitation effects. The mechanism of impact and cellular responses of LIUS in cellular regulations have been revealed, which helps to understand the role of LIUS in tumor treatment, stem cell therapy, and nervous system regulation. The review summarizes the bioeffects of LIUS at the cellular level and its related mechanisms, detailing the corresponding theoretical basis and latest research in the study of LIUS in the regulation of cells. In the future, the design of specific LIUS-mediated treatment strategies may benefit from promising investigations which is hoped to provide encouraging therapeutic data.
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http://dx.doi.org/10.1016/j.pbiomolbio.2022.06.004DOI Listing
June 2022

Single-cell transcriptomic analysis identifies an immune-prone population in erythroid precursors during human ontogenesis.

Nat Immunol 2022 Jun 27. Epub 2022 Jun 27.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Nonimmune cells can have immunomodulatory roles that contribute to healthy development. However, the molecular and cellular mechanisms underlying the immunomodulatory functions of erythroid cells during human ontogenesis remain elusive. Here, integrated, single-cell transcriptomic studies of erythroid cells from the human yolk sac, fetal liver, preterm umbilical cord blood (UCB), term UCB and adult bone marrow (BM) identified classical and immune subsets of erythroid precursors with divergent differentiation trajectories. Immune-erythroid cells were present from the yolk sac to the adult BM throughout human ontogenesis but failed to be generated in vitro from human embryonic stem cells. Compared with classical-erythroid precursors, these immune-erythroid cells possessed dual erythroid and immune regulatory networks, showed immunomodulatory functions and interacted more frequently with various innate and adaptive immune cells. Our findings provide important insights into the nature of immune-erythroid cells and their roles during development and diseases.
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http://dx.doi.org/10.1038/s41590-022-01245-8DOI Listing
June 2022

Serum and urine angiopoietin-like protein 4 levels correlate with different degrees of dislipidemea and proteinuria in patients with primary nephrotic syndrome.

Eur J Intern Med 2022 Jun 24. Epub 2022 Jun 24.

Department of Nephrology, Guangzhou Women and Children's Medical Center, No. 318 Renmin Middle Road, Guangzhou 510623, China. Electronic address:

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http://dx.doi.org/10.1016/j.ejim.2022.06.016DOI Listing
June 2022

The liver X receptors agonist GW3965 attenuates depressive-like behaviors and suppresses microglial activation and neuroinflammation in hippocampal subregions in a mouse depression model.

J Comp Neurol 2022 Jun 27. Epub 2022 Jun 27.

Department of Physiology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.

Liver X receptors (LXRs) have recently been reported to be novel and potential targets for the reversal of depressive-like behaviors, but the mechanism remains unclear. Hippocampal neuroinflammation and impairment of the normal structure and function of microglia are closely associated with depression. To investigate the effects of LXRs agonist (GW3965) on neuroinflammation and microglia in the hippocampal formation of mice with chronic unpredictable stress (CUS)-induced depression, depressive-like behaviors were evaluated by behavioral tests, hippocampal LXRs gene expression were evaluated by qRT-PCR, the protein expression levels of interleukin-1β, tumor necrosis factor-α, inducible nitric oxide synthase, nuclear factor kappa B, and cluster of differentiation 206 were estimated by western blotting, modern stereological methods were used to precisely quantify the total number of microglia in each hippocampal subregion, and immunofluorescence was used to detect the density of activated microglia and the morphology of microglia. We found that GW3965 alleviated the depressive-like behavior induced by CUS, reversed the decrease in hippocampal LXRα and LXRβ induced by CUS, increased the protein expression of pro-inflammatory factors, and decreased the protein expression of antiinflammatory factors induced by CUS. Moreover, CUS intervention significantly increased the number of microglia in the CA1 region, CA2/3 region, and dentate gyrus and the density of activated microglia in the CA2/3 region and dentate gyrus and significantly decreased the endpoints of microglial branches and process length of microglia in the dentate gyrus, while 4 weeks of injections with GW3965 reversed these changes. These findings suggest that regulating the number, activated state, and morphology of microglia in hippocampal subregions might be an important basis for the antidepressant effects of LXRs.
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http://dx.doi.org/10.1002/cne.25380DOI Listing
June 2022

Improving Estimation of Winter Wheat Nitrogen Status Using Random Forest by Integrating Multi-Source Data Across Different Agro-Ecological Zones.

Front Plant Sci 2022 10;13:890892. Epub 2022 Jun 10.

MARA Key Laboratory for Crop System Analysis and Decision Making, Jiangsu Key Laboratory for Information Agriculture, National Engineering and Technology Center for Information Agriculture, MOE Engineering and Research Center for Smart Agriculture, Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing, China.

Timely and accurate estimation of plant nitrogen (N) status is crucial to the successful implementation of precision N management. It has been a great challenge to non-destructively estimate plant N status across different agro-ecological zones (AZs). The objective of this study was to use random forest regression (RFR) models together with multi-source data to improve the estimation of winter wheat ( L.) N status across two AZs. Fifteen site-year plot and farmers' field experiments involving different N rates and 19 cultivars were conducted in two AZs from 2015 to 2020. The results indicated that RFR models integrating climatic and management factors with vegetation index (R = 0.72-0.86) outperformed the models by only using the vegetation index (R = 0.36-0.68) and performed well across AZs. The Pearson correlation coefficient-based variables selection strategy worked well to select 6-7 key variables for developing RFR models that could achieve similar performance as models using full variables. The contributions of climatic and management factors to N status estimation varied with AZs and N status indicators. In higher-latitude areas, climatic factors were more important to N status estimation, especially water-related factors. The addition of climatic factors significantly improved the performance of the RFR models for N nutrition index estimation. Climatic factors were important for the estimation of the aboveground biomass, while management variables were more important to N status estimation in lower-latitude areas. It is concluded that integrating multi-source data using RFR models can significantly improve the estimation of winter wheat N status indicators across AZs compared to models only using one vegetation index. However, more studies are needed to develop unmanned aerial vehicles and satellite remote sensing-based machine learning models incorporating multi-source data for more efficient monitoring of crop N status under more diverse soil, climatic, and management conditions across large regions.
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http://dx.doi.org/10.3389/fpls.2022.890892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226625PMC
June 2022

Outer membrane vesicles derived from heatstroke-associated intestinal microbiota promote multiple organ injury in mice.

