Publications by authors named "Yuan Zhao"

783 Publications

Efficacy of erythropoietin alone in treatment of neonates with hypoxic-ischemic encephalopathy: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26365

Neonatal Medicine, Shanxi Children's Hospital, Shanxi, China.

Background: Multiple clinical trials have demonstrated the safety and efficacy of erythropoietin in improving neurodevelopmental outcomes in infants with hypoxic-ischemic encephalopathy (HIE). It is undoubtedly urgent to include only randomized controlled trials (RCTs) for more standardized systematic reviews and meta-analyses. The purpose of this study is to examine whether erythropoietin reduces the risk of death and improve neurodevelopmental disorders in infants with HIE.

Methods: The electronic databases of Cochrane Library, EMBASE, PubMed, and Web of Science were searched from the inception to June 2021 using the following key terms: "erythropoietin," "hypoxic-ischemic encephalopathy," and "prospective," for all relevant RCTs. Only English publications were included. The primary outcome was mortality rate. Secondary outcomes included neurodevelopmental disorders, brain injury, and cognitive impairment. The Cochrane risk of bias tool was independently used to evaluate the risk of bias of included RCTs by 2 reviewers.

Results: We hypothesized that group with erythropoietin would provide better therapeutic benefits compared with control group.

Osf Registration Number: 10.17605/OSF.IO/FERUS.
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http://dx.doi.org/10.1097/MD.0000000000026365DOI Listing
June 2021

Surface-Enhanced Raman Scattering-Active Plasmonic Metal Nanoparticle-Persistent Luminescence Material Composite Films for Multiple Illegal Dye Detection.

Anal Chem 2021 Jun 14. Epub 2021 Jun 14.

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, International Joint Research Center for Photoresponsive Molecules and Materials, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.

Uniform two-dimensional plasmonic nanoparticle (NP)-semiconductor composite films could retard the attenuation of electromagnetic evanescent wave and show intensive Raman activity for the multiplex monitoring of hazards in a practical food matrix. Here, an efficient Raman platform is developed by employing a plasmonic nanoparticle (NP)-persistent luminescence material (PLM) composite film. PLM show upconversion photoluminescence (UCPL) properties. The emitted photons are absorbed by plasmonic NPs, which further boost the surface plasmon resonance for the generation of high polarizability and induce strong electromagnetic strength for surface-enhanced Raman scattering (SERS) enhancement. A UCPL-assisted SERS-enhanced mechanism is proposed and verified. A plasmonic NP-PLM film with superior SERS activity and detection capability becomes an alternative candidate for the sensitive and multiple detection of illegal addition of dyes in a food matrix. The proposed UCPL-assisted SERS-enhanced mechanism provides promising future directions to this end to design a next-generation SERS-active plasmonic NP-PLM composite film for the specific detection in complex samples.
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http://dx.doi.org/10.1021/acs.analchem.1c01442DOI Listing
June 2021

The Deubiquitinase USP39 Promotes ESCC Tumorigenesis Through Pre-mRNA Splicing of the mTORC2 Component Rictor.

Front Oncol 2021 26;11:667495. Epub 2021 May 26.

Biology Department, School of Life Sciences, Anhui Medical University, Hefei, China.

Spliceosomes are large RNA-protein molecular complexes which mediate splicing of pre-mRNA in eukaryotic cells. Their function is frequently altered in cancer, providing opportunities for novel therapeutic approaches. The ubiquitin specific protease 39 (USP39) is a highly conserved deubiquitylation family member that plays an essential role in pre-mRNA splicing where it serves to assemble the mature spliceosome complex. Previous studies have reported that USP39 acts in an oncogenic manner where it contributes to cancer progression and predicts poor prognosis in various human tumor types. Here we report that USP39 is differentially upregulated in human esophageal squamous cell carcinoma (ESCC) and its expression is significantly associated with clinicopathological characteristics including differentiation status and TNM stage. We found the USP39 upregulation was maintained in ESCC cell lines where it functioned to promote cancer cell growth and in xenografts. RNA-seq analyses identified that mTOR pathway activation was affected by shRNA-mediated silencing of USP39. Subsequent biochemical analyses demonstrated that USP39 regulates the activity of mTORC2 by selectively enhancing the splicing and maturation of Rictor mRNA, although not other key mTORC components. Together, our report proposes USP39 as a biomarker and oncogenic factor in ESCC, with a potential for targeting the USP39/mTOR2/Rictor axis as a therapeutic strategy. Furthermore, our study adds ESCC to the list of cancers where USP39 contributes to tumorigenesis and progression.
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http://dx.doi.org/10.3389/fonc.2021.667495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189149PMC
May 2021

A commentary on "Preventive intramuscular phenylephrine in elective cesarean section under spinal anesthesia: A randomized controlled trial" [Int. J. Surg. 62 (2019) 5-11].

Int J Surg 2021 Jun 27;90:105977. Epub 2021 May 27.

Department of Gynaecology, Gansu Provincial Maternity and Child-care Hospital, Lanzhou City, 730050, Gansu, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijsu.2021.105977DOI Listing
June 2021

Synthesis and Characterizations of a Plutonium(III) Crown Ether Inclusion Complex.

Inorg Chem 2021 May 28. Epub 2021 May 28.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, P. R. China.

We report the synthesis, single-crystal structure, solid-state ultraviolet-visible-near-infrared spectroscopy, and theoretical calculations on the first trivalent plutonium crown ether inclusion complex, [(HO)(18-crown-6)][Pu(HO)(18-crown-6)](ClO)·2(HO) (denoted as Pu-18C6). Single-crystal X-ray diffraction reveals that Pu-18C6 crystallizes in the orthorhombic space group of , which is assembled by independent ionic pairs including [Pu(HO)(18-crown-6)], [(HO)(18-crown-6)], and perchlorate anions. The plutonium atom is fully encapsulated within the cavity of the 18-crown-6, generating a distorted bicapped square antiprism geometry. The theoretical evaluation confirms that weak Pu-O dative bond is involved between Pu ions with 18-crown-6. This work may deepen the understanding of the host-guest interactions between trivalent transuranic and macrocyclic ligands.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00886DOI Listing
May 2021

Effect of bilateral internal thoracic artery harvesting on deep sternal wound infection in diabetic patients: Review of literature.

