Publications by authors named "Yuanjun Li"

35 Publications

Polymicrobial keratitis after accelerated corneal collagen cross-linking in keratoconus: Case reports and literature review.

Eur J Ophthalmol 2021 Nov 25:11206721211051922. Epub 2021 Nov 25.

159374Eye Center of Xiangya Hospital, Central South University, Changsha, China.

Purpose: To report two cases of polymicrobial keratitis following corneal collagen cross-linking for keratoconus and to review the literature.

Methods: Retrospective case note and literature review.

Results: The first case involved a 27-year-old male who presented with amebic corneal ulcers 3 days after the collagen cross-linking procedure. Some gram-negative (gram-ve) cocci were found upon staining, and cysts were observed by confocal microscopy at 7 days after surgery. infection mixed with gram-ve organisms was diagnosed. In the second case, a 14-year male developed corneal infection with anterior chamber empyema 3 days after the collagen cross-linking procedure for keratoconus. Occasional gram-positive (gram + ve) cocci and gram-ve bacilli were observed under a microscope. The mixed keratitis in the two patients resolved after systemic and topical antibiotic therapy, but the infection ultimately resulted in corneal scarring. Follow-up keratoplasty was needed to improve vision acuity in both patients.

Conclusion: Although ultraviolet irradiation and the reactive oxygen released by riboflavin during collagen cross-linking have bactericidal effects, a lack of a corneal epithelial barrier, bandage contact lens usage, perioperative hygiene, and an abnormal immune state are risk factors for infectious keratitis after collagen cross-linking. Perioperative management of collagen cross-linking is important to prevent infection.
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http://dx.doi.org/10.1177/11206721211051922DOI Listing
November 2021

[Analysis of metabolite differences in skin between Clapp's Favorite and its mutant Red Clapp's Favorite through non-targeted metabolomics].

Se Pu 2021 Nov;39(11):1203-1212

Yantai Academy of Agricultural Sciences Shandong Province, Yantai 26550, China.

Red Clapp's Favorite is the red mutation cultivar of the pear cultivar Clapp's Favorite. Fruit color is an important feature of pear fruits, with red skin generally attracting consumers. Anthocyanin, chlorophyll, and carotenoids are the most important pigments in the color formation of fruits. The red color of pear skin is mainly due to the concentration and composition of anthocyanin. Metabolomics is an emerging discipline that focuses on the qualitative and quantitative analysis of small metabolites with low molecular weight in biological cells and tissues. As an important part of systems biology, it is an effective means to solve many complex biological problems. Studies have analyzed pigment content, composition, and differentially expressed genes in the skin of green and red pears from various aspects. Anthocyanins are responsible for physiological activity on regulating pathways. The aim of this study was to discover differential metabolites in the skin of Clapp's Favorite and its red mutation cultivar Red Clapp's Favorite. The metabolic components were detected using high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). Chromatographic experiments were performed on an HSS T3 column (100 mm×2.1 mm, 1.8 μm) by using a mobile phase consisting of 0.1% (v/v) formic acid in acetonitrile and water, and mass spectrometry was conducted in the positive and negative modes by electrospray ionization (ESI). Red Clapp's Favorite and Clapp's Favorite were collected from the pear germplasm resource nursery of Yantai Institute of Agricultural Sciences in Shandong. The data were analyzed by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) as well as cluster analysis and heat map. The first two principal components exhibited 62.3% and 8% of the total variance in the positive and negative ion modes, respectively. PCA can generally reflect metabolite differences between the two groups of samples, and there are significant differences in metabolites between the two cultivars. The results showed that PLS-DA clearly distinguishes the two groups of samples, which can be used to analyze the subsequent difference in components. The compounds were identified based on data retrieved from the PMDB databases according to the accurate mass number, secondary fragment, and isotope distribution. The results showed that the metabolite content in the skin of Red Clapp's Favorite and Clapp's Favorite were significant. There were 83 different metabolites (<0.05, variable importance in project (VIP)≥1), including phenols and amino acids, which are involved in flavonoid metabolism, amino acid metabolism, phenyl propanoid biosynthesis, and other metabolic pathways, including 5 polyphenols, 3 flavonoids, 1 amino acid and derivatives, 8 phenylpropanes, 2 anthocyanins, 5 proanthocyanidins, 6 flavanols, 14 flavonols, 2 isoflavones, 13 triterpenoids, 3 organic acids and derivatives, 1 vitamin, 3 organic acids and derivatives, 15 lipids, and 2 other compounds. The chlorogenic acid and crypto-chlorogenic acid in Red Clapp's Favorite are 2.40 and 3.46 times as much as those in Clapp's Favorite. The anthocyanins of cornulin 3-glucoside and cornulin 3-galactoside were 10.235 and 9.394 times, respectively. Phenolic epicatechin and catechin increased by 4.689 and 4.635, respectively. The content of phenylpropane 3, 4-dihydroxycinnamic acid in Red Clapp's Favorite increased by 3.13 times. Among the 83 differential metabolites, 23 metabolites were enriched in the pathway. To display the relationship between the samples and the differences in metabolites among the different samples intuitively, hierarchical clustering and heat map analysis were performed on the metabolite expression levels with significant differences in the enrichment pathways. The Kyoto Encyclopedia of Genes and Genomes database was used to further analyze the pathway enrichment of different metabolites. According to the results, there were 6 metabolic pathways (<0.05): flavonoid biosynthesis, flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, butanoate metabolism, phenylalanine metabolism, and tyrosine metabolism. Plant secondary metabolism shows a complex diversity. This study would screen out other pathways affecting the biosynthesis of flavonoids, which could provide reference for the further study of biosynthesis and biological function of flavonoids in red fruits. This study provides a useful reference for metabolomics of red pears, which could provide a theoretical reference for the quality analysis and biological function research of pears.
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http://dx.doi.org/10.3724/SP.J.1123.2021.05002DOI Listing
November 2021

Comparison of the optical quality after SMILE and FS-LASIK for high myopia by OQAS and iTrace analyzer: a one-year retrospective study.

BMC Ophthalmol 2021 Aug 2;21(1):292. Epub 2021 Aug 2.

Eye Center of Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: To compare the correction effect and optical quality after small-incision lenticule extraction (SMILE) and femtosecond laser assisted laser in situ keratomileusis (FS-LASIK) for high myopia.

