Publications by authors named "Yuanyuan Wang"

1,794 Publications

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Metal-based Aerogels catalysts for Electrocatalytic CO2 Reduction.

Chemistry 2022 Aug 17. Epub 2022 Aug 17.

Nanjing University of Posts and Telecommunications, Institute of Advanced Materials, No.9, Wenyuan Road, Yadong New District, 210023, Nanjing, CHINA.

Carbon dioxide, the most widely distributed carbon source in nature, has been widely investigated for its reduction and reuse. In this field, nanometallic materials have been widely studied because of their excellent photo/electrocatalytic activity. As one of the nanometallic materials, metal aerogel has the unique advantages of high electron transfer rate and large specific surface area, as well as rich pore space and strong adsorption performance, which makes it a highly researched material for catalytic CO 2 reduction in recent years. In this paper, we firstly report the latest preparation methods of metal aerogels and the recent progress in electrocatalytic CO 2 reduction. After that, the properties of several typical metallic aerogels and their latest applications in electrocatalytic CO 2 reduction are described. Finally, the vision and research directions for the future industrialization of metal aerogels for electrocatalytic CO 2 reduction are proposed from the perspective of practical applications.
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http://dx.doi.org/10.1002/chem.202201834DOI Listing
August 2022

A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement .

Chem Sci 2022 Aug 27;13(30):8813-8820. Epub 2022 Jun 27.

Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid 28040 Madrid Spain.

The development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments ( subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor π-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both and in the bladders of living rats.
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http://dx.doi.org/10.1039/d2sc02021aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350589PMC
August 2022

Association between popliteal artery wall thickness and structural progression in patients with symptomatic knee osteoarthritis.

Rheumatology (Oxford) 2022 Aug 16. Epub 2022 Aug 16.

School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC, 3004, Australia.

Objective: There is increasing evidence for the involvement of vascular disease in the pathogenesis of knee osteoarthritis. Popliteal artery wall thickness can be used as a surrogate marker of atherosclerosis. We examined the association between popliteal artery wall thickness and knee cartilage volume in individuals with symptomatic knee osteoarthritis.

Methods: This prospective cohort study analysed 176 participants from a randomised placebo-controlled trial examining the effect of atorvastatin on structural progression in knee osteoarthritis. The participants underwent magnetic resonance imaging (MRI) of the study knee at baseline and two-year follow-up. Popliteal artery wall thickness and tibial cartilage volume were measured from MRI using validated methods. The top quartile of the rate of tibial cartilage volume loss was defined as rapid progression.

Results: At baseline, every 10% increase in popliteal artery wall thickness was associated with 120.8 mm3 (95% CI 5.4-236.2, p= 0.04) lower of medial tibial cartilage volume and 151.9 mm3 (95% CI 12.1-291.7, p= 0.03) lower of lateral tibial cartilage volume. Longitudinally, for every 10% increase in popliteal artery wall thickness, the annual rate of medial tibial cartilage volume loss was increased by 1.14% (95% CI 0.09%-2.20%, p= 0.03) and there was a 2.28-fold (95% CI 1.07-4.83, p= 0.03) risk of rapid progression of medial tibial cartilage loss, adjusted for age, sex, body mass index, tibial bone area, smoking, vigorous physical activity, and intervention group allocation.

Conclusion: The findings support a role for vascular pathology in the progression of knee osteoarthritis. Targeting atherosclerosis has the potential to improve outcomes in knee osteoarthritis.
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http://dx.doi.org/10.1093/rheumatology/keac469DOI Listing
August 2022

CT Imaging Features and Clinical Characteristics of 2019 Novel Coronavirus Pneumonia (COVID-19) During Rehabilitation.

Iran J Sci Technol Trans A Sci 2022 Aug 8:1-6. Epub 2022 Aug 8.

Department of Acupuncture and Moxibustion, Wuhan First Hospital, Wuhan, 430000 Hubei People's Republic of China.

This study aims to explore the clinical characteristics of the patients with novel coronavirus pneumonia (COVID-19) during rehabilitation. One hundred and twelve confirmed patients were enrolled, while 72 were females (64.3%) and 40 were males (35.7%). The age of the patients was 51.63 ± 4.07 years old. Those patients were divided into mild group, moderate group and severe group based on lesion volume and proportion of total lesion on CT images. The age, gender, past medical history, finger pulse oxygen (SPO2), heart rate (HR) and body temperature and other clinical characteristics of patients were collected. Lesion volume was measured by CT. Compared with mild group, age, lesion volume and total lesion proportion in moderate group were significantly higher. Age, lesion volume and total lesion proportion in severe group were also higher than those in moderate group. Age and past medical history were the risk factors for the lesion volume of COVID-19. Older the patient has larger CT lesion range ( = 0.232,  = 0.045). Without past medical history or combination of post-medical history, the COVID-19 patients had smaller CT lesion ranges, and the history of previous cardiovascular disease and pulmonary disease was important risk factors for the larger CT lesion ranges. The patients who were older or combined with chronic diseases, especially cardiovascular diseases, respiratory disease and diabetes, tended to have the larger lesions. Age and past medical history of patients with COVID-19 period are significantly related to the lesion volume and total lesion proportion on CT images.
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http://dx.doi.org/10.1007/s40995-022-01338-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358081PMC
August 2022

Psychiatric risk gene transcription factor 4 preferentially regulates cortical interneuron neurogenesis during early brain development.

J Biomed Res 2022 Jul;36(4):242-254

Department of Neurobiology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Genetic variants within or near the transcription factor 4 gene ( ) are robustly implicated in psychiatric disorders including schizophrenia. However, the biological pleiotropy poses considerable obstacles to dissect the potential relationship between and those highly heterogeneous diseases. Through integrative transcriptomic analysis, we demonstrated that is preferentially expressed in cortical interneurons during early brain development. Therefore, disruptions of interneuron development might be the underlying contribution of TCF4 perturbation to a range of neurodevelopmental disorders. Here, we performed chromatin immunoprecipitation sequencing (ChIP-seq) of TCF4 on human medial ganglionic eminence-like organoids (hMGEOs) to identify genome-wide TCF4 binding sites, followed by integration of multi-omics data from human fetal brain. We observed preferential expression of the isoform TCF4-B over TCF4-A. motif analysis found that the identified 5916 TCF4 binding sites are significantly enriched for the E-box sequence. The predicted TCF4 targets in general have positively correlated expression levels with in the cortical interneurons, and are primarily involved in biological processes related to neurogenesis. Interestingly, we found that TCF4 interacts with non-bHLH proteins such as FOS/JUN, which may underlie the functional specificity of TCF4 in hMGEOs. This study highlights the regulatory role of TCF4 in interneuron development and provides compelling evidence to support the biological rationale linking to the developing cortical interneuron and psychiatric disorders.
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http://dx.doi.org/10.7555/JBR.36.20220074DOI Listing
July 2022

Development and preliminary validation of a public health emergency competency model for medical staffs of national health emergency teams in China.

