Publications by authors named "Yuanli Zhu"

25 Publications

  • Page 1 of 1

Feasibility evaluation of amide proton transfer-weighted imaging in the parotid glands: a strategy to recognize artifacts and measure APT value.

Quant Imaging Med Surg 2021 Jun;11(6):2279-2291

Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: The feasibility and image quality of three-dimensional (3D) amide proton transfer (APT)-weighted (APTw) in parotid tumor lesions have not been well established in previous studies. This study aimed to evaluate the utility of APT imaging in parotid lesions and glands.

Methods: Patients with parotid lesions received 3D turbo spin echo (TSE) APTw on a 3.0T scanner. Two radiologists, who were blinded to the clinical data, independently evaluated the APTw image quality using 4-point Likert scales (1= poor, 4= excellent) in terms of integrity and hyperintensity artifacts. An image quality selection protocol was built based on the two scores. Evaluable images (integrity score >1) and trustable images (integrity score >3 and hyperintensity artifacts score >2) were then enrolled for APTw value comparison between parotid lesions and glands.

Results: Forty consecutive patients were included in this study. Four patients were excluded due to severe motion (n=3) or dental (n=1) artifacts, and 36 patients received the APT sequence. Among these, more parotid tumor lesions (34/36, 94.4%) than normal parotid glands (23/31, 74.2%) revealed excellent integrity scores (score =4) (P=0.034). Most parotid tumor lesions (24/34, 70.6%) and glands (16/28, 57.1%) revealed no or little hyperintensity artifacts for diagnosis (scores 3 and 4). APT values of parotid lesions and glands in the evaluable groups were 2.11%±1.15% and 1.60%±1.56%, respectively, and the difference was not significant (P=0.197). APT values of parotid lesions and glands in the trustable groups were 1.99%±1.18% and 1.03%±1.09%, respectively, and the difference was statistically significant (P=0.018).

Conclusions: 3D APTw could be used to differentiate parotid tumors and normal parotid glands; however, the technology still needs to be improved to remove artifacts. In our study, most APTw images of tumor lesions in parotid glands had acceptable image quality, and these APTw images are feasible for diagnostic use.
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http://dx.doi.org/10.21037/qims-20-675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107327PMC
June 2021

Predictive nursing helps improve treatment efficacy, treatment compliance, and quality of life in unstable angina pectoris patients.

Am J Transl Res 2021 15;13(4):3473-3479. Epub 2021 Apr 15.

The Second District of Comprehensive Internal Medicine, South Hospital of Chenzhou First People's Hospital Chenzhou 423000, Hunan Province, China.

Objective: This paper aims to explore the effects of predictive nursing on the treatment efficacy in unstable angina pectoris (UAP) patients and on their treatment compliance and quality of life (QOL).

Methods: Admitted to our hospital from November 2017 to August 2019, 110 UAP patients were recruited as the study cohort and randomized into a control group (CG) and an observation group (OG). Among them, 53 patients in the CG underwent routine nursing, and the remaining 57 patients in the OG underwent additional predictive nursing in addition to the routine nursing. The patients in both groups were observed with respect to their general data, the therapeutic effects after the nursing, their clinical indicators (length of hospital stays, stable states of angina pectoris, attacks of angina pectoris), their treatment compliance after the nursing, their negative emotions before and after the nursing, their adverse reactions during the nursing, and the changes in their blood pressure (diastolic blood pressure, systolic blood pressure) and QOL indices after the nursing.

Results: There were no differences in the general data between the two groups (P>0.05), but the clinical indicators were better in the OG (P<0.05). After the nursing, the treatment compliance and improvement of the negative emotions were better in the OG (P<0.05), and the efficacy was also better in this group (P<0.05). The incidence of adverse reactions was lower in the OG (P<0.05), and the changes in the blood pressure indices were better in this group (P<0.05). After the nursing, the QOL in the OG was better (P<0.05).

Conclusion: Predictive nursing is conducive to improving the efficacy, the treatment compliance, and the QOL of UAP patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129405PMC
April 2021

A multi-faceted, location-specific assessment of land degradation threats to peri-urban agriculture at a traditional grain base in northeastern China.

J Environ Manage 2020 Oct 2;271:111000. Epub 2020 Jul 2.

College of Earth Sciences, Jilin University, Changchun, 130061, China. Electronic address:

Urbanization-induced cultivated land degradation can hamper the ability of peri-urban agriculture (PUA) to deliver clean food and agroecosystem services. Detailed geo-information about which cultivated lands are being influenced by urbanization will be important to designing future measures for the conservation of PUA. This information will be especially relevant for traditional grain bases because PUA is often underappreciated in these regions. For this reason, we performed a multi-faceted and location-specific assessment, including soil pollution, soil fertility, basic tillage conditions and land fragmentation, of cultivated land in a rural-urban transition zone outside of a city in northeast China. We also illustrated the combined risks in different urbanized environments via GIS-based two-step spatial clustering. The results indicated that, in general, cultivated lands were more polluted and fragmented, as well as less fertile and tillable, the closer they were to the urban area. Most of the affected cultivated lands were located within 8 km of the urban periphery. Furthermore, certain urban environments exposed the surrounding cultivated lands to specific degradation in relation to different combined risks. PUA in long-standing industrial areas mainly faced risks of polluted agricultural production, underutilization and impaired landscape ecological security (LES), whereas cultivated lands close to a recently developed residential area were characterized by risks of supplying service disruption, unsustainable agricultural production, underutilization and impaired LES. The present study highlighted that PUA associated with traditional grain bases must be preserved to enhance urban sustainability and resilience, and suggests that measures which can adapt to multi-faceted local degradation issues will be the most effective protection for peri-urban areas. Furthermore, the results also suggest that multi-functional and profitable agriculture will contribute to breaking the vicious circle of land degradation in peri-urban cultivated areas of traditional grain bases.
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http://dx.doi.org/10.1016/j.jenvman.2020.111000DOI Listing
October 2020

NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis.

