Publications by authors named "Yuan Yu"

564 Publications

Inhibition of desmoglein-1 by aspirin leads to synthetic lethality of keratinocytes in Shuanghuanglian-induced cutaneous eruption response.

Toxicol Lett 2021 Jun 11. Epub 2021 Jun 11.

Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. Electronic address:

Cutaneous eruptions caused by the combination of aspirin (ASA) and Shuanghuanglian preparations have attracted widespread attention; however, the underlying mechanism remains unclear. This study aimed to evaluate the potential mechanism of cutaneous eruptions caused by a combination of Shuanghuanglian injection powder (SHL) and ASA in vivo and in vitro. ASA and SHL co-administration induced inflammatory responses in HaCat cells, as evidenced by marked increases in the expression of IL-4 and TNF-α, and the level of apoptosis. Additionally, histopathological investigation of mice skin tissues showed local inflammatory cell infiltration. Western boltting was used to detect the effects of ASA on desmoglein-1 (DSG1) expression; we found that DSG1 expression was down-regulated in vivo and in vitro. Finally, the key components of SHL were administered to HaCat cells with down-regulated DSG1; it was seen that neochlorogenic acid and rutin have a significant effect on HaCat cell apoptosis. These results demonstrate that DSG1 deficiency is a potential cause of cutaneous eruptions caused by the combination of SHL and ASA, and neochlorogenic acid and rutin are the main allergenic components. This study provides a new research strategy for the safety evaluation of integrated traditional Chinese and Western medicine.
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http://dx.doi.org/10.1016/j.toxlet.2021.06.005DOI Listing
June 2021

Dynamics of the SARS-CoV-2 antibody response up to 10 months after infection.

Cell Mol Immunol 2021 Jun 7. Epub 2021 Jun 7.

Ministry of Education and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1038/s41423-021-00708-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182358PMC
June 2021

Constructing anatase TiO/Amorphous NbO heterostructures to enhance photocatalytic degradation of acetaminophen and nitrogen oxide.

J Colloid Interface Sci 2021 May 25;601:346-354. Epub 2021 May 25.

Department of Chemistry, Faculty of Science, King Khalid University, Abha, 61413, Saudi Arabia; Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia.

TiO nanostructures have been one of the most explored metal oxides photocatalysts to apply for environmental remediation. However, its wide band gap results in the underutilization of sunlight for degradation of pollutants. In order to overcome this handicap, the synthesis of TiO-based composite has brought extraordinary materials. In this study, we design and prepare TiO/NbO heterostructures with different molar ratios by using peroxotitanium and peroxoniobium complex as precursors in aqueous solution. The TiO exists in the form of anatase while NbO is amorphous in the composite, leading to a special crystalline TiO/amorphous NbO heterostructures. In particular, Nb element is also doped and Ti ions are formed in the TiO lattice, leading to a reduced band gap. The unique TiO/0.25NbO (Ti:Nb = 2:1) heterostructures can effectively suppress the recombination of photogenerated electrons and holes, and facilitate the charge transfer, resulting in the optimum photocatalytic performance. The nitrogen oxide removal efficiency by TiO/0.25NbO is 77.23% in visible light, which is 3.8-folds and 7.0-folds higher than pure TiO and NbO Photocatalytic degradation of acetaminophen by TiO/0.25NbO is 90.6% in visible light, which is approximately 2.5-folds higher than pure TiO and NbO.
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http://dx.doi.org/10.1016/j.jcis.2021.05.120DOI Listing
May 2021

N-Heterocyclic Carbene-Stabilized Gold Nanoclusters in a Metal-Organic Framework for Photocatalytic CO2 Reduction.

Angew Chem Int Ed Engl 2021 Jun 1. Epub 2021 Jun 1.

Tongji University, Department of Chemistry, 1239 Siping Rd., 200092, Shanghai, CHINA.

Ultrafine gold nanoclusters (Au-NCs) are susceptible to migrate and aggregate, even in porosity of many crystalline solids.  N-heterocyclic carbenes (NHCs) are a class of structurally diverse ligands for stabilization of Au-NCs in homogeneous chemistry, showing catalytic reactivity in CO 2 activation. Herein, for the first time, we demonstrate a heterogeneous nucleation approach to stabilize ultrasmall and highly dispersed gold nanoclusters in a metal-free NHC-functionalized porous matrix. The sizes of gold nanoclusters are tunable from 1.3 nm to 1.8 nm based on the interpenetration of metal-organic framework (MOF) topology. Control experiments using amine or imidazolium-functionalized MOFs afforded aggregation of Au species. The resultant [email protected] composite exhibits steady and excellent activity in photocatalytic CO 2 reduction, superior to the control mixtures without NHC-ligand stabilization.  The mechanistic studies reveal the synergistic catalytic effect of MOFs and Au-NCs through the MOF-NHC-Au covalent bonding bridges.
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http://dx.doi.org/10.1002/anie.202105420DOI Listing
June 2021

-Mediated Active Responses of Plant Growth under Salt and Temperature Stress in .

Plants (Basel) 2021 May 31;10(6). Epub 2021 May 31.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

The transcription factor NF-YB (nuclear factor-YB) family is a subfamily of the nuclear factor Y (NF-Y), which plays an important role in regulating plant growth, development and participates in various stress responses. Although the NF-Y family has been studied in many species, it is still obscure in . In this study, 23 genes in eucalyptus were identified and unevenly distributed on 11 chromosomes. Phylogenetic analysis showed the genes were divided into two clades, LEC-1 type and non-LEC1 type. The evolution of distinct clades was relatively conservative, the gene structures were analogous, and the differences of genetic structures among clades were small. The expression profiles showed that the distinct genes were highly expressed in diverse tissues, and functioned in response to salinity, heat and cold stresses. Our study characterized the phylogenetic relationship, gene structures and expression patterns of gene family and investigated their potential roles in abiotic stress responses, which provides solid foundations for further functional analysis of genes in eucalyptus.
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http://dx.doi.org/10.3390/plants10061107DOI Listing
May 2021

B and cyano groups co-doped g-CN with multiple defects for photocatalytic nitrogen fixation in ultrapure water without hole scavengers.

