Publications by authors named "Yucheng Zhang"

97 Publications

Prognostic Value of Transfer Learning Based Features in Resectable Pancreatic Ductal Adenocarcinoma.

Front Artif Intell 2020 5;3:550890. Epub 2020 Oct 5.

Department of Medical Imaging, University of Toronto, Toronto, ON, Canada.

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most aggressive cancers with an extremely poor prognosis. Radiomics has shown prognostic ability in multiple types of cancer including PDAC. However, the prognostic value of traditional radiomics pipelines, which are based on hand-crafted radiomic features alone is limited. Convolutional neural networks (CNNs) have been shown to outperform radiomics models in computer vision tasks. However, training a CNN from scratch requires a large sample size which is not feasible in most medical imaging studies. As an alternative solution, CNN-based transfer learning models have shown the potential for achieving reasonable performance using small datasets. In this work, we developed and validated a CNN-based transfer learning model for prognostication of overall survival in PDAC patients using two independent resectable PDAC cohorts. The proposed transfer learning-based prognostication model for overall survival achieved the area under the receiver operating characteristic curve of 0.81 on the test cohort, which was significantly higher than that of the traditional radiomics model (0.54). To further assess the prognostic value of the models, the predicted probabilities of death generated from the two models were used as risk scores in a univariate Cox Proportional Hazard model and while the risk score from the traditional radiomics model was not associated with overall survival, the proposed transfer learning-based risk score had significant prognostic value with hazard ratio of 1.86 (95% Confidence Interval: 1.15-3.53, -value: 0.04). This result suggests that transfer learning-based models may significantly improve prognostic performance in typical small sample size medical imaging studies.
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http://dx.doi.org/10.3389/frai.2020.550890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861273PMC
October 2020

LINC00665 functions as a competitive endogenous RNA to regulate AGTR1 expression by sponging miR‑34a‑5p in glioma.

Oncol Rep 2021 Mar 22;45(3):1202-1212. Epub 2021 Jan 22.

Department of General Surgery, Wenzhou Hospital Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang 325000, P.R. China.

Glioma is the most aggressive tumor of the central nervous system. Long non‑coding RNAs (lncRNAs) may be involved in modulating tumor generation. The present study analyzed an lncRNA microarray of glioma and selected long intergenic non‑protein coding RNA 665 (LINC00665) as the research object. The mode of expression and biological function of LINC00665 in glioma were assessed using lncRNA microarray and RT‑qPCR analyses. Gain‑of‑function assays and/or loss‑of‑function assays were implemented to explore the role of LINC00665 in the progression of glioma. Dual‑luciferase reporter and RNA immunoprecipitation assays explored the downstream molecular mechanism of LINC00665. The function of the molecular pathway in progression of glioma was analyzed using rescue assays. High expression of LINC00665 was marked in glioma tissues and cells, which correlated with an unsatisfactory prognosis. Upregulation of LINC00665 significantly promoted the proliferation and invasion of glioma cells. LINC00665 acted as a competing endogenous RNA by sponging miR‑34a‑5p to upregulate angiotensin II receptor type 1 (AGTR1). LINC00665 promoted the progression of glioma by acting as a competitive endogenous RNA to competitively bind to miR‑34a‑5p and mediate AGTR1 expression.
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http://dx.doi.org/10.3892/or.2021.7949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859982PMC
March 2021

Genome sequence of Monilinia vaccinii-corymbosi sheds light on mummy berry disease infection of blueberry and mating type.

G3 (Bethesda) 2021 Feb;11(2)

Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695, USA.

Mummy berry disease, caused by the fungal pathogen Monilinia vaccinii-corymbosi (Mvc), is one of the most economically important diseases of blueberries in North America. Mvc is capable of inducing two separate blighting stages during its life cycle. Infected fruits are rendered mummified and unmarketable. Genomic data for this pathogen is lacking, but could be useful in understanding the reproductive biology of Mvc and the mechanisms it deploys to facilitate host infection. In this study, PacBio sequencing and Hi-C interaction data were utilized to create a chromosome-scale reference genome for Mvc. The genome comprises nine chromosomes with a total length of 30 Mb, an N50 length of 4.06 Mb, and an average 413X sequence coverage. A total of 9399 gene models were predicted and annotated, and BUSCO analysis revealed that 98% of 1,438 searched conserved eukaryotic genes were present in the predicted gene set. Potential effectors were identified, and the mating-type (MAT) locus was characterized. Biotrophic effectors allow the pathogen to avoid recognition by the host plant and evade or mitigate host defense responses during the early stages of fruit infection. Following locule colonization, necrotizing effectors promote the mummification of host tissues. Potential biotrophic effectors utilized by Mvc include chorismate mutase for reducing host salicylate and necrotrophic effectors include necrosis-inducing proteins and hydrolytic enzymes for macerating host tissue. The MAT locus sequences indicate the potential for homothallism in the reference genome, but a deletion allele of the MAT locus, characterized in a second isolate, indicates heterothallism. Further research is needed to verify the roles of individual effectors in virulence and to determine the role of the MAT locus in outcrossing and population genotypic diversity.
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http://dx.doi.org/10.1093/g3journal/jkaa052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022979PMC
February 2021

Autonomous Robot for Removing Superficial Traumatic Blood.

IEEE J Transl Eng Health Med 2021 2;9:2600109. Epub 2021 Feb 2.

Department of NeurosurgeryXuanwu HospitalCapital Medical UniversityBeijing100053China.