Cell Death Dis 2020 02 18;11(2):129. Epub 2020 Feb 18.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial hyperplasia, pannus formation, and cartilage and bone destruction. Nuclear receptor subfamily 1 group D member 1 (NR1D1) functions as a transcriptional repressor and plays a vital role in inflammatory reactions. However, whether NR1D1 is involved in synovial inflammation and joint destruction during the pathogenesis of RA is unknown. In this study, we found that NR1D1 expression was increased in synovial tissues from patients with RA and decreased in RA Fibroblast-like synoviocytes (FLSs) stimulated with IL-1β in vitro. We showed that NR1D1 activation decreased the expression of proinflammatory cytokines and matrix metalloproteinases (MMPs), while NR1D1 silencing exerted the opposite effect. Furthermore, NR1D1 activation reduced reactive oxygen species (ROS) generation and increased the production of nuclear transcription factor E2-related factor 2 (Nrf2)-associated enzymes. Mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) pathways were blocked by the NR1D1 agonist SR9009 but activated by NR1D1 silencing. NR1D1 activation also inhibited M1 macrophage polarization and suppressed osteoclastogenesis and osteoclast-related genes expression. Treatment with NR1D1 agonist SR9009 in collagen-induced arthritis (CIA) mouse significantly suppressed the hyperplasia of synovial, infiltration of inflammatory cell and destruction of cartilage and bone. Our findings demonstrate an important role for NR1D1 in RA and suggest its therapeutic potential.
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http://dx.doi.org/10.1038/s41419-020-2314-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028921PMC
February 2020

Reclamation of Cultivated Land Reserves in Northeast China: Indigenous Ecological Insecurity Underlying National Food Security.

Int J Environ Res Public Health 2020 02 13;17(4). Epub 2020 Feb 13.

College of Earth Sciences, Jilin University, Changchun 130061, China.

The competition for land resources created by the need for food security and ecological security is intensifying globally. To resolve the issue of land scarcity in agriculture following rapid urbanization, China implemented its requisition-compensation balance policy of cultivated lands in 1997, the introduction of which consumed numerous areas of land, such as river shoal and bare land, through reclamation. Moreover, these reclaimed and newly cultivated lands were mainly distributed in the northern part of China. Most previous studies of this subject have only examined the overall balance of cultivated lands in well-developed regions, and there is a lack of knowledge about the indigenous gains and losses before and after reclamation in important areas such as northeast China. Therefore, this study selected two representative county-level units in northeast China as the study area to analyze the conversion of cultivated land reserves during 1996-2015, evaluate the performance of reclaimed cultivated lands in terms of quality and productivity and calculate reclamation-induced changes in ecosystem service value. The results indicated that by 2015 only 16.02% of the original cultivated land reserves remained unconverted; nearly 60% were reclaimed as cultivated lands and over 20% were converted to other land resources. River shoal and ruderal land were the primary resources for cultivated lands compensation, and marsh, bare land and saline-alkaline land were found to be converted the most thoroughly. The gain of 23018.55 ha reclaimed cultivated lands were of relatively inferior quality and lower productivity, contributing approximately 4.32% of total grain output. However, this modest gain was at the expense of a 768.03 million yuan ecosystem services loss, with regulating services and supporting services being undermined the most. We argue that even if northeast China continues to shoulder the responsibility of compensating for a majority of cultivated land losses, it still needs to carefully process reclamation and introduce practical measures to protect indigenous ecosystems, in order to better serve the local residents and ensure prolonged food security with sustainability.
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http://dx.doi.org/10.3390/ijerph17041211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068416PMC
February 2020

Kelp cultivation effectively improves water quality and regulates phytoplankton community in a turbid, highly eutrophic bay.

Sci Total Environ 2020 Mar 18;707:135561. Epub 2019 Nov 18.

Key Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China; State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

Coastal eutrophication and its associated harmful algal blooms have emerged as one of the most severe environmental problems worldwide. Seaweed cultivation has been widely encouraged to control eutrophication and algal blooms. Among them, cultivated kelp (Saccharina japonica) dominates primarily by production and area. However, the responses of water quality and phytoplankton community to kelp farming remain unclear. Here, thirteen cruises were conducted in the kelp farms and control areas in the turbid, highly eutrophic Xiangshan Bay of the East China Sea from 2008 to 2015. Results indicated that kelp cultivation slightly increased dissolved oxygen and pH, but reduced dissolved inorganic nitrogen and phosphorus. We estimated that kelp harvesting would remove 297 t of nitrogen and 42 t of phosphorus from this bay annually. Because of decreased flow velocity, turbulence, and sediment resuspension, kelp farming greatly reduced suspended solids and increased transparency, resulting in increases in phytoplankton chlorophyll a and abundance. Additionally, kelp farming appreciably increased phytoplankton species number, Marglef richness, and Shannon-Wiener diversity indices by 51.6%, 40.1%, and 13.1%, respectively. Analysis of similarity and similarity percentages demonstrated that phytoplankton community composition differed significantly between the farm and control area, which was mostly attributed to long-chained diatoms and single-celled dinoflagellates. However, after the kelp harvesting, all measurements of water quality and phytoplankton biomass, diversity, and community composition exhibited no significant difference. Our study highlights that kelp cultivation alleviates eutrophication and acidification and enhances phytoplankton diversity, thus providing guidance for macroalgal aquaculture and remediation in eutrophic waters.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135561DOI Listing
March 2020

Phytoplankton biomass and size structure in Xiangshan Bay, China: Current state and historical comparison under accelerated eutrophication and warming.

