Publications by authors named "Yuan Yang"

1,632 Publications

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Diastereoselective Synthesis of Bicyclo[3.3.0]octenones by Copper-Catalyzed Transannular Ring-Closing Reaction.

Org Lett 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

A novel and efficient copper-catalyzed transannular ring-closing reaction of eight-membered rings has been developed that provides a straightforward way to synthesize bicyclo[3.3.0]octane derivatives in good yields. Mechanistic studies revealed that the reaction pathway might involve chlorination followed by the Kornblum reaction. Readily accessible starting materials and good functional group tolerance make this procedure attractive.
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http://dx.doi.org/10.1021/acs.orglett.2c02163DOI Listing
August 2022

Prognosis of systemic inflammation at an early stage of cirrhosis using the monocyte-to-lymphocyte ratio during malnutrition risk screening: a prospective cohort study.

Postgrad Med 2022 Aug 5. Epub 2022 Aug 5.

Department of Infectious Diseases and Hepatopathy, The First Affiliated Hospital of Xi'an Jiaotong University, China.

Objectives: To determine whether the monocyte-to-lymphocyte ratio (MLR), as a systemic inflammation index, predicts malnutrition risk during the early stages of cirrhosis.

Methods: We conducted a single-center prospective cohort study, enrolling patients from June 2016 to September 2020. The patients underwent malnutrition risk assessments upon admission. The patients were classified into five clinical stages according to portal hypertension. The malnutrition risk was scored using the Royal Free Hospital-Nutritional Prioritizing Tool (RFH-NPT) and validated by the Nutritional Risk Screening 2002 (NRS-2002) or Liver Disease Undernutrition Screening Tool (LDUST). Routine clinical laboratory measurements were performed to calculate the MLR, Child-Turcotte-Pugh (CTP) class, and model for end-stage liver disease (MELD) score. The patients were followed up for 2 years.

Results: Among the 154 patients with cirrhosis, 60 had compensated cirrhosis and 94 had decompensated cirrhosis. The optimal cutoff value of the MLR, >0.4, was effective in predicting malnutrition related to death or liver transplantation. Those with a high malnutrition risk defined by the NRS-2002 or RFH-NPT had a higher MLR than those with a low malnutrition risk. For patients with class A CTP cirrhosis or a MELD score of <10, an MLR cutoff of <0.4 significantly distinguished more patients with a low malnutrition risk than those with a high malnutrition risk. Both the RFH-NPT score and MLR increased significantly across the decompensated cirrhosis substages. Interestingly, the MLR exhibited a positive correlation with the RFH-NPT score until varices appeared, but the correlation was the highest at the substage of a history of variceal bleeding (r = 0.714, P = 0.009). Multivariable analysis demonstrated that an MLR of >0.4 was an independent factor for malnutrition risk by screening with the RFH-NPT, and this was confirmed using the LDUST and NRS-2002.

Conclusion: Immune-related inflammatory dysfunction predicts malnutrition risk during the early stages of cirrhosis.
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http://dx.doi.org/10.1080/00325481.2022.2110600DOI Listing
August 2022

Direct Observation of Li-Ion Transport Heterogeneity Induced by Nanoscale Phase Separation in Li-rich Cathodes of Solid-State Batteries.

Angew Chem Int Ed Engl 2022 Aug 5. Epub 2022 Aug 5.

Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences, Department of Energy Science and Energy Technology, Songling Road, 189, 266101, Qingdao City, CHINA.

Li-rich layered oxide (LLO) cathode materials with high specific capacities could significantly enhance the energy density of all-solid-state lithium batteries (ASSLBs). However, the specific practical capacities of LLO materials in ASSLBs are extremely low due to poor initial activation. Here, scanning transmission electron microscopy with in-situ differential phase contrast imaging was first used to study the initial activation mechanism of Li1.2Ni0.13Co0.13Mn0.54O2. Li-ion transport heterogeneity was observed in LLO grains and across the LLO/Li6PS5Cl interface, due to the coexistence of the nanoscale Li2MnO3 and LiNi1/3Co1/3Mn1/3O2 phases. Consequently, the severely constrained activation of Li2MnO3 during the first charging could be attributed to a nanoscale phase separation in LLO, hindering Li-ion transport through its particles, and causing high impedance in the Li2MnO3 domain/Li6PS5Cl interface. This study could facilitate interface design of high-performance LLO-based ASSLBs.
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http://dx.doi.org/10.1002/anie.202209626DOI Listing
August 2022

Perfluorononanoate and perfluorobutane sulfonate induce cardiotoxic effects in zebrafish.

Environ Toxicol Chem 2022 Jul 28. Epub 2022 Jul 28.

Clinical Research Center, Wuhan Children's Hospital (Wuhan Medical and Health Center for Women and Children), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430016, Hubei, People's Republic of China.

Globally, per- and polyfluoroalkyl substances (PFASs) are common artificial ingredients in industrial and consumer products. Recently, they have been shown to be an emerging human health risk. Perfluorononanoic acid (PFNA)/perfluorononanoate and perfluorobutane sulfonic acid (PFBS)/perfluorobutane sulfonate cause reproductive toxicity and hepatotoxicity, disrupt thyroid functions, and damage embryonic development in zebrafish. However, cardiotoxic effects of PFNA and PFBS have not been fully established. We found that PFNA and PFBS exposures repress hatchability while increasing malformation and mortality in zebrafish embryos. Hematoxylin and eosin staining as well as assessment of the transgenic zebrafish line Tg(myl7:nDsRed) revealed that exposures of embryos to PFNA increases the occurrence of severe cardiac malformations, relative to exposure to PFBS. Moreover, we evaluated the differential expressions of cardiac development-associated genes in response to PFNA and PFBS, which validated the potential cardiotoxic effects, consistent with cardiac dysfunctions. Overall, our findings reveal novel cardiotoxic effects of PFNA and PFBS in zebrafish, implying that they may exert some cardiotoxic effect in humans. To the best of our knowledge, this is the first study to show that PFNA exerts more severe cardiotoxic effects in zebrafish, when compared to PFBS. Based on these findings, studies should evaluate the mechanisms of their cardiotoxic effects. This article is protected by copyright. All rights reserved. © 2022 SETAC.
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http://dx.doi.org/10.1002/etc.5447DOI Listing
July 2022

Immunoprotective Effects of Two Histone H2A Variants in the Grass Carp Against Infection.

