Publications by authors named "Yuanyuan Li"

1,814 Publications

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Imputation of the major histocompatibility complex region identifies major independent variants associated with bullous pemphigoid and dermatomyositis in Han Chinese.

J Dermatol 2022 Jun 25. Epub 2022 Jun 25.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

As autoimmune skin diseases, both bullous pemphigoid (BP) and dermatomyositis (DM) show significant associations with the major histocompatibility complex (MHC) region. In fact, the coexistence of BP and DM has been previously reported. Therefore, we hypothesized that there may be a potential genetic correlation between BP and DM. Based on data for 312 BP patients, 128 DM patients, and 6793 healthy control subjects, in the MHC region, we imputed single-nucleotide polymorphisms (SNP), insertions and deletions (INDEL), and copy number variations (CNV) using the 1KGP phase 3 dataset and amino acids (AA) and SNP using a Han-MHC reference database. An association study revealed the most significant SNP associated with BP, namely, rs580921 (p = 1.06E-08, odds ratio [OR] = 1.61, 95% confidence interval [CI] = 1.37-1.90), which is located in the C6orf10 gene, and the most significant classic human leukocyte antigen (HLA) allele associated with DM, namely, HLA-DPB1*1701 (p = 6.56E-10, OR = 3.61, 95% CI = 2.40-5.42). Further stepwise regression analyses with rs580921 identified a threonine at position 163 of the HLA-B gene as a new independent disease-associated AA, and HLA-DPB1*1701 indicated that no loci were significant. Three-dimensional ribbon models revealed that the HLA-B AA position 163 (p = 3.93E-07, OR = 1.64, 95% CI = 1.35-1.98) located in the α2 domain of the HLA-B molecule was involved in the process of specific antigen presentation. The calculations showed that there was no significant genetic correlation between BP and DM. Our study identified three significant loci in the MHC region, proving that the HLA region was significantly correlated with BP and DM separately. Our research highlights the key role of the MHC region in disease susceptibility.
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http://dx.doi.org/10.1111/1346-8138.16499DOI Listing
June 2022

Isolation, Molecular Characterization, and Antimicrobial Resistance of Selected Culturable Bacteria From Crayfish ().

Front Microbiol 2022 7;13:911777. Epub 2022 Jun 7.

Department of Medical Microbiology, School of Biology and Basic Medical Science, Medical College of Soochow University, Suzhou, China.

Red swamp crayfish () have become one of the favorite aquatic products in China. The modern farming mode which uses antibiotics to prevent diseases could impact the bacteria in crayfish intestines. Here, we determined the distribution and antimicrobial resistance phenotypes of the selected culturable bacteria in crayfish intestines and characterized an isolate with last-line antibiotic resistance determinant. Totally, 257 strains were isolated from 115 crayfish. These strains were highly diverse, with sp. ( = 94, 36.6%) and sp. ( = 88, 34.2%) being dominant. Other isolates belonged to genera , , , , , , , and . Most strains from crayfish were susceptible to all tested antibiotics. None of the isolates carried last-line antibiotic resistance genes except one isolate with was detected, which is the first report of -positive isolate from red swamp crayfish. Whole-genome sequencing suggested it belonged to ST48 and carried several resistance genes. was located within an Tn-like transposon linked to an external 5 bp sequence (ACTAT) on both sides on a IncHI1B/HI1A/FIA multi-replicon plasmid. This transposon was considered to be acquired by replicative transposition mediated by IS. The emergence of bacteria with last-line antibiotic resistance genes in crayfish poses serious threat to public health since crayfish could act as a reservoir for the transfer of resistance to humans.
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http://dx.doi.org/10.3389/fmicb.2022.911777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209738PMC
June 2022

Fluorescent Immunoassay with a Copper Polymer as the Signal Label for Catalytic Oxidation of -Phenylenediamine.

Molecules 2022 Jun 8;27(12). Epub 2022 Jun 8.

Henan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang Normal University, Anyang 455000, China.

This work suggested that Cu ion coordinated by the peptide with a histidine (His or H) residue in the first position from the free N-terminal reveals oxidase-mimicking activity. A biotinylated polymer was prepared by modifying His residues on the side chain amino groups of lysine residues (denoted as K) to chelate multiple Cu ions. The resulting biotin-poly-(K-Cu) polymer with multiple catalytic sites was employed as the signal label for immunoassay. Prostate specific antigen (PSA) was determined as the model target. The captured biotin-poly-(K-Cu) polymer could catalyze the oxidation of -phenylenediamine (OPD) to produce fluorescent 2,3-diaminophenazine (OPDox). The signal was proportional to PSA concentration from 0.01 to 2 ng/mL, and the detection limit was found to be eight pg/mL. The high sensitivity of the method enabled the assays of PSA in real serum samples. The work should be valuable for the design of novel biosensors for clinical diagnosis.
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http://dx.doi.org/10.3390/molecules27123675DOI Listing
June 2022

A Bioink Derived From Human Placenta Supporting Angiogenesis.

Biomed Mater 2022 Jun 22. Epub 2022 Jun 22.

Institute of Zoology Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District District, Beijing, 100101, CHINA.

Bioprinting is an emerging approach for constructing sophisticated tissue analogues with detailed architectures such as vascular networks, which requires bioink fulfill the highly printable property and provide a cell-friendly microenvironment mimicking native extracellular matrix (ECM). Here, we developed a human placental ECM-derived bioink (hp-bioink) meeting the requirements of 3D printing for printability and bioactivity. We first decellularized the human placenta, followed by enzymatic digestion, dialysis, lyophilization, and re-solubilization to convert the extracts into hp-bioink. Then, we demonstrated that 3%-5% of hp-bioink can be printed with self-standing and 1%-2% of hp-bioink can be embedded with suspended hydrogels. Moreover, hp-bioink supports HUVEC assembly in vitro and angiogenesis in mice in vivo. Our research enriched the bank of human-derived bioink, and provided a new opportunity to further accelerate bioprinting research and application.
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http://dx.doi.org/10.1088/1748-605X/ac7b5bDOI Listing
June 2022

Solid-Liquid State Transformable Magnetorheological Millirobot.

