Publications by authors named "Yue Jin"

330 Publications

N-Methyladenosine RNA Demethylase FTO Promotes Gastric Cancer Metastasis by Down-Regulating the m6A Methylation of ITGB1.

Front Oncol 2021 1;11:681280. Epub 2021 Jul 1.

Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, China.

Abnormal RNA m6A methylation is known to lead to the occurrence and progression of multiple cancers including gastric cancer (GC). However, the integrative effects of all m6A methylation regulators on GC prognosis are unclear. Our research aimed to globally analyze the prognosis values of all 33 m6A RNA methylation regulators in GC by univariate and multivariate Cox regression analyses. Among all 33 m6A RNA methylation regulators, fat mass and obesity-associated protein (FTO), an m6A demethylase, was identified as a key prognostic risk factor on overall survival (OS) of GC patients. It was found that FTO could promote GC cell migration and invasion abilities, and we predicted that ITGB1 was a demethylated target of FTO. Knockdown (KD) of FTO significantly down-regulated ITGB1 expression at both mRNA and protein levels and augmented ITGB1 mRNA m6A modification level. Moreover, overexpression (OE) of ITGB1 could partially reverse FTO-KD-inhibited migration and invasion of GC cells. Our study found that FTO was an independent risk factor for overall survival (OS) of GC patients and FTO could promote GC metastasis by upregulating the expression of Integrin β1(ITGB1) decreasing its m6A level. These results indicated that FTO can be a potent GC biomarker for prognosis prediction as well as a potential target in GC treatment.
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http://dx.doi.org/10.3389/fonc.2021.681280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282183PMC
July 2021

Rosmarinic acid relieves cisplatin-induced ovary toxicity in female mice via suppression of oxidative stress and inflammation.

J Biochem Mol Toxicol 2021 Jul 11:e22839. Epub 2021 Jul 11.

Department of Gynecology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Rosmarinic acid having potential anti-inflammatory and free radical scavenging activity. We examined the chemotherapeutic effect of rosmarinic against cisplatin (CIS)-induced ovarian toxicity via modulation of oxidative stress and inflammation. Swiss BALB mice used in experimental protocol and mice were divided into different groups. Intraperitoneal injection of CIS (7 mg/kg) was used for ovarian cancer induction. The rats were received rosmarinic acid (2.5, 5, and 10 mg/kg, body weight) treatment for 22 weeks. Body weight, ovary weight food, and water intake were estimated at regular time intervals. Hormonal and antioxidant parameters were estimated in the ovary tissue and serum at the end of the study. Cytokines, inflammatory, and apoptosis parameters were determined at the end of the study. Finally, the ovary tissue histopathology was performed at end of the experimental study. Rosmarinic acid significantly (p < 0.001) improved the body weight and reduced the ovary weight. Rosmarinic acid considerably reduced the hormonal assay parameters, such as antimullerian hormone, estradiol, luteinizing hormone, and follicle-stimulating hormone compared to model control mice. Rosmarinic treatment significantly (p < 0.001) reduced the level of nitric oxide, myeloperoxidase, and boosted the level of antioxidant parameters, such as glutathione, superoxide dismutase, catalase, and glutathione peroxidase in serum and ovary tissue. Rosmarinic acid downregulated the cytokines like interleukin-6, tumor necrosis factor-α, interleukin-1β; inflammatory parameter includes prostaglandin E , cyclooxygenase-2, and inducible nitric oxide synthase at a dose-dependently. Ovary tissue histopathology showed improvement after rosmarinic acid treatment. The result suggests that rosmarinic acid is a protective effect in ameliorating CIS-induced ovary toxicity via alteration of inflammatory and apoptosis parameters.
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http://dx.doi.org/10.1002/jbt.22839DOI Listing
July 2021

Identification of Winter Habit Bread Wheat Landraces in the National Small Grains Collection with Resistance to Emerging Stem Rust Pathogen Variants.

Plant Dis 2021 Jul 7. Epub 2021 Jul 7.

USDA-ARS Small Grains and Potato Germplasm Research Unit, 57758, Aberdeen, Idaho, United States;

Wheat stem rust caused by Puccinia graminis f. sp. tritici (Pgt) is a widespread and recurring threat to wheat production. Emerging Pgt variants are rapidly overcoming major gene resistance deployed in wheat cultivars and new sources of race-nonspecific resistance are urgently needed. The National Small Grains Collection (NSGC) contains thousands of wheat landrace accessions that may harbor unique and broadly effective sources of resistance to emerging Pgt variants. All NSGC available facultative and winter-habit bread wheat landraces were tested in a field nursery in St. Paul, MN against a bulk collection of six common U.S. Pgt races. Infection response and severity data were collected on 9,192 landrace accessions at the soft-dough stage and resistant accessions were derived from single spikes. Derived accessions were tested in St. Paul a second time to confirm resistance and in a field nursery in Njoro, Kenya against emerging races of Pgt with virulence to many known resistance genes including Sr24, Sr31, Sr38, and SrTmp. Accessions resistant in the St. Paul field were also tested at the seedling stage with up to 13 Pgt races, including TTKSK and TKTTF, and with 19 molecular markers linked with known stem rust resistance genes or genes associated with modern breeding practices. Forty-five accessions were resistant in both U.S. and Kenya field nurseries and lacked alleles linked with known stem rust resistance genes. Accessions with either moderate or strong resistance in the U.S. and Kenya field nurseries and with novel seedling resistance will be prioritized for further study.
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http://dx.doi.org/10.1094/PDIS-04-21-0743-REDOI Listing
July 2021

Obstructive sleep apnea after pharyngeal flap surgery for velopharyngeal insufficiency in cleft patients.

J Plast Reconstr Aesthet Surg 2021 Jun 20. Epub 2021 Jun 20.

Department of Stomatology, the Affiliated Hospital of Qingdao University, No. 16, Jiangsu Road, Qingdao 266003, China; School of Stomatology, Qingdao University, Qingdao, Shandong 266071, China. Electronic address:

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http://dx.doi.org/10.1016/j.bjps.2021.06.009DOI Listing
June 2021

Hierarchical and Stable Multiagent Reinforcement Learning for Cooperative Navigation Control.