Front Immunol 2022 11;13:939464. Epub 2022 Jul 11.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

In teleost fish, the nucleotide polymorphisms of histone H2A significantly affect the resistance or susceptibility of zebrafish to infection. Whether histone H2A variants can enhance the resistance of grass carp to infection remains unclear. Here, the effects of 7 previously obtained variants (gcH2A-1~gcH2A-7) and 5 novel histone H2A variants (gcH2A-11, gcH2A-13~gcH2A-16) in response to infection were investigated. It was found that these histone H2A variants could be divided into type I and II. Among them, 5 histone H2A variants had no any effects on the infection, however 7 histone H2A variants had antibacterial activity against infection. The gcH2A-4 and gcH2A-11, whose antibacterial activity was the strongest in type I and II histone H2A variants respectively, were picked out for yeast expression. Transcriptome data for the samples from the intestines of grass carp immunized with the engineered expressing PYD1, gcH2A-4 or gcH2A-11 revealed that 5 and 12 immune-related signaling pathways were significantly enriched by gcH2A-4 or gcH2A-11, respectively. For the engineered expressing gcH2A-4, NOD-like receptor and Toll-like receptor signaling pathways were enriched for up-regulated DEGs. Besides NOD-like receptor and Toll-like receptor signaling pathways, the engineered expressing gcH2A-11 also activated Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway and C-type lectin receptor signaling pathway. Furthermore, grass carp were immunized with the engineered expressing PYD1, gcH2A-4 or gcH2A-11 for 1 month and challenged with These grass carp immunized with gcH2A-4 or gcH2A-11 showed lower mortality and fewer numbers of than did the control group. All these results suggest that gcH2A-4 and gcH2A-11 play important roles in evoking the innate immune responses and enhancing disease resistance of grass carp against infection.
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http://dx.doi.org/10.3389/fimmu.2022.939464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310644PMC
July 2022

Role of Pyroptosis in Gynecological Oncology and Its Therapeutic Regulation.

Biomolecules 2022 07 1;12(7). Epub 2022 Jul 1.

The Reproductive Medicine Center, The 1st Hospital of Lanzhou University, Lanzhou 730000, China.

With the continuous advances in molecular biotechnology, many new cell death methods have been discovered. Pyroptosis is a programmed cell death process that differs from apoptosis and autophagy in cell morphology and function. Compared with apoptosis and autophagy, pyroptosis is primarily mediated by intracellular inflammasome and gasdermin D of the gasdermin protein family and involves the release of numerous inflammatory factors. Pyroptosis has been found to be involved in the occurrence and development of infectious diseases and other diseases involving the nervous system and the cardiovascular system. Recent studies have also reported the occurrence of pyroptosis in tumor cells. Accordingly, exploring its effect on tumors has become one of the research hotspots. Herein, recent research progress on pyroptosis is reviewed, especially its role in the development of gynecological tumors. As the pathogenesis of gynecological tumor is better understood, new targets have been introduced for the prevention and clinical treatment of gynecological tumors.
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http://dx.doi.org/10.3390/biom12070924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313147PMC
July 2022

Generating Synthesized Ultrasound Biomicroscopy Images from Anterior Segment Optical Coherent Tomography Images by Generative Adversarial Networks for Iridociliary Assessment.

Ophthalmol Ther 2022 Jul 26. Epub 2022 Jul 26.

Department of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 1665, Kongjiang Road, Shanghai, 200092, China.

Introduction: The aim of this study was to investigate the feasibility of generating synthesized ultrasound biomicroscopy (UBM) images from swept-source anterior segment optical coherent tomography (SS-ASOCT) images using a cycle-consistent generative adversarial network framework (CycleGAN) for iridociliary assessment on a cohort presenting for primary angle-closure screening.

Methods: The CycleGAN architecture was adopted to synthesize high-resolution UBM images trained on the SS-ASOCT dataset from the department of ophthalmology, Xinhua Hospital. The performance of the CycleGAN model was further tested in two separate datasets using synthetic UBM images from two different ASOCT modalities (in-distribution and out-of-distribution). We compared the ability of glaucoma specialists to assess the image quality of real and synthetic images. UBM measurements, including anterior chamber, iridociliary parameters, were compared between real and synthetic UBM images. Intra-class correlation coefficients, coefficients of variation, and Bland-Altman plots were used to assess the level of agreement. The Fréchet Inception Distance (FID) was measured to evaluate the quality of the synthetic images.

Results: The whole trained dataset included anterior chamber angle images, of which 4037 were obtained by SS-ASOCT and 2206 were obtained by UBM. The image quality of real versus synthetic SS-ASOCT images was similar as assessed by two glaucoma specialists. The Bland-Altman analysis also suggested high consistency between measurements of real and synthetic UBM images. In addition, there was fair to excellent agreement between real and synthetic UBM measurements for the in-distribution dataset (ICC range 0.48-0.97) and the out-of-distribution dataset (ICC range 0.52-0.86). The FID was 21.3 and 24.1 for the synthetic UBM images from the in-distribution and out-of-distribution datasets, respectively.

Conclusion: We developed a CycleGAN model to translate UBM images from non-contact SS-ASOCT images. The CycleGAN synthetic UBM images showed fair to excellent reproducibility when compared with real UBM images. Our results suggest that the CycleGAN technique is a promising tool to evaluate the iridociliary and anterior chamber in an alternative non-contact method.
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http://dx.doi.org/10.1007/s40123-022-00548-1DOI Listing
July 2022

Iterative Synthesis of Contorted Macromolecular Ladders for Fast-Charging and Long-Life Lithium Batteries.

J Am Chem Soc 2022 Aug 25;144(30):13973-13980. Epub 2022 Jul 25.

Department of Chemistry, Columbia University, New York, New York 10027, United States.

We report here an iterative synthesis of long helical perylene diimide () nanoribbons with a length up to 16 fused benzene rings. These contorted, ladder-type conjugated, and atomically precise nanoribbons show great potential as organic fast-charging and long-lifetime battery cathodes. By tuning the length of the oligomers, we can simultaneously modulate the electrical conductivity and ionic diffusivity of the material. The length of the ladders adjusts both the conjugation for electron transport and the contortion for lithium-ion transport. The longest oligomer, , when fabricated as the cathode in lithium batteries, features both high electrical conductivity and high ionic diffusivity. This electrode material exhibits a high power density and can be charged in less than 1 min to 66% of its maximum capacity. Remarkably, this material also has exceptional cycling stability and can operate for up to 10,000 charging-discharging cycles without any appreciable capacity decay. The design principles described here chart a clear path for organic battery electrodes that are sustainable, fast-charging, and long lasting.
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http://dx.doi.org/10.1021/jacs.2c06527DOI Listing
August 2022

Identification of sugar transporter (SWEET) genes involved in pomegranate seed coat sugar accumulation.