Microb Pathog 2022 Jun 23:105653. Epub 2022 Jun 23.

Department of Treatment, Center for Traumatic Injuries, The Third Affiliated Hospital of Southern Medical University, #183 Zhongshan Road West, Guangzhou, Guangdong, 510630, China; Academy of Orthopedics, Guangdong Province, Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, #183 Zhongshan Road West, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, 510630, China. Electronic address:

Multiple organ injury is a common issue in heatstroke (HS); however, the underlying pathogenesis remains unclear. As an early event in HS, intestinal injury is an active participant that drives organ injury. Outer membrane vesicles (OMVs), a group of vesicles shed by unbalanced intestinal microbiota as "danger signals," mediate different functional cargo transport in cells and modulate varying biological events in distant target cells. However, the role of OMVs in HS-mediated organ damage remains unclear. Therefore, this study examined OMV production in HS and explored the effect of regulating multiple organ injury. To construct a mouse model, animals were exposed to hyperthermia. OMVs from the intestinal microbiota of HS and control mice were extracted by standardized differential ultracentrifugation. Thereafter, OMVs were characterized and infused into recipient mice via the tail vein. Cl-amidine (a pan-peptidylarginine deiminase inhibitor and OMV production inhibitor) was injected intraperitoneally (2 mg/kg) 2 h before HS treatment, and the absorption of HS OMVs by different organs was tracked. The effect of OMVs on inducing organ pathological changes, inflammatory infiltration, inflammatory cytokine expression, and serum organ injury biomarkers was demonstrated. HS increased OMV production by intestinal microbiota; OMVs were absorbed by different organs in vivo, and were especially enriched in the liver and lung. Compared to control OMVs, infusion with HS OMVs induced significant organ pathological changes, elevated inflammatory cell (macrophages and neutrophil) infiltration, inflammatory cytokine (TNF-ɑ, IL-1β, IL-6) expression, as well as serum biomarkers of organ injury. Similarly, inhibition of endogenous OMVs alleviated these organ injury indicators induced by HS. To our knowledge, the present study is the first to illustrate that OMVs induce acute organ impairment during severe HS, offering a foundation for subsequent studies and providing novel therapeutic targets.
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http://dx.doi.org/10.1016/j.micpath.2022.105653DOI Listing
June 2022

Genome-wide identification of chicken bursae of Fabricius miRNAs in response to very virulent infectious bursal disease virus.

Arch Virol 2022 Jun 25. Epub 2022 Jun 25.

College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang District, Harbin, People's Republic of China.

Infectious bursal disease virus (IBDV) can cause a highly contagious immunosuppressive disease in young chickens. MicroRNAs (miRNAs) are crucial regulators of gene expression and are involved in the pathogenesis of IBDV infection. To investigate the roles of miRNA in chicken bursae of Fabricius in response to very virulent IBDV (vvIBDV) infection, RNA sequencing was performed to compare the small RNA libraries from uninfected and vvIBDV-infected group which was infected for 3 days. A total of 77 differentially expressed (DE) miRNAs were identified in BF, of which 42 DE miRNAs were upregulated and 35 DE miRNAs were downregulated. A gene ontology analysis showed that genes associated with cellular processes, cells, and binding were enriched. Moreover, pathway analyses suggested that apoptosis, T cell receptor signaling pathways, and chemokine signaling pathways may be activated following vvIBDV infection. In addition, we predicted the target genes of DE miRNAs and constructed an miRNA-mRNA regulatory network. In total, 189 pairs of miRNA-target genes were identified, comprising 67 DE miRNAs and 73 mRNAs. In this network, gga-miR-1684b-3p was identified with the highest fold change, as well as gga-miR-1788-3p and gga-miR-3530-5p showed a high degree of change. The above three miRNAs were considered to play vital roles in vvIBDV-host interactions. This study was the first to perform a comprehensive analysis of DE miRNAs in the bursa of Fabricius in response to vvIBDV infection, and it provided new insights into molecular mechanisms underlying vvIBDV infection and pathogenesis.
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http://dx.doi.org/10.1007/s00705-022-05496-6DOI Listing
June 2022

Spatial Inequalities and Influencing Factors of Self-Rated Health and Perceived Environmental Hazards in a Metropolis: A Case Study of Zhengzhou City, China.

Int J Environ Res Public Health 2022 Jun 20;19(12). Epub 2022 Jun 20.

Key Research Institute of Yellow River Civilization and Sustainable Development & Collaborative Innovation, Center on Yellow River Civilization Jointly Built by Henan Province and Ministry of Education, Henan University, Kaifeng 475001, China.