BMC Health Serv Res 2022 Aug 13;22(1):1033. Epub 2022 Aug 13.

Department of Psychology, Weifang Medical University, Fuyanshan Campus, No. 7166, West Baotong Street, Weicheng District, Weifang, 261053, Shandong, China.

Background: In the present study, we attempted to develop and validate a participatory competency model for medical workers and then evaluate the current status of competency characteristics of Chinese medical workers.

Methods: The competency model was constructed in a multistage process, including literature review, expert consultation, critical incident and focus group interview. A pilot study was conducted to refine the initial model among 90 participators and the viability and reliability were evaluated by a questionnaire survey among 121 medical workers. Then, the current status of competency characteristics was measured based on the final version of competency model.

Results: In the pilot study, ten questionnaires were dropped for the poor quality and thus the eligible rate was 92% (138/150). KMO value was 0.785 and Bartlett test showed that the χ = 6464.546 (df = 903) and p value < 0.001. Then, 10 items with double loading and factor loading < 0.4 were deleted. Finally, 33 items were retained with the lowest factor loading value of 0.465. The validity and reliability of competency model were determined with Cronbach's α coefficient of 0.975 and ICC value of 0.933. Finally, a revised competency model with 5 dimensions and 31 items was obtained. The overall competencies of current medical workers were in a high level, except for emergency knowledge related competencies. Age was an independent factor affecting the competencies.

Conclusions: Our competency model was a reliable and validated tool for assessing the competences of medical staffs against public health emergencies, and the overall competencies of current medical workers in China were in a high level, except for emergency knowledge related competencies.
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http://dx.doi.org/10.1186/s12913-022-08361-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374485PMC
August 2022

Neutrophils activated by membrane attack complexes increase the permeability of melanoma blood vessels.

Proc Natl Acad Sci U S A 2022 Aug 12;119(33):e2122716119. Epub 2022 Aug 12.

Department of Dermatology and Venereology, University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany.

The microenvironment of malignant melanomas defines the properties of tumor blood vessels and regulates infiltration and vascular dissemination of immune and cancer cells, respectively. Previous research in other cancer entities suggested the complement system as an essential part of the tumor microenvironment. Here, we confirm activation of the complement system in samples of melanoma patients and murine melanomas. We identified the tumor endothelium as the starting point of the complement cascade. Generation of complement-derived C5a promoted the recruitment of neutrophils. Upon contact with the vascular endothelium, neutrophils were further activated by complement membrane attack complexes (MACs). MAC-activated neutrophils release neutrophil extracellular traps (NETs). Close to the blood vessel wall, NETs opened the endothelial barrier as indicated by an enhanced vascular leakage. This facilitated the entrance of melanoma cells into the circulation and their systemic spread. Depletion of neutrophils or lack of MAC formation in complement component 6 (C6)-deficient animals protected the vascular endothelium and prevented vascular intravasation of melanoma cells. Our data suggest that inhibition of MAC-mediated neutrophil activation is a potent strategy to abolish hematogenous dissemination in melanoma.
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http://dx.doi.org/10.1073/pnas.2122716119DOI Listing
August 2022

Case report: Two novel fusions in non-small-cell lung cancer resistant to alectinib: A report of two cases.

Front Oncol 2022 22;12:916315. Epub 2022 Jul 22.

Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: The anaplastic lymphoma kinase () mutation, also known as the diamond mutation in non-small-cell lung cancer (NSCLC), has been treated with tremendous success since it was first reported in 2007. Alectinib, a second generation ALK-Tyrosine kinase inhibitor (TKI), has been reported to have significantly longer progression- free survival (PFS) than first generation ALK inhibitors in untreated ALK positive NSCLC. However, the clinical efficacy of ALK-TKIs on rare ALK fusions remains unclear. In recent years, with the popularity of next-generation sequencing (NGS) technology, an increasing number of novel ALK fusion partners have been reported, but the responses are heterogeneous among different ALK fusions. Considering the inconsistent reactions, the clinical efficacy of ALK-TKIs in rare ALK gene fusions remains to be evaluated in more cases.

Methods: To seek for individualized therapy, the tumor tissues acquired during biopsy were sent for genomic testing by NGS based on a 139-gene panel and a 425-gene panel in a centralized clinical testing center (GENESEEQ Technology Inc, Nanjing, China). See for more details about the methods for DNA-based NGS, RNA-based NGS.

Results: We present two cases of patients with lung adenocarcinoma harboring two novel () rearrangements detected by DNA sequencing, which had limited clinical response to ALK-TKIs but showed sensitivity to chemotherapy combined with bevacizumab therapy in patient 2, with a PFS of over 1 year up till the last follow-up assessment.

Conclusions: In summary, our cases emphasize the need for comprehensive molecular analysis of different ALK fusion partners at the DNA level to formulate accurate treatment strategies and provide a certain therapeutic reference for these two types of novel fusions.
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http://dx.doi.org/10.3389/fonc.2022.916315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356229PMC
July 2022

Effects of ultrasound pretreatment at different powers on flavor characteristics of enzymatic hydrolysates of cod (Gadus macrocephalus) head.

Food Res Int 2022 Sep 3;159:111612. Epub 2022 Jul 3.

College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, Liaoning 116034, China. Electronic address:

To make better use of cod head (Gadus macrocephalus), a by-product of fish processing, the effects of ultrasound pretreatment on the enzymatic properties and volatile compounds of cod head enzymatic hydrolysates were investigated. The results demonstrated that ultrasound pretreatment at 150-600 W had a positive effect cod head enzymatic hydrolysates. The soluble peptides content of the enzymatic hydrolysates reached the highest value of 5.31 ± 0.16 mg/mL at the ultrasound power level of 450 W, and the content of peptide molecules < 3-kDa was up to 93.96%. The type and relative content of volatile compounds, especially aldehydes, alcohols, and ketones, also increased with the increase the ultrasound power. The electronic tongue results indicated that ultrasound pretreatment reduced bitterness and astringency. The electronic nose results indicated that the hydrogen- and alkane-containing odor components in the hydrolyzed liquid after ultrasound pretreatment differed significantly from conventional enzymatic hydrolysates. In conclusion, ultrasound pretreatment may be applicable as a suitable technology to assist enzymatic hydrolysis of the cod head, and as such, promote the utilization of fish by-products.
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http://dx.doi.org/10.1016/j.foodres.2022.111612DOI Listing
September 2022

Ecological risk assessment and identification of the distinct microbial groups in heavy metal-polluted river sediments.