Ann Med Surg (Lond) 2021 Jun 7;66:102382. Epub 2021 May 7.

Department of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, 139 Renmin Middle Rd, Changsha, 410011, China.

Instead of its documented superiority of patency and long-term outcomes, the bilateral internal thoracic artery grafts are underused in the general population, and its use is controversial and debatable in diabetic patients due to long surgery duration, post-surgical bleeding, and sternal wound complications such as sternal wound infection, mediastinitis, and sternal wound dehiscence. This review article is particularly focused on deep sternal wound infection (DSWI) of bilateral internal thoracic artery (BITA) grafts in diabetic patients with comparison to single internal thoracic artery (SITA) graft.
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http://dx.doi.org/10.1016/j.amsu.2021.102382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138484PMC
June 2021

Design and Evaluation of Scholarship and Research Orientation Sessions in Academic Pharmacy Practice Experiences.

J Med Educ Curric Dev 2021 Jan-Dec;8:23821205211014897. Epub 2021 May 5.

Sullivan University College of Pharmacy and Health Sciences, Louisville, KY, USA.

Objective: Evaluate the efficacy of educating academic Advance Pharmacy Practice Experiential (APPE) students on scholarship and research (S&R). We hypothesized that academic APPE students gain more knowledge in scholarship and research in comparison to non-academic APPE students.

Methods: As a component of academic APPE rotation, all academic APPE students assigned by the office of experiential education per year (the intervention group) (n = 11) attended S&R sessions during the first week of their academic APPE rotation. Student volunteers from the same graduating class were recruited as the control group (n = 12). However, 1 student from the intervention group and 2 students from the control group were excluded from the analysis, leaving 10 students from each group. These sessions were taken in small groups of 1 to 3 once in their assigned rotation. S&R sessions included many topics that were based on a S&R manual. Upon completion of the sessions, a hard copy of a 10-question knowledge-based quiz was given to both groups at the same time. APPE students' knowledge about S&R were assessed via grading the quiz. The anonymity of the quiz was maintained by requesting students only to mention whether they are from intervention group or controlled group.

Results: While control group averaged 49% ± 4.58 on the quiz, the intervention group averaged 71.5% ± 5.77. Statistical analysis was performed using SPSS software. Based on independent sample -test, students who took the S&R session show significant increase in their quiz score.

Conclusion: Compared to the control group, S&R sessions helped academic APPE students to gain knowledge on S&R. The students also perceived that S&R sessions increased their interest in S&R.
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http://dx.doi.org/10.1177/23821205211014897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114237PMC
May 2021

Complete Genome Sequence of Tsuneonella flava SS-21NJ, a Potential Oil Sludge Bioremediation Agent.

Microbiol Resour Announc 2021 May 20;10(20). Epub 2021 May 20.

School of Environmental and Safety Engineering, Changzhou University, Changzhou, China

We report here the complete genome sequence of strain SS-21NJ, which was isolated from oil sludge from Shengli Oilfield in Dongying, Shandong Province, China. These results provide basic information for functional genomics and oil degradation research of strains.
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http://dx.doi.org/10.1128/MRA.00216-21DOI Listing
May 2021

Combination of multiple computational methods revealing specific sub-sectional recognition and hydrogen-bond dependent transportation of CKII peptide fragment in O-GlcNAc transferase.

Comput Struct Biotechnol J 2021 8;19:2045-2056. Epub 2021 Apr 8.

The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, People's Republic of China.

O-linked β-N-acetyl-D-glucosamine (O-GlcNAc) transferase (OGT) is an essential enzyme in many cellular physiological catalytic reactions that regulates protein O-GlcNAcylation. Aberrant O-GlcNAcylation is related to insulin resistance, diabetic complications, cancer and neurodegenerative diseases. Understanding the peptide delivery in OGT is significant in comprehending enzymatic catalytic process, target-protein recognition and pathogenic mechanism. Herein extensive molecular dynamics (MD) simulations combined with various techniques are utilized to study the recognizing and binding mechanism of peptide fragment extracted from casein kinase II by OGT from atomic level. The residues of His496, His558, Thr633, Lys634, and Pro897 are demonstrated to play a dominant role in the peptide stabilization via hydrogen bonds and σ-π interaction, whose van der Waals and non-polar solvent effects provide the main driving force. In addition, two channels are identified. The delivery mode, mechanism together with thermodynamic and dynamic characterizations for the most favorable channel are determined. The peptide is more inclined to be recognized by OGT through the cavity comprised of residues 799-812, 893-899, and 865-871, and Tyr13-terminal is prior recognized to Met26-terminal. The transportation process is accompanied with conformation changes between the "spread" and "V" shapes. The whole process is strong exothermic that is highly dependent on the variation of hydrogen bond interactions between peptide and OGT as well as the performance of different subsections of peptide. Besides that, multiple computational methods combinations may contribute meaningfully to calculation of similar bio-systems with long and flexible substrate.
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http://dx.doi.org/10.1016/j.csbj.2021.04.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085782PMC
April 2021

Salvianolic acid B protects against MPP+-induced neuronal injury via repressing oxidative stress and restoring mitochondrial function.

