Publications by authors named "Yuan Tao"

518 Publications

Physiologically Self-Regulated, Fully Implantable, Battery-Free System for Peripheral Nerve Restoration.

Adv Mater 2021 Oct 4:e2104175. Epub 2021 Oct 4.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, P. R. China.

The long-segment peripheral nerve injury (PNI) represents a global medical challenge, leading to incomplete nerve tissue recovery and unsatisfactory functional reconstruction. However, the current electrical stimulation (ES) apparatuses fail perfect nerve repair due to their inability of the variable synchronous self-regulated function with physiological states. It is urgent to develop an implantable ES platform with physiologically adaptive function to provide instantaneous and nerve-preferred ES. Here, a physiologically self-regulated electrical signal is generated by integrating a novel tribo/piezoelectric hybrid nanogenerator with a nanoporous nerve guide conduit to construct a fully implantable neural electrical stimulation (FI-NES) system. The optimal neural ES parameters completely originate from the body itself and are highly self-responsive to different physiological states. The morphological evaluation, representative protein expression level, and functional reconstruction of the regenerated nerves are conducted to assess the PNI recovery process. Evidence shows that the recovery effect of 15 mm length nerve defects under the guidance of the FI-NES system is significantly close to the autograft. The designed FI-NES system provides an effective method for long-term accelerating the recovery of PNI in vivo and is also appropriate for other tissue injury or neurodegenerative diseases.
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http://dx.doi.org/10.1002/adma.202104175DOI Listing
October 2021

FLAIR and ADC Image-Based Radiomics Features as Predictive Biomarkers of Unfavorable Outcome in Patients With Acute Ischemic Stroke.

Front Neurosci 2021 16;15:730879. Epub 2021 Sep 16.

Department of Medical Imaging, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

At present, it is still challenging to predict the clinical outcome of acute ischemic stroke (AIS). In this retrospective study, we explored whether radiomics features extracted from fluid-attenuated inversion recovery (FLAIR) and apparent diffusion coefficient (ADC) images can predict clinical outcome of patients with AIS. Patients with AIS were divided into a training ( = 110) and an external validation ( = 80) sets. A total of 753 radiomics features were extracted from each FLAIR and ADC image of the 190 patients. Interquartile range (IQR), Wilcoxon rank sum test, and least absolute shrinkage and selection operator (LASSO) were used to reduce the feature dimension. The six strongest radiomics features were related to an unfavorable outcome of AIS. A logistic regression analysis was employed for selection of potential predominating clinical and conventional magnetic resonance imaging (MRI) factors. Subsequently, we developed several models based on clinical and conventional MRI factors and radiomics features to predict the outcome of AIS patients. For predicting unfavorable outcome [modified Rankin scale (mRS) > 2] in the training set, the area under the receiver operating characteristic curve (AUC) of ADC radiomics model was 0.772, FLAIR radiomics model 0.731, ADC and FLAIR radiomics model 0.815, clinical model 0.791, and clinical and conventional MRI model 0.782. In the external validation set, the AUCs for the prediction with ADC radiomics model was 0.792, FLAIR radiomics model 0.707, ADC and FLAIR radiomics model 0.825, clinical model 0.763, and clinical and conventional MRI model 0.751. When adding radiomics features to the combined model, the AUCs for predicting unfavorable outcome in the training and external validation sets were 0.926 and 0.864, respectively. Our results indicate that the radiomics features extracted from FLAIR and ADC can be instrumental biomarkers to predict unfavorable clinical outcome of AIS and would additionally improve predictive performance when adding to combined model.
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http://dx.doi.org/10.3389/fnins.2021.730879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483716PMC
September 2021

Diversity of endophytic bacteria in wild rice (Oryza meridionalis) and potential for promoting plant growth and degrading phthalates.

Sci Total Environ 2021 Sep 14;806(Pt 1):150310. Epub 2021 Sep 14.

Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Phthalates (PAEs) accumulated in agricultural soils and rice have increased human exposure risks. Microbial degradation could efficiently reduce the residue of organic pollutants in soil and crop plants. Here, we hypothesized that endophytic bacteria from wild rice have the potential for degradation of PAEs and plant growth promoting. The endophytic bacterial community and functional diversity in wild rice (Oryza meridionalis) were analyzed for the first time, and the potential for PAE degradation and plant growth promoting by endophytes were investigated. The results of Illumina high-throughput sequencing revealed that abundant endophytes inhabited in wild rice with Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria being the dominant phyla. Endophytic bacterial diversity and complexity were confirmed by isolation and clustering of isolates. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that endophytes exerted diverse functions such as plant growth promoting, xenobiotics biodegradation, pollution remediation and bacterial chemotaxis. Pure culture experiment showed that 30 isolated endophytic strains exhibited in vitro plant growth promoting activities, and rice plants inoculated with these strains confirmed their growth promoting abilities. Some endophytic strains were capable of efficiently degrading PAEs, with the highest removal percentage of di-n-butyl phthalate (DBP) up to 96.1% by Bacillus amyloliquefaciens strain L381 within 5 days. Synthetic community F and strain L381 rapidly removed DBP from soil (removing 91.0%-99.2% within 10 d and from rice plant slurry (removing 93.4%-99.2% within 5 d). These results confirmed the hypothesis and demonstrated the diversity of endophytic bacteria in wild rice with diverse functions, especially for plant growth promoting and removing PAEs. These multifunctional endophytic bacteria provided good alternatives to reduce PAE accumulation in crops and increase yield.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150310DOI Listing
September 2021

[Four new lanostane triterpenoids from latex of Euphorbia resinifera].

Zhongguo Zhong Yao Za Zhi 2021 Sep;46(18):4744-4748

College of Life Science, Jiangxi Normal University Nanchang 330022, China.

Four new lanostane triterpenoids, 3β-hydroxy-12α-methoxylanosta-7,9(11),24-triene(1), 3β-hydroxy-12α-methoxy-24-methylene-lanost-7,9(11)-dien(2), 3,7-dioxo-lanosta-8,24-diene(3), and 3,7-dioxo-24-methylene-lanost-8-en(4), were isolated from the latex of Euphorbia resinifera with a variety of chromatography methods. Their structures were elucidated based on spectroscopic data and/or comparison with the data reported in previous research. Compounds 1, 2, and 4 showed moderate inhibition of LPS-induced NO production by RAW264.7, with IC_(50) of 30.4, 37.5, and 28.3 μmol·L~(-1), respectively.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210623.201DOI Listing
September 2021

Polydopamine-based nanoplatform for photothermal ablation with long-term immune activation against melanoma and its recurrence.

