Publications by authors named "Yuanyuan Huang"

213 Publications

Analysis of the comprehensive non-pharmaceutical interventions and measures in containing the COVID-19 epidemic in Shenzhen: a retrospective study.

BMJ Open 2021 Jul 26;11(7):e044940. Epub 2021 Jul 26.

Department of Communicable Disease Control and Prevention, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, China

Objective: To analyse the epidemiological characteristics of family clusters of COVID-19 and the three stages of the comprehensive non-pharmaceutical interventions and measures implemented in Shenzhen.

Methods: The epidemic curve of COVID-19 was drawn and the impact of the comprehensive non-pharmaceutical interventions and measures was analysed by the different periods of the epidemic.

Results: A total of 427 cases (417 confirmed cases and 10 asymptomatic infectious cases) were reported in Shenzhen, of which 259 (60.7%) were clustered cases. 97 cluster events were drawn and most cluster events (97.3%) occurred in families. There were three stages of the COVID-19 epidemic in Shenzhen. The epidemic increased rapidly, but the peak lasted for a short time, while the decline in incidence was rapid and large.

Conclusions: Family clusters were the main feature of the COVID-19 outbreak in Shenzhen in 2020, and the Shenzhen government rolled out a quick response to the epidemic. Non-pharmaceutical interventions and measures were proven to have effectively contained community transmission, limit the transmission to aggregation and reduce the scale of transmission within a household.
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http://dx.doi.org/10.1136/bmjopen-2020-044940DOI Listing
July 2021

Pediatric Asthma Situation in Chengdu, China, During the COVID-19 Pandemic: An Observational Study.

J Asthma Allergy 2021 9;14:829-838. Epub 2021 Jul 9.

Department of Pediatric Pulmonology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Background: To tackle the COVID-19 pandemic and mitigate viral transmission, mainland China has implemented various disease prevention measures and arrangements. We hypothesize that these measures may be pose challenges to the management of pediatric asthma. Here, we studied the situation of pediatric asthma in Chengdu during the COVID-19 pandemic and compared the pediatric asthma situation before so as to provide a reference for designing effective management plan for pediatric asthma patients in the future facing the outbreak of epidemic.

Methods: An observational study was done to compare the pediatric asthma situation in Chengdu from 2017 to 2019 to the situation under COVID-19 pandemic. Asthma incidence, severe asthma attack, air quality, temperature, and patient follow-up were examined.

Results: The number of monthly asthma cases decreased significantly in February 2020. The number of asthma cases in 2017-2019 positively correlated with levels of particulate matter PM (p = 0.006) and PM (p = 0.005), while it negatively correlated with temperature (p = 0.048). No correlation was identified in 2020. A higher rate of severe asthma attack cases (9.69%) was observed among asthma patients in 2020 (p = 0.014). Differences were identified between the monthly severe asthma attack during the period 2017-2020 (p<0.001). The rate of severe asthma attack cases peaked in June and September 2020. The percentage of patients who failed to undergo pulmonary function tests was 34.5% in 2020, remarkably higher than in the previous 3 years (p<0.001).

Conclusion: The situation and management of pediatric asthma during the 2020 COVID-19 pandemic differed from those in previous years, with more emphasis placed on disease prevention practices and facilities. To design future pediatric asthma management practice, the environmental and psychological impact on asthma management should also be considered. Local areas should make good use of telemedicine to manage pediatric asthma effectively.
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http://dx.doi.org/10.2147/JAA.S315695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277453PMC
July 2021

Resveratrol-induced Sirt1 phosphorylation by LKB1 mediates mitochondrial metabolism.

J Biol Chem 2021 Jul 1;297(2):100929. Epub 2021 Jul 1.

MOE Key Laboratory for Cellular Dynamics, University of Science & Technology of China School of Life Sciences, Hefei, China; Anhui Key Laboratory for Cellular Dynamics & Chemical Biology, CAS Center for Excellence in Molecular Cell Science & Hefei National Science Center for Physical Sciences at Microscale, Hefei, Anhui, China; Keck Center for Organoids Plasticity, Morehouse School of Medicine, Atlanta, Georgia, USA. Electronic address:

The NAD-dependent deacetylase Sirt1 has been implicated in the prevention of many age-related diseases, including cancer, type 2 diabetes, and cardiovascular disease. Resveratrol, a plant polyphenol, exhibits antiaging, antitumor, and vascular protection effects by activating Sirt1. However, the molecular mechanism of Sirt1 activation as induced by resveratrol remains unclear. By knockdown/rescue experiments, fluorometric Sirt1 activity assay, immunoprecipitation, and pull-down assays, we identify here that the tumor suppressor LKB1 (liver kinase B1) as a direct activator of Sirt1 elicited by resveratrol. Resveratrol promotes the binding between LKB1 and Sirt1, which we first reported, and this binding leads to LKB1-mediated phosphorylation of Sirt1 at three different serine residues in the C terminus of Sirt1. Mechanistically, LKB1-mediated phosphorylation increases intramolecular interactions in Sirt1, such as the binding of the C terminus to the deacetylase core domain, thereby eliminating DBC1 (Deleted in Breast Cancer 1, Sirt1 endogenous inhibitor) inhibition and promoting Sirt1-substrate interaction. Functionally, LKB1-dependent Sirt1 activation increases mitochondrial biogenesis and respiration through deacetylation and activation of the transcriptional coactivator PGC-1α. These results identify Sirt1 as a context-dependent target of LKB1 and suggest that a resveratrol-stimulated LKB1-Sirt1 pathway plays a vital role in mitochondrial metabolism, a key physiological process that contributes to numerous age-related diseases.
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http://dx.doi.org/10.1016/j.jbc.2021.100929DOI Listing
July 2021

A Simple Culture Method Enhances the Recovery of Culturable Actinobacteria From Coastal Sediments.

Front Microbiol 2021 14;12:675048. Epub 2021 Jun 14.

College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China.

