Publications by authors named "Yuan Xu"

887 Publications

Diagnostic Model for Discrimination Between Tuberculous Meningitis and Bacterial Meningitis.

Front Immunol 2021 12;12:731876. Epub 2021 Nov 12.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The differential diagnosis between tuberculous meningitis (TBM) and bacterial meningitis (BM) remains challenging in clinical practice. This study aimed to establish a diagnostic model that could accurately distinguish TBM from BM.

Methods: Patients with TBM or BM were recruited between January 2017 and January 2021 at Tongji Hospital (Qiaokou cohort) and Sino-French New City Hospital (Caidian cohort). The detection for indicators involved in cerebrospinal fluid (CSF) and T-SPOT assay were performed simultaneously. Multivariate logistic regression was used to create a diagnostic model.

Results: A total of 174 patients (76 TBM and 98 BM) and another 105 cases (39 TBM and 66 BM) were enrolled from Qiaokou cohort and Caidian cohort, respectively. Significantly higher level of CSF lymphocyte proportion while significantly lower levels of CSF chlorine, nucleated cell count, and neutrophil proportion were observed in TBM group when comparing with those in BM group. However, receiver operating characteristic (ROC) curve analysis showed that the areas under the ROC curve (AUCs) produced by these indicators were all under 0.8. Meanwhile, tuberculosis-specific antigen/phytohemagglutinin (TBAg/PHA) ratio yielded an AUC of 0.889 (95% CI, 0.840-0.938) in distinguishing TBM from BM, with a sensitivity of 68.42% (95% CI, 57.30%-77.77%) and a specificity of 92.86% (95% CI, 85.98%-96.50%) when a cutoff value of 0.163 was used. Consequently, we successfully established a diagnostic model based on the combination of TBAg/PHA ratio, CSF chlorine, CSF nucleated cell count, and CSF lymphocyte proportion for discrimination between TBM and BM. The established model showed good performance in differentiating TBM from BM (AUC: 0.949; 95% CI, 0.921-0.978), with 81.58% (95% CI, 71.42%-88.70%) sensitivity and 91.84% (95% CI, 84.71%-95.81%) specificity. The performance of the diagnostic model obtained in Qiaokou cohort was further validated in Caidian cohort. The diagnostic model in Caidian cohort produced an AUC of 0.923 (95% CI, 0.867-0.980) with 79.49% (95% CI, 64.47%-89.22%) sensitivity and 90.91% (95% CI, 81.55%-95.77%) specificity.

Conclusions: The diagnostic model established based on the combination of four indicators had excellent utility in the discrimination between TBM and BM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.731876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632769PMC
November 2021

Utilizing reclassification to explore characteristics and prognosis of KDIGO AKI subgroups: a retrospective analysis of a multicenter prospective cohort study.

Ren Fail 2021 Dec;43(1):1569-1576

Medical Intensive Care Unit, Peking Union Medical College Hospital, Beijing, China.

Background: Acute kidney injury (AKI) is widespread in the intensive care unit (ICU) and affects patient prognosis. According to Kidney Disease: Improving Global Outcomes (KDIGO) guidelines, the absolute and relative increases of serum creatinine (Scr) are classified into the same stage. Whether the prognosis of the two types of patients is similar in the ICU remains unclear.

Methods: According to the absolute and relative increase of Scr, AKI stage 1 and stage 3 patients were divided into stage 1a and 1b, stage 3a and 3b groups, respectively. Their demographics, laboratory results, clinical characteristics, and outcomes were analyzed retrospectively.

Results: Of the 345 eligible cases, we analyzed stage 1 because stage 3a group had only one patient. Using 53 or 61.88 µmol/L as the reference Scr (Scr), no significant differences were observed in ICU mortality (=0.076, =0.070) or renal replacement therapy (RRT) ratio, (=0.356, =0.471) between stage 1a and 1b, but stage 1b had longer ICU length of stay (LOS) than stage 1a (<0.001, =0.032). In the Kaplan-Meier survival analysis, no differences were observed in ICU mortality between stage 1a and 1b (=0.378, =0.255). In a multivariate analysis, respiratory failure [HR = 4.462 (95% CI 1.144-17.401),  = 0.031] and vasoactive drug therapy [HR = 4.023 (95% CI 1.584-10.216),  = 0.003] were found to be independently associated with increased risk of death.

Conclusion: ICU LOS benefit was more prominent in KDIGO AKI stage 1a patients than in stage 1 b. Further prospective studies with a larger sample size are necessary to confirm the effectiveness of reclassification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/0886022X.2021.1997761DOI Listing
December 2021

Risk assessment of the inhibition of hydroxygenkwanin on human and rat cytochrome P450 by cocktail method.

Toxicol In Vitro 2021 Nov 26:105281. Epub 2021 Nov 26.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Hydroxygenkwanin (HGK), a natural flavonoid extracted from the buds of Daphne genkwa Sieb.et Zucc. (Thymelaeaceae), possesses a wide range of pharmacological activities, including anti-inflammatory, antibacterial and anticancer. However, the inhibitory effect of HGK on cytochrome P450 (CYP) remains unclear. This study investigated the potential inhibitory effects of HGK on CYP1A2, 2B1/6, 2C9/11, 2D1/6, 2E1 and 3A2/4 enzymes in human and rat liver microsomes (HLMs and RLMs) by the cocktail approach. HGK exhibited no time-dependent inhibition of CYP activities in HLMs and RLMs. Enzyme inhibition kinetics indicated that HGK was not only a competitive inhibitor of human CYP1A2 and 2C9, but also competitively inhibited rat CYP1A2 and 2C11 activities, with K value at 0.84 ± 0.03, 8.09 ± 0.44, 2.68 ± 0.32 and 8.35 ± 0.31 μM, respectively. Further study showed inhibitory effect of HGK on CYP enzymes was weaker than that of diosmetin, which may be related to the substitution of hydroxyl and methoxy in the A and B rings of the flavone skeleton. Therefore, the low K values of HGK for CYP1A2 and 2C may lead to potential drug-drug interactions and toxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tiv.2021.105281DOI Listing
November 2021

Fully Vacuum-Sealed Diode-Structure Addressable ZnO Nanowire Cold Cathode Flat-Panel X-ray Source: Fabrication and Imaging Application.

