Publications by authors named "Yuan Rui"

140 Publications

HIF-1α and HIF-2α redundantly promote retinal neovascularization in patients with ischemic retinal disease.

J Clin Invest 2021 Jun;131(12)

Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Therapies targeting VEGF have proven only modestly effective for the treatment of proliferative sickle cell retinopathy (PSR), the leading cause of blindness in patients with sickle cell disease. Here, we shift our attention upstream from the genes that promote retinal neovascularization (NV) to the transcription factors that regulate their expression. We demonstrated increased expression of HIF-1α and HIF-2α in the ischemic inner retina of PSR eyes. Although both HIFs participated in promoting VEGF expression by hypoxic retinal Müller cells, HIF-1 alone was sufficient to promote retinal NV in mice, suggesting that therapies targeting only HIF-2 would not be adequate to prevent PSR. Nonetheless, administration of a HIF-2-specific inhibitor currently in clinical trials (PT2385) inhibited NV in the oxygen-induced retinopathy (OIR) mouse model. To unravel these discordant observations, we examined the expression of HIFs in OIR mice and demonstrated rapid but transient accumulation of HIF-1α but delayed and sustained accumulation of HIF-2α; simultaneous expression of HIF-1α and HIF-2α was not observed. Staggered HIF expression was corroborated in hypoxic adult mouse retinal explants but not in human retinal organoids, suggesting that this phenomenon may be unique to mice. Using pharmacological inhibition or an in vivo nanoparticle-mediated RNAi approach, we demonstrated that inhibiting either HIF was effective for preventing NV in OIR mice. Collectively, these results explain why inhibition of either HIF-1α or HIF-2α is equally effective for preventing retinal NV in mice but suggest that therapies targeting both HIFs will be necessary to prevent NV in patients with PSR.
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http://dx.doi.org/10.1172/JCI139202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203455PMC
June 2021

Effects of Hsp90 Inhibitor Ganetespib on Inhibition of Azole-Resistant .

Front Microbiol 2021 20;12:680382. Epub 2021 May 20.

School of Pharmacy, Second Military Medical University, Shanghai, China.

is the most common fungal pathogen. Recently, drug resistance of is increasingly severe. Hsp90 is a promising antifungal target to overcome this problem. To evaluate the effects of Hsp90 inhibitor ganetespib on the inhibition of azole-resistant , the microdilution checkerboard method was used to measure the synergistic efficacy of ganetespib. The XTT/menadione reduction assay, microscopic observation, and Rh6G efflux assay were established to investigate the effects of ganetespib on azole-resistant biofilm formation, filamentation, and efflux pump. Real-time RT-PCR analysis was employed to clarify the mechanism of antagonizing drug resistance. The antifungal efficacy of ganetespib was determined by the infectious model of azole-resistant . Ganetespib showed an excellent synergistic antifungal activity and significantly inhibited the fungal biofilm formation, whereas it had no inhibitory effect on fungal hypha formation. Expression of azole-targeting enzyme gene and efflux pump genes , and was significantly down-regulated when ganetespib was used in combination with FLC. In a mouse model infected with FLC-resistant , the combination of ganetespib and FLC effectively reversed the FLC resistance and significantly decreased the kidney fungal load of mouse.
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http://dx.doi.org/10.3389/fmicb.2021.680382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174564PMC
May 2021

Metatranscriptomic Analysis Reveals an Imbalance of Hepatopancreatic Flora of Chinese Mitten Crab with Hepatopancreatic Necrosis Disease.

Biology (Basel) 2021 May 23;10(6). Epub 2021 May 23.

School of Biology and Basic Medical Science, Soochow University, Suzhou 215123, China.

Hepatopancreas necrosis disease (HPND) of the Chinese mitten crab Eriocheir sinensis causes huge economic loss in China. However, the pathogenic factors and pathogenesis are still a matter of dissension. To search for potential pathogens, the hepatopancreatic flora of diseased crabs with mild symptoms, diseased crabs with severe symptoms, and crabs without visible symptoms were investigated using metatranscriptomics sequencing. The prevalence of Absidia glauca and Candidatus Synechococcus spongiarum decreased, whereas the prevalence of Spiroplasma eriocheiris increased in the hepatopancreatic flora of crabs with HPND. Homologous sequences of 34 viral species and 4 Microsporidian species were found in the crab hepatopancreas without any significant differences between crabs with and without HPND. Moreover, DEGs in the hepatopancreatic flora between crabs with severe symptoms and without visible symptoms were enriched in the ribosome, retinol metabolism, metabolism of xenobiotics by cytochrome P450, drug metabolism-cytochrome P450, biosynthesis of unsaturated fatty acids, and other glycan degradation. Moreover, the relative abundance of functions of DEDs in the hepatopancreatic flora changed with the pathogenesis process. These results suggested that imbalance of hepatopancreatic flora was associated with crab HPND. The identified DEGs were perhaps involved in the pathological mechanism of HPND; nonetheless, HPND did not occur due to virus or microsporidia infection.
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http://dx.doi.org/10.3390/biology10060462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224665PMC
May 2021

Overcoming delivery barriers in immunotherapy for glioblastoma.

Drug Deliv Transl Res 2021 May 30. Epub 2021 May 30.

Department of Biomedical Engineering, the Institute for Nanobiotechnology and the Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Glioblastoma is one of the deadliest forms of primary adult tumors, with median survival of 14.6 months post-diagnosis despite aggressive standard of care treatment. This grim prognosis for glioblastoma patients has changed little in the past two decades, necessitating novel treatment modalities. One potential treatment modality is cancer immunotherapy, which has shown remarkable progress in slowing disease progression or even potentially curing certain solid tumors. However, the transport barriers posed by the blood-brain barrier and the immune privileged status of the central nervous system pose drug delivery obstacles that are unique to brain tumors. In this review, we provide an overview of the various physiological, immunological, and drug delivery barriers that must be overcome for effective glioblastoma treatment. We discuss chemical modification strategies to enable nanomedicines to bypass the blood-brain barrier and reach intracranial tumors. Finally, we highlight recent advances in biomaterial-based strategies for cancer immunotherapy that can be adapted to glioblastoma treatment.
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http://dx.doi.org/10.1007/s13346-021-01008-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164566PMC
May 2021

Proteinuria may be an indicator of adverse pregnancy outcomes in patients with preeclampsia: a retrospective study.