Molecular methods revealed that the majority of microbes in natural environments remains uncultivated. To fully understand the physiological and metabolic characteristics of microbes, however, culturing is still critical for microbial studies. Here, we used bacterial community analysis and four culture media, namely, traditional marine broth 2216 (MB), water extracted matter (WEM), methanol extracted matter (MEM), and starch casein agar (SCA), to investigate the diversity of cultivated bacteria in coastal sediments. A total of 1,036 isolates were obtained in pure culture, and they were classified into five groups, namely, Alphaproteobacteria (52.51%), Gammaproteobacteria (23.26%), Actinobacteria (13.32%), Firmicutes, and Bacteroidetes. Compared to other three media, WEM recovered a high diversity of actinobacteria (42 of 63 genotypes), with and as the most cultivated genera. Amplicon sequencing of the bacterial 16S ribosomal RNA (rRNA) gene V3-V4 fragment revealed eight dominant groups, Alphaproteobacteria (12.81%), Gammaproteobacteria (20.07%), Deltaproteobacteria (12.95%), Chloroflexi (13.09%), Bacteroidetes (8.28%), Actinobacteria (7.34%), Cyanobacteria (6.20%), and Acidobacteria (5.71%). The dominant members affiliated to Actinobacteria belonged to " Actinomarinales," " Microtrichales," and Nitriliruptorales. The cultivated actinobacteria accounted for a small proportion (<5%) compared to the actinobacterial community, which supported that the majority of actinobacteria are still waiting for cultivation. Our study concluded that WEM could be a useful and simple culture medium that enhanced the recovery of culturable actinobacteria from coastal sediments.
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http://dx.doi.org/10.3389/fmicb.2021.675048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236954PMC
June 2021

Differences in the Association of Anxiety, Insomnia and Somatic Symptoms between Medical Staff and the General Population During the Outbreak of COVID-19.

Neuropsychiatr Dis Treat 2021 14;17:1907-1915. Epub 2021 Jun 14.

Department of Psychiatry, The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, People's Republic of China.

Background: During the outbreak of coronavirus disease 2019 (COVID-19), occupational differences were recognized with respect to psychological issues, but there are no reports regarding the insomnia and anxiety comorbidities and their related factors among the general public and medical staff. Our study aimed to compare the prevalence of anxiety and anxiety with insomnia, as well as the relationship between different psychological symptoms between the two groups.

Methods: A total of 605 medical staff and 1091 public respondents were assessed through an online questionnaire survey, including the 7-item Insomnia Severity Index (ISI), the somatization subscale of Symptom Checklist 90-Revised (SCL-90-R), and the 7-item Generalized Anxiety Disorder scale (GAD-7).

Results: Compared with the general public, medical staff had a higher incidence of anxiety (45.5% vs 32.4%). The incidence of insomnia in anxious participants was 52.7% in medical staff and 44.6% in the general public, and the difference was significant (p < 0.05). The GAD-7 score and somatization subscore of the SCI-90 were independently associated with insomnia among anxiety participants in both groups (all p < 0.001).

Conclusion: Our findings suggest that during the epidemic period of COVID-19, medical staff experienced more anxiety and anxiety with insomnia. Somatization, anxiety and insomnia are highly correlated among medical staff and the general public.
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http://dx.doi.org/10.2147/NDT.S300719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214107PMC
June 2021

Differences in the Association of Anxiety, Insomnia and Somatic Symptoms between Medical Staff and the General Population During the Outbreak of COVID-19.

Neuropsychiatr Dis Treat 2021 14;17:1907-1915. Epub 2021 Jun 14.

Department of Psychiatry, The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, People's Republic of China.

Background: During the outbreak of coronavirus disease 2019 (COVID-19), occupational differences were recognized with respect to psychological issues, but there are no reports regarding the insomnia and anxiety comorbidities and their related factors among the general public and medical staff. Our study aimed to compare the prevalence of anxiety and anxiety with insomnia, as well as the relationship between different psychological symptoms between the two groups.

Methods: A total of 605 medical staff and 1091 public respondents were assessed through an online questionnaire survey, including the 7-item Insomnia Severity Index (ISI), the somatization subscale of Symptom Checklist 90-Revised (SCL-90-R), and the 7-item Generalized Anxiety Disorder scale (GAD-7).

Results: Compared with the general public, medical staff had a higher incidence of anxiety (45.5% vs 32.4%). The incidence of insomnia in anxious participants was 52.7% in medical staff and 44.6% in the general public, and the difference was significant (p < 0.05). The GAD-7 score and somatization subscore of the SCI-90 were independently associated with insomnia among anxiety participants in both groups (all p < 0.001).

Conclusion: Our findings suggest that during the epidemic period of COVID-19, medical staff experienced more anxiety and anxiety with insomnia. Somatization, anxiety and insomnia are highly correlated among medical staff and the general public.
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http://dx.doi.org/10.2147/NDT.S300719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214107PMC
June 2021

A comprehensive assessment of genetic variation in serotonin transporter gene (5-HTTLPR+rs25531) and the response to dapoxetine in Chinese patients with premature ejaculation.

Andrologia 2021 Jun 12:e14141. Epub 2021 Jun 12.

Department of Urology, First Affiliated Hospital of Anhui Medical University, Hefei, China.

This study was to explore whether serotonin transporter gene-linked polymorphic region polymorphisms (5-HTTLPR+rs25531) influence the response to dapoxetine treatment in a Chinese population with premature ejaculation (PE). 112 patients with PE re-enrolled from our previous study received dapoxetine monotherapy. At the endpoint, patients with S'S' had a significant increased risk of nonresponse compared with L' carriers (p < .001). The improvement in S'S' genotype was significantly lower in premature ejaculation profile (PEP) items of 'control over ejaculation' (p = .035) and 'distress related to ejaculation' (p = .017) than that in L' carriers. As to clinical global impression of change (CGIC), results in S'S' subjects showed significantly lower scores (p = .008) and a less satisfaction rate reporting at least 'better' (p = .020) compared with L' carriers. Moreover, our findings suggested that patients with S'S' were more likely to develop adverse effects (AEs) compared with L' carriers (p = .040). This study suggests that PE patients bearing the S'S' genotype have an inferior comprehensive efficacy and safety of dapoxetine treatment, which consist of poorer response in IELTs, less improvement in patient-reported outcome (PRO) measures and greater incidence of AEs, than L' carriers. Variants of triallelic 5-HTTLPR may play a major role as a predictor of treatment response to dapoxetine.
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http://dx.doi.org/10.1111/and.14141DOI Listing
June 2021

Expression of brain-derived neurotrophic factor in rapid ejaculator rats: A further study.