: To remove blood from an incision and find the incision spot is a key task during surgery, or else over discharge of blood will endanger a patient's life. However, the repetitive manual blood removal involves plenty of workload contributing fatigue of surgeons. Thus, it is valuable to design a robotic system which can automatically remove blood on the incision surface. : In this paper, we design a robotic system to fulfill the surgical task of the blood removal. The system consists of a pair of dual cameras, a 6-DoF robotic arm, an aspirator whose handle is fixed to a robotic arm, and a pump connected to the aspirator. Further, a path-planning algorithm is designed to generate a path, which the aspirator tip should follow to remove blood. : In a group of simulating bleeding experiments on ex vivo porcine tissue, the contour of the blood region is detected, and the reconstructed spatial coordinates of the detected blood contour is obtained afterward. The BRR robot cleans thoroughly the blood running out the incision. : This study contributes the first result on designing an autonomous blood removal medical robot. The skill of the surgical blood removal operation, which is manually operated by surgeons nowadays, is alternatively grasped by the proposed BRR medical robot.
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http://dx.doi.org/10.1109/JTEHM.2021.3056618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880304PMC
February 2021

Role of traditional CHO PET parameters in distinguishing IDH, TERT and MGMT alterations in primary diffuse gliomas.

Ann Nucl Med 2021 Apr 2;35(4):493-503. Epub 2021 Feb 2.

Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, China.

Objective: Isocitrate dehydrogenase (IDH) mutation, telomerase reverse transcriptase (TERT) promoter mutation and O-methylguanine-DNA methyltransferase (MGMT) promoter methylation status are diagnostic, prognostic, predictive and therapeutic biomarkers for primary diffuse gliomas, and this study aimed to explore the relationship between choline (CHO) positron emission tomography (PET) parameters and these molecular alterations.

Methods: Twenty-eight patients who were histopathologically diagnosed with primary diffuse glioma and underwent presurgical CHO PET/CT were retrospectively analyzed, and IDH, TERT and MGMT alterations were examined. The volume of interest (VOI) was semiautomatically defined based on standardized uptake value (SUV) thresholds, and 5 traditional CHO parameters, namely, SUVmax, SUVmean, metabolic tumor volume (MTV), total lesion CHO uptake (TLC) and tumor-to-normal contralateral cortex activity ratio (T/N ratio), were calculated. Wilcoxon rank-sum tests and receiver operating characteristic (ROC) curves were applied to evaluate the differences and performances of the CHO parameters, and their capability to stratify patient prognosis was also evaluated.

Results: All 5 parameters were significantly higher in IDH-wildtype gliomas than in IDH-mutant gliomas (p = 0.0001-0.037), and SUVmax, SUVmean, TLC and the T/N ratio exhibited good performances in distinguishing the IDH status (areas under the ROC curve (AUCs) 0.856-0.918, accuracies 0.857-0.893) as well as stratifying patient prognosis. Although the differences and performances of the traditional parameters in distinguishing diverse TERT and MGMT statuses were moderate in the whole population, the T/N ratio and TLC displayed certain predictive value in discriminating the TERT status in the IDH-mutant and IDH-wildtype subgroups (p = 0.028-0.048, AUCs 0.857-0.860, accuracies 0.800-0.917, respectively).

Conclusions: Traditional CHO PET parameters are capable of distinguishing IDH but not TERT or MGMT alterations in the whole population. In accordance with the clinical understanding of TERT promoter mutations, the T/N ratio and TLC can also discriminate the TERT status in IDH subgroups.
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http://dx.doi.org/10.1007/s12149-021-01589-5DOI Listing
April 2021

miR-146a-3p suppressed the differentiation of hAMSCs into Schwann cells via inhibiting the expression of ERBB2.

Cell Tissue Res 2021 Apr 15;384(1):99-112. Epub 2021 Jan 15.

Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical College, 149 Dalian Road, Guizhou, Zunyi, China.

Human amniotic mesenchymal stem cells (hAMSCs) can be differentiated into Schwann-cell-like cells (SCLCs) in vitro. However, the underlying mechanism of cell differentiation remains unclear. In this study, we explored the phenotype and multipotency of hAMSCs, which were differentiated into SCLCs, and the expression of nerve repair-related Schwann markers, such as S100 calcium binding protein B (S-100), TNF receptor superfamily member 1B (P75), and glial fibrillary acidic protein (GFAP) were observed to be significantly increased. The secreted functional neurotrophic factors, like brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin-3 (NT-3), were determined and also increased with the differentiation time. Moreover, miR-146a-3p, which significantly decreased during the differentiation of hAMSCs into SCLCs, was selected by miRNA-sequence analysis. Further molecular mechanism studies showed that Erb-B2 receptor tyrosine kinase 2 (ERBB2) was an effective target of miR-146a-3p and that miR-146a-3p down-regulated ERBB2 expression by binding to the 3'-UTR of ERBB2. The expression of miR-146a-3p markedly decreased, while the mRNA levels of ERBB2 increased with the differentiation time. The results showed that down-regulating miR-146a-3p could promote SC lineage differentiation and suggested that miR-146a-3p negatively regulated the Schwann-like phenotype differentiation of hAMSCs by targeting ERBB2. The results will be helpful to establish a deeper understanding of the underlying mechanisms and find novel strategies for cell therapy.
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http://dx.doi.org/10.1007/s00441-020-03320-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016804PMC
April 2021

Improving prognostic performance in resectable pancreatic ductal adenocarcinoma using radiomics and deep learning features fusion in CT images.

Sci Rep 2021 Jan 14;11(1):1378. Epub 2021 Jan 14.

Department of Medical Imaging, University of Toronto, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.

