Publications by authors named "Yuan Quan"

600 Publications

Revealing Ionic Signal Enhancement with Probe Grafting Density on the Outer Surface of Nanochannels.

Anal Chem 2021 Sep 14. Epub 2021 Sep 14.

State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, P. R. China.

Probe-modified nanopores/nanochannels are one of the most advanced sensors because the probes interact strongly with ions and targets in nanoconfinement and create a sensitive and selective ionic signal. Recently, ionic signals have been demonstrated to be sensitive to the probe-target interaction on the outer surface of nanopores/nanochannels, which can offer more open space for target recognition and signal conversion than nanoconfined cavities. To enhance the ionic signal, we investigated the effect of grafting density, a critical parameter of the sensing interface, of the probe on the outer surface of nanochannels on the change rate of the ionic signal before and after target recognition (β). Electroneutral peptide nucleic acids and negatively charged DNA are selected as probes and targets, respectively. The experimental results showed that when adding the same number of targets, the β value increased with the probe grafting density on the outer surface. A theoretical model with clearly defined physical properties of each probe and target has been established. Numerical simulations suggest that the decrease of the background current and the aggregation of targets at the mouth of nanochannels with increasing probe grafting density contribute to this enhancement. This work reveals the signal mechanism of probe-target recognition on the outer surface of nanochannels and suggests a general approach to the nanochannel/nanopore design leading to sensitivity improvement on the basis of relatively good selectivity.
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http://dx.doi.org/10.1021/acs.analchem.1c03010DOI Listing
September 2021

Persisting lung pathogenesis and minimum residual virus in hamster after acute COVID-19.

Protein Cell 2021 Sep 7. Epub 2021 Sep 7.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361000, China.

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http://dx.doi.org/10.1007/s13238-021-00874-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422370PMC
September 2021

CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1.

Cell Death Dis 2021 Aug 30;12(9):820. Epub 2021 Aug 30.

National Engineering Research Center for Nanomedicine, Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Tongji Hospital, Huazhong University of Science and Technology, 430074, Wuhan, China.

Failures to treat triple-negative breast cancer (TNBC) are mainly due to chemoresistance or radioresistance. We and others previously discovered that zinc finger E-box-binding homeobox 1 (ZEB1) is a massive driver causing these resistance. However, how to dynamically modulate the intrinsic expression of ZEB1 during cell cycle progression is elusive. Here integrated affinity purification combined with mass spectrometry and TCGA analysis identify a cell cycle-related E3 ubiquitin ligase, checkpoint with forkhead and ring finger domains (CHFR), as a key negative regulator of ZEB1 in TNBC. Functional studies reveal that CHFR associates with and decreases ZEB1 expression in a ubiquitinating-dependent manner and that CHFR represses fatty acid synthase (FASN) expression through ZEB1, leading to significant cell death of TNBC under chemotherapy. Intriguingly, a small-molecule inhibitor of HDAC under clinical trial, Trichostatin A (TSA), increases the expression of CHFR independent of histone acetylation, thereby destabilizes ZEB1 and sensitizes the resistant TNBC cells to conventional chemotherapy. In patients with basal-like breast cancers, CHFR levels significantly correlates with survival. These findings suggest the therapeutic potential for targeting CHFR-ZEB1 signaling in resistant malignant breast cancers.
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http://dx.doi.org/10.1038/s41419-021-04114-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405615PMC
August 2021

Efficient intracellular delivery of proteins by a multifunctional chimaeric peptide in vitro and in vivo.

Nat Commun 2021 08 26;12(1):5131. Epub 2021 Aug 26.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Centers of Biological Products, School of Public Health, Xiamen University, Xiamen, China.

Protein delivery with cell-penetrating peptide is opening up the possibility of using targets inside cells for therapeutic or biological applications; however, cell-penetrating peptide-mediated protein delivery commonly suffers from ineffective endosomal escape and low tolerance in serum, thereby limiting in vivo efficacy. Here, we present an intracellular protein delivery system consisting of four modules in series: cell-penetrating peptide, pH-dependent membrane active peptide, endosome-specific protease sites and a leucine zipper. This system exhibits enhanced delivery efficiency and serum tolerance, depending on proteolytic cleavage-facilitated endosomal escape and leucine zipper-based dimerisation. Intravenous injection of protein phosphatase 1B fused with this system successfully suppresses the tumour necrosis factor-α-induced systemic inflammatory response and acetaminophen-induced acute liver failure in a mouse model. We believe that the strategy of using multifunctional chimaeric peptides is valuable for the development of cell-penetrating peptide-based protein delivery systems, and facilitate the development of biological macromolecular drugs for use against intracellular targets.
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http://dx.doi.org/10.1038/s41467-021-25448-zDOI Listing
August 2021

A cast-free approach to fabricating an implant-supported interim restoration: A dental technique.