Andrologia 2021 Jun 8:e14134. Epub 2021 Jun 8.

Department of Urology, First Affiliated Hospital of Anhui Medical University, Hefei, China.

Limited evidence has indicated that brain-derived neurotrophic factor (BDNF) may be involved in the neurobiology of premature ejaculation (PE). This study aimed to investigate BDNF levels in the central and peripheral nervous systems of a rapid ejaculation model. Eighteen male rats were selected and classified as 'sluggish', 'normal' and 'rapid' ejaculators on the basis of ejaculation frequency during copulatory behavioural tests. BDNF levels in specific brain regions, spinal cord and serum were determined by enzyme-linked immunosorbent assay (ELISA). Consistent with the results in PE patients, the concentration of serum BDNF decreased significantly from the sluggish rats to normal and rapid rats. Besides, in both brain regions and spinal cord, the sluggish group had the highest BDNF levels, while the rapid group had the lowest BDNF levels. Regression analyses of the expression of BDNF presented positive correlations between serum and brain (r = 0.958, p < .001), and between serum and spinal cord (r = 0.967, p < .001) respectively. Our findings suggested insufficient BDNF in the nervous system and serum may lead to rapid ejaculation. The current study adds to the evidence that BDNF is involved in the regulation of ejaculation.
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http://dx.doi.org/10.1111/and.14134DOI Listing
June 2021

Dispersion Property and Evolution of Second Harmonic Generation Pattern in Type-I and Type-II van der Waals Heterostructures.

ACS Appl Mater Interfaces 2021 Jun 7;13(23):27334-27342. Epub 2021 Jun 7.

Shaanxi Joint Lab of Graphene, International Collaborative Center on Photoelectric Technology and Nano Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics & Photon-Technology, Northwest University, Xi'an 710069, China.

Dispersion property and second harmonic generation (SHG) pattern of novel two-dimensional (2D) van der Waals heterostructures (vdWHs) is of great significance not only for the characterization of material symmetry but also for understanding nonlinear photophysical phenomena. Herein, we demonstrate the SHG response of 2D type-I (MoTe/WSe) and type-II (MoSe/WSe) band alignment of vdWHs. In the dispersion relation of the second-order nonlinear coefficient, the pronounced peaks of the element for both vdWHs are mainly contributed by resonance in the interband transition processes, whereas other elements are derived from the intraband transition processes because of the highly efficient charge transfer from WSe to MoTe in type-I vdWHs and the ultrafast charge separation between WSe and MoSe in type-II vdWHs, respectively. Besides, more nonzero nonlinear coefficient elements can participate in a nonlinear response at the oblique incidence, to which special attention needs paid. The polarization angle α-dependent SHG patterns display a rotational fourfold symmetry, whereas the azimuthal angle ϕ-dependent SHG patterns show sixfold symmetry for both type-I and type-II vdWHs at any wavelength under normal incidence. Under oblique incidence, the α-dependent (ϕ-dependent) SHG patterns will reduce to twofold (threefold) symmetry for both vdWHs. The results highlight the potential to deterministically engineer novel nonlinear optical properties for tunable anisotropic applications of nonlinear optoelectronic devices based on vdWHs.
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http://dx.doi.org/10.1021/acsami.1c07441DOI Listing
June 2021

Glycolysis-Based Genes Are Potential Biomarkers in Thyroid Cancer.

Front Oncol 2021 26;11:534838. Epub 2021 Apr 26.

Clinical Research Center, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.

While increased glycolysis has been identified as a cancer marker and attracted much attention in thyroid cancer (THCA), the prognostic role of it remains to be further elucidated. Here we aimed to determine a specific glycolysis-associated risk model to predict THCA patients' survival. We also explored the interaction between this signature and tumor immune microenvironment and performed drug screening to identify specific drugs targeting the glycolysis-associated signature. Six genes (CHST6, POM121C, PPFIA4, STC1, TGFBI, and FBP2) comprised the specific model, which was an independent prognostic indicator in THCA patients determined by univariate, LASSO and multivariate Cox regression analyses. The receiver operating characteristic (ROC) curve analysis confirmed the excellent clinical performance of the prognostic signature. According to the specific gene signature, patients were categorized into high- and low-risk subgroups. The high-risk group was characterized by decreased immune score and elevated tumor purity, as well as worser survival prognosis compared to the low-risk group. We also validated the expression of these genes in clinical samples and experiments. Lastly, we identified potential drugs targeting the glycolysis-associated signature. The derived glycolysis-related signature is an independent prognostic biomarker for THCA patients and might be used as an efficacy of biomarker for drug-sensitivity prediction.
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http://dx.doi.org/10.3389/fonc.2021.534838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107473PMC
April 2021

The gut microbiome is associated with brain structure and function in schizophrenia.

Sci Rep 2021 May 7;11(1):9743. Epub 2021 May 7.

Department of Biomedical Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou, 510006, China.

The effect of the gut microbiome on the central nervous system and its possible role in mental disorders have received increasing attention. However, knowledge about the relationship between the gut microbiome and brain structure and function is still very limited. Here, we used 16S rRNA sequencing with structural magnetic resonance imaging (sMRI) and resting-state functional (rs-fMRI) to investigate differences in fecal microbiota between 38 patients with schizophrenia (SZ) and 38 demographically matched normal controls (NCs) and explored whether such differences were associated with brain structure and function. At the genus level, we found that the relative abundance of Ruminococcus and Roseburia was significantly lower, whereas the abundance of Veillonella was significantly higher in SZ patients than in NCs. Additionally, the analysis of MRI data revealed that several brain regions showed significantly lower gray matter volume (GMV) and regional homogeneity (ReHo) but significantly higher amplitude of low-frequency fluctuation in SZ patients than in NCs. Moreover, the alpha diversity of the gut microbiota showed a strong linear relationship with the values of both GMV and ReHo. In SZ patients, the ReHo indexes in the right STC (r = - 0.35, p = 0.031, FDR corrected p = 0.039), the left cuneus (r = - 0.33, p = 0.044, FDR corrected p = 0.053) and the right MTC (r = - 0.34, p = 0.03, FDR corrected p = 0.052) were negatively correlated with the abundance of the genus Roseburia. Our results suggest that the potential role of the gut microbiome in SZ is related to alterations in brain structure and function. This study provides insights into the underlying neuropathology of SZ.
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http://dx.doi.org/10.1038/s41598-021-89166-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105323PMC
May 2021

Altered Heart Rate Variability in Patients With Schizophrenia During an Autonomic Nervous Test.

