Publications by authors named "Yuchen Liu"

237 Publications

Preperitoneal herniation as a complication of tansabdominal preperitoneal patch plasty: a report of two cases.

BMC Surg 2021 May 1;21(1):227. Epub 2021 May 1.

Department of Hernia and Abdominal Wall Surgery, Beijing Chaoyang Hospital, Capital Medical University, No. 5 Jingyuan Road, Shijingshan District, Beijing, 100043, China.

Background: Preperitoneal herniation is a rare complication after transabdominal preperitoneal patch plasty (TAPP) and may be caused by inadequate peritoneal closure. We herein report two cases of postoperative small bowel obstruction due to preperitoneal herniation through a disrupted peritoneum.

Case Presentation: Two men in their 70s were admitted to our center because of small bowel obstruction after TAPP. After examinations and unsuccessful conservative treatment, emergency laparoscopic exploration was performed. Preperitoneal herniation through the disrupted peritoneum was found. The herniated small bowel was reduced and the peritoneum was properly reclosed. The patients recovered and were discharged with normal bowel function.

Conclusions: Inadequate peritoneal closure may cause preperitoneal herniation and lead to postoperative small bowel obstruction and even death. Hernia surgeons can avoid this complication by improving their suture technique and paying attention to the procedure details.
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http://dx.doi.org/10.1186/s12893-021-01225-zDOI Listing
May 2021

Sirolimus-based immunosuppression improves the prognosis of liver Transplantation Recipients with low TSC1/2 expression in hepatocellular carcinoma beyond the Milan Criteria.

Eur J Surg Oncol 2021 Apr 19. Epub 2021 Apr 19.

Department of Hepatobiliary and Pancreatic Surgery, The Center for Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China; Zhejiang University Cancer Center, Hangzhou, 310058, China; NHC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou, 310003, China; Institute of Organ Transplantation, Zhejiang University, Hangzhou, 310003, China. Electronic address:

Background: The use of the immunosuppressive agent sirolimus (SRL) following liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) is controversial. Sirolimus is a typical mammalian target of rapamycin (mTOR) inhibitor, and tuberous sclerosis 1-tuberous sclerosis 2 complex (TSC1/TSC2) is an important negative effector in the mTOR pathway. In this study, we investigated the effect of SRL-based immunosuppression on the prognosis of LT recipients with HCC beyond the Milan criteria based on TSC1/2 expression and explored the effect of TSC1 on HCC in vitro and in vivo.

Methods: We retrospectively analyzed 120 HCC patients who underwent LT in our hospital between January 1, 2015 and December 30, 2018. All patients had HCC beyond the Milan criteria and were divided into the SRL group (n = 50) and non-SRL group (n = 70). TSC1/2 expression levels in paraffin-embedded tissues were determined by immunohistochemistry (IHC) and then analyzed as subgroups. Overall survival (OS) and disease-free survival (DFS) were analyzed using the Kaplan-Meier method. TSC1 expression was silenced in Huh-7 and Bel-7402 cell lines for further cell function experiments.

Results: 88.3% of patients were HBV LT recipients. The SRL group exhibited better DFS and OS compared to the non-SRL group (P = 0.02, P = 0.003). Subgroup (TSC1-based or TSC2-based) analyses revealed that patients with low TSC1 or TSC2 expression benefited from sirolimus (DFS: P = 0.046, OS: P = 0.006 for TSC1; DFS: P = 0.05, OS: P = 0.003 for TSC2) compared with patients with high expression. TSC1 knockdown in Huh-7 and Bel-7402 HCC cell lines activated the mTORC1 pathway and enhanced cell proliferation, migration and sensitivity to SRL in vitro and in vivo.

Conclusion: TSC1/2 expression could be used to predict the prognosis of patients with HCC beyond the Milan criteria who underwent SRL-based immunosuppression following LT. TSC1 knockdown promoted HCC malignancy and enhanced sensitivity to SRL.
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http://dx.doi.org/10.1016/j.ejso.2021.04.001DOI Listing
April 2021

Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes.

Front Microbiol 2021 19;12:633276. Epub 2021 Mar 19.

National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.

This study aimed to investigate the effects of dietary yeast culture (YC) supplementation on egg production, egg quality, reproductive performance, immune functions, antioxidant capacity, and intestinal microbial structure of aged hens. A total of 224 Hy-Line Brown layers (54 weeks old) were randomly assigned to two dietary treatments. The control group was fed a basal diet and the YC group was supplemented with YC at 2.0 g/kg of their diet. Each group had seven replicates with 16 hens each. The study was conducted over a period of 8 weeks. Results indicated that YC addition had no significant effect on laying performance. However, it significantly improved egg quality and hatching rate, enhanced ileum crude fat digestibility, increased the serum parameters of lysozyme (LZM) and total antioxidation capacity (T-AOC) ( < 0.05), and reduced serum aspartate aminotransferase (AST) levels ( < 0.05). Using 16S rRNA analysis, we found that addition of YC significantly altered ileum microbial composition. Linear discriminant analysis of effect size (LEfSe) showed significant enrichment of and in the YC group. PICRUSt analysis of the ileal microbiota found that glutathione metabolism, ubiquinone, and other terpenoid-quinone biosynthesis and lipopolysaccharide biosynthesis protein pathways were highly enriched in the YC group compared with the basal diet group. In summary, the addition of YC can improve egg quality, immune functions, antioxidant capacity, reproduction efficiency, and digestive absorption by increasing the abundance of and . Furthermore, it also improves the biosynthesis of lipopolysaccharide proteins, glutathione metabolism, and the synthesis of ubiquinone and other terpenoid-quinone metabolic pathways.
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http://dx.doi.org/10.3389/fmicb.2021.633276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018237PMC
March 2021

Extract of inhibits lipid accumulation and ameliorates HFD-induced obesity in mice through regulating adipose differentiation by decreasing PPARγ and CEBP/α expression.

Food Nutr Res 2021 1;65. Epub 2021 Mar 1.

Tomas Lindahl Nobel Laureate Laboratory, Precision Medicine Research Centre, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, China.