ACS Appl Mater Interfaces 2022 Jun 21. Epub 2022 Jun 21.

Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, P. R. China.

Magnetically actuated soft millirobots (magneto-robot) capable of accomplishing on-demand tasks in a remote-control manner using noninvasive magnetic fields are of great interest in biomedical settings. However, the solid magneto-robots are usually restricted by the limited deformability due to the predesigned shape, while the liquid magneto-robots are capable of in situ shape reconfiguration but limited by the low stiffness and geometric instability due to the fluidity. Herein, we propose a magneto-active solid-liquid state transformable millirobot (named MRF-Robot) made from a magnetorheological fluid (MRF). The MRF-Robot can transform freely and rapidly between the Newtonian fluid in the liquid state upon a weak magnetic field (∼0 mT) and the Bingham plasticity in the solid state upon a strong magnetic field (∼100 mT). The MRF-Robot in the liquid state can realize diverse behaviors of large deformation, smooth navigation, in situ splitting, merging, and gradient pulling actuated by a weak magnetic field with a high gradient. The MRF-Robot in the solid state is distinguished for the controllable locomotion with reconfigured shapes and versatile object manipulations (including pull, push, and rotate the objects) driven by a strong magnetic field with a high gradient. Moreover, the MRF-Robot could continuously maneuver to accomplish diverse tasks in the comprehensive scenes and achieve liquid-drug delivery, thrombus clearance, and fluid-flow blockage in the phantom vascular model under magnetic actuation.
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http://dx.doi.org/10.1021/acsami.2c05251DOI Listing
June 2022

Solidification, remediation and long-term stability of heavy metal contaminated soil under the background of sustainable development.

Sci Rep 2022 Jun 20;12(1):10330. Epub 2022 Jun 20.

Department of Civil and Architectural Engineering, Qingdao University of Technology (School of Linyi), Linyi, 273400, Shandong, China.

At present, the global pollution has seriously exceeded the standard. With the passage of time, pollution has gradually affected people's daily lives, but the solution to pollution is far from achieving a better treatment effect. For the treatment of pollution, in addition to considering the treatment effect, it is also necessary to consider whether the treatment method will cause pollution and the cost of the treatment of the pollutants. As one of the lifelines of human survival, the land is also suffering from pollution. The impact of heavy metal pollution is particularly serious, and there is no better solution. Based on this, this paper proposes a curing agent based on sustainable remediation to solve the soil pollution of heavy metals. The main material is Basic oxygen furnace slag (BOFS), which has excellent social development characteristics in all aspects, and the raw materials are calcium carbide residue (CCR) and phosphogypsum (PG) to explore a more suitable curing agent. (consisting of BOFS, CCR, and PG, abbreviated as BCP). The experimental results in this paper show that the volume of pores and pores in the agglomerates are slightly reduced, and the content of curing agent is increased from 4 to 10%, while the corresponding volume is only reduced by 0.006 and 0.017 mL/g. Therefore, it can be seen that the reduction of the pore volume between the aggregates of the stabilized species of BCP has made a major contribution to the strength development.
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http://dx.doi.org/10.1038/s41598-022-14122-zDOI Listing
June 2022

Transcriptome Analysis to Identify Crucial Genes for Reinforcing Flavins-Mediated Extracellular Electron Transfer in .

Front Microbiol 2022 1;13:852527. Epub 2022 Jun 1.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.

Flavins serve as the electron mediators in , determining the extracellular electron transfer (EET) rate. Currently, metabolic engineering of flavins biosynthetic pathway has been studied for improving EET. However, the cellular response triggered by flavins that contribute to EET remains to be elucidated. In this study, the riboflavin-overproducing strain C5 (expressing the flavins synthetic genes in plasmid PYYDT) and the PYYDT strain (harboring the empty plasmid PYYDT) in the microbial fuel cells are applied for comparative transcriptomic analyses to investigate beneficial gene targets that could improve EET. From the differentially expressed genes, we select the significantly upregulated and downregulated genes for inverse engineering in . The results show that overexpression of and , and inactivation of , , and are able to improve the EET capability. Combinatorial modulation of these five genes results in the recombinant strain CM4, achieving the maximum power density of 651.78 ± 124.60 mW/m, 1.97 folds of the parental strain. These genes modulation is speculated to reduce the ROS damage and to promote cytochrome synthesis and heme accumulation, which coherently enhance EET. Our findings facilitate in-depth understanding of the mechanism of flavins-mediated EET and provide new insights in promoting EET of for electricity generation.
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http://dx.doi.org/10.3389/fmicb.2022.852527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198578PMC
June 2022

Single-Cell RNA-Sequencing Reveals the Cellular and Genetic Heterogeneity of Skin Scar to Verify the Therapeutic Effects and Mechanism of Action of Dispel-Scar Ointment in Hypertrophic Scar Inhibition.

Evid Based Complement Alternat Med 2022 8;2022:7331164. Epub 2022 Jun 8.

The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang, China.