Front Psychiatry 2021 30;12:626991. Epub 2021 Mar 30.

Department of Biomedical Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou, China.

Reduced heart rate variability (HRV) and dysfunction of the autonomic nervous system (ANS) have been observed in schizophrenia patients. HRV parameters of schizophrenia patients in the resting state have been well-documented; however, these parameters of schizophrenia patients who experience continuous psychophysiological stress remain unclear. The objective of this study was to systematically explore the linear and nonlinear HRV parameters between schizophrenia patients and normal controls and to detect the adaptive capabilities of HRV of schizophrenia patients during the stimulation tests of autonomic nervous system. Forty-five schizophrenia patients and forty-five normal controls, matched for age, sex and body mass index, completed a 14 min ANS test. Thirteen linear and nonlinear HRV parameters of all subjects under the ANS test were computed and statistically analyzed between groups and between sessions. The STROBE checklist was adhered to in this study. All time-domain HRV features in the ANS test were significantly different between schizophrenia patients and normal controls ( < 0.01). The schizophrenia patients showed significantly low values in the Poincaré indices, which revealed significantly decreased heart rate fluctuation complexity compared with that of normal controls ( < 0.001). In addition, the normal controls, not schizophrenia patients, showed significant differences between the recovery and stress states in the parameters of low frequency, high frequency, and nonlinear dynamics. Schizophrenia patients showed autonomic dysfunction of the heart in a series of stimulation tests of the autonomic nervous system and could not regain normal physiological functions after stress cessation. Our findings revealed that the dynamic parameters of HRV in psychophysiological stress are sensitive and practical for a diagnosis of schizophrenia.
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http://dx.doi.org/10.3389/fpsyt.2021.626991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074969PMC
March 2021

AMPKα2 activation by an energy-independent signal ensures chromosomal stability during mitosis.

iScience 2021 Apr 26;24(4):102363. Epub 2021 Mar 26.

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China School of Life Sciences, Hefei 230027, China.

AMP-activated protein kinase (AMPK) senses energy status and impacts energy-consuming events by initiating metabolism regulatory signals in cells. Accumulating evidences suggest a role of AMPK in mitosis regulation, but the mechanism of mitotic AMPK activation and function remains elusive. Here we report that AMPKα2, but not AMPKα1, is sequentially phosphorylated and activated by CDK1 and PLK1, which enables AMPKα2 to accurately guide chromosome segregation in mitosis. Phosphorylation at Thr485 by activated CDK1-Cyclin B1 brings the ST-stretch of AMPKα2 to the Polo box domain of PLK1 for subsequent Thr172 phosphorylation by PLK1. Inserting of the AMPKα2 ST-stretch into AMPKα1, which lacks the ST-stretch, can correct mitotic chromosome segregation defects in AMPKα2-depleted cells. These findings uncovered a specific signaling cascade integrating sequential phosphorylation by CDK1 and PLK1 of AMPKα2 with mitosis to maintain genomic stability, thus defining an isoform-specific AMPKα2 function, which will facilitate future research on energy sensing in mitosis.
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http://dx.doi.org/10.1016/j.isci.2021.102363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059055PMC
April 2021

Signal Identification of Gear Vibration in Engine-Gearbox Systems Based on Auto-Regression and Optimized Resonance-Based Signal Sparse Decomposition.

Sensors (Basel) 2021 Mar 7;21(5). Epub 2021 Mar 7.

The State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, No. 38, Zheda Road, Hangzhou 310027, China.

As an essential part of the transmission system, gearboxes are considered as a major source of vibration. Signal identification of gear vibration is necessary for online monitoring of the mechanical systems. However, in engine-gearbox systems, the ignition impact of the engine is strong, so that the gear vibration is generally submerged. To overcome this issue, the resonance-based signal sparse decomposition (RSSD) method is used in this paper based on different oscillatory behaviors of the gear meshing impact and the engine ignition impact. To improve the accuracy of RSSD under interferences, the meshing frequency energy ratio (MF-ER) index is introduced into RSSD to adaptively choose the decomposition parameters. Before applying the RSSD method, the auto-regression (AR) model is used as a pre-whitening step to eliminate the normal gear meshing vibration, which improves the decomposition performance of RSSD. The effectiveness of the proposed AR-ORSSD (AR-based optimized RSSD) algorithm is tested using both simulated signals and measured vibration signals from an engine-gearbox system in a forklift. Comparisons were made with the RSSD algorithm based on a genetic algorithm. Experimental results indicate that the AR-ORSSD algorithm is superior at identifying gear vibration signals especially when under strong interferences.
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http://dx.doi.org/10.3390/s21051868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962202PMC
March 2021

Systematic Design of a Frustrated Lewis Pair Containing Methyleneborane and Carbene for Dinitrogen Activation.

Inorg Chem 2021 Apr 31;60(8):5598-5606. Epub 2021 Mar 31.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Fujian Provincial Key Laboratory of Theoretical and Computational Chemistry, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China.

Activation of atmospherically abundant dinitrogen (N) by metal-free species under mild reaction conditions has been one of the most challenging areas in chemistry for decades. Very recent but limited progress in N activation by boron species, including two-coordinated borylene and methyleneborane and three-coordinated borole and borane, has been made toward metal-free N activation. Here, we systematically probe an experimentally viable frustrated Lewis pair (FLP) containing two moieties (methyleneborane and carbene) for N activation via density functional theory (DFT) calculations, which has proven to be an efficient approach for N activation in a thermodynamically and kinetically favorable manner. Aromaticity is found to play a crucial role in stabilization of the product. This study could be a valuable alternative for the development of metal-free N activation chemistry, highlighting great potential of FLP for N activation and functionalization.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03520DOI Listing
April 2021

The Effects of Repetitive Transcranial Magnetic Stimulation in Patients with Chronic Schizophrenia: Insights from EEG Microstates.