Environ Geochem Health 2022 Aug 8. Epub 2022 Aug 8.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 610031, China.

To assess the health of river ecosystems, it is essential to quantify the ecological risk of heavy metals in river sediments and the structure of microbial communities. As important tributaries of the Tuo River in the upper reaches of the Yangtze River, the Mianyuan River and the Shiting River, are closely related to the economic development and human daily life in the region. This study assessed the ecological risks of heavy-metal-polluted river sediments, the heavy-metal-driven bacterial communities were revealed, and the relationships between the ecological risks and the identical bacterial communities were discussed. The Cd content was significantly greater than the environmental background value, leading to a serious pollution and very high ecological risk at the confluence of the two rivers and the upper reaches of the Mianyuan River. Microbial community analysis showed that Rhodobacter, Nocardioides, Sphingomonas, and Pseudarthrobacter were the dominant bacterial genera in the sediments of the Shiting River. However, the dominant bacterial genera in the Mianyuan River were Kouleothrix, Dechloromonas, Gaiella, Pedomicrobium, and Hyphomicrobium. Mantel test results showed (r = 0.5977, P = 0.005) that the Cd, As, Zn, Pb, Cr, and Cu were important factors that influenced differences in the distribution of sediment bacterial communities Mianyuan and Shiting rivers. A correlation heatmap showed that heavy metals were negatively correlated for most bacterial communities, but some bacterial communities were tolerant and showed a positive correlation. Overall, the microbial structure of the river sediments showed a diverse spatial distribution due to the influence of heavy metals. The results will improve the understanding of rivers contaminated by heavy metals and provide theoretical support for conservation and in situ ecological restoration of river ecosystems.
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http://dx.doi.org/10.1007/s10653-022-01343-4DOI Listing
August 2022

Initial Experience With Transoral Endoscopic Thyroidectomy via the Submental and Vestibular Approach for the Treatment of Thyroid Cancer: A Retrospective Cohort Study.

Front Surg 2022 20;9:882150. Epub 2022 Jul 20.

Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, China.

Background: Transoral endoscopic thyroidectomy vestibular approach is feasible and safe but has some unavoidable limitations, such as sensory changes in the center of the chin region. We aim to report our initial experience in performing transoral endoscopic thyroidectomy via the submental and vestibular approach for the treatment of thyroid cancer.

Patients And Methods: This retrospective cohort study included patients with thyroid cancer confirmed by fine-needle aspiration who underwent endoscopic thyroidectomy and central lymph node dissection via the submental and vestibular approaches between November 2019 and January 2020. Patients' clinicopathological characteristics, operation details, and postoperative complications were analyzed.

Results: Fifteen surgeries were performed successfully. The mean ± standard deviation age of the patients was 37 ± 10.8 years, the average duration of surgery was 146.5 ± 34.6 min, and the median intraoperative blood loss was 11.1 ± 6.3 mL. None of the surgeries were converted to open thyroidectomy. According to postoperative pathology, all cases involved papillary thyroid carcinoma or papillary thyroid microcarcinoma. One patient developed transient recurrent laryngeal nerve paralysis. No patient developed skin numbness at the center of the chin region.

Conclusions: Transoral endoscopic thyroidectomy via the submental and vestibular approach is effective and safe in patients with thyroid cancer and does not lead to skin numbness at the center of the chin region. This technique is beneficial for surgeons less experienced in performing transoral thyroid surgery as it involves using a short and direct route to the thyroid gland, which can reduce the difficulty in establishing the first operative space to some extent.
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http://dx.doi.org/10.3389/fsurg.2022.882150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346076PMC
July 2022

Age-specific random day serum anti-Mullerian hormone reference values for women of reproductive age in the general population: a large Chinese nationwide population-based survey.

Am J Obstet Gynecol 2022 Aug 4. Epub 2022 Aug 4.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China. Electronic address:

Background: Anti-Mullerian hormone, the most reliable biomarker of ovarian reserve, is widely used in various clinical situations. Anti-Mullerian hormone levels consistently decrease with age. However, there are no standard, age-specific reference values for anti-Mullerian hormone in women of reproductive age, which limits its application.

Objective: To establish age-specific anti-Mullerian hormone centile reference values for women of reproductive age.

Study Design: A nationwide, population-based cross-sectional survey was conducted between May 2019 and April 2021 in 15 provinces/municipalities in mainland China. A total of 10,053 eligible women aged 20-49 years were selected using a multistage stratified sampling procedure. Women who were pregnant, had undergone ovarian surgery, took hormone drugs in the past 3 months, or had an anti-Mullerian hormone outlier value were excluded in establishing anti-Mullerian hormone centile reference values. Serum anti-Mullerian hormone concentrations were measured using ultra-sensitive, two-site enzyme-linked immunosorbent assays (Ansh Lab) in the Reproductive Endocrinology Laboratory of Peking University Third Hospital. Generalized additive models for location scale and shape with the Box-Cox t original distribution were used to estimate the fitted anti-Mullerian hormone centile reference values.

Results: A total of 9,112 eligible women aged 21-49 years were included in the fitting model. The fitted 50th (2.5th-97.5th) centile of anti-Mullerian hormone values for women aged 21, 25, 30, 35, 40, 45, and 49 years were 4.83 (0.79-18.41), 4.47 (0.72-16.58), 3.67 (0.50-13.82), 2.59 (0.24-10.35), 1.35 (0.05-6.68), 0.33 (<0.01-3.40), and 0.04 (<0.01-1.77) ng/ml, respectively. The population-based decline rate in anti-Mullerian hormone accelerated with increasing age, especially age over 35 years. The magnitude of decline of the 25th anti-Mullerian hormone centile curve was greater than that of the 75th centile curve.

Conclusions: We established age-specific anti-Mullerian hormone centile reference values for women of reproductive age based on a large representative sample of the general population and described anti-Mullerian hormone changes. Our findings may facilitate anti-Mullerian hormone application in clinical practices.
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http://dx.doi.org/10.1016/j.ajog.2022.07.029DOI Listing
August 2022

miRNA-338-3p inhibits the migration, invasion and proliferation of human lung adenocarcinoma cells by targeting MAP3K2.

Aging (Albany NY) 2022 Aug 3;14(undefined). Epub 2022 Aug 3.

Department of Respiratory Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: This study aimed to investigate the effects of micro ribonucleic acid (miR)-338-3p on the migration, invasion and proliferation of lung adenocarcinoma (LUAD) cells.