Neuroreport 2021 Jun;32(9):815-823

Department of Geriatrics, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Maintaining normal conditions in the mitochondria and repressing oxidative stress has emerged as a crucial therapeutic strategy to ameliorate neuron damage in Parkinson's disease. Salvianolic acid B (SalB) is a polyphenolic compound isolated from Salvia miltiorrhiza, which has been prescribed for various biological properties, including antioxidative stress, anti-inflammation and neuroprotection in pathological conditions. Previously, SalB was reported to be of benefit in slowing Parkinson's disease pathology, but whether the neuroprotective role of SalB is associated with a mitochondrial protective action is still elusive. Here we aimed to explore the effects of SalB on mitochondrial function in Parkinson's disease to uncover the underlying cellular mechanisms. The results showed that SalB significantly alleviated 1-methyl-4-phenylpyridinium (MPP+)-induced mitochondrial disruption in line with ameliorated oxidative injury, which is evidenced by inhibited mitochondrial membrane potential collapse, reduced reactive oxygen species (ROS) generation, increased expression of NAD(P)H: quinone oxidoreductase, and enhanced mitochondrial biosynthesis - the upregulation of nuclear respiratory factor 1 and mitochondrial transcription factor A expressions. Mechanistically, SalB not only increased AMP-activated protein kinase (AMPK) activation and sirtuin3 mRNA and protein levels, but also attenuated ROS-triggered neuroinflammation by downregulating the expressions of NOD-like receptor family pyrin domain containing 3, caspase-1 and Interleukin-1β (IL-1β). In conclusion, these in-vitro findings, for the first time, demonstrate that SalB offers protection against MPP+-induced neuronal injury via upregulating sirtuin3 expression and activating the AMPK signaling to restore mitochondrial function.
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http://dx.doi.org/10.1097/WNR.0000000000001660DOI Listing
June 2021

Vector spatiotemporal solitons in cold atomic gases with linear and nonlinear PT symmetric potentials.

Opt Express 2021 Apr;29(9):14016-14024

Realizing vector spatiotemporal solitons that are stable in high dimensions is a long-standing goal in the study of nonlinear optical physics. Here, a scheme is proposed to generate three-dimensional (3D) vector spatiotemporal solitons in a cold atomic system with linear and nonlinear parity-time (PT) potentials by utilizing electromagnetically induced transparency (EIT). We investigate the existence and stability of these vector 3D semilunar solitons (SSs) and vortex solitons (VSs) supported by the linear and nonlinear PT potentials. The results show that these solitons have extremely low generation power and very slow propagation velocity and can stably propagate with constant total energy in this system. The frontal head-on collisions of two vector solitons feature quasi-elastic collisions. The dynamics characteristics of these solitons depend on the linear and nonlinear PT-symmetric potential parameters, in particular, the imaginary part of PT potentials. Our study provides a new route for manipulating high-dimensional nonlinear vector optical signals via the controlled optical linear and nonlinear potentials in cold atomic gases.
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http://dx.doi.org/10.1364/OE.426056DOI Listing
April 2021

Activity and structure of methanogenic microbial communities in sediments of cascade hydropower reservoirs, Southwest China.

Sci Total Environ 2021 Sep 4;786:147515. Epub 2021 May 4.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China. Electronic address:

Freshwater reservoirs are an important source of the greenhouse gas methane (CH). However, little is known about the activity and structure of microbial communities involved in methanogenic decomposition of sediment organic matter (SOM) in cascade hydropower reservoirs. In this study, we targeted on sediments of three cascade reservoirs in Wujiang River, Southwest China. Our results showed that the content of sediment organic carbon (SOC) was between 3% and 11%, and it's positively correlated with both C/N ratio and recalcitrant organic carbon content of SOM. Meanwhile, SOC content was positively correlated with CH production rates but had no significant correlation with total CO production rates of the sediments, when rates were normalized to sediment volume. Resultantly, the sediment anaerobic decomposition rates hardly significantly increase along with the SOC content. These results suggested that the terrestrial organic matter accumulated after damming stimulated CH production from the reservoir sediments even though its decomposition rate was limited. Meantime, high throughput sequencing of 16S rRNA genes indicated that not only the hydrogenotrophic and acetoclastic, but also the methylotrophic methanogens (Methanomassiliicoccus) are abundant in the reservoir sediments. Moreover, metagenomic sequencing also suggested that methylotrophic methanogenesis are potentially important in the sediment of cascade reservoirs. Finally, the hydraulic residence time of the reservoir could be the key controlling factor of the structures of bacterial and archaeal communities as well as the CH production rates of the reservoir sediments.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147515DOI Listing
September 2021

Whole-genome resequencing identified candidate genes associated with the number of ribs in Hu sheep.

Genomics 2021 May 11;113(4):2077-2084. Epub 2021 May 11.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China. Electronic address:

The number of ribs is an important economic trait in the sheep industry when the sheep are raised for mutton. However, in sheep, the genetic mechanisms regulating rib number are poorly understood. In the present study, we aimed to identify important candidate genes that affect the increase in rib number in sheep. Whole-genome resequencing of 36 Hu sheep with an increased number of ribs (R14) and 36 sheep with normal (R13) rib numbers was carried out. Analysis using three methods (fixation index (F), Fisher's exact test, and Chi-squared test) showed that 219 single nucleotide polymorphism sites overlapped among the results of the three methods, which represented 206 genes. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that the genes were mainly associated with regulation of developmental process, inorganic anion transport, cellular biosynthetic process, tight junction, the oxytocin signaling pathway, and arrhythmogenic right ventricular cardiomyopathy. Four mutations were selected according to the significantly selected genomic regions and important pathways for genotyping and association analysis. The result demonstrated that three synonymous mutations correlated significantly with the rib number. Importantly, we revealed that the CPOX (encoding coproporphyrinogen oxidase), KCNH1 (encoding potassium voltage-gated channel subfamily H member 1), and CPQ (encoding carboxypeptidase Q) genes have a combined effect on rib number in Hu sheep. Our results identified candidate molecular markers for rib number in sheep breeding.
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http://dx.doi.org/10.1016/j.ygeno.2021.05.004DOI Listing
May 2021

Probiotics Lactobacillus rhamnosus GG ATCC53103 and Lactobacillus plantarum JL01 induce cytokine alterations by the production of TCDA, DHA, and succinic and palmitic acids, and enhance immunity of weaned piglets.

Res Vet Sci 2021 Jul 14;137:56-67. Epub 2021 Apr 14.