Acta Biomater 2021 Sep 16. Epub 2021 Sep 16.

Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, People's Republic of China. Electronic address:

The high risk of tumor recurrence presents a big challenge in melanoma therapy. Photothermal therapy (PTT) has merged as a powerful weapon against tumor in recent years, which produces tumor-associated antigens (TAA) and recruits dendritic cells (DCs) to tumor sites through immunogenic cell death (ICD) for immune activation. However, due to the lack of activation signals of DCs, the immune effect induced by PTT is not sufficient to inhibit the recurrence and proliferation of tumor. To efficiently cooperate PTT and immunotherapy to circumvent tumor recurrence, here we constructed a polydopamine (PDA) based core-shell nanoplatform loading CpG ODNs to elicit robust photothermal ablation and antitumor immune responses. Cationized polydopamine coated with hyaluronic acid (HA) shell was proven an efficient photothermal agent that increased the surface temperature of tumor by 16 °C and induced ICD. CpG ODNs effectively induced maturation of DCs by elevating the expression of co-stimulating markers. PTT combined with CpG ODNs achieved a remarkable synergistic treatment effect in the maturation of DCs and activation of T cells in melanoma-bearing mice model compared with PTT or CpG ODNs alone. Furthermore, in a tumor recurrence model, photothermal-immune combination therapy increased the infiltration of CTLs in distant tumor compared with PTT or CpG ODNs alone. The combination therapy overcame insufficient immunity at distant tumor caused by PTT alone and relieved immunosuppression microenvironment of the tumor. Hence, the PDA based core-shell nanoplatform presents a potent photo-immunotherapy against proliferation and recurrence of melanoma. STATEMENT OF SIGNIFICANCE: In order to solve the insufficient immunity induced by photothermal therapy (PTT), CpG ODNs were utilized to enhance the weak immune response mediated by PTT through inducing DCs maturation. Hence, we designed a polydopamine (PDA) based core-shell nanoplatform loading CpG ODNs followed by hyaluronic acid named PPP/CpG/HA to elicit robust photothermal ablation and antitumor immune responses. CpG ODNs were delivered to the tumor site through the targeting effect of the HA shell. The core-shell nanoplatform achieved a remarkable synergistic treatment effect in the maturation of DCs and activation of T cells, thereby overcoming insufficient immunity at distant tumor caused by PTT alone. The core-shell nanoplatform presents a potent photo-immunotherapy against proliferation and recurrence of melanoma.
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http://dx.doi.org/10.1016/j.actbio.2021.09.014DOI Listing
September 2021

Co-delivery of autophagy inhibitor and gemcitabine using a pH-activatable core-shell nanobomb inhibits pancreatic cancer progression and metastasis.

Theranostics 2021 4;11(18):8692-8705. Epub 2021 Aug 4.

Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, People's Republic of China.

Metastasis is one of the main reasons for the high mortality associated with pancreatic ductal adenocarcinoma (PDAC), and autophagy regulates the metastatic migration of tumor cells, their invasion of tissues, and their formation of focal adhesions. Inhibiting autophagy may suppress tumor growth and metastasis, but the abundant extracellular matrix hinders the deep penetration of therapeutic agents. To enhance the penetration of drugs that can inhibit metastasis of pancreatic cancer, a pH-responsive drug delivery system was formulated. Gemcitabine (GEM), a first-line chemotherapeutic drug against PDAC, was loaded in 6PA-modified DGL (PDGL) nanoparticles to afford PDGL-GEM. Then PDGL-GEM was co-precipitated with the autophagy inhibitor chloroquine phosphate (CQ) and calcium phosphate to formulate [email protected]/CQ. The size and morphology of the resulting "nanobomb" [email protected]/CQ were characterized, and their uptake into cells, cytotoxicity and ability to inhibit autophagy were analyzed at pH 6.5 and 7.4. The anti-tumor and anti-metastasis effects of the nanobomb were explored on mice carrying Pan 02 pancreatic tumor xenografts or orthotopic tumors. The pH-induced dissolution of calcium phosphate facilitated the release of CQ from the nanobomb and deep penetration of PDGL-GEM. The internalization of PDGL-GEM and subsequent intracellular release of GEM inhibited tumor growth, while CQ downregulated autophagy in tumor cells and fibroblasts. In fact, inhibition of xenograft and orthotopic tumor growth was greater with the complete [email protected]/CQ than with subassemblies lacking GEM or CQ. More importantly, mechanistic studies and suggested that the nanobomb inhibits metastasis by downregulating MMP-2 and paxillin, as well as reducing fibrosis. The pH-sensitive [email protected]/CQ shows potential for inhibiting proliferation and metastasis of pancreatic cancer through an autophagy-dependent pathway.
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http://dx.doi.org/10.7150/thno.60437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419034PMC
August 2021

Case Report: Bilateral Ciliary Body Detachment in a Patient Taking Oral Diet Pills.

Optom Vis Sci 2021 08;98(8):876-880

Department of Ophthalmology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China.

Significance: The use of antiobesity drugs is becoming more widespread, and the resulting adverse effects are also increasing. Visual impairments caused by weight-loss pills need a timely and accurate diagnosis and treatment. Prompt diagnosis and treatment can achieve a satisfactory prognosis.

Purpose: This report describes a case of a significant decline in bilateral visual acuity caused by taking diet pills and explores the possible pathogenesis.

Case Report: A 32-year-old Chinese woman showed shallow anterior chambers, and intraocular pressure (IOP) was 38 mmHg bilaterally after taking Korean prescription diet pills for 6 days. The best-corrected visual acuity of both eyes was 0.03. The ultrasound biomicroscopy showed complete ciliary body detachment accompanied with angle closure. The central anterior chamber depths were 1.70 mm in the right eye and 1.61 mm in the left eye. The patient was diagnosed with ciliary body detachment with secondary elevated IOP. The patient was treated with pilocarpine, carteolol hydrochloride, brinzolamide, mannitol, and dexamethasone sodium phosphate. The patient had rewarding prognosis after treatment with discontinuation of diet pills, control of IOP, and glucocorticoids.