Psychiatry Res 2021 May 9;299:113866. Epub 2021 Mar 9.

Department of Biomedical Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou 510006, China; The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou Huiai Hospital, Guangzhou 510370, China; Guangdong Engineering Technology Research Center for Translational Medicine of Mental Disorders, Guangzhou 510370, China; Guangdong Engineering Technology Research Center for Diagnosis and Rehabilitation of Dementia, Guangzhou 510500, China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, China; National Engineering Research Center for Healthcare Devices, Guangzhou 510500, China; Department of Nuclear Medicine and Radiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan 980-8575. Electronic address:

The objective of this study was to investigate the effects of 10 Hz repetitive transcranial magnetic stimulation (rTMS) in patients with schizophrenia using EEG microstates. Thirty-eight patients with chronic schizophrenia were included in a double-blind, randomized and sham-controlled trial (19 participants in the active group and 19 participants in the sham group) and received 10 Hz active or sham rTMS stimulation to the left dorsolateral prefrontal cortex (left DLPFC) 5 days per week over for 4 weeks. Four classical microstate classes (i.e., classes A, B, C and D) were identified by clustering, and the parameters (i.e., duration, occurrence and contribution) of each class were computed. Our results showed that (1) after stimulation, the positive and negative syndrome scale (PANSS) positive scores decreased significantly in the active group; (2) the duration of the microstate of class C derived from EEG data decreased significantly in the active group; and (3) the change of the duration of class D in the active group was significantly higher than that in the sham group. Our findings demonstrated that 10 Hz active rTMS stimulation was beneficial to improving the positive symptoms of patients with chronic schizophrenia, and the EEG microstate could be an effective indicator of symptom improvements.
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http://dx.doi.org/10.1016/j.psychres.2021.113866DOI Listing
May 2021

Poor Status of Vitamin D: A Survey of Area With Lowest Sunlight Radiation in Sichuan, China.

Front Endocrinol (Lausanne) 2021 24;12:626983. Epub 2021 Feb 24.

Department of Endocrinology, West China Hospital of Sichuan University, Chengdu, China.

Objective: Vitamin D plays an important role in bone and mineral metabolism. Ultraviolet B (UVB) is the primary determinant for vitamin D synthesis. However, population-based data of vitamin D status was sparse in areas with sunlight deprivation in China. This study aimed to assess serum 25-hydroxyvitamin D [25(OH)D] levels among adult women in Sichuan basin with the lowest sunlight radiation in China, and the associations with sunlight exposure and age.

Methods: In the context of the same ethnicity, similar latitude and lifestyle in sunlight-limited basin and sunlight-abundant plateau, 1,057 women in basin and 337 in plateau aged 29-95 years were included in this study, from November 2012 to February 2013. Daily sunlight exposure duration of previous month was obtained using questionnaires. Serum 25(OH)D was measured by enzyme-linked immunosorbent assay.

Results: The prevalence of vitamin D severe deficiency [25(OH)D <30 nmol/L] and deficiency [30 ≤ 25(OH)D <50 nmol/L] was significantly higher in basin than plateau (21.85% vs. 10.09%, and 59.32% vs. 40.36%; <0.0001). Women from basin exhibited lower serum 25(OH)D levels than those from plateau (40.66 ± 15.62 vs. 52.54 ± 19.94 nmol/L, <0.0001). In basin, women more than 50 years old had higher 25(OH)D than younger counterparts, and 25(OH)D level of these groups was not associated with sunlight exposure duration. While in plateau, women younger than 60 years old had higher 25(OH)D than the older women. Furthermore, for those younger groups, women with long sunlight exposure (≥3 h daily) had higher 25(OH)D concentration than those with short sunlight exposure (<3 h daily). Serum PTH was negatively associated with 25(OH)D in basin, but not in plateau.

Conclusions: Alarmingly high prevalence of vitamin D deficiency was observed in women in sunlight-deprived basin in Sichuan. Only the vitamin D status of younger women from plateau with adequate solar radiation could benefit from sunlight exposure. Vitamin D supplementation and vitamin D-fortified food should be encouraged to improve vitamin D status for women living in sunlight-limited areas, or with old age.
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http://dx.doi.org/10.3389/fendo.2021.626983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959743PMC
February 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cell Mol Immunol 2021 04 11;18(4):969-978. Epub 2021 Mar 11.

CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

T follicular helper (Tfh) cells are crucial for regulating autoimmune inflammation and protective immunity against viral infection. However, the molecular mechanism controlling Tfh cell differentiation is poorly understood. Here, through two mixed bone marrow chimeric experiments, we identified Peli1, a T cell-enriched E3 ubiquitin ligase, as an intrinsic regulator that inhibits Tfh cell differentiation. Peli1 deficiency significantly promoted c-Rel-mediated inducible T-cell costimulator (ICOS) expression, and PELI1 mRNA expression was negatively associated with ICOS expression on human CD4 T cells. Mechanistically, increased ICOS expression on Peli1-KO CD4 T cells enhanced the activation of PI3K-AKT signaling and thus suppressed the expression of Klf2, a transcription factor that inhibits Tfh differentiation. Therefore, reconstitution of Klf2 abolished the differences in Tfh differentiation and germinal center reaction between WT and Peli1-KO cells. As a consequence, Peli1-deficient CD4 T cells promoted lupus-like autoimmunity but protected against H1N1 influenza virus infection in mouse models. Collectively, our findings established Peli1 as a critical negative regulator of Tfh differentiation and indicated that targeting Peli1 may have beneficial therapeutic effects in Tfh-related autoimmunity or infectious diseases.
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http://dx.doi.org/10.1038/s41423-021-00660-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115645PMC
April 2021

Deep Learning Optimizes Data-Driven Representation of Soil Organic Carbon in Earth System Model Over the Conterminous United States.

Front Big Data 2020 3;3:17. Epub 2020 Jun 3.

Department of Biological Sciences, Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, AZ, United States.