Nanomaterials (Basel) 2021 Nov 18;11(11). Epub 2021 Nov 18.

State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-Sen University, Guangzhou 510275, China.

A fully vacuum-sealed addressable flat-panel X-ray source based on ZnO nanowire field emitter arrays (FEAs) was fabricated. The device has a diode structure composed of cathode panel and anode panel. ZnO nanowire cold cathodes were prepared on strip electrodes on a cathode panel and Mo thin film strips were prepared on an anode panel acting as the target. Localized X-ray emission was realized by cross-addressing of cathode and anode electrodes. A radiation dose rate of 10.8 μGy/s was recorded at the anode voltage of 32 kV. The X-ray imaging of objects using different addressing scheme was obtained and the imaging results were analyzed. The results demonstrated the feasibility of achieving addressable flat-panel X-ray source using diode-structure for advanced X-ray imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11113115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624030PMC
November 2021

Non-coplanar volumetric modulated arc therapy for locoregional radiotherapy of left-sided breast cancer including internal mammary nodes.

Radiol Oncol 2021 11 19;55(4):499-507. Epub 2021 Nov 19.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Non-coplanar volumetric modulated arc therapy (ncVMAT) is proposed to reduce toxicity in heart and lungs for locoregional radiotherapy of left-sided breast cancer, including internal mammary nodes (IMN).

Patients And Methods: This retrospective study included 10 patients with left-sided breast cancer who underwent locoregional radiotherapy after breast-conserving surgery. For each patient, the ncVMAT plan was designed with four partial arcs comprising two coplanar arcs and two non-coplanar arcs, with a couch rotating to 90°. The prescribed dose was normalized to cover 95% of planning target volume (PTV), with 50 Gy delivered in 25 fractions. For each ncVMAT plan, dosimetric parameters were compared with the coplanar volumetric modulated arc therapy (coV-MAT) plan.

Results: T here were improvements in conformity index, homogeneity index and V of total target volume (PTVall) comparing ncVMAT to coVMAT ( < 0.001). Among the organs at risk, the average V, V, V, V, and mean dose (D) of the heart decreased significantly ( < 0.001). Furthermore, ncVMAT significantly reduced the mean V, V, V, and D of left lung and the mean V and V and D of contralateral lung ( < 0.001). An improved sparing of the left anterior descending coronary artery and right breast were also observed with ncVMAT ( < 0.001).

Conclusions: Compared to coVMAT, ncVMAT provides improved conformity and homogeneity of whole P TV, better dose sparing of the heart, bilateral lungs, left anterior descending coronary artery (LAD), and right breast for locoregional radiotherapy of left-sided breast cancer with IMN, potentially reducing the risk of normal tissue damage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2478/raon-2021-0045DOI Listing
November 2021

Neural Adaptive Fixed-Time Control for Nonlinear Systems With Full-State Constraints.

IEEE Trans Cybern 2021 Nov 18;PP. Epub 2021 Nov 18.

This article aims at this problem of adaptive neural tracking control for state-constrained systems. A general fixed-time stability criterion is first presented, by which an adaptive neural control algorithm is developed. Under the action of the proposed adaptive neural tracking controller, the tracking error converges into a small neighborhood around the origin in fixed time; meanwhile, all system states abide by the corresponding state constraints for all the time. The main difference between the present research and the previous control schemes for state-constrained systems is that this article proposes a novel and feasible approach to ensure that the constructed virtual control signals satisfy the state constraints on the corresponding states viewed as the virtual control inputs. Such an approach guarantees theoretically that all the system states cannot violate their constrained requirements at any time. Finally, two simulation examples provide support to the proposed results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCYB.2021.3125678DOI Listing
November 2021

The IVF-generated human embryonic microenvironment reverses progestin resistance in endometrial cancer cells by inducing cancer stem cell differentiation.

Cancer Lett 2021 Nov 12. Epub 2021 Nov 12.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Renji Hospital, Shanghai Jiao Tong University, Shanghai, 200135, China. Electronic address:

Progestin resistance is a critical factor that prevents patients with endometrial cancer (EC) from receiving conservative therapy. However, the etiology remains elusive. Cancer stem cells (CSCs) may be a contributing factor to progestin resistance in EC. These cells share similar stemness properties with embryonic stem cells that have a multipotent but differential naïve phenotype. Embryonic stem cells are programed to self-renew, to differentiate and to show plasticity toward a normal cellular phenotype in their defined microenvironment. However, whether this microenvironment may promote CSC differentiation toward a better responsive phenotype and reverse progestin resistance has not yet been clarified. In the current study, we found that progestin resistance of endometrial CSCs can be improved or reversed by using in vitro fertilization (IVF)-generated embryonic sac-derived fluid containing the embryonic microenvironment. Furthermore, suppression or reversal of progestin resistance was mediated by placental alkaline phosphatase (ALPP), a factor secreted into the embryonic microenvironment by IVF-generated blastocysts. ALPP significantly reversed progestin resistance by facilitating endometrial CSC differentiation through downregulating the stemness genes NANOG, OCT4 and SOX2. We further showed that the downregulation of NANOG, OCT4 and SOX2 by ALPP was carried out by TET1/2-mediated epigenetic modulation of the promoter regions of these genes. Such changes at the molecular level initiated endometrial CSC differentiation and promoted a better responsive endometrial cancer phenotype. In fact, their response to progestin treatment was similar to that of well-differentiated endometrioid carcinoma cells without CSCs. ALPP could be a novel target in the process to overcome progestin resistance, and such findings may provide a new approach for the conservative treatment of endometrial cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2021.11.003DOI Listing
November 2021

First Report of Powdery Mildew Caused By on Scarlet Beebalm () in China.

