Publications by authors named "Yuan Yuan"

2,215 Publications

  • Page 1 of 1

mutation in rapid-onset dystonia parkinsonism: New data and genotype-phenotype correlation analysis.

Front Aging Neurosci 2022 1;14:933893. Epub 2022 Aug 1.

Department of Neurology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Rapid-onset dystonia parkinsonism (RDP) is a rare disease caused by mutation with considerable clinical heterogeneity. Increased knowledge of RDP could be beneficial in its early diagnosis and treatment.

Objective: This study aimed to summarize the gene mutation spectrum of associated with RDP, and to explore the correlation of variants with RDP clinical phenotypes.

Methods: In this study, we reported two RDP patients from a family with a novel inherited variant. Then, we reviewed and analyzed the available literature in English focused on -causative RDP. A total of 35 articles covering 15 families (59 patients) and 36 sporadic RDP cases were included in our analysis.

Results: The variant A813V (2438C>T) in found in our cases was a novel mutant. Delays in diagnosis were common, with a mean delay time of 14 years. had distinct RDP-related mutation hotspots, which consisted of exon8, 14, 17, and 18, and the most frequently occurring variants were T613M and I578S. Approximately 74.5% of patients have specific triggers before disease onset, and 82.1% of RDPs have stable symptoms within 1 month. The incidence rates of dystonia and bradykinesia are 100 and 88.1%, respectively. The onset site varied and exhibited a rostrocaudal gradient distribution pattern in 45% of patients with RDP. Approximately 63.6% of patients had mild improvement after receiving comprehensive interventions, especially in gait disturbance amelioration.

Conclusion: In patients with acute and unexplained dystonia or bradykinesia, gene screening on should be timely performed. When a diagnosis has been made, treatments that may be effective are to be attempted. Our study would be helpful for the early diagnosis and treatment of -related RDP.
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http://dx.doi.org/10.3389/fnagi.2022.933893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376385PMC
August 2022

Spatiotemporal single-cell regulatory atlas reveals neural crest lineage diversification and cellular function during tooth morphogenesis.

Nat Commun 2022 Aug 16;13(1):4803. Epub 2022 Aug 16.

Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.

Cranial neural crest cells are an evolutionary innovation of vertebrates for craniofacial development and function, yet the mechanisms that govern the cell fate decisions of postmigratory cranial neural crest cells remain largely unknown. Using the mouse molar as a model, we perform single-cell transcriptome profiling to interrogate the cell fate diversification of postmigratory cranial neural crest cells. We reveal the landscape of transcriptional heterogeneity and define the specific cellular domains during the progression of cranial neural crest cell-derived dental lineage diversification, and find that each domain makes a specific contribution to distinct molar mesenchymal tissues. Furthermore, IGF signaling-mediated cell-cell interaction between the cellular domains highlights the pivotal role of autonomous regulation of the dental mesenchyme. Importantly, we reveal cell-type-specific gene regulatory networks in the dental mesenchyme and show that Foxp4 is indispensable for the differentiation of periodontal ligament. Our single-cell atlas provides comprehensive mechanistic insight into the cell fate diversification process of the cranial neural crest cell-derived odontogenic populations.
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http://dx.doi.org/10.1038/s41467-022-32490-yDOI Listing
August 2022

Effect of Comprehensive Nursing Approach in Perioperative Stage of Patients with Hepatocellular Carcinoma Interventional Therapy.

Evid Based Complement Alternat Med 2022 5;2022:6862463. Epub 2022 Aug 5.

Department of Catheterization-Room, Tianchang Traditional Chinese Medicine Hospital, Chuzhou 239300, Anhui, China.

Objective: To examine the efficacy of a complete nursing strategy during the perioperative phase for patients undergoing interventional treatment for hepatocellular cancer.

Methods: Sixty patients who were diagnosed with liver cancer and underwent interventional therapy in our hospital between February 2019 and December 2021 were recruited in this trial. All study subjects were numbered according to the time when the patients first came to our hospital, and were equally divided into a comprehensive group and a conventional group based on the odd and even number of the last number, with 30 cases in each group. Those in the conventional group received conventional nursing care, whereas patients in the comprehensive group received comprehensive nursing care. Before and after the nursing intervention, the quality of life, pain, and patient satisfaction in both groups were compared.

Results: The quality of survival scores, including physical, emotional, role, social, and cognitive function scores of patients in the comprehensive group, were significantly higher than those in the conventional group ( < 0.05); there was no statistically significant difference in numeric rating scales (NRS) scores between the two groups compared before treatment ( > 0.05). After treatment, the NRS scores of patients in the study group were significantly lower than those of patients in the control group ( < 0.05); before the intervention, the difference between the emotional state scores of patients in the two groups was not significant and not statistically significant ( > 0.05), while the emotional state of patients in both groups improved after the intervention, and the Self-Rating Anxiety Scale (SAS) and the Self-Rating Depression Scale (SDS) scores of patients in the comprehensive group were lower than those in the conventional group ( < 0.05); the total incidence of adverse reactions in the comprehensive group (10.00%) was significantly lower than that in the conventional group (46.67%) ( < 0.05); and the total satisfaction of patients in the comprehensive group (93.33%) was significantly higher than that of patients in the conventional group (73.33%) ( < 0.05).

Conclusion: The intervention of a comprehensive nursing approach in the perioperative period for patients with liver cancer is remarkable as it can relieve patients' psychological burden and pain, ensure a smooth operation, improve patients' postoperative quality of life, and also help to reduce the risk of postoperative adverse reactions, effectively enhancing patients' satisfaction, and thus deserves to be promoted in clinical practice.
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http://dx.doi.org/10.1155/2022/6862463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374545PMC
August 2022

Acrylamide induces ferroptosis in HSC-T6 cells by causing antioxidant imbalance of the XCT-GSH-GPX4 signaling and mitochondrial dysfunction.

Toxicol Lett 2022 Aug 10. Epub 2022 Aug 10.