J Colloid Interface Sci 2021 May 18;600:639-648. Epub 2021 May 18.

Materials Institute of Atomic and Molecular Science, Shaanxi University of Science and Technology, Xi'an 710021, China.

B atoms and cyano groups co-doped graphite carbon nitride with nitrogen vacancies (VN-BC-CN) was explored via one-step in-situ route. A series of comprehensive experiments confirmed that B atoms and cyano groups had been doped into the framework of graphite carbon nitride, forming VN-BC-CN catalyst sample with a large number of nitrogen-vacancy defects. As electron acceptors, B and cyano groups could be used as active sites for nitrogen conversion. The defect level caused by nitrogen vacancy led to red shift of the light absorption edge, which resulted in higher separation efficiency of photo-induced carriers and faster transfer rate of photo-induced electrons for the VN-BC-CN catalyst. This VN-BC-CN catalyst had good photocatalytic nitrogen fixation performance in the ultrapure water without any hole-scavengers. The nitrogen photofixation rate of VN-BC-CN (115.53 μmol g h) was 25.5 times that of pure carbon nitride (GCN, 4.53 μmol g h). Moreover, NH generation rate hardly decreased after 10 h reaction, and the NH generation rate could reach 79.56 μmol g h in the fifth cycle, showing the good photocatalytic stability of the VN-BC-CN catalyst.
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http://dx.doi.org/10.1016/j.jcis.2021.05.075DOI Listing
May 2021

A cyclic nano-reactor achieving enhanced photodynamic tumor therapy by reversing multiple resistances.

J Nanobiotechnology 2021 May 21;19(1):149. Epub 2021 May 21.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan, China.

Background: Photodynamic therapy (PDT) is a clinically implemented modality to combat malignant tumor, while its efficacy is largely limited by several resistance factors from tumor microenvironment (TME), such as hypoxia, anti-oxidant systems, and ATP-dependent tumor adaptive resistances. The aim of this work is to construct a multifunctional nanoplatform to remodel multiple resistant TME for enhanced PDT.

Results: Here, a targeting nano-reactor was facilely constructed to reverse the multiple resistances of PDT by incorporating glucose oxidase (GOx) and chlorin e6 (Ce6) into poly (D, L-lactic-co-glycolic acid) (PLGA)/ metal-organic framework (MOF) core-shell nanoassembly, with surface deposition of hyaluronic acid (HA) stabilized MnO. The nano-reactor could selectively target tumor cells by virtue of surface HA modification, and once internalization, a few reactions were initiated to modulate TME. Glucose was consumed by GOx to inhibit ATP generation, and the produced HO was catalyzed by MnO to generate O for tumor hypoxia alleviation and photodynamic sensitization, and glutathione (GSH) was also effectively depleted by MnO to suppress the tumor antioxidant defense. Consequently, the nano-reactor achieved robust PDT with amplified tumor therapy via intravenous injection.

Conclusions: This nano-reactor offers a multifunctional nanoplatform to sensitize TME-limited tumor treatment means via reversing multiple resistances.
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http://dx.doi.org/10.1186/s12951-021-00893-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139056PMC
May 2021

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

J Clin Lab Anal 2021 May 21:e23822. Epub 2021 May 21.

Wenzhou Key Laboratory of Birth Defects, Wenzhou Central Hospital, Dingli Clinical Medical College of Wenzhou Medical University, Wenzhou, China.

Background: QRFPR is a recently identified member of the G protein-coupled receptor and is an orphan receptor for 26Rfa, which plays important role in the regulation of many physiological functions.

Methods: Here, we employed whole exome sequencing (WES) to examine the patients with intellectual disability (ID) and difficulty in feeding. We performed SIFT and PolyPhen2 predictions for the variants. The structure model was built from scratch by I-TASSER. Here, results derived from a number of cell-based functional assays, including shRNA experiment, intracellular Ca measurement, the expression of PI3 K-AKT-mTOR, and phosphorylation. The functional effect of QRFPR variants on PI3K-AKT-mTOR signaling was evaluated in vitro transfection experiments.

Result: Here, we identified two QRFPR variants at c.202 T>C (p.Y68H) and c.1111C>T (p.R371W) in 2 unrelated individuals. Structural analysis revealed that p.Y68H and p.R371W variants may affect the side chain structure of adjacent amino acids causing reduced binding of QRFPR to 26Rfa. The results show that QRFPR stimulated by 26Rfa leading to the transient rise of intracellular Ca . The QRFPR variations p.Y68H and p.R371 W can reduce the mobilization of intracellular Ca . The phosphorylation levels of the PI3K, Akt, and mTOR were significantly up- or downregulated by QRFPR overexpression or silencing, respectively. The QRFPR variations inhibited PI3K-AKT-mTOR signaling, resulting in downregulation of p-mTOR.

Conclusions: Our findings suggest that QRFPR acts as important role in neurodevelopment, and the effects of QRFPR are likely to be mediated by the Ca -dependent PI3K-AKT-mTOR pathways. Importantly, these findings provide a foundation for future elucidation of GPCR-mediated signaling and the physiological implications.
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http://dx.doi.org/10.1002/jcla.23822DOI Listing
May 2021

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella.

Pest Manag Sci 2021 May 18. Epub 2021 May 18.

State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Backgroud: Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G-protein-coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests.

Results: In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5-HT , Px5-HT , Px5-HT , Px5-HT , and Px5-HT ). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2.