Plant Dis 2021 Nov 11. Epub 2021 Nov 11.

Shangqiu Normal University, 117757, Life Science, 289, Wenhua Rd., Shangqiu, Henan, China, 476000;

Scarlet Beebalm (Monarda didyma) is a perennial ornamental flowering plant in the mint family, Labiatae. Due to low-maintenance, and a long blooming period, it is commonly cultivated in gardens as an ornamental plant in China. In May 2021, a disease was observed on the leaves of a capitals beebalm plant in a Ten Mile Flower Sea in Xiayi county (116°13'8″E, 34°14'45″N), Henan province of China. Symptoms first appeared as nearly circular, small, white, powdery mildew-like spots on the leaves which gradually expand, covering the entire leaves. The lesions spread from the lower leaves to the upper canopy, and the stems were also covered by white mycelia. In severe cases, early defoliation occured. About 30% plants were affected. Representative voucher specimens (SQNUMd04, SQNUDn4) were deposited in the herbarium of Shangqiu Normal University (SQNU), Shangqiu, China. Conidiophores (n = 30) were cylindrical, 92.0 to 142.2 µm long and 10.8 to 14.2 µm wide, and produced 5 to 7 immature conidia in a chain. Foot-cells of conidiophores were mostly curved at the base. Conidia (n = 30) were hyaline, ellipsoid, 23.3 to 29.8 μm (avg. 26.6 μm) long, and 11.2 to 16.9 μm (avg. 14.5μm) width, a length/width ratio of 1.5 to 2.1, and germ tubes were produced at the perihilar position. No chasmothecia were observed. Based on morphological comparison using the description by Scholler et al. (2016) description the fungus was tentatively identified as Golovinomyces monardae (G.S. Nagy) M. Scholler, U. Braun & Anke Schmidt. For molecular identification, DNA was extracted from mycelia and conidia, which were collected by scraping symptomatic leaves.The ITS regions and LSU were amplified using primers ITS1/ITS4 (White et al. 1990) and NL1/NL4 (Horisawa et al. 2013). BLASTn analysis of the (MZ303741) and LSU (MZ305434) sequences showed 100% identity with those of G. monardae (AB307667, LC076800, LC076802, LC076808, and AB077691) reported on Monarda species (Matsuda et al. 2003; Takamatsu et al. 2013; Scholler et al. 2016). Pathogenicity tests were carried out by gently dusting conidia from infected leaves onto healthy leaves of five M. didyma plants and five non-inoculated plants used as controls. After 9 days, typical powdery mildew colonies started to appear on the inoculated leaves while control plants remained disease free. All plants were placed in a greenhouse without temperature and humidity control. Based on morphology, fungus was identified as the same as that used for inoculum, fulfilling Koch's postulates. Although G. monardae has been reported on various genera in the Labiatae and Verbenaceae (Farr and Rossman 2021), to our knowledge, this is the first report of G. monardae causing powdery mildew of Scarlet Beebalm(M. didyma) in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-08-21-1803-PDNDOI Listing
November 2021

Rabbit microbiota across the whole body revealed by 16S rRNA gene amplicon sequencing.

BMC Microbiol 2021 11 10;21(1):312. Epub 2021 Nov 10.

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.

Background: Rabbit can produce meat, fur and leather, and serves as an important biomedical animal model. Understanding the microbial community of rabbits helps to raise rabbits healthily and better support their application as animal models.

Results: In this study, we selected 4 healthy Belgium gray rabbits to collect the microbial samples from 12 body sites, including skin, lung, uterus, mouth, stomach, duodenum, ileum, jejunum, colon, cecum, cecal appendix and rectum. The microbiota across rabbit whole body was investigated via 16S rRNA gene amplicon sequencing. After quality control, 46 samples were retained, and 3,148 qualified ASVs were obtained, representing 23 phyla and 264 genera. Based on the weighted UniFrac distances, these samples were divided into the large intestine (Lin), stomach and small intestine (SSin), uterus (Uter), and skin, mouth and lung (SML) groups. The diversity of Lin microbiota was the highest, followed by those of the SSin, Uter and SML groups. In the whole body, Firmicutes (62.37%), Proteobacteria (13.44%) and Bacteroidota (11.84%) were the most predominant phyla. The relative abundance of Firmicutes in the intestinal tract was significantly higher than that in the non-intestinal site, while Proteobacteria was significantly higher in the non-intestinal site. Among the 264 genera, 35 were the core microbiota distributed in all body sites. Sixty-one genera were specific in the SML group, while 13, 8 and 1 were specifically found in the Lin, SSin and Uter groups, respectively. The Lin group had the most difference with other groups, there were average 72 differential genera between the Lin and other groups. The functional prediction analysis showed that microbial function within each group was similar, but there was a big difference between the intestinal tracts and the non-intestinal group. Notably, the function of microorganism in uterus and mouth were the most different from those in the gastrointestinal sites; rabbit's coprophagy of consuming soft feces possibly resulted in little differences of microbial function between stomach and large intestinal sites.

Conclusion: Our findings improve the knowledge about rabbit microbial communities throughout whole body and give insights into the relationship of microbial communities among different body sites in health rabbits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12866-021-02377-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579649PMC
November 2021

Cascaded Nanozyme System with High Reaction Selectivity by Substrate Screening and Channeling in a Microfluidic Device*.

Angew Chem Int Ed Engl 2021 Nov 8. Epub 2021 Nov 8.

Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Medical School, Southeast University, Nanjing, 211189, China.