College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China; Ministry of Education Laboratory of Animal PRODUCTION and Quality Security, Jilin Agricultural University, Changchun, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, China. Electronic address:

Probiotics, including Lactobacillus rhamnosus GG ATCC53103 and Lactobacillus plantarum JL01, can improve growth performance and immunity of piglets, and relieve weaning stress-related immune disorders such as intestinal infections and inflammation. This study aimed to evaluate the ability of co-administration of the probiotics L. rhamnosus GG ATCC53103 and L. plantarum JL01 to stimulate immune responses and improve gut health during the critical weaning period in piglets. Forty-eight weaned piglets were randomly divided into four groups, and fed daily for 28 days either without, or with the two probiotics independently, or in combination. On day 28, we analyzed the cytokine and bacterial changes in intestinal mucosa and the hepatic portal vein blood metabolites of the weaned piglets. Our results showed that combined L. rhamnosus GG ATCC53103 and L. plantarum JL01 significantly increased (p < 0.05) the growth performance and expression of IL-10 and TGF-β1 mRNAs. In contrast, this treatment significantly decreased (p < 0.05) IL-1β mRNA level in the jejunum, ileum, and cecum. Furthermore, the secretion of IL-6 in the cecum, IL-1β in the jejunum, ileum, and cecum, and TNF-α in the jejunum and ileum was significantly decreased (p < 0.05). The relative abundance of Prevotella_9 and Enterococcus in ileum and cecum was significantly increased (p < 0.05). The relative abundance of Ruminococcus_1 and Ruminococcaceae_UCG-005 in cecum was significantly decreased (p < 0.05). Prevotella_9 and Enterococcus may increase the accumulation of (4Z,7Z,10Z,13Z,16Z,19Z)-4,7,10,13,16,19-docosahexaenoic acid (DHA) and tauroursodeoxycholic acid (TCDA) in portal vein blood, while Ruminococcus_1 and Ruminococcaceae_UCG-005 may decrease the accumulation of succinic and palmitic acids. These results indicate that L. rhamnosus GG ATCC53103 and L. plantarum JL01 may regulate cytokine levels by reducing the accumulation of succinic and palmitic acids and increasing the accumulation of TCDA and DHA, thereby enhancing the immunity of weaned piglets.
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http://dx.doi.org/10.1016/j.rvsc.2021.04.011DOI Listing
July 2021

The olfactory route is a potential way for SARS-CoV-2 to invade the central nervous system of rhesus monkeys.

Signal Transduct Target Ther 2021 04 24;6(1):169. Epub 2021 Apr 24.

National Kunming High-Level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.

Neurological manifestations are frequently reported in the COVID-19 patients. Neuromechanism of SARS-CoV-2 remains to be elucidated. In this study, we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19. In rhesus monkey, SARS-CoV-2 invades the CNS primarily via the olfactory bulb. Thereafter, viruses rapidly spread to functional areas of the central nervous system, such as hippocampus, thalamus, and medulla oblongata. The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons, microglia, and astrocytes in the CNS. Consistently, SARS-CoV-2 infects neuro-derived SK-N-SH, glial-derived U251, and brain microvascular endothelial cells in vitro. To our knowledge, this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model, which provides important insights into the CNS-related pathogenesis of SARS-CoV-2.
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http://dx.doi.org/10.1038/s41392-021-00591-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065334PMC
April 2021

Nrf2/HO-1 Axis Regulates the Angiogenesis of Gastric Cancer via Targeting VEGF.

Cancer Manag Res 2021 12;13:3155-3169. Epub 2021 Apr 12.

Department of Pathology, Ningxia Medical University, Yinchuan City, Ningxia Province, 750004, People's Republic of China.

Purpose: Gastric cancer (GC) is one of the most fatal digestive tumors worldwide. Abnormal activation or accumulation of the nuclear factor-erythroid 2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) axis is a malignant event in numerous solid tumors. However, its involvement in angiogenesis of GC remains unknown. This study investigated the role of the Nrf2/HO-1 axis in angiogenesis of GC.

Methods: The expression of Nrf2, HO-1, and vascular endothelial growth factor (VEGF) in BGC-823 cells under hypoxia was analyzed using immunocytochemistry, immunofluorescence, Western blotting, and quantitative polymerase chain reaction. The effects of brusatol (Nrf2 inhibitor) and tert-butylhydroquinone (Nrf2 inducer) on these factors and angiogenesis were examined using immunofluorescence, Western blotting, quantitative polymerase chain reaction, and tube formation assay. Moreover, immunohistochemistry and Western blotting were used to determine these factors and microvessel density in tumor and normal tissues of tumor-bearing and tumor-free mice, respectively. Immunohistochemistry and Western blotting were employed to examine these factors and microvessel density in human paracancerous tissues, well-differentiated GC, and poorly differentiated GC. The correlations between Nrf2, HO-1, and VEGF gene expression in 375 patients with GC from The Cancer Genome Atlas cohort were analyzed.

Results: The expression of Nrf2, HO-1, and VEGF was increased in hypoxic BGC-823 cells (<0.05). Although brusatol decreased their expression and angiogenesis (<0.05), tert-butylhydroquinone had the opposite effect (<0.05). Moreover, the expression of Nrf2, HO-1, and VEGF, and microvessel density in tumor tissues was higher than that recorded in normal tissues of nude mice (<0.05). Similarly, these parameters were low in paracancerous tissues, but high in GC tissues (<0.05). Also, they were weak in well-differentiated GC, but strong in poorly differentiated GC (<0.05). In addition, there was a significant correlation between Nrf2, HO-1, and VEGF (<0.05).

Conclusion: The Nrf2/HO-1 axis may regulate the angiogenesis of GC via targeting VEGF. These findings provide a promising biomarker and potential treatment target for GC.
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http://dx.doi.org/10.2147/CMAR.S292461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055645PMC
April 2021

HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus.

Evid Based Complement Alternat Med 2021 15;2021:1072975. Epub 2021 Mar 15.

Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China.