Hypertrophic scarring (HS), caused by excessive fibrosis of injured skin, imposes a psychological burden and creates a source of distress that impairs the quality of life of affected individuals. However, the gold standard for HS treatment has not yet been determined due to the complicated and difficult nature of the routines and procedures involved. Previous studies have indicated that the topical application of certain active components found in traditional Chinese medicines shows potential as a therapeutic alternative for scars. Here, single-cell RNA-sequencing was performed to determine cellular heterogeneity and identify marker genes and mechanisms associated with HS. It was found that fibroblasts comprise the largest proportion of HS cell types. The marker genes that were highly expressed in fibroblasts were extracellular matrix (ECM)-related, whereas ECM-receptor interactions and the transforming growth factor (TGF)- signalling pathway were also found to be active. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, which was applied to identify the molecular compounds of Dispel-Scar Ointment (DSO), revealed 74 effective chemical components belonging to 14 types of constituents, such as flavonoids, tanshinones, salvianolic acids, glycosides, and phthalides. Furthermore, studies using rat scar models showed that the topical application of , , peach kernel, safflower, and motherwort exerted beneficial effects on fibroblasts. DSO promoted scar maturation and reduced scar areas, its efficacy being similar to that of topically applied silicone. Functional studies using immunofluorescence staining, western blotting, and quantitative real-time polymerase chain reaction demonstrated that DSO may target the TGF-/Smad pathway to inhibit collagen synthesis and promote ECM remodelling. However, further mechanistic research and single-drug prescription studies may be required to identify the specific effective compound or active ingredient of DSO, which would provide more substantial evidence regarding the potential therapeutic value of traditional herbs in HS.
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http://dx.doi.org/10.1155/2022/7331164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200508PMC
June 2022

Altered Effective Connectivity of Resting-State Networks by Tai Chi Chuan in Chronic Fatigue Syndrome Patients: A Multivariate Granger Causality Study.

Front Neurol 2022 3;13:858833. Epub 2022 Jun 3.

Department of Rehabilitation, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Numerous evidence has shown that patients with chronic fatigue syndrome (CFS) have changes in resting brain functional connectivity, but there is no study on the brain network effect of Tai Chi Chuan intervention in CFS. To explore the influence of Tai Chi Chuan exercise on the causal relationship between brain functional networks in patients with CFS, 21 patients with CFS and 19 healthy controls were recruited for resting-state functional magnetic resonance imaging (rs-fMRI) scanning and 36-item Short-Form Health Survey (SF-36) scale assessment before and after 1month-long training in Tai Chi Chuan. We extracted the resting brain networks using the independent component analysis (ICA) method, analyzed the changes of FC in these networks, conducted Granger causality analysis (GCA) on it, and analyzed the correlation between the difference causality value and the SF-36 scale. Compared to the healthy control group, the SF-36 scale scores of patients with CFS were lower at baseline. Meanwhile, the causal relationship between sensorimotor network (SMN) and default mode network (DMN) was weakened. The above abnormalities could be improved by Tai Chi Chuan training for 1 month. In addition, the correlation analyses showed that the causal relationship between SMN and DMN was positively correlated with the scores of Role Physical (RP) and Bodily Pain (BP) in CFS patients, and the change of causal relationship between SMN and DMN before and after training was positively correlated with the change of BP score. The findings suggest that Tai Chi Chuan is helpful to improve the quality of life for patients with CFS. The change of Granger causality between SMN and DMN may be a readout parameter of CFS. Tai Chi Chuan may promote the functional plasticity of brain networks in patients with CFS by regulating the information transmission between them.
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http://dx.doi.org/10.3389/fneur.2022.858833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203735PMC
June 2022

Bifunctional Solid-State Copolymer Electrolyte with Stabilized Interface for High Performance Lithium Metal Battery in a Wide Temperature Range.

ChemSusChem 2022 Jun 17. Epub 2022 Jun 17.

Zhejiang University, College of Chemical and Biological Engineering, 38# Zheda Road, 310027, Hangzhou, CHINA.

Solid-state polymer electrolytes (SPEs) are expected to guarantee safe and durable operations of lithium metal batteries (LMBs). Herein, inspired by the salutary poly(vinyl ethylene carbonate) (PVEC) component in solid electrolyte interface, cross-linking vinyl ethylene carbonate and ionic liquid copolymers are synthesized by in-situ polymerization to serve as polymer electrolyte for LMBs. On one hand, due to rich ester bonds of PVEC, Li + can transfer by coupling/decoupling with oxygen atoms. On the other hand, the imidazole ring of ionic liquid can facilitate the dissociation of lithium salt to promote the free movement of Li + . The bifunctional component synergistically increases the ionic conductivity of the SPE to 1.97×10 -4 S cm -1 at 25°C. Meanwhile, it also shows a wide electrochemical window, superior mechanical properties, outstanding non-combustibility, and excellent interfacial compatibility. The bifunctional copolymer based LiFePO 4 batteries can normally operate at 0 to 60°C, which is a hopeful candidate for wide temperature LMBs.
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http://dx.doi.org/10.1002/cssc.202200993DOI Listing
June 2022

Development of Biocompatible Ene-Ligation Enabled by Prenyl-Based β-Caryophyllene with Triazoline/Selectfluor under Physiological Conditions.

J Org Chem 2022 Jun 16. Epub 2022 Jun 16.

The Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Here, we first report a rapid and highly selective biocompatible ligation that proceeds via a strain-promoted prenyl-involved [2, 3]-Ene rearrangement process. We demonstrate the usefulness of naturally occurring strain-promoted β-caryophyllene with triazoline (PTAD)/Selectfluor in the study of tagging molecule-of-interest. Experiments in peptide (Histone H3 (1-21) and Myhc (614-629)) and protein (BSA, βLG, and HSP40) models exemplified the utility of the Ene-ligation for in vivo imaging and tracking.
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http://dx.doi.org/10.1021/acs.joc.2c00841DOI Listing
June 2022

3D printed controllable microporous scaffolds support embryonic development in vitro.

J Cell Physiol 2022 Jun 14. Epub 2022 Jun 14.

The State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the "black box". 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo-scaffold attachments. T-positive cells and FOXA2-positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool.
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http://dx.doi.org/10.1002/jcp.30810DOI Listing
June 2022

Establishing a core outcome set for neurogenic bladder trials: study protocol for a scoping review and Delphi surveys.

Trials 2022 Jun 13;23(1):485. Epub 2022 Jun 13.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, No.199, Donggang West Road, Lanzhou City, 730000, Gansu Province, China.

Background: Neurogenic bladder (NGB) is a chronic and disabling condition with a high prevalence rate, which can cause economic burden on patients and their families and reduce the quality of life of patients. Researchers have carried out a large number of clinical trials on the effectiveness and safety of different interventions for the treatment of NGB. The published clinical trials of NGB generally suffered from inconsistent and irregular reporting of outcome indicators. To facilitate future research studies of NGB, a core outcome set (COS) is required, which helps translate the results into high-quality evidence.