J Prosthet Dent 2021 Aug 17. Epub 2021 Aug 17.

Professor, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China. Electronic address:

A cast-free approach is described to fabricate an implant-supported interim restoration by using the postoperative cone beam computed tomography (CBCT) scan to locate the placed implant. A postoperative intraoral scan was aligned to the postoperative CBCT scan through a dental implant planning software program. An attached interim abutment and implant analog complex was then scanned and superimposed on the placed implant in the postoperative CBCT scan. Once the best alignment was achieved, a virtual cast was generated, and an interim restoration with bilateral positioning wings was fabricated on the interim abutment and inserted during the second-stage surgery. This technique offers a cast-free approach to inserting an implant-supported interim restoration immediately after the second-stage surgery to guide the healing of the soft tissue that can minimize chairside time and optimize the clinical workflow.
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http://dx.doi.org/10.1016/j.prosdent.2021.07.013DOI Listing
August 2021

Edge-Preserving Median Filter and Weighted Coding with Sparse Nonlocal Regularization for Low-Dose CT Image Denoising Algorithm.

J Healthc Eng 2021 26;2021:6095676. Epub 2021 Jul 26.

School of Mathematics and Computing Science, Guilin University of Electronic Technology, Guilin 541004, Guangxi, China.

The impulse noise in CT image was removed based on edge-preserving median filter algorithm. The sparse nonlocal regularization algorithm weighted coding was used to remove the impulse noise and Gaussian noise in the mixed noise, and the peak signal-to-noise ratio (PSNR) and structural similarity index (SSIM) were calculated to evaluate the quality of the denoised CT image. It was found that in nine different proportions of Gaussian noise and salt-and-pepper noise in Shepp-Logan image and CT image processing, the PSNR and SSIM values of the proposed denoising algorithm based on edge-preserving median filter (EP median filter) and weighted encoding with sparse nonlocal regularization (WESNR) were significantly higher than those of using EP median filter and WESNR alone. It was shown that the weighted coding algorithm based on edge-preserving median filtering and sparse nonlocal regularization had potential application value in low-dose CT image denoising.
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http://dx.doi.org/10.1155/2021/6095676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8331292PMC
July 2021

Turnip mosaic virus impairs perinuclear chloroplast clustering to facilitate viral infection.

Plant Cell Environ 2021 Jul 30. Epub 2021 Jul 30.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China.

Chloroplasts play crucial roles in plant defence against viral infection. We now report that chloroplast NADH dehydrogenase-like (NDH) complex M subunit gene (NdhM) was first up-regulated and then down-regulated in turnip mosaic virus (TuMV)-infected N. benthamiana. NbNdhM-silenced plants were more susceptible to TuMV, whereas overexpression of NbNdhM inhibited TuMV accumulation. Overexpression of NbNdhM significantly induced the clustering of chloroplasts around the nuclei and disturbing this clustering facilitated TuMV infection, suggesting that the clustering mediated by NbNdhM is a defence against TuMV. It was then shown that NbNdhM interacted with TuMV VPg, and that the NdhMs of different plant species interacted with the proteins of different viruses, implying that NdhM may be a common target of viruses. In the presence of TuMV VPg, NbNdhM, which is normally localized in the nucleus, chloroplasts, cell periphery and chloroplast stromules, colocalized with VPg at the nucleus and nucleolus, with significantly increased nuclear accumulation, while NbNdhM-mediated chloroplast clustering was significantly impaired. This study therefore indicates that NbNdhM has a defensive role in TuMV infection probably by inducing the perinuclear clustering of chloroplasts, and that the localization of NbNdhM is altered by its interaction with TuMV VPg in a way that promotes virus infection.
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http://dx.doi.org/10.1111/pce.14157DOI Listing
July 2021

Lactulose Improves Neurological Outcomes by Repressing Harmful Bacteria and Regulating Inflammatory Reactions in Mice After Stroke.

Front Cell Infect Microbiol 2021 13;11:644448. Epub 2021 Jul 13.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neurotrauma, Neurorepair, and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin, China.

Background And Objective: Gut microbiota dysbiosis following stroke affects the recovery of neurological function. Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function. We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.