Huo-Xue-Jiang-Tang Yin (HXJTY) is a Chinese medicine formulation, which has been widely used for the treatment of various lipometabolism- and glycometabolism-related diseases in clinics. Currently, HXJTY is mainly prescribed to treat patients with type 2 diabetes mellitus (T2DM), yet its chemical and pharmacologic profiles remain to be elucidated. Here, the potential bioactive compound and action mechanism were investigated using chemical and network pharmacology analysis. A rapid HPLC-MS was employed to identify and quantify the component of HXJTY. On the basis of the identified chemical markers from HXJTY, a network pharmacology study, including target gene prediction and functional enrichment, was applied to screen out the main quality markers of HXJTY and explore its potential mechanism for the treatment of T2DM. The results showed that a total of 22 components were identified and quantified from HXJTY by HPLC-MS. Furthermore, 12 active components such as astragaloside IV, calycosin-7-O--D-glucoside, hydroxysafflor yellow A, and others were proposed as quality markers of HXJTY for treating T2DM based on network pharmacology analysis. In addition, 125 corresponding possible therapeutic target genes of T2DM were obtained. These target genes are mainly related to peptidase activity, hydrolase activity, phosphatase activity, and cofactor binding, suggesting the involvement of PI3K-Akt, MAPK, AGE-RAGE, and Rap1 signaling pathways in HXJTY-treated T2DM. Our results may provide a useful approach to identify potential quality markers and molecular mechanism of HXJTY for treating T2DM.
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http://dx.doi.org/10.1155/2021/1072975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984925PMC
March 2021

Ultrastable and Efficient Visible-light-driven CO Reduction Triggered by Regenerative Oxygen-Vacancies in Bi O CO Nanosheets.

Angew Chem Int Ed Engl 2021 Jun 10;60(25):13840-13846. Epub 2021 May 10.

Hefei National Laboratory for Physical Science at Microscale, National Synchrotron Radiation Laboratory, CAS Centre for Excellence in Nanoscience, University of Science and Technology of China, Hefei, 230026, P. R. China.

Herein, we first design a fast low-pressure ultraviolet light irradiation strategy for easily regenerating the nearly equivalent surface vacancies. Taking the defective Bi O CO nanosheets as an example, nearly equal amount of oxygen vacancies can be regenerated under UV light irradiation. Synchrotron-radiation quasi in situ X-ray photoelectron spectra disclose the Bi sites in the O-defective Bi O CO nanosheets can act as the highly active sites, which not only help to activate CO molecules, but also contribute to stabilizing the rate-limiting COOH* intermediate. Also, in situ Fourier transform infrared spectroscopy and in situ mass spectrometry unravel the UV light irradiation contributes to accelerating CO desorption process. As a result, the O-defective Bi O CO nanosheets achieve a stability up to 2640 h over 110 cycling tests and a high evolution rate of 275 μmol g  h for visible-light-driven CO reduction to CO.
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http://dx.doi.org/10.1002/anie.202101894DOI Listing
June 2021

Population Pharmacokinetics of Vixotrigine in Healthy Volunteers and Subjects with Trigeminal Neuralgia, Painful Lumbosacral Radiculopathy and Erythromelalgia.

Eur J Drug Metab Pharmacokinet 2021 May;46(3):395-404

Ann Arbor Pharmacometrics Group, Ann Arbor, MI, USA.

Background: Vixotrigine is a voltage and use dependent sodium channel blocker currently under development for treatment of various neuropathic pain indications.

Objective: The objective of this work was to develop a population pharmacokinetic model and assess effects of various covariates on pharmacokinetic parameters of vixotrigine.

Method: Plasma concentration-time data from 12 Phase 1 or 2 studies were included in the analyses. The data were obtained following administration of single or multiple doses of vixotrigine in healthy volunteers and patients. One- and two-compartment pharmacokinetic models were evaluated as base structural pharmacokinetic models. The inclusion of selected covariates was assessed using a stepwise backward elimination approach (α = 0.001) once the base/full model was developed. The predictive ability of the model was evaluated using a visual predictive check (VPC). The final model was used to evaluate effect of covariates on exposure of vixotrigine.

Results: A total of 10,263 pharmacokinetic samples collected from 465 subjects were included in the analyses. The pharmacokinetics of vixotrigine was adequately described by a two-compartment model with two transit absorption compartments and first-order elimination. Predictability of the model was also established by VPC. The final model included covariates of age, weight and carbamazepine co-administration on clearance, weight on central volume of distribution, food on absorption rate constant and formulation and Japanese race on bioavailability. None of the covariates identified had a clinically relevant effect, as impact on area under the plasma concentration-time curve (AUC) and maximum plasma concentration (C) was within ± 25%.

Conclusion: The model characterizes the pharmacokinetics of vixotrigine well, and the exposure of vixotrigine was comparable between healthy subjects and patients. None of the covariates evaluated have a clinically relevant impact on the pharmacokinetics of vixotrigine.
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http://dx.doi.org/10.1007/s13318-021-00678-0DOI Listing
May 2021

Promoting effects of MiR-135b on human multiple myeloma cells via regulation of the Wnt/β-catenin/Versican signaling pathway.

Cytokine 2021 Jun 23;142:155495. Epub 2021 Mar 23.

Department of Pathology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. Electronic address:

MicroRNA (MiR)-135b and its mediated Wnt/β-catenin signaling pathway are involved in human malignancies. However, their roles in multiple myeloma (MM) remained poorly understood. Our study aimed to uncover their roles in MM. MiR-135b and Versican expressions were measured using quantitative real-time polymerase chain reaction (qRT-PCR). MM cell proliferation, apoptosis, migration and invasion were detected by cell counting kit-8 (CCK-8) assay, flow cytometry, wound healing assay and transwell assay, respectively. Relative expression of Wnt/β-catenin signaling pathway-related protein was quantified by Western blot. MiR-135b was upregulated in the serum of MM patients, and miR-135b upregulation promoted MM cell proliferation, migration and invasion but suppressed apoptosis. Also, miR-135b upregulation promoted activation of Wnt/β-catenin signaling pathway. However, downregulation of miR-135b caused an opposite effect. After incubating cells with miR-135b inhibitor and Wnt/β-catenin signaling pathway agonist Lithium chloride (LiCl), which reversed the effects of downregulating miR-135b. Versican is the downstream effector of the Wnt/β-catenin signaling pathway, and its silencing reversed the effects of LiCl on MM cells. In conclusion, miR-135b and its mediated Wnt/β-catenin signaling pathway promoted proliferation, migration and invasion but suppressed apoptosis of MM cells through regulating Versican, providing a possible treatment for MM.
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http://dx.doi.org/10.1016/j.cyto.2021.155495DOI Listing
June 2021

Nigrospora oryzae Causing Panicle Branch Rot Disease on Oryza sativa (rice).