Background: Obesity is a principal risk factor for the development of type 2 diabetes and cardiovascular diseases. Natural plants and/or foods play an important role in the management of obesity. (AAL) is a kind of potherb popular among Asian populations, and it is also consumed as a food ingredient and traditional herbal medicine.

Objective: We investigated the effects of water extract from AAL on high-fat-diet (HFD)-induced obese mice and 3T3-L1 adipocytes to develop a new functional food material.

Design: Nine-week-old male mice were randomly divided into control (chow diet, = 6) and HFD ( = 30) group. From 12-weeks onward, mice in the HFD group were further separated into model (saline, 6 mL/kg), simvastatin (0.11 mg/mL, 6 mL/kg), and AAL treatment (low, middle, and high dosage: 300, 600, and 900 mg/kg) group, with 6 animals per group, while mice in the control group were treated with saline (6 mL/kg). Food intake, body/fat weight, liver/kidney indexes, and lipid profiles were determined. Tissues were fixed with formalin for pathological examination. Western blotting and PCR were performed to evaluate the protein and mRNA expression in 3T3-L1 adipocytes. Oil Red O staining was used to determine lipid accumulation.

Results: AAL administration significantly suppressed body weight gain, and reduced fat pad weight and Lee's index in obese mice, but had no effect on liver/kidney index. AAL also reduced serum cholesterol, triglyceride, and LDL-C and increased HDL-C levels. Histological analysis revealed that AAL significantly ameliorated lipid accumulation in the liver and subcutaneous adipose tissue. , Oil Red O staining showed that AAL inhibited adipose differentiation by down-regulating the gene and protein expression of PPARγ and C/EBPα. AAL also reversed HFD-induced intestinal dysbacteriosis.

Conclusion: AAL water-soluble extract has a significant anti-adipogenic effect in the HFD-induced obese mice model.
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http://dx.doi.org/10.29219/fnr.v65.424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955518PMC
March 2021

Synthesis of RNA-based gene regulatory devices for redirecting cellular signaling events mediated by p53.

Theranostics 2021 4;11(10):4688-4698. Epub 2021 Mar 4.

Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen-Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518000, China.

The gene is a well-known tumor suppressor, and its mutation often contributes to the occurrence and development of tumors. Due to the diversity and complexity of p53 mutations, there is still no effective p53 gene therapy. In this study, we designed and constructed an aptazyme switch that could effectively sense cellular wild-type p53 protein and regulate downstream gene function flexibly. The application of this artificial device in combination with Cre-LoxP and dCas9-VP64 tools achieved a precisely targeted killing effect on tumor cells. The affinity of the aptamer to p53 protein was verified by SPR. p53 aptazyme and gene circuits were chemically synthesized. The function of the gene circuit was detected by cell proliferation assay, apoptosis assay and Western blot. The nude mouse transplantation tumor experiment was used to evaluate the inhibitory effect of gene circuits on tumor cells . The results of the SPR experiment showed that the p53 aptamer RNA sequence had a robust binding effect with p53 protein. The p53 aptazyme could efficiently sense wild-type p53 protein and initiate self-cleavage in cells. The Cre-p53 aptazyme gene circuit and dCas9-VP64/sgRNA mediated gene circuit designed based on p53 aptazyme significantly inhibited the growth and promoted the apoptosis of wild-type p53-deficient cancer cells . In addition, the gene circuits also had a significant inhibitory effect on tumors . The study developed a novel and efficient ribozyme switch for p53-specific recognition and provided a modular strategy for aptazyme binding to cellular proteins. In addition, the p53 aptazyme successfully inhibited tumor growth through a combined application with other synthetic biological tools, providing a new perspective for cancer therapy.
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http://dx.doi.org/10.7150/thno.55856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978309PMC
March 2021

High Expression of CLEC11A Predicts Favorable Prognosis in Acute Myeloid Leukemia.

Front Oncol 2021 2;11:608932. Epub 2021 Mar 2.

Department of Hematology, Chinese People's Liberation Army General Hospital, Beijing, China.

Background: Acute myeloid leukemia (AML) is a heterogeneous disease of the hematopoietic system, for which identification of novel molecular markers is potentially important for clinical prognosis and is an urgent need for treatment optimization.

Methods: We selected C-type lectin domain family 11, member A (CLEC11A) for study several public databases, comparing expression among a variety of tumors and normal samples as well as different organs and tissues. To investigated the relationship between CLEC11A expression and clinical characteristics, we derived an AML cohort from The Cancer Genome Atlas (TCGA); we also investigated the Bloodspot and HemaExplorer databases. The Kaplan-Meier method and log-rank test were used to evaluate the associations between CLEC11A mRNA expression, as well as DNA methylation, and overall survival (OS), event-free survival (EFS), and relapse-free survival (RFS). DNA methylation levels of CLEC11A from our own 28 AML patients were assessed and related to chemotherapeutic outcomes. Bioinformatics analysis of CLEC11A was carried out using public databases.

Results: Multiple public databases revealed that CLEC11A expression was higher in leukemia. The TCGA data revealed that high CLEC11A expression was linked with favorable prognosis (OS -value = 2e-04; EFS -value = 6e-04), which was validated in GSE6891 (OS -value = 0; EFS -value = 0; RFS -value = 2e-03). Methylation of CLEC11A was negatively associated with CLEC11A expression, and high CLEC11A methylation level group was linked to poorer prognosis (OS -value = 1e-02; EFS -value = 2e-02). Meanwhile, CLEC11A hypermethylation was associated with poor induction remission rate and dismal survival. Bioinformatic analysis also showed that CLEC11A was an up-regulated gene in leukemogenesis.

Conclusion: CLEC11A may be used as a prognostic biomarker, and could do benefit for AML patients by providing precise treatment indications, and its unique gene pattern should aid in further understanding the heterogeneous AML mechanisms.
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http://dx.doi.org/10.3389/fonc.2021.608932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966831PMC
March 2021

Titanium Nitride as a New Prospective Material for NanoSQUIDs and Superconducting Nanobridge Electronics.

Nanomaterials (Basel) 2021 Feb 12;11(2). Epub 2021 Feb 12.

Peter Grünberg Institute 5, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.