College of Food Science and Engineering, Jilin University, Changchun, China, 130062. Electronic address:

Acrylamide (AA) is a heat-induced food contaminant, mainly metabolized by the liver. Increasing evidences have proved that ferroptosis is linked to the pathogenesis of liver disease. In the current study, the underlying mechanism of AA-induced rat hepatic stellate (HSC-T6) cells ferroptosis was investigated by detecting changes in iron levels, expressions of ferroptosis-related proteins and indicators of mitochondrial dysfunction. The results showed that AA treatment led to iron levels increased and expressions of long-chain acyl-CoA synthase 4 (ACSL4), cyclooxygenase 2 (COX2) and ferritin heavy chain 1 (FTH1) proteins in HSC-T6 cells were all altered. Treatment with the ferroptosis inhibitor ferrostatin-1 (Fer-1) markedly reversed the impact of AA, suggesting that AA induced ferroptosis in HSC-T6 cells. Mechanistically, AA induced the onset of ferroptosis by affecting XCT-GSH-GPX4 antioxidant signaling. Moreover, AA created a peroxidative environment for ferroptosis by inducing oxidative stress in HSC-T6 cells through mitochondrial dysfunction, as evidenced by increased mitochondrial ROS (mtROS) release, mitochondrial membrane potential (MMP) depolarization, and decreased mitochondrial ATP. Our results indicated that AA resulted in mitochondrial dysfunction and ferroptosis, and dysregulation of XCT-GSH-GPX4 antioxidant signaling was a key factor in AA-induced ferroptosis.
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http://dx.doi.org/10.1016/j.toxlet.2022.08.007DOI Listing
August 2022

Urinary excretion of silibinin diastereoisomers and their conjugated metabolites in rat and human at different dosages.

Biomed Chromatogr 2022 Aug 12:e5480. Epub 2022 Aug 12.

Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, China.

Silibinin is a mixture of two flavonoid lignan silibinins A and B from the seeds of milk thistle (Silybum marianum L.). Using UPLC/Q-TOFMS, a total of 18 metabolites were identified in rat and human urine samples after oral administration of Silibinin Capsule. Furthermore, nine glucuronides and/or sulfated metabolites and two prototype compounds were simultaneously quantified in rat urine after oral administration of Silibinin Capsule at 50 and 100 mg/kg. Over a 72-h period, 27.6% and 23.3% of the silibinin were excreted in the forms of eleven metabolites in urine, among which, 5 major metabolites, including silibinin A-7-O-β-glucuronide (SA-7G), silibinin B-7-O-β-glucuronide (SB-7G), silibinin A-5-O-β-glucuronide (SA-5G), silibinin B-5-O-β-glucuronide (SB-5G) and silibinin A-20-O-glucuronide (SA-20G), accounted for 20.5% and 15.5% of the dosages separatively at doses of 50 and 100 mg/kg. These results suggested that glucuronidation at the C7-, C5- and C20- hydroxyls was the primary metabolic pathway of silibinin diastereoisomers in vivo. The present results provide helpful information for in vivo metabolism and clinical usage of Silibinin Capsule.
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http://dx.doi.org/10.1002/bmc.5480DOI Listing
August 2022

Residue substitution enhances the immunogenicity of neoepitopes from gastric cancers.

Cancer Biol Med 2021 Nov;18(4)

The School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China.

Objective: Neoantigens arising from gene mutations in tumors can induce specific immune responses, and neoantigen-based immunotherapies have been tested in clinical trials. Here, we characterized the efficacy of altered neoepitopes in improving immunogenicity against gastric cancer.

Methods: Raw data of whole-exome sequencing derived from a patient with gastric cancer were analyzed using bioinformatics methods to identify neoepitopes. Neoepitopes were modified by P1Y (the first amino acid was replaced by tyrosine) and P2L (the second amino acid was replaced by leucine). T2 binding and stability assays were used to detect the affinities between the neoepitopes and the HLA molecules, as well as the stabilities of complexes. Dendritic cells (DCs) presented with neoepitopes stimulated naïve CD8 T cells to induce specific cytotoxic T lymphocytes. ELISA and carboxyfluorescein succinimidyl ester were used to detect IFN-γ and TNF-α levels, and T cell proliferation. Perforin was detected by flow cytometry. The cytotoxicity of T cells was determined using the lactate dehydrogenase assay.

Results: Bioinformatics analysis, T2 binding, and stability assays indicated that residue substitution increased the affinity between neoepitopes and HLA molecules, as well as the stabilities of complexes. DCs presented with altered neoepitopes stimulated CD8T cells to release more IFN-γ and had a greater effect on promoting proliferation than wild-type neoepitopes. CD8T cells stimulated with altered neoepitopes killed more wild-type neoepitope-pulsed T2 cells than those stimulated with wild-type neoepitopes, by secreting more IFN-γ, TNF-α, and perforin.

Conclusions: Altered neoepitopes exhibited greater immunogenicity than wild-type neoepitopes. Residue substitution could be used as a new strategy for immunotherapy to target neoantigens.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2021.0022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610152PMC
November 2021

lncRNA NUTM2A-AS1 Targets the SRSF1/Trim37 Signaling Pathway to Promote the Proliferation and Invasion of Breast Cancer.

Comput Math Methods Med 2022 2;2022:3299336. Epub 2022 Aug 2.

Department of Thyroid and Breast Surgery, Wuhan No. 1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022 Hubei Province, China.

Method: Using the tumor database (TCGA) and analysis platform (GEPIA), NUTM2A-AS1 expression in breast cancer cases was compared with the normal cases. In addition, Kaplan-Meier curve of overall survival according to the various levels of NUTM2A-AS1 was assessed. Then, we constructed a knockdown plasmid of NUTM2A-AS1 and successfully reduced the expression function of NUTM2A-AS1 in BC cells.

Results: We found NUTM2A-AS1 could promote the malignant phenotype of proliferation and invasion of BC. In terms of mechanism research, NUTM2A-AS1 was mainly located in the cytoplasm of BC cells, which indicated that NUTM2A-AS1 may achieve its function through transcriptional or posttranscriptional regulation pathways. While knocking down NUTM2A-AS1, we detected several major molecules of the trim family. The results showed that only trim37 mRNA was significantly affected, and protein detection also showed that knockdown NUTM2A-AS1 expression could reduce the expression of trim37. The results of RIP experiments suggested that NUTM2A-AS1 played a key role by combining with SRSF1 and affecting the interaction between SRSF1 and trim37 mRNA. The stability test of mRNA also confirmed that during the knockdown of NUTM2A-AS1, the mRNA stability of trim37 decreased significantly, but this downward trend could be reversed by overexpressed SRSF1. The above results suggested that NUTM2A-AS1 could maintain the stability and expression of trim37 through SRSF1 pathway. The results of rescue experiment showed the overexpression of trim37, while knocking down NUTM2A-AS1 could reverse the decrease of proliferation and invasiveness of BC cells induced by NUTM2A-AS1 knockdown.