Methods: Bioinformatics analysis was employed to evaluate the function and expression of related genes in lung cancer. Human A549 and NCI-H1299 cells cultured to logarithmic growth stage were assigned to negative control (NC) mimic group, miR-338-3p mimic group (miR-mimic group), NC inhibitor group and miR-338-3p inhibitor group (miR-inhibitor group) treated with or without MAP3K2 overexpression (OE)-lentivirus, or TBHQ or FR180204. Transwell assay, cell colony formation assay, Western blotting and cell-cycle analysis were carried out.

Results: Bioinformatics results manifested that miR-338 and MAP3K2 were involved in LUAD. The expression levels of MAP3K2, p-ERK1/2, MMP-2, MMP-3, MMP-9, cyclin A2 and cyclin D1 were increased after addition of miR-338-3p inhibitor, consistent with the raised amount of LUAD cells in migration and invasion experiments and number of colonies formed, as well as the cell cycle, but miR-338-3p mimic reversed these results. Moreover, MAP3K2 overexpression elevated the level of p-ERK1/2. Meanwhile, after treatment with TBHQ or FR180204, the influence of miR-338-3p inhibitor or mimic was also verified.

Conclusions: MiR-338-3p overexpression can modulate the ERK1/2 signaling pathway by targeting MAP3K2, thus inhibiting the migration, invasion and proliferation of human LUAD cells.
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http://dx.doi.org/10.18632/aging.204198DOI Listing
August 2022

Prognosis of Congenital Anomalies in Conceptions Following Fertilization: A Multicenter Retrospective Cohort Study in China.

Front Endocrinol (Lausanne) 2022 14;13:900499. Epub 2022 Jul 14.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Background: Conceptions following fertilization (IVF) or intracytoplasmic sperm injection (ICSI) have an increased risk of congenital anomalies. Few studies have explored the prognosis of fetuses with congenital anomalies. This study aimed to investigate the prevalence and prognosis of congenital anomalies in IVF/ICSI pregnancies, and to analyze the influencing factors contributing to poor prognosis.

Methods: In this multicenter retrospective cohort study, we followed 405,473 embryo transfer cycles at 15 reproductive centers between January 2010 and December 2019 and enrolled 2,006 intrauterine pregnancies with congenital anomalies. The relatively positive prognosis group with one or more live births and neonatal survival for more than 7 days was compared with the poor prognosis group with poorer outcomes.

Results: Among the 168,270 ongoing intrauterine pregnancy cycles, the prevalence of congenital anomalies was 1.19%, wherein the malformation rates of cycles with late abortion and delivery were 2.37% (716/30,202) and 0.93% (1,290/138,068), respectively. Among all IVF/ICSI cycles with congenital anomalies, the relatively positive prognosis rate was 61.39%. Moreover, the fertilization failure rate (2 pro-nuclei rate < 25%) in the poor prognosis group was significantly higher than that in the relatively positive prognosis group (10.89% vs. 5.09%, < 0.001). Multivariate logistic regression analysis revealed no significant differences in the relatively positive prognosis rate among the various IVF/ICSI protocols. The relatively positive prognosis rate of fertilization failure cycles was 0.180 times that of normal fertilization cycles.

Conclusion: Poor fertilization rates during IVF/ICSI treatments are more likely to have poor prognosis in fetuses or neonates with congenital anomalies, and obstetric management should be strengthened in pregnant women, with which pregnant women should be recommended to strengthen obstetric management.
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http://dx.doi.org/10.3389/fendo.2022.900499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331169PMC
August 2022

The role of selenoprotein P in the determining the sensitivity of cervical cancer patients to concurrent chemoradiotherapy: A metabonomics-based analysis.

J Trace Elem Med Biol 2022 Sep 18;73:127041. Epub 2022 Jul 18.

Institute of Keshan Disease, Chinese Center for Endemic Disease Control, Harbin Medical University, Harbin 150081, China. Electronic address:

Background: The effect of selenoprotein P (SELENOP) levels on the sensitivity of cervical cancer patients to concurrent chemoradiotherapy (CCRT) has not been reported. In this study, the effects of the variations in plasma SELENOP levels on the sensitivity of cervical cancer patients to CCRT were investigated using metabonomics.

Methods: Two patient groups were evaluated, i.e., the case group: 11 patients with intermediate to advanced primary squamous cervical cancer, who developed resistance against CCRT, and the sensitivity group: 11 patients who did not develop resistance were matched in a 1:1 ratio (controls). Blood samples were collected before and after CCRT, and the plasma SELENOP levels were measured by ELISA. The different metabolites present in the plasma were analyzed by UPLC-MS-MS.

Results: SELENOP levels exhibited a significant reduction in both the resistant and sensitive groups after CCRT (F = 50.705, P < 0.001), and interaction effects between sensitivity and pre-and post-treatment on SELENOP levels were observed (F = 7.414, P = 0.013). Further, a more significant reduction in the SELENOP levels was observed in the CCRT-resistant group (mean reduction, 0.028 µg/mL; P < 0.001) than in the sensitive group (mean reduction, 0.013 µg/mL; P = 0.006). Four metabolic biomarkers, i.e., C18, C19, C20 sphingomyelin, and phosphatidylcholine 20:2/22:6, were shown to be differentially expressed between the resistant and sensitive groups pre-and post-treatment. C20 sphingomyelin levels exhibited a significant correlation with SELENOP levels (r = -0.326, P = 0.031).

Conclusion: The levels of plasma SELENOP in the CCRT-resistant group decreased significantly, suggesting that SELENOP might affect the sensitivity by modulating lipid synthesis and metabolism.
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http://dx.doi.org/10.1016/j.jtemb.2022.127041DOI Listing
September 2022

Comparative global B cell receptor repertoire difference induced by SARS-CoV-2 infection or vaccination via single-cell V(D)J sequencing.

Emerg Microbes Infect 2022 Dec;11(1):2007-2020

School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, People's Republic of China.

Dynamic changes of the paired heavy and light chain B cell receptor (BCR) repertoire provide an essential insight into understanding the humoral immune response post-SARS-CoV-2 infection and vaccination. However, differences between the endogenous paired BCR repertoire kinetics in SARS-CoV-2 infection and previously recovered/naïve subjects treated with the inactivated vaccine remain largely unknown. We performed single-cell V(D)J sequencing of B cells from six healthy donors with three shots of inactivated SARS-CoV-2 vaccine (BBIBP-CorV), five people who received the BBIBP-CorV vaccine after having recovered from COVID-19, five unvaccinated COVID-19 recovered patients and then integrated with public data of B cells from four SARS-CoV-2-infected subjects. We discovered that BCR variable (V) genes were more prominently used in the SARS-CoV-2 exposed groups (both in the group with active infection and in the group that had recovered) than in the vaccinated groups. The VH gene that expanded the most after SARS-CoV-2 infection was IGHV3-33, while IGHV3-23 in the vaccinated groups. SARS-CoV-2-infected group enhanced more BCR clonal expansion and somatic hypermutation than the vaccinated healthy group. A small proportion of public clonotypes were shared between the SARS-CoV-2 infected, vaccinated healthy, and recovered groups. Moreover, several public antibodies had been identified against SARS-CoV-2 spike protein. We comprehensively characterize the paired heavy and light chain BCR repertoire from SARS-CoV-2 infection to vaccination, providing further guidance for the development of the next-generation precision vaccine.
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http://dx.doi.org/10.1080/22221751.2022.2105261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377262PMC
December 2022

Ultrasound-based radiomics technology in fetal lung texture analysis prediction of neonatal respiratory morbidity.

