Publications by authors named "Yuchuan Sun"

7 Publications

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Curcumin Reduces Neuroinflammation and Improves the Impairments of Anesthetics on Learning and Memory by Regulating the Expression of miR-181a-5p.

Neuroimmunomodulation 2021 Apr 13:1-9. Epub 2021 Apr 13.

Department of Anesthesiology, Laiyang Central Hospital, Yantai City, China.

Objective: The purpose of this study was to explore the role of curcumin (Cur) in isoflurane (ISO)-induced learning and memory dysfunction in Sprague-Dawley rats and further elucidate the mechanism of the protective effect produced by Cur.

Methods: Rat models of cognitive impairment were established by inhaling 3% ISO. The Morris water maze test was used to assess the cognitive function of rats. ELISA and qRT-PCR were used to analyze the protein levels of pro-inflammatory cytokines and expression levels of miR-181a-5p, respectively.

Results: Cur significantly improved the ISO-induced cognitive dysfunction in rats and alleviated the ISO-induced neuroinflammation. miR-181a-5p was overexpressed in ISO-induced rats, while Cur treatment significantly reduced the expression of miR-181a-5p. Overexpression of miR-181a-5p promoted the cognitive impairment and the release of inflammatory cytokines and reversed the neuroprotective effect of Cur.

Conclusion: Cur has a protective effect on ISO-induced cognitive dysfunction, which may be achieved by regulating the expression of miR-181a-5p.
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http://dx.doi.org/10.1159/000514548DOI Listing
April 2021

The distribution of liver cancer stem cells correlates with the mechanical heterogeneity of liver cancer tissue.

Histochem Cell Biol 2021 Mar 12. Epub 2021 Mar 12.

Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Chongqing University, Ministry of Education, Chongqing, 400030, People's Republic of China.

The survival of cancer stem cells is usually limited to a specific tumor microenvironment, and this microenvironment plays a vital role in the development of tumors. The mechanical properties of the microenvironment differ in different regions of solid tumors. However, in solid tumors, whether the distribution of cancer stem cells relates to the mechanical microenvironment of different regions is still unclear. In this study, we undertook a biophysical and biochemical assessment of the changes in the mechanical properties of liver tissue during the progression of liver cancer and explored the distribution of liver cancer stem cells in liver cancer tissues. Our analysis confirmed previous observations that the stiffness of liver tissue gradually increased with the progress of fibrosis. In liver cancer tissues, we found obvious mechanical heterogeneity: the core of the tumor was soft, the invasive front tissue was the hardest, and the para-cancer tissue was in an intermediate state. Interestingly, the greatest number of liver cancer stem cells was found in the invasive front part of the tumor. We finally established that stroma stiffness correlated with the number of liver cancer stem cells. These findings indicate that the distribution of liver cancer stem cells correlates with the mechanical heterogeneity of liver cancer tissue. This result provides a theoretical basis for the development of targeted therapies against the mechanical microenvironment of liver cancer stem cells.
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http://dx.doi.org/10.1007/s00418-021-01979-wDOI Listing
March 2021

Characteristics and ecological risk assessment of polycyclic aromatic hydrocarbons in soil seepage water in karst terrains, southwest China.

Ecotoxicol Environ Saf 2020 Mar 31;190:110122. Epub 2019 Dec 31.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, 01003, United States.

Concentrations of polycyclic aromatic hydrocarbons (PAHs) in soils and soil seepage waters were analyzed along with dissolved organic carbon (DOC) to investigate the ecological risks and factors controlling the subsurface transport of PAHs in karst terrain in southwest China. The concentrations of dissolved PAHs in soil seepage water increased with depth and the mean concentrations at a depth of 80 cm were relatively high (exceeding 1147 ng/L). PAH composition in soil seepage water was dominated by low molecular weight (LMW) PAHs, whereas those associated with soil matrix were dominated by high molecular weight (HMW) PAHs. The results revealed that HMW PAHs in soil seepage water were closer to the equilibrium of dissolution than LMW and medium molecular weight (MMW) PAHs. However, due to the carrier functions of dissolved organic matter (DOM) for HMW PAHs, all PAHs can be continuously dissolved in the soil seepage water from soil matrix as the water moved vertical downward through the profiles. During this process, dynamic sorption and desorption processes were occurring between constituents in the soil seepage water and the soil matrix. This study demonstrates soil seepage water has posed a high risk to the groundwater, and effective protection is urgently needed.
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http://dx.doi.org/10.1016/j.ecoenv.2019.110122DOI Listing
March 2020

Vertical migration from surface soils to groundwater and source appointment of polycyclic aromatic hydrocarbons in epikarst spring systems, southwest China.

Chemosphere 2019 Sep 8;230:616-627. Epub 2019 May 8.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, 01003, United States.