Methods: 51 high myopia eyes after SMILE and 49 high myopia eyes after FS-LASIK were enrolled and divided into two groups retrospectively. The OQAS and iTrace analyzer were used for optical quality inspection. Between the two groups the spherical equivalent (SE), astigmatism, uncorrected distant visual acuity (UDVA), strehl ratio (SR), modulation transfer function cutoff frequency (MTF cutoff), objective scatter index (OSI) and wavefront aberrations were analyzed and compared before surgery and at 1, 6 and 12 months after surgery.

Results: After the operation: (1) SE and astigmatism declined and UDVA increased significantly in both groups, and UDVA was better after SMILE than FS-LASIK. (2) SR and MTF cutoff reduced and OSI increased significantly after SMILE and FS-LASIK. SR and MTF cutoff were significantly higher after SMILE than FS-LASIK. OSI was significantly lower after SMILE than FS-LASIK. (3) The total wavefront aberration, total low-order wavefront aberration, defocus and astigmatism aberration as well as trefoil aberration reduced significantly in both groups. The total high-order wavefront aberration increased significantly after FS-LASIK. The spherical and coma aberration increased significantly in both groups. The total high-order wavefront aberration and coma aberration at 1 month were higher after FS-LASIK than SMILE.

Conclusion: The optical quality descended after SMILE and FS-LASIK. SMILE was superior to FS-LASIK at the correction effect and optical quality for high myopia. The combination of OQAS and iTrace analyzer is a valuable complementary measurement in evaluating the optical quality after the refractive surgery.

Trial Registration: This is a retrospective study. This research was approved by the ethics committee of Xiangya Hospital and the IRB approval number is 201612074.
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http://dx.doi.org/10.1186/s12886-021-02048-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330115PMC
August 2021

Prevalence of comorbidity in Chinese patients with COVID-19: systematic review and meta-analysis of risk factors.

BMC Infect Dis 2021 Feb 22;21(1):200. Epub 2021 Feb 22.

Queen Mary School, Nanchang University, Nanchang, Jiangxi, China.

Background: Coronavirus disease 2019 (COVID-19) is an infectious disease characterized by cough, fever, and fatigue and 20% of cases will develop into severe conditions resulting from acute lung injury with the manifestation of the acute respiratory distress syndrome (ARDS) that accounts for more than 50% of mortality. Currently, it has been reported that some comorbidities are linked with an increased rate of severity and mortality among COVID-19 patients. To assess the role of comorbidity in COVID-19 progression, we performed a systematic review with a meta-analysis on the relationship of COVID-19 severity with 8 different underlying diseases.

Methods: PubMed, Web of Science, and CNKI were searched for articles investigating the prevalence of comorbidities in severe and non-severe COVID-19 patients. A total of 41 studies comprising 12,526 patients were included.

Results: Prevalence of some commodities was lower than that in general population such as hypertension (19% vs 23.2%), diabetes (9% vs 10.9%), chronic kidney disease (CKD) (2% vs 9.5%), chronic liver diseases (CLD) (3% vs 24.8%) and chronic obstructive pulmonary disease (COPD) (3% vs 8.6%), while some others including cancer (1% vs 0.6%), cardiovascular disease (6% vs 1.8%) and cerebrovascular disease (2% vs 0.9%) exhibited greater percentage in COVID-19. Cerebrovascular disease (OR = 3.70, 95%CI 2.51-5.45) was found to be the strongest risk factor in disease exacerbation, followed by CKD (OR = 3.60, 95%CI 2.18-5.94), COPD (OR = 3.14, 95% CI 2.35-4.19), cardiovascular disease (OR = 2.76, 95% CI 2.18-3.49), malignancy (OR = 2.63, 95% CI 1.75-3.95), diabetes (OR = 2.49, 95% CI 2.10-2.96) and hypertension (OR = 2.13, 95% CI 1.81-2.51). We found no correlation between CLD and increased disease severity (OR = 1.32, 95% CI 0.96-1.82).

Conclusion: The impact of all eight underlying diseases on COVID-19 deterioration seemed to be higher in patients outside Hubei. Based on different comorbidities, COVID-19 patients tend to be at risk of developing poor outcomes to a varying degree. Thus, tailored infection prevention and monitoring and treatment strategies targeting these high-risk subgroups might improve prognosis during the COVID-19 pandemic.
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http://dx.doi.org/10.1186/s12879-021-05915-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897883PMC
February 2021

Clinical outcomes after small-incision lenticule extraction versus femtosecond laser-assisted LASIK for high myopia: A meta-analysis.

PLoS One 2021 8;16(2):e0242059. Epub 2021 Feb 8.

Department of Ophthalmology, XiangYa Hospital, Central South University, Changsha, Hunan, China.

Aim: To compare postoperative clinical outcomes of high myopia after small-incision lenticule extraction (SMILE) and femtosecond laser-assisted laser in situ keratomileusis (FS-LASIK).

Methods: From March 2018 to July 2020, PubMed, MEDLINE, Embase, the Cochrane Library, and several Chinese databases were comprehensively searched. The studies meeting the criteria were selected and included; the data were extracted by 2 independent authors. The clinical outcome parameters were analyzed with RevMan 5.3.

Results: This meta-analysis included twelve studies involving 766 patients (1400 eyes: 748 receiving SMILE and 652 receiving FS-LASIK). Pooled results revealed no significant differences in the following outcomes: the logarithm of the mean angle of resolution (logMAR) of postoperative uncorrected distance visual acuity (weighted mean difference (WMD) = -0.01, 95% confidence interval (CI): -0.02 to 0.00, I2 = 0%, P = 0.07 at 1 mo; WMD = -0.00, 95% CI: -0.01 to 0.01, I2 = 0%, P = 0.83 at 3 mo; WMD = -0.00, 95% CI: -0.01 to 0.00, I2 = 32%, P = 0.33 in the long term), and the postoperative mean refractive spherical equivalent (WMD = -0.03, 95% CI: -0.09 to 0.03, I2 = 13%, P = 0.30). However, the SMILE group had significantly better postoperative corrected distance visual acuity (CDVA) than the FS-LASIK group (WMD = -0.04, 95% CI, -0.05 to -0.02, I2 = 0%, P<0.00001). In the long term, postoperative total higher-order aberration (WMD = -0.09, 95% CI: -0.10 to -0.07, I2 = 7%, P<0.00001) and postoperative spherical aberration (WMD = -0.15, 95% CI: -0.19 to -0.11, I2 = 29%, P<0.00001) were lower in the SMILE group than in the FS-LASIK group; a significant difference was also found in postoperative coma (WMD = -0.05, 95% CI: -0.06 to -0.03, I2 = 30%, P<0.00001).