Conclusions: Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species.
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http://dx.doi.org/10.1002/ps.6481DOI Listing
May 2021

The effects of locomotion on bone marrow mesenchymal stem cell fate: insight into mechanical regulation and bone formation.

Cell Biosci 2021 May 17;11(1):88. Epub 2021 May 17.

School of Physical Education & Sports Science, South China Normal University, 55 Zhongshan Road West, Tianhe District, Guangzhou, 510631, Guangdong, China.

Bone marrow mesenchymal stem cells (BMSCs) refer to a heterogeneous population of cells with the capacity for self-renewal. BMSCs have multi-directional differentiation potential and can differentiate into chondrocytes, osteoblasts, and adipocytes under specific microenvironment or mechanical regulation. The activities of BMSCs are closely related to bone quality. Previous studies have shown that BMSCs and their lineage-differentiated progeny (for example, osteoblasts), and osteocytes are mechanosensitive in bone. Thus, a goal of this review is to discuss how these ubiquious signals arising from mechanical stimulation are perceived by BMSCs and then how the cells respond to them. Studies in recent years reported a significant effect of locomotion on the migration, proliferation and differentiation of BMSCs, thus, contributing to our bone mass. This regulation is realized by the various intersecting signaling pathways including RhoA/Rock, IFG, BMP and Wnt signalling. The mechanoresponse of BMSCs also provides guidance for maintaining bone health by taking appropriate exercises. This review will summarize the regulatory effects of locomotion/mechanical loading on BMSCs activities. Besides, a number of signalling pathways govern MSC fate towards osteogenic or adipocytic differentiation will be discussed. The understanding of mechanoresponse of BMSCs makes the foundation for translational medicine.
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http://dx.doi.org/10.1186/s13578-021-00601-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130302PMC
May 2021

Total IgE Variability Is Associated with Future Asthma Exacerbations: A 1-Year Prospective Cohort Study.

J Allergy Clin Immunol Pract 2021 May 12. Epub 2021 May 12.

Airway Physiology and Imaging Group and Woolcock Emphysema Centre, Woolcock Institute of Medical Research, Sydney, New South Wales, Australia; Discipline of Medical Sciences, School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.

Background: Few prospective studies have investigated the relationship between IgE variability and risk for asthma exacerbations (AEs).

Objective: To explore the relationship between IgE variability and AEs.

Methods: Recruited patients with stable asthma underwent two serum total IgE tests within a month (at screening [baseline IgE] and at 1 month) to obtain the coefficient of variation (CV) of base 10 log-transformed IgE. Patients with IgE CV were divided into IgE CV-high and IgE CV-low cohorts based on the CV median and were observed within 12 months, during which the association between IgE variability and AEs was explored using a negative binomial regression model.

Results: The IgE CV levels obtained from 340 patients classified patients into two groups (n = 170 for the IgE CV-high and IgE CV-low groups, respectively) based on the serum total IgE CV median of 2.12% (quartiles 1 and 3: 0.98% and 3.91%, respectively). The IgE CV-high patients exhibited worse asthma control and lung function and more marked airway inflammation, and received more intensive medication use compared with IgE CV-low patients. The IgE CV-high patients exhibited increased rates of moderate-to-severe (adjusted rate ratio = 2.88; 95% confidence interval, 1.65-5.03; P < .001) and severe (adjusted rate ratio = 2.16; 95% confidence interval, 1.08-4.32; P = .029) AEs during the follow-up year compared with IgE CV-low patients. Furthermore, sputum IL-6 partially mediated the associations between IgE CV with moderate-to-severe and severe AEs.

Conclusions: Variability in total serum IgE levels is an easily obtained and practical measure for predicting AEs. Future studies are needed to investigate whether IgE variability can be used to guide precision medicine in asthma.
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http://dx.doi.org/10.1016/j.jaip.2021.04.065DOI Listing
May 2021

Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway.

Vet Microbiol 2021 Jul 4;258:109098. Epub 2021 May 4.

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China. Electronic address:

Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection via immunosuppression. Although the co-infection of PCV2 and porcine parvovirus (PPV) is commonly observed in worldwide, the relative immune mechanisms promoting PPV infection in PCV2-infected piglets are currently unknown. Herein, we found that PCV2 infection suppressed IFN-β expression and promoted PPV infection in the piglets. Consistent with this finding, we confirmed that PCV2 infection significantly inhibited the induction of IFN-β to promote PPV replication in cell level. Furthermore, PCV2 infection attenuated the K63-linked ubiquitination of STING induced by PPV, blocked the formation of complex of STING, TBK1 and IRF3, and further prevented the phosphorylation of TBK1 and IRF3, resulting in a decreased IFN-β transcription response to PPV infection. Consistently, using cGAMP to direct stimulate STING also appeared a reduced STING-K63 ubiquitination and IFN-β induction in PCV2-infected cells. However, we noted that knockdown of p38-MAPK signaling could markedly attenuate the inhibitory effect of PCV2 on STING-K63 ubiquitination, and improve the induction of IFN-β in PCV2-infected whenever theses cells were challenged with PPV infection or cGAMP stimulation. Meanwhile, we found that PCV2 infection promoted the phosphorylation of USP21 to inhibit the K63 ubiquitination of STING and the transcription of IFN-β via activation of p38-MAPK signaling. Taken together, our results demonstrate that PCV2 infection activates the p38-MAPK signaling pathway-mediated USP21 phosphorylation to inhibit the K63 ubiquitination of STING, which prevents the phosphorylation and transportation to the nucleus of IRF3, leading to an increase risk for PPV infection.
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http://dx.doi.org/10.1016/j.vetmic.2021.109098DOI Listing
July 2021

Dual-triggered biomimetic vehicles enable treatment of glioblastoma through a cancer stem cell therapeutic strategy.

Nanoscale 2021 Apr 8;13(15):7202-7219. Epub 2021 Apr 8.

Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.

Glioma stem cells (GSCs) and their complex microenvironment play a crucial role in the high invasion of cancer and therapeutic resistance and are considered to be the most likely cause of cancer relapse. We constructed a biomimetic vehicle (LDL-SAL-Ang) based on a low density lipoprotein triggered by Angiopep-2 peptide and ApoB protein, to improve the transport of an anti-GSC therapeutic agent into the brain. The LDL-SAL-Ang showed significant inhabitation for GSC microsphere formation and induced the highest apoptotic rate in two types of GSCs. LDL-SAL-Ang reduced the number of GSC-derived endothelial tubules at a lower drug concentration and inhibited endothelial cell migration and angiogenesis. The pharmacokinetic analysis showed that the brain tissue uptake rate (% ID g) for LDL-SAL-Ang was significantly enhanced at 0.45. For anti-glioblastoma activity in vivo, the median survival time of LDL-SAL-Ang plus temozolomide group was 47 days, which were significantly increased compared with the control or temozolomide only groups. The endogenous biomimetic nanomedicine that we designed provides a potential approach to improve treatments for intracranial tumors and reduced neurotoxicity of nanomedicine.
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http://dx.doi.org/10.1039/d0nr08899dDOI Listing
April 2021

Bioinformatic analysis identifies potential biomarkers and therapeutic targets of septic-shock-associated acute kidney injury.

Hereditas 2021 Apr 16;158(1):13. Epub 2021 Apr 16.

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1277, Jiefang Avenue, Wuhan, 430022, China.

Background: Sepsis and septic shock are life-threatening diseases with high mortality rate in intensive care unit (ICU). Acute kidney injury (AKI) is a common complication of sepsis, and its occurrence is a poor prognostic sign to septic patients. We analyzed co-differentially expressed genes (co-DEGs) to explore relationships between septic shock and AKI and reveal potential biomarkers and therapeutic targets of septic-shock-associated AKI (SSAKI).

Methods: Two gene expression datasets (GSE30718 and GSE57065) were downloaded from the Gene Expression Omnibus (GEO). The GSE57065 dataset included 28 septic shock patients and 25 healthy volunteers and blood samples were collected within 0.5, 24 and 48 h after shock. Specimens of GSE30718 were collected from 26 patients with AKI and 11 control patents. AKI-DEGs and septic-shock-DEGs were identified using the two datasets. Subsequently, Gene Ontology (GO) functional analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis were performed to elucidate molecular mechanisms of DEGs. We also evaluated co-DEGs and corresponding predicted miRNAs involved in septic shock and AKI.

Results: We identified 62 DEGs in AKI specimens and 888, 870, and 717 DEGs in septic shock blood samples within 0.5, 24 and 48 h, respectively. The hub genes of EGF and OLFM4 may be involved in AKI and QPCT, CKAP4, PRKCQ, PLAC8, PRC1, BCL9L, ATP11B, KLHL2, LDLRAP1, NDUFAF1, IFIT2, CSF1R, HGF, NRN1, GZMB, and STAT4 may be associated with septic shock. Besides, co-DEGs of VMP1, SLPI, PTX3, TIMP1, OLFM4, LCN2, and S100A9 coupled with corresponding predicted miRNAs, especially miR-29b-3p, miR-152-3p, and miR-223-3p may be regarded as promising targets for the diagnosis and treatment of SSAKI in the future.

Conclusions: Septic shock and AKI are related and VMP1, SLPI, PTX3, TIMP1, OLFM4, LCN2, and S100A9 genes are significantly associated with novel biomarkers involved in the occurrence and development of SSAKI.
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http://dx.doi.org/10.1186/s41065-021-00176-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052759PMC
April 2021

Activity in projection neurons from prelimbic cortex to the PVT is necessary for retrieval of morphine withdrawal memory.

Cell Rep 2021 Apr;35(1):108958

State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai 200032, China; Department of Neurology of Zhongshan Hospital, MOE Frontier Center for Brain Science, School of Basic Medical Sciences, Shanghai, China. Electronic address:

Previous work has shown that the paraventricular nucleus of the thalamus (PVT) is an important region that is involved in the conditioned context-induced retrieval of morphine withdrawal memory. However, the upstream neural circuits that activate the PVT to participate in the conditioned context-induced retrieval of morphine withdrawal memory remain unknown. In the present work, we find that the conditioned context activates projection neurons from the prelimbic cortex (PrL) to the PVT, and the inhibition of PrL-PVT projection neurons inhibits the conditioned context-induced retrieval of morphine withdrawal memory; the conditioned context induces an increase in Arc expression, intrinsic excitability, and glutamate output in PrL-PVT projection neurons in morphine-withdrawn mice. These results suggest that the activity of PrL-PVT projection neurons is necessary for the retrieval of morphine withdrawal memory, and the conditioned context causes a plastic change in the activity in these projection neurons during the withdrawal memory retrieval.
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http://dx.doi.org/10.1016/j.celrep.2021.108958DOI Listing
April 2021

The clinical evaluation of preoperative abdominal aortic balloon occlusion for patients with placenta increta or percreta.

J Matern Fetal Neonatal Med 2021 Apr 1:1-6. Epub 2021 Apr 1.

Department of General Surgery, Chongqing Hechuan District Maternal and Child Health Care Hospital, Chongqing, China.

Objective: To evaluate the efficacy and safety of abdominal aortic balloon for pregnant women with placenta increta or percreta (PIP).

Methods: Retrospective analysis of the parameters containing estimated blood loss, red cell suspension (RCS) transfusion volume, hysterectomy, surgery time, postoperative hospital days, neonatal status and complications between the two groups.