Conclusions: Extensive publicity and education are needed to ensure that consumers do not abuse diet pills; meanwhile, a timely diagnosis and withdrawal are crucial for a desirable prognosis. Clinicians need to consider the possibility of drug-secondary ocular diseases.
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http://dx.doi.org/10.1097/OPX.0000000000001743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407448PMC
August 2021

HOMA-IR as a risk factor of gestational diabetes mellitus and a novel simple surrogate index in early pregnancy.

Int J Gynaecol Obstet 2021 Aug 27. Epub 2021 Aug 27.

National Center for Women and Children's Health, China CDC, Beijing, China.

Objective: To assess the association between insulin resistance and gestational diabetes mellitus (GDM) in early pregnancy and find a simple surrogate index of the homeostasis model assessment of insulin resistance (HOMA-IR).

Methods: A total of 700 pregnant women were included in this prospective, double-center, observational cohort study. The glucose and lipid metabolic characterization was performed at 6-12 weeks of pregnancy. All participants underwent a 75-g oral glucose tolerance test at 24-28 weeks of pregnancy. Linear regression analysis was applied to find a novel surrogate index of HOMA-IR. Binary logistic analysis was applied to estimate possible associations of different indices with GDM and insulin resistance.

Results: GDM was diagnosed in 145 of 700 women with singleton pregnancies (20.7%). HOMA-IR was higher in the GDM group than in the normal glucose tolerance (NGT) group and was an individual risk factor for GDM (adjusted risk ratio RR 1.371, 95% confidence interval [CI] 1.129-1.665, P < 0.001). TyHGB index as the surrogate index of HOMA-IR was represented as TG/HDL-C + 0.7*FBG (mmol/L) +0.1*preBMI (kg/m )(where TG/HDL-C is triglyceride/high-density lipoprotein cholesterol; FBG is fasting blood glucose, and preBMI is the pre-pregnancy body mass index [calculated as weight in kilograms divided by the square of height in meters]). The cut-off point of the TyHGB index was 6.0 (area under the curve 0.827, 95% CI 0.794-0.861, P < 0.001) for mild insulin resistance.

Conclusion: Increased HOMA-IR in early pregnancy was a risk factor of GDM. TyHGB index could be a surrogate index of HOMA-IR and had a predictive value for GDM.
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http://dx.doi.org/10.1002/ijgo.13905DOI Listing
August 2021

Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.

Carbohydr Polym 2021 Nov 31;272:118514. Epub 2021 Jul 31.

Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China. Electronic address:

Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices. In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed. This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface. As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm) and well electrochemical properties. Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition. Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
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http://dx.doi.org/10.1016/j.carbpol.2021.118514DOI Listing
November 2021

Catalytic Role of Adsorption of Electrolyte/Molecules as Functional Ligands on Two-Dimensional TM-N Monolayer Catalysts for the Electrocatalytic Nitrogen Reduction Reaction.

ACS Appl Mater Interfaces 2021 Sep 20;13(34):40590-40601. Epub 2021 Aug 20.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Two-dimensional single-atom catalysts (2D SACs) have been widely studied on the nitrogen reduction reaction (NRR). The characteristics of 2D catalysts imply that both sides of the monolayer can be catalytic sites and adsorb electrolyte ions or molecules from solutions. Overstrong adsorption of electrolyte ions or molecules on both sides of the catalyst site will poison the catalyst, while the adsorbate on one side of the catalytic site will modify the activity and selectivity of the other side for NRR. Discovering the influence of adsorption of electrolyte ions or molecules as a functional ligand on catalyst performance on the NRR is crucial to improve NRR efficiency. Here, we report this work using the density functional theory (DFT) method to investigate adsorption of electrolyte ions or molecules as a functional ligand. Among all of the studied 18 functional ligands and 3 transition metals (TMs), the results showed that Ru&F, Ru&COOH, and Mo&HO combinations were screened as electrocatalysis systems with high activity and selectivity. Particularly, the Mo&HO combination possesses the highest activity with a low Δ of 0.44 eV through the distal pathway. The superior catalytic performance of the Mo&HO combination is mainly attributed to the electron donation from the metal d orbital. Furthermore, the functional ligands can occupy the active sites and block the competing vigorous hydrogen evolution reaction. Our findings offer an effective and practical strategy to design the combination of the catalyst and electrolyte to improve electrocatalytic NRR efficiency.
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http://dx.doi.org/10.1021/acsami.1c10367DOI Listing
September 2021

MicroRNA‑8063 targets heterogeneous nuclear ribonucleoprotein AB to inhibit the self‑renewal of colorectal cancer stem cells via the Wnt/β‑catenin pathway.

Oncol Rep 2021 10 13;46(4). Epub 2021 Aug 13.

Department of Gastrointestinal Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China.

The presence of cancer stem cells (CSCs) is a major cause of therapeutic failure in a variety of cancer types, including colorectal cancer (CRC). However, the underlying mechanisms that regulate the self‑renewal of colorectal cancer stem cells (CRCSCs) remain unclear. Our previous study utilized CRCSCs and their parent cells; through gene microarray screening and bioinformatics analysis, we hypothesized that microRNA (miR)‑8063 may bind to, and regulate the expression of, heterogeneous nuclear ribonucleoprotein AB (hnRNPAB) to facilitate the regulation of CRCSC self‑renewal. The aim of the present study was to confirm this conjecture through relevant experiments. The results indicated that compared with that in parent cells, miR‑8063 expression was significantly downregulated in CRCSCs, while hnRNPAB expression was increased. Furthermore, hnRNPAB was identified as a direct target of miR‑8063 using a dual‑Luciferase assay. Overexpression of hnRNPAB promoted the acquisition of CSC characteristics in CRC cells (increased colony formation ability, enhanced tumorigenicity, and upregulated expression of CSC markers), as well as the upregulation of key proteins (Wnt3a, Wnt5a and β‑catenin) in the Wnt/β‑catenin signaling pathway. Similarly, after silencing miR‑8063 in CRC cells, the characteristics of CSC were altered, and the expression of hnRNPAB protein was promoted. However, post overexpression of miR‑8063 in CRCSCs, the self‑renewal ability of CSCs was weakened with the downregulation of hnRNPAB protein, Wnt3a, Wnt5a and β‑catenin. These results suggest that as a tumor suppressor, miR‑8063 is involved in regulating the self‑renewal of CRCSCs, where loss of miR‑8063 expression weakens its inhibition on hnRNPAB, which leads to the activation of Wnt/β‑catenin signaling to promote the self‑renewal of CRCSCs.
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http://dx.doi.org/10.3892/or.2021.8170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377466PMC
October 2021

Thromboelastography parameters in diagnosing periprosthetic joint infection and predicting reimplantation timing.