Research on environmental pollution and public health has aroused increasing concern from international scholars; particularly, environmental hazards are among the important issues in China, focusing public attention on significant health risks. However, there are few studies concentrated on how perceived environmental hazards are characterized by spatial variation and on the impact of these risks on residents' health. Based on a large-scale survey of Zhengzhou City in 2020, we investigated how the self-rated health of residents and the environmental hazards perceived by them were spatially inequal at a fine (subdistrict) scale in Zhengzhou City, China, and examined the relationship among self-rated health, environmental hazards, and geographical context. The Getis-Ord Gi* method was applied to explore the spatially dependent contextual (neighborhood) effect on environmental health inequality, and the ordered multivariate logistic regression method was used to examine the correlative factors with environmental hazards, geographical context, and health inequality. The results reveal that self-rated health and environmental hazards were disproportionately distributed across the whole city and that these distributions showed certain spatial cluster characteristics. The hot spot clusters of self-rated health had favorable environmental quality where the hot spot clusters of environmental hazards were located and vice versa. In addition, health inequality was evident and was related to gender, income level, educational attainment, and housing area of residents, and the inequalities of environmental hazards existed with respect to income and housing area. Meanwhile, environmental risk inequalities associated with the social vulnerability of residents (the poor and those with low educational attainment) were obvious, with those residents experiencing a disproportionately high exposure to environmental hazards and reporting bad health conditions. The role of the geographical context (subdistrict location feature) also helps to explain the spatial distribution of health and environmental inequalities. Residents with better exposure to green coverage generally reported higher levels of self-rated health condition. In addition, the geographical location of the subdistrict also had a significant impact on the difference in residents' self-rated health status. The purpose of this study is to provide reference for policy makers to optimize the spatial pattern of urban public services and improve public health and environmental quality at a fine scale.
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http://dx.doi.org/10.3390/ijerph19127551DOI Listing
June 2022

Prediction of the Old-Age Dependency Ratio in Chinese Cities Using DMSP/OLS Nighttime Light Data.

Int J Environ Res Public Health 2022 Jun 11;19(12). Epub 2022 Jun 11.

Institute of Ageing Studies, Peking University, Beijing 100871, China.

The old-age dependency ratio (ODR) is an important indicator reflecting the degree of a regional population's aging. In the context of aging, this study provides a timely and effective method for predicting the ODR in Chinese cities. Using the provincial ODR from the Seventh National Population Census and Defense Meteorological Satellite Program/Operational Linescan System (DMSP/OLS) nighttime light data, this study aims to predict and analyze the spatial correlation of the municipal ODR in Chinese cities. First, the prediction model of the ODR was established with curve regression. Second, the spatial structure of the municipal ODR was investigated using the Moran's method. The experimental results show the following: (1) the correlation between the sum of the nighttime light and ODR is greater than the mean of nighttime light in the study areas; (2) the Sigmoid model fits better than other regression models using the provincial ODR in the past ten years; and (3) there exists an obvious spatial agglomeration and dependence on the municipal ODR. The findings indicate that it is reasonable to use nighttime light data to predict the municipal ODR in large and medium-sized cities. Our approach can provide support for future regional censuses and spatial simulations.
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http://dx.doi.org/10.3390/ijerph19127179DOI Listing
June 2022

A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton ( L.).

Plants (Basel) 2022 Jun 15;11(12). Epub 2022 Jun 15.

College of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, China.

As a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, virus-induced gene silencing (VIGS) technology was used to analyze the biological function of in cotton drought stress tolerance. Meanwhile, we used yeast two-hybrid and bimolecular fluorescence complementation assays to examine the interaction between GhROP3 and GhGGB. has a high expression level in cotton true leaves and roots, and responds to drought, high salt, cold, heat stress, and exogenous abscisic acid (ABA) and auxin (IAA) treatments. Silencing improved the drought tolerance of cotton. The water loss rates (WLR) of detached leaves significantly reduced in silenced plants. Also, the relative water content (RWC) and total contents of chlorophyll (Chl) and proline (Pro) of leaves after drought stress and the activities of three antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) significantly increased, whereas the contents of hydrogen peroxide (HO) and malondialdehyde (MDA) significantly reduced. In the leaves of silenced plants, the expression of genes related to ABA synthesis and its related pathway was significantly upregulated, and the expression of decomposition-related gene and genes related to IAA synthesis and its related pathways was significantly downregulated. It indicated that was a negative regulator of cotton response to drought by participating in the negative regulation of the ABA signaling pathway and the positive regulation of the IAA signaling pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhROP3 protein interacted with the GhGGB protein in vivo and in vitro. This study provided a theoretical basis for the in-depth investigation of the drought resistance-related molecular mechanism of the gene and the biological function of the gene.
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http://dx.doi.org/10.3390/plants11121580DOI Listing
June 2022

Radiomic Signatures for Predicting Receptor Status in Breast Cancer Brain Metastases.

Front Oncol 2022 6;12:878388. Epub 2022 Jun 6.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Backgrounds: A significant proportion of breast cancer patients showed receptor discordance between primary cancers and breast cancer brain metastases (BCBM), which significantly affected therapeutic decision-making. But it was not always feasible to obtain BCBM tissues. The aim of the present study was to analyze the receptor status of primary breast cancer and matched brain metastases and establish radiomic signatures to predict the receptor status of BCBM.

Methods: The receptor status of 80 matched primary breast cancers and resected brain metastases were retrospectively analyzed. Radiomic features were extracted using preoperative brain MRI (contrast-enhanced T1-weighted imaging, T2-weighted imaging, T2 fluid-attenuated inversion recovery, and combinations of these sequences) collected from 68 patients (45 and 23 for training and test sets, respectively) with BCBM excision. Using least absolute shrinkage selection operator and logistic regression model, the machine learning-based radiomic signatures were constructed to predict the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) status of BCBM.