Results: The patients with preoperative abdominal aortic balloon occlusion (AABO) had significant reduction in blood loss volume, red cell suspension transfusion volume and plasma transfusion volume compared to patients without balloon. Similarly, the surgery time and hysterectomy were obviously reduced in the AABO group. However, there were no difference in the Apgar scores and neonatal complications between the two groups, indicating that the abdominal aortic balloon has little adverse effect on the newborns.

Conclusion: AABO plays dramatic roles on reducing blood loss volume and blood transfusion volume and it is also a safe and effective technology providing new insight into the therapy of patient with PIP.

Synopsis: Preoperative abdominal aortic balloon occlusion (AABO), as a new intravascular interventional therapy, is safe and effective in patients with placenta increta or percreta.
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http://dx.doi.org/10.1080/14767058.2021.1906219DOI Listing
April 2021

Synchronous removal of emulsions and organic dye over palladium nanoparticles anchored cellulose-based membrane.

J Environ Manage 2021 Jun 25;288:112402. Epub 2021 Mar 25.

State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin, 150040, PR China. Electronic address:

Membrane is a considerable precursor for emulsions separation and organic dyes degradation used in water purification and oil reclamation. However, the tedious preparation method, the surface smears easily, and low degradation efficiency, these characteristics usually significantly hinder its applicability toward wastewater governance. Herein, a green, facile, and efficient fabrication strategy to prepare a bi-functional palladium nanoparticles (PdNPs)-loaded bacterial cellulose membrane (BCMPd) is proposed. A tri-functional bacterial cellulose membrane (BCM) was obtained by percolating bacterial cellulose (BC) on a basal membrane, and BCM served as a support, reducing agent, and stabilizer in the subsequent reduction of PdNPs. Bi-functional BCMPd was successfully obtained and used for continuously removing emulsions and reducing methylene blue (MB) from simulated wastewater via the integration of physical sieving and chemical reaction. Meanwhile, the enhancement factors for the water transfer ability and demulsification capacity correlated directly with the wettability and surface structure of BCMPd. Furthermore, the dosage of BC was adjusted to reveal the mechanism for the enhanced water transferability and demulsification capacity. Notably, PdNPs of BCMPd decreased Fermi potential difference between BH and MB, accelerating the electron transfer of the reduction reaction and thus exhibiting a remarkable MB degradation efficiency. Together, the information obtained in this work can be useful for comprehensively addressing the bottleneck of forming a cost-effective, eco-friendly, and bi-functional membrane reactor, providing an alternative approach for better treatment of complex wastewater.
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http://dx.doi.org/10.1016/j.jenvman.2021.112402DOI Listing
June 2021

Teaming with NAADP.

Sci Signal 2021 Mar 23;14(675). Epub 2021 Mar 23.

Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK.

NAADP is a potent Ca-mobilizing messenger linked to numerous Ca-dependent effects across the natural world. On 18 February 2021, delegates attended a virtual symposium to discuss studies of the mechanisms of action of NAADP, including the work of Gunaratne and Roggenkamp in this issue of .
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http://dx.doi.org/10.1126/scisignal.abh2798DOI Listing
March 2021

Ultrasound-Assisted Precise In Situ Decompression for Cubital Tunnel Syndrome.

Orthop Surg 2021 May 21;13(3):840-846. Epub 2021 Mar 21.

EMG Room, Tianjin Hospital, Tianjin, China.

Objective: To explore the effect of locating the ulnar nerve compression sites and guiding the small incision so as to decompress the ulnar nerve in situ on the elbow by high-frequency ultrasound before operation.

Methods: A retrospective analysis was conducted on 56 patients who underwent ultrasound-assisted in situ decompression for cubital tunnel syndrome from May 2018 to August 2019. The patients' average age was 51.13 ± 7.35 years, mean duration of symptoms was 6.51 ± 1.96 months, and mean postoperative follow-up was 6.07 ± 0.82 months. Nine patients had Dellon's stage mild, 39 had stage moderate, and eight had stage severe. Ultrasound and electromyography were completed in all patients before operation. The presence of ulnar nerve compressive lesion, the specific location, and the reason and extent of compression were determined by ultrasound. A small incision in situ surgery was given to decompress the ulnar nerve according to the pre-defined compressive sites.

Results: All patients underwent in situ decompression. The compression sites around the elbow were as follows: two in the arcade of Struthers, one in the medial intermuscular septum, four in the anconeus epitrochlearis muscle, five beside the cyst of the proximal flexor carpi ulnaris (FCU), and the remaining 44 cases were all from the compression between Osborne's ligament to the two heads of the FCU. The compression localizations diagnosed by ultrasound were confirmed by operations. Preoperative ultrasound confirmed no ulnar nerve subluxation in all cases. The postoperative outcomes were satisfactory. There was no recurrence or aggravation of symptoms in this group of patients according to the modified Bishop scoring system; results showed that 43 cases were excellent, 10 were good, and three were fair.

Conclusions: High-frequency ultrasound can accurately and comprehensively evaluate the ulnar nerve compression and the surrounding tissues, thus providing significant guidance for the precise minimally invasive treatment of ulnar nerve compression.
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http://dx.doi.org/10.1111/os.12922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126903PMC
May 2021

Detection of renal allograft fibrosis with MRI: arterial spin labeling outperforms reduced field-of-view IVIM.

Eur Radiol 2021 Mar 18. Epub 2021 Mar 18.

Department of Medical Imaging, Jinling Hospital, Clinical School of Southern Medical University, Nanjing, 210002, Jiangsu, China.

Objective: To compare the value of reduced field-of-view (FOV) intravoxel incoherent motion (IVIM) diffusion-weighted imaging (DWI) and arterial spin labeling (ASL) for assessing renal allograft fibrosis and predicting long-term dysfunction.