Methods And Analysis: This mixed-method project has four phases instrument: in phase 1, a scoping review of the literature to identify outcomes that have been reported in clinical trials and systematic reviews of clinical trials of interventions for NGB; in phase 2, a qualitative component using interviews to obtain the views of NGB patients, families, and their caregivers; in phase 3, Delphi survey among stakeholders to prioritize the core outcomes; and in phase 4, a face-to-face consensus meeting to discuss and agree on the final NBG COS.

Conclusions: We will develop a COS that should be reported in future clinical trials of NGB.

Trial Registration: Core Outcome Measures in Effectiveness Trials (COMET) Initiative database registration: http://www.comet-initiative.org/studies/details/1985 . Registered on 02 January 2022. INPLASY  INPLASY202210007.
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http://dx.doi.org/10.1186/s13063-022-06419-1DOI Listing
June 2022

Electroacupuncture Ameliorates Mechanical Allodynia of a Rat Model of CRPS-I via Suppressing NLRP3 Inflammasome Activation in Spinal Cord Dorsal Horn Neurons.

Front Cell Neurosci 2022 25;16:826777. Epub 2022 May 25.

Key Laboratory of Acupuncture and Neurology of Zhejiang Province, Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.

Complex regional pain syndrome type-I (CRPS-I) is a chronic neurological disorder that results in severe pain and affects patients' life quality. Conventional therapies usually lack effectiveness. Electroacupuncture (EA) is an effective physical therapy for relieving CRPS-I pain. However, the mechanism underlying EA-induced analgesia on CRPS-I still remain unknown. Spinal NLRP3 inflammasome was recently identified to contribute to pain and neuroinflammation in a rat model of CRPS-I by our group. Here, we aimed to study whether EA could inhibit spinal NLRP3 inflammasome activation, thus resulting in pain relief and attenuation of spinal neuroinflammation in the rat model of CRPS-I. We established the rat chronic post-ischemic pain (CPIP) model to mimic CRPS-I. CPIP rats developed remarkable mechanical allodynia that could be relieved by daily EA intervention. NLRP3 inflammasome was activated in spinal cord dorsal horn (SCDH) of CPIP rats, accompanied with over-production of pro-inflammatory cytokine IL-1β. Immunostaining revealed that the cellular distribution of NLRP3 was predominantly located in SCDH neurons. Pharmacological activation of NLRP3 inflammasome is sufficient to produce persistent mechanical allodynia in naïve animals, whereas blocking NLRP3 inflammasome attenuates mechanical allodynia of CPIP rats. EA exclusively reduced NLRP3 overexpression in SCDH neurons and attenuated spinal glial cell over-activation in CPIP rats. EA-induced anti-allodynia with attenuation of spinal glial cell over-activation were all mimicked by intrathecal blocking NLRP3 inflammasome and reversed by activating NLRP3 inflammasome, respectively, through pharmacological methods. Finally, spinal blocking IL-1β attenuated mechanical allodynia and spinal glial cell over-activation in CPIP rats, resembling the effects of EA. In all, these results demonstrate that spinal NLRP3 inflammasome activation contributes to mechanical allodynia of the rat model of CRPS-I and EA ameliorates mechanical allodynia through inhibiting NLRP3 inflammasome activation in SCDH neurons. Our study further supports EA can be used as an effective treatment for CRPS-I.
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http://dx.doi.org/10.3389/fncel.2022.826777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174662PMC
May 2022

scarless skin healing of a severe human burn wound induced by a hCTLA4Ig gene-transferred porcine skin graft.

Int J Med Sci 2022 21;19(5):952-964. Epub 2022 May 21.

Department of Burns and Plastic Surgery, the First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, P.R. China.

Preventing fibrosis or hypertrophic scar formation following tissue damage is still a big challenge despite the numerous approaches clinicians currently use. Hitherto, no written account was available of a successful case of scarless skin healing after a severe burn injury. Here, we report the first case of the "perfect regenerative healing" of a severe burn wound with no hypertrophic scar formation in which a postage stamp skin autograft was covered with human cytotoxic-T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) gene-transferred pig skin. We also discuss the mechanisms involved in the scarless healing of human burn wounds.
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http://dx.doi.org/10.7150/ijms.62438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149639PMC
June 2022

Convolutional neural networks-based health risk modelling of some heavy metals in a soil-rice system.

Sci Total Environ 2022 Sep 9;838(Pt 4):156466. Epub 2022 Jun 9.

College of Resources and Environment, Hunan Agricultural University, Changsha 410128, PR China.

The long-term consumption of heavy metal-rich rice can cause serious harm to human health. However, the existing health risk assessment (HRA) can only be performed after the rice has been harvested, and this approach belongs to a passive and lagging pattern. This study is the first to explore the feasibility of health risk (HR) prediction by proposing the indirect model CNNHR-IND and the direct model CNNHR-DIR based on the convolutional neural network (CNN) technology. The dataset included 390 pairs of soil-rice samples collected from You County, China, with 17 environmental covariates. The R values for CNNHR-IND for non-carcinogenic and carcinogenic risks were 0.578 and 0.554, respectively, and those for CNNHR-DIR were 0.647 and 0.574, respectively. The results demonstrated that both models performed well, especially CNNHR-DIR had a higher estimation accuracy. The spatial autocorrelation analysis indicated that CNNHR-DIR exerted no systematic bias in the prediction results for health risks, confirming the rationality of the CNNHR-DIR model. The sensitivity analysis further confirmed the generalizability and robustness of CNNHR-DIR. This study proved the feasibility of HR prediction and the potential of CNN technology in HRA, and is significant regarding early risk warnings of rice planting and the sustainable development of public health.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156466DOI Listing
September 2022

Psychosocial resources moderate the association between stress and insomnia among patients with digestive system cancers.

Psychol Health Med 2022 Jun 9:1-8. Epub 2022 Jun 9.