Surpassing natural enzymes in cost, stability and mass production, nanozymes have attracted wide attention in fields from disease diagnosis to tumor therapy. However, nanozymes intrinsically have low reaction selectivity, which significantly restricts their applications. A general method is reported to address this challenge by following a biomimetic operation principle of substrates channeling and screening. Two oxidase- and peroxidase-like nanozymes (i.e., emerging N-doped carbon nanocages and Prussian blue nanoparticles), were cascaded as a proof of concept to improve the reaction selectivity in transforming the substrate into the targeted product by more than 2000 times. The cascaded nanozymes were also adopted to a spatially confined microfluidic device, leading to more than 100-fold enhancement of the reaction efficiency due to signal amplification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202112453DOI Listing
November 2021

Associations Between the Density of Oil and Gas Infrastructure and the Incidence, Stage and Outcomes of Solid Tumours: A Population-Based Geographic Analysis.

Front Oncol 2021 15;11:757875. Epub 2021 Oct 15.

Department of Surgery, University of Calgary, Calgary, AB, Canada.

Background: We hypothesized that there are geographic areas of increased cancer incidence in Alberta, and that these are associated with high densities of oil and gas(O+G) infrastructure. Our objective was to describe the relationship between O+G infrastructure and incidence of solid tumours on a population level.

Methods: We analyzed all patients >=18 years old with urological, breast, upper GI, colorectal, head and neck, hepatobiliary, lung, melanoma, and prostate cancers identified from the Alberta Cancer Registry from 2004-2016. Locations of active and orphan O+G sites were obtained from the Alberta Energy Regulator and Orphan Well Association. Orphan sites have no entity responsible for their maintenance. ArcGIS (ESRI, Toronto, Ontario) was used to calculate the distribution of O+G sites in each census distribution area (DA). Patient residence at diagnosis was defined by postal code. Incidence of cancer per DA was calculated and standardized. Negative binomial regression was done on O+G site density as a categorical variable with cutoffs of 1 and 30 wells/100km, compared to areas with 0 sites.

Results: 125,316 patients were identified in the study timeframe;58,243 (46.5%) were female, mean age 65.6 years. Breast (22%) and prostate (19.8%) cancers were most common. Mortality was 36.5% after a median of 30 months follow up (IQR 8.4 - 68.4). For categorical density of active O+G sites, RR was 1.02 for 1-30 sites/100km (95% CI=0.95-1.11) and 1.15 for >30 sites/100km2 (p<0.0001, 95%CI=1.11-1.2). For orphan sites, 1-30 sites RR was 1.25 (p<0.0001, 95%CI=1.16-1.36) and 1.01 (p=0.97, 95%CI=0.7-1.45) for >30 sites. For all O+G sites, RR for 1-30 sites was 1.03 (p=0.4328, 95%CI=0.95-1.11) and 1.15 (p<0.0001, 95%CI=1.11-1.2) for >30 sites.

Conclusion: We report a statistically significant correlation between O+G infrastructure density and solid tumour incidence in Alberta. To our knowledge this is the first population-level study to observe that active and orphan O+G sites are associated with increased risk of solid tumours. This finding may inform policy on remediation and cancer prevention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.757875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555261PMC
October 2021

Emerging role of TWEAK-Fn14 axis in lupus, a disease related to autoimmunity and fibrosis.

Int J Rheum Dis 2021 Oct 29. Epub 2021 Oct 29.

School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder. Lupus nephritis (LN) is one of the severe clinical implications in SLE, and this was relates to fibrosis in the kidney. As an important marker in the tumor necrosis factor (TNF) superfamily, TNF-like weak inducer of apoptosis (TWEAK) has been given much attention with respect to its role in regulating pro-inflammatory immune response. Fibroblast growth factor-inducible 14 (Fn14), the sole receptor for TWEAK, has been found expressed in different immune and non-immune cells. TWEAK binds to Fn14, and then regulates inflammatory components production via downstream signaling pathways. To date, dysregulated expression of TWEAK, Fn14 has been reported in SLE, LN patients, and in vivo, in vitro studies have discussed the significant role of TWEAK-Fn14 axis in SLE, LN pathogenesis, partly through mediating the fibrosis process. In this review, we will discuss the association of TWEAK-Fn14 axis in lupus. Understanding the relationship will better realize the potential for making TWEAK-Fn14 as a marker for the diseases, and will help to give many clues for targeting them in treatment of lupus in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1756-185X.14232DOI Listing
October 2021

[Free trajectory cone beam computed tomography reconstruction method for synchronous scanning of geometric calibration phantom and imaging object].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Oct;38(5):951-959

School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, P.R.China.

In order to suppress the geometrical artifacts caused by random jitter in ray source scanning, and to achieve flexible ray source scanning trajectory and meet the requirements of task-driven scanning imaging, a method of free trajectory cone-beam computed tomography (CBCT) reconstruction is proposed in this paper. This method proposed a geometric calibration method of two-dimensional plane. Based on this method, the geometric calibration phantom and the imaging object could be simultaneously imaged. Then, the geometric parameters could be obtained by online calibration method, and then combined with the geometric parameters, the alternating direction multiplier method (ADMM) was used for image iterative reconstruction. Experimental results showed that this method obtained high quality reconstruction image with high contrast and clear feature edge. The root mean square errors (RMSE) of the simulation results were rather small, and the structural similarity (SSIM) values were all above 0.99. The experimental results showed that it had lower image information entropy (IE) and higher contrast noise ratio (CNR). This method provides some practical value for CBCT to realize trajectory freedom and obtain high quality reconstructed image.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1001-5515.202101066DOI Listing
October 2021

Levosimendan to Facilitate Weaning From Cardiorespiratory Support in Critically Ill Patients: A Meta-Analysis.

Front Med (Lausanne) 2021 12;8:741108. Epub 2021 Oct 12.

Department of Critical Care Medicine, School of Clinical Medicine, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, China.