Methods: This prospective study included 175 renal transplant recipients undergoing reduced FOV IVIM DWI, ASL, and biopsies. Renal allograft fibrosis was categorized into ci0, ci1, ci2, and ci3 fibrosis according to biopsy results. A total of 83 participants followed for a median of 39 (IQR, 21-42) months were dichotomized into stable and impaired allograft function groups based on follow-up estimated glomerular filtration rate. Total apparent diffusion coefficient (ADC), pure diffusion ADC, pseudo-perfusion ADC, perfusion fraction f from IVIM DWI, and renal blood flow (RBF) from ASL were calculated and compared. The area under the receiver operating characteristic curve (AUC) was calculated to assess the diagnostic and predictive performances.

Results: RBF was different in ci0 vs ci1 (147.9 ± 46.3 vs 126.0 ± 49.4 ml/min/100 g, p = .02) and ci2 vs ci3 (92.9 ± 46.9 vs 70.8 ± 37.8 ml/min/100 g, p = .03). RBF in the stable group was higher than that in the impaired group (144.73 ± 49.33 vs 102.19 ± 47.58 ml/min/100 g, p < .001). AUCs in distinguishing renal allograft fibrosis and predicting long-term allograft dysfunction for RBF were higher than cortical ADC (ci0 vs ci1-3, 0.76 vs 0.59, p < .001; ci0-1 vs ci2-3, 0.79 vs 0.68, p = .01; ci0-2 vs ci3, 0.79 vs 0.68, p = .01; 0.76 vs 0.60, p = .04, respectively).

Conclusion: Compared to reduced FOV IVIM DWI, ASL was a more promising technique for noninvasively distinguishing renal allograft fibrosis degree and predicting long-term allograft dysfunction.

Key Points: • Compared to total ADC from rFOV IVIM DWI, RBF from ASL can distinguish no fibrosis (ci0) vs mild fibrosis (ci1) (p = .02) and moderate fibrosis (ci2) vs severe fibrosis (ci3) (p = .04). • RBF had superior performance than diffusion parameters in discriminating fibrosis (no fibrosis [ci0] vs fibrosis [ci1-3], mild fibrosis [ci0-1] vs moderate to severe fibrosis [ci2-3], non-severe [ci0-2] vs severe [ci3] fibrosis; AUC = 0.76 vs 0.59, p < .001; 0.79 vs 0.68, p = .01; 0.79 vs 0.68, p = .01). • Compared to reduced FOV IVIM DWI, ASL was a more promising technique for noninvasively predicting long-term allograft dysfunction (AUC = 0.76 vs 0.60, p = .04).
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http://dx.doi.org/10.1007/s00330-021-07818-9DOI Listing
March 2021

Author Correction: Metabolite signatures of diverse Camellia sinensis tea populations.

Nat Commun 2021 Mar 17;12(1):1829. Epub 2021 Mar 17.

FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.

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http://dx.doi.org/10.1038/s41467-021-22150-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969921PMC
March 2021

Past Shift Work and Incident Coronary Heart Disease in Retired Workers: A Prospective Cohort Study.

Am J Epidemiol 2021 Mar 17. Epub 2021 Mar 17.

Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Present shift work has been associated with coronary heart disease (CHD) among employed workers, but it remains unclear whether shift work in the past is still associated with CHD in retired workers. We recruited 21,802 retired workers in Shiyan, China in 2008-2010 and 2013, and followed them up for CHD events to December 31, 2018. Retired workers with longer duration of past shift work had higher CHD risks (hazard ratios for those with ≤5.0, 5.25 to 10.0, 10.5 to 20.0, and >20.0 years of past shift work were 1.05 (95% confidence interval: 0.94, 1.16), 1.08 (0.94, 1.25), 1.23 (1.07, 1.42), and 1.28 (1.08, 1.51)). The association was substantially higher among services or sales workers than among manufacturing or manual labor workers (hazard ratio for every 5-year increase in past shift work, 1.11 (95% confidence interval: 1.05, 1.16) versus 1.02 (0.98, 1.06)). Moreover, the risk was lower among those who were physically active than their inactive counterparts (P for interaction, 0.019). Longer duration of past shift work was associated with higher risk of incident CHD among retired workers, especially those from services or sales sectors. Physical exercise might be beneficial in reducing the excess risk.
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http://dx.doi.org/10.1093/aje/kwab074DOI Listing
March 2021

Prospective Study on Plasma MicroRNA-4286 and Incident Acute Coronary Syndrome.

J Am Heart Assoc 2021 Mar 10;10(6):e018999. Epub 2021 Mar 10.

Department of Occupational and Environmental Health Key Laboratory of Environment and Health Ministry of Education and State Key Laboratory of Environmental Health (Incubating) School of Public Health Tongji Medical CollegeHuazhong University of Science and Technology Wuhan China.

Background Mounting evidence suggests that circulating microRNAs (miRNAs) are critical indicators of cardiovascular disease. However, prospective studies linking circulating miRNAs to incident acute coronary syndrome (ACS) are limited, and the underlying effect of associated miRNA on incident ACS remains unknown. Methods and Results Based on a 2-stage prospective nested case-control design within the Dongfeng-Tongji cohort, we profiled plasma miRNAs from 23 pairs of incident ACS cases and controls by microarray and validated the candidate miRNAs in 572 incident ACS case-control pairs using quantitative real-time polymerase chain reaction. We observed that plasma miR-4286 was associated with higher risk of ACS (adjusted odds ratio according to an interquartile range increase, 1.26 [95% CI, 1.07-1.48]). Further association analysis revealed that triglyceride was positively associated with plasma miR-4286, and an interquartile range increase in triglyceride was associated with an 11.04% (95% CI, 3.77%-18.83%) increase in plasma miR-4286. In addition, the Mendelian randomization analysis suggested a potential causal effect of triglyceride on plasma miR-4286 ( coefficients: 0.27 [95% CI, 0.01-0.53] and 0.27 [95% CI, 0.07-0.47] separately by inverse variance-weighted and Mendelian randomization-pleiotropy residual sum and outlier tests). Moreover, the causal mediation analysis indicated that plasma miR-4286 explained 5.5% (95% CI, 0.7%-17.0%) of the association of triglyceride with incident ACS. Conclusions Higher level of plasma miR-4286 was associated with an increased risk of ACS. The upregulated miR-4286 in plasma can be attributed to higher triglyceride level and may mediate the effect of triglyceride on incident ACS.
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http://dx.doi.org/10.1161/JAHA.120.018999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174203PMC
March 2021

Resilience patterns and transitions in the Be Resilient To Breast Cancer trial: an exploratory latent profile transition analysis.