3 Biotech 2022 Sep 19;12(9):181. Epub 2022 Jul 19.

Key Laboratory of Horticultural Crop Genetic Improvement and Eco-Physiology of Anhui Province, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei, 230031 China.

Sugar content of the outer seed coat and hardness of the inner seed coat are important traits of the pomegranate fruit. The translocation of sugars across biological membranes, mediated by SWEET transporters, is critical to seed development. In this study, we identified 16 PgrSWEET genes distributed on six chromosomes in the pomegranate genome. According to the phylogenetic analysis, PgrSWEET proteins were divided into four groups. Tandem and segmental duplications contributed to the expansion of the PgrSWEET family, while functional redundancy and diversification may have occurred among SWEET members according to analyses of evolution and gene expression. RNA-seq and qRT-PCR analyses revealed that PgrSWEET1a and PgrSWEET9 were highly expressed in the inner seed coat, and the expression levels gradually increased during seed development. Moreover, the relative expression levels of PgrSWEET1a and PgrSWEET9 in a hard-seeded cultivar were higher than those in a soft-seeded cultivar, indicating that PgrSWEET1a and PgrSWEET9 might function in the inner seed coat development by accumulating sugar metabolites. We also found that PgrSWEET2 was highly expressed in the outer seed coat during seed development, and the protein was localized to the tonoplast, indicating that PgrSWEET2 is likely a candidate regulating sugar accumulation or reutilization in the vacuoles of the outer seed coat. Genes encoding transcription factors probably regulating the candidate PgrSWEET genes were chosen by co-expression analysis. These results not only helped to characterize PgrSWEET genes but also provided an insight into their functions in relation to seed coat development.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03248-6.
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http://dx.doi.org/10.1007/s13205-022-03248-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296756PMC
September 2022

Cyclophosphamide for Treatment of Refractory Chronic Inflammatory Demyelinating Polyradiculoneuropathy: A Systematic Review and Meta-analysis.

Clin Ther 2022 Jul 21. Epub 2022 Jul 21.

Department of Rheumatology and Immunology, Nanchong Central Hospital, The Affiliated Nanchong Central Hospital of North Sichuan Medical College, Nanchong, China; Inflammation and Immunology Key Laboratory of Nanchong City, Sichuan, China. Electronic address:

Purpose: This study evaluates the tolerability and efficacy of cyclophosphamide (CYC) for the treatment of refractory chronic inflammatory demyelinating polyradiculoneuropathy (CIDP).

Methods: We searched PubMed, Embase, Cochrane Library, and 2 Chinese databases (Chinese National Knowledge Infrastructure and SinoMed) for studies published between database inception and September 30, 2021. Articles obtained using the appropriate keywords were independently selected by 2 reviewers on the basis of the established inclusion and exclusion criteria.

Findings: In total, 240 records that were eventually curtailed to 13 studies with 83 patients were retrieved and subsequently included in this evaluation. All 13 studies were included in the systematic review, 7 of which were included in the meta-analysis. The pooled estimate of the response rate was 68% (95% CI, 45%-90%). The pooled estimate of the adverse reaction rate was 8% (95% CI, 0%-15%). The disease duration before the first CYC treatment was negatively correlated with the reduction in the modified Rankin Scale score (r = -0.76, P < 0.001). However, the response rates did not differ significantly between patients of different sexes (P = 0.716) or between patients who received and those who did not receive concurrent glucocorticoids (P = 0.617).

Implications: CYC might be a recommended therapeutic option for patients with refractory CIDP, especially those who are unable to accept rituximab treatment. Earlier CYC treatment yields better therapeutic outcomes in patients with refractory CIDP without CYC-related contraindications.
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http://dx.doi.org/10.1016/j.clinthera.2022.06.008DOI Listing
July 2022

[Development and Evaluation of Prognostic Nomogram Model for Adult Ventricle Glioma Patients].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 Jul;53(4):588-596

Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To explore the prognostic factors of adult ventricle glioma (AVG) and to construct and evaluate a survival-related prognostic nomogram model, which could provide further reference for the clinical management of AVG patients.

Methods: The patients covered in the study were selected from the Surveillance Epidemiology and End Results (SEER) database (1973-2016). They all had definite histological diagnosis of AVG. They were assigned randomly to the training cohort and the validation cohort by random number table at a 2/1 ratio. Survival analysis was performed by Kaplan-Meier analysis. Cox regression analysis was employed to determine the independent prognostic factors for overall survival (OS) and cancer-specific survival (CSS). Then, integrating the basic characteristics of patients, the survival-related nomogram predictive model for OS and CSS in the training cohort was constructed, respectively. After that, internal cross validation and external validation of the model were carried out with the training cohort and the validation cohort in succession. The authenticity and reliability of the nomogram model were evaluated by calculating the concordance index (C-index). Calibration plots were constructed to assess the agreement between the predicted values and the observed values in the training cohort and the validation cohort.

Results: A total of 369 AVG patients, including 218 males and 151 females, were included. The median age of the patients was 53. According to the WHO classification of gliomas, 66 (17.9%) patients had grade Ⅱ gliomas, 73 (19.8%) had grade Ⅲ gliomas, and 230 (62.3%) had grade Ⅳ gliomas. Regarding the extent of resection (EOR), 59 (16.0%) had gross total resection (GTR) and 145 (39.3%) had subtotal resection (STR) or partial resection (PR). Of all the patients, 167 (45.3%) received postoperative radiotherapy and 143 (38.8%) received postoperative chemotherapy. Patients were randomized into the training cohort ( =246) and the validation cohort ( =123), and there was no significant difference ( >0.05) in the basic clinical characteristics between the training cohort and the validation cohort. In the training cohort, Cox regression analysis revealed that the independent prognostic factors for OS and CSS included age≥65, grades Ⅲ and Ⅳ according to the WHO classification of gliomas, and not receiving radiotherapy. Furthermore, 5 variables, including age, gender, WHO grades, surgery, and radiotherapy, were used to construct the nomogram model for predicting 6-month, 1-year, and 2-year OS and CSS. The results of internal cross validation in the training cohort showed that the C-indexes of OS and CSS were 0.758 and 0.765, respectively. The external validation results of the validation cohort showed that the C-indexes of OS and CSS were 0.733 and 0.719, respectively. Calibration plots for 6-month, 1-year, and 2-year OS in the training cohort showed relatively good agreement, while in the validation cohort the agreement was relatively low. The 6-month, 1-year, and 2-year CSS calibration plots had results similar to the calibration plots of OS.