Mar Pollut Bull 2019 May 20;142:119-128. Epub 2019 Mar 20.

Key Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China; State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

To explore the effects of coastal eutrophication and warming on phytoplankton biomass and cell size, we analyzed current and historical data for size-fractionated chlorophyll a (chla) in Xiangshan Bay, China. Results showed that micro- and nanophytoplankton overwhelmingly dominated (>84%) in all seasons. The contribution of micro-chla was significantly lower in warm than in cold seasons, whereas contribution of pico-chla showed the opposite result. Overall, the micro-chla contribution increased with decreasing pico-chla contribution from the stable, clear, eutrophic upper bay to the turbulent, turbid lower bay, indicating that phytoplankton size structure on a spatial scale was largely shaped by water column stability and light rather than by nutrients. Since the 1980s, phytoplankton biomass, primary productivity, and micro-chla contribution in Xiangshan Bay have increased sharply with increasing nutrient amounts and temperature. Additionally, it seems that algal bloom seasonality has shifted forward from spring to winter since the power plant operations in 2006.
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http://dx.doi.org/10.1016/j.marpolbul.2019.03.013DOI Listing
May 2019

miR-148b-3p functions as a tumor suppressor in GISTs by directly targeting KIT.

Cell Commun Signal 2018 04 16;16(1):16. Epub 2018 Apr 16.

Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Dadao, Wuhan, 430030, People's Republic of China.

Background: Gain-of-function mutations and overexpression of KIT are characteristic features of gastrointestinal stromal tumor (GIST). Dysregulation in miRNA expression may lead to KIT overexpression and tumorigenesis.

Methods: miRNA microarray analysis and real-time PCR were used to determine the miRNA expression profiles in a cohort of 69 clinical samples including 50 CD117/KIT GISTs and 19 CD117/wild-type GISTs. GO enrichment and KEGG pathway analyses were performed to reveal the predicted targets of the dysregulated miRNAs. Of the dysregulated miRNAs whose expression was inversely correlated with that of KIT miRNAs were predicted by bioinformatics analysis and confirmed by luciferase reporter assay. Cell counting kit-8 (CCK-8) and flow cytometry were used to measure the cell proliferation, cycle arrest and apoptosis. Wound healing and transwell assays were used to evaluate migration and invasion. A xenograft BALB/c nude mouse model was applied to investigate the tumorigenesis in vivo. Western blot and qRT-PCR were used to investigate the protein and mRNA levels of KIT and its downstream effectors including ERK, AKT and STAT3.

Results: Of the six miRNAs whose expression was inversely correlated with that of KIT, we found that miR-148b-3p was significantly downregulated in the CD117/KIT GIST cohort. This miRNA was subsequently found to inhibit proliferation, migration and invasion of GIST882 cells. Mechanistically, miR-148b-3p was shown to regulate KIT expression through directly binding to the 3'-UTR of the KIT mRNA. Restoration of miR-148b-3p expression in GIST882 cells led to reduced expression of KIT and the downstream effectors proteins ERK, AKT and STAT3. However, overexpression of KIT reversed the inhibitory effect of miR-148b-3p on cell proliferation, migration and invasion. Furthermore, we found that reduced miR-148b-3p expression correlated with poor overall survival (OS) and disease-free survival (DFS) in GIST patients.

Conclusion: miR-148b-3p functions as an important regulator of KIT expression and a potential prognostic biomarker for GISTs.
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http://dx.doi.org/10.1186/s12964-018-0228-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902930PMC
April 2018

Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Activity.

Front Pharmacol 2017 19;8:928. Epub 2017 Dec 19.

Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Dihydromyricetin (DMY), the main flavonoid component of , possesses pharmacological activities useful for treatment of diseases associated with inflammation and oxidative damage. Because osteoclasts are often involved in chronic low-grade systemic inflammation and oxidative damage, we hypothesized that DMY may be an effective treatment for osteoclast-related diseases. The effects of DMY on osteoclast formation and activity were examined . Female C57BL/6 mice were ovariectomized to mimic menopause-induced bone loss and treated with DMY, and femur samples were subjected to bone structure and histological analysis, serum biochemical indicators were also measured. DMY suppressed the activation of nuclear factor-κB, c-Fos and mitogen-activated protein kinase, and prevented production of reactive oxygen species. DMY decreased expression of osteoclast-specific genes, including , and . In addition, DMY prevented bone loss and decreased serum levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6, and with a decrease in the ratio between receptor activator of nuclear factor-κB (RANK) ligand (RANKL) and osteoprotegerin (OPG) . These findings demonstrate that DMY attenuates bone loss and inhibits osteoclast formation and activity through modulation of multiple pathways both upstream and downstream of RANKL signaling. DMY may thus be a useful option for treatment of osteoclast-related diseases such as rheumatoid arthritis and osteoporosis.
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http://dx.doi.org/10.3389/fphar.2017.00928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742133PMC
December 2017

Aesthetic and hearing rehabilitation in patients with bilateral microtia-atresia.

Int J Pediatr Otorhinolaryngol 2017 Oct 10;101:150-157. Epub 2017 Aug 10.

Department of Otolaryngology, Peking Union Medical College Hospital, Beijing, PR China. Electronic address:

Objectives: To evaluate the safety and efficacy of auricle reconstruction and active transcutaneous bone-conduction implantation in patients with bilateral microtia-atresia.

Design: Patients were chosen prospectively, with each being his/her own control.

Setting: The setting was a tertiary referral center.

Participants: Twelve patients, aged 6-18 years, with bilateral microtia-atresia suffering from bilateral conductive hearing loss. All had an upper bone conduction threshold limit of 45 dB HL at frequencies of 0.5-4 kHz.