Reprod Biol Endocrinol 2021 May 14;19(1):71. Epub 2021 May 14.

The Center of Experimental Teaching Management, Chongqing Medical University, Chongqing, China.

Background: Proteinuria is one of the common manifestations of patients with preeclampsia (PE), but whether the severity of proteinuria is related to the pregnancy outcome of patients with preeclampsia remains controversial. The present study aimed to determine the relationship between 24-h proteinuria and adverse outcomes in patients with preeclampsia.

Methods: The present retrospective study included 329 pregnant women in Chongqing, China. Patients were divided into PE group and non-PE group. PE group was stratified into three subgroups based on the level of 24-h proteinuria. Correlation analysis was used to analyze the correlation between biochemical indexes and adverse pregnancy outcome, and Logistic regression analysis was used to analyze the risk factors of adverse pregnancy outcome. The receiver operating characteristic curve (ROC) was used to evaluate the ability of 24-h urinary protein to distinguish the adverse pregnancy outcome in patients with preeclampsia.

Results: (1) Between PE and non-PE group, cesarean section rate in PE group was significantly higher than that in non-PE group (84.4% vs. 25.9%, p <  0.001). Laboratory findings such as uric acid and creatinine level in PE group were higher than those in non-PE group. (2) Among mild (proteinuria < 0.3 g/24 h), moderate (0.3 g/24 h ≦ proteinuria < 2 g/24 h) and massive (proteinuria ≧ 2 g/24 h) groups, the frequencies of induced labor (p = 0.006) and stillbirth (p = 0.002) increased with the increase of 24-h proteinuria. (3) Adverse outcomes were positively correlated with 24-h proteinuria (adverse maternal outcomes: r = 0.239, p = 0.002; adverse fetal outcomes: r = 0.336, p <  0.001). (4) The best 24-h proteinuria cutoff values to determine stillbirth, premature and fetal distress were 3965.0 mg/24 h, 984.75 mg/24 h and 1503.85 mg/24 h and their odds ratio (95% confidence interval) were 12.46 (3.46-44.88), 2.48 (1.15-5.37) and 10.02 (2.14-46.80), respectively.

Conclusions: The severity of 24-h proteinuia may forecast adverse outcomes in women with preeclampsia. We suggest proteinuria should be retained as one of the monitoring indexes in patients with preeclampsia.

Trial Registration: Retrospectively registered. (LTMCMTS202001).
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http://dx.doi.org/10.1186/s12958-021-00751-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120921PMC
May 2021

Incidence of Gibelio Gill Hemorrhagic Disease Caused by CyHV-2 Infection Can Be Reduced by Vaccination with Polyhedra Incorporating Antigens.

Vaccines (Basel) 2021 Apr 16;9(4). Epub 2021 Apr 16.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

Encapsulation of antigens within protein microcrystals (polyhedra) is a promising approach for the stable delivery of vaccines. In this study, a vaccine was encapsulated into polyhedra against cyprinid herpesvirus II (CyHV-2). CyHV-2 typically infects gibel carp, gibelio, causing gill hemorrhagic disease. The vaccine was constructed using a codon-optimized sequence, D4ORF, comprising the ORF72 (region 1-186 nt), ORF66 (region 993-1197 nt), ORF81 (region 603-783 nt), and ORF82 (region 85-186 nt) genes of CyHV-2. The H1-D4ORF and D4ORF-VP3 sequences were, respectively, obtained by fusing the H1-helix sequence (region 1-90 nt) ofBombyx mori cypovirus(BmCPV) polyhedrin to the 5' terminal end of D4ORF and by fusing a partial sequence (1-279 nt) of the BmCPV VP3 gene to the 3' terminal end of D4ORF. Furthermore, BmNPV-H1-D4ORF-polh and BmNPV-D4ORF-VP3-polh recombinant nucleopolyhedroviruses (BmNPVs), belonging to the family Baculoviridae, and co-expressing BmCPV polyhedrin and H1-D4ORF or D4ORF-VP3, were constructed. H1-D4ORF and D4ORF-VP3 fusion proteins were confirmed to be encapsulated into recombinant cytoplasmic polyhedra by Western blotting. Degradation of vaccine proteins was assessed by SDS-PAGE, and the results showed that the encapsulated vaccine proteins in polyhedra could be protected from degradation. Furthermore, when gibel carp were vaccinated with the purified polyhedra from BmNPV-H1-D4ORF-polh and BmNPV-D4ORF-VP3-polh via injection, the antibody titers in the serum of the vaccinated fish reached 1:6400-1:12,800 at 3 weeks post-vaccination. Therelative percentage of survival of immunized gibel carp reached 64.71% and 58.82%, respectively, following challenge with CyHV-2. These results suggest that incorporating vaccine protein into BmCPV polyhedra may be a novel approach for developing aquaculture microencapsulated vaccines.
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http://dx.doi.org/10.3390/vaccines9040397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072653PMC
April 2021

Substituents and the induced partial charge effects on cobalt porphyrins catalytic oxygen reduction reactions in acidic medium.

J Colloid Interface Sci 2021 Sep 8;597:269-277. Epub 2021 Apr 8.

Department of Chemistry, Tsinghua University, Beijing 100084, PR China. Electronic address:

Charge states at the catalytic interface can intensely alter the charge transfer mechanism and thus the oxygen reduction performance. Two symmetric cobalt porphyrins with electron deficient 2,1,3-benzothiadiazole (BTD) and electron-donating propeller-like triphenylamine (TPA) derivatives have been designed firstly, to rationally generate intramolecular partial charges, and secondly, to utilize the more exposed molecular orbitals on TPA for enhancing the charge transfer kinetics. The catalytic performance of the two electrocatalysts was examined for oxygen reduction reactions (ORR) in acidic electrolyte. It was found that BCP1/C with two BTD groups showed greater reduction potential but less limiting current density as compared to BCP2/C bearing BTD-TPA units. The reduced potential of BCP2/C was proposed to the introduction of the electron-donating ability of TPA, which may decrease the adsorption affinity of oxygen to the cobalt center. Both dipole-induced partial charge effect and the more exposed cation orbitals of the 3D structural TPA were proposed to contribute to the increased response current of BCP2/C. In addition, BCP2/C attained more than 80% of HO generation in acidic solution, which may also relate to the structural effect. These findings may provide new insight into the structural design of organic electrocatalysts and deep understanding on the interfacial charge transfer mechanism for ORR.
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http://dx.doi.org/10.1016/j.jcis.2021.04.009DOI Listing
September 2021

Flipped Quick-Response Code Enables Reliable Blood Grouping.