Conclusion: For patients with high myopia, both SMILE and FS-LASIK are safe, efficacious and predictable. However, the SMILE group demonstrated advantages over the FS-LASIK group in terms of postoperative CDVA, while SMILE induced less aberration than FS-LASIK. It remains to be seen whether SMILE can provide better visual quality than FS-LASIK; further comparative studies focused on high myopia are necessary.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242059PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870077PMC
July 2021

Mannose synergizes with chemoradiotherapy to cure cancer via metabolically targeting HIF-1 in a novel triple-negative glioblastoma mouse model.

Clin Transl Med 2020 Nov;10(7):e226

Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Key Laboratory of Ministry of Education for Neurological Disorders, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1002/ctm2.226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648968PMC
November 2020

Pulsed terahertz spectroscopy combined with hybrid machine learning approaches for structural health monitoring of multilayer thermal barrier coatings.

Opt Express 2020 Nov;28(23):34875-34893

Structural health monitoring of multilayer thermal barrier coatings (TBCs) is very vital to ensure the structural integrity and service performance of the hot-section components of the aero-engine. In this paper, we theoretically and numerically demonstrated that the terahertz time domain spectrum and the terahertz reflectance spectrum could be adopted to estimate the structure parameters, based on the finite difference time domain (FDTD) algorithm, 64 samples which were imported with three kinds of 64 sets structure parameters had been calculated to obtain the time domain and terahertz reflectance signals. To mimic the actual test signals, the original FDTD simulation signals were processed by adding the Gaussian white noise and wavelet noise reduction. To reduce the data dimension and improve the calculation efficiency during modeling, the principal component analysis (PCA) algorithm was adopted to reduce the dimensions of time-domain data and reflectance data. Finally, these data after multiple signal processing and PCA feature extraction were used to train the extreme learning machine (ELM), combining the genetic algorithm (GA) could optimize the PCA-ELM model and further improve the prediction performance of the hybrid model. Our proposed novel and efficient terahertz nondestructive technology combined with the hybrid machine learning approaches provides great potential applications on the multilayer TBCs structural integrity evaluation.
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http://dx.doi.org/10.1364/OE.404042DOI Listing
November 2020

Clinical characteristics of imported and second-generation coronavirus disease 2019 (COVID-19) cases in Shaanxi outside Wuhan, China: a multicentre retrospective study.

Epidemiol Infect 2020 09 30;148:e238. Epub 2020 Sep 30.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.

The mortality of coronavirus disease 2019 (COVID-19) differs between countries and regions. This study aimed to clarify the clinical characteristics of imported and second-generation cases in Shaanxi. This study included 134 COVID-19 cases in Shaanxi outside Wuhan. Clinical data were compared between severe and non-severe cases. We further profiled the dynamic laboratory findings of some patients. In total, 34.3% of the 134 patients were severe cases, 11.2% had complications. As of 7 March 2020, 91.8% patients were discharged and one patient (0.7%) died. Age, lymphocyte count, C-reactive protein, erythrocyte sedimentation rate, direct bilirubin, lactate dehydrogenase and hydroxybutyrate dehydrogenase showed difference between severe and no-severe cases (all P < 0.05). Baseline lymphocyte count was higher in survived patients than in non-survivor case, and it increased as the condition improved, but declined sharply when death occurred. The interleukin-6 (IL-6) level displayed a downtrend in survivors, but rose very high in the death case. Pulmonary fibrosis was found on later chest computed tomography images in 51.5% of the pneumonia cases. Imported and second-generation cases outside Wuhan had a better prognosis than initial cases in Wuhan. Lymphocyte count and IL-6 level could be used for evaluating prognosis. Pulmonary fibrosis as the sequelae of COVID-19 should be taken into account.
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http://dx.doi.org/10.1017/S0950268820002332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7562779PMC
September 2020

Carbon [email protected] composites with improved mechanical and biological properties fabricated by a multi in situ synthesis process.

Biomed Microdevices 2020 09 8;22(4):64. Epub 2020 Sep 8.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, China.

A novel carbon [email protected] ([email protected]) composite with good mechanical and biological properties was fabricated successfully by a multi in situ synthesis process, which can be used to repair or replace the damaged bone tissues. The carbon nanotube (CNT)/hydroxyapatite (HA) composite powders were firstly synthesized by the in situ chemical vapor deposition method. After the acidification of CNTs, the collagen (Col) molecules were covalently grafted onto the surface of CNTs in situ by the formation of amide linkages, obtaining Col-encapsulated CNTs powders. And then, a HA layer was deposited in situ onto the Col-encapsulated CNTs to form HA- and Col-encapsulated CNTs, consequently the ideal [email protected] composite was fabricated by the powder metallurgy method, and its mechanical and biological properties were investigated. The results showed that, the multi in situ synthesis process ensured the homogeneous dispersion of CNTs in HA matrix, and via the intermediate layer of Col, the close chemical bonding between CNT reinforcements and HA matrix was obtained, thereby the flexural strength and fracture toughness of the in situ synthesized 3 wt.% [email protected] composite were increased by approximately 74.2% and 274.6% compared with those of pure HA bulk, and better cell adhesion, spreading and proliferation were also observed on the in situ synthesized [email protected] composites. Therefore, the obtained composites in this work have great potential to be applied as implant material in clinic.
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http://dx.doi.org/10.1007/s10544-020-00520-5DOI Listing
September 2020

Mutations at the dimer interface and surface residues of Nm23-H1 metastasis suppressor affect its expression and function.

Mol Cell Biochem 2020 Nov 23;474(1-2):95-112. Epub 2020 Jul 23.

Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China.

The Nm23 metastasis suppressor family is involved in a variety of physiological and pathological processes including cell proliferation, differentiation, tumorigenesis, and metastasis. Given that Nm23 proteins may function as hexamers composed of different members of the family, especially Nm23-H1 and H2 isoforms, it is pertinent to assess the importance of interface and surface residues in defining the functional characteristics of Nm23 proteins. Using molecular modeling to identify clusters of residues that may affect dimer formation and isoform specificity, mutants of Nm23-H1 were constructed and assayed for their ability to modulate cell migration. Mutations of dimer interface residues Gly and Lys affected the expression level of Nm23-H1, without altering the transcript level. The reduced protein expression was not due to increased protein degradation or altered subcellular distribution. Substitution of the surface residues of Nm23-H1 with Nm23-H2-specific Ser and/or Lys affected the electrophoretic mobility of the protein. Moreover, in cell migration assays, several mutants with altered surface residues exhibited impaired ability to suppress the mobility of MDA-MB-231 cells. Collectively, the study suggests that disrupting the dimer interface may affect the expression of Nm23-H1, while the residues at α-helix and β-sheet on the surface of Nm23-H1 may contribute to its metastasis suppressive function.
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http://dx.doi.org/10.1007/s11010-020-03836-1DOI Listing
November 2020

Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II, and Pol III.