Nanobridge Josephson junctions and nanometer-scale superconducting quantum interference devices (nanoSQUIDs) based on titanium nitride (TiN) thin films are described. The TiN films have a room temperature resistivity of ~15 µΩ·cm, a superconducting transition temperature of up to 5.3 K and a coherence length (4.2 K) of ~105 nm. They were deposited using pulsed DC magnetron sputtering from a stoichiometric TiN target onto Si (100) substrates that were heated to 800 °C. Electron beam lithography and highly selective reactive ion etching were used to fabricate nanoSQUIDs with 20-nm-wide nanobridge Josephson junctions of variable thickness. X-ray and high-resolution electron microscopy studies were performed. Non-hysteretic () characteristics of the nanobridges and nanoSQUIDs, as well as peak-to-peak modulations of up to 17 µV in the () characteristics of the nanoSQUIDs, were measured at 4.2 K. The technology offers prospects for superconducting electronics based on nanobridge Josephson junctions operating within the framework of the Ginzburg-Landau theory at 4.2 K.
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http://dx.doi.org/10.3390/nano11020466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917904PMC
February 2021

Organoid Cultures Derived From Patients With Papillary Thyroid Cancer.

J Clin Endocrinol Metab 2021 Apr;106(5):1410-1426

Institute of Shenzhen Translational Medicine, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.

Context: Papillary thyroid cancer (PTC) has been one of the most frequent endocrine malignancies around the world. Although most PTC patients have a favorable prognosis, a subgroup of patients die, especially when disease recurrence occurs. There is a pressing need for clinically relevant preclinical thyroid cancer models for personalized therapy because of the lack of in vitro models that faithfully represent the biology of the parental tumors.

Objective: To understand thyroid cancer and translate this knowledge to clinical applications, patient-derived PTC organoids as a promising new preclinical model were established.

Methods: Surgically resected PTC primary tissues were dissociated and processed for organoid derivation. Tumor organoids were subsequently subjected to histological characterization, DNA sequencing, drug screen, and cell proliferation assay, respectively.

Results: We describe a 3-dimensional culture system for the long-term expansion of patient-derived PTC organoid lines. Notably, PTC organoids preserve the histopathological profiles and genomic heterogeneity of the originating tumors. Drug sensitivity assays of PTC organoids demonstrate patient-specific drug responses, and large correlations with the respective mutational profiles. Estradiol was shown to promote cell proliferation of PTC organoids in the presence of estrogen receptor α (ERα), regardless of the expression of ERβ and G protein-coupled ER.

Conclusion: These data suggest that these newly developed PTC-derived organoids may be an excellent preclinical model for studying clinical response to anticancer drugs in a personalized way, as well as provide a potential strategy to develop prevention and treatment options for thyroid cancer with ERα-specific antagonists.
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http://dx.doi.org/10.1210/clinem/dgab020DOI Listing
April 2021

Pancreatic β cells control glucose homeostasis via the secretion of exosomal miR-29 family.

J Extracell Vesicles 2021 Jan 21;10(3):e12055. Epub 2021 Jan 21.

Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) Institute of Artificial Intelligence Biomedicine School of Life Sciences Nanjing University Nanjing Jiangsu China.

Secreted microRNAs (miRNAs) are novel endocrine factors that play essential pathological and physiological roles. Here, we report that pancreatic β cell-released exosomal miR-29 family members (miR-29s) regulate hepatic insulin sensitivity and control glucose homeostasis. Cultured pancreatic islets were shown to secrete miR-29s in response to high levels of free fatty acids (FFAs) in vitro. In vivo, high levels of FFAs, promoted by either high-fat diet (HFD) feeding (physiopathological) or fasting (physiological), increased the secretion of miR-29s into plasma. Intravenous administration of exosomal miR-29s attenuated insulin sensitivity. The overexpression of miR-29s in the β cells of transgenic (TG) mice promoted the secretion of miR-29s and inhibited the insulin-mediated suppression of glucose output in the liver. We used selective overexpression of traceable heterogenous mutant miR-29s in β cells to confirm that islet-derived exosomal miR-29s target insulin signalling in the liver and blunt hepatic insulin sensitivity. Moreover, in vivo disruption of miR-29s expression in β cells reversed HFD-induced insulin resistance. In vitro experiments demonstrated that isolated exosomes enriched in miR-29s inhibited insulin signalling in the liver and increased hepatic glucose production. These results unveil a novel β cell-derived secretory signal-exosomal miR-29s-and provide insight into the roles of miR-29s in manipulating glucose homeostasis.
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http://dx.doi.org/10.1002/jev2.12055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820156PMC
January 2021

Over-expression of RALYL suppresses the progression of ovarian clear cell carcinoma through inhibiting MAPK and CDH1 signaling pathways.

Int J Med Sci 2021 1;18(3):785-791. Epub 2021 Jan 1.

Reproductive Medicine Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

The molecular mechanism in the progression of ovarian clear cell carcinoma (OCCC) remains unclear. This study aimed to investigate the potential function of RAYLY in OCCC. To validate RAYLY expression, immunohistochemistry, quantitative real-time PCR and western blotting were performed in OCCC tissues and the cell lines of OCCC and epithelial ovarian carcinoma (EOC). Subsequently, the biological effects of RALYL were evaluated through colony formation, and cell proliferation, migration and invasion assays. Finally, RNA-sequencing and gene set enrichment analysis (GSEA) were conducted to explore potential mechanism of RALYL in OCCC. In our study, RALYL was significantly down-regulated in a majority of OCCC tissues compared to adjacent non-tumorous tissues, and OCCC cells had a lower expression level of RALYL than that of EOC cells. OCCC patients with high RALYL expression had a better pathological stage and prognosis. , over-expression of RALYL inhibited cell proliferation, migration and invasion in OCCC. GSEA analysis and western blot indicated an enrichment of MAPK and CDH1 signaling pathways in OCCC cells without RALYL over-expression. RALYL played an important role in the progression of OCCC, and might serve as a potential prognostic biomarker and novel therapeutic target for OCCC.
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http://dx.doi.org/10.7150/ijms.51488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797558PMC
January 2021

Blockade of AIM2 inflammasome or α1-AR ameliorates IL-1β release and macrophage-mediated immunosuppression induced by CAR-T treatment.