Methods: Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose. After 14 consecutive days of lactulose administration, feces, plasma, and organs were collected. 16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.

Results: Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut. In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.

Conclusion: Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.
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http://dx.doi.org/10.3389/fcimb.2021.644448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313872PMC
August 2021

Systems Pharmacology-Based Precision Therapy and Drug Combination Discovery for Breast Cancer.

Cancers (Basel) 2021 Jul 17;13(14). Epub 2021 Jul 17.

Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.

Breast cancer (BC) is a common disease and one of the main causes of death in females worldwide. In the omics era, researchers have used various high-throughput sequencing technologies to accumulate massive amounts of biomedical data and reveal an increasing number of disease-related mutations/genes. It is a major challenge to use these data effectively to find drugs that may protect human health. In this study, we combined the GeneRank algorithm and gene dependency network to propose a precision drug discovery strategy that can recommend drugs for individuals and screen existing drugs that could be used to treat different BC subtypes. We used this strategy to screen four BC subtype-specific drug combinations and verified the potential activity of combining gefitinib and irinotecan in triple-negative breast cancer (TNBC) through in vivo and in vitro experiments. The results of cell and animal experiments demonstrated that the combination of gefitinib and irinotecan can significantly inhibit the growth of TNBC tumour cells. The results also demonstrated that this systems pharmacology-based precision drug discovery strategy effectively identified important disease-related genes in individuals and special groups, which supports its efficiency, high reliability, and practical application value in drug discovery.
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http://dx.doi.org/10.3390/cancers13143586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305788PMC
July 2021

A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters.

Sci Transl Med 2021 08 20;13(606). Epub 2021 Jul 20.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences & School of Public Health, Xiamen University, Xiamen 361102, P. R. China.

Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate-modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both T1- and T2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by the absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit-based SARS-CoV-2 vaccine candidate.
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http://dx.doi.org/10.1126/scitranslmed.abg1143DOI Listing
August 2021

PODN is a prognostic biomarker and correlated with immune infiltrates in osteosarcoma.

Cancer Cell Int 2021 Jul 17;21(1):381. Epub 2021 Jul 17.

Department of Orthopedics, Children's Hospital of Soochow University, Su Zhou, 215025, Jiang Su, China.

Background: Osteosarcoma was the most common primary bone malignancy in children and adolescents. It was imperative to identify effective prognostic biomarkers for this cancer. This study was aimed to identify potential crucial genes of osteosarcoma by integrated bioinformatics analysis.

Methods: Identification of differentially expressed genes from public data gene expression profiles (GSE42352), functional and pathway enrichment analysis, protein-protein interaction (PPI) network construction and module analysis, Cox regression and survival analysis was conducted.

Results: Totally 17 co-differential genes were found to be differentially expressed. These genes were enriched in biological processes, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) pathway of inflammatory immune response. PPI network was constructed with 63 differentially expressed genes that co-existed between the test set and the validation set. The area under the receiver operating characteristic curve (AUC value) was 0.855, which indicated that the expression of PODN had a good diagnostic value for osteosarcoma. Furthermore, Cox regression and survival analysis revealed 5 genes were statistically significant.

Conclusions: PODN was regarded as a potential biomarker for the diagnosis and prognosis of osteosarcoma, ACTA2, COL6A1, FAP, OLFML2B and COL6A3, can be used as potential prognostic indicators for osteosarcoma.
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http://dx.doi.org/10.1186/s12935-021-02086-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285818PMC
July 2021

Management of systemic risk factors ahead of dental implant therapy: A beard well lathered is half shaved.

J Leukoc Biol 2021 09 7;110(3):591-604. Epub 2021 Jul 7.

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

As the most successful therapy for missing teeth, dental implant has become increasingly prevalent around the world. A lot of papers have reported diverse local risk factors affecting the success and survival rate of dental implants, either for a short or a long period. However, there are also many types of systemic disorders or relatively administrated medicine that may jeopardize the security and success of dental implant treatment. Additionally, the coronavirus disease 2019 pandemic also poses a challenge to dental implant clinicians. Some of these risk factors are clinically common but to some extent unfamiliar to dentists, thus optimal measurements are often lacking when they occur in dental clinics. In this review, we analyze potential systemic risk factors that may affect the success rate of dental implants. Some of them may affect bone mineral density or enhance the likelihood of local infection, thus impeding osseointegration. Others may even systemically increase the risk of the surgery and threaten patients' life. In order to help novices receive high-risk patients who need to get dental implant treatment in a more reasonable way, we accordingly review recent research results and clinical experiments to discuss promising precautions, such as stopping drugs that impact bone mineral density or the operation, and addressing any perturbations on vital signs.
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http://dx.doi.org/10.1002/JLB.6MR0621-760RRDOI Listing
September 2021

Dynamics Analysis of Firing Patterns in Pre-Bötzinger Complex Neurons Model.