IEEE Trans Neural Netw Learn Syst 2021 Jun 28;PP. Epub 2021 Jun 28.

We solve an important and challenging cooperative navigation control problem, Multiagent Navigation to Unassigned Multiple targets (MNUM) in unknown environments with minimal time and without collision. Conventional methods are based on multiagent path planning that requires building an environment map and expensive real-time path planning computations. In this article, we formulate MNUM as a stochastic game and devise a novel multiagent deep reinforcement learning (MADRL) algorithm to learn an end-to-end solution, which directly maps raw sensor data to control signals. Once learned, the policy can be deployed onto each agent, and thereby, the expensive online planning computations can be offloaded. However, to solve MNUM, traditional MADRL suffers from large policy solution space and nonstationary environment when agents make decisions independently and concurrently. Accordingly, we propose a hierarchical and stable MADRL algorithm. The hierarchical learning part introduces a two-layer policy model to reduce the solution space and uses an interlaced learning paradigm to learn two coupled policies. In the stable learning part, we propose to learn an extended action-value function that implicitly incorporates estimations of other agents' actions, based on which the environment's nonstationarity caused by other agents' changing policies can be alleviated. Extensive experiments demonstrate that our method can converge in a fast way and generate more efficient cooperative navigation policies than comparable methods.
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http://dx.doi.org/10.1109/TNNLS.2021.3089834DOI Listing
June 2021

Early rehabilitation nursing in ICU promotes rehabilitation of patients with respiratory failure treated with invasive mechanical ventilation.

Am J Transl Res 2021 15;13(5):5232-5239. Epub 2021 May 15.

Intensive Care Unit, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University Changzhou 213000, Jiangsu Province, China.

Objective: This paper aimed at exploring the application value of early rehabilitation nursing (ERN) in intensive care unit (ICU) for nursing patients with respiratory failure (RF) treated with invasive mechanical ventilation (IMV).

Methods: Admitted to the ICU of our hospital from January 2019 to June 2020, 172 RF patients that was treated with IMV were selected as the research objects. Those in the general group (n=80) received routine nursing in ICU, whereas those in the recovery group (n=92) received ERN on the basis of the general group. The recovery of their vital signs, blood gas functions and pulmonary functions was monitored. Their treatment time in ICU, mechanical ventilation time (MVT), total hospitalization time (THT) and incidence of complications were recorded. Their negative emotions, quality of life (QOL) and nursing satisfaction were assessed.

Results: After intervention, the body temperature, the respiratory rate and the heart rate in the recovery group were lower than those in the general group (P<0.05). The arterial partial pressure of oxygen (PaO) and blood oxygen saturation (SpO) were remarkably higher in the recovery group, while the arterial partial pressure of carbon dioxide (PaCO) was remarkably lower (P<0.05). One-second forced expiratory volume (FEV), FEV/forced vital capacity (FVC) and FEV% were remarkably higher in the recovery group (P<0.05). The treatment time in ICU, the MVT and the THT were remarkably shorter in the recovery group (P<0.05). During intervention, the total incidence of complications was lower in the recovery group (P<0.05). While after intervention, the scores of the Self-rating Anxiety Scale (SAS), the Self-rating Depression Scale (SDS) and the St. George's Respiratory Questionnaire (SGRQ; symptom, activity, impact) were lower in the recovery group, but the nursing satisfaction was remarkably higher (P<0.05).

Conclusion: During the treatment of RF patients with IMV, ERN can promote their recovery, reduce the incidence of complications, relieve their negative emotions, and improve their QOL and nursing satisfaction. So, this nursing model is worthy of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205794PMC
May 2021

A Combination of Geniposide and Chlorogenic Acid Combination Ameliorates Nonalcoholic Steatohepatitis in Mice by Inhibiting Kupffer Cell Activation.

Biomed Res Int 2021 13;2021:6615881. Epub 2021 May 13.

Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

The incidence of nonalcoholic steatohepatitis (NASH) is increasing worldwide. Activation of Kupffer cells (KCs) is central to the development of diet-induced NASH. We investigated whether a combination of two active chemical components, geniposide and chlorogenic acid (GC), at a specific ratio (67 : 1), ameliorates diet-induced NASH and the underlying mechanisms involved. C57BL/6J mice exposed to a high-fat and high-cholesterol (HFHC) diet containing cholesterol, choline, and high-sugar drinking water, as well as RAW264.7 cells stimulated with lipopolysaccharide (LPS) were studied. The combination exerted a therapeutic effect on HFHC-induced NASH in mice. Simultaneously, GC was found to reduce the expression of cytokines secreted by hepatic macrophages, including tumor necrosis factor- (TNF-), interleukin-1 (IL-1), IL-1, IL-6, monocyte chemotactic protein 1 (MCP-1), and granulocyte-macrophage colony-stimulating factor (GM-CSF). Moreover, GC reduced the number of KCs expressing F4/80. Furthermore, TNF-, inducible nitric oxide synthase (INOS), IL-1, and IL-6 mRNA and TNF- protein expression levels were suppressed upon GC treatment in RAW264.7 cells. Our findings suggest that GC has a strong anti-inflammatory effect in NASH, and this effect can be attributed to the suppression of KC activity in the liver.
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http://dx.doi.org/10.1155/2021/6615881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140849PMC
May 2021

Dietary Inflammatory Index and Ovarian Cancer Risk: A Meta-Analysis.

Nutr Cancer 2021 Jun 1:1-10. Epub 2021 Jun 1.

Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Existing evidence suggests diet and chronic inflammation as risk factors in ovarian cancer (OC) development. We aim to conduct a meta-analysis exploring possible associations between dietary inflammatory potential and OC. A systematic search was conducted through PubMed, Scopus, Embase, and PMC databases for studies reporting relationships between dietary inflammatory potential and OC risk published up to September 2020. We included six studies for stepwise analysis, of which 5,468 among 197,086 individuals developed OC. Pooled odds ratios (ORs) were calculated by fixed-effects models, while heterogeneity was assessed by Q test and I statistic. The results revealed a positive association between dietary inflammatory potential measured by the Dietary Inflammatory Index (DII) and OC( < 0.05). Individuals with higher DII scores had a 42% increased risk of OC incidence [OR = 1.42, 95% confidence interval (CI): 1.19-1.65]. The analysis considering DII as a continuous variable showed an increased risk of 10% for 1-point increase of DII(OR = 1.10, 95% CI: 1.06-1.14). Subgroup analysis revealed that increased risk of OC in individuals with higher DII scores vs. those with lower DII was only significant among post-menopausal women(OR = 1.72, 95% CI: 1.26-2.21) rather than those pre/peri-menopausal(OR = 1.21, 95% CI: 0.63-1.79). Pro-inflammatory diets with higher DII score were significantly related to increased OC risk among post-menopausal women.
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http://dx.doi.org/10.1080/01635581.2021.1931366DOI Listing
June 2021

Artificial Intelligence Based on Blood Biomarkers Including CTCs Predicts Outcomes in Epithelial Ovarian Cancer: A Prospective Study.

Onco Targets Ther 2021 18;14:3267-3280. Epub 2021 May 18.

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Objective: We aimed to develop an ovarian cancer-specific predictive framework for clinical use platinum-sensitivity and prognosis using machine learning methods based on multiple biomarkers, including circulating tumor cells (CTCs).

Patients And Methods: We enrolled 156 epithelial ovarian cancer (EOC) patients, randomly assigned into the training and validation cohorts. Eight machine learning classifiers, including Random Forest (RF), Support Vector Machine, Gradient Boosting Machine, Conditional RF, Neural Network, Naive Bayes, Elastic Net, and Logistic Regression, were used to derive predictive information from 11 peripheral blood parameters, including CTCs. Through the advanced CanPatrol CTC-enrichment technique, we detect CTCs and classify them into subpopulations: epithelial, mesenchymal, and hybrids. Survival curves were generated by Kaplan-Meier method and calculated through the Log rank test.

Results: Machine learning techniques, especially the Random Forest classifier, were superior to conventional regression-based analyses in predicting multiple clinical parameters related to EOC. The values for the receiver operating characteristic (ROC) curve for segregating EOC with advanced clinical stages and platinum-sensitivity were 0.796 (95% CI, 0.727-0.866) and 0.809 (95% CI, 0.742-0.876), respectively. Stepwise, we used the unsupervised clustering analysis to identify EOC subgroups with significantly worse overall survival (OS), especially in the advanced-stage group with the p-value of 0.0018 (HR, 2.716; 95% CI, 1.602-4.605) for progression-free survival (PFS) and 0.0037 (HR, 2.359; 95% CI, 1.752-6.390) for overall survival (OS).

Conclusion: Machine learning systems could provide risk stratification for EOC patients before initial intervention through blood variables, including circulating tumor cells. The predictive algorithms could facilitate personalized treatment options through promising pre-treatment stratification of EOC patients.

Trial Registration: ChiCTR-DDD-16009601 Registered 25 October 2016.
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http://dx.doi.org/10.2147/OTT.S307546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140950PMC
May 2021

Multifrequency magnetic resonance elastography for elasticity quantitation and optimal tissue discrimination: A two-platform liver fibrosis mimicking phantom study.

NMR Biomed 2021 Aug 26;34(8):e4543. Epub 2021 May 26.

CEA, CNRS, Inserm, BioMaps, Université Paris-Saclay, Orsay, France.

In the framework of algebraic inversion, magnetic resonance elastography (MRE) repeatability, reproducibility and robustness were evaluated on extracted shear velocities (or elastic moduli). The same excitation system was implemented at two sites equipped with clinical MR scanners of 1.5 and 3 T. A set of four elastic, isotropic, homogeneous calibrated phantoms of distinct elasticity representing the spectrum of liver fibrosis severity was mechanically characterized. The repeatability of the measurements and the reproducibility between the two platforms were found to be excellent with mean coefficients of variations of 1.62% for the shear velocity mean values and 1.95% for the associated standard deviations. MRE velocities were robust to the amplitude and pattern variations of the displacement field with virtually no difference between outcomes from both magnets at identical excitation frequencies, even when the displacement field amplitude was six times smaller. However, MRE outcomes were very sensitive to the number of voxels per wavelength, s, of the recorded displacement field, with relative biases reaching 62% and precision loss by a factor of up to 23.5. For both magnetic field strengths, MRE accuracy and precision were largely degraded outside of established conditions of validity (6 ≲ s ≲ 9), resulting in estimated shear velocity values not significantly different between phantoms of increasing elasticity. When fulfilling the spatial sampling conditions, either prospectively in the acquisition or retrospectively before the reconstruction, MRE produced quantitative measurements that allowed to unambiguously discriminate, with infinitesimal p values, between the phantoms mimicking increasing severity of liver fibrosis.
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http://dx.doi.org/10.1002/nbm.4543DOI Listing
August 2021

Integrated Molecular Docking with Network Pharmacology to Reveal the Molecular Mechanism of Simiao Powder in the Treatment of Acute Gouty Arthritis.

Evid Based Complement Alternat Med 2021 27;2021:5570968. Epub 2021 Apr 27.

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

Background: The incidence of gout has been rapidly increasing in recent years with the changing of diet. At present, modern medications used in the clinical treatment of gout showed several side effects, such as gastrointestinal damage and the increased risk of cardiovascular disease. The traditional Chinese prescription Simiao Powder (SMP) has a long history in the treatment of acute gouty arthritis (AGA) and has a good curative effect. However, the mechanism and target of its therapeutic effects are still not completely understood.