Genome Biol 2020 07 2;21(1):158. Epub 2020 Jul 2.

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.

Background: The relationship between transcription and the 3D chromatin structure is debated. Multiple studies have shown that transcription affects global Cohesin binding and 3D genome structures. However, several other studies have indicated that inhibited transcription does not alter chromatin conformations.

Results: We provide the most comprehensive evidence to date to demonstrate that transcription plays a relatively modest role in organizing the local, small-scale chromatin structures in mammalian cells. We show degraded Pol I, Pol II, and Pol III proteins in mESCs cause few or no changes in large-scale 3D chromatin structures, selected RNA polymerases with a high abundance of binding sites or active promoter-associated interactions appear to be relatively more affected after the degradation, transcription inhibition alters local, small loop domains, as indicated by high-resolution chromatin interaction maps, and loops with bound Pol II but without Cohesin or CTCF are identified and found to be largely unchanged after transcription inhibition. Interestingly, Pol II depletion for a longer time significantly affects the chromatin accessibility and Cohesin occupancy, suggesting that RNA polymerases are capable of affecting the 3D genome indirectly. These direct and indirect effects explain the previous inconsistent findings on the influence of transcription inhibition on the 3D genome.

Conclusions: We conclude that Pol I, Pol II, and Pol III loss alters local, small-scale chromatin interactions in mammalian cells, suggesting that the 3D chromatin structures are pre-established and relatively stable.
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http://dx.doi.org/10.1186/s13059-020-02067-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331254PMC
July 2020

MIMU/Odometer Fusion with State Constraints for Vehicle Positioning during BeiDou Signal Outage: Testing and Results.

Sensors (Basel) 2020 Apr 17;20(8). Epub 2020 Apr 17.

Department of Photogrammetry and Remote Sensing, Finnish Geospatial Research Institute, Masala, FI-0245 Espoo, Finland.

With the rapid development of autonomous vehicles, the demand for reliable positioning results is urgent. Currently, the ground vehicles heavily depend on the Global Navigation Satellite System (GNSS) and the Inertial Navigation System (INS) providing reliable and continuous navigation solutions. In dense urban areas, especially narrow streets with tall buildings, the GNSS signals are possibly blocked by the surrounding tall buildings, and under this condition, the geometry distribution of the in-view satellites is very poor, and the None-Line-Of-Sight (NLOS) and Multipath (MP) heavily affects the positioning accuracy. Further, the INS positioning errors will quickly diverge over time without the GNSS correction. Aiming at improving the position accuracy under signal challenging environment, in this paper, we developed an MIMU(Micro Inertial Measurement Unit)/Odometer integration system with vehicle state constraints (MO-C) for improving the vehicle positioning accuracy without GNSS. MIMU/Odometer integration model and the constrained measurements are given in detail. Several field tests were carried out for evaluating and assessing the MO-C system. The experiments were divided into two parts, firstly, field testing with data post-processing and real-time processing was carried out for fully assessing the performance of the MO-C system. Secondly, the MO-C was implemented in the BeiDou Satellite Navigation System (BDS)/integrated navigation system (INS) for evaluating the MO-C performance during the BDS signal outage. The MIMU standalone positioning accuracy was compared with that from the MIMU/Odometer integration (MO), MO-C and MIMU with constraints (M-C) for assessing the Odometer, and the influence of the constraint on the positioning errors reduction. The results showed that the latitude and longitude errors could be suppressed with Odometer assisting, and the height errors were suppressed while the state constraints were included.
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http://dx.doi.org/10.3390/s20082302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219075PMC
April 2020

Identification of key genes related to seedlessness by genome-wide detection of structural variation and transcriptome analysis in 'Shijiwuhe' pear.

Gene 2020 May 17;738:144480. Epub 2020 Feb 17.

Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

Seedless fruits are highly marketable because they are easier to eat than fruits with seeds. 'Shijiwuhe' is a seedless pear cultivar that is a mutant derived from an F1 hybridization population ('Bartlett' x 'Yali'). Little is known about the key genes controlling seedless pear fruit. In this study, field experiments revealed that seedless 'Shijiwuhe' pear was not due to parthenocarpy, and that it was self-incompatible. Single nucleotide polymorphisms (SNPs), small insertions and deletions (InDels) and structural variations (SVs) were characterized using DNA sequencing data between 'Shijiwuhe' and parental cultivars. A total of 1498 genes were found to be affected by SV and over 50% of SVs were located in promoter regions. Transcriptome analysis was conducted at three time points (4, 8, and 12 days after cross-pollination) during early fruit development of 'Shijiwuhe', 'Bartlett', and 'Yali'. In total, 1438 differentially expressed genes (DEGs) were found between 'Shijiwuhe' and parental cultivars 'Bartlett' and 'Yali'. We found 1193 SVs that caused differential expression of genes at 4 DACP. Among them, over 100 genes were in pathways related to seed nutrition and energy storage and 41 candidate genes encoded several important transcription factors, such as MYB, WRKY, NAC, and bHLH, which might play important roles in seed development. The qRT-PCR results also confirmed that the candidate genes with SVs showed differential expression between 'Shijiwuhe' pear and 'Bartlett' or 'Yali'. This study, which combined field experiments, SV detection, and transcriptome analysis might provide an effective way to predict the candidate genes regulating the seedless trait and important gene resources for genetic improvement of pear.
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http://dx.doi.org/10.1016/j.gene.2020.144480DOI Listing
May 2020

Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits.

Plant Biotechnol J 2020 05 19;18(5):1223-1240. Epub 2019 Nov 19.

Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang, China.