J Immunother Cancer 2021 Jan;9(1)

Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, Jiangsu, China

Background: Interleukin (IL) 1 released from monocytes/macrophages is one of the critical determinants in mediating the adverse events of chimeric antigen receptor T cell (CAR-T) therapy, including cytokine release syndrome and neurotoxicity. However, the molecular mechanisms of IL-1 production during CAR-T therapy remain unknown.

Methods: The roles of AIM2 and α1-adrenergic receptor (α1-AR) in CAR-T treatment-induced IL-1β release were evaluated by gene silencing, agonist or antagonist treatment. The phenotype switch of macrophages in response to CAR-T treatment was analyzed concerning cytotoxicity of CAR-T cells and proliferation of activated T cells.

Results: This study provided the experimental evidence that CAR-T treatment-induced activation of AIM2 inflammasome of macrophages resulted in the release of bioactive IL-1β. CAR-T treatment-induced α1-AR-mediated adrenergic signaling augmented the priming of AIM2 inflammasome by enhancing IL-1β mRNA and AIM2 expression. Meanwhile, tumor cell DNA release triggered by CAR-T treatment potentiated the activation of AIM2 inflammasome in macrophages. Interestingly, an apparent phenotypic switch in macrophages occurred after interacting with CAR-T/tumor cells, which greatly inhibited the cytotoxicity of CAR-T cells and proliferation of activated T cells through upregulation of programmed cell death-ligand 1 (PD-L1) and indoleamine 2,3-dioxygenase (IDO) in the macrophages. Blockade of AIM2 inflammasome or α1-AR reversed the upregulation of PD-L1 and IDO and the phenotypic switch of the macrophages.

Conclusion: Our study implicates that CAR-T therapy combined with the blockade of AIM2 inflammasome or α1-AR may relieve IL-1β-related toxic side effects of CAR-T therapy and ensure antitumor effects of the treatment.
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http://dx.doi.org/10.1136/jitc-2020-001466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797290PMC
January 2021

Phase I Trial of Fourth-Generation Anti-CD19 Chimeric Antigen Receptor T Cells Against Relapsed or Refractory B Cell Non-Hodgkin Lymphomas.

Front Immunol 2020 27;11:564099. Epub 2020 Nov 27.

Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: The administration of second- or third-generation anti-CD19 chimeric antigen receptor (CAR) T cells has remarkably improved the survival of patients with relapsed or refractory B cell malignancies. However, there are limited clinical results from fourth-generation CAR-T cell therapy, and the factors affecting response rate and survival have not been fully determined.

Methods: Lymphoma patients with progression or relapse after intensive treatments, including hematopoietic stem cell transplantation, and life expectancy >2 months were enrolled in the study. Peripheral lymphocytes were collected through apheresis, and magnetically selected T cells were lentivirally transduced with a 4th-generation CAR featuring an anti-CD19 CAR and the iCasp9 suicide switch (4SCAR19). The patients received 4SCAR19 T cell infusion after approximately seven days of expansion and a conditioning regimen comprising cyclophosphamide/fludarabine. The efficacy, safety, and risk factors were evaluated.

Results: A total of 21 patients with relapsed/refractory B cell non-Hodgkin lymphoma were enrolled and received 4SCAR19 T cell infusions at a median dose of 8.9×10 CAR-T cells/kg. The overall response rate was 67% [95% confidence interval (CI), 43 to 85], with 43% of patients achieving a complete response and 24% having a partial response. The overall and complete response rates were 58 and 33% in the diffuse large B-cell lymphoma (DLBCL) group and 78 and 56% in the non-DLBCL group, respectively. The median overall survival was 23.8 months (95% CI, not reached), with a median follow-up of 13.7 months. Factors affecting overall survival were International Prognostic Index (IPI), disease type, and remission status after CAR-T cell treatment. The most common adverse events of grade 3 or 4 during treatment were neutropenia (76%), leukopenia (71%), and thrombocytopenia (29%). The incidence of cytokine release syndrome (CRS) was 14%, and all cases were grade 1. One patient developed grade 3 neurotoxicity. No deaths were attributed to infusion of 4SCAR19 T cells, CRS, or neurotoxicity.

Conclusions: In this study, patients with relapsed or refractory B cell non-Hodgkin's lymphoma who received 4SCAR19 T cell therapy had durable responses and few of adverse events. The IPI model is suitable for evaluating the prognosis of patients receiving CAR-T cell therapy.

Trial Registration: Chinese Clinical Trial Registry (http://www.chictr.org.cn): ChiCTR-OOC-16007779.
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http://dx.doi.org/10.3389/fimmu.2020.564099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731732PMC
May 2021

Characterization of Two New Mutations From an EMS-Mutagenic Maize Population for Lignocellulosic Biomass Utilization.

Front Plant Sci 2020 16;11:594798. Epub 2020 Nov 16.

Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Gene mutations linked to lignin biosynthesis are responsible for the phenotypes. The mutants have a brown-reddish midrib associated with changes in lignin content and composition. Maize is caused by a mutation of the cinnamyl alcohol dehydrogenase gene . Here, we generated two new mutant alleles ( and ) through EMS mutagenesis, which contained a single nucleotide mutation (Zm and Zm). The corresponding proteins, ZmCAD2-1 and ZmCAD2-2 were modified with Cys103Ser and Gly185Asp, which resulted in no enzymatic activity . Sequence alignment showed that CAD proteins have high similarity across plants and that Cys103 and Gly185 are conserved in higher plants. The lack of enzymatic activity when Cys103 was replaced for other amino acids indicates that Cys103 is required for its enzyme activity. Enzymatic activity of proteins encoded by genes in plants is 23-98% lower than in the wild type, which leads to lower lignin content and different lignin composition. The mutants have higher saccharification efficiency in maize and could therefore provide new and promising breeding resources in the future.
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http://dx.doi.org/10.3389/fpls.2020.594798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7703671PMC
November 2020

Exploring the perspective of nano-TiO in hydrophobic modified cationic flocculant preparation: Reaction kinetics and emulsified oil removal performance.

Chemosphere 2021 Jan 23;263:128066. Epub 2020 Aug 23.