Cardiopulmonary support, as extracorporeal membrane oxygenation (ECMO) or mechanical ventilation (MV), is crucial for ICU patients. However, some of these patients are difficult to wean. Therefore, we aimed to assess the efficacy and safety of levosimendan in facilitating weaning from cardiorespiratory support in this patient population. We searched for potentially relevant articles in PubMed, Embase, China National Knowledge Infrastructure, Wanfang, and the Cochrane database from inception up to Feb 30, 2021. Studies focusing on weaning data in MV/ECMO adult patients who received levosimendan compared to controls were included. We used the Cochrane risk of bias tool or the Newcastle-Ottawa Quality Assessment Scale to evaluate the study quality. The primary outcome was the weaning rate from MV/ECMO. Secondary outcomes were mortality, duration of MV, and ICU stay. Subgroup analysis, sensitivity analysis, and publication bias were also conducted. Eighteen studies with 2,274 patients were included. The quality of the included studies was low to moderate. Overall, levosimendan effectively improved weaning rates from MV/ECMO [odds ratio (OR) = 2.32; 95%CI, 1.60-3.36; < 0.00001, = 68%]. Subgroup analyses confirmed the higher successful weaning rates in ventilated patients with low left ventricular ejection fractions (OR = 4.06; 95%CI, 2.16-7.62), patients with ECMO after cardiac surgery (OR = 2.04; 95%CI, 1.25-3.34), and patients with ECMO and cardiogenic shock (OR = 1.98; 95%CI, 1.34-2.91). However, levosimendan showed no beneficial effect on patients with MV weaning difficulty (OR = 2.28; 95%CI, 0.72-7.25). Additionally, no differences were found concerning the secondary outcomes between the groups. Levosimendan therapy significantly increased successful weaning rates in patients with cardiopulmonary support, especially patients with combined cardiac insufficiency. Large-scale, well-designed RCTs will be needed to define the subgroup of patients most likely to benefit from this strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.741108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546177PMC
October 2021

Chromosome-Level Genome Assembly of the Asian Red-Tail Catfish ().

Front Genet 2021 12;12:747684. Epub 2021 Oct 12.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.747684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546334PMC
October 2021

Phthalide and 1-Iodooctadecane Synergistic Optimization for Highly Efficient and Stable Perovskite Solar Cells.

Small 2021 Oct 27:e2103336. Epub 2021 Oct 27.

Engineering Research Center of Environment-Friendly Functional Materials, Ministry of Education, Institute of Materials Phys. Chem., Huaqiao Univ., Xiamen, 361021, China.

The carrier non-radiative recombination and instability of device caused by the inherent defects are main factors limiting development of perovskite solar cells (PSCs). During the fabrication process of a PSC device, perovskite films often produce Pb and I defects. This paper reports a strategy for synergistic optimization of perovskite films by defects passivation and surface modification. The doping of phthalide (PT) in the Pb-rich (CH(NH ) ) (CH NH ) PbI film can passivate lead cation defects, and the modification of 1-iodooctadecane (1-IO) can reduce halogen anion defects and improve stability of PSCs owing to its hydrophobicity. The PT and 1-IO optimized device achieves a power conversion efficiency (PCE) of 22.27%. The optimized PSCs remain 93.2% of the initial PCE when placed in air environment (relative humidity of 10%, 25 °C) more than 70 days. The PT and 1-IO synergistic optimization provides a novel strategy for improving the performance and stability of PSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202103336DOI Listing
October 2021

Comprehensive Analysis of Aquaporin Superfamily in Lung Adenocarcinoma.

Front Mol Biosci 2021 11;8:736367. Epub 2021 Oct 11.

Department of Respiratory Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.

Lung adenocarcinoma (LUAD) is the most predomintnt lung cancer subtype with increasing morbidity and mortality. Previous studies have shown that aquaporin (AQP) family genes were correlated with tumor progression and metastasis in several kinds of malignancies. However, their biological behaviors and prognostic values in LUAD have not been comprehensively elucidated. RNA sequencing and real-time reverse transcription PCR (RT-PCR) were used to assess AQP1/3/4/5 gene expressions in LUAD patients using GEPIA and UALCAN databases. And then Kaplan-Meier analysis, cBioPortal, Metascape, GeneMANIA, TISIDB, and TIMER were utilized to determine the prognostic value, mutation frequency, and immune cell infiltration of AQP family members in LUAD. We found that AQP3 expression was significantly elevated and AQP1 expression was markedly reduced in LUAD patients, whereas the expression levels of AQP4 and AQP5 exhibited no significant changes. The Kaplan-Meier survival analysis indicated that the higher expressions of AQP1/4/5 were related to longer overall survival (OS). Of interest, AQP3 was significantly correlated with the clinical tumor stage and lower AQP3 expression showed favorable prognosis in stage I LUAD patients, which indicated that AQP3 may be a potential prognostic biomarker for patients. Through functional enrichment analysis, the functions of these four AQPs genes were mainly involved in the passive transport by aquaporins, water homeostasis, and protein tetramerization. Moreover, AQP1/3/4/5 expression was strongly associated with tumor-infiltrating lymphocytes (TILs) in LUAD. AQP3 can be used as a prognosis and survival biomarker for stage I LUAD. These findings may provide novel insights into developing molecular targeted therapies in LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmolb.2021.736367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542973PMC
October 2021

Chemical characteristics, evolution, and quality of groundwater and processes controlling its fluoride concentration features: case study of a typical high-fluoride areas in the Southwestern Shandong Plain, China.

Environ Sci Pollut Res Int 2021 Oct 27. Epub 2021 Oct 27.

801 Institute of Hydrogeology and Engineering Geology, Shandong Provincial Bureau of Geology & Mineral Resources, Jinan, 250014, China.