Sci Rep 2022 Jul 26;12(1):12747. Epub 2022 Jul 26.

Obstetrics and Gynecology Hospital of Fudan University, No.128, Shenyang Road, Shanghai, 200090, China.

To develop a novel method for predicting neonatal respiratory morbidity (NRM) by ultrasound-based radiomics technology. In this retrospective study, 430 high-throughput features per fetal-lung image were extracted from 295 fetal lung ultrasound images (four-chamber view) in 295 single pregnancies. Images had been obtained between 28 and 37 weeks of gestation within 72 h before delivery. A machine-learning model built by RUSBoost (Random under-sampling with AdaBoost) architecture was created using 20 radiomics features extracted from the images and 2 clinical features (gestational age and pregnancy complications) to predict the possibility of NRM. Of the 295 standard fetal lung ultrasound images included, 210 in the training set and 85 in the testing set. The overall performance of the neonatal respiratory morbidity prediction model achieved AUC of 0.88 (95% CI 0.83-0.92) in the training set and 0.83 (95% CI 0.79-0.97) in the testing set, sensitivity of 84.31% (95% CI 79.06-89.44%) in the training set and 77.78% (95% CI 68.30-87.43%) in the testing set, specificity of 81.13% (95% CI 78.16-84.07%) in the training set and 82.09% (95% CI 77.65-86.62%) in the testing set, and accuracy of 81.90% (95% CI 79.34-84.41%) in the training set and 81.18% (95% CI 77.33-85.12%) in the testing set. Ultrasound-based radiomics technology can be used to predict NRM. The results of this study may provide a novel method for non-invasive approaches for the prenatal prediction of NRM.
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http://dx.doi.org/10.1038/s41598-022-17129-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325724PMC
July 2022

The clinical significance of BRAFV600E mutations in pediatric papillary thyroid carcinomas.

Sci Rep 2022 Jul 25;12(1):12674. Epub 2022 Jul 25.

Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

This study aimed to review the clinical significance of BRAFV600E mutations in pediatric papillary thyroid carcinoma (PTC). From 2018 to 2021, 392 pediatric thyroid operations were performed in the first affiliated Hospital of Zhengzhou University. Of these, 169 patients underwent their first operation in our hospital and were histopathologically diagnosed as papillary thyroid carcinoma. BRAFV600E gene mutation detection was performed in these 169 pediatric patients to investigate the correlation between BRAF gene mutations and clinicopathological features. Ninety-seven of our 169 patients had a BRAFV600E mutation, with a mutation rate of 57.4%. The incidence of BRAFV600E was higher in boys than in girls, and in the 13-18-year age group as compared with the 6-12-year age group (P < 0.05). The positivity rate of BRAFV600E in unilateral PTC (67.7%) was significantly higher than the ones in bilateral PTC (28.9%). The occurrence of diffuse microcalcification of the thyroid negatively correlated with the presence of BRAFV600E mutations. BRAFV600E mutations were found more frequently in patients with smaller tumor size, a lack of multifocality, lower TSH levels and central lymph node metastasis. During the follow-up time, 70 patients were treated with iodine-131. Eight patients required a second surgery (All had cervical lymph node recurrence). BRAFV600E mutations do not suggest a more aggressive course in papillary thyroid carcinoma in pediatric patients in the short term.
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http://dx.doi.org/10.1038/s41598-022-16207-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314322PMC
July 2022

Effects and Prognostic Values of Circadian Genes CSNK1E/GNA11/KLF9/THRAP3 in Kidney Renal Clear Cell Carcinoma via a Comprehensive Analysis.

Bioengineering (Basel) 2022 Jul 11;9(7). Epub 2022 Jul 11.

School of Life Science, Bengbu Medical College, Bengbu 233030, China.

Kidney renal clear cell carcinoma (KIRC) is one of the most prevalent and deadly types of renal cancer in adults. Recent research has identified circadian genes as being involved in the development and progression of KIRC by altering their expression. This study aimed to identify circadian genes that are differentially expressed in KIRC and assess their role in KIRC progression. In KIRC, there were 553 differentially expressed rhythm genes (DERGs), with 300 up-regulated and 253 down-regulated DERGs. Functional enrichment analyses showed that DERGs were greatly enriched in the circadian rhythm and immune response pathways. Survival analyses indicated that higher expression levels of CSNK1E were related to shorter overall survival of KIRC patients, whereas lower expression levels of GNA11, KLF9, and THRAP3 were associated with shorter overall survival of KIRC patients. Through cell assay verification, the mRNA level of CSNK1E was significantly up-regulated, whereas the mRNA levels of GNA11, KLF9, and THRAP3 were dramatically down-regulated in KIRC cells, which were consistent with the bioinformatics analysis of KIRC patient samples. Age, grade, stage, TM classification, and CSNK1E expression were all shown to be high-risk variables, whereas GNA11, KLF9, and THRAP3 expression were found to be low-risk factors in univariate Cox analyses. Multivariate Cox analyses showed that CSNK1E and KLF9 were also independently related to overall survival. Immune infiltration analysis indicated that the proportion of immune cells varied greatly between KIRC tissues and normal tissue, whereas CSNK1E, GNA11, KLF9, and THRAP3 expression levels were substantially linked with the infiltration abundance of immune cells and immunological biomarkers. Moreover, interaction networks between CSNK1E/GNA11/KLF9/THRAP3 and immune genes were constructed to explore the stream connections. The findings could help us better understand the molecular mechanisms of KIRC progression, and CSNK1E/GNA11/KLF9/THRAP3 might be used as molecular targets for chronotherapy in KIRC patients in the near future.
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http://dx.doi.org/10.3390/bioengineering9070306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311602PMC
July 2022

Effects and Prognostic Values of Circadian Genes CSNK1E/GNA11/KLF9/THRAP3 in Kidney Renal Clear Cell Carcinoma via a Comprehensive Analysis.

Bioengineering (Basel) 2022 Jul 11;9(7). Epub 2022 Jul 11.