College of Civil Engineering, Sichuan Agricultural University, Chengdu, 611830, China; Sichuan Higher Education Engineering Research Center for Disaster Prevention and Mitigation of Village Construction, Department of Municipal Engineering, Chengdu, 611830, China.

To reduce the polymerization difficulty of hydrophobic modified copolymers, a hydrophobic modified cationic flocculant was fabricated using nano-TiO as initiator with acrylamide (AM) and methyl acryloxyethyl dimethyl benzyl ammonium chloride (DML) as monomers, and named it PAD. The copolymers were characterized by scanning electron microscopy (SEM), nuclear magnetic resonance (H NMR), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TG). Results verified that PAD was synthesized successfully and nano-TiO was more conducive to DML grafting than traditional photo-initiators. Reaction kinetics demonstrated that the polymerization process was a typical precipitation polymerization initiated by free radicals. Flocculation performance of flocculant on simulated emulsified oil was evaluated and optimized. The simulation results indicated that the flocculation performance of PAD was superior to traditional flocculant, which was attributed to the higher content of DML in PAD. The maximum removal rate of emulsified oil could reach 92.10%, and the corresponding turbidity removal rate was 93.54%. Further, the mechanism studies suggested that the removal of emulsified oil was realized by the synergistic effects of electric neutralization, demulsification, hydrophobic association and adsorption bridging. The findings of this study showed that nano-TiO exhibited a promising prospect in the field of polymer-initiated polymerization.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128066DOI Listing
January 2021

Mandibular involvement in SAPHO syndrome: a retrospective study.

Orphanet J Rare Dis 2020 11 5;15(1):312. Epub 2020 Nov 5.

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China.

Background: Mandible osteomyelitis can occur in synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome, a rare chronic inflammatory disease; however, few studies have explored its characteristics and management.

Methods: We reviewed the medical records of consecutive SAPHO patients with mandible involvement diagnosed in Peking Union Medical College Hospital from September 2014 to July 2019. Demographic, clinical, laboratory, and imaging data were collected at baseline. Prescription data and follow-up magnetic resonance imaging (MRI) and cone beam computed tomography (CBCT) images were collected from the hospital information system. An electronic questionnaire was distributed to all patients to obtain their latest symptoms.

Results: A total of 26 SAPHO patients with mandibular involvement were involved, all of whom responded to the questionnaire (38.5% male; median age, 28 years; median follow-up duration, 2.1 years). Ten patients (38.5%) had undergone an oral procedure 1 month before the onset of mandibular symptoms. All 14 of the patients who underwent a surgical intervention relapsed within a median duration of 2 months (range 0.25-4.0 months), and 24 patients (92.3%) achieved improvement with conservative treatment. Following bisphosphonate treatment, remission of bone marrow oedema and osteolysis was observed on MRI and CBCT, and 5 patients receiving bisphosphonates with follow-up CBCT after remission did not relapse in 5.4 months (mean 6.0, range 3.2-9.9 months).

Conclusion: Mandibular involvement of SAPHO syndrome predominantly occurs in young women. Dental procedures are a possible risk factor. Conservative treatment, especially intravenous bisphosphonates, can lead to oral improvement.
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http://dx.doi.org/10.1186/s13023-020-01589-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643311PMC
November 2020

Improving transgene expression and CRISPR-Cas9 efficiency with molecular engineering-based molecules.

Clin Transl Med 2020 Oct;10(6):e194

Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.

As a novel and robust gene-editing tool, the Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated protein 9 (CRISPR-Cas9) system has revolutionized gene therapy. Plasmid vector delivery is the most commonly used method for integrating the CRISPR-Cas9 system into cells. However, such foreign cytosolic DNAs trigger an innate immune response (IIR) within cells, which can hinder gene editing by inhibiting transgene expression. Although some small molecules have been shown to avoid the action of IIR on plasmids, they only work on a single target and may also affect cell viability. A genetic approach that works at a comprehensive level for manipulating IIR is still lacking. Here, we designed and constructed several artificial nucleic acid molecules (ANAMs), which are combinations of aptamers binding to two key players of IIR (β-catenin and NF-κB). ANAMs strongly inhibited the IIR in cells, thus improving transgene expression. We also used ANAMs to improve the gene-editing efficiency of the CRISPR-Cas9 system and its derivatives, thus enhancing the apoptosis of cancer cells induced by CRISPR-Cas9. ANAMs can be valuable tools for improving transgene expression and gene editing in mammalian cells.
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http://dx.doi.org/10.1002/ctm2.194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533053PMC
October 2020

Synthesizing AND gate minigene circuits based on CRISPReader for identification of bladder cancer cells.

Nat Commun 2020 10 30;11(1):5486. Epub 2020 Oct 30.

National and Local Joint Engineering Laboratory of Medical Synthetic Biology, Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Health Science Center, 518035, Shenzhen, China.

The logical AND gate gene circuit based on the CRISPR-Cas9 system can distinguish bladder cancer cells from normal bladder epithelial cells. However, the layered artificial gene circuits have the problems of high complexity, difficulty in accurately predicting the behavior, and excessive redundancy, which cannot be applied to clinical translation. Here, we construct minigene circuits based on the CRISPReader, a technology used to control promoter-less gene expression in a robust manner. The minigene circuits significantly induce robust gene expression output in bladder cancer cells, but have nearly undetectable gene expression in normal bladder epithelial cells. The minigene circuits show a higher capability for cancer identification and intervention when compared with traditional gene circuits, and could be used for in vivo cancer gene therapy using the all-in-one AAV vector. This approach expands the design ideas and concepts of gene circuits in medical synthetic biology.
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http://dx.doi.org/10.1038/s41467-020-19314-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599332PMC
October 2020

Comprehensive Exome Analysis of Immunocompetent Metastatic Head and Neck Cancer Models Reveals Patient Relevant Landscapes.

Cancers (Basel) 2020 Oct 12;12(10). Epub 2020 Oct 12.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.