ACS Nano 2021 04 19;15(4):7649-7658. Epub 2021 Apr 19.

Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044, People's Republic of China.

Accurate and rapid blood typing plays a vital role in a variety of biomedical and forensic scenarios, but recognizing weak agglutination remains challenging. Herein, we demonstrated a flipping identification with a prompt error-discrimination (FLIPPED) platform for automatic blood group readouts. Bromocresol green dye was exploited as a characteristic chromatography indicator for the differentiation of plasma from whole blood by presenting a teal color against a brown color. After integrating these color changes into a quick-response (QR) code, prompt typing of ABO and Rhesus groups was automatically achieved and data could be uploaded wirelessly within 30 s using a commercially available smartphone to facilitate blood cross-matching. We further designed a color correction model and algorithm to remove potential errors from scanning angles and ambient light intensities, by which weak agglutination could be accurately recognized. With comparable accuracy and repeatability to classical column assay in grouping 450 blood samples, the proposed approach further demonstrates to be a versatile sample-to-result platform for clinical diagnostics, food safety, and environmental monitoring.
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http://dx.doi.org/10.1021/acsnano.1c01215DOI Listing
April 2021

Correction to: Establishment and effectiveness evaluation of a scoring system for exertional heat stroke by retrospective analysis.

Mil Med Res 2021 Apr 16;8(1):26. Epub 2021 Apr 16.

Department of Critical Care Medicine, The First Medical Centre, Chinese PLA General Hospital, No. 28, Fuxing Road, Haidian District, Beijing, 100853, China.

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http://dx.doi.org/10.1186/s40779-021-00318-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052645PMC
April 2021

Long-term effects of intermittent early life stress on primate prefrontal-subcortical functional connectivity.

Neuropsychopharmacology 2021 06 25;46(7):1348-1356. Epub 2021 Jan 25.

Department of Psychiatry and Behavioral Sciences, Stanford, USA.

Correlational studies of humans suggest that exposure to early life stress has long-term effects on neural circuits involved in vulnerability and resilience to mental health disorders. Stress-related mental health disorders are more prevalent in women than in men. Here, female squirrel monkeys are randomized to intermittently stressful (IS) social separations or a non-separated (NS) control condition conducted from 17 to 27 weeks of age. Nine years later in mid-life adulthood, resting-state functional magnetic resonance imaging was employed to parcellate prefrontal cortex (PFC). Resulting subdivisions were then used to characterize functional connectivity within PFC, and between PFC subdivisions and subcortical regions that are known to be altered by stress. Extensive hyper-connectivity of medial and orbitofrontal PFC with amygdala, hippocampus, and striatum was observed in IS compared to NS monkeys. Functional hyper-connectivity in IS monkeys was associated with previously reported indications of diminished anxiety-like behavior induced by prepubertal stress. Hyper-connectivity of PFC with amygdala and with hippocampus was also associated with increased ventral striatal dopamine D2 and/or D3 receptor (DRD2/3) availability assessed with positron emission tomography (PET) of [C]raclopride binding in adulthood. Ventral striatal DRD2/3 availability has been linked to cognitive control, which plays a key role in stress coping as an aspect of emotion regulation. These findings provide causal support for enduring neurobiological effects of early life stress and suggest novel targets for new treatments of stress-related mental health disorders.
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http://dx.doi.org/10.1038/s41386-021-00956-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134590PMC
June 2021

Long-term effects of intermittent early life stress on primate prefrontal-subcortical functional connectivity.

Neuropsychopharmacology 2021 06 25;46(7):1348-1356. Epub 2021 Jan 25.

Department of Psychiatry and Behavioral Sciences, Stanford, USA.

Correlational studies of humans suggest that exposure to early life stress has long-term effects on neural circuits involved in vulnerability and resilience to mental health disorders. Stress-related mental health disorders are more prevalent in women than in men. Here, female squirrel monkeys are randomized to intermittently stressful (IS) social separations or a non-separated (NS) control condition conducted from 17 to 27 weeks of age. Nine years later in mid-life adulthood, resting-state functional magnetic resonance imaging was employed to parcellate prefrontal cortex (PFC). Resulting subdivisions were then used to characterize functional connectivity within PFC, and between PFC subdivisions and subcortical regions that are known to be altered by stress. Extensive hyper-connectivity of medial and orbitofrontal PFC with amygdala, hippocampus, and striatum was observed in IS compared to NS monkeys. Functional hyper-connectivity in IS monkeys was associated with previously reported indications of diminished anxiety-like behavior induced by prepubertal stress. Hyper-connectivity of PFC with amygdala and with hippocampus was also associated with increased ventral striatal dopamine D2 and/or D3 receptor (DRD2/3) availability assessed with positron emission tomography (PET) of [C]raclopride binding in adulthood. Ventral striatal DRD2/3 availability has been linked to cognitive control, which plays a key role in stress coping as an aspect of emotion regulation. These findings provide causal support for enduring neurobiological effects of early life stress and suggest novel targets for new treatments of stress-related mental health disorders.
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http://dx.doi.org/10.1038/s41386-021-00956-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134590PMC
June 2021

Effect of the Information Support Method Combined with Yoga Exercise on the Depression, Anxiety, and Sleep Quality of Menopausal Women.

Authors:
Xi Lu Lu Liu Rui Yuan

Psychiatr Danub 2020 Autumn - Winter;32(3-4):380-388

Department of Public Physical and Art Education, Zhejiang University, Hanzhou 310000, China,

Background: Menopause is a special stage in a woman's life, but no safe clinical treatment exists against menopausal symptoms. To analyze the effect of the information support method combined with yoga exercise on the depression, anxiety, and sleep quality of menopausal women.

Subjects And Methods: From June 2019 to December 2019, menopausal women who were newly recruited in three yoga clubs in three cities in East China were selected as the participants by convenience sampling. A total of 52 women were in the experiment group and 54 were in the control group. In 24 weeks, the experiment group engaged in yoga exercise for 60 minutes each time, three times a week. They group was given professional positive psychological information support at the same time. The Kupperman Menopausal Symptom Distress Scale, Self-rating Depression Scale (SDS), Self-rating Anxiety Scale (SAS), and Pittsburgh Sleep Quality Index (PSQI) were used before the experiment, three months into the experiment, and six months into the experiment to monitor the intervention effect on the participants.