Psychooncology 2021 Jun 10;30(6):901-909. Epub 2021 Mar 10.

South China University of Technology, Guangzhou, Guangdong Province, China.

Objective: Be Resilient to Breast Cancer (BRBC), a theoretically-derived, resilience-based, culturally-tailored, supportive-expressive group therapy (SEGT), has been developed to help promote patients' resilience in breast cancer. Data from patients receiving BRBC intervention was utilized to explore and define characteristics of resilience patterns and their transitions over time.

Methods: Resilience was used as a primary outcome and 391 patients completed Resilience Scale Specific to Cancer at enrollment (T0), 2 months (T1), 6 months(T2), and 12 months (T3) after intervention. latent profile transition analysis was performed to model the change in resilience and predict positive transitioning probabilities between resilience patterns (from one pattern to another pattern with a higher level) over time.

Results: One hundred and forty four resilience patterns were identified after BRBC intervention. 33.1%, 50.3%, and 40.5% of patients experienced positive resilience transitions from T0 to T1, T1 to T2, and T2 to T3, respectively. Patients with middle age, unmarried status, higher education level, and less advanced tumor stage were more likely to experience positive resilience transitions.

Conclusion: Different transitions of resilience patterns are observed after BRBC intervention. Age, marital status, education, and tumor stage may be four factors affecting the efficacy of SEGT intervention in breast cancer.
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http://dx.doi.org/10.1002/pon.5668DOI Listing
June 2021

Oxidation of the inert sp C-H bonds of tetrahydroisoquinolines through C-H activation relay (CHAR): construction of functionalized isoquinolin-1-ones.

Chem Commun (Camb) 2021 Apr 4;57(27):3347-3350. Epub 2021 Mar 4.

School of Chemistry & Chemical Engineering, Yangzhou University, Siwangting Road 180, Yangzhou, Jiangsu 225002, China.

A TBN/O-initiated oxidation of the relatively inert 3,4-C-H bonds of THIQs was accomplished, in which the existence of an α-phosphoric ester group is crucial to enable dioxygen trapping and intramolecular HAT (C-H activation relay, CHAR), realizing the synthesis of a series of isoquinolin-1-ones in high yields. The mechanistic study confirmed that the formation of the 3,4-double bond is mediated by the CHAR process. This work provides a new strategy to achieve remote C-H bond activation.
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http://dx.doi.org/10.1039/d1cc00550bDOI Listing
April 2021

Washed microbiota transplantation reduces proton pump inhibitor dependency in nonerosive reflux disease.

World J Gastroenterol 2021 Feb;27(6):513-522

Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangzhou 510030, Guangdong Province, China.

Background: The pathogenesis of gastroesophageal reflux disease (GERD) is closely associated with the intestinal bacteria composition and their metabolites.

Aim: To investigate whether washed microbiota transplantation (WMT) improves symptoms of nonerosive reflux disease (NERD) with proton pump inhibitor (PPI) dependency.

Methods: Patients with recurrent NERD and PPI dependency at the First Affiliated Hospital of Guangdong Pharmaceutical University from 2017 to 2018 were included and divided into a WMT or PPI group treated with PPI with/without WMT. The endpoint was NERD symptom frequency evaluated 1 mo after WMT using reflux disease questionnaire (RDQ) and GERD questionnaire (GERDQ) scores, remission time, PPI dose, and the examination of intestinal mucosal barrier function.

Results: In the WMT ( = 15) and PPI ( = 12) groups, the total remission rate at 1 mo after treatment was 93.3% 41.7%. Compared with the PPI group, the WMT group showed better results in GERDQ ( = 0.004) and RDQ ( = 0.003) and in remission months (8 2, = 0.002). The PPI dose was reduced to some extent for 80% of patients in the WMT group and 33.3% in the PPI group. In 24 patients, intestinal mucosal barrier function was examined before treatment, and changes in the degree of damage were observed in 13 of these patients after treatment. Only one of the 15 patients had minor side effects, including a mushy stool two or three times a day, which resolved on their own after 1 wk.

Conclusion: This study is the first to demonstrate that WMT may be safe and effective for relieving NERD symptoms and reducing PPI dependency and recurrence.
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http://dx.doi.org/10.3748/wjg.v27.i6.513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896436PMC
February 2021

Strong Triplet-Exciton-LO-Phonon Coupling in Two-Dimensional Layered Organic-Inorganic Hybrid Perovskite Single Crystal Microflakes.

J Phys Chem Lett 2021 Mar 24;12(8):2133-2141. Epub 2021 Feb 24.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Two-dimensional (2D) layered hybrid perovskites provide an ideal platform for studying the properties of excitons. Here, we report on a strong triplet-exciton and longitudinal-optical (LO) phonon coupling in 2D (CHCHCHNH, PEA)PbBr perovskites. The triplet excitons exhibit strong photoluminescence (PL) in thick perovskite microflakes, and the PL is not detectable for monolayer microflakes. The coupling strength of the triplet exciton-LO phonon is approximately two to three times greater than that of the singlet exciton-LO phonon with a LO phonon energy of about 21 meV. This difference might due to the different locations of singlet excitons located in the well and triplet excitons located in the barrier in the 2D layered perovskite. Revealing the strong coupling of triplet exciton-LO phonon provides a fundamental understanding of many-body interaction in hybrid perovskites, which is useful to develop and optimize the optoelectronic devices based on 2D perovskites in the future.
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http://dx.doi.org/10.1021/acs.jpclett.1c00342DOI Listing
March 2021

The peak levels of highly sensitive troponin I predicts in-hospital mortality in COVID-19 patients with cardiac injury: a retrospective study.