Soil organic carbon (SOC) is a key component of the global carbon cycle, yet it is not well-represented in Earth system models to accurately predict global carbon dynamics in response to climate change. This novel study integrated deep learning, data assimilation, 25,444 vertical soil profiles, and the Community Land Model version 5 (CLM5) to optimize the model representation of SOC over the conterminous United States. We firstly constrained parameters in CLM5 using observations of vertical profiles of SOC in both a batch mode (using all individual soil layers in one batch) and at individual sites (site-by-site). The estimated parameter values from the site-by-site data assimilation were then either randomly sampled (random-sampling) to generate continentally homogeneous (constant) parameter values or maximally preserved for their spatially heterogeneous distributions (varying parameter values to match the spatial patterns from the site-by-site data assimilation) so as to optimize spatial representation of SOC in CLM5 through a deep learning technique (neural networking) over the conterminous United States. Comparing modeled spatial distributions of SOC by CLM5 to observations yielded increasing predictive accuracy from default CLM5 settings ( = 0.32) to randomly sampled (0.36), one-batch estimated (0.43), and deep learning optimized (0.62) parameter values. While CLM5 with parameter values derived from random-sampling and one-batch methods substantially corrected the overestimated SOC storage by that with default model parameters, there were still considerable geographical biases. CLM5 with the spatially heterogeneous parameter values optimized from the neural networking method had the least estimation error and less geographical biases across the conterminous United States. Our study indicated that deep learning in combination with data assimilation can significantly improve the representation of SOC by complex land biogeochemical models.
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http://dx.doi.org/10.3389/fdata.2020.00017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931903PMC
June 2020

Persons with pre-dementia have no Kantian duty to die.

Bioethics 2021 Jun 8;35(5):438-445. Epub 2021 Mar 8.

Peking University Health Science Centre, Beijing, China.

Cooley's argument that persons with pre-dementia have a Kantian duty to die has led to much debate. Cooley gives two reasons for his claim, the first being that a person with pre-dementia should end his/her life when he/she will inevitably and irreversibly lose rationality and be unable to live morally as a result. This paper argues that this reason derives from an unsubstantiated premise and general confusion regarding the condition for a Kantian duty to die. Rather, a close reading of Kant reveals that such a condition occurs when a person confronts an external handicap that does not undermine his/her rational ability but deprives him/her of the possibility of living the way a person should. People do not confront this experience with progressive dementia. The other reason Cooley proposes is that a person should not allow their continued existence to become a burden to others. This claim partly stems from a radical interpretation of a case discussed by Kant and is partly based on a misuse of Kant's formulation of humanity. Based on a prudent inference from Kantian ethics, this article argues against Cooley that persons with pre-dementia have no Kantian duty to die.
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http://dx.doi.org/10.1111/bioe.12865DOI Listing
June 2021

Noncoding RNAs involved in DNA methylation and histone methylation, and acetylation in diabetic vascular complications.

Pharmacol Res 2021 Feb 25:105520. Epub 2021 Feb 25.

Department of Pediatrics in First Hospital, Jilin University, Changchun 130021, PR China. Electronic address:

Diabetes is a metabolic disorder and its incidence is still increasing. Diabetic vascular complications cause major diabetic mobility and include accelerated atherosclerosis, nephropathy, retinopathy, and neuropathy. Hyperglycemia contributes to the pathogenesis of diabetic vascular complications via numerous mechanisms including the induction of oxidative stress, inflammation, metabolic alterations, and abnormal proliferation of EC and angiogenesis. In the past decade, epigenetic modifications have attracted more attention as they participate in the progression of diabetic vascular complications despite controlled glucose levels and regulate gene expression without altering the genomic sequence. DNA methylation and histone methylation, and acetylation are vital epigenetic modifications and their underlying mechanisms in diabetic vascular complication are still urgently needed to be investigated. Non-coding RNAs (nc RNAs) such as micro RNAs (miRNAs), long non-coding RNA (lncRNAs), and circular RNAs (circ RNAs) were found to exert transcriptional regulation in diabetic vascular complication. Although nc RNAs are not considered as epigenetic components, they are involved in epigenetic modifications. In this review, we summarized the investigations of non-coding RNAs involved in DNA methylation and histone methylation and acetylation. Their cross-talks might offer novel insights into the pathology of diabetic vascular complications.
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http://dx.doi.org/10.1016/j.phrs.2021.105520DOI Listing
February 2021

Stressors, coping styles, and anxiety & depression in pediatric nurses with different lengths of service in six tertiary hospitals in Chengdu, China.

Transl Pediatr 2020 Dec;9(6):827-834

Department of Pediatric Respiratory Medicine, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: The level of stress experienced by nurses is related to their length of service. In the current study, we investigated the potential correlations among stressors, coping styles, and anxiety and depression in pediatric nurses with different lengths of service in six tertiary hospitals in Chengdu, Sichuan, China.

Methods: Between January and June 2018, we enrolled 500 pediatric nurses from 6 tertiary hospitals in Chengdu using a convenience sampling method. A cross-sectional survey was carried out using the Chinese Nurses' Occupational Stressor Scale (NOSS), the Simplified Coping Style Questionnaire (SCSQ), and Zung's Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS).

Results: Statistically significant differences were found in the average scores of NOSS and scores of various dimensions, SAS and SDS, and Simple Coping Style score among pediatric nurses with different lengths of service (all P<0.05). Nurses with 8-12 years of service had the highest average score for stressors. Anxiety and depression were both prevalent among nurses with 4-7 years of service. The average overall stress scores of nurses with different lengths of service were negatively correlated with positive coping style (P<0.05), were not significantly correlated with negative coping style (P>0.05), and were positively correlated with anxiety score and depression score (P<0.05). The positive coping style score showed negative correlations with anxiety score and depression score (both P<0.05). The negative coping style score was positively correlated with the scores for anxiety and depression (all P<0.05), except in nurses with 4-7 years of service, for whom the negative coping style score showed no significant correlation with the depression score.

Conclusions: Pediatric nurses with different lengths of service in tertiary hospitals in Chengdu experience different levels of workplace stress. Pediatric nurses with 4-7 and 8-12 years of service have higher levels of workplace stress and are more likely to experience anxiety and depression.
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http://dx.doi.org/10.21037/tp-20-439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804478PMC
December 2020

Co-Benefits Analysis of Buildings Based on Different Renewal Strategies: The Emergy-Lca Approach.