Results: Discordance between the primary cancer and BCBM was found in 51.3% of patients, with 27.5%, 27.5%, and 5.0% discordance for ER, PR, and HER2, respectively. Loss of receptor expression was more common (33.8%) than gain (18.8%). The radiomic signatures built using combination sequences had the best performance in the training and test sets. The combination model yielded AUCs of 0.89, 0.88, and 0.87, classification sensitivities of 71.4%, 90%, and 87.5%, specificities of 81.2%, 76.9%, and 71.4%, and accuracies of 78.3%, 82.6%, and 82.6% for ER, PR, and HER2, respectively, in the test set.

Conclusions: Receptor conversion in BCBM was common, and radiomic signatures show potential for noninvasively predicting BCBM receptor status.
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http://dx.doi.org/10.3389/fonc.2022.878388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207517PMC
June 2022

Geometry-independent antenna based on Epsilon-near-zero medium.

Nat Commun 2022 Jun 22;13(1):3568. Epub 2022 Jun 22.

Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.

It is well known that electromagnetic radiation from radiating elements (e.g., antennas, apertures, etc.) shows dependence on the element's geometry shape in terms of operating frequencies. This basic principle is ubiquitous in the design of radiators in multiple applications spanning from microwave, to optics and plasmonics. The emergence of epsilon-near-zero media exceptionally allows for an infinite wavelength of electromagnetic waves, manifesting exotic spatially-static wave dynamics which is not dependent on geometry. In this work, we analyze theoretically and verify experimentally such geometry-independent features for radiation, thus presenting a novel class of radiating resonators, i.e., antennas, with an operating frequency irrelevant to the geometry shape while only determined by the host material's dispersions. Despite being translated into different shapes and topologies, the designed epsilon-near-zero antenna resonates at a same frequency, while exhibiting very different far-field radiation patterns, with beams varying from wide to narrow, or even from single to multiple. Additionally, the photonic doping technique is employed to facilitate the high-efficiency radiation. The material-determined geometry-independent radiation may lead to numerous applications in flexible design and manufacturing for wireless communications, sensing, and wavefront engineering.
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http://dx.doi.org/10.1038/s41467-022-31013-zDOI Listing
June 2022

Toxicity evaluation of polycyclic aromatic hydrocarbons (PAHs) in soils of coal chemical industry areas, North China.

Environ Geochem Health 2022 Jun 22. Epub 2022 Jun 22.

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

Objectives of this study were to investigate the concentrations, distributions, toxicities, and risk assessment of 16 polycyclic aromatic hydrocarbons in surface soils surrounding a coal chemical industrial zone in the southeast of Shanxi province, China. A total of 52 topsoil samples were collected from different land-use areas: cereal agriculture, roadsides, and parkland. Results show that the total PAHs (∑PAHs) ranged from 3.87 × 10 to 116 × 10 µg kg and that the total carcinogenicity PAHs (∑BPAHs) ranged from 3.11 × 10 to 94.2 × 10 µg kg, with the highest concentration of ∑PAHs noted in the RS samples, followed by PS and AS. The entire risk quotient of all PAH maximum permissible concentrations (RQ∑PAH) was greater than 1.0, and the minimum concentration entire risk quotient (RQ∑PAH) of 84.3% of all samples was higher than 800. The value of the total toxicity equivalent concentration of PAH (PAHBapeq) for areas surrounding the coal chemical industrial zone was higher than the value of the standard level, and the incremental lifetime cancer risk (ILCR) far exceeds the U.S. EPA's risk standard. The toxic properties of PAHs indicated that the soils in the survey areas have a high risk to human health and the environment.
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http://dx.doi.org/10.1007/s10653-022-01306-9DOI Listing
June 2022

Inhibition of visfatin alleviates sepsis-induced intestinal damage by inhibiting Hippo signaling pathway.

Inflamm Res 2022 Jun 22. Epub 2022 Jun 22.

Department of Anesthesiology, Peking University Third Hospital, No. 49, North Garden Street, Haidian District, Beijing, 100191, China.

Background: The aim of this study is to investigate role of Visfatin, one of the pro-inflammatory adipokines, in sepsis-induced intestinal injury and to clarify the potential mechanism.

Methods: C57BL/6 mice underwent cecal ligation and puncture (CLP) surgery to establish sepsis model in vivo. Intestinal epithelial cells were stimulated with LPS to mimic sepsis-induced intestinal injury in vitro. FK866 (the inhibitor of Visfatin) with or without XMU-MP-1 (the inhibitor of Hippo signaling) was applied for treatment. The expression levels of Visfatin, NF-κB and Hippo signaling pathways-related proteins were detected by western blot or immunohistochemistry. The intestinal cell apoptosis and intestinal injury were investigated by TUNEL staining and H&E staining, respectively. ELISA was used to determine the production of inflammatory cytokines.

Results: The expression of Visfatin increased in CLP mice. FK866 reduced intestinal pathological injury, inflammatory cytokines production, and intestinal cell apoptosis in sepsis mice. Meanwhile, FK866 affected NF-κB and Hippo signaling pathways. Additionally, the effects of FK866 on inflammatory response, apoptosis, Hippo signaling and NF-κB signaling were partly abolished by XMU-MP-1, the inhibitor of Hippo signaling. In vitro experiments also revealed that FK866 exhibited a protective role against LPS-induced inflammatory response and apoptosis in intestinal cells, as well as regulating NF-κB and Hippo signaling, whereas addition of XMU-MP-1 weakened the protective effects of FK866.