Main Outcome Measures: Patient satisfaction with the reconstructed auricle was rated as highly satisfactory, basically satisfactory, or unsatisfactory. Mean pure-tone thresholds and speech audiometry test results were compared among patients unaided, with a soft-band Bonebridge, and with an implanted Bonebridge. Subjective satisfaction was analyzed using three questionnaires: the Abbreviated Profile of Hearing Aid Benefit (APHAB), the Glasgow children's benefit inventory (GCBI), and the International Outcome Inventory for Hearing Aids (IOI-HA).

Results: All patients who underwent auricle reconstruction expressed satisfaction with their appearance. The mean pure-tone thresholds of unaided patients and those with soft-band and implanted Bonebridge were 55.25 ± 3.43 dBHL, 31.37 ± 3.03 dBHL, and 21.25 ± 2.16 dBHL, respectively. The mean speech discrimination scores measured in a sound field with a presentation level of 65 dB SPL under these three conditions were 46.0 ± 0.11%, 80.0 ± 0.09%, and 94.0 ± 0.02%, respectively. Questionnaires demonstrated patients' benefits and satisfaction with this surgery.

Conclusions: The surgical procedure involving auricle reconstruction and Bonebridge implantation was safe and effective for patients with bilateral microtia-atresia, solving both appearance and hearing problems.
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http://dx.doi.org/10.1016/j.ijporl.2017.08.008DOI Listing
October 2017

Head and neck angiography at 70 kVp with a third-generation dual-source CT system in patients: comparison with 100 kVp.

Neuroradiology 2017 Nov 24;59(11):1071-1081. Epub 2017 Aug 24.

Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No.1 Shuai Fu Yuan, Dong Cheng District, Beijing, 100730, China.

Purpose: This study was conducted in order to evaluate the image quality of 70 kVp and 25 mL contrast medium (CM) volume for head and neck computed tomographic angiography (CTA) and assess the diagnostic accuracy for arterial stenosis.

Methods: Fifty patients were prospectively divided into two groups randomly: group A (n = 25), 70 kVp with 25 mL CM, and group B (n = 25), 100 kVp with 40 mL CM. CT attenuation values, noise, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) of the shoulder, neck, and cerebral arteries were measured for objective image quality. Subjective image quality of the shoulder and cerebral arteries was also evaluated. For patients undergoing digital subtracted angiography (DSA), diagnostic accuracy of CTA was assessed with DSA as reference standard.

Results: The SNRs of the shoulder, neck, and cerebral arteries in group A were higher than those in group B (P < 0.05). The CNRs of the shoulder and neck arteries in group A were higher than those in group B (P < 0.05). There was no significant difference in subjective image quality of arteries between group A and group B (P > 0.05). The accuracy was noted as 94.0% (156/166) in group A and 97.1% (134/138) in group B for ≥ 50% stenosis. The accuracy of intracranial arterial stenosis was lower than that of extracranial arterial stenosis in group A. The radiation dose of group A was significantly decreased by 56% than that of group B.

Conclusion: Head and neck CTA at 70 kVp using 25 mL CM can obtain diagnostic image quality with lower radiation dose while maintaining high accuracy in detecting the arterial stenosis compared with the 100-kVp and 40-mL CM.
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http://dx.doi.org/10.1007/s00234-017-1901-4DOI Listing
November 2017

Co-targeting of IGF1R/mTOR pathway by miR-497 and miR-99a impairs hepatocellular carcinoma development.

Oncotarget 2017 Jul;8(29):47984-47997

Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China.

Persistent activation of IGF1R/mTOR signaling pathway plays crucial role in the development of hepatocellular carcinoma (HCC). Therefore, our goal was to elucidate microRNAs (miRNAs) targeting IGF1R/mTOR and the therapeutic potential of single or dual miRNA on HCC development. In this study, we found that miR-497 and miR-99a that target the 3'-UTR of both IGF1R and mTOR were down-regulated in HCC human tissues and cell lines. Functional assay revealed that ectopic expression of miR-497 or miR-99a in HCC cells resulted in a significant inhibition on tumor growth and invasiveness in vitro and tumor development in vivo via repressing the expression of IGF1R and mTOR. Such inhibitory effect on tumor growth is reversed by application of IGF1 ((IGF1R ligand) or MHY1485 (mTOR agonist) in vitro. Furthermore, we found that simultaneous over-expression of both miR-497 and miR-99a exhibited much stronger inhibitory effects on tumor growth than their individual effect, which is still correlated with significantly stronger repression of IGF1R and mTOR. Overall, our results suggest that miR-497 and miR-99a both function as tumor-suppressive miRNAs by suppressing IGF1R/mTOR signaling pathway. The synergistic actions of these two miRNAs partly correlated with IGF1R and mTOR levels, which may represent new strategies for the molecular treatment of HCC.
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http://dx.doi.org/10.18632/oncotarget.18207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564620PMC
July 2017

Impacts from Land Use Pattern on Spatial Distribution of Cultivated Soil Heavy Metal Pollution in Typical Rural-Urban Fringe of Northeast China.

Int J Environ Res Public Health 2017 03 22;14(3). Epub 2017 Mar 22.

College of Earth Sciences, Jilin University, Changchun 130061, China.