Department of Health Psychology, School of Nursing and Rehabilitation, Cheeloo College of Medicine, Shandong University, Jinan, P.R. China.

This study explored the profiles of psychosocial resources combined with resilience and family care, and analyzed their moderating effects on the relationship between stress and insomnia in patients with digestive system cancers. A total of 366 patients were recruited to participate in this study from two tertiary hospitals in China. They were assessed using the Perceived Stress Scale - 4 items, Insomnia Severity Index, Family Concern Index Questionnaire, and 10-item Connor-Davidson Resilience Scale. Latent profile analysis and the BCH (Bolck, Croon & Hagenaars) method were used to identify the subtypes and estimate the moderating role of psychosocial coping resources. About 62.3% of participants had insomnia symptoms. Insomnia was positively correlated to the stress ( = 0.25, < 0.001). The latent classes were the low resources class (32.8%), the medium resources class (46.1%), and high resources class (21.1%). Among these, in low (estimate value = 0.563, = 0.003) and medium (estimate value = 0.301, = 0.029) resources class, stress had an effect on insomnia. There was no association between stress and insomnia in high resources class (estimate value = 0.165, = 0.637). Stress might be associated with to insomnia problems, whereas patients with high psychosocial resources are more not vulnerable. Interventions to improve family function and resilience could contribute to easing the insomnia of patients with digestive system cancers.
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http://dx.doi.org/10.1080/13548506.2022.2085877DOI Listing
June 2022

Solar-Driven Soil Remediation along with the Generation of Water Vapor and Electricity.

Nanomaterials (Basel) 2022 May 25;12(11). Epub 2022 May 25.

Key Laboratory of Cluster Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

As a renewable energy source, solar energy has become an important part of human energy use. However, facilities utilizing solar energy are often complex and technically difficult, and preparation equipment and materials are expensive, while these equipment and materials often cause new environmental pollution. Soil, which exists in large quantities on the earth's surface, is an inexhaustible natural material with loose and stable properties. Due to the specificity of its composition and microscopic form, the soil has an inherent advantage as a medium for solar thermal and photovoltaic conversion. Here, we built an integrated solar energy utilization system, the Integrated Soil Utilization Module (ISUM), integrating multi-functions into one hybrid system, which enables solar-driven water vapor and electricity generation and soil remediation. The evaporation rate of the soil represented by the rocky land was 1.2 kg·m·h under 1-sun irradiation with evaporation induced voltage of 0.3 V. With only seven days of continuous exposure to sunlight, the removal of heavy metal ions from the soil reached 90%, while the pH was raised to near neutral. The combined application of readily available natural soil with solar energy not only demonstrates the potential of a soil for solar desalination and power generation, but in addition, solar-driven interfacial evaporation provides an energy-efficient, environmentally friendly, and sustainable method for purifying heavy metal and acid-contaminated soil.
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http://dx.doi.org/10.3390/nano12111800DOI Listing
May 2022

The Impact of Social Entrepreneurship, Corporate Social Responsibilities, and Working Capital Management Practices on the Performance of Tourism Small-Medium Enterprises (SMEs) During COVID-19: Moderating Role of Employee Behavior.

Front Psychol 2022 23;13:869856. Epub 2022 May 23.

Department of Science, Universidad Nacional Santiago Antunez de Mayolo, Huaraz, Peru.

The failure or success of an enterprise depends upon its working capital management practices (WCMP) along with effective corporate social responsibilities (CSR) and social entrepreneurship (SE). These factors ensure not only the soundness of financial indicators but also the profitability of an enterprise. Therefore, the objective of this study was to determine the impact of CSR, SE, and WCMP on the performance of small-medium enterprises (SMEs) of tourism sector, during the lockdown period in Malaysia. The goals also include the investigation of moderating role of employee behavior (EB) among the nexus of CSR, SE, WCMP, and performance of SMEs. The survey was performed to find out the practices adopted by the SMEs during corona days. A quantitative research method has been adopted to get data with convenient sampling technique, and PLS-SEM has been exploited to find out the significant nexus among CSR, SE, WCMP, and the performance of SMEs. The results indicate that CSR, SE, and WCMP have a positive association with the performance of SMEs in Malaysia. The results also indicate that EB positively moderates among the nexus of CSR, SE, WCMP, and the performance of SMEs in Malaysia. Stock review strategies by the SMEs during the corona days also exhibit significant differences except for no stock review. Significant differences between the enterprises show a lack of adaptation of the financial indicators that determine enterprise soundness. The study not only provides guidelines for entrepreneurs of SMEs but also helps in maintaining standards for the evaluation of the enterprises.
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http://dx.doi.org/10.3389/fpsyg.2022.869856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168768PMC
May 2022

High-throughput sequencing reveals rhizosphere fungal community composition and diversity at different growth stages of in the lower reaches of the Tarim River.

PeerJ 2022 2;10:e13552. Epub 2022 Jun 2.

Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China.

Background: is one of the most ancient and primitive tree species of and plays an important role in maintaining the ecological balance in desert areas. To decipher the diversity, community structure, and relationship between rhizosphere fungi and environmental factors at different growth stages of demands an in-depth investigation.

Methods: In this study, at different growth stages (young, medium, overripe, and decline periods) was selected as the research object, based on the determination of the physicochemical properties of its rhizosphere soil, the fungal community structure and diversity of and their correlation with soil physicochemical properties were comprehensively analyzed through high-throughput sequencing technology (internal transcribed spacer (ITS)) and bioinformatics analysis methods.

Results: According to the analysis of OTU annotation results, the rhizosphere soil fungal communities identified in were categorized into10 phyla, 36 classes, 77 orders, 165 families, 275 genera and 353 species. The alpha diversity analysis showed that there was no obvious change between the different growth stages, while beta diversity analysis showed that there were significantly differences in the composition of rhizosphere soil fungal communities between mature and overripe trees ( = 0.31, = 0.001), mature and deadwood ( = 0.28, = 0.001). Ascomycota and Basidiomycota were dominant phyla in the rhizosphere fungal community and the dominant genera were and The relative abundance of the top ten fungi at each classification level differed greatly in different stages. Canonical correspondence analysis (CCA) and Spearman's correlation analysis showed that conductivity (EC) was the main soil factor affecting the composition of rhizosphere soil fungal community ( < 0.01), followed by total dissolvable salts (TDS) and available potassium (AK) ( < 0.05).