Conclusion: Therefore, trim37 is seen as a necessary target for NUTM2A-AS1 to exert the biological function of BC. Additionally, NUTM2A-AS1 is to regulate the malignant phenotype of BC through NUTM2A-AS1/trim37 pathway.
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http://dx.doi.org/10.1155/2022/3299336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363211PMC
August 2022

An MRI-based pelvimetry nomogram for predicting surgical difficulty of transabdominal resection in patients with middle and low rectal cancer.

Front Oncol 2022 25;12:882300. Epub 2022 Jul 25.

Department of Colorectal Surgery, Changhai Hospital, Shanghai, China.

Objective: The current work aimed to develop a nomogram comprised of MRI-based pelvimetry and clinical factors for predicting the difficulty of rectal surgery for middle and low rectal cancer (RC).

Methods: Consecutive mid to low RC cases who underwent transabdominal resection between June 2020 and August 2021 were retrospectively enrolled. Univariable and multivariable logistic regression analyses were carried out for identifying factors (clinical factors and MRI-based pelvimetry parameters) independently associated with the difficulty level of rectal surgery. A nomogram model was established with the selected parameters for predicting the probability of high surgical difficulty. The predictive ability of the nomogram model was assessed by the receiver operating characteristic (ROC) curve and decision curve analysis (DCA).

Results: A total of 122 cases were included. BMI (OR = 1.269, = 0.006), pelvic inlet (OR = 1.057, = 0.024) and intertuberous distance (OR = 0.938, = 0.001) independently predicted surgical difficulty level in multivariate logistic regression analysis. The nomogram model combining these predictors had an area under the ROC curve (AUC) of 0.801 (95% CI: 0.719-0.868) for the prediction of a high level of surgical difficulty. The DCA suggested that using the nomogram to predict surgical difficulty provided a clinical benefit.

Conclusions: The nomogram model is feasible for predicting the difficulty level of rectal surgery, utilizing MRI-based pelvimetry parameters and clinical factors in mid to low RC cases.
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http://dx.doi.org/10.3389/fonc.2022.882300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357897PMC
July 2022

High-level ertapenem resistance in Klebsiella pneumoniae is due to RamA down-regulation of ompK35 through micF.

Int J Antimicrob Agents 2022 Aug 8:106653. Epub 2022 Aug 8.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China; Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China, Shanghai, China.. Electronic address:

An ertapenem-resistant Klebsiella pneumoniae clinical isolate, KP20, without carbapenemase and negative for the efflux pump inhibition test, was resistant to ertapenem at a high level (minimal inhibitory concentration [MIC] = 64 mg/L) but susceptible to meropenem and imipenem. Second-generation sequencing was performed, and a termination mutation was found in ramR. The complementation of ramR in KP20 reduced the ertapenem MIC by 128 times (from 64 to 0.5 mg/L). The over-expression of ramA and the loss of OmpK35 were discovered in strain KP20 by quantitative reverse transcription-PCR and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Further ramA deletion in strain KP20 resulted in a 128-fold decrease in the MIC of ertapenem (from 64 mg/L to 0.5 mg/L), and the expression of ompK35 was observed in KP20∆ramA by SDS-PAGE. The complementation of ramA in KP20∆ramA led to a 45.45-fold down-regulation of the ompK35. The complementation of ompK35 in KP20 could restore the susceptibility of ertapenem (the MIC reduced from 64 to 0.25 mg/L). Further, the results of the electrophoretic mobility shift assay showed that RamA could bind to the promoter of micF. These results showed that the termination mutation in ramR resulted in the over-expression of ramA, causing the loss of OmpK35 expression through up-regulation of micF, and revealing the mechanism of ertapenem resistance only in K. pneumoniae.
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http://dx.doi.org/10.1016/j.ijantimicag.2022.106653DOI Listing
August 2022

Accurate identification of taxon-specific molecular markers in plants based on DNA signature sequence.

Mol Ecol Resour 2022 Aug 11. Epub 2022 Aug 11.

National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences (CACMS), Beijing 100700, China.

Accurate identification of plants remains a significant challenge for taxonomists and is the basis for plant diversity conservation. Although DNA barcoding methods are commonly used for plant identification, these are limited by the low amplification success and low discriminative power of selected genomic regions. In this study, we developed a k-mer-based approach, the DNA signature sequence (DSS), to accurately identify plant taxon-specific markers, especially at the species level. DSS is a constant-length nucleotide sequence capable of identifying a taxon and distinguishing it from other taxa. In this study, we performed the first large-scale study of DSS markers in plants. DSS candidates of 3,899 angiosperm plant species were calculated based on a chloroplast dataset with 4,356 assemblies. Using Sanger sequencing of PCR amplicons and high-throughput sequencing, DSSs were validated in four and 165 species, respectively. Based on this, the universality of the DSSs was over 79.38%. Several indicators influencing DSS marker identification and detection have also been evaluated, and common criteria for DSS application in plant identification have been proposed.
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http://dx.doi.org/10.1111/1755-0998.13697DOI Listing
August 2022

Effect of methylenetetrahydrofolate reductase C677T polymorphism on serum folate but not vitamin B12 levels in patients with H-type hypertension.

Mol Biol Rep 2022 Aug 11. Epub 2022 Aug 11.

Department of Pharmacy, Xinjiang Medical University Affiliated First Hospital, Ürümqi, 830011, China.

Background: Hyperhomocysteinemia (HHcy) is a common complication in Chinese hypertensive patients and associated with methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, folate, and vitamin B12 (Vit B12) status. This study evaluated the associations of MTHFR C677T polymorphism, folate, and Vit B12 with H-type hypertension.

