Publications by authors named "Yuanfan Yang"

12 Publications

  • Page 1 of 1

The F-box protein MIO1/SLB1 regulates organ size and leaf movement in Medicago truncatula.

J Exp Bot 2021 Jan 28. Epub 2021 Jan 28.

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China.

The size of leaf and seed organs, determined by the interplay of cell proliferation and expansion, is closely related to the final yield and quality of forage and crops. Yet the cellular and molecular mechanisms underlying organ size modulation remain poorly understood, especially in legumes. Here, MINI ORGAN1 (MIO1) was identified as a key regulator of organ size, which encodes an F-box protein (SLB1) was recently reported to control lateral branching in M. truncatula. We show that loss of function of MIO1/SLB1 severely reduced organ size. Conversely, plants with overexpression of MIO1/SLB1 plants had enlarged organs. Cellular analysis revealed that MIO1/SLB1 controlled organ size mainly by modulating primary cell proliferation during the early stages of leaf development. Biochemistry analysis revealed MIO1/SLB1 could form part of an SKP1/Cullin/F-box (SCF) E3 ubiquitin ligase complex, to target BIG SEEDS1 (BS1), a repressor of primary cell division for degradation. Interestingly, we found that MIO1/SLB1 also played a key role in pulvinus development and leaf movement by modulating cell proliferation of the pulvinus as leaves developed. Our study not only demonstrates a conserved role of MIO1/SLB1 for organ size control in legumes, but also sheds light on the novel function of MIO1/SLB1 in leaf movement.
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http://dx.doi.org/10.1093/jxb/erab033DOI Listing
January 2021

Tyrosinase inhibition by -coumaric acid ethyl ester identified from camellia pollen.

Food Sci Nutr 2021 Jan 11;9(1):389-400. Epub 2020 Dec 11.

College of Food and Biological Engineering Jimei University Xiamen China.

A tyrosinase inhibitor was separated from camellia pollen with the aid of solvent fraction, macroporous adsorptive resin chromatography, and high-speed countercurrent chromatography. The inhibitor was identified to be -coumaric acid ethyl ester (-CAEE) by nuclear magnetic resonance and mass spectrum. Its inhibitory activity (IC = 4.89 μg/ml) was about 10-fold stronger than arbutin (IC = 51.54 μg/ml). The -CAEE inhibited tyrosinase in a noncompetitive model with the and of 1.83 μg/ml and 0.52 mM, respectively. Fluorescence spectroscopy analysis showed the -CAEE quenched an intrinsic fluorescence tyrosinase. UV-Vis spectroscopy analysis showed the -CAEE did not interact with copper ions of the enzyme. Docking simulation implied the CAEE induced a conformational change in the catalytic region and thus changed binding forces of L-tyrosine. Our findings suggest that -CAEE plays an important role in inhibiting tyrosinase and provides a reference for developing pharmaceutical, cosmetic, and fruit preservation products using pollen.
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http://dx.doi.org/10.1002/fsn3.2004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802545PMC
January 2021

The WOX family transcriptional regulator SlLAM1 controls compound leaf and floral organ development in Solanum lycopersicum.

J Exp Bot 2021 Feb;72(5):1822-1835

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China.

Plant-specific WOX family transcription factors play important roles ranging from embryogenesis to lateral organ development. The WOX1 transcription factors, which belong to the modern clade of the WOX family, are known to regulate outgrowth of the leaf blade specifically in the mediolateral axis; however, the role of WOX1 in compound leaf development remains unknown. Phylogenetic analysis of the whole WOX family in tomato (Solanum lycopersicum) indicates that there are 10 members that represent the modern, intermediate, and ancient clades. Using phylogenetic analysis and a reverse genetic approach, in this study we identified SlLAM1 in the modern clade and examined its function and tissue-specific expression pattern. We found that knocking out SlLAM1 via CRISPR/Cas9-mediated genome editing led to narrow leaves and a reduced number of secondary leaflets. Overexpression of tomato SlLAM1 could rescue the defects of the tobacco lam1 mutant. Anatomical and transcriptomic analyses demonstrated that floral organ development, fruit size, secondary leaflet initiation, and leaf complexity were altered due to loss-of-function of SlLAM1. These findings demonstrate that tomato SlLAM1 plays an important role in the regulation of secondary leaflet initiation, in addition to its conserved function in blade expansion.
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http://dx.doi.org/10.1093/jxb/eraa574DOI Listing
February 2021

Severe Acute Respiratory Syndrome Coronavirus-2 Spike Protein Nanogel as a Pro-Antigen Strategy with Enhanced Protective Immune Responses.