BMC Musculoskelet Disord 2021 Aug 13;22(1):689. Epub 2021 Aug 13.

Department of Joint Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.

Background: Coagulation-related biomarkers are drawing new attention in the diagnosis of periprosthetic joint infection (PJI). The thromboelastography (TEG) assay provides a comprehensive assessment of blood coagulation; therefore, it could be a promising test for PJI. This study aims to assess the value of TEG in diagnosing PJI and to determine the clinical significance of TEG in analysing reimplantation timing for second-stage revision.

Methods: From October 2017 to September 2020, 62 patients who underwent revision arthroplasty were prospectively included. PJI was defined by the 2011 Musculoskeletal Infection Society criteria, in which 23 patients were diagnosed with PJI (Group A), and the remaining 39 patients were included as having aseptic loosening (Group B). In group A, 17 patients completed a two-stage revision in our centre. C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), D-dimer, and TEG parameters (clotting time, α-angle, MA [maximum amplitude], amplitude at 30 min, and thrombodynamic potential index) were measured preoperatively in all included patients. In addition, receiver operating characteristic curves were used to evaluate the diagnostic value of these biomarkers.

Results: ESR (area under curve [AUC], 0.953; sensitivity, 81.82; specificity, 94.87) performed best for PJI diagnosis, followed by MA (AUC, 0.895; sensitivity, 82.61; specificity, 97.44) and CRP (AUC, 0.893; sensitivity, 82.61; specificity, 94.74). When these biomarkers were combined in pairs, the diagnostic value improved compared with any individual biomarker. The overall success rate of the two-stage revision was 100%. Furthermore, ESR and MA were valuable in determining the time of reimplantation, and their values all decreased below the cut-off values before reimplantation.

Conclusion: TEG could be a promising test in assisting PJI diagnosis, and a useful tool in judging the proper timing of reimplantation.
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http://dx.doi.org/10.1186/s12891-021-04578-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364122PMC
August 2021

Aromatic Cadinane Sesquiterpenoids from the Fruiting Bodies of Block SARS-CoV-2 Spike-ACE2 Interaction.

J Nat Prod 2021 08 5;84(8):2385-2389. Epub 2021 Aug 5.

National R&D Center for Freshwater Fish Processing, and Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.

The ongoing COVID-19 global pandemic caused by SARS-CoV-2 inspires the development of effective inhibitors to block the SARS-CoV-2 spike-ACE2 interaction. A chemical investigation on the fruiting bodies of led to the isolation of five aromatic cadinane sesquiterpenoids including four new ones, named piniterpenoids A-D (-), as well as three known lignans. Their structures were determined by extensive spectroscopic analysis including HRMS and 1D and 2D NMR. All of the aromatic cadinane sesquiterpenoids inhibited the SARS-CoV-2 spike-ACE2 interaction, with IC values ranging from 64.5 to 99.1 μM. A molecular docking study showed the disruption of the interaction of compound via hydrogen interactions with Arg403, Asp405, and Arg408 of SARS-CoV-2 RBD and Arg393 and His34 residues of ACE2. These results suggested that aromatic cadinane sesquiterpenoids might be useful in developing agents for COVID-19.
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http://dx.doi.org/10.1021/acs.jnatprod.1c00426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353988PMC
August 2021

Selective Functional Network Changes Following tDCS-Augmented Language Treatment in Primary Progressive Aphasia.

Front Aging Neurosci 2021 12;13:681043. Epub 2021 Jul 12.

Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD, United States.

Objective: Transcranial direct current stimulation (tDCS) has shown promising results when used as an adjunct to behavioral training in neurodegenerative diseases. However, the underlying neural mechanisms are not understood and neuroimaging evidence from pre/post treatment has been sparse. In this study, we examined tDCS-induced neural changes in a language intervention study for primary progressive aphasia (PPA), a neurodegenerative syndrome with language impairment as the primary clinical presentation. Anodal tDCS was applied to the left inferior frontal gyrus (LIFG). To evaluate the hypothesis that tDCS promotes system segregation, analysis focused on understanding tDCS-induced changes in the brain-wide functional network connectivity of the targeted LIFG.

Methods: Resting-state fMRI data were obtained from 32 participants with PPA before and after receiving a written naming therapy, accompanied either by tDCS or sham stimulation. We focused on evaluating changes in the global connectivity of the stimulated LIFG-triangularis (LIFG-tri) region given its important role in lexical processing. Global connectivity was indexed by the graph-theoretic measure (PC) which quantifies a region's level of system segregation. The values before and after treatment were compared for each condition (tDCS or Sham) as well as with age-matched healthy controls ( = 19).

Results: Higher global connectivity of the LIFG-tri before treatment was associated with greater dementia severity. After treatment, the tDCS group showed a significant decrease in global connectivity whereas the Sham group's did not change, suggesting specific neural effects induced by tDCS. Further examination revealed that the decrease was driven by reduced connectivity between the LIFG-tri and regions outside the perisylvian language area, consistent with the hypothesis that tDCS enhances the segregation of the language system and improves processing efficiency. Additionally, we found that these effects were specific to the LIFG-tri and not observed in other control regions.

Conclusion: TDCS-augmented language therapy in PPA increased the functional segregation of the language system, a normalization of the hyper-connectivity observed before treatment. These findings add to our understanding of the nature of tDCS-induced neural changes in disease treatment and have applications for validating treatment efficacy and designing future tDCS and other non-invasive brain stimulation (NIBS) treatments.
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http://dx.doi.org/10.3389/fnagi.2021.681043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311858PMC
July 2021

Power Generation from Moisture Fluctuations Using Polyvinyl Alcohol-Wrapped Dopamine/Polyvinylidene Difluoride Nanofibers.

Small 2021 Sep 27;17(36):e2102550. Epub 2021 Jul 27.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, P. R. China.