As an analytic pipeline for quantitative imaging feature extraction and analysis, radiomics has grown rapidly in the past decade. On the other hand, recent advances in deep learning and transfer learning have shown significant potential in the quantitative medical imaging field, raising the research question of whether deep transfer learning features have predictive information in addition to radiomics features. In this study, using CT images from Pancreatic Ductal Adenocarcinoma (PDAC) patients recruited in two independent hospitals, we discovered most transfer learning features have weak linear relationships with radiomics features, suggesting a potential complementary relationship between these two feature sets. We also tested the prognostic performance for overall survival using four feature fusion and reduction methods for combining radiomics and transfer learning features and compared the results with our proposed risk score-based feature fusion method. It was shown that the risk score-based feature fusion method significantly improves the prognosis performance for predicting overall survival in PDAC patients compared to other traditional feature reduction methods used in previous radiomics studies (40% increase in area under ROC curve (AUC) yielding AUC of 0.84).
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http://dx.doi.org/10.1038/s41598-021-80998-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809062PMC
January 2021

A Routing Algorithm Based on Real-Time Information Traffic in Sparse Environment for VANETs.

Sensors (Basel) 2020 Dec 8;20(24). Epub 2020 Dec 8.

CAS Engineering Laboratory for Intelligent Agricultural Machinery Equipment, Beijing 100000, China.

Because of the specific characteristics, like high vehicular mobility, unstable topology, and interruption of inter-vehicle wireless communication, it is hard to make a perfect decision on packet forwarding in highly dynamic topology VANETs. Especially in a sparse urban environment, the poor connectivity of nodes will mostly cause problems such as data packet loss and routing redundancy. Therefore, how to choose the best relay node becomes a key challenge in the design of fast and reliable routing protocols. This paper presents real-time effective information traffic routing (RTEIT), which provides an optimal route for forwarding the data packets toward their destination when choosing the relay node. RTEIT introduces a new network parameter named effective information traffic which can estimate the connectivity of nodes by the path that has been successfully created. Moreover, for avoiding unexpected communication interruption, we propose a new formula to evaluate the status of the links via considering the speed, direction, and location information. Finally, the node utility, as the criterion of routing decision, is calculated by effective information traffic and link status. We use the simulator of SUMO and NS-3 platform to evaluate RTEIT, and the results are compared with GPSR MOPR, and MM-GPSR. The evaluation results demonstrate that RTEIT outperforms in terms of packet loss rate, end-to-end delay, and network yield.
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http://dx.doi.org/10.3390/s20247018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763438PMC
December 2020

Informal Status and Taking Charge: The Different Roles of OBSE, P-J Fit, and P-S Fit.

Front Psychol 2020 14;11:1994. Epub 2020 Aug 14.

School of Management, Xi'an Jiaotong University, Xi'an, China.

Status in an organization is considered a significant antecedent to an employee's work-related behaviors. However, the relationship between knowledge workers' informal status and "taking charge" has been ignored in previous human resource management research. Based on the self-consistency theory, this study examines the mechanisms underlying the influence of knowledge workers' informal status on taking charge. Data were collected from 337 dyads of employees and their immediate supervisors in 24 enterprises and companies. The results of moderated-mediation analysis indicate organization-based self-esteem (OBSE) fully mediated the positive relationship between knowledge workers' informal status and taking charge, whereas person-job fit (P-J fit) and person-supervisor fit (P-S fit) each moderated the relationship between knowledge workers' informal status and OBSE, in addition to the indirect effect of knowledge workers' informal status on taking charge. Specifically, the indirect effect was strongest when P-J fit or P-S fit was high. The theoretical and managerial implications of the findings, limitations of the study, and future research directions are discussed.
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http://dx.doi.org/10.3389/fpsyg.2020.01994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457060PMC
August 2020

Efficacy of FeO@polydopamine nanoparticle-labeled human umbilical cord Wharton's jelly-derived mesenchymal stem cells in the treatment of streptozotocin-induced diabetes in rats.

Biomater Sci 2020 Oct 1;8(19):5362-5375. Epub 2020 Sep 1.

Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

Diabetes mellitus (DM) is characterized by the irreversible destruction of insulin-secreting pancreatic β-islet cells and requires life-long exogenous insulin therapy. Umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) have been shown to improve islet function in animal models of diabetes. However, inadequate MSC homing to injured sites has limited their efficacy. Since efficient cell therapy heavily relies on appropriate homing to target tissues, increasing the specificity to the target organ and the extent of homing of the injected WJ-MSCs is paramount to successful clinical outcomes. Therefore, in this study, we synthesized FeO@polydopamine nanoparticle (NP)-labeled MSCs and evaluated their therapeutic efficacy in a clinically relevant rat model of streptozotocin-induced diabetes using an external magnetic field. We found that NPs were successfully incorporated into WJ-MSCs and did not negatively affect stem cell properties. Magnetic targeting of WJ-MSCs contributed to long-term cell retention in pancreatic tissue and improved the islet function of diabetic rats, compared to injection of WJ-MSC alone. In addition, anti-inflammatory effects and the anti-apoptotic capacity of WJ-MSCs appeared to play a major role in the functional and structural recovery of the pancreas. Thus, therapy relying on the magnetic targeting of WJ-MSCs may serve as an effective approach for DM treatment.
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http://dx.doi.org/10.1039/d0bm01076fDOI Listing
October 2020

Genomic and transcriptomic insights into Raffaelea lauricola pathogenesis.

BMC Genomics 2020 Aug 20;21(1):570. Epub 2020 Aug 20.

Department of Plant Pathology, University of Florida, 1453 Fifield Hall, Gainesville, FL, 32611-0680, USA.