School of Life Science, Bengbu Medical College, Bengbu 233030, China.

Kidney renal clear cell carcinoma (KIRC) is one of the most prevalent and deadly types of renal cancer in adults. Recent research has identified circadian genes as being involved in the development and progression of KIRC by altering their expression. This study aimed to identify circadian genes that are differentially expressed in KIRC and assess their role in KIRC progression. In KIRC, there were 553 differentially expressed rhythm genes (DERGs), with 300 up-regulated and 253 down-regulated DERGs. Functional enrichment analyses showed that DERGs were greatly enriched in the circadian rhythm and immune response pathways. Survival analyses indicated that higher expression levels of CSNK1E were related to shorter overall survival of KIRC patients, whereas lower expression levels of GNA11, KLF9, and THRAP3 were associated with shorter overall survival of KIRC patients. Through cell assay verification, the mRNA level of CSNK1E was significantly up-regulated, whereas the mRNA levels of GNA11, KLF9, and THRAP3 were dramatically down-regulated in KIRC cells, which were consistent with the bioinformatics analysis of KIRC patient samples. Age, grade, stage, TM classification, and CSNK1E expression were all shown to be high-risk variables, whereas GNA11, KLF9, and THRAP3 expression were found to be low-risk factors in univariate Cox analyses. Multivariate Cox analyses showed that CSNK1E and KLF9 were also independently related to overall survival. Immune infiltration analysis indicated that the proportion of immune cells varied greatly between KIRC tissues and normal tissue, whereas CSNK1E, GNA11, KLF9, and THRAP3 expression levels were substantially linked with the infiltration abundance of immune cells and immunological biomarkers. Moreover, interaction networks between CSNK1E/GNA11/KLF9/THRAP3 and immune genes were constructed to explore the stream connections. The findings could help us better understand the molecular mechanisms of KIRC progression, and CSNK1E/GNA11/KLF9/THRAP3 might be used as molecular targets for chronotherapy in KIRC patients in the near future.
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http://dx.doi.org/10.3390/bioengineering9070306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311602PMC
July 2022

Deep Learning of Liver Contrast-Enhanced Ultrasound to Predict Microvascular Invasion and Prognosis in Hepatocellular Carcinoma.

Front Oncol 2022 7;12:878061. Epub 2022 Jul 7.

Department of Ultrasound, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background And Aims: Microvascular invasion (MVI) is a well-known risk factor for poor prognosis in hepatocellular carcinoma (HCC). This study aimed to develop a deep convolutional neural network (DCNN) model based on contrast-enhanced ultrasound (CEUS) to predict MVI, and thus to predict prognosis in patients with HCC.

Methods: A total of 436 patients with surgically resected HCC who underwent preoperative CEUS were retrospectively enrolled. Patients were divided into training ( = 301), validation ( = 102), and test ( = 33) sets. A clinical model (Clinical model), a CEUS video-based DCNN model (CEUS-DCNN model), and a fusion model based on CEUS video and clinical variables (CECL-DCNN model) were built to predict MVI. Survival analysis was used to evaluate the clinical performance of the predicted MVI.

Results: Compared with the Clinical model, the CEUS-DCNN model exhibited similar sensitivity, but higher specificity (71.4% vs. 38.1%, = 0.03) in the test group. The CECL-DCNN model showed significantly higher specificity (81.0% vs. 38.1%, = 0.005) and accuracy (78.8% vs. 51.5%, = 0.009) than the Clinical model, with an AUC of 0.865. The Clinical predicted MVI could not significantly distinguish OS or RFS (both > 0.05), while the CEUS-DCNN predicted MVI could only predict the earlier recurrence (hazard ratio [HR] with 95% confidence interval [CI 2.92 [1.1-7.75], = 0.024). However, the CECL-DCNN predicted MVI was a significant prognostic factor for both OS (HR with 95% CI: 6.03 [1.7-21.39], = 0.009) and RFS (HR with 95% CI: 3.3 [1.23-8.91], = 0.011) in the test group.

Conclusions: The proposed CECL-DCNN model based on preoperative CEUS video can serve as a noninvasive tool to predict MVI status in HCC, thereby predicting poor prognosis.
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http://dx.doi.org/10.3389/fonc.2022.878061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300962PMC
July 2022

Direct Patterning of Colloidal Nanocrystals Thermally Activated Ligand Chemistry.

ACS Nano 2022 Jul 22. Epub 2022 Jul 22.

Department of Chemistry, Center for BioAnalytical Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China.

Precise patterning with microscale lateral resolution and widely tunable heights is critical for integrating colloidal nanocrystals into advanced optoelectronic and photonic platforms. However, patterning nanocrystal layers with thickness above 100 nm remains challenging for both conventional and emerging direct photopatterning methods, due to limited light penetration depths, complex mechanical and chemical incompatibilities, and others. Here, we introduce a direct patterning method based on a thermal mechanism, namely, the thermally activated ligand chemistry (or TALC) of nanocrystals. The ligand cross-linking or decomposition reactions readily occur under local thermal stimuli triggered by near-infrared lasers, affording high-resolution and nondestructive patterning of various nanocrystals under mild conditions. Patterned quantum dots fully preserve their structural and photoluminescent quantum yields. The thermal nature allows for TALC to pattern over 10 μm thick nanocrystal layers in a single step, far beyond those achievable in other direct patterning techniques, and also supports the concept of 2.5D patterning. The thermal chemistry-mediated TALC creates more possibilities in integrating nanocrystal layers in uniform arrays or complex hierarchical formats for advanced capabilities in light emission, conversion, and modulation.
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http://dx.doi.org/10.1021/acsnano.2c04033DOI Listing
July 2022

Microglia homeostasis mediated by epigenetic ARID1A regulates neural progenitor cells response and leads to autism-like behaviors.

Mol Psychiatry 2022 Jul 20. Epub 2022 Jul 20.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China.

Microglia are resident macrophages of the central nervous system that selectively emerge in embryonic cortical proliferative zones and regulate neurogenesis by altering molecular and phenotypic states. Despite their important roles in inflammatory phagocytosis and neurodegenerative diseases, microglial homeostasis during early brain development has not been fully elucidated. Here, we demonstrate a notable interplay between microglial homeostasis and neural progenitor cell signal transduction during embryonic neurogenesis. ARID1A, an epigenetic subunit of the SWI/SNF chromatin-remodeling complex, disrupts genome-wide H3K9me3 occupancy in microglia and changes the epigenetic chromatin landscape of regulatory elements that influence the switching of microglial states. Perturbation of microglial homeostasis impairs the release of PRG3, which regulates neural progenitor cell self-renewal and differentiation during embryonic development. Furthermore, the loss of microglia-driven PRG3 alters the downstream cascade of the Wnt/β-catenin signaling pathway through its interaction with the neural progenitor receptor LRP6, which leads to misplaced regulation in neuronal development and causes autism-like behaviors at later stages. Thus, during early fetal brain development, microglia progress toward a more homeostatic competent phenotype, which might render neural progenitor cells respond to environmental cross-talk perturbations.
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http://dx.doi.org/10.1038/s41380-022-01703-7DOI Listing
July 2022

Effects of Ketogenic Diet on Neuroinflammation in Neurodegenerative Diseases.