Methods And Results: 887 eligible patients with essential hypertension were included. Patients were divided into two groups according to the Hcy level, the H-type hypertension group and the normal hypertension group. Related risk factors such as MTHFR polymorphism, folate and Vit B12 status were analyzed in the two groups. Age, gender, SBP, DBP, MTHFR C677T genotype, folate and Vit B12 differed significantly between H-type hypertension and normal hypertension groups (P < 0.05). MTHFR 677TT variant, gender, folate, and Vit B12 were independent risk factors for the occurrence of H-type hypertension. The risk for TT carriers was 8 times higher than that of CC and CT carriers [OR (95% CI) 8.248 (5.274-12.899)]. Male patients had almost fivefold higher odds than female patients [OR (95% CI) 4.923 (2.741-8.842)]. Folate level of patients with H-type hypertension decreased with the C to T substitution of MTFHR C677T gene (P < 0.05), while Vit B12 level was not associated with the gene (P > 0.05).

Conclusions: MTHFR 677TT variant, gender, folate, and Vit B12 were risk factors for the occurrence of H-type hypertension. Folate but not Vit B12 was associated with MTFHR C677T polymorphism in patients with H-type hypertension. Accordingly, the above factors may be considered in the prevention and treatment of hypertension.
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http://dx.doi.org/10.1007/s11033-022-07844-wDOI Listing
August 2022

Tax1 banding protein 1 exacerbates heart failure in mice by activating ITCH-P73-BNIP3-mediated cardiomyocyte apoptosis.

Acta Pharmacol Sin 2022 Aug 10. Epub 2022 Aug 10.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Tax1 banding protein 1 (Tax1bp1) was originally identified as an NF-κB regulatory protein that participated in inflammatory, antiviral and innate immune processes. Tax1bp1 also functions as an autophagy receptor that plays a role in autophagy. Our previous study shows that Tax1bp1 protects against cardiomyopathy in STZ-induced diabetic mice. In this study we investigated the role of Tax1bp1 in heart failure. Pressure overload-induced heart failure model was established in mice by aortic banding (AB) surgery, and angiotensin II (Ang II)-induced heart failure model was established by infusion of Ang II through osmotic minipump for 4 weeks. We showed that the expression levels of Tax1bp1 in the heart were markedly increased 2 and 4 weeks after AB surgery. Knockdown of Tax1bp1 in mouse hearts significantly ameliorated both AB- and Ang II infusion-induced heart failure parameters. On the contrary, AB-induced heart failure was aggravated in cardiac-specific Tax1bp1 transgenic mice. Similar results were observed in neonatal rat cardiomyocytes (NRCMs) under Ang II insult. We demonstrated that the pro-heart failure effect of Tax1bp1 resulted from its interaction with the E3 ligase ITCH to promote the transcription factor P73 ubiquitination and degradation, causing enhanced BCL2 interacting protein 3 (BNIP3)-mediated cardiomyocyte apoptosis. Knockdown ITCH or BNIP3 in NRCMs significantly reduced Ang II-induced apoptosis in vitro. Similarly, BNIP3 knockdown attenuated heart failure in cardiac-specific Tax1bp1 transgenic mice. In the left ventricles of heart failure patients, Tax1bp1 expression level was significantly increased; Tax1bp1 gene expression was negatively correlated with left ventricular ejection fraction in heart failure patients. Collectively, the Tax1bp1 increase in heart failure enhances ITCH-P73-BNIP3-mediated cardiomyocyte apoptosis and induced cardiac injury. Tax1bp1 may serve as a potent therapeutic target for the treatment of heart failure.• Cardiac Tax1bp1 transgene mice were more vulnerable to cardiac dysfunction under stress.• Cardiac Tax1bp1 transgene mice were more vulnerable to cardiac dysfunction under stress.• Knockout of Tax1bp1 in mouse hearts ameliorated heart failure induced by pressure overload.• Tax1bp1 interacts with the E3 ligase Itch to promote P73 ubiquitination and degradation, causing enhanced BNIP3-mediated apoptosis.• Tax1bp1 may become a target of new therapeutic methods for treating heart failure.
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http://dx.doi.org/10.1038/s41401-022-00950-2DOI Listing
August 2022

Corrosion Resistance of the GO/ZIF-8 Hybrid Loading Benzotriazole as a Multifunctional Composite Filler-Modified MgAlY Layered Double Hydroxide Coating.

Langmuir 2022 Aug 9. Epub 2022 Aug 9.

College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China.

A composite coating with good load-carrying and controlled release capabilities for the corrosion inhibitor benzotriazole (BTA) was prepared while providing active and passive corrosion protection for magnesium alloy systems. In this paper, the organic corrosion inhibitor BTA was loaded into the ZIF-8/GO hybrid (GZB), and then, the GZB composite was coated with hexadecyltrimethoxysilane (HDTMS). Then, the GZB composites carried by HDTMS were made to adhere a ternary MgAlY layered double hydroxide (LDH) coating based on microarc oxidation (MAO) coating by electrophoresis (Si-MgAlY LDH coating). The successful loading of BTA by GZB composites was verified by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Meanwhile, the Si-MgAlY LDH coating was characterized by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The potentiodynamic polarization curves show that the corrosion current density of the Si-MgAlY LDH coating reaches (2.08 ± 0.49) × 10 A/cm, which means that the Si-MgAlY LDH coating greatly improves the corrosion resistance of magnesium alloy AZ31. The Si-MgAlY LDH coating can also achieve self-healing function in harsh environments, which is attributed to the synergistic effect of passive and active protection. The composite coating is of great significance to expand the potential applications of magnesium alloys.
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http://dx.doi.org/10.1021/acs.langmuir.2c01915DOI Listing
August 2022

Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice.

J Agric Food Chem 2022 Aug 8;70(32):10031-10043. Epub 2022 Aug 8.

College of Food Science and Engineering, Jilin University, Changchun 130062, China.