Despite the boom in the water-triggered electric power generation technologies, few attempts have been made with a broader horizonyielding the electricity from sweat, which is of great value for low-power-consumption wearable electronics. Here, an electromechanical coupling and humidity-actuated two-in-one humidity actuator-driven piezoelectric generator (HAPG) are reported, that can yield continuous electric power from fluctuations in the ambient humidity. It is composed of polyvinyl alcohol (PVA)-wrapped highly aligned dopamine (DA)/polyvinylidene fluoride (PVDF) shell/core nanofibers ([email protected]/PVDF NFs). As-received [email protected]/PVDF NFs can exchange water with the ambient humidity to perform expansion and contraction and convert them into electric power. An all-fiber-based portable HAPG is fabricated and tested on human palm skin. The devices show high sensitivity and accuracy for converting the mental sweating-derived continuous moisture fluctuations into electric power. This electric power can be stored in capacitors, which is expected to power micro- and nano-electronic devices or be used in electrotherapy such as electrical stimulation to promote wound healing. Beyond this, the obtained voltage profiles exhibit unique features that can reflect the typical sweat damping oscillation curve features.
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http://dx.doi.org/10.1002/smll.202102550DOI Listing
September 2021

Enzalutamide-Induced Upregulation of PCAT6 Promotes Prostate Cancer Neuroendocrine Differentiation by Regulating miR-326/HNRNPA2B1 Axis.

Front Oncol 2021 30;11:650054. Epub 2021 Jun 30.

Department of Urology, Tongji Hospital, Tongji University of Medicine, Shanghai, China.

Our previous studies have demonstrated that Enzalutamide-induced upregulation of long non-coding RNA p21 (lncRNA-p21) facilitates prostate cancer (PCa) neuroendocrine differentiation (NED). Given the important role of lncRNAs in PCa pathogenesis, and given that lots of lncRNAs are dys-regulated in neuroendocrine PCa (NEPC) patients, we next explored the biological function and underlying mechanism of lncRNA-PCAT6 (PCAT6) in mediating Enzalutamide-induced NED. The level of PCAT6 in Enzalutamide-treated PCa cells and NEPC samples were assessed using quantitative RT-PCR (qPCR). The effect of PCAT6 on PCa cell proliferation, invasion, and NED was evaluated through CCK-8, transwell, qPCR, western blot analysis, Xenograft mouse model, and lung metastasis model. We found that PCAT6 was highly expressed in NE-like cells (PC3, DU145, and NCI-H660) compared with androgen-sensitive LNCaP cells. PCAT6 was also highly expressed in NEPC tissues. Enzalutamide treatment resulted in a significant increase of PCAT6 level in a dose- and time-dependent fashion. Functionally, PCAT6 overexpression promoted NED of C4-2 cells, as evidenced by an increased expression of NE markers (NSE, ChgA, and SYP), whereas PCAT6 knockdown in NCI-H661 cells repressed NED. Furthermore, PCAT6 overexpression promoted PCa cell proliferation and invasion and . Mechanistically, PCAT6 functioned as competing endogenous (ce) RNA absorbing miR-326, thus resulting in a de-suppression of target gene. The current results demonstrate that PCAT6 acted as a tumor activator in PCa progression by sponging miR-326 and increasing Hnrnpa2b1 expression and that the PCAT6/miR-326/Hnrnpa2b1 signaling might be a new therapeutic target for PCa.
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http://dx.doi.org/10.3389/fonc.2021.650054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278330PMC
June 2021

Surface-confined guanidinium ionic liquid as a new type of stationary phase for hydrophilic interaction liquid chromatography.

J Sep Sci 2021 Sep 30;44(18):3357-3365. Epub 2021 Jul 30.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, P. R. China.

Guanidinium-based ionic liquids possess lower toxicity and greater designability than commonly used species and have presented good performances in liquid-phase extraction and stationary phase for capillary gas chromatography. In the present work, a novel type of surface-confined guanidinium ionic liquid stationary phase was developed by bonding a hexaalkylguanidinium ionic liquid N,N,N',N'-tetramethyl-N",N"-diallylguanidinium bromide onto the surface of 3-mercaptopropyl modified silica. The obtained surface-confined guanidinium ionic liquid silica materials were characterized by elemental analysis, infrared spectroscopy and thermogravimetric analysis, and then packed as a high-performance liquid chromatography column for the evaluation of chromatographic retention behavior. Typical polar compounds were used to evaluate the separation performances, and the changes of retention with water content in mobile phase further suggested the hydrophilic interaction liquid chromatography retention mechanism. Moreover, the effect of different chromatographic factors (salt concentration, mobile phase pH, and column temperature) on retention was investigated with a series of compounds as test solutes to gain insights into the retention mechanism. The results indicated that the surface-confined guanidinium ionic liquid stationary phase exhibited a hydrophilic interaction liquid chromatography/anion-exchange mixed-mode retention behavior and possessed promising potential in separating a wide range of compounds as an alternative stationary phase for high-performance liquid chromatography.
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http://dx.doi.org/10.1002/jssc.202100385DOI Listing
September 2021

Three-dimensional magnetic stannic disulfide composites for the solid-phase extraction of sulfonamide antibiotics.

J Chromatogr A 2021 Aug 29;1652:462372. Epub 2021 Jun 29.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116023, China; School of Chemical Engineering, Dalian University of Technology, Panjin 124221, China. Electronic address:

In the present work, three-dimensional (3D) and flower-like SnS materials were coated on the surface of [email protected] through an in-situ growth method. The 3D architecture could avoid the accumulation and reaggregation with better stability and was beneficial for the exposure of more active sites. The prepared magnetic SnS composites were used for the enrichment of sulfonamide antibiotics (SAs), and various experimental parameters affecting the extraction efficiency were investigated. The results showed the equilibrium of extraction and desorption towards target SAs could be reached within 3 min by using the [email protected] composites. Under optimized conditions, the proposed approach possessed good linearity in the range of 0.1-200 ng·mL with correlation coefficients r above 0.9964 and low limits of detection (LODs) from 0.025 to 0.250 ng·mL for the five target SAs. Moreover, good repeatability was obtained with the intra-day and inter-day precision in terms of relative standard deviations (RSDs) within 1.1%-10.8% and 7.4%-13.1%, and the recoveries under three spiked concentrations were between 81.8% and 119.7% with adequate accuracy. Different samples including tap water, milk and honey were collected for magnetic solid-phase extraction and determination of target SAs by using the obtained [email protected] composites to demonstrate the utility. All the results indicated that the proposed method had great potential for effective preconcentration and determination of SAs in complex samples.
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http://dx.doi.org/10.1016/j.chroma.2021.462372DOI Listing
August 2021

Constructing Well-Defined and Robust Th-MOF-Supported Single-Site Copper for Production and Storage of Ammonia from Electroreduction of Nitrate.