Methods: Potential active compounds (PACs) and targets of SMP were found in the TCMSP database, and the disease target genes related to AGA were obtained by searching CTD, DisGeNET, DrugBank, GeneCards, TTD, OMIM, and PharmGKB disease databases with "acute gouty arthritis" and "Arthritis, Gouty" as keywords, respectively. The network of "Traditional Chinese medicine (TCM)-PACs-potential targets of acute gouty arthritis" was constructed with the Cytoscape 3.7.2 software, and the target genes of acute gouty arthritis were intersected with genes regulated by active compounds of SMP. The resultant common gene targets were input into Cytoscape 3.7.2 software, and the BisoGenet plug-in was used to construct a PPI network. The GO functional enrichment analysis and KEGG pathway enrichment analysis of the intersecting target proteins were performed using R software and corresponding program packages. The molecular docking verification was carried out between the potentially active compounds of SMP and the core target at the same time.

Results: 40 active components and 203 targets were identified, of which 95 targets were common targets for the drugs and diseases. GO function enrichment analysis revealed that SMP regulated several biological processes, such as response to lipopolysaccharide and oxidative stress, RNA polymerase II transcription regulator complex, protein kinase complex, and other cellular and molecular processes, including DNA-binding transcription factor binding. Results of KEGG pathway analysis showed that SMP was associated with AGA-related pathways such as interleukin-17 (IL-17), tumor necrosis factor (TNF), p53, and hypoxia-inducible factor 1 (HIF-1) signaling pathways. The results of molecular docking showed that active compounds in SMP exhibited strong binding to five core protein receptors (TP53, FN1, ESR1, CDK2, and HSPA5).

Conclusions: Active components of SMP, such as quercetin, kaempferol, wogonin, baicalein, beta-sitosterol, and rutaecarpine, showed therapeutic effects on AGA. These compounds were strongly associated with core target proteins (such as TP53, FN1, ESR1, CDK2, and HSPA5). This study reveals that IL-17, TNF, p53, and HIF-1 signaling pathways mediate the therapeutic effects of SMP on AGA. These findings expand our understanding of the mechanism of SMP in the treatment of AGA.
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http://dx.doi.org/10.1155/2021/5570968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100412PMC
April 2021

Transcriptome Analysis Provides Insights into the Mechanism of Astaxanthin Enrichment in a Mutant of the Ridgetail White Prawn .

Genes (Basel) 2021 Apr 21;12(5). Epub 2021 Apr 21.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

A mutant of the ridgetail white prawn, which exhibited rare orange-red body color with a higher level of free astaxanthin (ASTX) concentration than that in the wild-type prawn, was obtained in our lab. In order to understand the underlying mechanism for the existence of a high level of free astaxanthin, transcriptome analysis was performed to identify the differentially expressed genes (DEGs) between the mutant and wild-type prawns. A total of 78,224 unigenes were obtained, and 1863 were identified as DEGs, in which 902 unigenes showed higher expression levels, while 961 unigenes presented lower expression levels in the mutant in comparison with the wild-type prawns. Based on Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis, as well as further investigation of annotated DEGs, we found that the biological processes related to astaxanthin binding, transport, and metabolism presented significant differences between the mutant and the wild-type prawns. Some genes related to these processes, including crustacyanin, apolipoprotein D (ApoD), cathepsin, and cuticle proteins, were identified as DEGs between the two types of prawns. These data may provide important information for us to understand the molecular mechanism of the existence of a high level of free astaxanthin in the prawn.
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http://dx.doi.org/10.3390/genes12050618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143343PMC
April 2021

Antiangiogenic Effect of Platelet P2Y Inhibitor in Ischemia-Induced Angiogenesis in Mice Hindlimb.

Biomed Res Int 2021 8;2021:5529431. Epub 2021 Apr 8.

Clinical Laboratory, People's Hospital Affiliated to Jiangsu University, Zhenjiang 212002, China.

Purpose: Postischemic inflammation induces angiogenesis, while platelet P2Y inhibitors can alleviate this inflammation. Therefore, we studied the potential effects of P2Y inhibitor, ticagrelor, on angiogenesis in a mouse model of hindlimb ischemia.

Methods: Laser Doppler perfusion imaging and capillary density measurement were used for angiogenesis quantified. Immunofluorescence was used to detect the level of CD31. The mice muscle was harvested for enzyme-linked immunosorbent (ELISA) assay of interleukin- (IL) 10 activity and Western blot determination of vascular endothelial growth factor (VEGF) production.

Results: Ischemic hindlimb angiogenesis was sharply decreased in IL-10 mice than IL-10 mice. Ticagrelor inhibited angiogenesis and blood reperfusion recovery significantly elevated the levels of IL-10 and decreased the expression of VEGF in the IL-10 mouse ischemic hindlimb, which were abolished in IL-10-deficient (IL-10) C57BL/6J mice.

Conclusion: The study underscores that the effect of ticagrelor antiangiogenic function is related with the higher IL-10 expression.
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http://dx.doi.org/10.1155/2021/5529431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052144PMC
May 2021

Salivary Microbiome Variation in Early Childhood Caries of Children 3-6 Years of Age and Its Association With Iron Deficiency Anemia and Extrinsic Black Stain.

Front Cell Infect Microbiol 2021 23;11:628327. Epub 2021 Mar 23.

Department of Stomatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ECC is a common clinical manifestation of the oral cavity in childhood and Iron deficiency-anemia (IDA) is a high-risk factor but extrinsic black stain on the tooth surface is a protective factor for caries. There is limited information about oral microecological change in early children who suffer from ECC with IDA and extrinsic black stain (BS). This study enrolled 136 children aged 3-6 years. Dental caries and teeth BS were examined. Saliva was collected for 16S rRNA gene and fingertip blood were for Hemoglobin test. There are 93 ECC including 13 with IDA (IDA ECC) and 80 without IDA (NIDA ECC) and 43 caries free (CF) including 17 with BS (BSCF) and 26 without BS (NBS CF). Statistical analysis of microbiota data showed differences of the oral flora in different groups. The oral flora of the IDA ECC group had a high diversity, while the BSCF group had a low diversity. The bacterial genera Bacillus, Moraxella, and Rhodococcus were enriched in the IDA ECC while Neisseria was enriched in the NIDA ECC. Neisseria only exhibited high abundance in the BSCF, and the remaining genera exhibited high abundance in the NBSCF. Interestingly, the BSCF had the same trend as the NIDA ECC, and the opposite trend was observed with IDA ECC. We established random forest classifier using these biomarkers to predict disease outcomes. The random forest classifier achieved the best accuracy in predicting the outcome of caries, anemia and black stain using seven, one and eight biomarkers, respectively; and the accuracies of the classifiers were 93.35%, 94.62% and 95.23%, respectively. Our selected biomarkers can achieve good prediction, suggesting their potential clinical implications.
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http://dx.doi.org/10.3389/fcimb.2021.628327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044945PMC
July 2021

A The complete chloroplast genome and phylogenetic analysis of Bupleurum yinchowense Shan & Yin Li.