Furan, a processing-induced food contaminant, has attracted great attention due to its hepatotoxicity. To further investigate the underlying mechanism of salidroside (SAL) alleviating furan-induced liver damage, we divided Balb/c mice into the control group, the furan (8 mg/kg/day) group, and three groups of three different doses of SAL (10/20/40 mg/kg/day) in the current research. The shifted serum profile was observed through untargeted metabonomics, to which the bile acid metabolism was related, and the alleviating effect of SAL against furan-induced apoptosis was caused by the metabolism. Target bile acid quantification for the liver and serum showed that SAL positively regulated the homeostasis of bile acids disturbed by furan. Meanwhile, SAL significantly upregulated the synthesis genes of bile acids (, , , and ) and the uptake transport genes ( and ) and downregulated the efflux transport genes (, , , , and 4). Transmission electron microscopy of the bile canaliculi and tight junctions and the levels of tight junction marker proteins (ZO-1, occludin, and claudin-1) confirmed that the disruption of the hepatic tight junction was inhibited by SAL. The connection between the apoptosis- and tight junction-related proteins was observed through the construction of a protein-protein interaction network. SAL suppressed the furan-induced hepatocyte apoptosis evidenced by the detection of TUNEL and Bax, Bcl-2, and caspase-3 levels. Taken together, SAL alleviated furan-induced hepatocyte apoptosis via altering the disordered homeostasis of bile acids and hepatic tight junctions.
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http://dx.doi.org/10.1021/acs.jafc.2c04654DOI Listing
August 2022

Isolation, genomic characterization, and mushroom growth-promoting effect of the first fungus-derived .

Front Microbiol 2022 22;13:947687. Epub 2022 Jul 22.

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

is a well-known edible and medicinal mushroom, and some bacteria isolated from mushroom sclerotia may have beneficial effects on their host. These mushroom growth-promoting bacteria (MGPBs) are of great significance in the mushroom production. In this work, we aimed to isolate and identify MGPBs from sclerotia. Using the agar plate dilution method, strain CACMS001 was isolated from sclerotia. The genome of CACMS001 was sequenced using PacBio platform, and the phylogenomic analysis indicated that CACMS001 could not be assigned to known species. In co-culture experiments, CACMS001 increased the mycelial growth of and and increased xylanase activity in . Comparative genomic analysis showed that CACMS001 lost almost all nitrogen fixation genes but specially acquired one redox cofactor cluster with , , , and involved in the synthesis of pyrroloquinoline quinone, a peptide-derived redox participating in phosphate solubilization activity. Strain CACMS001 has the capacity to solubilize phosphate using Pikovskaya medium, and and involved in this process in CACMS001 were revealed by quantitative real-time PCR. CACMS001 is a new potential species and is the first identified MGPB belonging to This novel bacterium would play a vital part in , , and other mushroom cultivation.
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http://dx.doi.org/10.3389/fmicb.2022.947687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354803PMC
July 2022

Identification and profiling of microRNAs involved in the regenerative involution of mammary gland.

Genomics 2022 Aug 3;114(5):110442. Epub 2022 Aug 3.

Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310015, PR China; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, PR China. Electronic address:

Regenerative involution is important for the subsequent lactation, but molecular mechanism has not been revealed. The crucial miRNA in tissue development indicates that miRNAs might participate in regenerative involution. In the present study, the mammary tissues of the dairy goats (n = 3) were collected via biopsy at wk-8 (time to dry off), -6, -4, -1, and + 1 relative to lambing for the Hematoxylin and Eosin staining and miRNA sequencing. Alveolar structures collapsed during regenerative involution, but the structures remained intact and distended. Among the 50 miRNA expression trajectories categorized by short time-series expression miner, two significant patterns were clustered. The differentially expressed miRNAs in the two patterns were mainly related to the self-renewal of tissue and enriched in pathways containing vesical-mediated transport, tissue development, tube development, vasculature development and epithelial development. The identification of the miRNAs will help in elucidating their regulatory roles in mammary gland development.
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http://dx.doi.org/10.1016/j.ygeno.2022.110442DOI Listing
August 2022

A Mycorrhizal Bacteria Strain Isolated From Exhibits Broad-Spectrum Antifungal Activity.

Front Plant Sci 2022 18;13:954160. Epub 2022 Jul 18.

State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

The microbes in the rhizosphere (or mycorrhizosphere) could promote plant growth, however, it is unclear whether mycorrhizosphere microbes could fight multiple fungal pathogens. In this study, twenty-one bacterial strains distributed in 6 genera, including 5 strains, were isolated from mycorrhizal samples of that rely on other fungi during their life cycles. Further screening and pot experiments showed that the strain ZL8 not only inhibited the growth of phytopathogenic fungi, but also promoted the growth of through inhibiting its wilting. In addition, strain ZL8 was found to have the ability to dissolve phosphate, produce IAA and siderophore. Nineteen compounds were identified from the fermentation broth of strain ZL8, of which 2,4-diacetylphloroglucinol (DAPG) had a significant inhibitory effect on phytopathogenic fungi with a minimum inhibitory concentration of 3.12-25 μg/mL. Molecular docking predicted that DAPG could bind to myosin I at two unique sites, which may be responsible to the inhibition of fungal growth. The evaluation results showed that strain ZL8 can be used to develop a dual-purpose biocontrol agents and biofertilizer. These results also provide new insights into the discovery and utilization of new resources for biocontrol agents and biolfertilizers.
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http://dx.doi.org/10.3389/fpls.2022.954160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340266PMC
July 2022

Relationship between Yes-Associated Protein 1 and Prognosis of Digestive System Neoplasm: Quantitative Analysis and Bioinformatics Analysis Based on 4023 Patients.

Evid Based Complement Alternat Med 2022 19;2022:3798694. Epub 2022 Jul 19.

Department of Gastroenterology, Daping Hospital, Army Medical University, Chongqing 400042, China.

Yes-associated protein 1 (YAP1) is involved in the development of a variety of malignancies. However, the prognosis of malignant digestive tumors with YAP1 expression is still controversial. This study searched 31 articles with 36 data sets of 4023 patients to explore the role of YAP1 expression on the prognosis of digestive malignant tumors by searching the PubMed, Embase, Web of Science, Google Scholar, and Cochrane Library databases. Specifically, relevant cancer expression matrix data were downloaded from The Cancer Genome Atlas (TCGA) database. In this meta-analysis, quantitative analysis showed that the overexpression of YAP1 was not conducive to OS (1.62, 95% CI (1.38, 1.90), =0.001) and DFS (1.59, 95% CI (1.31, 1.93), =0.001) in patients with digestive malignant tumors. In addition, TCGA database analysis showed that YAP1 was overexpressed in gastric cancer, cholangiocarcinoma, and colorectal cancer. Survival analysis showed that the patients with high expression of YAP1 in pancreatic cancer have a poor OS (MST: 394 vs. 691 days, < 0.0001) and DFS (MST: 371 vs. 542 days, =0.026) prognosis. YAP1 may be a molecular marker that effectively predicts the survival of malignant digestive tumors, especially pancreatic cancer, and is a potential therapeutic target for malignant digestive tumors.
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http://dx.doi.org/10.1155/2022/3798694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325623PMC
July 2022

Intelligent pH-sensing film based on polyvinyl alcohol/cellulose nanocrystal with purple cabbage anthocyanins for visually monitoring shrimp freshness.