Background: Laurel wilt caused by Raffaelea lauricola is a lethal vascular disease of North American members of the Lauraceae plant family. This fungus and its primary ambrosia beetle vector Xyleborus glabratus originated from Asia; however, there is no report of laurel wilt causing widespread mortality on native Lauraceae trees in Asia. To gain insight into why R. lauricola is a tree-killing plant pathogen in North America, we generated and compared high quality draft genome assemblies of R. lauricola and its closely related non-pathogenic species R. aguacate.

Results: Relative to R. aguacate, the R. lauricola genome uniquely encodes several small-secreted proteins that are associated with virulence in other pathogens and is enriched in secondary metabolite biosynthetic clusters, particularly polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and PKS-NRPS anchored gene clusters. The two species also exhibit significant differences in secreted proteins including CAZymes that are associated with polysaccharide binding including the chitin binding CBM50 (LysM) domain. Transcriptomic comparisons of inoculated redbay trees and in vitro-grown fungal cultures further revealed a number of secreted protein genes, secondary metabolite clusters and alternative sulfur uptake and assimilation pathways that are coordinately up-regulated during infection.

Conclusions: Through these comparative analyses we have identified potential adaptations of R. lauricola that may enable it to colonize and cause disease on susceptible hosts. How these adaptations have interacted with co-evolved hosts in Asia, where little to no disease occurs, and non-co-evolved hosts in North America, where lethal wilt occurs, requires additional functional analysis of genes and pathways.
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http://dx.doi.org/10.1186/s12864-020-06988-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441637PMC
August 2020

Removal, seasonal variation, and environmental impact of parabens in a municipal wastewater treatment facility in Guangzhou, China.

Environ Sci Pollut Res Int 2020 Aug 13;27(22):28006-28015. Epub 2020 May 13.

School of Civil Engineering, Guangzhou University, Guangzhou, 510006, China.

The occurrence, seasonal variation, and environmental impact of five widely used parabens, methyl-(MeP), ethyl-(EtP), n-propyl-(n-PrP), n-butyl-(n-BuP), and benzyl-(BzP) parabens, were investigated in a municipal wastewater treatment plant (WWTP) located in Guangzhou, China, for 1 year. The concentrations of ∑parabens in the influent and the effluent were 94.2-957 and 0.89-14.7 ng L, respectively. The influent paraben concentrations in autumn were significantly lower than in winter, spring, and summer, and the concentrations were generally higher in spring. The removal efficiencies of ∑parabens in the dissolved phase were over 96%, with high efficiencies in MeP, EtP, and n-PrP. Risk assessment indicated that parabens in the effluent were not likely to pose an environmental risk to aquatic ecosystems. The present study indicates that the treatment processes employed in full-scale WWTPs are effective at removing parabens and highlights the possibility of utilizing WWTPs for restoring water quality in riverine and coastal regions heavily impacted by paraben contamination.
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http://dx.doi.org/10.1007/s11356-020-09083-7DOI Listing
August 2020

[Natural Latex Rubber Condom Inspection and Exploratory Research Analysis].

Zhongguo Yi Liao Qi Xie Za Zhi 2020 Feb;44(2):163-165

Guizhou Medical Device Testing Center, Guiyang, 550004.

According to the situation of 34 batches of natural rubber latex condoms tested by our center in 2018, the unqualified items in the product inspection items and the exploratory research results of D4 and D5 content in silicone oil lubricants are analyzed. We make recommendations on product technical requirements or standards to strengthen the quality supervision of products.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2020.02.014DOI Listing
February 2020

Functionalization of Metal Surface via Thiol-Ene Click Chemistry: Synthesis, Adsorption Behavior, and Postfunctionalization of a Catechol- and Allyl-Containing Copolymer.

ACS Omega 2020 Apr 26;5(13):7488-7496. Epub 2020 Mar 26.

Institute for Materials Chemistry and Engineering, Kyushu University, Nishi-ku, Fukuoka 819-0395, Japan.

Surface functionalization tailors the interfacial properties without impacts on the mechanical strength, which is beneficial for industry and daily applications of various metallic materials. Herein, a two-step surface functionalization strategy, (1) catechol-mediated immobilization of clickable agent and (2) postfunctionalization based on thiol-ene click reaction, is achieved using a copolymer, namely poly[2-(methacryloyloxy)ethylundec-10-enoate]--(-(3,4-dihydroxyphenethyl) methacrylamide) [P(MEUE--DPMAm)]. To reduce the potential side reactions of allylic double bonds in allyl methacrylate during the polymerization, the MEUE are designed and synthesized with better control over the polymer chain growth. The surface functionalization via the two-step method is demonstrated using various thiols, e.g., hydrophobic, hydrophilic, and polymeric thiols under room conditions. Additionally, the hydrophobic-thiol-functionalized anodic aluminum oxide is found to be a candidate for the oil/water separation with a separation efficiency of ∼99.2%. This surface modifier provides practical insights into the further design of functional materials.
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http://dx.doi.org/10.1021/acsomega.0c00259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144137PMC
April 2020

Functional Genomics Insights Into the Pathogenicity, Habitat Fitness, and Mechanisms Modifying Plant Development of sp. PBTS1 and PBTS2.

Front Microbiol 2020 30;11:14. Epub 2020 Jan 30.

Entomology, Plant Pathology, and Weed Science, New Mexico State University, Las Cruces, NM, United States.