ACS Cent Sci 2021 Jun 2;7(6):1066-1072. Epub 2021 Jun 2.

State Key Laboratory of Nuclear Resources and Environment, School of Biology, Chemistry and Material Science, East China University of Technology, Nanchang, Jiangxi 330013, China.

A combined technique of production and storage of ammonia (NH) from electroreduction of nitrate (NO ) through one material is highly desirable but remains a huge challenge. Herein, we proposed a proof-of-concept strategy for combined NH production and storage from electroreduction of NO through elaborately designing a single-site Cu-bipyridine-based thorium metal-organic framework ([email protected]). Noticeably, the single Cu site, anchored by a solid-liquid postsynthetic metalation within Th-BPYDC, shows a novel square coordination structure, as determined by the single-crystal X-ray diffraction. This strongly implies its enormous potential as an open metal site and consequently enables excellent performance in electroreduction of NO for NH production, giving 92.5% Faradaic efficiency and 225.3 μmol h cm yield. Impressively, we can further use [email protected] material to effectively capture the previously produced NH from electroreduction of NO , affording an uptake up to 20.55 mmol g at 298 K at 1 bar. The results in this work will outline a new direction toward the combined technique for advanced electrocatalysis such as gas production storage/or separation.
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http://dx.doi.org/10.1021/acscentsci.1c00370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228586PMC
June 2021

Novel Smart Insulating Materials Achieving Targeting Self-Healing of Electrical Trees: High Performance, Low Cost, and Eco-Friendliness.

ACS Appl Mater Interfaces 2021 Jul 7;13(28):33485-33495. Epub 2021 Jul 7.

Analytical and Testing Center, Chongqing University, Chongqing 400030, People's Republic of China.

It remains challenging to promptly inhibit and autonomically heal electrical trees inside insulating dielectrics, which are caused by sustained strong electrical fields and substantially shorten electronic device lifetimes and even cause premature failure of electrical equipment. Therefore, we demonstrate a magnetically targeted ultraviolet (UV)-induced polymerization functional microcapsule (MTUF-MC) to endow insulating materials with physical and electrical dual-damage self-healing capabilities. Specifically, [email protected] and TiO nanoparticles, which serve as magnetic targets and UV shields (thereby preventing the healing agent from prematurely triggering), constitute a functional microcapsule shell, ensuring a low dopant concentration and excellent self-healing ability of the epoxy composites without affecting the intrinsic performance of the matrix. By exploiting electroluminescence originating from electrical trees, UV-induced polymerization of healing agent is handily triggered without any applying external stimuli to intelligently, contactlessly, and autonomously self-healing electrical trees inside insulating dielectrics.
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http://dx.doi.org/10.1021/acsami.1c07469DOI Listing
July 2021

[Dynamics of desertification in the lower reaches of Shiyang River Basin, Northwest China during 1995-2018].

Ying Yong Sheng Tai Xue Bao 2021 Jun;32(6):2098-2106

College of Social Development and Public Administration, Northwest Normal University, Lanzhou 730070, China.

We collected remote sensing images of lower reaches of the Shiyang River Basin in 1995, 2000, 2005, 2010, 2015, and 2018. We analyzed the dynamics of desertification in Minqin County by constructing desertification difference index, using RS and GIS theories and methods. We established the Albedo-NDVI feature space based on the normalized vegetation index (NDVI) and surface albedo (Albedo). The results showed that the desertification area in the lower reaches of Shi-yang River Basin accounted for more than 90% of the total area of the Minqin. Oasis scattered in large areas of desertified land. Moderate desertification accounted for more than 70% of the total area of the county. The area of desertification in Minqin showed an decreasing trend from 1995 to 2018, with an average annual reduction area of 22.06 km and an average annual reduction rate of 0.1%. During the study period, various types of desertification area contracted and expanded simultaneously, and the relative changes of severe, moderate, mild, and slight desertification areas were -1.5%, 0.2%, -0.9%, and 3.8% respectively. Those results indicated that the severity of desertification was reduced. The desertification area was generally at a stable state. There was no large-scale desertification process and no obvious trends of desertification. Desertification control had achieved phased results. In the study area, the area where the desertification decreased in a fluctuation way accounted for 15.2%, while the area with increased desertification only accounted for 3.9%. The areas with irregular fluctuations of desertification were mainly distributed in the transitional area of desert oasis and the edge area of oasis, with an area of 17.7% of the total land area. The desertification change was more active in the ecotone of oasis and desert area, which was the key control and repair region in the future.
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http://dx.doi.org/10.13287/j.1001-9332.202106.012DOI Listing
June 2021

Directionally-Dependent Mechanical Properties of Ti6Al4V Manufactured by Electron Beam Melting (EBM) and Selective Laser Melting (SLM).

Materials (Basel) 2021 Jun 28;14(13). Epub 2021 Jun 28.

Loewy Institute, Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA18015, USA.

An investigation of mechanical properties of Ti6Al4V produced by additive manufacturing (AM) in the as-printed condition have been conducted and compared with wrought alloys. The AM samples were built by Selective Laser Melting (SLM) and Electron Beam Melting (EBM) in 0°, 45° and 90°-relative to horizontal direction. Similarly, the wrought samples were also cut and tested in the same directions relative to the plate rolling direction. The microstructures of the samples were significantly different on all samples. α' martensite was observed on the SLM, acicular α on EBM and combination of both on the wrought alloy. EBM samples had higher surface roughness (Ra) compared with both SLM and wrought alloy. SLM samples were comparatively harder than wrought alloy and EBM. Tensile strength of the wrought alloy was higher in all directions except for 45°, where SLM samples showed higher strength than both EBM and wrought alloy on that direction. The ductility of the wrought alloy was consistently higher than both SLM and EBM indicated by clear necking feature on the wrought alloy samples. Dimples were observed on all fracture surfaces.
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http://dx.doi.org/10.3390/ma14133603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269598PMC
June 2021

Euphatexols C - G, five new triterpenoids from the latex of .

J Asian Nat Prod Res 2021 Jun 18:1-10. Epub 2021 Jun 18.

The Laboratory of Effective Substances of Jiangxi Genuine Medicinal Materials, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.