Under rapid urban sprawl in Northeast China, land conversions are not only encroaching on the quantity of cultivated lands, but also posing a great threat to black soil conservation and food security. This study's aim is to explore the spatial relationship between comprehensive cultivated soil heavy metal pollution and peri-urban land use patterns in the black soil region. We applied spatial lag regression to analyze the relationship between PLI (pollution load index) and influencing factors of land use by taking suburban cultivated land of Changchun Kuancheng District as an empirical case. The results indicate the following: (1) Similar spatial distribution characteristics are detected between Pb, Cu, and Zn, between Cr and Ni, and between Hg and Cd. The Yitong River catchment in the central region, and the residential community of Lanjia County in the west, are the main hotspots for eight heavy metals and PLI. Beihu Wetland Park, with a larger-area distribution of ecological land in the southeast, has low level for both heavy metal concentrations and PLI values. Spatial distribution characteristics of cultivated heavy metals are related to types of surrounding land use and industry; (2) Spatial lag regression has a better fit for PLI than the ordinary least squares regression. The regression results indicate the inverse relationship between heavy metal pollution degree and distance from long-standing residential land and surface water. Following rapid urban land expansion and a longer accumulation period, residential land sprawl is going to threaten cultivated land with heavy metal pollution in the suburban black soil region, and cultivated land irrigated with urban river water in the suburbs will have a higher tendency for heavy metal pollution.
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http://dx.doi.org/10.3390/ijerph14030336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369171PMC
March 2017

Application of Third-generation Dual-source CT for Ultra-high Resolution Temporal Bone Imaging Using Automated Tube Voltage Adaptation in Children with Hearing Impairment.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2017 Feb;39(1):12-16

Department of Radiology, CAMS and PUMC,Beijing 100730,China.

Objective To evaluate the radiation dose and image quality of the third-generation dual-source CT using automated tube voltage adaptation (CARE kV) in temporal bone scanning in pediatric patients with hearing impairment. Methods Totally 27 children with hearing impairment less than 18 years old were randomly divided into two groups:Group A (n=14),examined with CARE kV (reference with 100 kV and 214 mA)and sinogram-affirmed iterative reconstruction;and Group B (n=13),examined with CARE kV (reference with 100 kV and 171 mA)and advanced modeled iterative reconstruction. The scan range was from eyebrow to the end of the mastoid process. CT values and image noise were measured.The signal to noise ratio (SNR)was calculated. Subjective image quality was assessed by two radiologists and later in a consensus reading. Results There was no significant difference in CT value,image noise,and SNR between these two groups (all P>0.05). Also,the subjective scores of the 10 anatomical structures showed no significant difference (all P>0.05). The CT dose index volume and dose-length product were (11.62+1.92)mGy and (106.92+37.48)mGy·cm,respectively,in group B and (21.28+2.19)mGy (t=12.15,P<0.001)and (229.65+56.26)mGy·cm (t=6.62,P<0.001)in group A,decreased by 45% and 53%. Conclusion Compared with the second-generation dual-source CT,the third-generation dual-source CT for the scanning of temporal bone with CARE kV can ensure image quality and reduce radiation dose.
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http://dx.doi.org/10.3881/j.issn.1000-503X.2017.01.003DOI Listing
February 2017

Third-generation Dual-source CT for Head and Neck CT Angiography with 70 kV Tube Voltage and 20-25 ml Contrast Medium in Patients With Body Weight Lower than 75 kg.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2017 Feb;39(1):4-8

Department of Radiology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.

Objective To investigate the image quality of head and neck CT angiography (CTA)using the third-generation dual-source CT combined with 70 kV tube voltage and 20-25 ml contrast medium (CM),and evaluate the effects of venous artifacts arising from the CM on the ipsilateral side of injection. Methods Totally 40 consecutive patients with suspected vascular diseases and body weight lower than 75 kg prospectively underwent head and neck CTA examination using the third-generation dual-source CT. CTA was performed with a third-generation dual-source CT system. Patients were randomly divived into 70 kV group (n=20)and 100 kV group (n=20). The 70 kV group used 20-25 ml CM and advanced modeled iterative reconstruction technique,and the 100 kV group used 40 ml CM and filtered back projection. Venous artifacts and CM residues were evaluated by a 3-point scale (1=excellent,3=poor),respectively. Results The effective dose of 70 kV group decreased 58% compared to 100 kV group (t=-18.14,P<0.001).In the 70 kV group,16 patients (80.0%)presented with venous artifacts and six of them (37.5%,6/16)affected the adjacent arteries. In the 100 kV group,19 patients (95.0%)presented with venous artifacts,and seven of them (36.8%,7/19)affected the adjacent arteries (Z=-0.878,P=0.380). In the 70 kV group,13 patients (65.0%)presented with obvious CM residues and two of them (15.3%,2/13)prolonged into the superior vena cava (SVC). In the 100 kV group,19 patients(95.0%)presented with obvious CM residues,and thirteen of them(68.4%,13/19)prolonged into the SVC (Z=-3.654,P<0.001). Conclusion Compared with the 100 kV,the third-generation dual-source CT for head and neck CTA,combined with 70 kV and 20-25 ml CM,can remarkably decrease the radiation dose,along with reduced CM residues and comparable venous artifacts.
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http://dx.doi.org/10.3881/j.issn.1000-503X.2017.01.002DOI Listing
February 2017

Statistical approach for the retrieval of phytoplankton community structures from in situ fluorescence measurements.

Opt Express 2016 Oct;24(21):23635-23653

Knowledge of phytoplankton community structures is important to the understanding of various marine biogeochemical processes and ecosystem. Fluorescence excitation spectra (F(λ)) provide great potential for studying phytoplankton communities because their spectral variability depends on changes in the pigment compositions related to distinct phytoplankton groups. Commercial spectrofluorometers have been developed to analyze phytoplankton communities by measuring the field F(λ), but estimations using the default methods are not always accurate because of their strong dependence on norm spectra, which are obtained by culturing pure algae of a given group and are assumed to be constant. In this study, we proposed a novel approach for estimating the chlorophyll a (Chl a) fractions of brown algae, cyanobacteria, green algae and cryptophytes based on a data set collected in the East China Sea (ECS) and the Tsushima Strait (TS), with concurrent measurements of in vivo F(λ) and phytoplankton communities derived from pigments analysis. The new approach blends various statistical features by computing the band ratios and continuum-removed spectra of F(λ) without requiring a priori knowledge of the norm spectra. The model evaluations indicate that our approach yields good estimations of the Chl a fractions, with root-mean-square errors of 0.117, 0.078, 0.072 and 0.060 for brown algae, cyanobacteria, green algae and cryptophytes, respectively. The statistical analysis shows that the models are generally robust to uncertainty in F(λ). We recommend using a site-specific model for more accurate estimations. To develop a site-specific model in the ECS and TS, approximately 26 samples are sufficient for using our approach, but this conclusion needs to be validated in additional regions. Overall, our approach provides a useful technical basis for estimating phytoplankton communities from measurements of F(λ).
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http://dx.doi.org/10.1364/OE.24.023635DOI Listing
October 2016