Pistachio Bushy Top Syndrome (PBTS) is a recently emerged disease that has strongly impacted the pistachio industry in California, Arizona, and New Mexico. The disease is caused by two bacteria, designated PBTS1 that is related to and PBTS2 that belongs to the species . Here, we assessed the pathogenic character of the causative agents and examined their chromosomal sequences to predict the presence of particular functions that might contribute to the observed co-occurrence and their effect on plant hosts. In diverse assays, we confirmed the pathogenicity of the strains on "UCB-1" pistachio rootstock and showed that they can also impact the development of tobacco species, but concurrently inconsistencies in the ability to induce symptoms were revealed. We additionally evidence that genes are present only in a subpopulation of pure PBTS1 and PBTS2 cultures after growth on synthetic media, that these genes are easily lost upon cultivation in rich media, and that they are enriched for in an environment. Analysis of the chromosomal sequences indicated that PBTS1 and PBTS2 might have complementary activities that would support niche partitioning. Growth experiments showed that the nutrient utilization pattern of both PBTS bacteria was not identical, thus avoiding co-inhabitant competition. PBTS2 appeared to have the potential to positively affect the habitat fitness of PBTS1 by improving its resistance against increased concentrations of copper and penicillins. Finally, mining the chromosomes of PBTS1 and PBTS2 suggested that the bacteria could produce cytokinins, auxins, and plant growth-stimulating volatiles and that PBTS2 might interfere with ethylene levels, in support of their impact on plant development. Subsequent experimentation supported these predictions. Altogether, our data provide an explanation for the observed pathogenic behavior and unveil part of the strategies used by PBTS1 and PBTS2 to interact with plants.
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http://dx.doi.org/10.3389/fmicb.2020.00014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002392PMC
January 2020

CNN-based survival model for pancreatic ductal adenocarcinoma in medical imaging.

BMC Med Imaging 2020 02 3;20(1):11. Epub 2020 Feb 3.

Institute of Medical Science, University of Toronto, Toronto, ON, Canada.

Background: Cox proportional hazard model (CPH) is commonly used in clinical research for survival analysis. In quantitative medical imaging (radiomics) studies, CPH plays an important role in feature reduction and modeling. However, the underlying linear assumption of CPH model limits the prognostic performance. In this work, using transfer learning, a convolutional neural network (CNN) based survival model was built and tested on preoperative CT images of resectable Pancreatic Ductal Adenocarcinoma (PDAC) patients.

Results: The proposed CNN-based survival model outperformed the traditional CPH-based radiomics approach in terms of concordance index and index of prediction accuracy, providing a better fit for patients' survival patterns.

Conclusions: The proposed CNN-based survival model outperforms CPH-based radiomics pipeline in PDAC prognosis. This approach offers a better fit for survival patterns based on CT images and overcomes the limitations of conventional survival models.
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http://dx.doi.org/10.1186/s12880-020-0418-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998249PMC
February 2020

Solvent Effects on Degradative Condensation Side Reactions of Fructose in Its Initial Conversion to 5-Hydroxymethylfurfural.

ChemSusChem 2020 Feb 21;13(3):438. Epub 2020 Jan 21.

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, P.R. China.

Invited for this month's cover is the group of Liangfang Zhu and Changwei Hu at Sichuan University. The image shows a general understanding on the solvent-controlled formation of oligomers (the possible precursors of humins) accompanying with formation of small-molecular carboxylic acids as by-products in the initial reaction stage of fructose dehydration. The Full Paper itself is available at 10.1002/cssc.201902309.
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http://dx.doi.org/10.1002/cssc.201903479DOI Listing
February 2020

Effects of Medium Chain Fatty Acids on Intestinal Health of Monogastric Animals.

Curr Protein Pept Sci 2020 ;21(8):777-784

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China

Medium-chain fatty acids (MCFAs) are the main form of Medium Chain Triglycerides (MCTs) utilized by monogastric animals. MCFAs can be directly absorbed and supply rapid energy to promote the renewal and repair of intestinal epithelial cells, maintain the integrity of intestinal mucosal barrier function, and reduce inflammation and stress. In our review, we pay more attention to the role of MCFAs on intestinal microbiota and mucosa immunity to explore MCFA's positive effect. It was found that MCFAs and their esterified forms can decrease pathogens while increasing probiotics. In addition, being recognized via specific receptors, MCFAs are capable of alleviating inflammation to a certain extent by regulating inflammation and immune-related pathways. MCFAs may also have a certain value to relieve intestinal allergy and inflammatory bowel disease (IBD). Unknown mechanism of various MCFA characteristics still causes dilemmas in the application, thus MCFAs are used generally in limited dosages and combined with short-chain organic acids (SOAs) to attain ideal results. We hope that further studies will provide guidance for the practical use of MCFAs in animal feed.
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http://dx.doi.org/10.2174/1389203721666191231145901DOI Listing
January 2020

Optimized self-interference cancellation based on optical dual-parallel MZM for co-frequency and co-time full duplex wireless communication under nonlinear distortion and emulated multipath effect.