Five new triterpenoids named as euphatexols C - G (-) were isolated from the latex of . Their chemical structures were determined by extensive spectroscopic analysis, including IR, HRMS, 1 D and 2 D NMR data. Compounds showed moderate anti-inflammatory activity with IC values of 22.30, 48.04, 21.89, 38.15 and 41.15 μM, respectively.
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http://dx.doi.org/10.1080/10286020.2021.1935894DOI Listing
June 2021

Do foreign direct investment and environmental regulation improve green technology innovation? An empirical analysis based on panel data from the Chinese manufacturing industry.

Environ Sci Pollut Res Int 2021 Oct 16;28(39):55302-55314. Epub 2021 Jun 16.

Discipline Construction and Graduate Management Division, Xuzhou University of Technology, Xuzhou, 221018, China.

The environmental regulation and foreign direct investment (FDI) inflow have an important impact on the progress of green technology. This study analyzes the impacts of environmental regulation and FDI on green technology innovation (GTI) based on the panel data of 13 Chinese manufacturing sectors. The results of static panel regression show that the environmental regulation has a positive impact on GTI, while the FDI has a negative impact. The results of the panel threshold model reveal that the effect of environmental regulation on GTI presents a nonlinear shape. The negative effect of FDI on GTI is strengthened when the environmental regulation exceeds its threshold. Increasing FDI inflow can inhibit the effect of environmental regulation. Meanwhile, a strict environmental regulation can enhance the inhibiting effect of FDI on GTI. The FDI inflow into high-tech manufacturing sectors has a less negative impact on GTI than the FDI inflow into low-tech sectors in the case of the enhancement of environmental regulation. This study provides some implications for the formulation of environmental regulation and the FDI inflow into China to improve the GTI.
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http://dx.doi.org/10.1007/s11356-021-14648-1DOI Listing
October 2021

Systematic review and meta-analysis of vitamin D deficiency in different pregnancy on preterm birth: Deficiency in middle pregnancy might be at risk.

Medicine (Baltimore) 2021 Jun;100(24):e26303

Gansu Provincial Maternity and Child-Care Hospital.

Background: Current studies suggest that vitamin D deficiency during pregnancy can produce a certain effect for preterm birth (PTB), but there is no research showing whether vitamin D deficiency has a consistent effect in different pregnancies; thus, we conducted a systematic review and meta-analysis of 24 observational studies, grouping them according to the gestational age at the time of serum sampling, to investigate whether vitamin D deficiency in different periods of gestation has different effects on PTB and to provide an evidence-based basis for pregnant women to measure and supplement vitamin D.

Methods: The databases PubMed-Medline, EMBASE, the Cochrane Library, Web of Science, EBSCO, CBM, and CNKI were searched until February 2020. Two researchers independently assessed the eligibility and quality of studies, and STATA 12.0 software was used for meta-analysis.

Result: Seven cohort studies, 13 case-control studies, and 4 cross-sectional studies were included from 2500 articles by inclusion and exclusion criteria. After adjusting for age, race, and other confounding factors, meta-analysis results showed that vitamin D deficiency in the first trimester, the second trimester, and the third trimester did not increase the risk of PTB (odds ratio (OR) = 1.01, 95% confidence interval (CI) (0.88, 1.16), P = .867; OR = 1.12, 95%CI (0.92, 1.37), P = .249; OR = 1.05, 95%CI (0.87, 1.27), P = .602). However, there was moderate heterogeneity in the study of vitamin D deficiency in the second trimester, and subgroup analysis suggested that vitamin D deficiency in the second trimester may increase the risk of PTB (OR = 1.33, 95%CI (1.15, 1.54), P = .000). A sensitivity analysis of the second trimester showed that excluding any 1 study did not significantly change the results.

Conclusions: Vitamin D deficiency in early and late pregnancy may not be associated with PTB, while vitamin D deficiency in middle pregnancy is likely to have an important effect on PTB. Vitamin D levels should be measured in the second trimester of pregnancy, and vitamin D supplements should be provided if necessary.
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http://dx.doi.org/10.1097/MD.0000000000026303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213249PMC
June 2021

Long non-coding RNA AFAP1-AS1 facilitates prostate cancer progression by regulating miR-15b/IGF1R axis.

Curr Pharm Des 2021 Jun 11. Epub 2021 Jun 11.

Department of Urology, Tongji Hospital, Tongji University of Medicine, 389 Xincun Road, Putuo, Shanghai, 200065, China.

Background: Prostate cancer (PCa) is a commonly diagnosed malignant cancer and is the second highest cause of cancer related death in men worldwide. Enzalutamide is the second-generation inhibitor of androgen receptor signaling and is the fundamental drug for the treatment of advanced PCa. However, the disease will eventually progress to metastatic castration-resistant prostate cancer (CRPC) and aggressive neuroendocrine prostate cancer (NEPC) because of androgen-deprivation therapy (ADT) resistance. The aim of the study was to investigate the role of long non-coding RNA (lncRNA) AFAP1-AS1 in ADT resistance.

Methods: Quantitative real-time PCR analysis (qPCR) was used to assess the expression of AFAP1-AS1 in PCa cell lines and tissues. Cell proliferation and invasion were assessed after AFAP1-AS1 knockdown using Cell Counting Kit (CCK)-8 and Transwell assay, respectively. A dual-luciferase reporter gene assay was carried out to validate the regulatory relationship among AFAP1-AS1, microRNA (miR)-15b, and insulin-like growth factor1 receptor (IGF1R).

Results: AFAP1-AS1 level was markedly increased in castration-resistant C4-2 cells and NE-like cells (PC3, DU145, and NCI-H660), compared with androgen-sensitive LNCaP cells. Enzalutamide treatment increased the expression of AFAP1-AS1 in vitro and in vivo. Functionally, AFAP1-AS1 knockdown repressed tumor cell proliferation and invasion. Mechanistically, AFAP1-AS1 functioned as an oncogene in PCa through binding to miR-15b and destroying its tumor suppressor function. Finally, we identified that AFAP1-AS1 up-regulated IGF1R expression by competitively binding to miR-15b to de-repress IGF1R.

Conclusion: AFAP1-AS1 facilitates PCa progression by regulating miR-15b/IGF1R axis, indicating that AFAP1-AS1 may serve as a diagnostic biomarker and therapeutic target for PCa.
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http://dx.doi.org/10.2174/1381612827666210612052317DOI Listing
June 2021

Bovine β-Lactoglobulin Covalent Modification by Flavonoids: Effect on the Allergenicity and Human Intestinal Microbiota.