Flavonoid accumulation in most fruits is enhanced by ethylene and jasmonate. However, little is known about the hormone functions related to red pear fruit coloration or their combined effects and potential underlying mechanisms. Various treatments were used to investigate the flavonoid metabolite profile and pear transcriptome to verify the effects of ethylene and jasmonate on flavonoid biosynthesis in red pear fruits as well as the mechanism behind this. Ethylene inhibits anthocyanin biosynthesis in red Chinese pear fruits, whereas jasmonate increases anthocyanin and flavone/isoflavone biosyntheses. The branching of the jasmonate-induced flavonoid biosynthesis pathway is determined by ethylene. Co-expression network and Mfuzz analyses revealed 4,368 candidate transcripts. Additionally, ethylene suppresses PpMYB10 and PpMYB114 expression via TF repressors, ultimately decreasing anthocyanin biosynthesis. Jasmonate induces anthocyanin accumulation through transcriptional or post-translational regulation of TFs-like MYB and bHLH in the absence of ethylene. However, jasmonate induces ethylene biosynthesis and the associated signalling pathway in pear, thereby decreasing anthocyanin production, increasing the availability of the precursors for flavone/isoflavone biosynthesis and enhancing deep yellow fruit coloration. We herein present new phenotypes and fruit coloration regulatory patterns controlled by jasmonate and ethylene, and confirm that the regulation of fruit coloration is complex.
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http://dx.doi.org/10.1111/pbi.13287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152598PMC
May 2020

Dual Roles of the AMP-Activated Protein Kinase Pathway in Angiogenesis.

Cells 2019 07 19;8(7). Epub 2019 Jul 19.

Jiangxi Provincial Key Laboratory of Tumor Pathogens and Molecular Pathology, Department of Pathophysiology, School of Basic Medical Sciences, Nanchang University Jiangxi Medical College, Nanchang, Jiangxi, Post Code 330006, China.

Angiogenesis plays important roles in development, stress response, wound healing, tumorigenesis and cancer progression, diabetic retinopathy, and age-related macular degeneration. It is a complex event engaging many signaling pathways including vascular endothelial growth factor (VEGF), Notch, transforming growth factor-beta/bone morphogenetic proteins (TGF-β/BMPs), and other cytokines and growth factors. Almost all of them eventually funnel to two crucial molecules, VEGF and hypoxia-inducing factor-1 alpha (HIF-1α) whose expressions could change under both physiological and pathological conditions. Hypoxic conditions stabilize HIF-1α, while it is upregulated by many oncogenic factors under normaxia. HIF-1α is a critical transcription activator for VEGF. Recent studies have shown that intracellular metabolic state participates in regulation of sprouting angiogenesis, which may involve AMP-activated protein kinase (AMPK). Indeed, AMPK has been shown to exert both positive and negative effects on angiogenesis. On the one hand, activation of AMPK mediates stress responses to facilitate autophagy which stabilizes HIF-1α, leading to increased expression of VEGF. On the other hand, AMPK could attenuate angiogenesis induced by tumor-promoting and pro-metastatic factors, such as the phosphoinositide 3-kinase /protein kinase B (Akt)/mammalian target of rapamycin (PI3K/Akt/mTOR), hepatic growth factor (HGF), and TGF-β/BMP signaling pathways. Thus, this review will summarize research progresses on these two opposite effects and discuss the mechanisms behind the discrepant findings.
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http://dx.doi.org/10.3390/cells8070752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678403PMC
July 2019

Pretreatment of Grape Seed Proanthocyanidin Extract Exerts Neuroprotective Effect in Murine Model of Neonatal Hypoxic-ischemic Brain Injury by Its Antiapoptotic Property.

Cell Mol Neurobiol 2019 Oct 30;39(7):953-961. Epub 2019 May 30.

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, People's Republic of China.

Grape seed proanthocyanidin extract (GSPE), an active component extracted from the grape, has been reported to demonstrate antioxidant, anti-inflammatory, anticancer, and antiapoptosis effects. However, little is known about the role of GSPE on neonatal hypoxic-ischemic (HI) brain injury. The aim of this study was to evaluate the neuroprotective effect of GSPE pretreatment on neonatal HI brain injury in mice. A modified Rice-Vannucci method was performed to induce neonatal HI brain injury in the 7-day-old mouse pups pretreated with GSPE or vehicle. The infarct volumes were determined by TTC staining. TUNEL staining was used to detect cells apoptosis, and the expressions of apoptosis-related proteins: bax, bcl2, and cleaved caspase-3 were assayed by Western blot. Behavioral tests were also conducted to assess the functional recovery after injury. We showed that the brain damage and neurobehavioral outcomes improvement was observed in GSPE pretreated group. GSPE was proved to suppress apoptosis through inhibition of bax and cleaved caspase-3 expression. It demonstrates that GSPE could alleviate brain damage maybe through its antiapoptotic activity in a neonatal HI brain injury model, and GSPE has the potential to be a new drug for effective prevention of this disorder.
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http://dx.doi.org/10.1007/s10571-019-00691-7DOI Listing
October 2019

Sorafenib-Loaded Ligand-Functionalized Polymer-Lipid Hybrid Nanoparticles for Enhanced Therapeutic Effect Against Liver Cancer.

J Nanosci Nanotechnol 2019 11;19(11):6866-6871

The Outpatient Department of the Ministry of Support, The 92493 Unit of the Chinese People's Liberation Army, Huludao City, Liaoning Province, 125000, China.

The main aim of present study was to enhance the anticancer effect of sorafenib (SRF) in the liver cancer cells. The SRF-loaded folate receptor-targeted polymer lipid hybrid nanoparticle was formulated to enhance the therapeutic efficacy in the treatment of liver cancers. The SRF-loaded folic acid (FA)-conjugated lipid-coated chitosan/chondroitin sulfate (CT/CS) nanoparticle (FCCD/SRF) showed a remarkable internalization of cancer cells compared to non-targeted CCD/SRF. The higher internalization of nanoparticle was mainly attributed to the specific interaction to the folate receptors overexpressed in the liver cancer cells. FCCD/SRF exhibited a remarkable cell killing effect throughout all tested concentrations. Consistent with the cytotoxic effect, IC50 value of FCCD/SRF was 0.78 g/ml compared to 3.92 g/ml for CCD/SRF indicating the potential of targeting strategy to the cancer cells. FCCD/SRF showed a remarkable apoptosis of cancer cells with distorted nucleus and apoptotic body formation. Overall, results showed that folate-conjugated polymer-lipid hybrid nanoparticle possess promising potential for anticancer drug delivery in the treatment of liver cancers.
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http://dx.doi.org/10.1166/jnn.2019.16936DOI Listing
November 2019

Interleukin-17: The Role for Pathological Angiogenesis in Ocular Neovascular Diseases.