Opt Express 2019 Dec;27(26):37286-37297

Co-frequency and co-time full duplex (CCFD) technique has the potential to further improve the capacity of the fifth-generation (5G) system. However, in a CCFD-based transceiver, the self-interference-cancellation (SIC) module is essential because the transmitted signal is also captured by the receiving antenna. In this paper, an optimized SIC technique is proposed based on a dual-parallel Mach-Zehnder modulator (DP-MZM). In addition to the multipath effect between the transmitting and receiving antennas, the nonlinear distortion induced by the used optical and electrical components is considered and analyzed for the first time. In this scheme, one of the children MZMs is biased at 90°. The bias points of the other child MZM and the parent MZM are swept for SIC optimization. Compared to the traditional SIC scheme using DP-MZM, the power of the received signal is increased by 6 dB and the robustness to the bias point drift is improved. In our experiment, over 35 dB cancellation ratio is achieved for 200 MHz filtered orthogonal frequency division multiplexing (F-OFDM) interference signal with carrier frequency from 2.5 GHz to 6 GHz. To mitigate the influence of multipath effect and nonlinear distortion, the recursive least squared (RLS) linear equalizer and the RLS Volterra equalizer are designed and compared. Finally, 42 dB cancellation ratio is achieved for 200 MHz F-OFDM signal with carrier frequency of 4.5 GHz in the presence of nonlinear distortion and emulated multipath effect.
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http://dx.doi.org/10.1364/OE.27.037286DOI Listing
December 2019

Modeling (in)congruence between dependent variables: The directional and nondirectional difference (DNDD) framework.

J Appl Psychol 2020 Sep 19;105(9):1013-1035. Epub 2019 Dec 19.

This article proposes a new approach to modeling the antecedents of incongruence between 2 dependent variables. In this approach, incongruence is decomposed into 2 orthogonal components representing directional and nondirectional difference (DNDD). Nondirectional difference is further divided into components representing shared and unique variability. We review previous approaches to modeling antecedents of difference, including the use of arithmetic, absolute, and squared differences, as well as the approaches of Edwards (1995) and Cheung (2009). Based on 2 studies, we demonstrate the advantages of DNDD approach compared with other methods. In the first study, we use a Monte Carlo simulation to demonstrate the circumstances under which each type of difference arises, and we compare the insights revealed by each approach. In the second study, we provide an illustrative example of DNDD approach using a field dataset. In the discussion, we review the strengths and limitations of our approach and propose several practical applications. Our article proposes 2 extensions to the basic DNDD approach, including modeling difference with a known target or "true" value, and using multilevel analysis to model nondirectional difference with exchangeable ratings. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
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http://dx.doi.org/10.1037/apl0000475DOI Listing
September 2020

Microwave photonic RF front-end for co-frequency co-time full duplex 5G communication with integrated RF signal self-interference cancellation, optoelectronic oscillator and frequency down-conversion.

Opt Express 2019 Oct;27(22):32147-32157

A novel RF front-end, which could simultaneously realize wideband RF signal self-interference cancellation (SIC), local oscillator (LO) generator based on optoelectronic oscillator (OEO) and frequency down-conversion has been proposed and experimentally demonstrated. In our microwave photonic RF front-end, only one single-polarization optical in-phase and quadrature-phase (IQ) modulator are required. The upper Mach-Zehnder modulator (MZM) of this optical IQ modulator works as a mixer; the lower MZM works as a reference arm; the parent Mach-Zehnder interferometer (MZI) is used to combine two output optical signals of these two child MZMs. In this way, not only self-interference signal is cancelled in optical domain but also frequency down-conversion is realized at the same time. On the other hand, the upper MZM is also shared to form an OEO by using a self-polarization-stabilization technique. By this means, no external LO signal for frequency down-conversion and electrical attenuator for SIC are needed in our scheme, contributing to compact structure and cost reduction. In our proof-of-concept experiment, a LO signal with central frequency of 10 GHz and phase noise of -108.66 dBc/Hz@10kHz is generated. By optimizing the bias points of the used optical IQ modulator, a 5×20MHz 64-ary quadrature amplitude modulation-orthogonal frequency division multiplexing (64QAM-OFDM) LTE-A signal with central frequency of 12.6 GHz is down-converted to 2.6 GHz, and about 28 dB cancellation ratio is achieved. The proposed scheme is suitable for wideband, integrated co-frequency co-time full duplex 5G communication.
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http://dx.doi.org/10.1364/OE.27.032147DOI Listing
October 2019

Solvent Effects on Degradative Condensation Side Reactions of Fructose in Its Initial Conversion to 5-Hydroxymethylfurfural.

ChemSusChem 2020 Feb 22;13(3):501-512. Epub 2019 Oct 22.

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, P.R. China.

The degradative condensation of hexose, which originates from the C-C cleavage of hexose and condensation of degraded hexose fragment, is one of the possible reaction pathways for the formation of humins in hexose dehydration to 5-hydroxymethylfurfural (HMF). Herein, the impacts of several polar aprotic solvents on the degradative condensation of fructose to small-molecule carboxylic acids and oligomers (possible precursors of humins) are reported. In particular, a close relationship between the tautomeric distribution of fructose in solvents and the mechanism of degradative condensation is demonstrated. Typically, α-fructofuranose in 1,4-dioxane and acyclic open-chain fructose in THF favor the conversion of fructose to formic acid and oligomers; α-fructopyranose in γ-valerolactone or N-methylpyrrolidone favors levulinic acid and oligomers, whereas β-fructopyranose in 4-methyl-2-pentanone favors acetic acid and corresponding oligomers. This close correlation highlights a general understanding of the solvent-controlled formation of oligomers, which represents an important step toward the rational design of effective solvent systems for HMF production.
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http://dx.doi.org/10.1002/cssc.201902309DOI Listing
February 2020

The modification of ferroptosis and abnormal lipometabolism through overexpression and knockdown of potential prognostic biomarker perilipin2 in gastric carcinoma.

Gastric Cancer 2020 03 13;23(2):241-259. Epub 2019 Sep 13.

Department of Laboratory Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.

Background: To investigate the biological relationship, mechanism between perilipin2 and the occurrence, advancement of gastric carcinoma, and explore the mechanism of lipid metabolism disorder leading to gastric neoplasm, and propose that perilipin2 is presumably considered as a potential molecular biomarker of gastric carcinoma.