J Agric Food Chem 2021 Jun 9;69(24):6820-6828. Epub 2021 Jun 9.

National R&D Center for Freshwater Fish Processing, and Engineering Research Center of Freshwater Fish High-value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.

The present study aims to investigate the structure of covalent conjugates of bovine β-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and -phthaldialdehyde assay. The secondary and conformational structures of BLG were changed by the covalent modification, which were determined by the circular dichroism, Fourier transform infrared spectroscopy, fluorescence spectroscopy, and UV spectroscopy. The enzyme-linked immunosorbent assay (ELISA) and cell experiments indicated that BLG covalent conjugates could reduce IgE/IgG binding capacities and suppress the allergy reactivity of RBL-2H3 cells, suggesting that the covalent modification modulated the balance of T cells. Meanwhile, covalent modification of BLG with these flavonoids can alter the diversity of human intestinal microbiota and the community abundance at phylum, family, and genus levels. The results revealed that covalent modification of BLG with flavonoids alters human intestinal microbiota, might result in the reduction of allergenicity, which could provide information for confirming the relationship between food allergy and the intestinal microbial ecosystem.
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http://dx.doi.org/10.1021/acs.jafc.1c02482DOI Listing
June 2021

Integrated mRNA and small RNA sequencing reveals a regulatory network associated with flower color in oriental hybrid lily.

Plant Physiol Biochem 2021 Sep 28;166:103-114. Epub 2021 May 28.

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing, China. Electronic address:

Anthocyanins are one of the main components of pigments, that are responsible for a wide range of colors in plants. To clarify the regulatory mechanism of anthocyanin biosynthesis in oriental hybrid lily, UPLC/MS analysis was performed to identify the pigments in two cultivars (white and pink). Four major anthocyanins were identified in pink cultivar, and no anthocyanins were detected in white cultivar. Transcriptome and small RNA sequencing (sRNAseq) analyses were performed using tepal tissues at two floral developmental stages from the two cultivars. In total, 55,698 transcripts were assembled, among which 233 were annotated as putative anthocyanin-related transcripts. Differential expression analysis and qRT-PCR results confirmed that most of the anthocyanin-related structural genes had higher expression levels in pink cultivar than in white cultivar. Conversely, LhANR showed a significantly high expression level in white cultivar. Annotated transcription factors (TFs), including MYB activators, MYB repressors and bHLHs, that putatively inhibit or enhance the expression of anthocyanin-related genes were identified. LhMYBA1, an anthocyanin activator, was isolated, and its heterologous expression resulted in a remarkably high level of anthocyanin accumulation. Additionally, 73 differentially expressed microRNAs (miRNAs), including 23 known miRNAs, were detected through sRNAseq. The miRNA target prediction showed that several anthocyanin-related genes might be targeted by miRNAs. Expression profile analysis revealed that these miRNAs showed higher expression levels at later floral developmental stages in white cultivar than in pink cultivar. The results indicated that anthocyanin deficiency in white cultivar might be influenced by multiple levels of suppressive mechanisms, including mRNAs and sRNAs.
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http://dx.doi.org/10.1016/j.plaphy.2021.05.035DOI Listing
September 2021

Ceramic boron carbonitrides for unlocking organic halides with visible light.

Chem Sci 2021 Mar 23;12(18):6323-6332. Epub 2021 Mar 23.

State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University Fuzhou 350116 China

Photochemistry provides a sustainable pathway for organic transformations by inducing radical intermediates from substrates through electron transfer process. However, progress is limited by heterogeneous photocatalysts that are required to be efficient, stable, and inexpensive for long-term operation with easy recyclability and product separation. Here, we report that boron carbonitride (BCN) ceramics are such a system and can reduce organic halides, including (het)aryl and alkyl halides, with visible light irradiation. Cross-coupling of halides to afford new C-H, C-C, and C-S bonds can proceed at ambient reaction conditions. Hydrogen, (het)aryl, and sulfonyl groups were introduced into the arenes and heteroarenes at the designed positions by means of mesolytic C-X (carbon-halogen) bond cleavage in the absence of any metal-based catalysts or ligands. BCN can be used not only for half reactions, like reduction reactions with a sacrificial agent, but also redox reactions through oxidative and reductive interfacial electron transfer. The BCN photocatalyst shows tolerance to different substituents and conserved activity after five recycles. The apparent metal-free system opens new opportunities for a wide range of organic catalysts using light energy and sustainable materials, which are metal-free, inexpensive and stable.
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http://dx.doi.org/10.1039/d1sc01028jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115245PMC
March 2021

New Enhancement beyond Radiation Field Improves Survival Prediction in Patients with Post-Treatment High-Grade Glioma.

J Oncol 2021 5;2021:9437090. Epub 2021 May 5.

Department of Medical Imaging, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

The imaging signs which can accurately predict survival prognosis after standard treatment of high-grade glioma (HGG) are highly desirable. This study aims to explore the role of new enhancement beyond radiation field (NERF) in the survival prediction in patients with post-treatment HGG. The present study included 142 pathologically confirmed HGG patients who had received standard treatment. NERF, as well as other conventional MR findings and clinical variables, were included in univariate and multivariate analyses for evaluating their impactions on progression-free survival (PFS) and overall survival (OS). Univariate analysis showed that histological grade (=0.008) and NERF (=0.001) were the prognostic variables for poor PFS, whereas histological grade (=0.017), NERF (=0.001), and new subventricular zone enhancement (nSVZE) (=0.001) were prognostic variables for poor OS. The multivariate analysis showed that NERF (HR 3.93; 95% CI 1.93-8.01; =0.001) and nSVZE (HR 3.92; 95% CI 1.95-7.89; =0.001) were the prognostic variables for poor OS. However, only nSVZE was (HR 3.29; 95% CI 2.04-5.28; =0.001) the prognostic variable for poor PFS. When combining the NERF with the clinical and other MR variables, the highest AUC (0.924) and specificity (0.899) for predicting poor OS were achieved. The location of new developed enhancements relevant to high dose radiation field appears to be the main determinant of their prognostic value. Our results suggest that the new enhancement beyond radiation field can improve the survival prediction in patients with HGG after standard treatment.
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http://dx.doi.org/10.1155/2021/9437090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118721PMC
May 2021
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