Aging Dis 2022 Jul 11;13(4):1146-1165. Epub 2022 Jul 11.

Department of Neurology, The Second Medical Center and National Clinical Research Center for Geriatric Disease, Chinese PLA General Hospital, Beijing 100853, China.

The ketogenic diet (KD) is a low-carbohydrate, high-fat and adequate-protein diet. As a diet mimicking fasting, it triggers the production of ketone bodies (KBs) and brings the body into a state of ketosis. Recent and accumulating studies on humans and animal models have shown that KD is beneficial to neurodegenerative diseases through modulating central and peripheral metabolism, mitochondrial function, inflammation, oxidative stress, autophagy, and the gut microbiome. Complicated interplay of metabolism, gut microbiome, and other mechanisms can regulate neuroinflammation in neurodegenerative diseases by activating multiple molecular and cellular pathways. In this review, we detail the physiological basis of the KD, its functions in regulating neuroinflammation, and its protective role in normal brain aging and neurodegenerative diseases, such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington's disease (HD). We aimed to elucidate the underlying neuroinflammatory mechanisms of KD therapies in neurodegenerative diseases and provide novel insights into their application for neurodegenerative disease prevention and treatment.
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http://dx.doi.org/10.14336/AD.2021.1217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286903PMC
July 2022

Structural engineering brings new electronic properties to Janus ZrSSe and HfSSe monolayers.

Phys Chem Chem Phys 2022 Jul 27;24(29):17824-17831. Epub 2022 Jul 27.

School of Physics, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.

Interfacing effects within emergent two-dimensional (2D) materials are of fundamental interest and are at the center of applications in nanoelectronics. Thus, out-of-plane and in-plane heterostructures as well as electronic heterostructures with phase boundaries and large-angle (60°) grain boundaries (GBs) of Janus ZrSSe and HfSSe are studied in this work using first-principles calculations. The out-of-plane heterostructures of T-ZrSSe and T-HfSSe illustrate quite weak interfacing interactions, thus the electronic properties are, unusually, more like the superposition of individual monolayers. The in-plane heterostructures of T-ZrSSe and T-HfSSe, interestingly, exhibit an indirect-direct band gap transition and type-II band alignment, which correspond to boosted optical properties and spatially separated excitons. For the in-plane electronic heterostructures that are constituted by T-ZrSSe and H-ZrSSe, semiconductor-metal crossover occurs due to the polar discontinuity across the T-H phase boundary, and they behave as one-dimensional metallic wires embedded in otherwise semiconducting Janus ZrSSe, creating a one-dimensional electron/hole gas. This also indicates a strategy for stabilizing the unstable and/or metastable monolayer the phase boundary. As a result of the zero formal bulk polarization of the T-phase ZrSSe, the metallicity of 60° GBs originates mainly from the edge atoms rather than from the polar discontinuity.
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http://dx.doi.org/10.1039/d2cp01928kDOI Listing
July 2022

Structural network alterations induced by ART-naive and ART-treated subjects infected with HIV.

Biochem Biophys Res Commun 2022 09 30;622:115-121. Epub 2022 Jun 30.

Beijign Youan Hospital, Capital Medical University, Beijing, 100069, China. Electronic address:

Objective: To investigate how the structural connectivity altered in combined antiretroviral therapy-treated (cART+) HIV patients and cART-naive (cART-) HIV patients by conducting Network analysis of Diffusion Tensor Imaging (DTI) data.

Methods: We enrolled 22 cART-, 23 cART+ and 28 normal controls (NC) in our current study. Firstly, the DTI imaging data pre-processing was conducted and the asymmetric 90 × 90 matrix for each participant from their DTI data was obtained with the use of PANDA. Then, we applied a graph-theoretical network analysis toolkit, GRETNA v2.0, to calculate metrics such as small-"worldness," characteristic path length, clustering coefficient, global efficiency, local efficiency, and nodal "betweenness". Finally, we took comparisons among the three groups to investigate topological alterations.

Results: Results (1) the regional characteristics (nodal efficiency) were altered in cART- and cART+ patients predominantly in the frontal cortical regions; (2) changes in various network properties in cART+treat and cART-patients were associated with the performance of behavior functions; (3) Hubs redistributed in HIV subjects especially in cART+ patients.

Conclusion: The regional characteristics (nodal efficiency) were altered in cART- and cART+ patients predominantly in the frontal cortical region, and changes in various network properties in cART- and cART+ patients were associated with the performance of behavior functions. In addition, Hubs redistributed in HIV subjects especially in cART+ patients.
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http://dx.doi.org/10.1016/j.bbrc.2022.06.078DOI Listing
September 2022

Shenqihuatan formula reduces inflammation by inhibiting transforming growth factor-beta-stimulated signaling pathway in airway smooth muscle cells.

J Tradit Chin Med 2022 Aug;42(4):520-529

Shanxi Provincial Traditional Chinese Medicine Hospital, Taiyuan 030012, China.

Objective: To study the effects and mechanism of Shenqihuatan formula (, SQHT) of the transforming growth factor-beta (TGF-β)-stimulated cell processes in airway remodeling.

Methods: The current study examined cell viability using a Cell Counting Kit-8 assay. Furthermore, a Transwell assay was conducted to detect the ability of cell migration, and apoptosis was detected via flowcytometry. Western Blot and quantitative real-time polymerase chain reaction (qRT-PCR) were used to determine the expression levels of apoptosis or inflammation-related factors, such as TGF-β, Interleukin-1β (IL-1β), B cell lymphoma 2 (Bcl-2), Bcl-2-Associated X (Bax), Ras homolog gene family, member A (RhoA), recombinant rho associated coiled coil containing protein kinase 1/2 (ROCK1/2), extracellular regulated protein kinases 1/2 (ERK1/2), Snail, and Slug. Finally, the expression levels of matrix metalloproteinase-9 (MMP-9) and Tissue inhibitor of metalloproteinase (TIMP-1) were admeasured by enzyme-linked immuno sorbent assay.