Results: After the intervention, the symptoms of distress among menopausal women in the experiment group and the Kupperman score of the experiment group reduced significantly. Repeated measures of analysis of variance were conducted in the two groups (P<0.001). After the intervention, the depression score of the experiment group decreased significantly. A significant difference was found between the two groups in repeated measures analysis of variance in the SDS score (P<0.001). After the intervention, the anxiety score of the experiment group reduced significantly, and repeated measures of analysis of variance in the SAS score were conducted in the two groups (P<0.001). After the intervention, the sleep quality of the experiment group improved, and repeated measures of analysis of variance in sleep quality were conducted in the two groups (P<0.001).

Conclusions: The information support method combined with yoga exercise can alleviate the depression and anxiety of menopausal women, improve their sleep quality, and reduce their symptoms of menopausal distress.
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http://dx.doi.org/10.24869/psyd.2020.380DOI Listing
January 2021

DiR-labeled tolerogenic dendritic cells for targeted imaging in collagen- induced arthritis rats.

Int Immunopharmacol 2021 Feb 24;91:107273. Epub 2020 Dec 24.

School of Clinical Laboratory Science, Guizhou Medical University, Guiyang 550000, Guizhou, China; Department of Microbiology and Immunology, Affiliated Hospital of Guizhou Medical University, Guiyang 550000, Guizhou, China. Electronic address:

Tolerogenic dendritic cells (tolDCs) are immunosuppressive cells and play an important role in rheumatoid arthritis (RA) as immunotherapeutic tools. We aimed to investigate whether allogeneic tolDCs (allo-tolDCs) and autologous tolDCs (auto-tolDCs) had long-time tolerogenic potential in vivo and improve arthritis in collagen-induced arthritis (CIA) rats. TolDCs were induced by NF-κB Decoy ODN, and loaded with Bovine Type II collagen (CII- loaded tolDCs) and identified by flow cytometry, and labeled with DiR and injected into CIA rats. The biodistribution of DiR-labeled tolDCs was monitored by IVIS imaging at different time points. Major organs were harvested and analyzed by ex-in vivo cell imaging. The tolDCs were successfully constructed, along with expressing low levels of CD80 and CD86 compared to DCs. The fluorescent signals of all DiR (+) groups were observed at least 25 days, and as long as 35 days. DiR (+) CII- loaded allo-and auto-tolDCs at post injection mainly distributed in the chest and abdomen and gradually moved to limb joints over time. The allo- and auto-tolDCs decreased the expression of IFN-γ and IL-2 in CIA rats with different severity compared to CIA rats without tolDCs treatment, while significantly increased the expression of IL-4 and IL-10. Additionally, these tolDCs ameliorated the ankle joints injury in CIA rats with different severity. The both allo- and auto-tolDCs showed long-time tolerogenic potential in vivo and ameliorated arthritis in CIA rats with different severity.
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http://dx.doi.org/10.1016/j.intimp.2020.107273DOI Listing
February 2021

Bone marrow mesenchymal stem cell-derived exosomes alleviate hyperoxia-induced lung injury via the manipulation of microRNA-425.

Arch Biochem Biophys 2021 01 29;697:108712. Epub 2020 Nov 29.

Department of Thoracic Surgery/Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China. Electronic address:

Background: Hyperoxia-induced lung injury (HILI) is an acute lung injury (LI) induced by extended periods of exposure to hyperoxia. Alleviating LI by bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos) and microRNAs (miRs) has been previously reported. This study is devised to probe the interaction between BMSCs-Exos and miR-425 in HILI.

Methods: Firstly, BMSCs-Exos were isolated and identified. Then, HILI rat models and RLE-6TN cell models were successfully established and treated by BMSCs-Exos. Afterwards, functional assays were conducted to explore cell biological behaviors in models, with miR-425 expression detected. Then, the target relation between miR-425 and PTEN was clarified by luciferase reporter assay. Eventually, expression of PTEN and the PI3K/Akt axis was assessed by Western blotting and qRT-PCR.

Results: BMSCs-Exos promoted miR-425 expression and attenuated HILI and HO induced RLE-6TN cell injury as evidence by alleviated lung cell injury, decreased TUNEL-positive cells, induced cell viability and declined apoptosis (all p < 0.05). Besides, when miR-425 was knocked-down, the protective role of BMSCs-Exos in HILI was also reduced (all p < 0.05). miR-425 targeted PTEN mRNA, whose upregulation reversed the protective role of BMSCs-Exos in HILI (all p < 0.05). BMSCs-Exos improved the quenched levels of the PI3K/AKT axis in HILI (all p < 0.05).

Conclusion: Our data supported that miR-425 in BMSCs-Exos inhibits HILI by targeting PTEN and upregulating the PI3K/AKT axis. This study may provide personalized interventions for HILI remedy.
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http://dx.doi.org/10.1016/j.abb.2020.108712DOI Listing
January 2021

Application of large-scale targeted sequencing to distinguish multiple lung primary tumors from intrapulmonary metastases.

Sci Rep 2020 11 2;10(1):18840. Epub 2020 Nov 2.

The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.

The effective differentiation between multiple primary lung tumors (MPs) and intrapulmonary metastases (IMs) in patients is imperative to discover the exact disease stage and to select the most appropriate treatment. In this study, the authors was to evaluate the efficacy and validity of large-scale targeted sequencing (LSTS) as a supplement to estimate whether multifocal lung cancers (MLCs) are primary or metastatic. Targeted sequencing of 520 cancer-related oncogenes was performed on 36 distinct tumors from 16 patients with MPs. Pairing analysis was performed to evaluate the somatic mutation pattern of MLCs in each patient. A total of 25 tumor pairs from 16 patients were sequenced, 88% (n = 22) of which were classified as MPs by LSTS, consistent with clinical diagnosis. One tumor pair from a patient with lymph node metastases had highly consistent somatic mutation profiles, thus predicted as a primary-metastatic pair. In addition, some matched mutations were observed in the remaining two paired ground-glass nodules (GGNs) and classified as high-probability IMs by LSTS. Our study revealed that LSTS can potentially facilitate the distinction of MPs from IMs. In addition, our results provide new genomic evidence of the presence of cancer invasion in GGNs, even pure GGNs.
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http://dx.doi.org/10.1038/s41598-020-75935-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606457PMC
November 2020

High-Yield Production of Water-Soluble MoS Quantum Dots for Fe Detection and Cell Imaging.