Immunocompetent metastatic head and neck cancer (HNC) models, although scarce, can help understanding cancer progression and therapy responses in vivo. Their comprehensive genome characterizations are essential for translational research. We first exome-sequenced the two most widely used spontaneous metastatic immunocompetent models, namely AT-84 and SCC VII, followed by comprehensive genomic analyses with three prior-sequenced models (MOC2, MOC2-10, and 4MOSC2), together with patient tumors for utility assessment. AT-84 and SCC VII bear high HNC tumor resemblance regarding mutational signatures-, Fanconi anemia, and MAPK and PI3K pathway defects. Collectively, the five models harbor genetic aberrations across 10 cancer hallmarks and 14 signaling pathways and machineries (metabolic, epigenetic, immune evasion), to extents similar in patients. Immune defects in (, , , and ), , , , , , and are identified. Invasion/metastatic genome analyses first highlight potential druggable and mutations, for advanced/metastatic oral cavity cancer, as well as known metastasis players (, , , and ) frequently captured by all models. Notable immunotherapy and precision druggable targets (, , , /, , and ) and three druggable hubs (RTK family, MAPK, and DNA repair pathways) are frequently represented by these models. Immunocompetent metastatic HNC models are worth developing to address therapy- and invasion/metastasis-related questions in host immunity contexts.
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http://dx.doi.org/10.3390/cancers12102935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601118PMC
October 2020

A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection.

EBioMedicine 2020 Nov 9;61:103036. Epub 2020 Oct 9.

Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, China.

Background: Real-time reverse transcription-PCR (rRT-PCR) has been the most effective and widely implemented diagnostic technology since the beginning of the COVID-19 pandemic. However, fuzzy rRT-PCR readouts with high Ct values are frequently encountered, resulting in uncertainty in diagnosis.

Methods: A Specific Enhancer for PCR-amplified Nucleic Acid (SENA) was developed based on the Cas12a trans-cleavage activity, which is specifically triggered by the rRT-PCR amplicons of the SARS-CoV-2 Orf1ab (O) and N fragments. SENA was first characterized to determine its sensitivity and specificity, using a systematic titration experiment with pure SARS-CoV-2 RNA standards, and was then verified in several hospitals, employing a couple of commercial rRT-PCR kits and testing various clinical specimens under different scenarios.

Findings: The ratio (10 min/5 min) of fluorescence change (FC) with mixed SENA reaction (mix-FCratio) was defined for quantitative analysis of target O and N genes, and the Limit of Detection (LoD) of mix-FCratio with 95% confidence interval was 1.2≤1.6≤2.1. Totally, 295 clinical specimens were analyzed, among which 21 uncertain rRT-PCR cases as well as 4 false negative and 2 false positive samples were characterized by SENA and further verified by next-generation sequencing (NGS). The cut-off values for mix-FCratio were determined as 1.145 for positive and 1.068 for negative.

Interpretation: SENA increases both the sensitivity and the specificity of rRT-PCR, solving the uncertainty problem in COVID-19 diagnosis and thus providing a simple and low-cost companion diagnosis for combating the pandemic.

Funding: Detailed funding information is available at the end of the manuscript.
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http://dx.doi.org/10.1016/j.ebiom.2020.103036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544594PMC
November 2020

β2-AR activation promotes cleavage and nuclear translocation of Her2 and metastatic potential of cancer cells.

Cancer Sci 2020 Dec 27;111(12):4417-4428. Epub 2020 Oct 27.

Cancer Institute, Xuzhou Medical University, Xuzhou, China.

Prolonged hypersecretion of catecholamine induced by chronic stress may correlate with malignant progression of cancer. β2-adrenergic receptor (β2-AR) overexpressed in certain cancer cells may translate the signals from neuroendocrine system to malignant signals by interacting with oncoproteins, such as Her2. In the present study, we demonstrate that catecholamine stimulation activates the expression and proteolytic activity of ADAM10 by modulating the expression of miR-199a-5p and SIRT1 and also confirm that catecholamine induction triggers the activities of γ-secretase, leading to shedding of Her2 extracellular domain (ECD) by ADAM10 and subsequent intramembranous cleavage of Her2 intracellular domain (ICD) by presenilin-dependent γ-secretase, nuclear translocation of Her2 ICD, and enhanced transcription of tumor metastasis-associated gene COX-2. Chronic stimulation of catecholamine strongly promotes the invasive activities of cancer cells in vitro and spontaneous tumor lung metastasis in mice. Furthermore, nuclear localization of Her2 was significantly correlated with overexpression of β2-AR in human breast cancer tissues, indicating that catecholamine-induced β2-AR activation plays decisive roles in tumor metastasis. Our data also reveal that an unknown mechanism by which the regulated intramembrane proteolysis (RIP) initiated by β2-AR-mediated signaling controls a novel Her2-mediated signaling transduction.
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http://dx.doi.org/10.1111/cas.14676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734010PMC
December 2020

Gut microbial bile acid metabolite skews macrophage polarization and contributes to high-fat diet-induced colonic inflammation.

Gut Microbes 2020 11;12(1):1-20

Department of Pediatric Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University , Shanghai, China.

High-fat diet (HFD) leads to systemic low-grade inflammation, which has been involved in the pathogenesis of diverse metabolic and inflammatory diseases. Colon is thought to be the first organ suffering from inflammation under HFD conditions due to the pro-inflammatory macrophages infiltration, however, the mechanisms concerning the induction of pro-inflammatory phenotype of colonic macrophages remains unclear. In this study, we show that HFD increased the percentage of gram-positive bacteria, especially genus , and resulted in the significant increment of fecal deoxycholic acid (DCA), a gut microbial metabolite produced by bacteria mainly restricted to genus . Notably, reducing gram-positive bacteria with vancomycin diminished fecal DCA and profoundly alleviated pro-inflammatory macrophage infiltration in colon, whereas DCA-supplemented feedings to vancomycin-treated mice provoked obvious pro-inflammatory macrophage infiltration and colonic inflammation. Meanwhile, intra-peritoneal administration of DCA also elicited considerable recruitment of macrophages with pro-inflammatory phenotype. Mechanistically, DCA dose-dependently promoted M1 macrophage polarization and pro-inflammatory cytokines production at least partially through toll-like receptor 2 (TLR2) transactivated by M2 muscarinic acetylcholine receptor (M2-mAchR)/Src pathway. In addition, M2-mAchR mediated increase of TLR2 transcription was mainly achieved via targeting AP-1 transcription factor. Moreover, NF-κB/ERK/JNK signalings downstream of TLR2 are involved in the DCA-induced macrophage polarization. In conclusion, our findings revealed that high level DCA induced by HFD may serve as an initiator to activate macrophages and drive colonic inflammation, thus offer a mechanistic basis that modulation of gut microbiota or intervening specific bile acid receptor signaling could be potential therapeutic approaches for HFD-related inflammatory diseases.
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http://dx.doi.org/10.1080/19490976.2020.1819155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553752PMC
November 2020

Frequency selective topological edge wave routing in meta-structures made of cylinders.