Conclusion: This nomogram predictive model of OS and CSS showed moderately reliable predictive performance, providing helpful reference information for clinicians to make quick and simple assessment of the survival probability of AVG patients.
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http://dx.doi.org/10.12182/20220760203DOI Listing
July 2022

Research advances in palladium-catalysed intermolecular C-H annulation of aryl halides with various aromatic ring precursors.

Org Biomol Chem 2022 Jul 21. Epub 2022 Jul 21.

National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), and Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, Hunan Normal University, Changsha 410081, China.

Transition-metal-catalysed C-H functionalization has emerged as a powerful approach for the transformation of organic molecules due to its high atom and step economy. Palladium-catalysed intermolecular C-H annulation of aryl halides, especially those involving annulation of a five-membered ,-palladacycle with coupling reagents, have attracted considerable attention in the past decades. This review summarizes the progress on palladium-catalysed intermolecular C-H annulation of aryl halides with various aromatic ring precursors. Mechanistically, five-membered ,-palladacycles as intermediates are involved in the majority of reactions.
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http://dx.doi.org/10.1039/d2ob01129hDOI Listing
July 2022

A Novel MG 2D Animation Design Method under the Perspective of Convergence Media Using Intelligent Design Technology.

Comput Intell Neurosci 2022 9;2022:2568690. Epub 2022 Jul 9.

Institute of Art and Design, Nanjing Institute of Technology, Nanjing, Jiangsu 211167, China.

The arrival of the 5G era, along with the gradual acceleration of information technology development, makes all kinds of new media gradually integrated into all corners of people's life, work, and study. Communication systems are breaking the original boundaries and moving toward communion. "Convergence Media" is a general term of new media which is built on modern network technology and integrates various media forms. In this context, animation design not only is limited to two-dimensional or three-dimensional creation, but also formed a new form of animation expression, namely, MG animation. For MG animation, with its simple artistic modeling elements and flexible rhythm, people have gained new cognition. When the Internet information is transmitted, MG animation can output a large number of dynamic images and text to people in a very short time, which is similar to the current mode of new media communication. It can make information more attractive and appealing with the visual performance of MG animation. In order to improve the quality and efficiency of animation, convergence media technology is applied to the drawing and production of animation. The emergence and continuous improvement of software such as AE, PR, and PS and other software have a strong compatibility and can fully meet our various requirements for MG animation production, thus bringing the audience a different kind of animation experience. AE, a common software for video production, can create a variety of striking visual effects with high efficiency and precision, with powerful effects control and millions of plug-ins to achieve a wide range of animation effects. High-quality video rendering supports resolutions from 4 × 4 to 30,000 × 30,000 pixels including high-definition television (HDTV). Therefore, in terms of animation production, we can choose to mainly use AE for production and rendering. In terms of drawing tools, PS is the software of choice, with no delay in drawing, dithering correction, selection, and other features which are quite outstanding, and has a great advantage in drawing lines and coloring. Based on this, this paper mainly explores the design and production cases of MG 2D animation from the new media perspective using the intelligent design technology. The animation designed by the method in this paper can also be used for medical treatment management or lesion display in the medical field.
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http://dx.doi.org/10.1155/2022/2568690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288329PMC
July 2022

Related factors to the coping style of patients with sudden sensorineural hearing loss.

Int Health 2022 Jul 18. Epub 2022 Jul 18.

First Affiliated Hospital of Soochow University, Suzhou 215006, China.

Background: Coping style can affect the patient's physical and mental health management. Therefore this study aimed to identify factors related to the coping style of young and middle-aged sudden sensorineural hearing loss (SSNHL) patients to provide reference for clinical nursing practice.

Methods: A cross-sectional study was conducted on young and middle-aged SSNHL patients hospitalized in the otolaryngology departments of four hospitals in Suzhou City, China. A paper-based self-administered questionnaire investigated the patient's coping style and related factors. Multiple linear stepwise regression analysed the effective related factors in patients' coping styles.

Results: Among 872 patients, 866 completed the survey, with an average age of 37.27 y. Factors related to the coping style of these patients included gender, chronic diseases, history of trauma, social support and type D personality (p<0.05). Female patients adopt more negative coping styles than male patients. Patients with chronic diseases or a history of trauma had more positive coping styles. Higher social support scores were related to improvements in coping style. Patients with type D personality were more likely to adopt negative coping styles.

Conclusions: This study suggests that psychological assessment of patients, chronic diseases, history of trauma, social support and type D personality may benefit the understanding of these patients' coping styles and, as a consequence, may improve their stress management.
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http://dx.doi.org/10.1093/inthealth/ihac046DOI Listing
July 2022

Seroprevalence and dynamics of anti-SARS-CoV-2 antibodies: a longitudinal study based on patients with underlying diseases in Wuhan.

Respir Res 2022 Jul 15;23(1):188. Epub 2022 Jul 15.

School of Population Medicine and Public Health, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Assessing the humoral immunity of patients with underlying diseases after being infected with SARS-CoV-2 is essential for adopting effective prevention and control strategies. The purpose of this study is to analyze the seroprevalence of people with underlying diseases and the dynamic change features of anti-SARS-CoV-2 antibodies.

Methods: We selected 100 communities in Wuhan using the probability-proportional-to-size sampling method. From these 100 communities, we randomly selected households according to a list provided by the local government. Individuals who have lived in Wuhan for at least 14 days since December 2019 and were ≥ 40 years old were included. From April 9-13, 2020, community staff invited all selected individuals to the community healthcare center in batches by going door-to-door or telephone. All participants completed a standardized electronic questionnaire simultaneously. Finally, 5 ml of venous blood was collected from all participants. Blood samples were tested for the presence of pan-immunoglobulins, IgM, IgA, and IgG antibodies against SARS-CoV-2 nucleocapsid protein and neutralising antibodies were assessed. During the period June 11-13, 2020 and October 9-December 5, 2020, all family members of a positive family and matched negative families were followed up twice.

Results: The seroprevalence of anti-SARS-CoV-2 antibodies in people with underlying diseases was 6.30% (95% CI [5.09-7.52]), and that of people without underlying diseases was 6.12% (95% CI [5.33-6.91]). A total of 313 people were positive for total antibodies at baseline, of which 97 had underlying disease. At the first follow-up, a total of 212 people were positive for total antibodies, of which 66 had underlying disease. At the second follow-up, a total of 238 people were positive for total antibodies, of which 68 had underlying disease. A total of 219 participants had three consecutive serum samples with positive total antibodies at baseline. The IgG titers decreased significantly with or without underlying diseases (P < 0.05) within the 9 months at least, while the neutralizing antibody titer remained stable. The titer of asymptomatic patients was lower than that of symptomatic patients (baseline, P = 0.032, second follow-up, P = 0.018) in the underlying diseases group.