Eur Heart J Acute Cardiovasc Care 2021 Mar;10(1):6-15

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Ave, Wuhan, 430022 Hubei, China.

Aims: To investigate the association between levels of highly sensitive troponin I (hs-troponin I) and mortality in novel coronavirus disease 2019 (COVID-19) patients with cardiac injury.

Methods And Results: We retrospectively reviewed the medical records of all COVID-19 patients with increased levels of hs-troponin I from two hospitals in Wuhan, China. Demographic information, laboratory test results, cardiac ultrasonographic findings, and electrocardiograms were collected, and their predictive value on in-hospital mortality was explored using multivariable logistic regression. Of 1500 patients screened, 242 COVID-19 patients were enrolled in our study. Their median age was 68 years, and (48.8%) had underlying cardiovascular diseases. One hundred and seventy-six (72.7%) patients died during hospitalization. Multivariable logistic regression showed that C-reactive protein (>75.5 mg/L), D-dimer (>1.5 μg/mL), and acute respiratory distress syndrome were risk factors of mortality, and the peak hs-troponin I levels (>259.4 pg/mL) instead of the hs-troponin I levels at admission was predictor of death. The area under the receiver operating characteristic curve of the peak levels of hs-troponin I for predicting in-hospital mortality was 0.79 (95% confidence interval, 0.73-0.86; sensitivity, 0.80; specificity, 0.72; P < 0.0001).

Conclusion: Our results demonstrated that the risk of in-hospital death among COVID-19 patients with cardiac injury can be predicted by the peak levels of hs-troponin I during hospitalization and was significantly associated with oxygen supply-demand mismatch, inflammation, and coagulation.
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http://dx.doi.org/10.1093/ehjacc/zuaa019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665398PMC
March 2021

The lysosomotrope GPN mobilises Ca from acidic organelles.

J Cell Sci 2021 Mar 11;134(6). Epub 2021 Mar 11.

Department of Cell and Developmental Biology, UCL, London WC1E 6BT, UK

Lysosomes are acidic Ca stores often mobilised in conjunction with endoplasmic reticulum (ER) Ca stores. Glycyl-L-phenylalanine 2-naphthylamide (GPN) is a widely used lysosomotropic agent that evokes cytosolic Ca signals in many cells. However, whether these signals are the result of a primary action on lysosomes is unclear in light of recent evidence showing that GPN mediates direct ER Ca release through changes in cytosolic pH. Here, we show that GPN evoked rapid increases in cytosolic pH but slower Ca signals. NHCl evoked comparable changes in pH but failed to affect Ca The V-type ATPase inhibitor, bafilomycin A1, increased lysosomal pH over a period of hours. Acute treatment modestly affected lysosomal pH and potentiated Ca signals evoked by GPN. In contrast, chronic treatment led to more profound changes in luminal pH and selectively inhibited GPN action. GPN blocked Ca responses evoked by the novel nicotinic acid adenine dinucleotide phosphate-like agonist, TPC2-A1-N. Therefore, GPN-evoked Ca signals were better correlated with associated pH changes in the lysosome compared to the cytosol, and were coupled to lysosomal Ca release. We conclude that Ca signals evoked by GPN most likely derive from acidic organelles.
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http://dx.doi.org/10.1242/jcs.256578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972315PMC
March 2021

MicroRNA expression profiles analysis of apheresis platelets treated with vitamin B and ultraviolet-B during storage.

Transfus Apher Sci 2021 Feb 8:103079. Epub 2021 Feb 8.

Institute of Hematology, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China. Electronic address:

Whether platelet (PLT) microRNA (miRNA) profiles are affected by pathogen reduction technology (PRT) using vitamin B and ultraviolet-B (VB-PRT) remains unclear. Samples from VB-PRT-treated (experimental group, E_) and untreated (control group, C_) apheresis PLTs were taken on days 1, 3 and 5 of storage, designated as E_1, E_3, E_5, C_1, C_3 and C_5, respectively. The miRNA expression profiles were assessed by DNA Nano Ball (DNB) sequencing technology, and verified by quantitive real-time fluorescence quantitative PCR (qRT-PCR). Compared with the expression profiles of PLT miRNAs, 3895 miRNAs were identified in the E_ groups while 4106 were in the C_ groups. There were 487 significant differentially expressed miRNAs in E_1 vs C_1 group, including 220 upregulated and 287 downregulated, such as miR-146a-5p and let-7b-5p. There were 908 significant differentially expressed miRNAs in E_3 vs C_3 group, including 297 upregulated and 611 downregulated, such as miR-142-5p and miR-7-5p. There were 229 significant differentially expressed miRNAs in E_5 vs C_5 group, including 80 upregulated and 149 downregulated, such as miR-3529-3p and miR-451a. These differentially expressed miRNAs had been suggested to have functional roles in energy homeostasis, cell communication, proliferation, migration and apoptosis. GO analysis showed a significant enrichmen in relevant biological process categories as receptor activity, signal transduction, cell transport, motility and chemotaxis. The significantly enriched KEGG pathway of predicted target genes was Glycosaminoglycan biosynthesis in E_ vs C_ groups. These new observation could provide insights on the understanding of change of miRNA profiles of PLT treated with VB-PRT.
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http://dx.doi.org/10.1016/j.transci.2021.103079DOI Listing
February 2021