miR-497-5p inhibits cell proliferation and invasion by targeting KCa3.1 in angiosarcoma.

Oncotarget 2016 09;7(36):58148-58161

Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Angiosarcoma is a rare malignant mesenchymal tumor with poor prognosis. We aimed to identify malignancy-associated miRNAs and their target genes, and explore biological functions of miRNA and its target in angiosarcoma. By miRNA microarrays and reverse transcription polymerase chain reaction, we identified 1 up-regulated miRNA (miR-222-3p) and 3 down-regulated miRNAs (miR-497-5p, miR-378-3p and miR-483-5p) in human angiosarcomas compared with human capillary hemangiomas. The intermediate-conductance calcium activated potassium channel KCa3.1 was one of the putative target genes of miR-497-5p, and marked up-regulation of KCa3.1 was detected in angiosarcoma biopsy specimens by immunohistochemistry. The inverse correlation of miR-497-5p and KCa3.1 also was observed in the ISO-HAS angiosarcoma cell line at the mRNA and protein levels. The direct targeting of KCa3.1 by miR-497-5p was evidenced by reduced luciferase activity due to complementary binding of miR-497-5p to KCa3.1 mRNA 3' untranslated region. For the functional role of miR-497-5p/KCa3.1 pair, we showed that application of TRAM-34, a specific KCa3.1 channel blocker, or transfection of ISO-HAS cells with KCa3.1 siRNA or miR-497-5p mimics inhibited cell proliferation, cell cycle progression, and invasion by down-regulating cell-cycle related proteins including cyclin D1, surviving and P53 and down-regulating matrix metallopeptidase 9. In an in vivo angiosarcoma xenograft model, TRAM-34 or miR-497-5p mimics both inhibited tumor growth. In conclusion, the tumor suppressor miR-497-5p down-regulates KCa3.1 expression and contributes to the inhibition of angiosarcoma malignancy development. The miR-497-5p or KCa3.1 might be potential new targets for angiosarcoma treatment.
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http://dx.doi.org/10.18632/oncotarget.11252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295420PMC
September 2016

A Neural Network Model for K(λ) Retrieval and Application to Global Kpar Monitoring.

PLoS One 2015 17;10(6):e0127514. Epub 2015 Jun 17.

College of Mining Engineering, Hebei United University, Tangshan 063009, China.

Accurate estimation of diffuse attenuation coefficients in the visible wavelengths Kd(λ) from remotely sensed data is particularly challenging in global oceanic and coastal waters. The objectives of the present study are to evaluate the applicability of a semi-analytical Kd(λ) retrieval model (SAKM) and Jamet's neural network model (JNNM), and then develop a new neural network Kd(λ) retrieval model (NNKM). Based on the comparison of Kd(λ) predicted by these models with in situ measurements taken from the global oceanic and coastal waters, all of the NNKM, SAKM, and JNNM models work well in Kd(λ) retrievals, but the NNKM model works more stable and accurate than both SAKM and JNNM models. The near-infrared band-based and shortwave infrared band-based combined model is used to remove the atmospheric effects on MODIS data. The Kd(λ) data was determined from the atmospheric corrected MODIS data using the NNKM, JNNM, and SAKM models. The results show that the NNKM model produces <30% uncertainty in deriving Kd(λ) from global oceanic and coastal waters, which is 4.88-17.18% more accurate than SAKM and JNNM models. Furthermore, we employ an empirical approach to calculate Kpar from the NNKM model-derived diffuse attenuation coefficient at visible bands (443, 488, 555, and 667 nm). The results show that our model presents a satisfactory performance in deriving Kpar from the global oceanic and coastal waters with 20.2% uncertainty. The Kpar are quantified from MODIS data atmospheric correction using our model. Comparing with field measurements, our model produces ~31.0% uncertainty in deriving Kpar from Bohai Sea. Finally, the applicability of our model for general oceanographic studies is briefly illuminated by applying it to climatological monthly mean remote sensing reflectance for time ranging from July, 2002- July 2014 at the global scale. The results indicate that the high Kd(λ) and Kpar values are usually found around the coastal zones in the high latitude regions, while low Kd(λ) and Kpar values are usually found in the open oceans around the low-latitude regions. These results could improve our knowledge about the light field under waters at either the global or basin scales, and be potentially used into general circulation models to estimate the heat flux between atmosphere and ocean.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127514PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471105PMC
March 2016

Remote estimation of phytoplankton size fractions using the spectral shape of light absorption.