Conclusions: Our data revealed that the rhizosphere fungal communities at the different growth stages of have differences, conductivity (EC) was the key factor driving rhizosphere fungi diversity and community structure, followed by total dissolvable salts (TDS) and available potassium (AK).
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http://dx.doi.org/10.7717/peerj.13552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167583PMC
June 2022

Efficacy and safety of piezocision in accelerating maxillary anterior teeth en-masse retraction: study protocol for a randomized controlled trial.

Trials 2022 Jun 7;23(1):476. Epub 2022 Jun 7.

Department of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China.

Background: Orthodontic treatment is commonly more time-consuming in adults than in teenagers, especially when it comes to the maxillary en-masse retraction, which may take 9 months or even longer. As to solve this concern, orthodontists have been striving to seek new methods for shortening orthodontic treatment time. Piezocision, as a popular alternative treatment, has been widely used in different types of tooth movement. However, its effect on en-masse retraction of maxillary anterior teeth remains unclear. This randomized controlled trial intends to figure out the role piezocision plays in accelerating en-masse retraction.

Methods: This protocol is designed for a prospective, single-center, assessor-blinded and parallel-group randomized controlled trial. Twenty adult patients aged from 18 to 40 whose orthodontic treatment required bilateral maxillary first premolars extraction will be randomly assigned to the piezocision group and the control group at a ratio of 1:1. The piezocision group will undergo en-masse retraction immediately after the piezo surgery, while the control group will start en-masse retraction directly. Both groups will be followed up every 2 weeks to maintain the retraction force until the end of space closure. The space closing time is set as the primary endpoint. Meanwhile, the secondary endpoints include the change of root length, labial and palatal alveolar bone thickness, vertical bone height, probing depth of maxillary anterior teeth, cephalometric measurements, visual analogue scale, and postoperative satisfaction questionnaire.

Discussion: This study will attempt to provide more convincing evidence to verify whether piezocision will shorten the time of en-masse retraction or not. Distinguished with previous studies, our study has made some innovations in orthodontic procedure and primary outcome measurement, aiming to clarify the efficacy and safety of piezocision-assisted en-masse retraction in Chinese population.

Trial Registration: Chinese Clinical Trial Registry ChiCTR 1900024297 . Registered on 5 July 2019.
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http://dx.doi.org/10.1186/s13063-022-06389-4DOI Listing
June 2022

Transcriptomic signatures associated with autoimmune thyroiditis in papillary thyroid carcinoma and cancer immunotherapy-induced thyroid dysfunction.

Comput Struct Biotechnol J 2022 16;20:2391-2401. Epub 2022 May 16.

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Up to 20% of patients treated with anti-PD-1/PD-L1 inhibitors suffered from thyroid dysfunctions, yet the mediators associated with their occurrence remain unclear. The increasing coincidence of papillary thyroid carcinoma (PTC) with Hashimoto thyroiditis (HT) and the high vulnerability of thyroid to immunotherapy motivated us to discover the similarities and their underlying transcriptomic basis. Clinical characteristics analysis of 468 PTC patients from two independent cohorts and meta-analysis of 22,155 PTC patients unveiled a strong negative association between HT and recurrence in PTC patients. Transcriptome analysis of both cohorts showed PTC patients with HT were enriched in macrophages, CD8 and CD4 cytotoxic T cells, which was further validated by single-cell transcriptome analysis of 17,438 cells from PTC patients, and CD8 T cells were correlated with disease-free survival of PTC patients. In both cohorts and single-cell dataset, elevated expression of PD-1-related genes was observed in the HT group, and appeared to be a target for enhancing the activation of CD8 T cells. Correlation analysis of 3,318 thyroid adverse events from 39,123 patients across 24 tumor types and molecular signatures demonstrated similar signatures associated with autoimmune thyroiditis in PTC and thyroid immune-related adverse events (irAEs), and several multi-omics signatures, including signatures of CD8A and CD8 T cells, showed positive associations with the odds ratio of thyroid irAEs. Our results unveil shared molecular signatures underlying thyroid dysfunction between patients receiving immunotherapies and PTC patients suffering from HT, which may shed light on managing the adverse events during cancer immunotherapy.
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http://dx.doi.org/10.1016/j.csbj.2022.05.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125670PMC
May 2022

Association between tooth loss and hypertension: A systematic review and meta-analysis.

J Dent 2022 Jun 2;123:104178. Epub 2022 Jun 2.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China; The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Hena 453003, China. Electronic address:

Objectives: Poor oral health status may increase the risk of cardiovascular disease. However, whether a specific association exists between tooth loss and hypertension is inconclusive. Accordingly, a quantitative systematic review was conducted to investigate the relationship between tooth loss and hypertension.

Methods: Systematic search, data analysis and quality assessment were conducted on relevant literature published in PubMed, Embase, Web of Science and Cochrane Libraries until October 2021. Odds ratio (OR) with 95% confidence interval (CI) was used as effect size to evaluate the association between tooth loss and hypertension. Meta-regression and subgroup analyses were performed to identify whether difference was associated with study-level factors.

Results: Of the 56 studies selected, 28 studies in 16 different countries involving 1,224,821 individuals were eligible for the systematic review. After adjustment for confounding factors, individuals with tooth loss had a higher risk for hypertension (OR 1.20; 95%CI 1.10-1.30, I = 40.02%). With respect to the risk of hypertension on tooth loss, individuals with hypertension still had a higher risk for tooth loss (OR 1.35; 95%CI 1.07-1.62, I = 51.10%). Age limitation of the included population is the major source of heterogeneity. However, studies in which the population was limited to the elderly did not report an increase in association between tooth loss and hypertension compared with studies without age limitation.