Mitochondrial DNA B Resour 2021 Mar 30;6(3):1233-1235. Epub 2021 Mar 30.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Shan & Yin Li was first described as a new species in 1974, but its classification status has always been disputed. Here, its complete chloroplast genome was provided to resolve this issue. The length of the chloroplast genome is 155,851 bp and composed of two inverted repeats (IR: 26,307 bp), a large single-copy region (LSC: 85,625 bp), and a small single-copy region (SSC: 17,612 bp). The overall GC content is 37.6%. The chloroplast genome consists of 113 genes, including 79 protein-coding genes, four rRNA genes, and 30 tRNA genes. Phylogenetic analysis suggested that holds a distinct phylogenetic position and can be considered as an accepted species.
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http://dx.doi.org/10.1080/23802359.2020.1866465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018508PMC
March 2021

Correction to: LncRNA APCDD1L-AS1 induces icotinib resistance by inhibition of EGFR autophagic degradation via the miR-1322/miR-1972/ miR-324-3p-SIRT5 axis in lung adenocarcinoma.

Biomark Res 2021 Apr 14;9(1):25. Epub 2021 Apr 14.

Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China.

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http://dx.doi.org/10.1186/s40364-021-00279-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048049PMC
April 2021

Virtual Surgical Planning and Three-Dimensional Printing to Aid the Anatomical Reduction of an Old Malunited Fracture of the Mandible.

J Craniofac Surg 2021 Mar 25. Epub 2021 Mar 25.

Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao School of Stomatology, Qingdao University, Qingdao Shandong, China.

Abstract: Mandible fracture is a common injury in maxillofacial surgery. It causes not only maxillofacial dysfunction but also facial deformities. Malunited fractures of the mandible have been a vast challenge in clinical treatment due to the misalignment of the broken ends and the occurrence of occlusal disorders. This case report describes using virtual surgical planning and three-dimensional printing to treat a patient with malunited fracture of the mandible. Failing to perform mandibular surgery due to severe brain trauma after the car accident, the patient got malunited healing of mandible. The authors applied virtual surgical planning to perform preoperative analysis and surgical design on this patient, three-dimensional printing to fabricate occlusal plate, and models of the preoperative and postoperative mandible to guide the operation. Finally, the authors achieved the reduction and reconstruction of the mandible with satisfactory clinical results.
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http://dx.doi.org/10.1097/SCS.0000000000007653DOI Listing
March 2021

Phenylpropanoid-conjugated pentacyclic triterpenoids from the whole plants of Leptopus lolonum induced cell apoptosis via MAPK and Akt pathways in human hepatocellular carcinoma cells.

Bioorg Chem 2021 Jun 1;111:104886. Epub 2021 Apr 1.

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address:

Our present and previous phytochemical investigations on Leptopus lolonum have resulted in the isolation of almost 30 phenylpropanoid-conjugated pentacyclic triterpenoids (PCPTs). During the continuous study on PCPTs, this kind of triterpenoid ester is considered as a natural product with low toxicity because of it's widely distribution in natural plants and edible fruits including kiwi fruit, durian, jujube, pawpaw, apple and pear. In the present work, we report the isolation, structural elucidation and cytotoxic evaluation of four new PCPTs (1-4) which obtained from L. lolonum. In addition, the possible biosynthesis pathway for 28-norlupane triterpenoid and potent effect of phenylpropanoid moiety for increasing the cytotxic effect of triterpenoids were also discussed. Among these compounds, compound 1 exhibited the highest cytotoxic effect on HepG2 cells with IC value of 11.87 μM. Further flow cytometry and western blot analysis demonstrated that 1 caused G1 cell cycle arrest by up-regulated the expression of phosphorylated p53 protein in HepG2 cells and induced cell apoptosis via MAPK and Akt pathways. These results emphasized the potential of PCPTs as lead compounds for developing anti-cancer drugs.
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http://dx.doi.org/10.1016/j.bioorg.2021.104886DOI Listing
June 2021

Low Expression of Circular RNA hsa_circ_0078607 Predicts Poor Prognosis in High-Grade Serous Ovarian Cancer.

Cancer Manag Res 2021 29;13:2877-2883. Epub 2021 Mar 29.

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China.

Objective: Recent studies have shown that circRNAs participate in ovarian cancer progression and act as potential biomarkers for ovarian cancer diagnosis and prognosis. In the present study, we aimed to investigate the expression pattern and prognostic significance of circ_0078607 in high-grade serous ovarian cancer (HGSOC).

Methods: The expression of circ_0078607 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in 49 cases of HGSOC. Clinical data of patients with HGSOC were retrospectively collected, and those patients were divided according to their expression of circ_0078607. Correlation between circ_0078607 and clinical features as well as the prognosis in patients with HGSOC was analyzed. -test and chi-square test were used to compare continuous and categorical variables. The Cox hazard regression model was used to assess prognostic factors. Both progression-free survival (PFS) and overall survival (OS) curves were generated by Kaplan-Meier method.

Results: The expression of circ_0078607 was significantly downregulated in ovarian cancer tissues compared with adjacent non-cancerous tissues. Besides, patients with low circ_0078607 expression exhibited parameters associated with poor prognosis, including advanced FIGO stage and higher serum CA125 level. Kaplan-Meier survival curve analysis showed that patients with low circ_0078607 expression had shorter PFS and OS. Cox regression analysis showed that low expression of circ_0078607 was a predictor for poor PFS and OS in HGSOC patients.