Opt Express 2015 Apr;23(8):10301-18

Phytoplankton size structure plays an important role in ocean biogeochemical processes. The light absorption spectra of phytoplankton provide a great potential for retrieving phytoplankton size structure because of the strong dependence on the packaging effect caused by phytoplankton cell size and on different pigment compositions related to phytoplankton taxonomy. In this study, we investigated the variability in light absorption spectra of phytoplankton in relation to the size structure. Based on this, a new approach was proposed for estimating phytoplankton size fractions. Our approach use the spectral shape of the normalized phytoplankton absorption coefficient (a(ph)(λ)) through principal component analysis (PCA). Values of a(ph)(λ) were normalized to remove biomass effects, and PCA was conducted to separate the spectral variance of normalized a(ph)(λ) into uncorrelated principal components (PCs). Spectral variations captured by the first four PC modes were used to build relationships with phytoplankton size fractions. The results showed that PCA had powerful ability to capture spectral variations in normalized a(ph)(λ), which were significantly related to phytoplankton size fractions. For both hyperspectral a(ph)(λ) and multiband a(ph)(λ), our approach is applicable. We evaluated our approach using wide in situ data collected from coastal waters and the global ocean, and the results demonstrated a good and robust performance in estimating phytoplankton size fractions in various regions. The model performance was further evaluated by a(ph)(λ) derived from in situ remote sensing reflectance (R(rs)(λ)) with a quasi-analytical algorithm. Using R(rs)(λ) only at six bands, accurate estimations of phytoplankton size fractions were obtained, with R(2) values of 0.85, 0.61, and 0.76, and root mean-square errors of 0.130, 0.126, and 0.112 for micro-, nano-, and picophytoplankton, respectively. Our approach provides practical basis for remote estimation of phytoplankton size structure using a(ph)(λ) derived from satellite observations or rapid field instrument measurements in the future.
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http://dx.doi.org/10.1364/OE.23.010301DOI Listing
April 2015

Amiloride inhibits osteoclastogenesis by suppressing nuclear factor-κB and mitogen-activated protein kinase activity in receptor activator of nuclear factor-κB-induced RAW264.7 cells.

Mol Med Rep 2015 May 15;11(5):3451-6. Epub 2015 Jan 15.

Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Amiloride is widely used in clinical practice as a diuretic and is known to interact with the epithelial sodium channel and acid‑sensing ion channel proteins, as well as Na+/H+ antiporters and Na+/Ca2+ exchangers. The aim of the present study was to examine the effects of amiloride on receptor activator of nuclear factor‑κB ligand (RANKL)‑induced osteoclastogenesis and to elucidate the underlying mechanisms in the RAW264.7 murine macrophage cell line. The number of tartrate‑resistant acid phosphatase (TRAP)‑positive multinucleated cells were counted and the bone resorption area was estimated. In addition the expression levels of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) mRNA and osteoclast‑specific genes, including TRAP, matrix metalloproteinase 9, cathepsin K and osteoclast‑associated receptor, were examined using reverse transcription‑quantitative polymerase chain reaction. The nuclear factor‑κB (NF‑κB) and mitogen‑activated protein kinase (MAPK) signaling pathways were also investigated using western blotting. The results showed that amiloride significantly reduced the number of TRAP‑positive multinucleated cells as well as the bone resorption area. Amiloride also downregulated the expression of NFATc1 mRNA and inhibited the expression of osteoclast‑specific genes. A possible underlying mechanism may be that amiloride suppresses the degradation of the inhibitor of NF‑κB and blocks the activation of c‑Jun N‑terminal kinase, extracellular signal‑regulated kinase and p38, thus implicating the NF‑κB and MAPK pathway is this process. In conclusion, the current data suggest that amiloride is a strong inhibitor of osteoclast differentiation, indicating a novel indication for amiloride in the treatment of bone‑loss‑related diseases.
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http://dx.doi.org/10.3892/mmr.2015.3204DOI Listing
May 2015

Evaluation of spatial-temporal variations and trends in surface water quality across a rural-suburban-urban interface.

Environ Sci Pollut Res Int 2014 25;21(13):8036-51. Epub 2014 Mar 25.

Water quality degradation is often a severe consequence of rapid economic expansion in developing countries. Methods to assess spatial-temporal patterns and trends in water quality are essential for guiding adaptive management efforts aimed at water quality remediation. Temporal and spatial patterns of surface water quality were investigated for 54 monitoring sites in the Wen-Rui Tang River watershed of eastern China to identify such patterns in water quality occurring across a rural-suburban-urban interface. Twenty physical and chemical water quality parameters were analyzed in surface waters collected once every 4-8 weeks from 2000 to 2010. Temporal and spatial variations among water quality parameters were assessed between seasons (wet/dry) and among major land use zones (urban/suburban/rural). Factor analysis was used to identify parameters that were important in assessing seasonal and spatial variations in water quality. Results revealed that parameters related to organic pollutants (dissolved oxygen (DO), chemical oxygen demand (manganese) (COD(Mn)), and 5-day biochemical oxygen demand (BOD₅)), nutrients (ammonia nitrogen (NH₄ ⁺-N), total nitrogen (TN), total phosphorus (TP)), and salt concentration (electrical conductivity (EC)) were the most important parameters contributing to water quality variation. Collectively, they explained 70.9 % of the total variance. A trend study using the seasonal Kendall test revealed reductions in COD(Mn), BOD₅, NH₄ ⁺-N, petrol, V-phen, and EC concentrations over the 11-year study period. Cluster analysis was employed to evaluate variation among 14 sampling sites representative of dominant land use categories and indicated three, three, and four clusters based on organic, nutrient, and salt water quality characteristics, respectively. Factors that are typically responsible for water quality degradation (including population, topography, and land use) showed no strong correlation with water quality trends implying considerable point source inputs in the watershed. The results of this study help inform ongoing water quality remediation efforts by documenting trends in water quality across various land use zones.
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http://dx.doi.org/10.1007/s11356-014-2716-zDOI Listing
May 2015

Synthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for bone regeneration.

J Huazhong Univ Sci Technolog Med Sci 2012 Aug 11;32(4):563-570. Epub 2012 Aug 11.

Department of Orthopaedics, Huazhong University of Science and Technology, Wuhan, 430030, China.