Small 2020 11 26;16(46):e2004237. Epub 2020 Oct 26.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Prevention and intervention methods are urgently needed to curb the global pandemic of coronavirus disease-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Herein, a general pro-antigen strategy for subunit vaccine development based on the reversibly formulated receptor binding domain of SARS-CoV-2 spike protein (S-RBD) is reported. Since the poor lymph node targeting and uptake of S-RBD by antigen-presenting cells prevent effective immune responses, S-RBD protein is formulated into a reversible nanogel (S-RBD-NG), which serves as a pro-antigen with enhanced lymph node targeting and dendritic cell and macrophage accumulation. Synchronized release of S-RBD monomers from the internalized S-RBD-NG pro-antigen triggers more potent immune responses in vivo. In addition, by optimizing the adjuvant used, the potency of S-RBD-NG is further improved, which may provide a generally applicable, safer, and more effective strategy for subunit vaccine development against SARS-CoV-2 as well as other viruses.
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http://dx.doi.org/10.1002/smll.202004237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645880PMC
November 2020

Analysis of the spillover effect of energy intensity among provinces in China based on space-time lag model.

Environ Sci Pollut Res Int 2020 May 6;27(14):16950-16962. Epub 2020 Mar 6.

School of Business Administration, Northeastern University, Shenyang, 110169, China.

Based on inter-provincial energy intensity data in China from 1996 to 2016, using the model combining STIRPAT and dynamic SDM analyzes energy intensity and its influencing factors under the conditions of spatial lag, time lag, and space-time lag. Considering endogenous issues, it then explores the basic characteristics of energy intensity in space and its path dependence. The results show that spatial distribution of energy intensity in China is uneven and generally shows a pattern of decreasing from northwest to southeast. Energy intensity itself has a significant spillover effect, which can affect neighboring regions through pollution heaven effect and pollution halo effect. It can also be reduced as a result of the joint effect of driving factors. Economic development level, foreign direct investment, and technological progress have significant effects on reducing energy intensity, while industrial structure and urbanization rate increase it. The difference among driving factors lies in spatial spillover effect, and the short-term indirect effect is greater than the long-term one. Therefore, the key to realize China mode of green development is to promote factors of reducing energy intensity brought into full play and the inhibitory factors effectively controlled.
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http://dx.doi.org/10.1007/s11356-020-08169-6DOI Listing
May 2020

Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity.

Nat Commun 2020 Jan 27;11(1):524. Epub 2020 Jan 27.

Department of Molecular Genetics & Microbiology, Duke University Medical School, Durham, NC, 27701, USA.

Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of CD8 T cell immunity; elicit Th1-promoting inflammation; and lack interference with innate or adaptive immunity. However, notorious genetic instability and underlying neuropathogenicity has hampered poliovirus-based vector applications. Here we devised a strategy based on the polio:rhinovirus chimera PVSRIPO, devoid of viral neuropathogenicity after intracerebral inoculation in human subjects, for stable expression of exogenous antigens. PVSRIPO vectors infect, activate, and induce epitope presentation in DCs in vitro; they recruit and activate DCs with Th1-dominant cytokine profiles at the injection site in vivo. They efficiently prime tumor antigen-specific CD8 T cells in vivo, induce CD8 T cell migration to the tumor site, delay tumor growth and enhance survival in murine tumor models.
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http://dx.doi.org/10.1038/s41467-019-13939-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985231PMC
January 2020

Identification and Characterization of the Tyrosinase Inhibitory Activity of Caffeine from Camellia Pollen.

J Agric Food Chem 2019 Nov 8;67(46):12741-12751. Epub 2019 Nov 8.

College of Food and Biological Engineering , Jimei University , Xiamen , Fujian 361021 , China.

Tyrosinase inhibitors are important in cosmetic, medical, and food industries due to their regulation of melanin production. A tyrosinase inhibitor was purified from Camellia pollen using high-speed countercurrent chromatography and preparative high-performance liquid chromatography and was identified as caffeine by NMR and mass spectrometry. It showed strong mushroom tyrosinase inhibitory activity with an IC of 18.5 ± 2.31 μg/mL in a noncompetitive model. The caffeine did not interact with copper ions in the active center of the enzyme but could quench fluorescence intensity and change the secondary conformation of this tyrosinase. A molecular dynamics simulation showed that caffeine bound this tyrosinase via Lys379, Lys 376, Asp357, Glu356, Thr308, Gln307, Asp312, and Trp358, thus changing the binding sites of l-tyrosine and the loop conformation adjacent to the active center. In vitro cell model analysis revealed that caffeine exhibited significant inhibitory effects on both intracellular tyrosinase activity and melanin production of B16-F10 melanoma cells in a concentration-dependent manner. These comprehensive results suggest that caffeine is a strong tyrosinase inhibitor that has the potential to be developed as skin-whitening agents in the cosmetics and pharmaceutical industries or as antibrowning agents in the food industry.
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http://dx.doi.org/10.1021/acs.jafc.9b04929DOI Listing
November 2019

Separation and purification of astaxanthin from Phaffia rhodozyma by preparative high-speed counter-current chromatography.