Conclusion: Low expression of circ_0078607 might be an adverse prognostic indicator for HGSOC.
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http://dx.doi.org/10.2147/CMAR.S300738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020458PMC
March 2021

Horizontally acquired cysteine synthase genes undergo functional divergence in lepidopteran herbivores.

Heredity (Edinb) 2021 Jul 8;127(1):21-34. Epub 2021 Apr 8.

College of Animal Sciences, Zhejiang University, Hangzhou, China.

Horizontal gene transfer (HGT) plays an important role in evolutionary processes as organisms adapt to their environments, and now cases of gene duplication after HGT in eukaryotes are emerging at an increasing rate. However, the fate and roles of the duplicated genes over time in eukaryotes remain unclear. Here we conducted a comprehensive analysis of the evolution of cysteine synthase (CYS) in lepidopteran insects. Our results indicate that HGT-derived CYS genes are widespread and have undergone duplication following horizontal transfer in many lepidopteran insects. Moreover, lepidopteran CYS proteins not only have β-cyanoalanine synthase activity but also possess cysteine synthase activity that is involved in sulfur amino acid biosynthesis. Duplicated CYS genes show marked divergence in gene expression patterns and enzymatic properties, suggesting that they probably have undergone subfunctionalization and/or neofunctionalization in Lepidoptera. The gene transfer of CYS genes and subsequent duplication appears to have facilitated the adaptation of lepidopteran insects to different diets and promoted their ecological diversification. Overall, this study provides valuable insights into the ecological and evolutionary contributions of CYS in lepidopteran insects.
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http://dx.doi.org/10.1038/s41437-021-00430-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249628PMC
July 2021

Function and evolution of allelic variations of Sr13 conferring resistance to stem rust in tetraploid wheat (Triticum turgidum L.).

Plant J 2021 Jun 29;106(6):1674-1691. Epub 2021 May 29.

USDA-ARS, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, Fargo, ND, 58102, USA.

The resistance gene Sr13 is one of the most important genes in durum wheat for controlling stem rust caused by Puccinia graminis f. sp. tritici (Pgt). The Sr13 functional gene CNL13 has haplotypes R1, R2 and R3. The R1/R3 and R2 haplotypes were originally designated as alleles Sr13a and Sr13b, respectively. To detect additional Sr13 alleles, we developed Kompetitive allele specific PCR (KASP™) marker KASPSr13 and four semi-thermal asymmetric reverse PCR markers, rwgsnp37-rwgsnp40, based on the CNL13 sequence. These markers were shown to detect R1, R2 and R3 haplotypes in a panel of diverse tetraploid wheat accessions. We also observed the presence of Sr13 in durum line CAT-A1, although it lacked any of the known haplotypes. Sequence analysis revealed that CNL13 of CAT-A1 differed from the susceptible haplotype S1 by a single nucleotide (C2200T) in the leucine-rich repeat region and differed from the other three R haplotypes by one or two additional nucleotides, confirming that CAT-A1 carries a new (R4) haplotype. Stem rust tests on the monogenic, transgenic and mutant lines showed that R1 differed from R3 in its susceptibility to races TCMJC and THTSC, whereas R4 differed from all other haplotypes for susceptibility to TTKSK, TPPKC and TCCJC. Based on these differences, we designate the R1, R3 and R4 haplotypes as alleles Sr13a, Sr13c and Sr13d, respectively. This study indicates that Sr13d may be the primitive functional allele originating from the S1 haplotype via a point mutation, with the other three R alleles probably being derived from Sr13d through one or two additional point mutations.
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http://dx.doi.org/10.1111/tpj.15263DOI Listing
June 2021

Expression of Serum miR-155 in Children with Pneumonia and Its Role in Immunity to .

Infect Drug Resist 2021 29;14:1273-1281. Epub 2021 Mar 29.

Department of Pediatrics, Affiliated Shuyang Hospital of Xuzhou Medical University, Shuyang, 223600, Jiangsu, People's Republic of China.

Objective: To investigate the expression of serum miR-155 in children with pneumonia (MPP).

Methods: A total of 100 children at our hospital with pneumonia caused by infection were enrolled as a study group, including 45 cases in the acute phase (acute phase group) and 55 in the recovery phase (recovery phase group). An additional 30 healthy children were enrolled during the same period as the control group. The expression levels of miR-155, tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), IL-10, IL-13, immunoglobulin (Ig) G, IgA, complements (C3 and CH50), and T lymphocyte subsets (CD , CD , CD , and CD /CD ) were determined. Multivariate logistic regression analysis was performed to identify risk factors affecting MPP in children.

Results: miR-155, IL-10, IgG, IgA, CD , CD , and CD /CD were poorly expressed in children with MPP, and their expression in the acute phase group was significantly lower than that in the recovery phase group. TNF-α, IL-13, C3, and CH50 were highly expressed in the children, and their expression was significantly higher in the acute  phase group than in the recovery phase group. In the acute phase group, the expression of IL-8 was significantly higher than that in the control and recovery phase groups but without any significant differences between the recovery phase and control groups. Age, season, low complement state, epidemiological contact history, and antibiotic use time were independent risk factors affecting MPP in children.

Conclusion: Serum miR-155 is poorly expressed in children with MPP, and it can regulate inflammatory disorders and immune responses.
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http://dx.doi.org/10.2147/IDR.S273423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018411PMC
March 2021

Optimization of High Hydrostatic Pressure Treatments on Soybean Protein Isolate to Improve Its Functionality and Evaluation of Its Application in Yogurt.

Foods 2021 Mar 20;10(3). Epub 2021 Mar 20.

Bor S. Luh Food Safety Research Center, SJTU-OSU Innovation Center for Environmental Sustainability, Key Laboratory of Urban Agriculture, Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.