Opt Lett 2020 Oct;45(19):5608-5611

The propagation direction of edge states is essentially related to the band topology invariant of the constituent structures and the momentum of the excitation source. However, it is difficult to control the propagation path when the chirality of the excitation source and the boundary structures are determined. Here, we study a frequency selective waveguide structure based on photonic crystals with different topological invariant characterized by bulk polarization. By designing different types of interface made from spatially arranged dielectric rods, distinct topological edge states could be realized at different frequencies in the band gap. Therefore, we can construct a meta-structure in which the wave guiding path can be switched by the excitation frequency. Our study provides an alternative approach to designing topological devices such as frequency dependent optical waveguides and frequency division devices.
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http://dx.doi.org/10.1364/OL.405512DOI Listing
October 2020

Hepatic Hippo signaling inhibits development of hepatocellular carcinoma.

Clin Mol Hepatol 2020 10 28;26(4):742-750. Epub 2020 Sep 28.

Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA.

Primary liver cancer is one of the most common cancer worldwide. Hepatocellular carcinoma (HCC) in particular, is the second leading cause of cancer deaths in the world. The Hippo signaling pathway has emerged as a major oncosuppressive pathway that plays critical roles inhibiting hepatocyte proliferation, survival, and HCC formation. A key component of the Hippo pathway is the inhibition of yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ) transcription factors by the Hippo kinase cascade. Aberrant activation of YAP or TAZ has been found in several human cancers including HCC. It is also well established that YAP/TAZ activation in hepatocytes causes HCC in mouse models, indicating that YAP/TAZ are potential therapeutic targets for human liver cancer. In this review, we summarize the recent findings regarding the multifarious roles of Hippo/YAP/TAZ in HCC development, and focus on their cell autonomous roles in controlling hepatocyte proliferation, differentiation, survival and metabolism as well as their non-cell autonomous in shaping the tumor microenvironment.
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http://dx.doi.org/10.3350/cmh.2020.0178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641559PMC
October 2020

Intensity of anticoagulation and survival in patients hospitalized with COVID-19 pneumonia.

Thromb Res 2020 12 23;196:375-378. Epub 2020 Sep 23.

Division of Hematology/Oncology, Warren Alpert Medical School of Brown University, Providence, RI, United States of America.

Background: SARS-CoV-2 infection has noted derangements in coagulation markers along with significant thrombotic complications. Post-mortem examinations show severe endothelial injury and widespread thrombotic microangiopathy in the pulmonary vasculature. Early reports describing the use of prophylactic anticoagulation demonstrated improved survival, leading to the adoption of prophylactic and therapeutic anticoagulation guided by D-dimer levels. The clinical usefulness of D-dimer values, trends, and more intensive anticoagulation remains an area of clinical interest.

Objectives: Assess the outcomes and laboratory trends in COVID-19 patients stratified by intensity of anticoagulation at time of admission.

Patients And Methods: Retrospectively review the differences in clinical outcomes and laboratory trends in patients hospitalized with COVID-19 in the Lifespan Health System.

Results: Between 27 February and 24 April 2020, 468 patients were hospitalized. Initial use of high-intensity thromboprophylaxis was associated with improved 30-day mortality (adjusted RR 0.26; 95% confidence interval [CI], 0.07-0.97; p = 0.045) without a significant increased rate of bleeding (p = 0.11). In severe COVID-19, D-dimer significantly increased during hospitalization with standard thromboprophylaxis (p < 0.001) but remained stable or decreased with high-intensity prophylaxis or therapeutic anticoagulation.

Conclusion: Patients who received high-intensity prophylactic anticoagulation had a downtrend in D-dimer levels and improved 30-day mortality. This suggests a role in anticoagulation in mitigating adverse outcomes associated with COVID-19; however, further randomized, prospective studies are needed.
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http://dx.doi.org/10.1016/j.thromres.2020.09.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511207PMC
December 2020

Transition Metal Dichalcogenide (TMD) Membranes with Ultrasmall Nanosheets for Ultrafast Molecule Separation.

ACS Appl Mater Interfaces 2020 Oct 28;12(40):45453-45459. Epub 2020 Sep 28.

Institute for Frontier Materials, Deakin University, Waurn Ponds Campus, Geelong, Victoria 3220, Australia.

Two-dimensional (2D) transition metal dichalcogenide membranes have entered the spotlight for nanofiltration application owing to the novel mass transport properties in nanochannels. However, further improving the water permeability with high molecular separation rate simultaneously is challenging. In this work, to achieve ultrafast molecule separation, MoS and WS nanosheets with ultrasmall lateral size (<100 nm) are fabricated by sucrose-assisted mechanochemical exfoliation. Ultrasmall nanosheets in the membranes cut down average length of water-transporting paths and create more nanochannels and nanocapillaries for water molecules to pass through membranes. The water flux of these kinds of MoS and WS membranes are significantly enhanced to 918 and 828 L/m h bar, respectively, which is four and two times higher than those of previously reported MoS and WS membranes with larger lateral size nanosheets. In addition, MoS and WS membranes display excellent rejection performance for rhodamine B and Evans blue with a high rejection rate (∼99%). This study provides a promising method to improve the performance of 2D laminar membranes for nanofiltration application by using ultrasmall 2D nanosheets.
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http://dx.doi.org/10.1021/acsami.0c10653DOI Listing
October 2020

Identification of differentially expressed circular RNAs in human nasopharyngeal carcinoma.

Cancer Biomark 2020 ;29(4):483-492

Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, Guangdong, China.