Int J Biol Macromol 2022 Jul 27;218:900-908. Epub 2022 Jul 27.

Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524001, China.

We aimed to prepare a new pH-sensing film based on the immobilization of purple cabbage anthocyanins (PCA) into Polyvinyl alcohol (PVA) reinforced by cellulose nanocrystals (CNC). FT-IR, XRD and TGA were used to assess the intermolecular interactions and thermo-stability of films. The addition of CNC and PCA resulted in an enhancement in UV-vis barrier, mechanical properties and moisture resistance. Inclusion of PCA imparted intelligent properties to the films. PCA-loaded films displayed strong visually detectable colorimetric responses to pH (2-13) and volatile ammonia. When applied to monitor shrimp freshness at 4 °C, PVA/CNC films containing 0.6 % PCA exhibited conspicuous color fluctuations from purple to gray blue upon deterioration. As a result, PVA/CNC-PCA colorimetric films were considered as intelligent packaging labels with significant mechanical, water vapor barrier properties and pH-sensing qualities for visual quality evaluation of fresh seafood products.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.194DOI Listing
July 2022

Cooling Field Dependence of Exchange Bias in Mn-Doped Metal- Organic Framework [NH(CH)][FeFe(HCOO)].

J Phys Chem Lett 2022 Aug 29;13(31):7185-7190. Epub 2022 Jul 29.

National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing210093, People's Republic of China.

Herein, an intriguing exchange bias (EB) effect manifesting itself from positive to negative with an increase in the cooling field () is reported in the single crystal of Mn-doped metal-organic framework (MOF) [NH(CH)][FeFe(HCOO)] () by finely tuning the exchange interactions between the magnetic ions. Note that the doping ratio of Mn relative to the total metal ions is about 15%. Negative magnetization and EB below the compensation temperature were both observed in , and the EB field () changes its sign from positive to negative when is larger than ∼10 kOe. The abnormal dependence of EB can be interpreted explicitly by a combination of negative magnetization and couplings among the ions of Fe, Fe, and Mn with varying the . This work demonstrates a tunable EB in MOFs, in favor of designing novel magnetic devices.
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http://dx.doi.org/10.1021/acs.jpclett.2c01844DOI Listing
August 2022

An Empirical Study on Imagery and Emotional Response in Chinese Poetry Translation-The Visual Grammar Perspective.

Front Psychol 2022 12;13:872497. Epub 2022 Jul 12.

Faculty of Foreign Languages, Ningbo University, Ningbo, China.

The study investigated the evocation of mental imagery and emotional responses when English-Chinese bilinguals read classic Chinese poems and their English translations to examine (1) the target readers' formation of non-verbal text representations of Chinese poetry and (2) whether different translations affect the target readers' imagery cognition. A total of 20 English-Chinese speaker students enrolled in a Chinese university read a classic Chinese poem in Chinese and its four versions of translation in English. Through questionnaires and interviews, participants rated the visualized words used in the poems for the degree of mental imagery and emotional response evoked based on three indicators of narrative process, salience value, and emotive validity in the theoretical framework of visual grammar. Results showed considerable individual variances in the cognitive differences in forming mental imagery in all versions of the poems and there were also effects of translation strategy. Moreover, visual language information in poetry reading and its translations evoked different emotional responses depending on the use of visual words with cultural features. Our study demonstrates the applicability and accessibility of visual language in describing different readers' mental imagery and the interrelation and interaction between the poetry language system and the emotional, social, and cultural contexts involved in poetry translation.
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http://dx.doi.org/10.3389/fpsyg.2022.872497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321637PMC
July 2022

Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.).

Sci Rep 2022 Jul 28;12(1):12913. Epub 2022 Jul 28.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

Quantitative real-time polymerase chain reaction (qRT-PCR) is a sensitive and widely used technique for quantifying gene expression levels, and its accuracy depends on the reference genes used for data normalization. To date, no reference gene has been reported in the nutritious and functional vegetable okra (Abelmoschus esculentus L.). Herein, 11 candidates of reference genes were selected and evaluated for their expression stability in okra in different tissues at different developmental stages by using three software algorithms (geNorm, NormFinder, BestKeeper) and a web-based tool (RefFinder). Among them, eukaryotic initiation factor 4 alpha (eIF4A) and protein phosphatase 2A (PP2A) showed the highest stability, while TUA5 had the lowest stability. The combined usage of these two most stable reference genes was sufficient to normalize gene expression in okra. Then, the above results were further validated by normalizing the expression of the cellulose synthase gene CesA4. This work provides appropriate reference genes for transcript normalization in okra, which will facilitate subsequent functional gene research on this vegetable crop.
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http://dx.doi.org/10.1038/s41598-022-16124-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334609PMC
July 2022

Upregulated lncRNA-NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis.

Immun Inflamm Dis 2022 Aug;10(8):e627

Department of the Health and Nursing, Shanghai Nanhu Vocational and Technical College, Shanghai, China.

Introduction: Osteoporosis is related to lncRNA-neighboring enhancer of FOXA2 (NEF) and inversely correlated to ankylosing spondylitis (AS), implying that lncRNA-NEF might also relate to AS. Thus, the study was carried out to investigate the involvement of lncRNA-NEF in AS.

Methods: The study included 60 AS patients and 60 healthy controls. LncRNA-NEF expression in synovial fluid samples was analyzed by reverse transcription quantitative real-time polymerase chain reaction. Disease activity of the 60 AS patients was determined using the Ankylosing Spondylitis Disease Activity Score (ASDAS) 1-4 and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Western blot was carried out to investigate the effects of lncRNA-NEF on inflammatory factors in human fibroblast-like synovial (HFLS) cells. A 3-year follow-up was performed to analyze the role of lncRNA-NEF in the prediction of the recurrence of AS.