J Chromatogr B Analyt Technol Biomed Life Sci 2016 Sep 25;1029-1030:191-197. Epub 2016 Jun 25.

College of Food and Bioengineering, Jimei University, Xiamen, Fujian 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China; Research Center of Food Biotechnology of Xiamen City, Xiamen, Fujian 361021, China; Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen, Fujian 361021, China. Electronic address:

An effective high-speed counter-current chromatography (HSCCC) method was established for the preparative isolation and purification of astaxanthin from Phaffia rhodozyma. With a two-phase solvent system composed of n-hexane-acetone-ethanol-water (1:1:1:1, v/v/v/v), 100mg crude extract of P. rhodozyma was separated to yield 20.6mg of astaxanthin at 92.0% purity. By further one step silica gel column chromatography, the purity reached 99.0%. The chemical structure of astaxanthin was confirmed by thin layer chromatography (TLC), UV spectroscopy scanning, high performance liquid chromatography with a ZORBAX SB-C18 column and a Waters Nova-pak C18 column, and ESI/MS/MS.
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http://dx.doi.org/10.1016/j.jchromb.2016.06.042DOI Listing
September 2016

Benign pineal cyst lined with normal choroid plexus mimicking tumour in a young girl with exotropia.

BMJ Case Rep 2016 Jan 25;2016. Epub 2016 Jan 25.

Department of Neurosciences and Pediatrics, University of California San Diego, San Diego, California, USA.

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http://dx.doi.org/10.1136/bcr-2015-214170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735152PMC
January 2016

miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients.

J Neurooncol 2015 Jun 23;123(2):205-16. Epub 2015 Apr 23.

Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.

Analysis of extracellular vesicles (EVs) derived from plasma or cerebrospinal fluid (CSF) has emerged as a promising biomarker platform for therapeutic monitoring in glioblastoma patients. However, the contents of the various subpopulations of EVs in these clinical specimens remain poorly defined. Here we characterize the relative abundance of miRNA species in EVs derived from the serum and cerebrospinal fluid of glioblastoma patients. EVs were isolated from glioblastoma cell lines as well as the plasma and CSF of glioblastoma patients. The microvesicle subpopulation was isolated by pelleting at 10,000×g for 30 min after cellular debris was cleared by a 2000×g (20 min) spin. The exosome subpopulation was isolated by pelleting the microvesicle supernatant at 120,000×g (120 min). qRT-PCR was performed to examine the distribution of miR-21, miR-103, miR-24, and miR-125. Global miRNA profiling was performed in select glioblastoma CSF samples. In plasma and cell line derived EVs, the relative abundance of miRNAs in exosome and microvesicles were highly variable. In some specimens, the majority of the miRNA species were found in exosomes while in other, they were found in microvesicles. In contrast, CSF exosomes were enriched for miRNAs relative to CSF microvesicles. In CSF, there is an average of one molecule of miRNA per 150-25,000 EVs. Most EVs derived from clinical biofluids are devoid of miRNA content. The relative distribution of miRNA species in plasma exosomes or microvesicles is unpredictable. In contrast, CSF exosomes are the major EV compartment that harbor miRNAs.
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http://dx.doi.org/10.1007/s11060-015-1784-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459648PMC
June 2015

Investigation of sunlight-induced deterioration of aroma of pummelo (Citrus maxima) essential oil.

J Agric Food Chem 2014 Dec 2;62(49):11818-30. Epub 2014 Dec 2.

College of Bioengineering, Jimei University , Xiamen, Fujian Province 361021, People's Republic of China.

Deterioration of aromas of pummelo essential oil (EO) induced by sunlight was compared to those induced by heat and oxygen exposure using the techniques of sensory evaluation and GC-MS analysis. The sunlight-exposed EO was found to possess an oily off-flavor odor, which was significantly different from its counterparts induced by oxygen and heat. The strong oily note of the sunlight-exposed EO was attributed to the existence of linalool oxides and limonene oxides, as well as the lack of neral and geranial, for which UV sunlight was revealed to be the critical contributor causing the chemical reactions for the aroma changes. The results demonstrated that UV sunlight could significantly affect the aroma of the pummelo EO, providing valuable information that will benefit the production and storage of EO-based aromatic products.
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http://dx.doi.org/10.1021/jf504294gDOI Listing
December 2014