Conclusion: In short, this study demonstrated that inhibition of Visfatin might alleviate sepsis-induced intestinal injury through Hippo signaling pathway, supporting a further research on Visfatin as a therapeutic target.
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http://dx.doi.org/10.1007/s00011-022-01593-zDOI Listing
June 2022

Pancreatic involvement in Erdheim-Chester disease: a case report and review of the literature.

BMC Gastroenterol 2022 Jun 21;22(1):302. Epub 2022 Jun 21.

Department of Hematology, State Key Laboratory of Complex, Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Erdheim-Chester disease (ECD) is a rare form of non-Langerhans cell histiocytosis characterized by infiltration of lipid-laden foamy macrophages within different tissues. Clinical manifestations of ECD are highly heterogeneous. Bone lesions are found in 80%-95% of patients, while extraosseous lesions usually involve the cardiovascular system, retroperitoneum, central nervous system (CNS), and skin. Pancreatic involvement in ECD has barely been reported.

Case Presentation: A 29-year-old female initially presented with menoxenia, diabetes insipidus and diabetes mellitus. 18F-fluorodeoxyglucose positron emission tomography-computed tomography (18F-FDG-PET/CT) revealed hypermetabolic foci in the bilateral frontal lobe, saddle area, and pancreas. A 99mTc-MDP bone scrintigraphy scan revealed symmetrical increased uptake in distal femoral and proximal tibial metaphysis, which was confirmed to be osteosclerosis by high-resolution peripheral quantitative computed tomography. The patient underwent incomplete resection of the sellar mass. Histological examination of biopsies showed histiocytic aggregates, which were positive for S100 and negative for CD1a and CD207 on immunohistochemistry. Enhanced abdominal CT scan showed hypointense nodules within the body and tail of the pancreas. Endoscopic ultrasonography guided fine-needle aspiration (EUS-FNA) found no evidence of malignancy. She was diagnosed with ECD and treated with high-dose IFN-α. Repeated examinations at three-and eight-months post treatment revealed markedly reduction of both intracranial and pancreatic lesions.

Conclusions: ECD is a rare histiocytic neoplasm that can involve almost every organ, whereas pancreatic involvement has barely been reported to date. Here, we present the rare case of pancreatic lesions in ECD that responded well to interferon-α. We further reviewed reports of pancreatic involvement in histiocytic disorders and concluded the characteristics of such lesions to help diagnosis and treatment, in which these lesions mimicked pancreatic adenocarcinoma and caused unnecessary invasive surgeries.
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http://dx.doi.org/10.1186/s12876-022-02378-8DOI Listing
June 2022

Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B.

Mol Cell 2022 Jun 12. Epub 2022 Jun 12.

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China; Peking University Institute of Advanced Agricultural Sciences, Weifang 261325, China. Electronic address:

Light and temperature in plants are perceived by a common receptor, phytochrome B (phyB). How phyB distinguishes these signals remains elusive. Here, we report that phyB spontaneously undergoes phase separation to assemble liquid-like droplets. This capacity is driven by its C terminus through self-association, whereas the intrinsically disordered N-terminal extension (NTE) functions as a biophysical modulator of phase separation. Light exposure triggers a conformational change to subsequently alter phyB condensate assembly, while temperature sensation is directly mediated by the NTE to modulate the phase behavior of phyB droplets. Multiple signaling components are selectively incorporated into phyB droplets to form concentrated microreactors, allowing switch-like control of phyB signaling activity through phase transitions. Therefore, light and temperature cues are separately read out by phyB via allosteric changes and spontaneous phase separation, respectively. We provide a conceptual framework showing how the distinct but highly correlated physical signals are interpreted and sorted by one receptor.
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http://dx.doi.org/10.1016/j.molcel.2022.05.026DOI Listing
June 2022

High-fidelity simulation duration and learning outcomes among undergraduate nursing students: A systematic review and meta-analysis.

Nurse Educ Today 2022 Jun 11;116:105435. Epub 2022 Jun 11.

Kiang Wu Nursing College of Macau SAR, China; Kiang Wu Nursing College of Macau, Est. Repouso No. 35, R/C, Macau SAR, China. Electronic address:

Objectives: To explore the effects of different lengths of the three key steps (prebriefing, simulation, and debriefing) of high-fidelity simulation (HFS) on the knowledge and skills of undergraduate nursing students.

Design: A systematic review and meta-analysis.

Data Sources: A systematic search was conducted for Chinese and English publications from the Web of Science, PubMed, Embase, Cochrane, and two Chinese databases (Wanfang and CNKI) up to November 24, 2021.

Review Methods: Two independent raters screened the retrieved studies and extracted data based on a coding protocol from the studies that met the inclusion criteria. Data were synthesized using meta-analytic procedures based on a random-effect model and computing effect sizes by standard mean differences (SMD) with a 95% confidence interval (CI).

Results: Forty-four studies were included, and 23 studies were analyzed. High-fidelity simulation (HFS) with debriefing of 10 min or less (SMD = 1.18), simulation of 15-20 min (SMD = 1.43), and debriefing of 11-30 min (SMD = 1.19) showed larger effect sizes for knowledge, while HFS with debriefing of >10 min (SMD = 0.91), simulation of 15 min or less (SMD = 0.89), and over 30 min of debriefing cultivation (SMD = 0.84) showed larger effect sizes for skill.

Conclusions: During the prebriefing, simulation, and debriefing, shorter sessions are more effective for improving knowledge in BSN students, whereas longer sessions are most effective for improving skills in BSN students. Nurse educators can schedule HFS to meet the expectations of learning outcomes based on the actual situation.
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http://dx.doi.org/10.1016/j.nedt.2022.105435DOI Listing
June 2022

Transcriptome analysis reveals the spinal expression profiles of non-coding RNAs involved in anorectal malformations in rat fetuses.