This work aimed to improve the functional properties of soybean protein isolate (SPI) by high hydrostatic pressure (HHP) and develop SPI incorporated yogurt. Response surface methodology (RSM) was used to optimize the HHP treatment parameters, including pressure, holding time, and the ratio of SPI/water. Water holding capacity, emulsifying activity index, solubility, and hardness of SPI gels were evaluated as response variables. The optimized HPP treatment conditions were 281 MPa of pressure, 18.92 min of holding time, and 1:8.33 of SPI/water ratio. Water and oil holding capacity, emulsifying activity, and stability of SPI at different pH were improved. Additionally, relative lipoxygenase (LOX) activity of HHP treated SPI (HHP-SPI) was decreased 67.55 ± 5.73%, but sulphydryl group content of HHP-SPI was increased 12.77%, respectively. When incorporating 8% of SPI and HHP-SPI into yogurt, the water holding capacity and rheological properties of yogurt were improved in comparison with yogurt made of milk powders. Moreover, HHP-SPI incorporated yogurt appeared better color and flavor.
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http://dx.doi.org/10.3390/foods10030667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003865PMC
March 2021

Seroprevalence investigation and genetic analysis of pseudorabies virus within pig populations in Henan province of China during 2018-2019.

Infect Genet Evol 2021 Aug 31;92:104835. Epub 2021 Mar 31.

Zhengzhou Major Pig Disease Prevention and Control Laboratory, College of Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou 450046, People's Republic of China. Electronic address:

In late 2011, the outbreak of pseudorabies (PR) occurred in Bartha-K61-vaccinated pig farms and spread rapidly to many provinces of China, causing substantial economic losses to the swine industry. A total of 4708 pig serum samples from Henan province during 2018-2019 were collected to screen for the presence of pseudorabies virus (PRV) gE-specific antibodies, and phylogenetic analysis based on the gE gene of PRV was performed. Of the 4708 serum samples tested, 30.14% (1419/4708) were seropositive for PRV antibodies, based on PRV gE-coated enzyme-linked immunosorbent assay (ELISA), with slaughterhouses having the highest seroprevalence. The seropositive rates of PRV also varied with the region and the season. Phylogenetic analysis showed that three PRV isolates from this study were clustered in an independent branch together with the Chinese variant PRV strains (after 2012), and had a closer genetic relationship with the Chinese variant PRV strains, but differed genetically from the 4 early Chinese PRV strains and 4 European-American strains. This study suggests that three PRV isolates may belong to PRV variants, and the development of a novel vaccine against PRV variants is particularly urgent.
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http://dx.doi.org/10.1016/j.meegid.2021.104835DOI Listing
August 2021

Development and validation for prognostic nomogram of epithelial ovarian cancer recurrence based on circulating tumor cells and epithelial-mesenchymal transition.

Sci Rep 2021 Mar 22;11(1):6540. Epub 2021 Mar 22.

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

We aimed to determine the prognosis value of circulating tumor cells (CTCs) undergoing epithelial-mesenchymal transition in epithelial ovarian cancer (EOC) recurrence. We used CanPatrol CTC-enrichment technique to detect CTCs from blood samples and classify subpopulations into epithelial, mesenchymal, and hybrids. To construct nomogram, prognostic factors were selected by Cox regression analysis. Risk stratification was performed through Kaplan-Meier analysis among the training group (n = 114) and validation group (n = 38). By regression screening, both CTC counts (HR 1.187; 95% CI 1.098-1.752; p = 0.012) and M-CTC (HR 1.098; 95% CI 1.047-1.320; p = 0.009) were demonstrated as independent factors for recurrence. Other variables including pathological grade, FIGO stage, lymph node metastasis, ascites, and CA-125 were also selected (p < 0.005) to construct nomogram. The C-index of internal and external validation for nomogram was 0.913 and 0.874. We found significant predictive values for the nomogram with/without CTCs (AUC 0.8705 and 0.8097). Taking CTC counts and M-CTC into separation, the values were 0.8075 and 0.8262. Finally, survival curves of risk stratification based on CTC counts (p = 0.0241), M-CTC (p = 0.0107), and the nomogram (p = 0.0021) were drawn with significant differences. In conclusion, CTCs could serve as a novel factor for EOC prognosis. Nomogram model constructed by CTCs and other clinical parameters could predict EOC recurrence and perform risk stratification for clinical decision-making.Trial registration Chinese Clinical Trial Registry, ChiCTR-DDD-16009601, October 25, 2016.
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http://dx.doi.org/10.1038/s41598-021-86122-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985206PMC
March 2021

Posttraumatic growth in Chinese nurses and general public during the COVID-19 outbreak.

Psychol Health Med 2021 Mar 16:1-11. Epub 2021 Mar 16.

Department of Urology, The Changzhou Second People's Hospital Affiliated of Nanjing Medical University, Changzhou, China.

COVID-19 as a pandemic disease, till 18 May 2020, has infected more than 84,494 people in China and 4721,051 abroad. While countries around the world concentrate on controlling the pandemic to minimize damage to this country, the positive psychology brought to nurses and general public (GP) by COVID-19 should not be ignored. This study aims to assess post-traumatic growth (PTG) of Chinese nurses and GP during the COVID-19 pandemic. The study employed PTG in Chinese nurses and GP with Posttraumatic growth inventory questionnaire (PTGI) via a mobile app-based questionnaire, anxiety and ways to copy with stress in nurses were also employed. A total of 455 nurses and 424 GP were included in the statistical analysis. Results indicated that score of total PTGI and three dimensions, new possibilities, personal strength and spiritual change, were different between nurses and GP. Furthermore, score of total PTGI and all domains were significantly different between 178 front-line nurses (FLNs) and 277 non-front-line nurses (nFLNs). Importantly, sex, marriage status, professional titles, fertility, anxiety and ways to copy with stress were associated with PTG in nurses. Moreover, marriage status and ways to copy with stress were the predictors of PTG in nurses. Interestingly, this study found that WeChat network psychological counseling and phone app of application self-relaxation were good and effective coping strategies for nurses to relieve stress. Thus, the development of valid intervention programs for nurses to diminish job burnout and increase care quality was also important.
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http://dx.doi.org/10.1080/13548506.2021.1897148DOI Listing
March 2021

Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation.

Front Cell Dev Biol 2021 18;9:622198. Epub 2021 Feb 18.

Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, China.

Succinylation is a newly discovered and multienzyme-regulated post-translational modification (PTM) that is associated with the initiation and progression of cancer. Currently, no systematic analyses on the role of succinylation regulators in tumors have been reported. In this study, we performed a comprehensive pan-cancer analysis on four well-known succinylation regulators (CPT1A, KAT2A, SIRT5, and SIRT7). We found that these regulators played specific and critical roles in the prognosis of clear cell renal cell carcinoma (ccRCC). We constructed a risk score (RS) based on two independent prognostic prediction factors, CPT1A and KAT2A, and subsequently developed a nomogram model containing the RS, which showed good accuracy in the prediction of overall survival (OS) in ccRCC patients. Furthermore, we used the similar expression pattern of four succinylation regulators according to consensus clustering analysis to divide the patients into three clusters that exhibited prominently different OS as well as clinicopathological characteristics. Differently expressed genes (DEGs) and pathway enrichment analyses of three clusters indicated that succinylation regulators might promote malignant progression of ccRCC by regulating the infiltration of immune cells and RNA N6-methyladenosine (m6A) methylation. Importantly, our data suggest that CPT1A and SIRT5 might up-regulate and down-regulate the expression of LRPPRC and EIF3B, respectively. Our study systematically analyzed the prognostic predictive values of four succinylation regulators and revealed their potential mechanisms in ccRCC aggressiveness. These data provide new insight into the understanding of succinylation modification and present clinical evidence for its role in ccRCC treatments.
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http://dx.doi.org/10.3389/fcell.2021.622198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935513PMC
February 2021

Treatment of oral lichen planus by surgical excision and acellular dermal matrix grafting: Eleven case reports and review of literature.

World J Clin Cases 2021 Feb;9(6):1446-1454

Department of Stomatology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.

Background: Oral lichen planus (OLP) is a chronic inflammatory disorder, and it can affect normal oral function. The conventional treatments for OLP are not always effective, and relapse easily occurs. Therefore, treatment of OLP is difficult and challenging. In this study, we evaluated over a long period the clinical efficacy of surgical excision and acellular dermal matrix (ADM) grafting in patients with refractory OLP.

Case Summary: Eleven patients with refractory OLP underwent a standardized protocol of surgical excision and ADM grafting. The condition of the area of the grafted wound, the intraoperative maximum mouth opening, pain, and clinical healing were assessed at postoperative follow-up visits. All patients had a flat surgical area with similar mucosal tissue coverage and local scar formation. Patients had no irritation and pain in their mucous membranes when eating acidic and spicy food. All patients' mouth openings returned to normal within 2-6 mo after surgery. During follow-up, none of the patients had recurrence of OLP after surgery. The longest follow-up was 11 yr and the shortest was 6 mo, and none of the patients relapsed during follow-up.

Conclusion: Surgical excision and ADM grafting could be an effective method to treat refractory OLP.
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http://dx.doi.org/10.12998/wjcc.v9.i6.1446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896670PMC
February 2021

Membrane engineering of cell membrane biomimetic nanoparticles for nanoscale therapeutics.

Clin Transl Med 2021 02;11(2):e292

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

In recent years, cell membrane camouflaging technology has emerged as an important strategy of nanomedicine, and the modification on the membranes is also a promising approach to enhance the properties of the nanoparticles, such as cancer targeting, immune evasion, and phototherapy sensitivity. Indeed, diversified approaches have been exploited to re-engineer the membranes of nanoparticles in several studies. In this review, first we discuss direct modification strategy of cell membrane camouflaged nanoparticles (CM-NP) via noncovalent, covalent, and enzyme-involved methods. Second, we explore how the membranes of CM-NPs can be re-engineered at the cellular level using strategies such as genetic engineering and membranes fusion. Due to the innate biological properties and excellent biocompatibility, the functionalized cell membrane-camouflaged nanoparticles have been widely applied in the fields of drug delivery, imaging, detoxification, detection, and photoactivatable therapy.
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http://dx.doi.org/10.1002/ctm2.292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819108PMC
February 2021

Two indigenous Berberis species from Spain were confirmed as alternate hosts of the yellow rust fungus Puccinia striiformis f.sp. tritici.

Plant Dis 2021 Feb 25. Epub 2021 Feb 25.

Aarhus Universitet, 1006, Agroecology, Forsoegsvej 1, Slagelse, Denmark, 4200;

Puccinia striiformis f.sp. tritici (Pst), which causes yellow (or stripe) rust on wheat, is a macrocyclic and heteroecious fungus. In this study, we investigated whether Berberis vulgaris subsp. seroi and B. vulgaris subsp. australis, which are indigenous in Spain, may serve as alternate hosts for Pst. Wheat leaves bearing telia of an isolate of Pst were harvested and used to inoculate plants of both barberry subspecies. Pycnia were observed on the adaxial side of the leaves from 10 days after inoculation (dai). Following successful fertilisation, aecia were observed on the abaxial side of the leaves from 16 dai. At 27 dai, barberry leaves bearing aecia were detached and used to inoculate susceptible wheat seedlings of cultivar 'Morocco'. Uredinia were observed on wheat seedlings from 12 days after aeciospore exposure. Eighty-three single lesions were recovered from individual wheat leaves, of which 43 were genotyped using 19 Pst simple sequence repeat markers (SSR). In total, 19 multilocus genotypes (MLGs) were identified among the 43 progeny isolates. The SSR genotyping confirmed that all 43 isolates were derived from the parental isolate. Seven heterozygous SSR markers showed segregation among the progenies, whereas none of the 12 homozygous markers resulted in segregation. These results demonstrated that B. vulgaris subspp. seroi and australis can serve as alternate hosts for the yellow rust fungus, which may result in novel virulence combinations that can have a detrimental impact on wheat production. Although Pst has not been detected on these barberry species in nature, this study highlights the importance of rust surveillance in barberry areas where suitable conditions for completion of the sexual life cycle may be present.
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http://dx.doi.org/10.1094/PDIS-02-21-0269-SCDOI Listing
February 2021
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