Plant Dis 2021 Mar 17. Epub 2021 Mar 17.

China National Rice Research Institute, 28 Shuidaosuo Road, Fuyang, hangzhou, China, 311401;

The panicle branch, which is the key node for transport of photosynthesis products from source to sink, is vulnerable to many diseases caused by fungal pathogens, such as Magnaporthe oryzae, Cochliobolus miyabeanus. Among these diseases, rice blast is the most important one which causes devastating losses in many regions. In 2019 and 2020, panicle branch rot of rice with a symptom which could be mistaken with rice blast was observed in a paddy field, where is not traditional epidemical region of rice blast, in Fuyang, Zhejiang province. In 2020, similar symptom was also observed in Hubei and Anhui Province. In a paddy field in Fuyang, the symptom appeared on more than 30% investigated panicles. Diseased panicle exhibited brown to black lesions on primary or secondary branches as well as pedicels, however the grain and the neck of spike could not be infected which is the most obvious difference with rice blast. Obviously, the disease can't destroy the entire function of branch and blank grain was rarely observed, so its damage is not comparable with neck blast. Normally, it caused incomplete grain filing commonly leading to 5% - 25% grain weight loss. During the booting stage of rice, local solar irradiation time and temperature were fewer and lower than common years which may be responsible for losses caused by this disease. After surface sterilized, lesion parts cut from infected branches from 25 panicle samples were cultured on 2% water agar at 28℃ for 24-28 h, and fungi were isolated and purified by mycelial tip transferring. Among 31 isolates, 26 showed similar cultural characters. The wool-like mycelia were luxuriant and grew rapidly on PDA spreading the whole 9 cm petri dish in less than a week at 28 ℃. The mycelia were white to ashen at beginning and gradually turned black from center of the plate after 5 days culture at 28 ℃. Hyphae were smooth, branched, septate, hyaline or pale brown. Conidia were single-celled, black, spherical to subspherical, and 10.2 to 14.6 × 12.2 to 15.7 μm (n=50) in dimension and born on tip of hyaline and ampulliform conidiophores. The fungus showed similar morphological characteristics with Nigrospora oryzae (1). ITS sequences of 6 representative strains of the fungus were amplified, sequenced with primer pair, ITS1/ITS4 (2), and submitted into GenBank with an accession number, MW228165. Phylogenetic analysis was conducted with sequences of reference strains (3). The result showed that the fungus obtained in this study was fallen into the same group with N.oryzae. In view of above both morphological and molecular analysis, the strains were finally identified as N. oryzae. Pathogenicity tests were conducted in triplicate with rice panicles in initial heading stage. Fifty panicles were wounded on branches with needles and inoculated by spreading the conidia suspension (10μl, 1 × 106 conidia ml-1) on the wounds. The panicles used as control were treated in same way with 10μl of sterile water. The inoculated and control plants were kept in dark, 25 ℃ and relative humidity of more than 85% for 24 h in culture chamber. Symptoms appeared on 44 of 50 inoculated panicles which were basically similar with those observed in paddy field, while negative controls remained symptomless. The fungi re-isolated from inoculated panicles were also confirmed as N. oryzae by both morphological and molecular analysis. To the best of our knowledge, this is the first report of N. oryzae causing panicle branch rot disease on Oryza sativa (rice). This disease not only cause yield losses and lower milling quality, but could also be mistaken as rice blast incurring unnecessary fungicides spray.
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http://dx.doi.org/10.1094/PDIS-11-20-2423-PDNDOI Listing
March 2021

Comparative analyses of the soft tissue interfaces around teeth and implants: Insights from a pre-clinical implant model.

J Clin Periodontol 2021 05 13;48(5):745-753. Epub 2021 Mar 13.

Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA.

Aim: To evaluate the similarities and differences in barrier function of a peri-implant epithelium (PIE) versus a native junctional epithelium (JE).

Materials And Methods: A mouse model was used wherein titanium implants were placed sub-occlusally in healed extraction sites. The PIE was examined at multiple timepoints after implant placement, to capture and understand the temporal nature of its assembly and homeostatic status. Mitotic activity, hemidesmosomal attachment apparatus, and inflammatory responses in the PIE were compared against a JE. Additionally, we evaluated whether the PIE developed a Wnt-responsive stem cell niche like a JE.

Results: The PIE developed from oral epithelium (OE) that had, by the time of implant placement, lost all characteristics of a JE. Compared with a JE, an established PIE had more proliferating cells, exhibited lower expression of attachment proteins, and had significantly more inflammatory cells in the underlying connective tissue. Wnt-responsive cells in the OE contributed to an initial PIE, but Wnt-responsive cells and their descendants were lost as the PIE matured.

Conclusions: Although histologically similar, the PIE lacked a Wnt-responsive stem cell niche and exhibited characteristics of a chronically inflamed tissue. Both features contributed to suboptimal barrier functions of the PIE compared with a native JE.
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http://dx.doi.org/10.1111/jcpe.13446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085107PMC
May 2021

Identification of active and inactive agonists/antagonists of estrogen receptor based on Tox21 10K compound library: Binomial analysis and structure alert.

Ecotoxicol Environ Saf 2021 May 9;214:112114. Epub 2021 Mar 9.

Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, UF Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, University of Florida, Gainesville, FL 32611, USA.