J Pediatr Surg 2022 Jun 2. Epub 2022 Jun 2.

Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:

Background: Despite improvements in anorectal malformation (ARM) therapy, patients might still experience post-operative problems such as fecal incontinence, constipation, and soiling. In particular, the dysplasia of the lumbosacral spinal cord in ARM patients is a major disorder that affects fecal function post-operation. However, the pathological mechanisms involved are still unclear.

Methods: The non-coding RNAs (ncRNAs) in the lumbosacral spinal cord of fetal rats with ethylenethiourea-induced ARM were identified using RNA sequencing (RNA-seq) and examined to determine their potential function. The lumbosacral spinal cord was isolated on embryonic day 17 for subsequent RNA extraction and RNA-seq. The transcriptome data was analyzed using bioinformatics analysis, followed by validation using quantitative reverse transcription PCR.

Results: Compared to the control group, 26 differentially expressed microRNAs (DEMs; 22 upregulated, 4 downregulated) and 112 differentially expressed long non-coding RNAs (63 upregulated, 49 downregulated) were identified in the ARM group. Several DEMs related to development, namely miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-200a-5p, and miR-429, were selected for further analysis. Notably, compared to the control, the relative expression of miR-200 family members was highly upregulated in ARM fetal rats. Furthermore, GO and KEGG enrichment and miRNA-transcription factor-lncRNA/mRNA network analysis was explored to show molecular mechanism underlying DEMs.

Conclusions: Our findings suggest the involvement of ncRNAs, especially the miR-200 family members, in the pathogenesis of lumbosacral spinal cord dysplasia in ARM fetal rats.
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http://dx.doi.org/10.1016/j.jpedsurg.2022.05.019DOI Listing
June 2022

Effect of Steam Explosion on Structural Characteristics of β-Conglycinin and Morphology, Chemical Compositions of Soybean Meal.

Front Nutr 2022 2;9:896664. Epub 2022 Jun 2.

College of Agronomy, Liaocheng University, Liaocheng, China.

In this study, steam explosion was applied as a means to degrade β-conglycinin. We investigated changes in morphology, the chemical composition of soybean meal, and the structural characteristics of β-conglycinin. The results showed that steam explosion at 0.7 MPa for 8 min could effectively decrease the β-conglycinin content of soybean meal while the histamine content was not increased. The structural characteristics of soybean meal proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), and X-ray diffraction (XRD). Steam explosion caused the degradation of high weight proteins and reduced the band density of α', α, and β subunits in β-conglycinin. The micro-surface of soybean meal seemed to be in the cracked or puffed stage and the color became brown or dark after steam explosion. Steam explosion facilitated the dissolution of water-extractable arabinoxylans, which are 4.81 fold higher than that of native soybean meal. Phytic acid was exposed to the hydrothermal environment of the steam explosion process and consequently degraded by 12.95-24.69%. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of soybean meal extract was gradually increased from 20.70 to 33.71% with the rising of treated pressure from 0.3 to 0.7 MPa, which was 1.11-1.81 fold of native extract. The steam explosion may be a new modification technology that could decrease antigenicity, and steam-exploded soybean meal (0.7 MPa, 8 min) with lower β-conglycinin and phytic acid content that could be widely used in food products.
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http://dx.doi.org/10.3389/fnut.2022.896664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202520PMC
June 2022

Hepatoprotective Effects of Glycyrrhetinic Acid on Lithocholic Acid-Induced Cholestatic Liver Injury Through Choleretic and Anti-Inflammatory Mechanisms.

Front Pharmacol 2022 31;13:881231. Epub 2022 May 31.

Laboratory of Clinical Pharmacokinetics, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Cholestasis is a clinical syndrome triggered by the accumulation and aggregation of bile acids by subsequent inflammatory responses. The present study investigated the protective effect of glycyrrhetinic acid (GA) on the cholestatic liver injury induced by lithocholic acid (LCA) from both anti-inflammatory and choleretic mechanistic standpoints. Male C57BL/6 mice were treated with LCA twice daily for 4 days to induce intrahepatic cholestasis. GA (50 mg/kg) and pregnenolone 16α-carbonitrile (PCN, 45 mg/kg) were intraperitoneally injected 3 days before and throughout the administration of LCA, respectively. Plasma biochemical indexes were determined by assay kits, and hepatic bile acids were quantified by LC-MS/MS. Hematoxylin and eosin staining of liver sections was performed for pathological examination. Protein expression of the TLRs/NF-κB pathway and the mRNA levels of inflammatory cytokines and chemokines were examined by Western blotting and PCR, respectively. Finally, the hepatic expression of pregnane X receptor (PXR) and farnesoid X receptor (FXR) and their target genes encoding metabolic enzymes and transporters was evaluated. GA significantly reversed liver necrosis and decreased plasma ALT and ALP activity. Plasma total bile acids, total bilirubin, and hepatic bile acids were also remarkably preserved. More importantly, the recruitment of inflammatory cells to hepatic sinusoids was alleviated. Additionally, the protein expression of TLR2, TLR4, and p-NF-κBp65 and the mRNA expression of CCL2, CXCL2, IL-1β, IL-6, and TNF-α were significantly decreased. Moreover, GA significantly increased the expression of hepatic FXR and its target genes, including BSEP, MRP3, and MRP4. In conclusion, GA protects against LCA-induced cholestatic liver injury by inhibiting the TLR2/NF-κB pathway and upregulating hepatic FXR expression.
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http://dx.doi.org/10.3389/fphar.2022.881231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194553PMC
May 2022

Recent advances in acoustic microfluidics and its exemplary applications.