Nanomaterials (Basel) 2020 Oct 29;10(11). Epub 2020 Oct 29.

Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Uniform water-soluble MoS quantum dots (WS-MSQDs) were synthesized via a sequential combination of sintering/etching/exfoliation method and solvothermal route. The obtained WS-MSQDs with average size of approximately 3.4 nm exhibited sufficient water solubility and remarkable fluorescence properties. The WS-MSQDs were utilized as a probe for detection of Fe ions with high selectivity and specificity. Furthermore, the WS-MSQDs exhibited high fluorescence stability under different conditions. Finally, the WS-MSQDs were successfully applied for the fluorescence imaging of Fe in living cells, which exhibited practical potential for biomedical applications.
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http://dx.doi.org/10.3390/nano10112155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692859PMC
October 2020

Baseline-Free Adaptive Crack Localization for Operating Stepped Rotors Based on Multiscale Data Fusion.

Sensors (Basel) 2020 Oct 6;20(19). Epub 2020 Oct 6.

Hubei Key Laboratory of Mechanical Transmission and Manufacturing Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

Crack localization in running rotors is very important and full of challenges for machinery operation and maintenance. Characteristic deflection shapes or their derivatives based methods seem to be promising for crack localization in rotors. Despite the substantial advantages, several critical issues still need to be addressed to enhance the efficiency of this kind of method for practical applications. Two problems are considered in this work: 1. How to localize single or multiple cracks accurately avoiding the interference of commonly existing steps without baseline information on pristine rotors; 2. How to improve the crack localization performance under a noisy environment. To circumvent the issues, a novel baseline-free adaptive crack localization method is proposed based on data fusion of multiscale super-harmonic characteristic deflection shapes (SCDSs). In this method, crack induced asymmetry and nonlinearity of crack breathing are utilized to simultaneously eliminate the interference from the steps without a reference model. To enhance the noise robustness, the multiscale representations of SCDSs are made in Gaussian multiscale space, and Teager energy operator is applied to the multiscale SCDSs to amplify the crack induced singularities and construct the multiscale Teager super-harmonic characteristic deflection shapes (TSCDSs). Moreover, fractal dimension is designed as an evaluator to select the proper multiscale TSCDSs for data fusion adaptively. Then, a new damage index is derived for crack localization by Dempster-Shafer's (D-S) evidence fusion of the adaptively selected multiscale TSCDSs. Finally, the feasibility and the effectiveness are verified by both numerical and experimental investigations.
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http://dx.doi.org/10.3390/s20195693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582652PMC
October 2020

Fabrication and Corrosion Resistance of a Superhydrophobic Ni-P/Ni(NO)(OH) Multilayer Protective Coating on Magnesium Alloy.

ACS Omega 2020 Sep 18;5(38):24247-24255. Epub 2020 Sep 18.

College of Physics Electronic Information Engineering, Qinghai University for Nationalities, Xining, Qinghai 810007, P R China.

A superhydrophobic multilayer coating with excellent corrosion resistance has been fabricated on an AZ61 magnesium alloy through electroless plating and hydrothermal treatment. The surface morphologies, chemical characteristics, wettabilities, and corrosion resistance of the multilayer coating were characterized and discussed. The results show that the electroless Ni-P coating on the magnesium alloy exhibits a nodular structure with micropores and lower corrosion resistance. However, a dense Ni(NO)(OH) layer, a porous Ni(NO)(OH) nanostructure layer, and a stearic absorbing layer are grown on the surface of the Ni-P coating with superhydrophobic characters and higher corrosion resistance after hydrothermal treatment. Furthermore, the water contact angle and corrosion resistance of the multilayer coating showed a trend of first increasing and then decreasing as the hydrothermal reaction time increases. The optimum hydrothermal reaction time is 15 h, and the multilayer coating prepared under this condition has the highest corrosion resistance and the highest contact angle. In addition, the protection mechanism of the multilayer coating is discussed, and the formation of the dense Ni(NO)(OH) layer and the stearic absorbing layer effectively improved the corrosion resistance of the multilayer coating.
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http://dx.doi.org/10.1021/acsomega.0c02196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528178PMC
September 2020

Schisandra Fruit Vinegar Lowers Lipid Profile in High-Fat Diet Rats.

Evid Based Complement Alternat Med 2020 3;2020:7083415. Epub 2020 Sep 3.

Department of Pharmacology, College of Pharmacy, Beihua University, Jilin 132013, China.

Hyperlipidemia and its associated obesity, hepatic steatosis, and NAFLD are worldwide problems. However, there is no ideal pharmacological treatment for these. Therefore, the complementary therapies that are both natural and safe have been focused. Healthy foods, such as fruit vinegar, may be one of the best choices. In this study, we made a special medicinal fruit vinegar, Schisandra fruit vinegar (SV), and examined its lipid-lowering effects and the underlying mechanisms in a high-fat diet rat model. The results showed that SV significantly reduced the body weight, liver weight, liver index, the serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), free fatty acid (FFA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), increased the content of high-density lipoprotein cholesterol (HDL-C) and the activity of superoxide dismutase (SOD), upregulated the expressions of peroxisome proliferative activated receptor (PPAR-), peroxisomal acyl-coenzyme A oxidase 1 (ACOX1), and carnitine palmitoyltransferase 1 (CPT1) proteins, increased the contents of key component of antioxidant defense NF-E2-related factor 2 (NRF2) and its downstream heme oxygenase-1 (HO-1) protein, and downregulated the expression of Kelch-like ECH-associated protein 1 (KEAP1). These results suggest that SV has weight loss and lipid-lowering effects in HFD rats, which may be related to its upregulation of the expressions of -oxidation -elated PPAR-, CPT1, and ACOX1 and the regulation of the expressions of antioxidant pathway-related KEAP1-NRF2-HO-1. Therefore, all these data provide an experimental basis for the development of SV as a functional beverage which is safe, effective, convenient, and inexpensive.
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http://dx.doi.org/10.1155/2020/7083415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487111PMC
September 2020

Improved Phase Stability and Enhanced Luminescence of Calcite Phase LuBO:Ce through Ga Incorporation.

Inorg Chem 2020 Oct 17;59(19):14513-14525. Epub 2020 Sep 17.

School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China.