A novel unsaturated polyphosphoester (UPPE) was devised in our previous research, which is a kind of promising scaffold for improving bone regeneration. However, the polymerization process of UPPE scaffolds was unfavorable, which may adversely affect the bioactivity of osteoinductive molecules added if necessary, such as recombinant human bone morphogenetic protein-2 (rhBMP2). The purpose of this study was to build a kind of optimal scaffold named UPPE-PLGA-rhBMP2 (UPB) and to investigate the bioactivity of rhBMP2 in this scaffold. Furthermore, the cytotoxicity and biocompatibility of UPB scaffold was assessed in vitro. A W1/O/W2 method was used to fabricate PLGA-rhBMP2 microspheres, and then the microspheres were added to UPPE for synthesizing UPB scaffold. The morphological characters of PLGA-rhBMP2 microspheres and UPB scaffolds were observed under the scanning electron microscopy and laser scanning confocal microscopy. The cumulative release of UPB scaffolds was detected by using ELISA. The cytotoxicity and biocompatibility of UPB scaffolds were evaluated through examining the adsorption and apoptosis of bone marrow stromal cells (bMSCs) seeded on the surface of UPB scaffolds. The bioactivity of rhBMP2 in UPB scaffolds was assessed through measuring the alkaline phosphates (ALP) activity in bMSCs seeded. The results showed that UPB scaffolds sequentially exhibited burst and sustained release of rhBMP2. The cytotoxicity was greatly reduced when the scaffolds were immersed in buffer solution for 2 h. bMSCs attached and grew on the surface of soaked UPB scaffolds, exerting well biocompatibility. The ALP activity of bMSCs seeded was significantly enhanced, indicating that the bioactivity of rhBMP2 remained and still took effect after the unfavorable polymerization process of scaffolds. It was concluded that UPB scaffolds have low cytotoxicity, good biocompatibility and preserve bioactivity of rhBMP2. UPB scaffolds are promising in improving bone regeneration.
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http://dx.doi.org/10.1007/s11596-012-0097-4DOI Listing
August 2012

Optimizing water quality monitoring networks using continuous longitudinal monitoring data: a case study of Wen-Rui Tang River, Wenzhou, China.

J Environ Monit 2011 Oct 13;13(10):2755-62. Epub 2011 Sep 13.

The Environmental Geographic Information System Laboratory, School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou, 325035, China.

Identification of representative sampling sites is a critical issue in establishing an effective water quality monitoring program. This is especially important at the urban-agriculture interface where water quality conditions can change rapidly over short distances. The objective of this research was to optimize the spatial allocation of discrete monitoring sites for synoptic water quality monitoring through analysis of continuous longitudinal monitoring data collected by attaching a water quality sonde and GPS to a boat. Sampling was conducted six times from March to October 2009 along a 6.5 km segment of the Wen-Rui Tang River in eastern China that represented an urban-agricultural interface. When travelling at a velocity of ∼2.4 km h(-1), this resulted in water quality measurements at ∼20 m interval. Ammonia nitrogen (NH(4)(+)-N), electrical conductivity (EC), dissolved oxygen (DO), and turbidity data were collected and analyzed using Cluster Analysis (CA) to identify optimal locations for establishment of long-term monitoring sites. The analysis identified two distinct water quality segments for NH(4)(+)-N and EC and three distinct segments for DO and turbidity. According to our research results, the current fixed-location sampling sites should be adjusted to more effectively capture the distinct differences in the spatial distribution of water quality conditions. In addition, this methodology identified river reaches that require more comprehensive study of the factors leading to the changes in water quality within the identified river segment. The study demonstrates that continuous longitudinal monitoring can be a highly effective method for optimizing monitoring site locations for water quality studies.
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http://dx.doi.org/10.1039/c1em10352kDOI Listing
October 2011

Hyperhomocysteinemia inhibited cardiac stem cell homing into the peri-infarcted area post myocardial infarction in rats.

Exp Mol Pathol 2011 Aug 1;91(1):411-8. Epub 2011 May 1.

Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.

Background: Hyperhomocysteinemia (HHcy) has been reported as an independent risk factor for coronary artery disease; however it is not clear regarding the action of HHcy on the homing of cardiac stem cells (CSCs) to the damaged myocardium and the consequent CSCs-mediated cardiac repair post myocardial infarction.

Methods: Sprague-Dawley (SD) rats were divided into 4 groups. HHcy was induced in the rats by a 6-week high-methionine diet. Rat heart MI model was developed by left coronary artery ligation. Immunofluorescence was used to examine the CSCs migration in vivo via injecting BrdU-labeled CSCs into AV-groove followed by a coronary ligation. Immunohistochemistry, western blot and ELISA analysis were carried out to detect the expression of stem cell factor (SCF) protein, and RT-PCR was conducted for the expression of SCF mRNA.

Results: On day 5 of MI model creation, accumulation of CSCs was significantly increased in the peri-infarcted area by the non-hyperhomocysteinemic rats, which led to an improvement of cardiac function at 3 weeks after MI. however, the accumulation of CSCs was markedly decreased by the hyperhomocysteinemic rats followed with the decline of cardiac function. SCF expression was also significantly decreased in the peri-infarcted area by the hyperhomocysteinemic rats compared to the non-hyperhomocysteinemic rats. The experiments in vitro confirmed that homocysteine (Hcy) decreased SCF expression via inhibition of TNF-α-induced activity of NF-κB, further reduced the migration of CSCs.

Conclusion: It demonstrated that hyperhomocysteinemia may significantly contribute to restrain CSCs-mediated cardiac repair by reducing SCF-induced homing of CSCs.
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http://dx.doi.org/10.1016/j.yexmp.2011.04.010DOI Listing
August 2011
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