Conclusions: The results suggest a bidirectional association between tooth loss and hypertension. Future longitudinal prospective studies are required to establish causality between tooth loss and hypertension.

Clinical Significance: Subjects with severe tooth loss should be carefully monitored for the manifestation of hypertension. The oral health status of hypertensive patients should also be meticulous maintained to prevent unwarranted tooth loss.
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http://dx.doi.org/10.1016/j.jdent.2022.104178DOI Listing
June 2022

How climate change and land-use evolution relates to the non-point source pollution in a typical watershed of China.

Sci Total Environ 2022 Sep 31;839:156375. Epub 2022 May 31.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.

The water quality of Le 'an River Watershed (LRW) is crucial to the water environmental safety of Poyang Lake, especially the concentration of nitrogen and phosphorus. The effect of climate and land use change on watershed water quality has always been under the attention of local managers. More importantly, the lack of detailed studies on climate and land use impact on river water quality has prevented sustainable water security management in the LRW. Therefore, this study aimed to quantify the weight of climate and land use on nutrient loss in the LRW, respectively. We divided the historical period (1990-2020) into six scenarios and a baseline scenario. TN and TP losses in the watershed were simulated using Soil and Water Assessment Tool (SWAT), and the weight of climate and land use were quantified in overall, by period, and by region. The results showed that the weight of climate was greatly higher than land use with values around 90%. However, the weight of land use had a positive cumulative effect in a certain period, and its influence could not be neglected. The climate in all scenarios led to a reduction in nutrient loss, while land use was found to slightly increase the nutrient loss yield. In addition to, unique regional topographic features, urbanization rates, and climatic conditions could cause spatial heterogeneity in the climatic and land use weights.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156375DOI Listing
September 2022

Cold chain and severe acute respiratory syndrome coronavirus 2 transmission: a review for challenges and coping strategies.

Med Rev (Berl) 2022 Feb 1;2(1):50-65. Epub 2022 Mar 1.

Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Since June 2020, the re-emergence of coronavirus disease 2019 (COVID-19) epidemics in parts of China was linked to the cold chain, which attracted extensive attention and heated discussions from the public. According to the typical characteristics of these epidemics, we speculated a possible route of transmission from cold chain to human. A series of factors in the supply chain contributed to the epidemics if the cold chain were contaminated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), such as temperature, humidity, personal hygiene/protection, and disinfection. The workers who worked in the cold chain at the receiving end faced a higher risk of being infected when they were not well protected. Facing the difficult situation, China put forward targeted and powerful countermeasures to block the cold chain-related risk. However, in the context of the unstable pandemic situation globally, the risk of the cold chain needs to be recognized and evaluated seriously. Hence, in this review, we reviewed the cold chain-related epidemics in China, analyzed the possible mechanisms, introduced the Chinese experience, and suggested coping strategies for the global epidemic prevention and control.
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http://dx.doi.org/10.1515/mr-2021-0019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047647PMC
February 2022

Identification of cell types in multiplexed in situ images by combining protein expression and spatial information using CELESTA.

Nat Methods 2022 06 2;19(6):759-769. Epub 2022 Jun 2.

Department of Biomedical Data Science, School of Medicine, Stanford University, Stanford, CA, USA.

Advances in multiplexed in situ imaging are revealing important insights in spatial biology. However, cell type identification remains a major challenge in imaging analysis, with most existing methods involving substantial manual assessment and subjective decisions for thousands of cells. We developed an unsupervised machine learning algorithm, CELESTA, which identifies the cell type of each cell, individually, using the cell's marker expression profile and, when needed, its spatial information. We demonstrate the performance of CELESTA on multiplexed immunofluorescence images of colorectal cancer and head and neck squamous cell carcinoma (HNSCC). Using the cell types identified by CELESTA, we identify tissue architecture associated with lymph node metastasis in HNSCC, and validate our findings in an independent cohort. By coupling our spatial analysis with single-cell RNA-sequencing data on proximal sections of the same specimens, we identify cell-cell crosstalk associated with lymph node metastasis, demonstrating the power of CELESTA to facilitate identification of clinically relevant interactions.
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http://dx.doi.org/10.1038/s41592-022-01498-zDOI Listing
June 2022

Transcriptome and protein networks to elucidate the mechanism underlying nitrite degradation by Lactiplantibacillus plantarum.

Food Res Int 2022 Jun 10;156:111319. Epub 2022 May 10.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address:

Excessive nitrite residue is one of the bottlenecks in the production of many fermented foods. Lactiplantibacillus plantarum PK25 obtained from traditional Chinese pickles exhibited excellent nitrite degradation ability. Here, transcriptome, protein-protein interaction networks, and phenotype were performed to evaluate systematically the mechanism of nitrite degradation of L. plantarum PK25. The results demonstrated that genes expression varied considerably at key time points for nitrite degradation. 553 (upregulated: 366, downregulated: 187) and 767 (upregulated: 425, downregulated: 342) differentially expressed genes were identified at 6 h and 24 h, respectively. The hub genes were mainly enriched in carbohydrate metabolism, energy metabolism, and nucleotide synthesis. PK25 expanded its carbon source utilizing profile and improved glycolysis to produce more ATP to counteract environmental stress. The related enzymes including glycoside hydrolase, sugar ABC transporter protein, and PTS sugar transporter were 5.714, 5.885, and 3.578-fold upregulated at the transcriptional level. For strain to sustain energy levels and acid generation, pyruvate metabolism was critical, with the result that phosphoenolpyruvate synthase and pyruvate oxidase were up-regulated to accelerate the pyruvate transition. To repair DNA lesions induced by nitrite, both base excision repair mechanism and recombinational DNA repair pathway were exploited, such as endodeoxyribonuclease upregulated 5.314 and 19.687-fold at the two moments. The results provided a theoretical reference and practical possibility to reduce nitrite residue and improve safety during food fermented products.
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http://dx.doi.org/10.1016/j.foodres.2022.111319DOI Listing
June 2022

Oxidase-mimicking peptide-copper complexes and their applications in sandwich affinity biosensors.