Int J Environ Res Public Health 2021 01 12;18(2). Epub 2021 Jan 12.

School of Management Engineering, Shandong Jianzhu University, Jinan 250101, China.

Many cities have encountered challenges associated with rapid urban development, population growth and aging, in which urban renewal has become a promising option. Different renewal strategies, such as redevelopment, refurbishment and conservation, not only contributes to quality improvement and energy consumption reduction of dilapidated urban area, but also to greenhouse gas (GHG) emissions mitigation. Such integrated benefits are often termed as co-benefits. However, choosing the most co-benefits strategy to adopt requires a holistic understanding of social-economic and environmental aspects, which has been less reported in the existing literature. Under such circumstance, this article aims to shed light on the co-benefits of different renewal strategies by adopting the Emergy-Life cycle assessment method. Then, the method is applied to one case study of the refurbishment of an educational building located in Chongqing, China. Resource allocation, CO emissions and emergy-based indicators are calculated to assess the co-benefits during a 60-year research period, to compare the impacts of the complete demolition followed by a new one (rebuilding strategy) and the refurbishing of the existing building (refurbishment strategy). The case study shows that the annual emergy in the O&M phase of rebuilding strategy and refurbishment strategy were lower than existing building. Rebuilding and refurbishment strategies released approximately 59.1% and 80.6%, respectively, of the total CO emissions that would be produced by the existing building. The results reveal that substantial environmental benefits can be obtained in both the refurbishment and rebuilding strategies. On the other hand, it can be concluded that the emergy yield ratio (EYR) for the rebuilding strategy is higher than refurbishment strategy, which demonstrate the better performance of refurbishment considering that less resources are required to generate greater benefits. In addition, the value of environmental loading ratio (ELR) and emergy sustainability index (ESI) also suggests that the refurbishment strategy performs better from the perspective of the environment. Thereby, the refurbishment strategy is more suitable than the rebuilding strategy. Findings from this study can be useful to urban planners and decision-makers in choosing the most suitable strategy to improve the quality of existing buildings.
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http://dx.doi.org/10.3390/ijerph18020592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827707PMC
January 2021

A stepwise recognition strategy for the detection of telomerase activity via direct electrochemical analysis of metal-organic frameworks.

Analyst 2021 Mar 14;146(6):1859-1864. Epub 2021 Jan 14.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

The detection of telomerase is of great significance for monitoring cell canceration. The conventional methods depend on the extension of telomerase towards its primer to conduct signal transduction. Herein, a specific and reliable detection strategy based on stepwise recognition was developed for tandem detection of metal ions and enzymes. We first synthesized an electrically active metal-organic framework (MIL-101(Fe)), which can act directly as a signal reporter in phosphate buffered saline after being modified with capture DNA (cDNA). When the zinc ion is added as a coenzyme factor, the modified hairpin DNA on the electrode is cleaved by DNAzyme to yield the activated primer. After the addition of telomerase, the cleaved DNA strand would be extended, and the resulting sequence will be hybridized with the signal label of MIL-101(Fe)-cDNA. Therefore, a signal-on strategy for the detection of telomerase was achieved based on the direct electrochemical analysis of MIL-101(Fe). Moreover, this electrochemical biosensor can discriminate telomerase activity among different cell lines. The stepwise recognition ensured the advantages of an electrochemical biosensor such as high sensitivity and specificity during the detection process, providing a novel method for monitoring and diagnosis of diseases.
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http://dx.doi.org/10.1039/d0an02233kDOI Listing
March 2021

Broadband terahertz wave generation from an epsilon-near-zero material.

Light Sci Appl 2021 Jan 7;10(1):11. Epub 2021 Jan 7.

Center for THz Waves and College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China.

Broadband light sources emitting in the terahertz spectral range are highly desired for applications such as noninvasive imaging and spectroscopy. Conventionally, THz pulses are generated by optical rectification in bulk nonlinear crystals with millimetre thickness, with the bandwidth limited by the phase-matching condition. Here we demonstrate broadband THz emission via surface optical rectification from a simple, commercially available 19 nm-thick indium tin oxide (ITO) thin film. We show an enhancement of the generated THz signal when the pump laser is tuned around the epsilon-near-zero (ENZ) region of ITO due to the pump laser field enhancement associated with the ENZ effect. The bandwidth of the THz signal generated from the ITO film can be over 3 THz, unrestricted by the phase-matching condition. This work offers a new possibility for broadband THz generation in a subwavelength thin film made of an ENZ material, with emerging physics not found in existing nonlinear crystals.
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http://dx.doi.org/10.1038/s41377-020-00452-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790823PMC
January 2021

[Effects of different application methods of Bacillus subtilis agent on soil microbial diversity and growth of muskmelon].

Sheng Wu Gong Cheng Xue Bao 2020 Dec;36(12):2644-2656

School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, Hebei, China.

Continuous planting of muskmelon and excessive application of chemical fertilizers have caused a series of problems, such as imbalance of the soil micro-ecological environment, serious soil-borne diseases and yield loss. Application of Bacillus subtilis agent is an important way to improve soil micro-ecological environment, prevent soil-borne diseases, and promote plant growth. In this study, B. subtilis was used as experimental agent to analyze the effects of different application methods on the soil microbial diversity and growth of muskmelon in greenhouse. The number of culturable microorganisms in soil was measured by dilution-plate method. The diversity of soil uncultivated microorganisms was determined by Illumina Miseq sequencing technology. The yield of muskmelon was measured by weighing method. The number of culturable bacteria in the root irrigation, hole application and dipping root application groups was higher than that of the control in different muskmelon growth stages, but there was no significant difference among the three different application methods. The number of soil fungi from B. subtilis agent treatment groups in flowering stage was significantly lower in comparison to the control group. However, B. subtilis agent treatment did not cause significant difference on soil fungi number at the fruiting and pulling stage. Diversity analysis of uncultured microorganisms showed that the Shannon index values of bacteria were higher and Simpson index values were lower respectively in the three B. subtilis treatment groups than that in the control. Moreover, the dipping root treatment produced the lowest Shannon index value and the highest Simpson index value of fungi. NMDS and cluster analysis showed that B. subtilis agents dipping root treatment significantly affected the bacterial and fungal flora, both of which were clustered into one independent branch. The application of B. subtilis agents, especially dipping root treatment, significantly decreased the abundance of Bacteroidetes, increased the abundance of Actinobacteria and Acidobacteria. The B. subtilis agent treatment didn't produce significant effect on the diversity of fungal flora except Chytridiomycota. The height, stem diameter and leaf area of muskmelon increased by applying B. subtilis agents, and dipping root treatment produced the most significant effect. As a new type of environmental protection fertilizer, B. subtilis agent can increase the number of soil culturable microorganisms, improve soil microbial diversity, and promote growth and yield. This study would provide a scientific basis for the rational application of B. subtilis.
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http://dx.doi.org/10.13345/j.cjb.200379DOI Listing
December 2020