Endocrine disrupting chemicals can mimic, block, or interfere with hormones in organisms and subsequently affect their development and reproduction, which has raised significant public concern over the past several decades. To investigate (quantitative) structure-activity relationship, 8280 compounds were compiled from the Tox21 10K compound library. The results show that 50% activity concentrations of agonists are poorly related to that of antagonists because many compounds have considerably different activity concentrations between the agonists and antagonists. Analysis on the chemical classes based on mode of action (MOA) reveals that estrogen receptor (ER) is not the main target site in the acute toxicity to aquatic organisms. Binomial analysis of active and inactive ER agonists/antagonists reveals that ER activity of compounds is dominated by octanol/water partition coefficient and excess molar refraction. The binomial equation developed from the two descriptors can classify well active and inactive ER chemicals with an overall prediction accuracy of 73%. The classification equation developed from the molecular descriptors indicates that estrogens react with the receptor through hydrophobic and π-n electron interactions. At the same time, molecular ionization, polarity, and hydrogen bonding ability can also affect the chemical ER activity. A decision tree developed from chemical structures and their applications reveals that many hormones, proton pump inhibitors, PAHs, progestin, insecticides, fungicides, steroid and chemotherapy medications are active ER agonists/antagonists. On the other hand, many monocyclic/nonaromatic chain compounds and herbicides are inactive ER compounds. The decision tree and binomial equation developed here are valuable tools to predict active and inactive ER compounds.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112114DOI Listing
May 2021

Plasmon-Enhanced Electroactivity of AuRu Nanostructures for Electroanalysis Applications.

Anal Chem 2021 03 11;93(11):4944-4951. Epub 2021 Mar 11.

School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

An electrochemical sensing interface is limited by poor reproducibility and inevitable interferences present in practical applications due to the weak electrochemical signals of nanotags. This motivates the need for effective strategies to enhance the electroactivity performances of nanotags. In this contribution, a plasmon-enhanced electroactivity mechanism is proposed for AuRu-based nanostructures under illumination and applied for accurate detection of human epidermal growth factor receptor-2 (HER2). AuRu nanoparticles (NPs) harvested light energy through plasmon excitation and generated holes to participate in the electrooxidation process. The production of holes resulted in the electrooxidation signal enhancement of AuRu NPs. AuRu NPs were assembled with Au NPs using HER2 aptamers as linkers, and the plasmonic coupling between AuRu NPs and Au NPs produced an intense electromagnetic field, which further enhanced the electrooxidation signals of AuRu NPs. An AuRu-Au NP assembly-dependent electrochemical aptasensor was established for the accurate detection of HER2, and the limit of detection (LOD) was as low as 1.7 pg/mL. The plasmon-enhanced electroactivity mechanism endowed AuRu-based nanostructures with strong and noninterfering electrochemical signals for sensitive and accurate detection. This insight opens new horizons for the construction of desired electroactive nanostructures for electroanalysis applications.
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http://dx.doi.org/10.1021/acs.analchem.0c05439DOI Listing
March 2021

Chiral Recognition for Chromatography and Membrane-Based Separations: Recent Developments and Future Prospects.

Molecules 2021 Feb 21;26(4). Epub 2021 Feb 21.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, China.

With the rapid development of global industry and increasingly frequent product circulation, the separation and detection of chiral drugs/pesticides are becoming increasingly important. The chiral nature of substances can result in harm to the human body, and the selective endocrine-disrupting effect of drug enantiomers is caused by differential enantiospecific binding to receptors. This review is devoted to the specific recognition and resolution of chiral molecules by chromatography and membrane-based enantioseparation techniques. Chromatographic enantiomer separations with chiral stationary phase (CSP)-based columns and membrane-based enantiomer filtration are detailed. In addition, the unique properties of these chiral resolution methods have been summarized for practical applications in the chemistry, environment, biology, medicine, and food industries. We further discussed the recognition mechanism in analytical enantioseparations and analyzed recent developments and future prospects of chromatographic and membrane-based enantioseparations.
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http://dx.doi.org/10.3390/molecules26041145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924630PMC
February 2021

Soil carbon accumulation with increasing temperature under both managed and natural vegetation restoration in calcareous soils.

Sci Total Environ 2021 May 22;767:145298. Epub 2021 Jan 22.

Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang 547100, China. Electronic address:

Vegetation restoration has been proposed as an effective strategy for increasing soil organic carbon (SOC) sequestration. However, the responses of SOC to managed and natural vegetation restoration strategies at a large scale are poorly understood due to the varying SOC components and changing climatic conditions. Here, we measured bulk SOC, particulate organic carbon (POC), and mineral-associated organic carbon (MOC) after 15 years of vegetation restoration along an elevation gradient with a corresponding temperature gradient in the calcareous soils of karst region, Southwest China. We compared managed plantation forest and naturally recovered shrubland vegetation restoration strategies, using cropland and mature forest as references. Overall, we found that the SOC and POC densities in both plantation forest and shrubland were significantly higher than in the cropland but lower than in the mature forest. There were no significant differences in the SOC pool between the plantation forest and shrubland. Furthermore, the relative changes in the SOC and POC densities increased with increasing mean annual temperature in the plantation forest and shrubland. Our results showed that both vegetation restoration strategies, characterized by higher soil microbial abundance and exchangeable Ca concentration, were beneficial to POC but not MOC accumulation, and sufficiently compensated SOC decomposition at lower elevation with higher MAT. Our results highlight the potential of both vegetation restoration strategies for promoting SOC accumulation in warmer karst regions and emphasize the necessity to understand soil carbon stabilization mechanisms in calcareous soils.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145298DOI Listing
May 2021

Bioequivalence of intramuscular and subcutaneous peginterferon beta-1a: results of a phase I, open-label crossover study in healthy volunteers.

Ther Adv Neurol Disord 2021 22;14:1756286420975227. Epub 2021 Jan 22.

Biogen, 225 Binney St., Cambridge, MA 02142, USA.

Background: Peginterferon beta-1a administered every 2 weeks subcutaneous (SC) injection is approved to treat adult patients with relapsing-remitting multiple sclerosis (RRMS) and relapsing forms of multiple sclerosis (RMS). However, associated injection site reactions (ISRs) can lead to treatment discontinuation. Prior studies with interferon beta-1a reported a lower frequency of ISRs with intramuscular (IM) administration than with SC administration. IM administration of peginterferon beta-1a may therefore represent a useful alternative treatment option.