Anal Chim Acta 2022 Jun 21;1214:339965. Epub 2022 May 21.

Henan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang Normal University, Anyang, Henan, 455000, PR China. Electronic address:

This work reported that the peptide-Cu complexes reveal oxidase-mimicking activities for oxygen reduction and ascorbate oxidation. The peptide features a histidine (His) residue in the first position with respect to the free N-terminal amine. The complexes can be easily integrated with nanomaterials such as gold nanoparticles (AuNPs) to form nanocatalysts or nanozymes, which allows for the fabrication of sensitive sensing platforms. To explore the analytical performances of the peptide-Cu complexes in biosensing, sandwich electrochemical and fluorescent immunoassays of prostate specific antigen (PSA) were performed with AuNP/peptide-Cu conjugates as the nanolabels. In the electrochemical immunoassays, the nanolabels coined as nanocatalysts promoted the electrocatalytic reduction of oxygen. For the fluorescent assays of PSA, the nanolabels coined as nanozymes catalyzed the oxidation of ascorbic acid (AA), and the resulting oxidization product dehydroascorbic acid (DHA) was reacted with o-phenylenediamine (OPD) to form fluorescent 3-(dihydroxyethyl)furo [3,4-b]quinoxaline-1-one (DFQ). The electrochemical and fluorescent signals are proportional to the concentration of PSA in the linear range of 0.001-0.50 and 0.001-2.00 ng/mL, respectively. The low detection limits of the methods (0.40 and 1.00 pg/mL) enabled clinical detection of PSA in serum samples. The findings should be valuable for understanding the biological functions of peptide-Cu complexes, and provide new insight to develop optical and electrochemical sensing platforms for clinical diagnosis.
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http://dx.doi.org/10.1016/j.aca.2022.339965DOI Listing
June 2022

Designing Robust Two-Electron Storage Extended Bipyridinium Anolytes for pH-Neutral Aqueous Organic Redox Flow Batteries.

JACS Au 2022 May 2;2(5):1214-1222. Epub 2022 May 2.

Department of Applied Chemistry, School of Chemistry and Material Science, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230026, China.

Bipyridinium derivatives represent the most extensively explored anolyte materials for pH-neutral aqueous organic redox flow batteries, and most derivatives feature two separate electron-transfer steps that cause a sharp decrease in cell voltage during discharge. Here, we propose a strategy to fulfill the concurrent two-electron electrochemical reaction by designing extended bipyridinium derivatives (exBPs) with a reduced energy difference between the lowest unoccupied molecular orbital of exBPs and the β-highest occupied molecular orbital of the singly reduced form. To demonstrate, a series of exBPs are synthesized and exhibit a single peak at redox potentials of -0.75 to -0.91 V (vs standard hydrogen electrode (SHE)), as opposed to the two peaks of most bipyridinium derivatives. Cyclic voltammetry along with diffusion-ordered spectroscopy and rotating disk electrode experiments confirm that this peak corresponds to a concurrent two-electron transfer. When examined in full-flowing cells, all exBPs demonstrate one charge/discharge plateau and two-electron storage. Continuous galvanostatic cell cycling reveals the side reactions leading to capacity fading, and we disclose the underlying mechanism by identifying the degradation products. By prohibiting the dimerization/β-elimination side reactions, we acquire a 0.5 M (1 M e) exDMeBP/FcNCl cell with a high capacity of 22.35 Ah L and a capacity retention rate of 99.95% per cycle.
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http://dx.doi.org/10.1021/jacsau.2c00184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131478PMC
May 2022

Urinary paraben derivatives in pregnant women at three trimesters: Variability, predictors, and association with oxidative stress biomarkers.

Environ Int 2022 Jul 16;165:107300. Epub 2022 May 16.

Key Laboratory of Environment and Health (HUST), Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China. Electronic address:

Exposure to parabens has been shown to increase oxidative stress, which has a vital impact on the development of numerous diseases. However, few studies reported the effects of the paraben derivatives on oxidative stress, particularly among pregnant women. This study, using repeated measurements, aimed to understand the exposure profiles of urinary paraben derivative concentrations and their relationships with oxidative stress biomarkers (OSBs). A total of 861 pregnant women, who provided spot urine samples at three trimesters, were included, and 2583 urine samples were used to measure four paraben derivatives [p-hydroxybenzoic acid (p-HB), 3,4-dihydroxybenzoic acid (3,4-DHB), methyl protocatechuate, and ethyl protocatechuate], four parabens (methyl, ethyl, propyl, and butyl), and three OSBs [8-hydroxy-2'-deoxyguanosine (for DNA), 8-hydroxyguanosine (for RNA), and 4-hydroxy nonenal mercapturic acid (for lipid)]. Pregnant women were extensively exposed to parabens and paraben derivatives with detection frequencies (DFs) of 86.1%-100%, except for butylparaben with a DF of 14.9%. p-HB and 3,4-DHB had relatively high urinary concentrations (specific gravity-adjusted median values: 1394 and 74.5 ng/mL, respectively). Low reproducibility in paraben derivatives was found across the three trimesters. Sampling season, pre-pregnancy body mass index, and infant sex were predictors of some paraben derivatives/parabens. Linear mixed model analyses showed that all target compounds (if DF > 50%) were associated with increases in all the selected OSBs, where the percent change in OSBs with an interquartile range increase in paraben concentration ranged from 9.85% to 24.7%, while those in paraben derivative concentration ranged from 13.8% to 72.1%. Weighted quantile sum model showed that joint exposure was significantly associated with increased OSBs, and paraben derivatives were stronger contributors to OSBs compared with parabens. Overall, urinary paraben derivatives were associated with increased oxidative stress of nucleic acids and lipid in pregnant women.
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http://dx.doi.org/10.1016/j.envint.2022.107300DOI Listing
July 2022
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