The application of LuBO:Ce (LBO:Ce) crystal as an excellent scintillation material has been limited due to its poor phase stability at high temperature or high pressure, so improving the phase stability is essential for promoting its development. Ga stabilized LuBO:Ce (LGBO:Ce) is synthesized by solid-state reaction at 1200 °C. Powder X-ray diffraction patterns and Raman spectra at ambient pressure show that all the samples are pure calcite phase. In situ high-pressure synchrotron radiation XRD patterns illustrate that calcite phase LGBO:Ce exhibits more excellent phase stability than that of LBO:Ce under high pressure due to the superior compressibility of the [GaO] octahedral unit. The optical band gap of LGBO decreases from 5.58 to 4.64 eV after introducing 10% Ga, which leads to the decreased nonradiative transition and about double luminescence intensity as expected. More interestingly, the charge transition from O to Ce is observed at about 290 nm in the absorption spectra. The X-ray photoelectron spectroscopy spectra indicate the ratio of Ce/Ce increases with increasing concentration of Ga, which can be attributed to the variation of energy separation between the 4f ground state of Ce and the Fermi energy level position. In contrast to the enhancement of PL intensity, the integrated X-ray excited luminescence intensity decreases after Ga incorporation attributing to the result of both decreased effective atomic number and ionization energy between 5d level and conduction band. The thermal luminescence spectra show that after the incorporation of Ga the oxygen vacancy and intrinsic defects in LBO remain unchanged but that the concentration of oxygen vacancy significantly reduces. The mechanism of Ga incorporation on phase stability and luminescence properties of LBO:Ce has been proposed and discussed systematically.
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http://dx.doi.org/10.1021/acs.inorgchem.0c02299DOI Listing
October 2020

Protective effect and mechanism of mesenchymal stem cells on heat stroke induced intestinal injury.

Exp Ther Med 2020 Oct 27;20(4):3041-3050. Epub 2020 Jul 27.

Department of Critical Care Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, P.R. China.

Heat stroke (HS) is considered to be a severe systemic inflammatory reaction disease that is caused by high fever. The mortality of HS is high worldwide due to the lack of effective treatments. Presently, mesenchymal stem cells (MSCs) have been demonstrated to serve roles in inflammation and immune regulation. Therefore, the current study aimed to investigate the protective effect and mechanism of MSCs against the HS-induced inflammatory response and organ dysfunction. A rat model of HS was induced by a high-temperature environment and treated with MSCs via tail veins. The levels of molecular markers of organ function, inflammatory factors and chemokines were examined at days 1, 7, 14 and 28. Histological staining was performed on the intestines of rats and control groups, and the Chiu's scores of the two groups were compared. The results revealed that MSCs injection significantly reduced the mortality and inhibited the circulatory inflammatory response. Additionally, main organ function, such as in the liver and kidney, were significantly improved following MSCs infusion in HS rats. Furthermore, MSCs treatment significantly improved edema, necrosis and villus exfoliation of intestinal mucosa, and reduced the inflammatory response of intestinal tissue. These results indicated that MSC infusion had therapeutic effects on HS of rats by regulating the circulatory and intestinal inflammatory response. Moreover, MSCs may be able to protect organ function and promote tissue repair in HS. The results of the current study indicated that MSCs may be used as a potential method to treat HS and the resulting organ dysfunction.
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http://dx.doi.org/10.3892/etm.2020.9051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444330PMC
October 2020

Establishment and effectiveness evaluation of a scoring system for exertional heat stroke by retrospective analysis.

Mil Med Res 2020 08 27;7(1):40. Epub 2020 Aug 27.

Department of Critical Care Medicine, The First Medical Centre, Chinese PLA General Hospital, No. 28, Fuxing Road, Haidian District, Beijing, 100853, China.

Background: Heat stroke (HS) is a serious, life-threatening disease. However, there is no scoring system for HS so far. This research is to establish a scoring system that can quantitatively assess the severity of exertional heat stroke (EHS).

Methods: Data were collected from a total of 170 exertional heat stroke (EHS) patients between 2005 and 2016 from 52 hospitals in China. Univariate statistical methods and comparison of the area under the receiver operating characteristic (ROC) curve (AUC) were used to screen exertional heat stroke score (EHSS) parameters, including but not limited body temperature (T), Glasgow Coma Scale (GCS) and others. By comparing the sizes of the AUCs of the APACHE II, SOFA and EHSS assessments, the effectiveness of EHSS in evaluating the prognosis of EHS patients was verified.

Results: Through screening with a series of methods, as described above, the present study determined 12 parameters - body temperature (T), GCS, pH, lactate (Lac), platelet count (PLT), prothrombin time (PT), fibrinogen (Fib), troponin I (TnI), aspartate aminotransferase (AST), total bilirubin (TBIL), creatinine (Cr) and acute gastrointestinal injury (AGI) classification - as EHSS parameters. It is a 0-47 point system designed to reflect increasing severity of heat stroke. Low (EHSS< 20) and high scores (EHSS> 35) showed 100% survival and 100% mortality, respectively. We found that AUCEHSS > AUCSOFA > AUCAPACHE II.

Conclusion: A total of 12 parameters - T, GCS, pH, Lac, PLT, PT, Fib, TnI, AST, TBIL, Cr and gastrointestinal AGI classification - are the EHSS parameters with the best effectiveness in evaluating the prognosis of EHS patients. As EHSS score increases, the mortality rate of EHS patients gradually increases.
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http://dx.doi.org/10.1186/s40779-020-00269-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453553PMC
August 2020

Computational Prediction of Drug Responses in Cancer Cell Lines From Cancer Omics and Detection of Drug Effectiveness Related Methylation Sites.

Front Genet 2020 7;11:917. Epub 2020 Aug 7.