Results: The results demonstrated that SQHT inhibited the viability and migration, as well as the the F-actin formation and cytoskeletal reorganization of airway smooth muscle cells (ASMCs) stimulated by TGF-β. By monitoring the changes of critical regulators in the presence of the formula, it was observed that the expression levels of TGF-β, IL-1β, Bcl-2, RhoA, ROCK1/2, ERK1/2, Snail, and Slug were markedly suppressed, whereas Bax expression exhibited the opposite effect. Compared with a well-characterized RhoA pathway inhibitor, Fasudil, SQHT generated equivalent or even higher inhibitory effects on these processes in ASMCs.

Conclusions: Collectively, these suggested that SQHT can reduce airway inflammation by inhibiting TGF-β-stimulated signaling pathways in ASMCs. These findings may provide a novel remedy for treating ASMC inflammation, which causes thickening and obstruction of the airway in chronic obstructive pulmonary disease.
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http://dx.doi.org/10.19852/j.cnki.jtcm.20220519.001DOI Listing
August 2022

Placental Abnormalities and Placenta-Related Complications Following Fertilization: Based on National Hospitalized Data in China.

Front Endocrinol (Lausanne) 2022 30;13:924070. Epub 2022 Jun 30.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Introduction: Emerging evidence has shown that fertilization (IVF) is associated with higher risks of certain placental abnormalities or complications, such as placental abruption, preeclampsia, and preterm birth. However, there is a lack of large population-based analysis focusing on placental abnormalities or complications following IVF treatment. This study aimed to estimate the absolute risk of placental abnormalities or complications during IVF-conceived pregnancy.

Methods: We conducted a retrospective cohort study of 16 535 852 singleton pregnancies with delivery outcomes in China between 2013 and 2018, based on the Hospital Quality Monitoring System databases. Main outcomes included placental abnormalities (placenta previa, placental abruption, placenta accrete, and abnormal morphology of placenta) and placenta-related complications (gestational hypertension, preeclampsia, eclampsia, preterm birth, fetal distress, and fetal growth restriction (FGR)). Poisson regression modeling with restricted cubic splines of exact maternal age was used to estimate the absolute risk in both the IVF and non-IVF groups.

Results: The IVF group (n = 183 059) was more likely than the non-IVF group (n = 16 352 793) to present placenta previa (aRR: 1.87 [1.83-1.91]), placental abruption (aRR: 1.16 [1.11-1.21]), placenta accrete (aRR: 2.00 [1.96-2.04]), abnormal morphology of placenta (aRR: 2.12 [2.07 to 2.16]), gestational hypertension (aRR: 1.55 [1.51-1.59]), preeclampsia (aRR: 1.54 [1.51-1.57]), preterm birth (aRR: 1.48 [1.46-1.51]), fetal distress (aRR: 1.39 [1.37-1.42]), and FGR (aRR: 1.36 [1.30-1.42]), but no significant difference in eclampsia (aRR: 0.91 [0.80-1.04]) was found. The absolute risk of each outcome with increasing maternal age in both the IVF and non-IVF group presented two patterns: an upward curve showing in placenta previa, placenta accreta, abnormal morphology of placenta, and gestational hypertension; and a J-shape curve showing in placental abruption, preeclampsia, eclampsia, preterm birth, fetal distress, and FGR.

Conclusion: IVF is an independent risk factor for placental abnormalities and placental-related complications, and the risk is associated with maternal age. Further research is needed to evaluate the long-term placenta-related chronic diseases of IVF patients and their offspring.
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http://dx.doi.org/10.3389/fendo.2022.924070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279699PMC
June 2022

Theoretical insights into the electroreduction of nitrate to ammonia on graphene-based single-atom catalysts.

Nanoscale 2022 Aug 4;14(30):10862-10872. Epub 2022 Aug 4.

Key Laboratory for Special Functional Materials of Ministry of Education, and School of Materials Science and Engineering, Henan University, Kaifeng 475004, China.

Electrocatalytic reduction of harmful nitrate (NO) to valuable ammonia (eNORR) is critical and attractive for both environmental remediation and energy transformation. A single atom catalyst (SAC) based on graphene represents one of the most promising eNORR catalysts. However, the underlying catalytic mechanism and the intrinsic factors dictating the catalytic activity trend remain unclear. Herein, using first-principles calculations, eNORR on TMN and TMN (TM = Ti-Ni) doped graphene was thoroughly investigated. Our results reveal that FeN doped graphene exhibits excellent eNORR performance with a low limiting potential of -0.38 V, agreeing with the experimental finding, which can be ascribed to the effective adsorption and activation of NO the charge "acceptance-donation" mechanism and its moderate binding due to the occupation of the d-p antibonding orbital. In particular, we found that eNORR activities are well correlated with the intrinsic properties of TM centers and their local environments. With the established activity descriptor, several other graphene-based SACs were efficiently screened out with excellent eNORR performance. Our studies could not only provide an atomic insight into the catalytic mechanism and activity origin of eNORR on graphene-based SACs, but also open an avenue for the rational design of SACs for eNORR towards ammonia by regulating the metal center and its local coordination environment.
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http://dx.doi.org/10.1039/d2nr02813aDOI Listing
August 2022

Genome-Wide Dissection of the Genetic Basis for Drought Tolerance in L. Races.

Front Plant Sci 2022 28;13:876095. Epub 2022 Jun 28.

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.

Drought seriously threats the growth and development of L. To dissect the genetic basis for drought tolerance in the L. germplasm, a population, consisting of 188 accessions of races and a cultivar (TM-1), was genotyped using the Cotton80KSNP biochip, and 51,268 high-quality single-nucleotide polymorphisms (SNPs) were obtained. Based on the phenotypic data of eight drought relative traits from four environments, we carried out association mapping with five models using GAPIT software. In total, thirty-six SNPs were detected significantly associated at least in two environments or two models. Among these SNPs, 8 and 28 (including 24 SNPs in 5 peak regions) were distributed in the A and D subgenome, respectively; eight SNPs were found to be distributed within separate genes. An SNP, TM73079, located on chromosome D10, was simultaneously associated with leaf fresh weight, leaf wilted weight, and leaf dry weight. Another nine SNPs, TM47696, TM33865, TM40383, TM10267, TM59672, TM59675, TM59677, TM72359, and TM72361, on chromosomes A13, A10, A12, A5, D6, and D9, were localized within or near previously reported quantitative trait loci for drought tolerance. Moreover, 520 genes located 200 kb up- and down-stream of 36 SNPs were obtained and analyzed based on gene annotation and transcriptome sequencing. The results showed that three candidate genes, , and , may play important roles in drought tolerance. The current GWAS represents the first investigation into mapping QTL for drought tolerance in races and provides important information for improving cotton cultivars.
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http://dx.doi.org/10.3389/fpls.2022.876095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274165PMC
June 2022
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