The Relationship Between Symptoms of Anxiety and Somatic Symptoms in Health Professionals During the Coronavirus Disease 2019 Pandemic.

Neuropsychiatr Dis Treat 2020 18;16:3153-3161. Epub 2020 Dec 18.

Department of Psychiatry, The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.

Background: The pandemic of coronavirus disease 2019 (COVID-19) has posed a threat to global health. Increasing studies have shown that the mental health status of health professionals is very poor during the COVID-19 epidemic. At present, the relationship between somatic symptoms and symptoms of anxiety of health professionals during the COVID-19 has not been reported. The purpose of this study was to explore the frequency of somatic symptoms and its related factors in health professionals with symptoms of anxiety during COVID-19 in China.

Methods: A total of 606 health professionals were assessed online with the Chinese version of the 7-item Generalized Anxiety Disorder (GAD-7) scale, 7-item Insomnia Severity Index (ISI) and the somatization subscale of Symptom Checklist 90 (SCL-90).

Results: The percentage of symptoms of anxiety, somatic symptoms and insomnia in all health professionals was 45.4%, 12.0%, and 32%, respectively. The frequency of somatic symptoms in health professionals with symptoms of anxiety was 22.9%. The SCL-90 somatization subscale score was significantly positively correlated with history of somatic diseases, GAD-7 score and ISI score in participants with symptoms of anxiety.

Conclusion: During the COVID-19, symptoms of anxiety, insomnia, and somatic symptoms are commonly observed in health professionals. Insomnia and symptoms of anxiety are independently associated with somatic symptoms of health professionals with symptoms of anxiety.
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http://dx.doi.org/10.2147/NDT.S282124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755336PMC
December 2020

Living materials fabricated via gradient mineralization of light-inducible biofilms.

Nat Chem Biol 2021 03 21;17(3):351-359. Epub 2020 Dec 21.

Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.

Living organisms have evolved sophisticated cell-mediated biomineralization mechanisms to build structurally ordered, environmentally adaptive composite materials. Despite advances in biomimetic mineralization research, it remains difficult to produce mineralized composites that integrate the structural features and 'living' attributes of their natural counterparts. Here, inspired by natural graded materials, we developed living patterned and gradient composites by coupling light-inducible bacterial biofilm formation with biomimetic hydroxyapatite (HA) mineralization. We showed that both the location and the degree of mineralization could be regulated by tailoring functional biofilm growth with spatial and biomass density control. The cells in the composites remained viable and could sense and respond to environmental signals. Additionally, the composites exhibited a maximum 15-fold increase in Young's modulus after mineralization and could be applied to repair damage in a spatially controlled manner. Beyond insights into the mechanism of formation of natural graded composites, our study provides a viable means of fabricating living composites with dynamic responsiveness and environmental adaptability.
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http://dx.doi.org/10.1038/s41589-020-00697-zDOI Listing
March 2021

Probing the tautomerization of disilenes and disilabenzenes with their isomeric silylenes: significant substituent, aromaticity and base effects.

Dalton Trans 2020 Dec 18;49(47):17341-17349. Epub 2020 Nov 18.

State Key Laboratory of Physical Chemistry of Solid Surfaces and Fujian Provincial Key Laboratory of Theoretical and Computational Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.

Disilene has attracted considerable interest due to the trans-bending geometry which is significantly different from the planar alkene. However, the equilibrium between disilene and isomeric silylsilylene has not been fully understood. Here, we report a density functional theory (DFT) study on this equilibrium. Calculations reveal significant effects of substituent, aromaticity and base. Specifically, the parent disilene is thermodynamically more stable than the isomeric silylene. When the methoxy substituent is introduced, the corresponding silylene becomes thermodynamically more stable, which could be rationalized by the Bent's rule. Interestingly, disilabenzene becomes thermodynamically more stable than the isomeric silylene when the concept of aromaticity is taken into account. Finally, once the base is introduced, the silylene could become thermodynamically more stable than the isomeric disilabenzene. The kinetic effect of the tautomerization with several typical substituents (F, Me and OMe) has also been investigated. Some species with a bridged form have been found to have a higher thermodynamic stability over the nonbridged ones. All these findings could be particularly useful to develop the chemistry of disilenes and silylenes.
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http://dx.doi.org/10.1039/d0dt03527kDOI Listing
December 2020

Compound Heterozygosity for a Novel Mutation Codon 104 (-A) (: c.313delA) and Codons 41/42 (-CTTT) (: c.126_129delCTTT) Leading to β-Thalassemia Major in a Chinese Family.

Hemoglobin 2020 Nov 16;44(6):402-405. Epub 2020 Nov 16.

Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, People's Republic of China.

β-Thalassemia (β-thal) is a hereditary blood disorder characterized by the reduced or absent synthesis of β-globin chains. Here, we report a case of severe thalassemia with compound heterozygosity for a novel deletion mutation at codon 104 (-A) (: c.313delA) and codons 41/42 (-CTTT) (: c.126_129delCTTT) on the β-globin gene (), and a coinheritance of the -α (leftward) deletion on the α-globin gene cluster. The proband was a 12-year-old boy, and four other family members were involved in this study. This novel frameshift mutation caused classical β-thal trait in the heterozygote and a transfusion-dependent form of β-thal major (β-TM) in compound heterozygosity with other β mutations.
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http://dx.doi.org/10.1080/03630269.2020.1843482DOI Listing
November 2020
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