Conclusion: Our research focused on the serological changes of people with and without underlying diseases in a state of single natural infection. Regardless of the underlying diseases, the IgG titer decreased significantly over time, while there was no significant difference in the decline rate of IgG between with and without underlying diseases. Moreover, the neutralizing antibody titer remained relatively stable within the 9 months at least.
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http://dx.doi.org/10.1186/s12931-022-02096-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284953PMC
July 2022

A novel missense variant c.71G > T (p.Gly24Val) of the CRYBA4 gene contributes to autosomal-dominant congenital cataract in a Chinese family.

Int Ophthalmol 2022 Jul 16. Epub 2022 Jul 16.

Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.

Purpose: To investigate the potential genetic defects in a five-generation Chinese family with autosomal dominant congenital cataract (ADCC).

Methods: Whole exome sequencing was performed to search the variants in the candidate genes associated with congenital cataract. Sanger sequencing was used to validate the variants and examine their co-segregation in the patients and their relatives. The potential effect of the variants was analyzed using several bioinformatic methods and further examined through Western blotting and co-immunoprecipitation.

Results: A missense variant c. 71 G > T (p. Gly24Val) in the CRYBA4 gene, a known ADCC candidate gene, was identified to be heterozygously present in the patients and co-segregate with cataract in the family. The mutation was absent in all of the searched databases, including our in-house exome sequences of 10,000 Chinese. The alignments of the amino acid sequences of CRYBA4 in a variety of species revealed that the amino acid residue Gly24 was evolutionarily highly conserved, and the in silico analysis predicted that the missense mutation of Gly24Val was damaging for the protein structure and function of CRYBA4. Then, the in vitro expression analysis further revealed that the Gly24Val mutation in CRYBA4 inhibited its binding with CRYBB1. The impaired interaction of β-crystallin proteins may affect their water-solubility and contribute to the formation of precipitates in lens fiber cells.

Conclusion: We identified a novel missense variant in the CRYBA4 gene as a pathogenic mutation of ADCC in a Chinese family. Our finding expanded the CRYBA4 variation spectrum associated with congenital cataracts.
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http://dx.doi.org/10.1007/s10792-022-02386-3DOI Listing
July 2022

Clinical Characteristics of Anti--Methyl-d-Aspartate Receptor Encephalitis Overlapping with Demyelinating Diseases: A Review.

Front Immunol 2022 28;13:857443. Epub 2022 Jun 28.

Department of Neurology, West China Hospital of Sichuan University, Chengdu, China.

Anti--methyl-d-aspartate receptor encephalitis (NMDARe), a common autoimmune encephalitis, can be accompanied by demyelinating disorders, including multiple sclerosis (MS), neuromyelitis optica spectrum disorder (NMOSD), and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). To compare the clinical characteristics of patients with different overlapping syndromes, we searched the PubMed database and performed a systematic review. Of the 79 patients with overlapping syndromes, 15 had MS, 18 had aquaporin-4-antibody-positive NMOSD (AQP4-Ab-positive NMOSD), and 46 had MOGAD. Compared with classical NMDARe, overlapping syndromes showed atypical symptoms, such as limb weakness, sensory disturbance, and visual impairments in addition to the main symptoms of NMDARe and a lower ratio of ovarian teratoma. Patients with MOGAD overlap were the youngest, while patients with MS and AQP4-Ab-positive NMOSD overlap tended to be older than patients with classical NMDARe. A majority of patients with NMDARe who overlapped with MS or AQP4-Ab-positive NMOSD were female, but this was not the case for patients overlapped with MOGAD. When NMDARe and demyelinating diseases occurred sequentially, the interval was the longest in patients with NMDARe overlapped with MS. A favorable outcome was observed in patients overlapping with MOGAD, but no robust comparison can be drawn with the patients overlapping with AQP4-Ab-positive NMOSD and MS regarding the small number of available data. The long-term prognosis of overlapping syndromes needs further investigation.
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http://dx.doi.org/10.3389/fimmu.2022.857443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273846PMC
July 2022

Antidiabetic treatment improves prognosis after radical resection in hepatocellular carcinoma patients with diabetes mellitus: a retrospective cohort study from 2000 to 2013.

J Gastrointest Oncol 2022 Jun;13(3):1330-1339

Department of Endocrinology, Changhai Hospital, Naval Medical University, Shanghai, China.

Background: It remains unclear whether diabetic medications, such as metformin and insulin, affect the post-liver resection prognosis of hepatocellular carcinoma (HCC) patients complicated with diabetes mellitus (DM). This study try to find out the prognostic factors in HCC patients with DM and provide a better antidiabetic therapy after liver resection.

Methods: Patients presenting with HCC complicated with DM undergoing liver resection were enrolled in this study. They were examined and followed up every 3-6 months after surgery. Patients were divided into the antidiabetic treatment group and no antidiabetic treatment group according to whether they received medications for diabetes or not. Then patients in the antidiabetic treatment group were further divided into insulin group, metformin group, insulin plus metformin group and others group, according to the medications they received. Overall survival (OS) and recurrence-free survival (RFS) were compared among two groups and four subgoups. Comparative and multivariate analyses were performed to investigate the effects of DM medication on the prognosis of these HCC patients, using Cox proportional hazards model.

Results: The 1-, 3-, 5-, and 7-year OS rates for the antidiabetic treatment group were 87.5%, 75.5%, 48.7%, and 29.1%, respectively, and for the no antidiabetic treatment group, the OS rates were 85.4%, 57.7%, 33.6%, and 19.1%, respectively (P0.007). The 1-, 3-, 5-, 7-year RFS rates for the antidiabetic treatment group were 76.4%, 53.5%, 28.5%, and 17.5%, respectively, and for the no antidiabetic treatment group, the RFS rates were 69.5%, 32.5%, 16.5%, and 10.7%, respectively (P0.001). In subgroup analysis, There was no significant difference in either RFS (P0.934) nor OS (P0.412) among the different types of antidiabetic treatment regimens. Cox proportional hazard regression analysis revealed that tumor size (HR: 1.048), tumor number (HR: 1.626), vascular invasion (HR: 2.074, P=0.003), satellite tumor (HR: 1.592), Edmondson classification (HR: 1.468) and antidiabetic treatment (HR: 0.722) were independent prognostic factors of DFS, while tumor size (HR: 1.048), tumor number (HR: 1.779), vascular invasion (HR: 2.545), Edmondson classification (HR: 1.596) and antidiabetic treatment (HR: 0.713) were independent prognostic factors of OS.