Front Comput Neurosci 2021 15;15:591037. Epub 2021 Jun 15.

School of Mathematics and Information Science, Guangxi University, Guangxi, China.

Pre-Bötzinger complex (PBC) neurons located in mammalian brain are the necessary conditions to produce respiratory rhythm, which has been widely verified experimentally and numerically. At present, one of the two different types of bursting mechanisms found in PBC mainly depends on the calcium-activated of non-specific cation current ( ). In order to study the influence of and stimulus current in PBC inspiratory neurons, a single compartment model was simplified, and firing patterns of the model was discussed by using stability theory, bifurcation analysis, fast, and slow decomposition technology combined with numerical simulation. Under the stimulation of different somatic applied currents, the firing behavior of neurons are studied and exhibit multiple mix bursting patterns, which is helpful to further understand the mechanism of respiratory rhythms of PBC neurons.
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http://dx.doi.org/10.3389/fncom.2021.591037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239244PMC
June 2021

Phyllotaxis development: a lesson from the Asteraceae family.

Trends Plant Sci 2021 Sep 23;26(9):873-875. Epub 2021 Jun 23.

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China. Electronic address:

Phyllotaxis refers to the spatial arrangement of leaves and flowers on a stem. A recent study by Zhang et al. described the developmental process underlying phyllotaxis establishment in the capitulum of Gerbera hybrida. This work represents a cornerstone for studying the development and diversification mechanisms of capitula in the Asteraceae.
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http://dx.doi.org/10.1016/j.tplants.2021.06.002DOI Listing
September 2021

A Novel Technique to Align the Intraoral Scans to the Virtual Articulator and Set the Patient-Specific Sagittal Condylar Inclination.

J Prosthodont 2021 Jun 25. Epub 2021 Jun 25.

Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.

Customized cast orientations and parameter settings of the virtual articulator according to the patient's condyles are indispensable parts of today's digital workflows in prosthodontics. This article describes a digital technique to align the intraoral scans to a virtual articulator by using a facial scanner to locate the patient's cutaneous landmarks of the arbitrary hinge axis and the reference plane, and to customize the sagittal condylar inclination of the virtual articulator through a digital protrusive interocclusal record and a dental computer-aided design software program. It enables individual cast orientations and virtual articulator parameter settings without conventional facebow transferring and bite registration procedures and can be easily integrated with most virtual articulator systems on the market to allow clinicians and technicians to work in a complete digital workflow and facilitate customized treatment planning and dental prosthesis fabrication.
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http://dx.doi.org/10.1111/jopr.13403DOI Listing
June 2021

Impact of TDG supersaturation on native fish species under different hydropower flood discharge programs.

Aquat Toxicol 2021 Aug 10;237:105898. Epub 2021 Jun 10.

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.

Total dissolved gas (TDG) supersaturation caused by the operation of hydropower stations can threaten the survival and swimming performance of fish species. Different modes of hydropower flood discharges (regular vs. intermittent) from the Dagangshan hydropower station in China were studied in July and September 2017 to study the impact of TDG supersaturation on two native fish species in the downstream Dadu River. The average TDG supersaturation value was 114.3% in July under the regular discharge mode. In September, the supersaturation of TDG fluctuated in response to the intermittent discharge mode with an average TDG of 119.3%. Apparent gas bubble trauma was found on young-of-the-year (YOY) Prenant's schizothoracin and elongate loach in cages at different water depths during the flood discharge period. The mortality rate of YOY Prenant's schizothoracin and elongate loach in cages with water depths of 0-1 m were 16.25% and 2.5%, respectively, in July. The fluctuating TDG levels with higher peaks in September caused higher fish mortality rates. The final mortality rates of YOY Prenant's schizothoracin and elongate loach in cages with water depths of 0-1 m were 75% and 33.75%, respectively. Fish in the cages at a 0-3 m water depth survived better than those in the cages at a 0-1 m water depth. The critical swimming speeds (U) of YOY Prenant's schizothoracin and elongate loach without exposure to TDG supersaturation were 11.64 and 16.76 BL s, respectively. U decreased significantly after experiencing the flood discharge period and recovered to the normal level after experiencing the corresponding interval period.
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http://dx.doi.org/10.1016/j.aquatox.2021.105898DOI Listing
August 2021

Aerobic granular sludge treating low-strength municipal wastewater: Efficient carbon, nitrogen and phosphorus removal with hydrolysis-acidification pretreatment.