Biomicrofluidics 2022 May 13;16(3):031502. Epub 2022 Jun 13.

School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China.

Acoustic-based microfluidics has been widely used in recent years for fundamental research due to its simple device design, biocompatibility, and contactless operation. In this article, the basic theory, typical devices, and technical applications of acoustic microfluidics technology are summarized. First, the theory of acoustic microfluidics is introduced from the classification of acoustic waves, acoustic radiation force, and streaming flow. Then, various applications of acoustic microfluidics including sorting, mixing, atomization, trapping, patterning, and acoustothermal heating are reviewed. Finally, the development trends of acoustic microfluidics in the future were summarized and looked forward to.
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http://dx.doi.org/10.1063/5.0089051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197543PMC
May 2022

EpiTopics: A dynamic machine learning model to predict and inform non-pharmacological public health interventions from global news reports.

STAR Protoc 2022 06 13;3(2):101463. Epub 2022 Jun 13.

School of Computer Science, McGill University, Montreal, QC H3A 0G4, Canada.

Non-pharmacological interventions (NPIs) are important for controlling infectious diseases such as COVID-19, but their implementation is currently monitored in an ad hoc manner. To address this issue, we present a three-stage machine learning framework called EpiTopics to facilitate the surveillance of NPI. In this protocol, we outline the use of transfer-learning to address the limited number of NPI-labeled documents and topic modeling to support interpretation of the results. For complete details on the use and execution of this protocol, please refer to Wen et al. (2022).
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http://dx.doi.org/10.1016/j.xpro.2022.101463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189439PMC
June 2022

Impact of mandatory COVID-19 shelter-in-place order on controlled substance use among rural versus urban communities in the United States.

J Rural Health 2022 Jun 16. Epub 2022 Jun 16.

Department of Psychiatry, University of Rochester Medical Center, Rochester, New York, USA.

Purpose: Mandatory COVID-19 shelter-in-place (SIP) orders have been imposed to fight the pandemic. They may also have led to unintended consequences of increased use of controlled substances especially among rural communities due to increased social isolation. Using the data from the American Association of Poison Control Centers, this study tests the hypothesis that the poison control centers received higher rates of calls related to exposures to controlled substances from rural counties than they did from urban counties during the SIP period.

Methods: Call counts received by the poison control centers between October 19, 2019 and July 6, 2020 due to exposure to controlled substance (methamphetamine, opioids, cocaine, benzodiazepines, and other narcotics) were aggregated to per-county-per-month-per-10,000 population exposure rates. A falsification test was conducted to reduce the possibility of spurious correlations.

Findings: During the study period, 2,649 counties in the United States had mandatory SIP orders. The rate of calls reporting exposure to any of the aforementioned controlled substances among the rural counties was higher (14%; P = .047) relative to the urban counties. This overall increase was due to increases in the rates of calls reporting exposure to opioids (26%; P = .017) and methamphetamine (39%; P = .077). Moreover, the rate of calls reporting exposures at home was also higher among the rural counties (14%; P = .069).

Conclusion: The mandatory SIP orders may have had an unintended consequence of exacerbating the use of controlled substances at home in rural communities relative to urban communities.
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http://dx.doi.org/10.1111/jrh.12688DOI Listing
June 2022

The effect of health literacy on COVID-19 vaccine hesitancy among community population in China: The moderating role of stress.

Vaccine 2022 Jun 8. Epub 2022 Jun 8.

Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, China; Division of Medical Psychology and Behavior Science, School of Medicine, Jinan University, Guangzhou, China; Jinan University-BioKangtai Vaccine Institute, Jinan University, Guangzhou City, Guangdong Province, China. Electronic address:

Background: The public's hesitant attitude is a major subjective barrier in promoting vaccination against COVID-19 to build herd immunity. The current study aimed to address how individual factors such as health literacy and perceived stress affect people's vaccine hesitancy of COVID-19 vaccine, and to provide insights for tailoring vaccine-promotion strategies.

Methods: With structured questionnaires, an online survey was conducted to address the relationship between the health literacy, perceived stress, and COVID-19 vaccine hesitancy among community population in mainland, China. Moderated analysis was conducted to test the effect of health literacy on vaccine hesitancy among people with different levels of perceived stress.

Results: 560 responses were collected in total. 39.8% of the participants reported vaccine hesitancy, and this rate was higher among younger people and female. Moreover, people with higher level of health literacy showed reduced vaccine hesitancy, while this effect was only significant among those with low or moderate level of stress. For people with high level of stress, no significant effect of health literacy was found.

Conclusions: The findings suggest that increasing people's health literacy could lead to reduced vaccine hesitancy in community sample. However, this effect disappeared when the stress level was high, suggesting other promotion services may need to be developed to increase the acceptance of COVID-19 vaccine. In conclusion, vaccine promotion strategies should be tailored for different populations, with taking account of individual's health literacy and perceived stress.
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http://dx.doi.org/10.1016/j.vaccine.2022.06.015DOI Listing
June 2022

Construction and investigation of circRNA-associated ceRNA regulatory network in molecular subtypes of breast cancer.

Curr Comput Aided Drug Des 2022 Jun 15. Epub 2022 Jun 15.

Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.

Background: Circular RNAs (circRNAs) act as competing endogenous RNAs (ceRNAs) that indirectly regulate gene expression and function by binding microRNAs (miRNAs). A growing body of evidence indicates that the ceRNA networks can be used as an effective method to investigate cancer; however, the construction and analysis of ceRNA networks, especially circRNA-miRNA-mRNA regulatory network, in different subtypes of breast cancer has not been previously performed.