Results: Our study observed that lncRNA-NEF expression was upregulated in synovial fluid of AS patients and significantly correlated with the ASDAS 1-4, BASDAI, erythrocyte sedimentation rate (ESR), and C-reactive protein level (p < .05). Treatment with nonsteroidal anti-inflammatory drugs significantly downregulated lncRNA-NEF expression (p < .01). A 3-year follow-up showed that patients with high lncRNA-NEF levels had a high recurrence rate (hazard ratio = 2.266). In addition, lncRNA-NEF was found to regulate the expression of inflammatory factors in HFLS cells.

Conclusions: Therefore, lncRNA-NEF upregulation can predict recurrence and poor treatment outcomes of AS and has a great potential to serve as a predictive biomarker factor for the recurrent AS.
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http://dx.doi.org/10.1002/iid3.627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274798PMC
August 2022

Subchronic Toxicity of GmDREB3 Gene Modified Wheat in the Third Generation Wistar Rats.

Plants (Basel) 2022 Jul 12;11(14). Epub 2022 Jul 12.

Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China.

The aim of the current study was to evaluate the subchronic toxicity of GmDREB3 gene modified wheat in the third generation rats. SPF Wistar rats were fed with transgenic wheat diet (Gm), parental wheat diet (Jimai22) and AIN-93 rodent diet (Control), respectively, for two generations, to produce the third generation rats which were used for this study. The selected fresh weaned offspring rats (20/sex/group) were given the same diet as their parents for 13 weeks. No toxicity-related changes were observed in rats fed with Gm diet in the following respects: clinical signs, body weights, body weight gains, food consumption, food utilization rate, urinalysis, hematology, serum biochemistry and histopathology. The results from the present study demonstrated that 13 weeks consumption of Gm wheat did not cause any adverse effects in the third generation rats when compared with the corresponding Jimai22 wheat.
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http://dx.doi.org/10.3390/plants11141823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323929PMC
July 2022

Valuation of Ecosystem Services for the Sustainable Development of Hani Terraces: A Rice-Fish-Duck Integrated Farming Model.

Int J Environ Res Public Health 2022 Jul 13;19(14). Epub 2022 Jul 13.

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.

As a complementary and symbiotic agro-ecological cycle system, a nature-based integrated rice-fish-duck farming ecosystem was developed in the Honghe Hani Rice Terraces. The main research objective was to evaluate the ecosystem services based on case studies of the Hani integrated rice-fish-duck terraced farming system and determine its potential and its importance as an ecological asset. We developed a valuation model to assess the value of the integrated farming system based on the three aspects of provisioning, regulation and maintenance, and cultural services; we selected eight groups and 10 indictors to evaluate the ecosystem services of the integrated ecosystem in Honghe Hani Rice Terraces was 3.316 billion CNY, of which the provisioning service value was 1.76 billion CNY, the regulation and maintenance service value was 1.32 billion CNY, and the cultural services value was 230.85 million CNY. The evaluation will be useful as a theoretical reference for poverty alleviation policy makers in similar poverty-stricken areas, enabling them to better protect and promote this mode of farming and further promote the protection of the natural environment and cultural heritage alongside the sustainable development of natural resources and human well-being.
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http://dx.doi.org/10.3390/ijerph19148549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317269PMC
July 2022

Mxenes-Au NP Hybrid Plasmonic 2D Microplates in Microfluidics for SERS Detection.

Biosensors (Basel) 2022 Jul 10;12(7). Epub 2022 Jul 10.

Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.

Combined with microfluidics, surface-enhanced Raman spectroscopy (SERS) exhibits huge application prospective in sensitive online detection. In current studies, the design and optimization of plasmonic enhanced structures in microfluidics for SERS detection could be an interesting challenge. In this work, hybrid plasmonic 2D microplates composed of Mxenes (TiCT) microplates and in-situ synthesized Au nanoparticles (Au NPs) are fabricated in a microchannel for enhanced structures in SERS microfluidics. Benefiting from the 2D Mxenes microplates with complex distributions, the enhanced areas generated by Au NPs are quite enlarged in a microchannel, which exhibits high sensitivity in SERS detection at 10 M for Nile blue (NB) molecules in microfluidics. The mechanism of electromagnetic enhancement (EM) and chemical enhancement (CM) is analyzed. The experimental data indicate the ultrasonic times of Mxenes and the concentration of Au play important roles in the sensitivity of SERS detection, which is confirmed by the simulated electric field distributions. Furthermore, a typical pesticide (thiram) at 100 ppm in water is detected on these SERS microfluidics with hybrid plasmonic enhanced structures, which demonstrates that our work not only strengthens the knowledge of plasmonics but also enlarges the application of SERS.
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http://dx.doi.org/10.3390/bios12070505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312844PMC
July 2022

Exploration of Risk Factors for Poor Prognosis of Non-Traumatic Non-Aneurysmal Subarachnoid Hemorrhage.

Biomolecules 2022 07 6;12(7). Epub 2022 Jul 6.

Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

Background: Subarachnoid hemorrhage (SAH) is a devastating neurological disease associated with high rates of mortality and disability. Aneurysms are the main cause of non-traumatic subarachnoid hemorrhages. However, non-traumatic non-aneurysmal subarachnoid hemorrhage (naSAH), another clinical type of SAH, has been poorly studied for its prognosis and risk factors.

Method And Result: We collected demographic and clinical variables for 126 naSAH and 89 aneurysmal subarachnoid hemorrhage (aSAH) patients, including age and gender; hospitalization days; hematological indicators; clinical score scales; past medical history; and personal history. We found that the monocytes in naSAH (0.50 ± 0.26) patients were lower than in aSAH patients (0.60 ± 0.27). The prevalence of diabetes in naSAH (30.2%) patients was higher than in aSAH (14.5%) patients. The naSAH patients were divided into good and poor outcome groups based on the modified Rankin Scale at the 90th day (90-day mRS) after discharge. A univariate analysis showed that there were significant differences in age, white blood cell count (WBC), monocyte count, D-dipolymer, neuron-specific enolase (NSE), random blood glucose (RBG), aspartate transaminase (AST), urea and free triiodothyronine (FT3) between the two groups. A logistic regression showed that aging and high level NSE were independent risk factors for a poor outcome. The predictive ability of age (area under curve (AUC) = 0.71) and NSE (AUC = 0.68) were analyzed by a receiver operating characteristic (ROC) curve. The results of the logistic regression suggested that age, D-dipolymer, NSE, RBG, urea and FT3 distinguished and predicted the prognosis of naSAH. The discriminant analysis of the above variables revealed that the discriminant accuracy was 80.20%.