Background: Circular RNAs (circRNAs) are endogenous RNAs that have a covalent closed cycle configuration. circRNAs have been found to be differentially expressed in many human cancers. Therefore, circRNAs may be ideal biomarkers for the diagnosis and treatment of cancer. However, we know very little about the function of circRNAs in nasopharyngeal carcinoma (NPC). The purpose of this study was to evaluate the circRNA expression profiles in NPC.

Methods: We utilized high-throughput RNA sequencing (RNA-Seq) to evaluate the circRNA expression profile in NPC A total of 13,561 unique circRNA candidates were detected. Selection of aberrantly expressed circRNAs was carried out using a q-value of < 0.001 with a fold change of > 2.0 or < 0.5. We carried out Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses to identify the biological functions of differentially expressed circRNAs. Moreover, bioinformatics analyses were implemented to predict the effects between circRNAs and cancer-related microRNAs (miRNAs), and we used Cytoscape to build a cancer-related circRNA-miRNA target gene map. Finally, to verify dysregulated circRNAs, quantitative real-time PCR was utilized.

Results: In NPC tissues, we found that 73 circRNAs were downregulated and 59 were upregulated. The top 12 candidate circRNAs were selected from several vital NPC pathways such as the human papillomavirus and Epstein-Barr virus infection signaling pathways (hsa05165 and hsa05169, respectively), Hepatitis B (hsa05161), and the Ras signaling pathway (hsa04014). A network map of circRNA-miRNA interactions of 12 differentially expressed circRNAs was built. Hsa_circ_0007637 expression distinguished NPC tissues from paired healthy tissues and NPC cell lines (HNE1 6-10B, 5-8F, CNE-2, and so on) from a normal epithelial (NP460) cell line.

Conclusions: In this study, we investigated the profiles of differentially expressed circRNAs in NPC, and our results show that hsa_circ_0007637 may be a biomarker for NPC and play a role in its development. This observation-based research identified dysregulated circRNAs in NPC, which may assist in the development of biomarkers for this disease. Further studies on the mechanisms and functions of these circRNAs may promote our understanding of NPC tumorigenesis.
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http://dx.doi.org/10.3233/CBM-201731DOI Listing
January 2020

A Controllable Inflammatory Response and Temporary Abnormal Coagulation in Moderate Disease of COVID-19 in Wuhan, China.

J Clin Med Res 2020 Sep 15;12(9):590-597. Epub 2020 Aug 15.

Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, China.

Background: The coronavirus disease 2019 (COVID-19) is now a worldwide challenge for public health. Among 7 million patients, about 80% present mild to moderate disease, but studies dedicate to these patients are actually scarce. The aim of our study is to clarify the characteristics of laboratory test index of COVID-19 patient with moderate symptoms during the first wave of the pandemic in Wuhan, China.

Methods: In this retrospective cohort study, we included 107 adult inpatients with confirmed moderate disease of COVID-19 from the Affiliated Hospital of Jianghan University during February and early March 2020. All of these patients were recovered from COVID-19 and discharged from hospital. Demographic, clinical, and laboratory data of admission and discharge were extracted from electronic medical records and analyzed using SPSS, as well as among young, middle age and elderly people.

Results: The median age of this cohort of patients was 56.0 years. And the median hospitalization time was 16 days. Common clinical manifestations included fever, cough, asthenia and shortness of breath. On admission, laboratory results showed normal or increased neutrophil ratio, low lymphocyte count, decreased hemoglobin level, and increased inflammatory indicators (erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP)); and some patients were complicated with coagulation disorder and myocardial damage. Furthermore, patients older than 60 years had statistically higher CRP, ESR and fibrinogen level. As the health condition was improved at discharge, the median level of most laboratory results were in the normal range except hemoglobin and related blood cell count, as well as inflammatory indicator ESR. And patients older than 60 years showed slower recovery on coagulation parameters when compared to younger patients.

Conclusions: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces a controllable inflammatory response in moderate disease of COVID-19 in Wuhan, China. Since patients older than 60 years had higher inflammatory state and more dysregulated coagulation condition, it might be essential to closely assess their illness.
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http://dx.doi.org/10.14740/jocmr4293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430917PMC
September 2020

Analysis of operation procedure and effect for emergency surgery in general hospital during novel coronavirus pneumonia period.

BMC Surg 2020 Aug 26;20(1):190. Epub 2020 Aug 26.

Department of Hernia and Abdominal Wall Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China.

Background: Novel coronavirus pneumonia (NCP) outbreak in Wuhan, China in early 2020, resulted in over 80 thousand infections in China. At present, NCP has an explosive growth in the world. Surgeons could refuse selective operation during the outbreak, but they must face the emergency operation. We hope to avoid the spread of NCP while ensuring efficient treatment of emergency cases.

Methods: The data of patients with incarcerated hernia admitted to Beijing Chaoyang Hospital during NCP epidemic were analyzed and compared with those in 2019. All cases were divided into NCP group and 2019 group. The operation data and inpatient protection process of emergency cases were analyzed. Result During the NCP epidemic, 17 cases with incarcerated hernia were treated in our department. A Total of 263 cases of the same disease were admitted in 2019. There was no significant difference in age, gender, BMI and hernia type between two groups. No significant difference was observed between the two groups in operation method and hospital stay. The waiting time for emergency operation of NCP group was significantly longer than that of 2019 group (P = 0.002). A buffer ward was set up by administrator of hospital during NCP outbreak. Hospitals were divided into "Red area, Yellow area and Green area" artificially, and strict screening consultation system was implemented. There was no case of SARS-nCoV-2 infection in medical staff.

Conclusion: It was safe and effective to carry out emergency operation on the premise of screening, protection and isolation during the NCP epidemic. The increased waiting time for operation due to NCP screening did not threaten medical safety of emergency incarcerated hernia patients.
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http://dx.doi.org/10.1186/s12893-020-00852-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447598PMC
August 2020

Severe deformity in long-term SAPHO syndrome.

Rheumatology (Oxford) 2021 02;60(2):982-983

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

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http://dx.doi.org/10.1093/rheumatology/keaa307DOI Listing
February 2021