Tohoku J Exp Med 2019 02;247(2):87-98

Department of Ophthalmology, The Second Xiangya Hospital, Central South University.

Ocular neovascular diseases are featured by abnormal angiogenesis in the eye, and they seriously threaten the human visual health. These diseases include proliferative diabetic retinopathy (PDR), age-related macular degeneration (AMD), retinopathy of prematurity (ROP), and retinal vein occlusion (RVO). In fact, ocular neovascular diseases represent the leading causes of vision impairment and blindness worldwide. Ocular neovascularization, the process of pathological vessel formation in eye, underlies ocular neovascular diseases. Cytokines have important regulatory roles in neovascularization through immunological networks. Interleukin (IL)-17, the signature cytokine produced by T helper 17 (Th17) cells, has proven to be involved in ocular neovascularization. However, roles of IL-17 in ocular neovascular diseases still remain controversial. This review provides an overview of the functional roles of IL-17 in ocular neovascular diseases from basic research to clinical evidence by focusing on PDR, AMD, ROP, and RVO. The possible roles of IL-17 in neovascularization are achieved through a regulatory network of cytoskeleton remodeling, vascular endothelial growth factor (VEGF), VEGF-related cytokines, and complement components. Current applications as well as potential therapies targeting IL-17 with genome editing systems are also outlined and discussed. Targeting IL-17 might be a promising therapeutic strategy against ocular neovascular diseases.
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http://dx.doi.org/10.1620/tjem.247.87DOI Listing
February 2019

Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier.

Mater Sci Eng C Mater Biol Appl 2019 Apr 26;97:222-229. Epub 2018 Nov 26.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China.

A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX). The synthesized CNTs, Fe, and HA self-assembled into a composite structure in situ during the synthesis. After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs. Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA. The results showed that the average drug-loading rate of DOX was 130 wt%. Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h. In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%. In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.
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http://dx.doi.org/10.1016/j.msec.2018.11.042DOI Listing
April 2019

Do microvascular changes occur preceding neural impairment in early-stage diabetic retinopathy? Evidence based on the optic nerve head using optical coherence tomography angiography.

Acta Diabetol 2019 May 17;56(5):531-539. Epub 2019 Jan 17.

Department of Ophthalmology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yanjiang West Road, Guangzhou, 510000, People's Republic of China.

Aims: To evaluate the microvascular and neural differences of the optic nerve head (ONH) between type 2 diabetes mellitus (T2DM) subjects and controls.

Methods: This was a cross-sectional observational study. One hundred and eight eyes of 108 T2DM subjects with or without diabetic retinopathy (DR) (54 preclinical DR and 54 mild-to-moderate DR) were included. Fifty-two eyes of 52 healthy subjects were included as controls. The 4.5-mm Angio Disc scan mode and the ganglion cell complex scan mode were performed with all participants using AngioVue software 2.0 of the optical coherence tomography angiography (OCTA) device.

Results: Regarding ONH radial peripapillary capillary (RPC) density, the peripapillary region was mainly significantly reduced in the No-DR (NDR) group. Moreover, the RPC density of the peripapillary region and the inside optic disc area were significantly reduced in the non-proliferative DR (NPDR) group. When compared to the controls, significantly reduced peripapillary capillary density in six sections was observed in the NPDR group. However, reduced density was observed in only two sections in the NDR group. The NPDR group had significantly increased focal loss volume (FLV) and reduced peripapillary RNFL thickness in the inferior nasal section compared to those in the controls, but similar changes were not observed in the NDR group. A regression model identified RPCs inside the optic disc as a significant parameter in early-stage DR detection. In the NPDR group, BCVA showed a significantly negative correlation with RPCs inside the optic disc and a significantly positive correlation with FLV.

Conclusions: OCTA findings of the ONH area may provide evidence that microvascular changes occur preceding neural impairment in early-stage DR. However, further researches are still needed to support the statement. Reduced ONH perfusion inside the optic disc may be one of the crucial biomarkers in early-stage DR detection and is a possible sensitive visual acuity predictor in early-stage DR subjects. With the ONH mode, OCTA may be a more promising tool in DR screening.
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http://dx.doi.org/10.1007/s00592-019-01288-8DOI Listing
May 2019

Targeting JNK pathway promotes human hematopoietic stem cell expansion.

Cell Discov 2019 8;5. Epub 2019 Jan 8.

1State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, Guangdong, 518055 China.

The limited number of human hematopoietic stem cells (HSCs) has restrained their widespread clinical application. Despite great efforts in recent years, the in vitro expansion of HSCs remains a challenge due to incomplete understanding of the signaling networks underlying HSC self-renewal. Here, we show that culturing human cord blood (CB) CD34 cells with JNK-IN-8, an inhibitor of the JNK signaling pathway, can enhance the self-renewal of HSCs with a 3.88-fold increase in cell number. These cultured CD34 cells repopulated recipient mice for 21 weeks and can form secondary engraftment that lasted for more than 21 weeks. Knockdown of , a major downstream target in the JNK pathway, promoted the expansion of hematopoietic stem and progenitor cells (HSPCs). Our findings demonstrate a critical role of the JNK pathway in regulating HSC expansion, provide new insights into HSC self-renewal mechanism, and may lead to improved clinical application of HSCs.
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http://dx.doi.org/10.1038/s41421-018-0072-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323118PMC
January 2019

Network analysis reveals the co-expression of sugar and aroma genes in the Chinese white pear (Pyrus bretschneideri).

Gene 2018 Nov 9;677:370-377. Epub 2018 Aug 9.

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

The quality of pear and other fruit affects their economic value by determining their attractiveness to the consumer. For this reason the two most important quality traits, namely the sugar content and the aroma, have been the focus of much research. This research has been conducted both on the biochemical characteristics of sugar and aroma of fruits and on the activity of enzymes related to their metabolism. In this study, by analyzing the transcriptome of pear fruit, we built the first co-expression network of the genes related to sugar metabolism and those related to aroma, to reveal their connections. Genes that encode beta-glucosidases and sorbitol dehydrogenases are shown to be the hub genes within this network. This study has demonstrated the power of such network analysis for the first time in a non-model organism. Our research can lead to new discoveries that go beyond the previous focus on individual genes.
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http://dx.doi.org/10.1016/j.gene.2018.08.042DOI Listing
November 2018

The Comparison of Regional RNFL and Fundus Vasculature by OCTA in Chinese Myopia Population.