Conclusions: For HCC patients with DM, antidiabetic treatment should be recommended aggressively in order to improve the surgical outcome, regardless of which antidiabetic drugs are used.
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http://dx.doi.org/10.21037/jgo-22-478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274028PMC
June 2022

Aromatherapy with single essential oils can significantly improve the sleep quality of cancer patients: a meta-analysis.

BMC Complement Med Ther 2022 Jul 14;22(1):187. Epub 2022 Jul 14.

The First Affiliated Hospital of Soochow University/School of Nursing, Soochow University, No. 188 Shizi Street, Suzhou, 215006, China.

Objective: To investigate the effect of aromatherapy on sleep quality in cancer patients.

Methods: Published literature on the effect of aromatherapy in cancer patients with sleep disorders in the form of randomized controlled trials (RCTs) were systematically retrieved and screened from PubMed, Cochrane Library, Embase, CBM, CNKI, VIP, and Wanfang databases from inception to November 2021. The methodological quality of the included studies was critically and independently evaluated by two reviewers using the Cochrane Risk of Bias Assessment Tool for RCTs. The correlated data were extracted using the pre-designed form, and all analyses were performed using Reviewer Manager version 5.4. Due to the difference in sleep quality instruments, the data extracted in this study were in the form of standard mean difference (SMD).

Results: Ten RCTs included 933 patients (experimental group: 474, control group: 459), and the risk of bias in the included studies was moderate. Aromatherapy could significantly improve the sleep quality of cancer patients [SMD = - 0.79, 95% CI (- 0.93, - 0.66), p < 0.01], especially those with breast cancer [SMD = - 0.98, 95% CI (- 1.57, - 0.40), p < 0.01]. Aromatherapy with single essential oil had a better effect on sleep quality [SMD = -0.94, 95%CI (- 1.25, - 0.62), p < 0.01], of which lavender essential oil had the best effect [SMD = -1.06,95%CI (- 1.49, - 0.63), p < 0.01] while compound essential oils had no effect on sleep quality improvement in cancer patients [SMD = -0.21, 95%CI (- 0.57, 0.14), p = 0.23]. Four of the ten RCTs reported the occurrence of adverse events, of which only one RCT indicated that patients had headache and sneezing while the remaining six did not.

Conclusions: This meta-analysis of 10 RCTs reveals that aromatherapy with single essential oil had a substantial effect on the sleep quality of cancer patients and should be recommended as a beneficial complementary therapy to promote sleep quality in cancer patients.
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http://dx.doi.org/10.1186/s12906-022-03668-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284915PMC
July 2022

A Novel Matrisomal-Related LncRNA Signature Associated With Survival Outcome and Immune Evasion in Patients With Gastric Cancer.

Front Oncol 2022 24;12:926404. Epub 2022 Jun 24.

Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China.

Background: Different matrisomal patterns are shared across carcinomas. However, little is known about whether there exists a unique tumor matrisome that modulates GC progression and immune regulation.

Methods: We conducted a genome-wide analysis based on matrisomal-related lncRNAs (MRLs) in 375 patients with GC from the Cancer Genome Atlas (TCGA) database. Patients were split into the training set and validation set at a ratio of 1:1 using the R package cart. Pearson correlation analysis (PCA) was performed to identify lncRNAs that correlated with matrisome based on differential expression genes. Subsequently, we performed univariate Cox regression analyses and lasso Cox analysis on these lncRNAs to construct a risk model. Considering the primary effect of GRASLND on the GC prognosis, we chose it for further validation in an experimental setting.

Results: We identified a 15-MRL signature to predict overall survival and immune cell infiltration of patients with GC. The AUC values to predict 5-year outcome in three sets were 0.89, 0.65, and 0.78, respectively. Further analyses suggested that the high-risk group showed more obvious immune cell infiltration, and demonstrated an immunologically "cold" profile. , knockdown of GRASLND could inhibit the invasion capability of GC cells, and downregulate the protein expression of crucial matrisomal-related gene MMP9.

Conclusions: The 15-MRL gene signature might serve as a relatively good predictive tool to manage patients with GC.
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http://dx.doi.org/10.3389/fonc.2022.926404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263572PMC
June 2022

Evolutionary Analysis of Four Recombinant Viruses of the Porcine Reproductive and Respiratory Syndrome Virus From a Pig Farm in China.

Front Vet Sci 2022 24;9:933896. Epub 2022 Jun 24.

College of Life Sciences, Longyan University, Longyan, China.

The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens causing substantial economic losses to the Chinese swine industry. In this study, we analyzed the complete genome sequences of four PRRSV isolates (PRRSV2/CN/SS0/2020, PRRSV2/CN/SS1/2021, PRRSV2/CN/L3/2021, and PRRSV2/CN/L4/2020) isolated from a single pig farm from 2020 to 2021. The genomes of the four isolates were 14,962-15,023 nt long, excluding the poly (A) tails. Comparative analysis of the genome sequences showed that the four isolates shared 93.2-98.1% homology and they had no close PRRSV relatives registered in the GenBank (<92%). Furthermore, PRRSV2/CN/SS0/2020 and PRRSV2/CN/SS1/2021 had characteristic 150-aa deletions (aa481+aa537-566 +aa628-747) that were identical to the live attenuated virus vaccine strain TJM-F92 (derived from the HP-PRRSV TJ). Further analysis of the full-length sequences suggests that the four isolates were natural recombinant strains between lineages 1 (NADC30-like), 3 (QYYZ-like), and 8.7 (JXA1-like). Animal experiments revealed discrepancies in virulence between PRRSV2/CN/SS0/2020 and PRRSV2/CN/L3/2021. The strain with high homology to HP-PRRSV demonstrates higher pathogenicity for pigs than the other isolate with low homology to HP-PRRSV. Taken together, our findings suggest that PRRSVs have undergone genome evolution by recombination among field strains/MLV-like strains of different lineages.
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http://dx.doi.org/10.3389/fvets.2022.933896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270021PMC
June 2022

Thrombocytopenia and increased risk of adverse outcome in COVID-19 patients.

PeerJ 2022 30;10:e13608. Epub 2022 Jun 30.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

Background: Thrombocytopenia was common in the coronavirus disease 2019 (COVID-19) patients during the infection, while the role of thrombocytopenia in COVID-19 pathogenesis and its relationship with systemic host response remained obscure. The study aimed to systematically evaluate the relationship between thrombocytopenia in COVID-19 patients and clinical, haematological and biochemical markers of the disease as well as adverse outcomes.