Conclusions: Compared with aSAHs, naSAHs are more likely to occur in patients with diabetes, and the level of monocytes is lower. Moreover, the prognosis of elderly patients with an naSAH is relatively poor, and the level of NSE in the course of the disease also reflects the prognosis. Multivariate comprehensive analysis is helpful to judge the prognosis of patients at a small cost.
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http://dx.doi.org/10.3390/biom12070948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313218PMC
July 2022

The Hepatoprotective and Hepatotoxic Roles of Sex and Sex-Related Hormones.

Front Immunol 2022 4;13:939631. Epub 2022 Jul 4.

Clinical Medicine Research Institute, Department of Metabolic and Bariatric Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.

Most liver diseases, including acute liver injury, drug-induced liver injury, viral hepatitis, metabolic liver diseases, and end-stage liver diseases, are strongly linked with hormonal influences. Thus, delineating the clinical manifestation and underlying mechanisms of the "sexual dimorphism" is critical for providing hints for the prevention, management, and treatment of those diseases. Whether the sex hormones (androgen, estrogen, and progesterone) and sex-related hormones (gonadotrophin-releasing hormone, luteinizing hormone, follicle-stimulating hormone, and prolactin) play protective or toxic roles in the liver depends on the biological sex, disease stage, precipitating factor, and even the psychiatric status. Lifestyle factors, such as obesity, alcohol drinking, and smoking, also drastically affect the involving mechanisms of those hormones in liver diseases. Hormones deliver their hepatic regulatory signals primarily classical and non-classical receptors in different liver cell types. Exogenous sex/sex-related hormone therapy may serve as a novel strategy for metabolic liver disease, cirrhosis, and liver cancer. However, the undesired hormone-induced liver injury should be carefully studied in pre-clinical models and monitored in clinical applications. This issue is particularly important for menopause females with hormone replacement therapy (HRT) and transgender populations who want to receive gender-affirming hormone therapy (GAHT). In conclusion, basic and clinical studies are warranted to depict the detailed hepatoprotective and hepatotoxic mechanisms of sex/sex-related hormones in liver disease. Prolactin holds a promising perspective in treating metabolic and advanced liver diseases.
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http://dx.doi.org/10.3389/fimmu.2022.939631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289199PMC
July 2022

Genomic and epigenomic alterations predict adaptive resistance and response to platinum-based therapy in patients with triple-negative breast and ovarian carcinomas.

Sci Transl Med 2022 Jul 6;14(652):eabn1926. Epub 2022 Jul 6.

Jackson Laboratory for Genomic Medicine, Farmington, CT 06030, USA.

Triple-negative breast cancer (TNBC) and ovarian carcinomas (OvCas) with promoter methylation (meth) respond more poorly to alkylating agents compared to those bearing mutations in and (mut). This is a conundrum given the biologically equivalent homologous recombination deficiency (HRD) induced by these genetic and epigenetic perturbations. We dissected this problem through detailed genomic analyses of TNBC and OvCa cohorts and experimentation with patient-derived xenografts and genetically engineered cell lines. We found that despite identical downstream genomic mutational signatures associated with meth and mut states, meth uniformly associates with poor outcomes. Exposure of meth TNBCs to platinum chemotherapy, either as clinical treatment of a patient or as experimental in vivo exposure of preclinical patient derived xenografts, resulted in allelic loss of methylation and increased expression and platinum resistance. These data suggest that, unlike mut cancers, where loss is a genetically "fixed" deficiency state, meth cancers are highly adaptive to genotoxin exposure and, through reversal of promoter methylation, recover expression and become resistant to therapy. We further found a specific augmented immune transcriptional signal associated with enhanced response to platinum chemotherapy but only in patients with BRCA-proficient cancers. We showed how integrating both this cancer immune signature and the presence of mutations results in more accurate predictions of patient response when compared to either HRD status or status alone. This underscores the importance of defining heterogeneity in optimizing the predictive precision of assigning response probabilities in TNBC and OvCa.
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http://dx.doi.org/10.1126/scitranslmed.abn1926DOI Listing
July 2022

Cognitive Heterogeneous Wireless Network and Artificial Intelligence-Based Supply Chain Efficiency Optimization Application.

Authors:
Yuan Yuan

Comput Intell Neurosci 2022 8;2022:8482365. Epub 2022 Jul 8.

Hebei University of Technology, Tianjin 300401, China.

Cognitive radio can specifically perceive the surrounding environment, can understand and learn the changes in the surrounding environment, and can adjust its own adaptation method, channel calculation, and other transmission parameters at any time. This cognitive radio method is more flexible and is an intelligent wireless communication system, a relatively advanced system. Cognitive communication devices can detect changes in the surrounding wireless environment and relatively change their own parameters to cope with the changes in the environment and ensure the stability of communication quality. As a different part of the supply chain, enterprises use the sharing economy and cooperation to improve the overall competitiveness of the enterprises in the supply chain, ultimately achieve a win-win effect for all enterprises in the supply chain, and complete the improvement of the company's competitiveness. The supply chain model can help companies to be familiar with the structure, operation process, and orientation of the supply chain in which they are located, so that they can clearly understand the work process through simulation scenarios and better understand the problems of the supply chain work process. Supply chain supply with cognitive heterogeneous wireless networks and artificial intelligence is a necessary way to deeply improve the operation and working process of supply chain control. In short, the cognitive radio system proposed in this study can receive network-related messages by observing the surrounding wireless network environment, and can receive internal communication tasks and emergency communication tasks based on historical data and prediction of future relationships. Through the access control process, we make an appropriate strategy and finally complete the communication function of the system.
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http://dx.doi.org/10.1155/2022/8482365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286962PMC
July 2022
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