In this study, the groundwater (GW) in the high-fluorine area of the Southwestern Shandong Plain was divided according to the characteristics of high Na% (> 75%), and its water chemistry characteristics and causes were discussed separately, and the hydrochemical process of the formation of high-fluorine GW was determined. Finally, the GW quality of the study area was evaluated. The results proved that silicate hydrolysis can significantly promote the release of F in fluorine-containing minerals; high %Na can be used as one of the early-warning conditions for judging high-fluoride areas. To this end, 132 GW samples were collected from 66 wells during the dry and wet seasons. The study area was found to have weakly alkaline GW (pH 7.1-8.9) and could be divided into high %Na areas (HNA) and non-HNA. GW exhibited different hydrochemical characteristics between HNA and non-HNA. In non-HNA, total hardness (TH) exceeded 200 mg/L, and total dissolved solids (TDS) ranged from 514.1 to 5246.1 mg/L; in HNA, TH was less than 200 mg/L, TDS ranged from 552.8 to 1298.3 mg/L, and Na increased with TDS, whereas Ca and Mg contents were low. The main water type in HNA was HCO-Na and in non-HNA was SO·Cl-Ca·Mg and SO·Cl-Na. The study area is experiencing serious fluoride pollution. GW in HNA is mostly controlled by carbonate and silicate hydrolysis and evaporation, whereas GW in non-HNA is controlled by dolomite dissolution and cation exchange in the main. Moreover, GW in HNA has significantly been altered by albite hydrolysis, which produces Na and HCO and triggers various reactions promoting the release of F from fluorine-containing minerals (FCM). Regarding the water quality for irrigation, GW in HNA was found to be less suitable than that in non-HNA. Nevertheless, in terms of the water quality index (WQI), GW is moderate for drinking and poor for irrigation. Therefore, extensive attention should be paid to the exploitation and management of high-sodium GW in the plain area.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-16928-2DOI Listing
October 2021

Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain.

Evid Based Complement Alternat Med 2021 12;2021:5528780. Epub 2021 Oct 12.

Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Background: Manual acupuncture (MA) with different stimulus frequencies may give rise to varying acupuncture effects. However, the intensity-effect relationship and the underlying mechanisms of MA remain unclear.

Objective: To compare the analgesic effects of different frequencies of twirling-rotating MA on rats with complete Freund's adjuvant- (CFA-) induced pain and explore the underlying mechanism via peripheral sensory nerves.

Methods: First, 36 healthy male Wistar rats were randomly divided into 6 groups: control group, 2 r/s MA group (twirling-rotating MA with the frequency of 2 revolutions per second), 4 r/s MA group (twirling-rotating MA with the frequency of 4 revolutions per second), CFA group, CFA + 2 r/s MA group, and CFA + 4 r/s MA group. Rats in three CFA groups received an intraplantar injection of CFA to establish a pain model, while the rats in other three groups received an intraplantar injection of saline. Rats in the 2 r/s MA group and 4 r/s MA group were treated with the corresponding frequencies of twirling-rotating MA on bilateral Zusanli (ST36) and Kunlun (BL60) for 7 days. The ipsilateral nociceptive thresholds (paw withdrawal latency; PWL) were tested to evaluate the analgesic effects. Second, 9 healthy male Wistar rats were randomly divided into 3 groups: control group, 2 r/s MA group, and 4 r/s MA group. The proportion of C-fiber neurons (calcitonin gene-related peptide- (CGRP-) positive neurons) and A-fiber neurons (neurofilament 200- (NF200-) positive neurons) in the dorsal root ganglia (DRG) activated by MA were quantitatively analyzed with the morphological immunofluorescence staining method. Third, 30 healthy male Wistar rats were randomly divided into 6 groups: control group, CFA group, CFA + 2 r/s MA group, CFA + 2 r/s MA + RTX group, CFA + 4 r/s MA group, and CFA  + 4 r/s MA + RTX group. Resiniferatoxin (RTX) was injected into the acupoints before acupuncture. PWL was evaluated to investigate the analgesic effect.

Results: Both types of MA treatment increased the PWL of saline-injecting rats and pain model rats. Moreover, 4 r/s MA was superior to 2 r/s MA in increasing PWL. A higher quantity of excited C-fiber neurons was observed following 4 r/s MA than 2 r/s MA, while the reverse was observed in the activation of A-fiber neurons. Following the injection of RTX to inhibit the activation of C-fibers, the analgesic effect of 4 r/s MA reduced significantly but not of 2 r/s MA.

Conclusion: Strong MA (4 r/s MA) has superior analgesic effects to gentle MA (2 r/s MA) on CFA model rats, which is associated with C-fiber activation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5528780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526210PMC
October 2021

Multiple attribute group decision-making based on interval-valued q-rung orthopair uncertain linguistic power Muirhead mean operators and linguistic scale functions.

PLoS One 2021 21;16(10):e0258772. Epub 2021 Oct 21.

School of Economics and Management, Beijing University of Chemical Technology, Beijing, China.

Fuzzy set theory and its extended form have been widely used in multiple-attribute group decision-making (MAGDM) problems, among which the interval-valued q-rung orthopair fuzzy sets (IVq-ROFSs) got a lot of attention for its ability of capturing information denoted by interval values. Based on the previous studies, to find a better solution for fusing qualitative quantization information with fuzzy numbers, we propose a novel definition of interval-valued q-rung orthopair uncertain linguistic sets (IVq-ROULSs) based on the linguistic scale functions, as well as its corresponding properties, such as operational rules and the comparison method. Furthermore, we utilize the power Muirhead mean operators to construct the information fusion method, and provide a variety of aggregation operators based on the proposed information description environment. A model framework is constructed for solving the MAGDM problem utilizing the proposed method. Finally, we illustrate the performance of the new method and investigate its advantages and superiorities through comparative analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258772PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530323PMC
November 2021

Microglia polarization in ischemic stroke: complex mechanisms and therapeutic interventions.

Chin Med J (Engl) 2021 Sep 15;134(20):2415-2417. Epub 2021 Sep 15.

Department of Neurosurgery, The First People's Hospital of Changzhou, Changzhou, Jiangsu 213003, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001711DOI Listing
September 2021

A longitudinal study of cardiac structure and function using echocardiography in patients undergoing peritoneal dialysis.