Methods: A phase I, open-label, two-period crossover study randomized healthy volunteers to receive a single dose of peginterferon beta-1a 125 mcg administered IM followed by a single 125 mcg dose administered SC after a 28-day washout or vice versa. Blood samples were collected up to 504 h post dose to determine pharmacokinetic (PK) and pharmacodynamic (PD) profiles. The primary endpoint was assessment of bioequivalence based on maximum serum concentration (C) and area under the curve from time zero extrapolated to infinity (AUC). Other PK parameters, as well as PD (serum neopterin) and safety profiles, were also evaluated.

Results: The study enrolled 136 participants. Bioequivalence of IM and SC peginterferon beta-1a was established for both C ([least squares (LS)] mean IM/SC ratio: 1.083 [90% confidence interval (CI), 0.975-1.203]) and AUC (LS mean IM/SC ratio: 1.089 [90% CI, 1.020-1.162]). Other PK and PD parameters were similar between administration routes, although moderate to high inter-subject variability was observed for IM and SC. Safety profiles were generally balanced between IM and SC administration. ISRs occurred at a lower frequency with IM [14.4% (95% CI, 8.89-21.56%)] than with SC [32.1% (95% CI, 24.29-40.70%)] administration ( = 0.0005).

Conclusions: These results demonstrate bioequivalence between peginterferon beta-1a IM and SC and support the consideration of IM injection of peginterferon beta-1a as a viable treatment option in patients with RRMS and RMS.
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http://dx.doi.org/10.1177/1756286420975227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883310PMC
January 2021

Short chain fatty acids inhibit endotoxin-induced uveitis and inflammatory responses of retinal astrocytes.

Exp Eye Res 2021 May 20;206:108520. Epub 2021 Feb 20.

Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, USA. Electronic address:

Short chain fatty acids (SCFAs) are produced by gut microbiota as fermentation products of digestion-resistant oligosaccharides and fibers. Their primary roles are functioning as major energy sources for colon cells and assisting in gut homeostasis by immunomodulation. Recent evidence suggests that they affect various organs both at cellular and molecular levels, and regulate functions in distance sites including gene expression, cell proliferation, cell differentiation, apoptosis and inflammation. In this study, we examined whether SCFAs are present in the mouse eye and whether SCFAs affect inflammatory responses of the eye and retinal astrocytes (RACs). We observed that intra-peritoneal injected SCFAs were detected in the eye and reduced intraocular inflammation induced by lipopolysaccharide (LPS). Moreover, SCFAs displayed two disparate effects on LPS-stimulated RACs - namely, cytokine and chemokine production was reduced, but the ability to activate T cells was enhanced. Our results support the existence of gut-eye cross talk and suggest that SCFAs can cross the blood-eye-barrier via the systemic circulation. If applied at high concentrations, SCFAs may reduce inflammation and impact cellular functions in the intraocular milieu.
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http://dx.doi.org/10.1016/j.exer.2021.108520DOI Listing
May 2021

Mutation of daf-2 extends lifespan via tissue-specific effectors that suppress distinct life-limiting pathologies.

Aging Cell 2021 03 20;20(3):e13324. Epub 2021 Feb 20.

Research Department of Genetics, Evolution and Environment, Institute of Healthy Ageing, University College London, London, UK.

In aging Caenorhabditis elegans, as in higher organisms, there is more than one cause of death. C. elegans exhibit early death with a swollen, infected pharynx (P death), and later death with pharyngeal atrophy (p death). Interventions that alter lifespan can differentially affect frequency and timing of each type of death, generating complex survival curve shapes. Here, we use mortality deconvolution analysis to investigate how reduction of insulin/IGF-1 signaling (IIS), which increases lifespan (the Age phenotype), affects different forms of death. All daf-2 insulin/IGF-1 receptor mutants exhibit increased lifespan in the p subpopulation (p Age), while pleiotropic class 2 daf-2 mutants show an additional marked reduction in P death frequency. The latter is promoted by pharyngeal expression of the IIS-regulated DAF-16 FOXO transcription factor, and at higher temperature by reduced pharyngeal pumping rate. Pharyngeal DAF-16 also promotes p Age in class 2 daf-2 mutants, revealing a previously unknown role for the pharynx in the regulation of aging. Necropsy analysis of daf-2 interactions with the daf-12 steroid receptor implies that previously described opposing effects of daf-12 on daf-2 longevity are attributable to internal hatching of larvae, rather than complex interactions between insulin/IGF-1 and steroid signaling. These findings support the view that wild-type IIS acts through multiple distinct mechanisms which promote different life-limiting pathologies, each of which contribute to late-life mortality. This study further demonstrates the utility of mortality deconvolution analysis to better understand the genetics of lifespan.
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http://dx.doi.org/10.1111/acel.13324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963334PMC
March 2021

TurboHawk plaque rotation system for treatment of arteriosclerosis occlusion in lower extremities: A pilot retrospective study.

Medicine (Baltimore) 2021 Feb;100(5):e23976

Department of General Surgery, Second Affiliated Hospital of Xuzhou Medical University (General Hospital of Xuzhou Mining Group), Xuzhou, Jiangsu, China.

Abstract: This pilot study retrospectively assessed the feasible efficacy of TurboHawk plaque rotation system (THPRS) for treatment of arteriosclerosis occlusion in lower extremities (AOLE).A total of 36 eligible patients with AOLE were included in this pilot retrospective study. We divided all those patients into a treatment group and a control group, each group 18 patients. All patients in both groups administered conventional therapy. Additionally, all patients in the treatment group received THPRS, while all patients in the control group received percutaneous transluminal angioplasty (PTA) and percutaneous transluminal stenting (PTS). All outcomes were evaluated and analyzed at 3-month after surgery.At 3-month postsurgery, there were not significant statistical differences in clinical manifestations (intermittent claudication, P = .49; resting pain, P = .28), ankle brachial index change (P = .07), 6-minute walk distance (P = .43), and complications between 2 groups.This pilot study did not show better outcome improvement of THPRS for patients with AOLE. We cautiously draw the present conclusion, because it suffers from several major restrictions. Thus, further studies with larger sample size and longer term follow-up are still needed to warrant the current conclusion.
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http://dx.doi.org/10.1097/MD.0000000000023976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870169PMC
February 2021