Methods: RNA-seq was applied to analyze perilipin2 and differentially expressed genes modulated by perilipin2 in neoplastic tissues of both perilipin2 overexpression and knockdown groups in vivo. The mechanism was discovered and confirmed by Rt-qPCR, immunoblotting, immunohistochemistry, staining and microassay, respectively. Cellular function experiments were performed by flow cytometry, CCK8, clonogenic assay, etc. RESULTS: Overexpression and knockdown of perilipin2 augmented the proliferation and apoptosis of gastric carcinoma cell lines SGC7901 and MGC803, respectively. The neoplastic cells with perilipin2-overexpression obtained more conspicuously rapid growth than knockdown group in vivo, and perilipin2 affected the proliferation and apoptosis of gastric carcinoma cells by modulating the related genes:acyl-coa synthetase long-chain family member 3, arachidonate 15-lipoxygenase, microtubule associated protein 1 light chain 3 alpha, pr/set domain 11 and importin 7 that were participated in Ferroptosis pathway. Moreover, RNA-seq indicated perilipin2 was an indispensable gene and protein in the suppression of Ferroptosis caused by abnormal lipometabolism in gastric carcinoma.

Conclusion: Our study expounded the facilitation of perilipin2 in regulating the proliferation and apoptosis of gastric carcinoma cells by modification in Ferroptosis pathway, and we interpreted that the mechanism of gastric neoplasm caused by obesity, we also discovered that pr/set domain 11 and importin 7 are novel transcription factors relevant to gastric carcinoma. Furthermore, perilipin2 probably serves not only as a diagnostic biomarker, but also a new therapeutic target.
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http://dx.doi.org/10.1007/s10120-019-01004-zDOI Listing
March 2020

Reducing Unnecessary Prostate Multiparametric Magnetic Resonance Imaging by Using Clinical Parameters to Predict Negative and Indeterminate Findings.

J Urol 2020 02 3;203(2):292-298. Epub 2019 Sep 3.

Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.

Purpose: We sought to develop a triage strategy to reduce negative and indeterminate multiparametric magnetic resonance imaging scans in patients at risk for prostate cancer.

Materials And Methods: In this retrospective study we evaluated 865 patients with no prior prostate cancer diagnosis who underwent prostate multiparametric magnetic resonance imaging between 2009 and 2017. Age, prostate volume, prostate specific antigen and prostate specific antigen density were assessed as predictors of positive multiparametric magnetic resonance imaging, defined as PI-RADS™ (Prostate Imaging Reporting and Data System) version 2/Likert score 4 or greater. The cohort was split into a training cohort of 605 patients and a validation cohort of 260. The optimal threshold to rule out positive multiparametric magnetic resonance imaging was chosen to achieve a negative predictive value greater than 90%.

Results: All clinical variables were significant predictors of positive multiparametric magnetic resonance imaging (p <0.05). Prostate specific antigen density outperformed other parameters in diagnostic accuracy and did not significantly differ compared to a multivariate model (AUC=0.74 vs 0.75). At prostate specific antigen density greater than 0.078 ng/ml sensitivity, specificity, positive and negative predictive values were 94%, 29%, 22% and 95%, respectively, resulting in 25% fewer scans (64 of 260). In the multivariate model sensitivity, specificity, positive and negative predictive values were 85%, 32%, 22% and 91%, respectively, resulting in 29% fewer scans (75 of 260). Biopsies in men who would not have undergone multiparametric magnetic resonance imaging according to our proposed strategies revealed 2 clinically significant prostate cancers using prostate specific antigen density and 1 using the multivariate model.

Conclusions: In patients at risk for prostate cancer applying a multivariate prediction model or a prostate specific antigen density cutoff of 0.078 ng/ml resulted in 25% to 29% fewer multiparametric magnetic resonance imaging scans performed while missing only a minimal number of clinically significant prostate cancers. Further prospective validation is required.
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http://dx.doi.org/10.1097/JU.0000000000000518DOI Listing
February 2020

Homogeneous Freestanding Luminescent Perovskite Organogel with Superior Water Stability.

Adv Mater 2019 Sep 28;31(37):e1902928. Epub 2019 Jul 28.

Department of Materials Science and Engineering, University of California, Los Angeles, CA, 90095, USA.

Metal-halide perovskites have become appealing materials for optoelectronic devices. While the fast advancing stretchable/wearable devices require stability, flexibility and scalability, current perovskites suffer from ambient-environmental instability and incompatible mechanical properties. Recently perovskite-polymer composites have shown improved in-air stability with the protection of polymers. However, their stability remains unsatisfactory in water or high-humidity environment. These methods also suffer from limited processability with low yield (2D film or beads) and high fabrication cost (high temperature, air/moisture-free conditions), thereby limiting their device integration and broader applications. Herein, by combining facile photo-polymerization with room-temperature in-situ perovskite reprecipitation at low energy cost, a one-step scalable method is developed to produce freestanding highly-stable luminescent organogels, within which CH NH PbBr nanoparticles are homogeneously distributed. The perovskite-organogels present a record-high stability at different pH and temperatures, maintaining their high quantum yields for > 110 days immersing in water. This paradigm is universally applicable to broad choices of polymers, hence casting these emerging luminescent materials to a wide range of mechanical properties tunable from rigid to elastic. With intrinsically ultra-stretchable photoluminescent organogels, flexible phosphorous layers were demonstrated with > 950% elongation. Rigid perovskite gels, on the other hand, permitted the deployment of 3D-printing technology to fabricate arbitrary 2D/3D luminescent architectures.
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http://dx.doi.org/10.1002/adma.201902928DOI Listing
September 2019

Effects of different thermal pretreatments on the biodegradability and bioaccessibility of sewage sludge.