Key Laboratory of Plateau Biological Adaptation and Evolution, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Accurately predicting the response of a cancer patient to a therapeutic agent remains an important challenge in precision medicine. With the rise of data science, researchers have applied computational models to study the drug inhibition effects on cancers based on cancer genomics and transcriptomics. Moreover, a common epigenetic modification, DNA methylation, has been related to the occurrence and development of cancer, as well as drug effectiveness. Therefore, it is helpful for improvement of drug response prediction through exploring the relationship between DNA methylation and drug effectiveness. Here, we proposed a computational model to predict drug responses in cancers through integration of cancer genomics, transcriptomics, epigenomics, and compound chemical properties. Meanwhile, we applied a regularized regression model (Least Absolute Shrinkage and Selection Operator, lasso) to detect the methylation sites that were closely related to drug effectiveness. The prediction models were trained on a well-known pharmacogenomics data resource, Genomics of Drug Sensitivity in Cancer (GDSC). The cross-validation indicates that the performance of the prediction model using DNA methylation is comparable to that of using other cancer omics, including oncogene mutation and gene expression data. It indicates the important role of DNA methylation in prediction of drug responses. Encyclopedia of DNA Elements (ENCODE) and Transcriptional Regulatory Relationships Unraveled by Sentence-based Text mining (TRRUST2) database analyses suggest that the methylation sites associated with drug effectiveness are mainly located in the transcription factor (TF) binding region. Therefore, we hypothesized that the sensitivity of cancer cells to drugs could be regulated by changing the methylation modification of TF binding region. In conclusion, we confirmed the important role of DNA methylation in prediction of drug responses, and provided some methylation sites that closely related to the drug effectiveness, which may be a great regulatory target for improvement of drug treatment effects on cancer patients.
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http://dx.doi.org/10.3389/fgene.2020.00917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426400PMC
August 2020

Aminoguanidine inhibits IL-1β-induced protein expression of iNOS and COX-2 by blocking the NF-κB signaling pathway in rat articular chondrocytes.

Exp Ther Med 2020 Sep 17;20(3):2623-2630. Epub 2020 Jul 17.

Department of Clinical Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150000, P.R. China.

Osteoarthritis is a chronic joint disease which has a serious impact on the health and quality of life of affected humans and animals. As an inhibitor of inducible nitric oxide synthase (iNOS), aminoguanidine (AG) displays anti-inflammatory effects. The purpose of the present study was to investigate the effect of AG on the expression of iNOS and cyclooxygenase-2 (COX-2), and the activity of the NF-κB signaling pathway in rat chondrocytes stimulated by interleukin-1β (IL-1β). The viability of chondrocytes treated with AG (0.3, 1 or 3 mM) alone was determined using a Cell Counting Kit-8 assay. Subsequently, the chondrocytes were treated with either 10 ng/ml IL-1β alone, or co-treated with increasing concentrations of AG (0.3, 1 or 3 mM) and 10 ng/ml IL-1β. The protein levels of COX-2, iNOS, phosphorylated (p)-p65, p65, p-NF-κβ inhibitor α (IκBα), IκBα, p-inhibitor of NF-κβ-β (IKKβ) and IKKβ were evaluated by western blotting. NF-κB translocation was determined by immunofluorescence analysis. Western blotting and reverse transcription-quantitative PCR were used to detect expression levels of relevant proteins/genes. The results suggested that the inhibitory effect of AG on the protein and gene expression levels of iNOS and COX-2 in IL-1β-treated chondrocytes was dose-dependent. In addition, AG decreased the level of phosphorylation of IKKβ, IκBα and NF-κB p65, the degradation of IKKβ, IκBα and p65, and the translocation of NF-κB in IL-1β-stimulated chondrocytes. The most significant inhibitory effect of AG was observed at a concentration of 1 mM. Therefore, the present study suggested that AG may serve as a potential agent to reduce the inflammatory response of chondrocytes stimulated by IL-1β.
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http://dx.doi.org/10.3892/etm.2020.9021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401635PMC
September 2020

Safety considerations for nanoparticle gene delivery in pediatric brain tumors.

Nanomedicine (Lond) 2020 08 23;15(18):1805-1815. Epub 2020 Jul 23.

Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Current standard of care for many CNS tumors involves surgical resection followed by chemotherapy and/or radiation. Some pediatric brain tumor types are infiltrative and diffuse in nature, which reduces the role for surgery. Furthermore, children are extremely vulnerable to neurological sequelae from surgery and radiation therapy, thus alternative approaches are in critical need. As molecular targets underlying various cancers become more clearly defined, there is an increasing push for targeted gene therapies. Viral vectors and nonviral nanoparticles have been thoroughly investigated for gene delivery and show promise as vectors for gene therapy for pediatric brain cancer. Here, we review inorganic and organic materials in development for nanoparticle gene delivery to the brain with a particular focus on safety.
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http://dx.doi.org/10.2217/nnm-2020-0110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441302PMC
August 2020

Biomass pyrolysis with alkaline-earth-metal additive for co-production of bio-oil and biochar-based soil amendment.

Sci Total Environ 2020 Nov 6;743:140760. Epub 2020 Jul 6.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, 219 Ningliu Road, Nanjing 210044, China.

The alkaline-earth-metal (AEM) has a good performance on modification of both bio-oil and biochar during biomass pyrolysis. In this work, the pyrolysis of rice husk (RH) in the presence of CaO, CaCO, MgO and MgCO was comparatively studied for selecting an appropriate AEM additive to balance the qualities of pyrolytic products. Pyrolysis of RH with the AEM additives could decrease the acids content and increase the hydrocarbons content in bio-oil. Compared with the Ca-additives (i.e., CaO, CaCO), the Mg-additives (i.e., MgO, MgCO) were more beneficial for enhancing the hydrocarbons production. The addition of biochar to soil can significantly enhance the water retention. RHC-MgCO had a maximum water retention capacity, while RHC-MgO had a minimum water retention capacity due to its lowest specific surface area. Additionally, the Mg-modified biochar had a much higher nutrient (i.e., K, PO) adsorption capacity. In particular, RHC-MgO with a lowest specific surface area had a highest PO adsorption capacity, which was evidenced by the adsorption of PO onto biochar mainly controlled by the chemisorption process. PO adsorbed in the RHC-MgO released rapidly indicating its low PO retention capacity. In general, MgCO would be an appropriate candidate that is used in pyrolysis of biomass for co-production of bio-oil and biochar composite with high capacities of water/nutrient adsorption and retention for soil amendment.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140760DOI Listing
November 2020

Surface Engineering of Liquid Metal Nanodroplets by Attachable Diblock Copolymers.

ACS Nano 2020 Aug 10;14(8):9884-9893. Epub 2020 Jul 10.

Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

Liquid metal (LM) micro/nano droplets have promising applications in various fields such as flexible electronics, catalysis, and soft composites as well as biomedicines. However, the preparation of highly stable LM nanodroplets suspension based on eutectic gallium/indium (EGaIn) alloys is still challenging. Herein, we report a general and robust strategy to fabricate EGaIn nanodroplets stabilized by polymer brushes (polymer brushes/EGaIn nanodroplets) attachment of well-defined diblock copolymers with short poly(acrylic acid) (PAA) anchoring segments. Under ultrasonication, the anchoring PAA block is attached onto the gallium oxide "skin" layer of EGaIn nanodroplets to form polymer brushes. The attachable diblock copolymer surfactants allow for highly efficient formation of EGaIn nanodroplets in high yield and with narrow size distribution. The polymer brushes/EGaIn nanodroplets contain very low fractions of attached polymer (<1 wt %) and exhibit high colloidal stability (>30 days) and good redispersibility. Precise control of polymer architecture by atom-transfer radical polymerization was employed to prepare various block copolymers for suspensions in a variety of solvents.
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http://dx.doi.org/10.1021/acsnano.0c02720DOI Listing
August 2020

L-Theanine Reduced the Development of Knee Osteoarthritis in Rats via Its Anti-Inflammation and Anti-Matrix Degradation Actions: In Vivo and In Vitro Study.

Nutrients 2020 Jul 3;12(7). Epub 2020 Jul 3.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

The etiology of osteoarthritis (OA) is multifactorial, with no effective disease-modifying-drugs. L-theanine has been reported to inhibit inflammatory responses in some diseases and this study aimed to investigate the effect of L-theanine on Interleukin-1(IL-1)β-stimulated chondrocytes, and in an injury-induced OA rat model. Primary chondrocytes were stimulated by IL-1β (10 ng/mL) for 24 h and then co-cultured with L-theanine for 24 h. The effects of L-theanine on IL-1β-stimulated expression of pro-inflammatory cytokines and hydrolytic enzyme were analyzed using Western blotting, quantitative polymerase chain reaction (q-PCR) and enzyme-linked immunosorbent assay (ELISA) kits. An immunofluorescence assay was used to detect nuclear factor kappa B (NF-κB) phosphorylation. OA was induced by anterior cruciate ligament transection (ACLT) surgery in rats and celecoxib was used as a positive control. OA severity was measured using the Osteoarthritis Research Society International (OARSI) grading system to describe histological changes. The results showed that L-theanine decreased the expression of pro-inflammatory mediators, including cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE-2), inducible nitric oxide synthase (iNOS), and nitric oxide (NO), both in vivo and in vitro. L-theanine treatment inhibited IL-1β-induced upregulation of matrix metalloproteinases (MMP)-3 and MMP-13, as well as inhibited NF-κB p65 activation. In vivo animal model showed that L-theanine administration (200 mg/kg) significantly alleviated OA lesions and decreased OARSI score. Our data indicated that L-theanine decreased inflammatory cytokines and protected extracellular matrix degradation through inhibition of the NF-κB pathway, and L-theanine may be considered a promising therapeutic strategy in OA prevention.
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http://dx.doi.org/10.3390/nu12071988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400703PMC
July 2020

The effect of antibiotic resistance on Helicobacter pylori eradication efficacy: A systematic review and meta-analysis.

Helicobacter 2020 Aug 12;25(4):e12714. Epub 2020 Jun 12.

Department of Gastroenterology, First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: The occurrence of antibiotic-resistant strains has been rapidly increasing due to the wide use of antibiotics. To evaluate the current effects of antibiotic resistance on Helicobacter pylori eradication efficacy, we conducted this systematic review and meta-analysis.

Methods: Literature searches were conducted in the following databases: PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials. Statistical analysis was performed using STATA version 12.0 (StataCorp LP, College Station, TX, USA).

Results: A total of 120 studies, including 28 707 patients, were assessed. Only first-line therapy was considered. The pooled RR of eradication rate in patients with Helicobacter pylori strains sensitive versus resistant to clarithromycin was 0.682 (95% CI: 0.636-0.731). The pooled RR of eradication rate in patients with Helicobacter pylori strains sensitive versus resistant to metronidazole was 0.843 (95% CI: 0.810-0.877). The pooled RR of eradication rate in patients with Helicobacter pylori strains sensitive versus resistant to levofloxacin was 0.794 (95% CI: 0.669-0.941). The pooled RR of eradication rate in patients with Helicobacter pylori strains sensitive versus resistant to dual clarithromycin and metronidazole was 0.674 (95% CI: 0.590-0.770).

Conclusion: Antibiotic resistance causes a decrease in the eradication rate of H pylori today. Quadruple concomitant therapy may overcome the declining H pylori eradication rate caused by metronidazole-only resistance.
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http://dx.doi.org/10.1111/hel.12714DOI Listing
August 2020

LncRNA MINCR promotes the development of liver cancer by regulating microRNA-107/β-catenin.

J BUON 2020 Mar-Apr;25(2):972-980

Department of Liver and Infectious Diseases, Liver Disease Research Center, the second People's Hospital of Yunnan Province, Kunming 650021, China.

Purpose: To investigate the level of long noncoding ribonucleic acid (lncRNA) MINCR in hepatocellular carcinoma (HCC), and to further investigate whether it can promote the development of HCC through modulating microRNA-107/β-catenin.

Methods: MINCR level in 52 pairs of HCC tumor tissues as well as adjacent tissues and HCC cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). MINCR knockdown model was constructed using lentivirus in the carcinoma cell lines. Furthermore, cell counting kit-8 (CCK-8), plate cloning and apoptosis assay were used to analyze the effect of MINCR on the biological function of HCC cells. Finally, cell recovery experiment was performed to explore its potential mechanism and the association between MINCR and microRNA-107/β-catenin.

Results: qPCR results showed that the level of MINCR in HCC was remarkably higher than that in adjacent tissues, and the difference was statistically significant. Compared with patients with low level of MINCR, patients with high level of MINCR had lower overall survival rate. Similarly, the cell proliferation ability of sh-MINCR group was remarkably decreased while the apoptosis ability was oppositely increased when compared with the short hairpin RNA (shRNA) group. In addition, studies have demonstrated that the levels of microRNA-107 and MINCR in HCC tissues were negatively related. In our study, dual-luciferase reporting assay verified that MINCR can be targeted by microRNA-107 through certain binding site. In addition, MINCR was confirmed to be able to further regulate the malignant progression of HCC through microRNA-107/β-catenin.

Conclusions: The level of MINCR was remarkably increased in HCC, which was associated with poor prognosis of HCC patients. Moreover, MINCR was capable of promoting the proliferation and inhibiting apoptosis of liver cancer cells via regulating microRNA-107/β-catenin.
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February 2021
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