Objectives: In the present study, we generated a ceRNA network to explore their roles in two BC subtypes, namely Luminal A and triple negative breast cancer (TNBC).

Methods: First, the expression profiles of circRNA, miRNA, and mRNA were downloaded from the GEO database, differentially expressed genes were obtained using GEO2R, and a ceRNA network, was constructed based on circRNA-miRNA pairs and miRNA-mRNA pairs, which was consisted of 10 circRNAs, 25 miRNAs and 39 mRNAs. Further studies of BC subtypes based on TCGA datasets were also performed to validate the effect of novel ceRNA network.

Results And Discussion: Then, the related genes in the regulatory network were analyzed by GO functional annotation and KEGG pathway enrichment. The analysis showed that targeted genes were enriched in 97 GO terms and 25 KEGG pathways, respectively, involved in the molecular typing of breast cancer. Meanwhile, Kaplan-Meier analysis revealed that three key genes (MKI67, DEF8, and GFRA1) were significantly associated with BC tumor differentiation and prognosis.

Conclusion: The current study provides a potential application of ceRNA network within BC subtypes, and may offer new targets for their diagnosis, therapy and prognosis.
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http://dx.doi.org/10.2174/1573409918666220615151614DOI Listing
June 2022

Erratum: Comparative study of nanostructured carriers of calcium phosphate and magnesium phosphate loaded with SRT1720 for the protection of HO-induced senescent endothelium.

Am J Transl Res 2022 15;14(5):3572-3573. Epub 2022 May 15.

Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Meilong Road 130, Shanghai 200237, China.

[This corrects the article on p. 2068 in vol. 10, PMID: 30093944.].
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185045PMC
May 2022

The Increased Tibiofemoral Rotation: A Potential Contributing Factor for Patellar Maltracking in Patients with Recurrent Patellar Dislocation.

Orthop Surg 2022 Jun 13. Epub 2022 Jun 13.

Sports Medicine Service, Beijing jishuitan hospital, Beijing, China.

Objective: The purpose of this study was to analyze the relationship between tibiofemoral rotation and patellar maltracking in patients with recurrent patellar dislocation.

Methods: A total of 143 consecutive knees (118 patients) with clinically diagnosed recurrent patellar dislocation from January 2018 to December 2019 were retrospectively analyzed. Patellar tilt angle and bisect offset index were recorded on axial CT to assesses the severity of patellar maltracking. Tibiofemoral rotation angle is measured by comparing the angle between the posterior femoral and tibial condylar lines on three-dimensional CT. The Pearson correlation was calculated to investigate the association between tibiofemoral rotation angle and patellar maltracking. Patients were divided into the rotation group (≥15°) and control group (<15°) based on the value of tibiofemoral rotation and a further comparison was performed. To further clarify the complicated relationship among tibial tubercle-trochlear groove (TT-TG), tibial tubercle-posterior cruciate ligament distance (TT-PCL), tibiofemoral rotation, and patellar maltracking, patients were divided into four subgroups according to the value of TT-TG and TT-PCL.

Results: The mean preoperative tibiofemoral rotation angle was 12° ± 6° (range, 0°-31°). Pearson correlation between patellar maltracking parameters (bisect offset index, patellar tilt angle) and various bony deformities found that the tibiofemoral rotation angle was moderately correlated with bisect offset index (r = 0.451, p < 0.001) and patellar tilt angle (r = 0.462, p < 0.001). Further results demonstrated that bisect offset index (152.1 vs 121.2, p < 0.001) and patellar tilt angle (41.2° vs 33.5°, p < 0.001) were significantly higher in the rotation group than that in control group. For patients with a TT-TG distance of >20 mm, the increased TT-TG distance was mainly caused by tibiofemoral rotation angle in group C (TT-TG > 20 mm, TT-PCL < 24 mm) and predominantly induced by tibial tubercle lateralization in group D (TT-TG > 20 mm, TT-PCL > 24 mm). Bisect offset index and patellar tilt angle were significantly higher in the group C than group D.

Conclusion: The increased tibiofemoral rotation angle is associated with patellar maltracking in patients with recurrent patellar dislocation. Patients with increased tibiofemoral rotation angle usually have more severe patellar maltracking.
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http://dx.doi.org/10.1111/os.13358DOI Listing
June 2022

Research on Rural Landscape Spatial Information Recording and Protection Based on 3D Point Cloud Technology under the Background of Internet of Things.

Comput Intell Neurosci 2022 2;2022:3772108. Epub 2022 Jun 2.

Faculty of Art and Design, Shandong Women's University, Jinan, Shandong 250300, China.

The village with historical and cultural accumulation not only is the witness of the interaction between human activities and natural environment but also contains a lot of intangible cultural heritage. It is an important research object in the history of human social development and has great reference value for the construction of modern Humayun settlements. With the continuous change of modern rural landscape, the traditional engineering drawing cannot meet the needs of people for spatial information measurement. There are some problems such as slow drawing, low precision, and poor benefit, so computer drawing must be used. Therefore, this paper puts forward the research on rural landscape spatial information recording and protection based on 3D point cloud technology under the background of Internet of Things and realizes the recording of rural landscape spatial information through the construction of 3D point cloud spatial information recording model. The experimental results show that the improved unit point cloud spatial information recording model has better feature extraction effect and feature point extraction efficiency, and can classify the point cloud more carefully. At the same time, the scene 3D point cloud classification method based on conditional random field can better deal with the interference factors in the outdoor landscape and show a better classification effect.
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http://dx.doi.org/10.1155/2022/3772108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184149PMC
June 2022
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