Methods: To assess the relationship between abnormal platelet levels and disease progression, a multi-center retrospective cohort study was conducted. COVID-19 patients with thrombocytopenia and a sub-cohort of matched patients without thrombocytopenia were compared for their clinical manifestations, haematological disorders, biochemical parameters, inflammatory markers and clinical outcome.

Results: Thrombocytopenia was present in 127 of 2,209 analyzed patients on admission. Compared with the control group, thrombocytopenia patients developed significantly higher frequency of respiratory failure (41.9% 22.6%,  = 0.020), intensive care unit entrance (25.6% 11.5%,  = 0.012), disseminated intravascular coagulation (45.2% 10.6%,  < 0.001), more altered platelet morphology indexes and coagulation perturbation, higher levels of inflammatory markers. In addition, a significantly increased all-cause mortality (hazard ratio 3.08, 95% confidence interval 2.26-4.18,  < 0.001) was also observed in the patients with thrombocytopenia. Late development of thrombocytopenia beyond 14 days post-symptom was observed in 61 patients, from whom a comparable mortality rate yet longer duration to death was observed compared to those with early thrombocytopenia.

Conclusions: Our finding from this study adds to previous evidence that thrombocytopenia is associated with adverse outcome of the disease and recommend that platelet count and indices be included alongside other haematological, biochemical and inflammatory markers in COVID-19 patients' assessment during the hospital stay.
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http://dx.doi.org/10.7717/peerj.13608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250762PMC
June 2022

Conductive Microneedle Patch with Electricity-Triggered Drug Release Performance for Atopic Dermatitis Treatment.

ACS Appl Mater Interfaces 2022 Jul 5;14(28):31645-31654. Epub 2022 Jul 5.

Beijing Laboratory of Biomedical Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Atopic dermatitis (AD) is a chronic inflammatory skin disease that seriously affects the life quality of patients. Topical administration of glucocorticoids is considered to be the most effective anti-inflammatory treatment. However, due to the barrier function of skin, only less than 20% of topical drug molecules could diffuse into the skin. Therefore, it is of great importance to develop an effective strategy to improve AD therapy. In this study, we reported a two-electrode microneedle patch (t-EMNP) composed of a polylactic acid-platinum (PLA-Pt) MN array and polylactic acid-platinum-polypyrrole (PLA-Pt-PPy) MN array for improving the transdermal drug delivery efficacy. The drug loading capability of MNs could be altered by employing different polymerization times and drug concentrations. The drug release rate of MNs could be changed by applying different voltages. We further developed a controlled transdermal drug delivery system (c-TDDS) based on this two-electrode microneedle patch (t-EMNP), exhibiting the remarkable performance of the electricity-triggered drug release profile. The drugs could be released with electrical stimulation, while there was almost no drug release without electrical stimulation. For AD treatment , this MN patch with electricity-triggered drug release performance could effectively deliver more drugs into the skin compared with other controls such as dexamethasone cream, which efficiently alleviate AD. In sum, this work not only developed a smart patch for improving AD treatment but also provided a promising approach of transdermal drug delivery on demand.
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http://dx.doi.org/10.1021/acsami.2c05952DOI Listing
July 2022

Reply to Kodner et al.: Fundamental misunderstanding of both model and methods.

Proc Natl Acad Sci U S A 2022 Jul 5;119(29):e2204944119. Epub 2022 Jul 5.

College of Computing, Georgia Institute of Technology, Atlanta, GA 30332.

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http://dx.doi.org/10.1073/pnas.2204944119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303956PMC
July 2022

TJP1, a Membrane-Expressed Protein, is a Potential Therapeutic and Prognostic Target for Lung Cancer.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221106855

School of Life Sciences, 12657Northwest University, Xi'an, China.

Lung cancer is a malignant tumor with the highest mortality rate in the world. It is necessary to develop effective biomarkers for diagnosis or prognostic treatment to improve the survival rate of patients. In this prospective study, we identified a membrane-expressed protein Tight Junction Protein 1 (TJP1), which is an ideal therapeutic target for lung cancer, and demonstrated its role in invasion, migration, and proliferation of lung cancer. High-throughput monoclonal antibody microarrays were used to screen for differential expression of monoclonal antibodies (mAbs) in lung cancer and normal lung tissue. Differentially expressed antibodies were used to immunoprecipitate their cellular targets to be identified by mass spectrometry. The identified target TJP1 was knocked down to observe the effect of reduced gene expression on lung cancer cell function. Immunohistochemistry on human tumor tissues and The Cancer Genome Atlas (TCGA) database was used to explore the relationship between TJP1 expression in multiple cancer types and patient prognosis. The antibody CL007473 was overexpressed in tumor tissue and its target protein was identified by mass spectrometry and immunofluorescence as TJP1, a membrane-expressed protein. Knockdown of TJP1 in lung cancer cell lines showed that reduced expression of TJP1 could inhibit the invasion and migration of lung cancer cells and inhibit the proliferation of cancer cells, suggesting that membrane-expressed protein TJP1 may be used as a therapeutic target for lung cancer. TCGA database analysis showed that TJP1 was highly expressed in pancreatic cancer (PAAD) tissues compared with normal tissues, and low expression was more beneficial to the prognosis and survival of PAAD patients. Membrane-expressed protein TJP1 may be a good therapeutic and prognostic target for lung cancer and has the potential to be a prognostic biomarker in pancreatic cancer.
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http://dx.doi.org/10.1177/15330338221106855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273941PMC
July 2022

Optimized structural parameters and heat extraction capacity of a mixing device for constant pressure CO mineralization using alkaline waste.

Environ Sci Pollut Res Int 2022 Jun 29. Epub 2022 Jun 29.

College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China.

Alkaline waste such as calcium carbide slag is an ideal material for mineralizing CO and promoting atmospheric carbon reduction. In this study, the structural parameters of a mixing device and a thermal extraction method for the high-efficiency mineralization of CO using alkaline waste were optimized. First, the influence of structural parameters was studied by means of numerical simulation, and it was found that when the length-diameter ratio, blade angle, spacing, and diameter of the mixing device were 3, 15, 6 cm, and 14 cm respectively, 2.14 t CO can be mineralized within 1 h. The amount of heat extracted from mineralization of 1 t CO reached 189.60 MJ. In addition, the winding configuration of the heat pipe, which is beneficial for extracting more reaction heat, was optimal, and a model of the relationship between the heat pipe outlet water temperature and flow velocity at the outlet of the heat pipe was established. This study provides theoretical guidance for the field application of alkaline waste for high-efficiency mineralization of CO, which can accelerate the realization of peak CO emissions and carbon neutrality.
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http://dx.doi.org/10.1007/s11356-022-21658-0DOI Listing
June 2022

Prevalence trends and risk factors associated with HIV, syphilis, and hepatitis C virus among pregnant women in Southwest China, 2009-2018.