Understanding the transfer process of polycyclic aromatic hydrocarbons (PAHs) in the karst terrain is of great importance to their ecological risk assessments, however, the impact of the vertical transfer of the soil PAHs on the underground water is largely unknown in the karst system. Here, the vertical distribution and the seasonal variation of 16 PAHs in the soils and the water of 4 epikarst spring catchments in Southwest China were investigated. The total concentration of the PAHs ranged within 61-3285 ng g in the soils, and 341-4969 ng L in the spring water. The vertical distribution of the PAHs in soils varied with ring numbers and altitude of the catchment. PAHs concentrations were linearly related with the total organic carbon (TOC) at different depths in the catchments 563-783 m above the sea level (A.S.L.). However, no correlation with TOC was observed in the catchment of a high altitude (2090 m A.S.L.), because the large water flux led to the fast migration of the 2-3 rings PAHs in soils. The PAHs in soils and springs were mainly derived from the combustion of grass/wood/coal, closely related with the primary fossil fuels used in this area. This study demonstrate that the groundwater was heavily polluted by PAHs in the karst terrains of Southwest China, due to the vertical transfer of PAHs from the surface soils, and effective protection was urgently needed.
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http://dx.doi.org/10.1016/j.chemosphere.2019.05.007DOI Listing
September 2019

Transport of polycyclic aromatic hydrocarbons in a highly vulnerable karst underground river system of southwest China.

Environ Sci Pollut Res Int 2018 Dec 11;25(34):34519-34530. Epub 2018 Oct 11.

Chongqing Key Laboratory of Karst Environment, School of Geographical Science, Southwest University, Chongqing, 400715, China.

The concentration and fluxes of polycyclic aromatic hydrocarbons (PAHs) were investigated in a karst underground river system in southwest China. Groundwater, particles, and sediments from underground river, topsoil, and surface water were monitored, allowing establishment of a conceptual model of PAH transport at the watershed scale. The results showed that PAHs could be transported from the surface to the subsurface through two migration pathways, which were slow-flowing water in the karst fissure and fast-flowing water in conduits. During rainfall events, increasing PAH levels (concentrations and fluxes) at the underground river exit indicated that hydrodynamic force could facilitate PAH transport. The PAHs in water were dominated by dissolved PAHs, accounting for 58.7% of total, especially in the freely dissolved phase, in which SPM-associated PAHs accounted for 41.3% of the total PAHs. Low molecular weight PAHs dominated transport and were mainly transported in dissolved form, whereas high molecular weight PAHs were dominated by SPM-associated transport during the rainfall events. A significantly positive correlation was observed between two-ring and three-ring freely dissolved PAHs and dissolved organic carbon (p < 0.01), respectively. Moreover, PAHs with four to five rings were relatively more abundant in the dissolved organic matter (DOM) associated phase than in the freely dissolved phase, suggesting a major role of DOM in their transport during rainfall events. The trend of PAH fluxes suggested that particle-facilitated transport was another dominant cause of PAH mobilization.
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http://dx.doi.org/10.1007/s11356-018-3005-zDOI Listing
December 2018

Simulation of the transfer and fate of γ-HCH in epikarst system.

Chemosphere 2016 Apr 23;148:255-62. Epub 2016 Jan 23.

Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Geographical Sciences, Southwest University, Chongqing 400715, China; Karst Dynamics Laboratory, Ministry of Land and Resources, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

The thin surface soil layer and karst features in karst terrains lead to poor filtration, poor pre-purification and rapid infiltration, so that karst groundwater systems are particularly vulnerable to contamination. Due to its extensive use in past, gamma-hexachlorocyclohexane (γ-HCH) is ubiquitous in various environmental compartments of China, even though it has been prohibited since 1984. However, very little is known about its movement and behavior in special karst system. In this study, a dynamic fugacity model was established for γ-HCH in epikarst system via dividing the karst soil into multiple layers coupled with the physical-chemical properties of γ-HCH. The simulated results in soil profile were in good agreement with the measured values of γ-HCH. The modeled results predict that only 18 g γ-HCH will be left in the studied area in 2020, which is only 0.4% of the largest reserves in 1983, and about 99.99% of γ-HCH will remains in soil. The concentrations of γ-HCH in air, plant and 0-20 cm layer soil in the studied area descended quickly after HCHs was prohibited in 1984, while its concentration in soil layer deeper than 20 cm (deeper soil) increased continuously till 1997. The dominant transfer process of γ-HCH between the adjacent compartments in the studied area was from 0-20 cm layer to the deeper soil. Sensitivity analysis results showed that emission rate, infiltration coefficient, total organic carbon of soil, degradation rate in soil, compartment area and volume were the top six influential parameters for predicting γ-HCH concentration.
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http://dx.doi.org/10.1016/j.chemosphere.2015.10.091DOI Listing
April 2016

Biodegradation of nitrobenzene in a lysogeny broth medium by a novel halophilic bacterium Bacillus licheniformis.

Mar Pollut Bull 2014 Dec 14;89(1-2):384-389. Epub 2014 Oct 14.

College of Resources and Environment, Southwest University, Chongqing 400715, PR China.

The Bacillus licheniformis strain YX2, a novel nitrobenzene-degrading halophilic bacterium, was isolated from active sludge obtained from a pesticide factory. Strain YX2 can withstand highly acidic and alkaline conditions and high temperatures. Degradation of nitrobenzene (200mgL(-1)) by YX2 exceeded 70% after 72h in lysogeny broth medium (pH 4-9). Under optimal degradation conditions (33°C, pH 7 in LB medium) YX2 degraded 50, 100, 200, and 600mgL(-1) nitrobenzene within 36, 36, 72, and 156h, respectively. Even in the presence of benzene, phenol or aniline, strain YX2 efficiently degraded nitrobenzene. Furthermore, strain YX2 completely degraded 600mgL(-1) nitrobenzene in 7% NaCl (w/w). Thus, our data show that strain YX2 may have promise for removing nitrobenzene from complex wastewaters with high salinity and variable pH.
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http://dx.doi.org/10.1016/j.marpolbul.2014.09.028DOI Listing
December 2014