Waste Manag 2019 Jul 30;94:68-76. Epub 2019 May 30.

Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region.

Thermal hydrolysis has proven to be a successful approach to make sewage sludge more amenable to anaerobic digestion. Three heat pretreatment scenarios were compared in this study, i.e. thermal alkaline treatment (LAT, 0.1 M NaOH, 80 °C), low temperature thermal treatment (LT, 80 °C) and high temperature thermal treatment (HT, 170 °C). The biodegradability of pretreated sludge was testified by using biochemical methane potential (BMP) test, meanwhile, the repartition and complexities of organic matters in sludge subjected to various pretreatments were characterized by a revised chemical extraction protocol combined with 3D fluorescence spectroscopy. The cumulative methane yield of sewage sludge was significantly increased by LAT (+135%), LT (+95%) and HT (+112%) as compared to the control. Nevertheless, results show that the solubilization degree of sludge was insignificantly correlated to BMP values, meanwhile high correlation values were observed for the soluble polysaccharide concentration in hydrolysate. The degradation rates of bioaccessible fraction of soluble particulate organic matter (SPOM) and readily extractible organic matter (REOM) were improved after thermal pretreatments at varied levels, which indicates that the chemical accessibility is positively correlated with the bioaccessibility. Furthermore, the biodegradable index F was proposed to evaluate the biodegradability of organic matter, which is helpful for the optimization of various pretreatment strategies.
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http://dx.doi.org/10.1016/j.wasman.2019.05.047DOI Listing
July 2019

Amplifier-free 4×96 Gb/s PAM8 transmission enabled by modified Volterra equalizer for short-reach applications using directly modulated lasers.

Opt Express 2019 Jun;27(13):17927-17939

By using four O-band directly modulated lasers (DMLs), for the first time a 384 Gb/s (4 × 96 Gbit/s) 8-level pulse amplitude modulation (PAM8) signal is successfully transmitted over a 15 km standard single mode fiber (SSMF) with no optical amplifier. The nonlinear Volterra equalizer is usually used to cope with the distortions induced by the nonlinearity of DML and the bandwidth-limited components. However, the Volterra equalizer would also enhance the noise at high frequency, which is harmful, especially to PAM8 signal because it is more sensitive to noise. Thus, the Volterra equalizer is modified in our scheme by adding a decision feedback process behind. With the help of the modified Volterra equalizer, the enhanced noise at high frequency is effectively eliminated, and a power gain of 0.5 dB and 3.3 dB for 4 × 64 Gbit/s PAM4 signal transmission over 30 km SSMF and 4 × 96 Gbit/s PAM8 signal transmission over 15 km SSMF at the HD-FEC limit can be obtained, respectively. Moreover, the computation complexity of the modified Volterra equalizer could be reduced by 38% compared with the conventional Volterra equalizer.
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http://dx.doi.org/10.1364/OE.27.017927DOI Listing
June 2019

Work-Family Segmentation Preferences and Work-Family Conflict: Mediating Effect of Work-Related ICT Use at Home and the Multilevel Moderating Effect of Group Segmentation Norms.

Front Psychol 2019 16;10:834. Epub 2019 Apr 16.

School of Public Administration, Southwestern University of Finance and Economics, Chengdu, China.

Drawing on boundary theory, we propose a multilevel model that examines the effects of group segmentation norms on individual-level processes, relating segmentation preferences to work-family conflict via the use of a technological boundary. Data from 350 married employees in 81 working groups were used to test the model. The results of multilevel analysis revealed that work-related information and communication technology (ICT) use at home mediated the relationship between employee work-family segmentation preferences and work-family conflict, and the segmentation norms of the team moderated the relationship between work-family segmentation preferences and work-related ICT use at home. Managerial and practical implications are discussed.
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http://dx.doi.org/10.3389/fpsyg.2019.00834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477997PMC
April 2019

Corrigendum: Prevents Infection in a Mouse Model by Restoring Gut Barrier and Microbiome Regulation.

Front Microbiol 2019 2;10:601. Epub 2019 Apr 2.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Nanfang Hospital, Southern Medical University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fmicb.2018.02976.].
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http://dx.doi.org/10.3389/fmicb.2019.00601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454232PMC
April 2019

A Study of the Mechanism of the Congruence of Leader-Follower Power Distance Orientation on Employees' Task Performance.

Front Psychol 2019 26;10:615. Epub 2019 Mar 26.

Business School, Hubei University, Wuhan, China.

Based on implicit leadership theory, we examine the congruence effect of leader-follower power distance orientation (PDO) on follower trust in supervisor and work engagement, which in turn influences employees' task performance. Results of polynomial regressions on 526 dyads supported the congruence effect hypothesis. The results show that (1) the congruence of leader-follower PDO leads to better performance; (2) under the condition of congruence, subordinate task performance is higher when leader-follower PDO matching in low-low ratings congruence than it is in high-high ratings congruence; (3) under the condition of asymmetrical incongruence, the follower had higher task performance when a leader's PDO is lower than a follower's PDO; (4) trust in supervisor and the work engagement mediate the effect of congruence of leader-follower PDO on employees' task performance; (5) trust in supervisor also mediates the effect of congruence of leader-follower PDO on employees' work engagement.
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http://dx.doi.org/10.3389/fpsyg.2019.00615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448027PMC
March 2019