AIDS Res Ther 2022 06 27;19(1):31. Epub 2022 Jun 27.

Guangxi Key Laboratory of AIDS Prevention and Treatment, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China.

Objective: This study investigated prevalence trends and identified the associated factors of HIV, syphilis and hepatitis C virus (HCV) among pregnant women in the Guangxi Zhuang Autonomous Region (Guangxi), Southwest China.

Methods: Serial cross-sectional surveys were performed annually among pregnant women in Guangxi from 2009 to 2018. Blood specimens were collected to test the prevalence of HIV, syphilis and HCV. Cochran-Armitage analysis was used to assess the trends of HIV, syphilis and HCV prevalence, as well as the sociodemographic and behavioural data. In this study, we used zero-inflated negative binomial (ZINB) regression models to identify factors associated with HIV, syphilis and HCV infection.

Results: A total of 23,879 pregnant women were included in the study. The prevalence of HIV, syphilis and HCV was 0.24%, 0.85% and 0.19%, respectively. There was a decrease in HIV prevalence from 0.54% to 0.10%, a decrease in HCV prevalence from 0.40% to 0.05% and a decrease in syphilis prevalence from 1.53% to 0.30%. The findings based on the ZINB model revealed that pregnant women who had a history of STI had significantly increased risks of HIV (OR 6.63; 95% CI 1.33-32.90) and syphilis (OR 9.06; 95% CI 3.85-21.30) infection, while pregnant women who were unmarried/widowed/divorced were more likely to have HIV (OR 2.81; 95% CI 1.20-6.54) and HCV (OR 58.12; 95% CI, 3.14-1076.99) infection. Furthermore, pregnant women whose husband had a history of STI (OR 5.62; 95% CI 1.24-25.38) or drug use (OR 7.36; 95% CI 1.25-43.43) showed an increased risk of HIV infection.

Conclusions: There was a relatively low prevalence of HIV, syphilis and HCV among pregnant women. Although decreasing trends in HIV, syphilis and HCV infections were observed, effort is needed to promote STI testing in both premarital medical check-ups and antenatal care, especially targeting couples with a history of STI or drug use.
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http://dx.doi.org/10.1186/s12981-022-00450-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238009PMC
June 2022

The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.

Pathol Res Pract 2022 Aug 17;236:153989. Epub 2022 Jun 17.

The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University (Naval Medical University), Shanghai 200438, China. Electronic address:

Tripartite motif containing 59 (TRIM59) is a crucial gene that is involved in the process of various types of cancer,including breast cancer, lung cancer, colorectal cancer,and so on. Its abnormal expression can affect tumor cell proliferation, metastasis, or apoptosis. In liver cancer, the incidence of intrahepatic cholangiocarcinoma (ICC) is increasing. However, However, it has not been clearly reported on TRIM59 affects the progress of intrahepatic cholangiocarcinoma cells.Firstly, we review the expression of TRIM59 in different cancers and the corresponding normal tissues,and the results preliminarily showed that TRIM59 may be abnormally expressed in many cancers. The author focuses on whether TRIM59 plays a crucial biological role in intrahepatic cholangiocarcinoma. Therefore, we have confirmed through online websites that TRIM59 is highly expressed in intrahepatic cholangiocarcinoma tissues. Furthermore we further found that TRIM59 can be used as an effective prognostic marker for the prognostic guidance of patient survival time. Next, we explore whether the expression level of TRIM59 in intrahepatic cholangiocarcinoma is related to proliferation through the CCK-8 and EDU assay in two ICC cell lines. To further explore how TRIM59 affected the molecular mechanism involved in intrahepatic cholangiocarcinoma cell growth, we found that STAT3 promotes TRIM59 transcription and TRIM59 can affect tumor progression by regulating the PI3K/AKT signaling pathway through luciferase reporter assay and Western blot experiments. In summary, we first found that TRIM59 has great research value in ICC through bioinformatic analysis, then its expression level is closely related to the prognosis through the analysis of clinicopathological indicators of patients with ICC, and the biological mechanism of TRIM59 in ICC provides precise research or therapeutic targets for future cancer treatment. The findings improve our understanding of the potential of TRIM59 in biological functions in ICC and may hold promise as markers for the diagnosis,treatment, and prognosis of ICC. DATA AVAILABILITY: The raw data of this study are derived from the TCGA database, which are publicly available databases.
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http://dx.doi.org/10.1016/j.prp.2022.153989DOI Listing
August 2022

A review: The detection of cancer cells in histopathology based on machine vision.

Comput Biol Med 2022 07 18;146:105636. Epub 2022 May 18.

Henan Key Lab of Intelligent Manufacturing of Mechanical Equipment, Zhengzhou University of Light Industry, Zhengzhou, 450002, China; Guangdong HUST Industrial Technology Research Institute, Guangdong Provincial Key Laboratory of Digital Manufacturing Equipment, Dongguan, 523808, China.

Machine vision is being employed in defect detection, size measurement, pattern recognition, image fusion, target tracking and 3D reconstruction. Traditional cancer detection methods are dominated by manual detection, which wastes time and manpower, and heavily relies on the pathologists' skill and work experience. Therefore, these manual detection approaches are not convenient for the inheritance of domain knowledge, and are not suitable for the rapid development of medical care in the future. The emergence of machine vision can iteratively update and learn the domain knowledge of cancer cell pathology detection to achieve automated, high-precision, and consistent detection. Consequently, this paper reviews the use of machine vision to detect cancer cells in histopathology images, as well as the benefits and drawbacks of various detection approaches. First, we review the application of image preprocessing and image segmentation in histopathology for the detection of cancer cells, and compare the benefits and drawbacks of different algorithms. Secondly, for the characteristics of histopathological cancer cell images, the research progress of shape, color and texture features and other methods is mainly reviewed. Furthermore, for the classification methods of histopathological cancer cell images, the benefits and drawbacks of traditional machine vision approaches and deep learning methods are compared and analyzed. Finally, the above research is discussed and forecasted, with the expected future development tendency serving as a guide for future research.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105636DOI Listing
July 2022
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