BMC Nephrol 2021 Oct 16;22(1):342. Epub 2021 Oct 16.

Department of Nephrology, The Second Affiliated Hospital of Guangzhou, University of Chinese Medicine, No.111 Dade Road, 510120, Guangzhou, China.

Background: Peritoneal dialysis (PD) can be associated with abnormal cardiac structure and function and increased mortality risk. Therefore, in this study, we analyzed the cardiac structure and function dynamic changes using echocardiography during the first 2 years of PD therapy. We also assessed its associations with all-cause mortality risk after 2 years of follow-up.

Methods: End-stage renal disease (ESRD) patients that have started PD from 2011 to 2017, and had echocardiography at baseline and years 1 and 2, were included in this study. Echocardiographic parameters were compared between baseline and year 2. Multivariable Cox models were used to estimate the association between echocardiographic parameters changes and all-cause mortality risk.

Results: We finally enrolled 72 PD patients in this study. The mean right ventricular diameter (RVD) increased from baseline (18.31 mm) to year 1 (18.75 mm) and year 2 (19.65 mm). We also observed a significant decrease in cardiac output (CO) between baseline and year 2. Additionally, a slight decrease trend in ejection fraction (EF) was observed. Finally, every 1 % increase in RVD was associated with a 68.2 % higher mortality risk after dialysis (HR, 1.682; 95 % CI, 1.017-2.783).

Conclusions: Our results demonstrated a susceptibility for deteriorated right cardiac structure and function during the first 2 years of PD treatment. Also, higher all-cause mortality risk was observed after 2 years of PD. Altogether, these results highlighted the need for additional focus on regular echocardiographic examinations during long-term PD management.

Trial Registration: The PD-CRISC cohort, registered with the Chinese Clinical Trial Registry ( ChiCTR1900023565 ).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12882-021-02535-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520263PMC
October 2021

Photodynamic Therapy with Tumor Cell Discrimination through RNA-Targeting Ability of Photosensitizer.

Molecules 2021 Oct 2;26(19). Epub 2021 Oct 2.

School of Biological Engineering, Dalian Polytechnic University, Dalian 116023, China.

Photodynamic therapy (PDT) represents an effective treatment to cure cancer. The targeting ability of the photosensitizer is of utmost importance. Photosensitizers that discriminate cancer cells can avoid the killing of normal cells and improve PDT efficacy. However, the design and synthesis of photosensitizers conjugated with a recognition unit of cancer cell markers is complex and may not effectively target cancer. Considering that the total RNA content in cancer cells is commonly higher than in normal cells, this study has developed the photosensitizer with RNA-targeting abilities for the discrimination of cancer cells. was specifically located in cancer cells rather than normal cells due to their stronger electrostatic interactions with RNA, thereby further improving the PDT effects on the cancer cells. After intravenous injection into mice bearing a xenograft tumor, accumulated into the tumor location through the enhanced permeability and retention effect, automatically targeted cancer cells under the control of RNA, and inhibited tumor growth under 630 nm laser irradiation without obvious side effects. This intelligent photosensitizer with RNA-targeting ability not only simplifies the design and synthesis of cancer-cell-targeting photosensitizers but also paves the way for the further development of highly efficient PDTs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26195990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512109PMC
October 2021

Dual C(sp)-H Functionalization of Cyclic Ethers via Singlet Oxygen-Mediated Ring Opening and Ring Closing.

Org Lett 2021 Nov 11;23(21):8267-8272. Epub 2021 Oct 11.

Key Laboratory of Medicinal for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.

A metal-free dual C(sp)-H bond functionalization of saturated cyclic ethers via photooxidative singlet oxygen-mediated ring opening and ring closing has been developed, providing a method for generating hydrobenzofurans/pyrans/dioxins. Mechanistic studies have confirmed that ring-opening intermediates were effectively generated by singlet oxygen-mediated C(sp)-H activation and efficiently reacted with aldehydes and activated methylene compounds to form a wide array of products with high diastereoselectivities (up to >95:5 dr). This study is a rare example of α,β-dual C(sp)-H bond functionalization of ethers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c03008DOI Listing
November 2021

Profile of Immunoglobulin G -Glycome in COVID-19 Patients: A Case-Control Study.

Front Immunol 2021 23;12:748566. Epub 2021 Sep 23.

School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, China.

Coronavirus disease 2019 (COVID-19) remains a major health challenge globally. Previous studies have suggested that changes in the glycosylation of IgG are closely associated with the severity of COVID-19. This study aimed to compare the profiles of IgG -glycome between COVID-19 patients and healthy controls. A case-control study was conducted, in which 104 COVID-19 patients and 104 age- and sex-matched healthy individuals were recruited. Serum IgG -glycome composition was analyzed by hydrophilic interaction liquid chromatography with the ultra-high-performance liquid chromatography (HILIC-UPLC) approach. COVID-19 patients have a decreased level of IgG fucosylation, which upregulates antibody-dependent cell cytotoxicity (ADCC) in acute immune responses. In severe cases, a low level of IgG sialylation contributes to the ADCC-regulated enhancement of inflammatory cytokines. The decreases in sialylation and galactosylation play a role in COVID-19 pathogenesis the activation of the lectin-initiated alternative complement pathway. IgG -glycosylation underlines the complex clinical phenotypes of SARS-CoV-2 infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.748566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495247PMC
October 2021

XEDA, a fast and multipurpose energy decomposition analysis program.

J Comput Chem 2021 Dec 9;42(32):2341-2351. Epub 2021 Oct 9.