J Ophthalmol 2018 31;2018:3490962. Epub 2018 Jan 31.

Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Purpose: To determine the correlations between peripapillary vessel density, retinal nerve fibre layer (RNFL) thickness, and myopic indices at retina quadrants with optical coherence tomography angiography (OCTA) in Chinese.

Methods: Fifty-six subjects with a mean spherical equivalent (MSE) of -3.63 ± 0.29 D were included. Peripapillary RNFL thickness and retinal vessel density in four sectors (superior, nasal, inferior, and temporal quadrants) were determined by OCTA, and correlations of the main outcomes were analyzed.

Results: Negative correlations were found between the peripapillary RNFL thickness and axial length (AL) at superior ( = -0.335, = 0.001) and inferior ( = -0.551, < 0.001) quadrants. There was a significant positive correlation with spherical equivalent (SE) at the corresponding quadrants as well as at the nasal quadrant ( = 0.339, = 0.001; = 0.379, < 0.001; and = 0.209, = 0.039, resp.). Peripapillary retinal vessel density was also negatively correlated with AL at the nasal quadrant ( = -0.392, < 0.001), and only at the nasal quadrant, there was a positive correlation between the peripapillary vessel density and SE ( = 0.319, = 0.001).

Conclusions: The degree of myopia and elongation of AL were negatively correlated with peripapillary RNFL thickness at superior and inferior quadrants and with peripapillary retinal vessel density at the nasal quadrant.
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http://dx.doi.org/10.1155/2018/3490962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830980PMC
January 2018

RGS19 upregulates Nm23-H1/2 metastasis suppressors by transcriptional activation via the cAMP/PKA/CREB pathway.

Oncotarget 2017 Sep 22;8(41):69945-69960. Epub 2017 Jul 22.

Division of Life Sciences and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China.

The Nm23 metastasis suppressor family is involved in physiological and pathological processes including tumorigenesis and metastasis. Although the inverse correlation of Nm23 level with tumor metastasis potential has been widely observed, the mechanisms that regulate the expression of Nm23 remain poorly understood. Our previous studies have revealed that Nm23-H1/2 isoforms are upregulated by RGS19, a regulator of G protein signaling (RGS) protein which accelerates the termination of G signals. Here, we examined the ability of RGS19 to stimulate transcriptional regulation of Nm23 by screening a panel of luciferase reporter genes. Transient and stable overexpression of RGS19 upregulated the Nm23-H1/2 protein levels and activated several transcription factors including CREB, AP-1 and SRE in HEK293 cells. Interestingly, agents that increase the intracellular cAMP level and the phosphorylation of CREB (e.g., adrenergic receptor agonist, forskolin, and cAMP analogues) upregulated the expression of Nm23-H1/2 in HEK293 cells and several cancer cell lines including A549, HeLa, MDA-MB-231, and MDA-MB-435s cells. Conversely, inhibition of protein kinase A (PKA) by H-89 suppressed the phosphorylation of CREB and reduced the expression of Nm23-H1/2. Furthermore, activation of PKA attenuated cancer cell migration in wound healing and transwell assays. Collectively, these results revealed a PKA-dependent mechanism for controlling Nm23-H1/2 expression.
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http://dx.doi.org/10.18632/oncotarget.19509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5642529PMC
September 2017

Serum 25-hydroxyvitamin D status in chinese children with vitiligo: a case-control study.

Clin Pediatr (Phila) 2018 06 9;57(7):802-805. Epub 2017 Oct 9.

1 Department of Laboratory Medicine, Children's Hospital of Shanxi Province, Tai'yuan, China.

Background: Vitamin D can play a vital role in autoimmune diseases. Epidemiologic evidence demonstrates vitamin D deficiency excited in adult patients with vitiligo.

Objectives: To investigate 25-hydroxyvitamin D (25(OH)D) levels in children with vitiligo and explore possible relevant factors.

Methods: A total of 114 patients and 100 controls were included in our case-control study. We analyzed the required data collected by the questionnaire and examination to reveal the correlation with 25(OH)D levels.

Results: The mean serum 25(OH)D levels in patients and controls were 43.62 ± 19.23 and 67.87 ± 19.45 nmol/L, respectively. The rate of deficient in patients and controls are 14.9% and 2%, respectively. There was significant difference between the 2 groups. Significant differences also existed on different ages comparing serum vitamin D levels of patients with controls ( P < .001). Age was an independent factor affecting 25(OH)D level ( P = .032). Two (1.8%) and 4 (3.5%) of children with vitiligo have comorbid autoimmune diseases and family history, respectively. There was no correlation between sex, duration of disease, type of vitiligo, skin lesion location, stage, family history, and comorbid autoimmune diseases with 25(OH)D levels.

Conclusion: Serum vitamin D level was associated with onset vitiligo children. More sunshine and vitamin D fortified foods are necessary among children with age. The rates of children vitiligo with family history has decreased in China.
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http://dx.doi.org/10.1177/0009922817734362DOI Listing
June 2018

Comparative Transcriptome Analysis Identifies Putative Genes Involved in the Biosynthesis of Xanthanolides in Xanthium strumarium L.

Front Plant Sci 2016 30;7:1317. Epub 2016 Aug 30.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences Wuhan, China.

Xanthium strumarium L. is a traditional Chinese herb belonging to the Asteraceae family. The major bioactive components of this plant are sesquiterpene lactones (STLs), which include the xanthanolides. To date, the biogenesis of xanthanolides, especially their downstream pathway, remains largely unknown. In X. strumarium, xanthanolides primarily accumulate in its glandular trichomes. To identify putative gene candidates involved in the biosynthesis of xanthanolides, three X. strumarium transcriptomes, which were derived from the young leaves of two different cultivars and the purified glandular trichomes from one of the cultivars, were constructed in this study. In total, 157 million clean reads were generated and assembled into 91,861 unigenes, of which 59,858 unigenes were successfully annotated. All the genes coding for known enzymes in the upstream pathway to the biosynthesis of xanthanolides were present in the X. strumarium transcriptomes. From a comparative analysis of the X. strumarium transcriptomes, this study identified a number of gene candidates that are putatively involved in the downstream pathway to the synthesis of xanthanolides, such as four unigenes encoding CYP71 P450s, 50 unigenes for dehydrogenases, and 27 genes for acetyltransferases. The possible functions of these four CYP71 candidates are extensively discussed. In addition, 116 transcription factors that are highly expressed in X. strumarium glandular trichomes were also identified. Their possible regulatory roles in the biosynthesis of STLs are discussed. The global transcriptomic data for X. strumarium should provide a valuable resource for further research into the biosynthesis of xanthanolides.
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http://dx.doi.org/10.3389/fpls.2016.01317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003840PMC
September 2016

Successful treatment of ulcerated hemangiomas with a dual-wavelength 595- and 1064-nm laser system.