The State Key Laboratory of Physical Chemistry of Solid Surfaces, Fujian Provincial Key Laboratory of Theoretical and Computational Chemistry, and College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

A fast and multipurpose energy decomposition analysis (EDA) program, called XEDA, is introduced for quantitative analysis of intermolecular interactions. This program contains a series of variational EDA methods, including LMO-EDA, GKS-EDA and their extensions, to analyze non-covalent interactions and strong chemical bonds in various environments. XEDA is highly efficient with a similar computational scaling of single point energy calculations. Its efficiency and universality are validated by a series of test examples including van der Waals interactions, hydrogen bonds, radical-radical interactions and strong covalent bonds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcc.26765DOI Listing
December 2021

n-type absorber by Cd doping achieves high-performance carbon-based CsPbIBr perovskite solar cells.

J Colloid Interface Sci 2021 Sep 24;608(Pt 1):40-47. Epub 2021 Sep 24.

Engineering Research Center of Environment-Friendly Functional Materials, Ministry of Education, PR China; Fujian Key Laboratory of Photoelectric Functional Materials, PR China; College of Materials Science & Engineering, Huaqiao University, Xiamen 361021, PR China. Electronic address:

High efficiency and stability have long been the key issues faced by perovskite solar cells (PSCs). It is found that the CsPbIBr all-inorganic perovskite has a suitable band gap and satisfactory stability, so it has attracted much attention. However, the many defects in the CsPbIBr film are one of the main problems hindering the improvement of power conversion efficiency (PCE) of the CsPbIBr PSCs. The substitution of trace impurities is undoubtedly a simple, cost-effective and efficient strategy. In this work, an appropriate amount of Cd (1.0% mol of Pb) is added into the CsPbIBr precursor solution to fabricate high quality CsPbIBr film with improved crystallinity, reduced trap density, suppressed photo-generated carrier recombination, displayed n-type doping and optimized energy level alignment. The corresponding carbon-based all-inorganic Cd-doped CsPbIBr PSCs achieve a maximum PCE of 10.63% with a high open circuit voltage (V) of 1.324 V, which are much higher than those of the control one with a PCE of 8.48% and an V of 1.235 V. The unencapsulated device can still retain more than 92% of the initial PCE when stored at ambient atmosphere (25 °C, relative humidity about 30%) for 40 days.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.09.117DOI Listing
September 2021

P-glycoprotein mediates the pharmacokinetic interaction of olanzapine with fluoxetine in rats.

Toxicol Appl Pharmacol 2021 Nov 2;431:115735. Epub 2021 Oct 2.

Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East China Normal University, Shanghai, China. Electronic address:

Clinical trials of olanzapine combined with fluoxetine (Olanzapine/Fluoxetine Combination, OFC) in the treatment of refractory depression have shown significant efficacy, but the drug-drug interaction (DDI) between them remains unclear. In this report, the pharmacokinetic interaction between olanzapine and fluoxetine was studied in wild-type (WT) and Mdr1a/b gene knockout (KO) rats. By analyzing the pharmacokinetics and tissue distribution of olanzapine in single dose and combination, the potential DDI mediated by P-gp was explored. The results showed that in WT rats, the combination of fluoxetine increased the peak concentration (C, 44.1 ± 5.1 ng/mL in the combination group vs 9.0 ± 1.5 ng/mL in the monotherapy group) and the exposure (AUC, 235.8 ± 22.7 h × ng/mL in the combination group vs 47.5 ± 8.4 h × ng/mL in monotherapy group) of olanzapine, and decreased the clearance (CL, 8119.0 ± 677.9 mL/h/kg in the combination group vs 49,469.0 ± 10,306.0 mL/h/kg in monotherapy group). At the same time, fluoxetine significantly increased the in vivo exposure of olanzapine in brain, liver, kidney and ileum of WT rats, indicating the occurrence of DDI. The same phenomenon was observed in Caco-2 cells in vitro as well. However, in KO rats, there was no significant difference in pharmacokinetic parameters between the monotherapy group and the combination group. In conclusion, P-gp plays an important role in the pharmacokinetic interaction between olanzapine and fluoxetine in rats. This study may provide a reference for the clinical safety of olanzapine combined with fluoxetine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.taap.2021.115735DOI Listing
November 2021

Easy access to secondary and tertiary alcohols metal-free and light mediated radical carbonyl allylation.

Chem Commun (Camb) 2021 Oct 14;57(82):10783-10786. Epub 2021 Oct 14.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371, Singapore.

Here we report a strategy for carbonyl addition with unactivated alkenes using an organic photocatalyst on both aldehyde and ketone substrates. This protocol grants us a good alternative to the traditional Barbier-Grignard allylation that exhibits poor functional group tolerance. With this method the stoichiometric use of metals can be avoided, high atom economy can be achieved and fewer by-products are generated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc04585gDOI Listing
October 2021

External validation of a novel signature of illness in continuous cardiorespiratory monitoring to detect early respiratory deterioration of ICU patients.

Physiol Meas 2021 09 27;42(9). Epub 2021 Sep 27.

University of Virginia, UVa Center for Advanced Medical Analytics, Charlottesville, VA, United States of America.

Objective: The goal of predictive analytics monitoring is the early detection of patients at high risk of subacute potentially catastrophic illnesses. An excellent example of a targeted illness is respiratory failure leading to urgent unplanned intubation, where early detection might lead to interventions that improve patient outcomes. Previously, we identified signatures of this illness in the continuous cardiorespiratory monitoring data of intensive care unit (ICU) patients and devised algorithms to identify patients at rising risk. Here, we externally validated three logistic regression models to estimate the risk of emergency intubation developed in Medical and Surgical ICUs at the University of Virginia.

Approach: We calculated the model outputs for more than 8000 patients in the University of California-San Francisco ICUs, 240 of whom underwent emergency intubation as determined by individual chart review.

Main Results: We found that the AUC of the models exceeded 0.75 in this external population, and that the risk rose appreciably over the 12 h before the event.

Significance: We conclude that there are generalizable physiological signatures of impending respiratory failure in the continuous cardiorespiratory monitoring data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6579/ac2264DOI Listing
September 2021
-->