J Dermatolog Treat 2016 Nov 13;27(6):562-567. Epub 2016 Apr 13.

a Department of Dermatology , the First Affiliated Hospital of Jinan University , Guangzhou , People's Republic of China.

Background: Pulsed dye laser (PDL) treatment has been shown to be effective for the management of ulcerated areas of hemangiomas. However, targeting deeper dermal lesions requires a laser system that penetrates more deeply than a PDL. Thus, we applied a dual-wavelength 595- and 1064-nm laser system to the treatment of ulcerated hemangiomas.

Materials And Methods: Twenty-two infants with ulcerated hemangiomas (mean are, 3.8 cm) were reviewed. Ulcers were treated by debridement and the surface received 595-nm PDL treatment at 4-5 J/cm with a pulse width of 0.5 ms. Then, the remaining hemangioma excluding the ulcerated portion was treated with dual-wavelength laser. Laser treatment was repeated at two-week intervals until cutaneous healing was observed.

Results: Ulcerative lesions in 20 of the 22 patients (90.9%) healed entirely within 2 weeks following the first laser treatment. Two patients also required oral steroids, and one patient underwent two laser treatments. The follow-up period ranged from 6 to 24 months, and no evidence of recurrent ulceration or hemangioma regrowth was observed.

Conclusions: Our findings confirm that PDL treatment leads to a rapid decrease in pain and initiation of the healing process and indicate that dual-wavelength laser treatment offers rapid healing with few adverse effects.
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http://dx.doi.org/10.3109/09546634.2016.1164287DOI Listing
November 2016

Identification and Functional Characterization of Sesquiterpene Synthases from Xanthium strumarium.

Plant Cell Physiol 2016 Mar 8;57(3):630-41. Epub 2016 Feb 8.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Science, Wuhan 430074, China

Xanthium strumarium synthesizes various pharmacologically active sesquiterpenes. The molecular characterization of sesquiterpene biosynthesis in X. strumarium has not been reported so far. In this study, the cDNAs coding for three sesquiterpene synthases (designated as XsTPS1, XsTPS2 and XsTPS3) were isolated using the X. strumarium transcriptome that we recently constructed. XsTPS1, XsTPS2 and XsTPS3 were revealed to have primary activities forming germacrene D, guaia-4,6-diene and germacrene A, respectively, by either ectopic expression in yeast cells or purified recombinant protein-based in vitro assays. Quantitative real-time PCRs and metabolite analysis for the different plant parts showed that the transcript abundance of XsTPS1-XsTPS3 is consistent with the accumulation pattern of their enzymatic products, supporting their biochemical functions in vivo. In particular, we discovered that none of the XsTPS2 product, guaia-4,6-diene, can be detected in one of the X. strumarium cultivars used in this study (it was named the Hubei-cultivar), in which a natural deletion of two A bases in the XsTPS2 cDNA disrupts its activity, which further confirmed the proposed biochemical role of XsTPS2 in X. strumarium in vivo.
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http://dx.doi.org/10.1093/pcp/pcw019DOI Listing
March 2016

Differential microRNA Analysis of Glandular Trichomes and Young Leaves in Xanthium strumarium L. Reveals Their Putative Roles in Regulating Terpenoid Biosynthesis.

PLoS One 2015 25;10(9):e0139002. Epub 2015 Sep 25.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China, 430074.

The medicinal plant Xanthium strumarium L. (X. strumarium) is covered with glandular trichomes, which are the sites for synthesizing pharmacologically active terpenoids such as xanthatin. MicroRNAs (miRNAs) are a class of 21-24 nucleotide (nt) non-coding RNAs, most of which are identified as regulators of plant growth development. Identification of miRNAs involved in the biosynthesis of plant secondary metabolites remains limited. In this study, high-throughput Illumina sequencing, combined with target gene prediction, was performed to discover novel and conserved miRNAs with potential roles in regulating terpenoid biosynthesis in X. strumarium glandular trichomes. Two small RNA libraries from leaves and glandular trichomes of X. strumarium were established. In total, 1,185 conserved miRNAs and 37 novel miRNAs were identified, with 494 conserved miRNAs and 18 novel miRNAs being differentially expressed between the two tissue sources. Based on the X. strumarium transcriptome data that we recently constructed, 3,307 annotated mRNA transcripts were identified as putative targets of the differentially expressed miRNAs. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis suggested that some of the differentially expressed miRNAs, including miR6435, miR5021 and miR1134, might be involved in terpenoid biosynthesis in the X. strumarium glandular trichomes. This study provides the first comprehensive analysis of miRNAs in X. strumarium, which forms the basis for further understanding of miRNA-based regulation on terpenoid biosynthesis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139002PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583480PMC
May 2016

Trip6 promotes dendritic morphogenesis through dephosphorylated GRIP1-dependent myosin VI and F-actin organization.

J Neurosci 2015 Feb;35(6):2559-71

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and

Thyroid receptor-interacting protein 6 (Trip6), a multifunctional protein belonging to the zyxin family of LIM proteins, is involved in various physiological and pathological processes, including cell migration and tumorigenesis. However, the role of Trip6 in neurons remains unknown. Here, we show that Trip6 is expressed in mouse hippocampal neurons and promotes dendritic morphogenesis. Through interaction with the glutamate receptor-interacting protein 1 (GRIP1) and myosin VI, Trip6 is crucial for the total dendritic length and the number of primary dendrites in cultured hippocampal neurons. Trip6 depletion reduces F-actin content and impairs dendritic morphology, and this phenocopies GRIP1 or myosin VI knockdown. Furthermore, phosphorylation of GRIP1(956T) by AKT1 inhibits the interaction between GRIP1 and myosin VI, but facilitates GRIP1 binding to 14-3-3 protein, which is required for regulating F-actin organization and dendritic morphogenesis. Thus, the Trip6-GRIP1-myosin VI interaction and its regulation on F-actin network play a significant role in dendritic morphogenesis.
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http://dx.doi.org/10.1523/JNEUROSCI.2125-14.2015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605620PMC
February 2015
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