Sci Total Environ 2021 Oct 5;792:148297. Epub 2021 Jun 5.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China.

Low organic load while high fraction of particulates still challenging the application of aerobic granular sludge process in low-strength municipal wastewater treatment. The feasibility of adopting short cycle length to increase organic load and hydrolysis-acidification pretreatment to enhance anaerobic COD uptake was evaluated. As the cycle length decreased from 4 h to 2 h, the organic loading rate increased from 0.98 to 1.3 g L d and granulation appeared after two weeks. Moreover, with the hydrolysis-acidification pretreatment, the average effluent TN and TP concentrations decreased respectively from 17.8 to 13.7 mg L and 0.76 to 0.41 mg L, meeting the Grade IA of the effluent standards in China. Furthermore, cycle tests were conducted to reveal the underlying mechanism of the pretreatment effects. The results showed that the hydrolysis-acidification pretreatment enhanced the COD storage and phosphorus release in anaerobic phase, and improved the simultaneous nitrification-denitrification process, as well as the phosphorus uptake in aeration phase.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148297DOI Listing
October 2021

Metoprolol attenuates intracerebral hemorrhage-induced cardiac damage by suppression of sympathetic overactivity in mice.

Auton Neurosci 2021 Sep 5;234:102832. Epub 2021 Jun 5.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neurotrauma, Neurorepair, and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin 300052, China. Electronic address:

The high rates of mortality and disability resulting from intracerebral hemorrhage (ICH) are closely related to subsequent cardiac complications. The mechanisms underlying ICH-induced cardiac dysfunction are not fully understood. In this study, we investigated the role of sympathetic overactivity in mediating cardiac dysfunction post ICH in mice. Collagenase-injection ICH model was established in adult male C57BL/6J mice. Neurological function was subsequently evaluated at multiple time points after ICH and cardiac function was measured by echocardiography on 3 and 14 days after ICH. Plasma adrenaline, noradrenaline, cortisol and heart β1 adrenergic receptor (β1-AR) levels were assessed to evaluate sympathetic activity. Picro Sirius Red (PSR) staining was performed to evaluate cardiomyocyte hypertrophy and interstitial fibrosis. Monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin-6(IL-6), nuclear factor kappa-B(NF-κB), NADPH oxidase-2 (NOX2), matrix metalloprotein (MMP-9) and transforming growth factor-beta (TGF-β) levels were assessed to evaluate inflammation, fibrosis and oxidative stress levels in heart after ICH. Macrophages and neutrophils were assessed to evaluate inflammatory cell infiltration in heart after ICH. ICH induced sympathetic excitability, as identified by increased circulating adrenaline, noradrenaline, cortisol levels and β1-AR expression in heart tissue. Metoprolol-treated ICH mice had improved cardiac and neurological function. The suppression of sympathetic overactivity by metoprolol attenuates cardiac inflammation, fibrosis and oxidative stress after ICH. In conclusion, ICH-induced secondary sympathetic overactivity which mediated inflammatory response may play an important role in post-ICH cardiac dysfunction.
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http://dx.doi.org/10.1016/j.autneu.2021.102832DOI Listing
September 2021

Managing Defects Density and Interfacial Strain via Underlayer Engineering for Inverted CsPbI Br Perovskite Solar Cells with All-Layer Dopant-Free.

Small 2021 Jul 12;17(28):e2101902. Epub 2021 Jun 12.

Soochow Institute for Energy and Materials Innovation (SIEMIS), School of Energy, Soochow University, Suzhou, 215006, China.

Inorganic perovskite CsPbI Br has advantages of excellent thermal stability and reasonable bandgap, which make it suitable for top layer of tandem solar cells. Nevertheless, solution-processed all-inorganic perovskites generally suffer from high-density defects as well as significant tensile strain near underlayer/perovskite interface, both leading to compromised device efficiency and stability. In this work, the defect density as well as interfacial tensile strain in inverted CsPbI Br perovskite solar cells (PeSCs) is remarkably reduced by using a bilayer underlayer composed of dopant-free 2,2',7,7'-tetrakis(N,N-dip-methoxyphenylamine)-9,9'-spirobifluorene (Spiro-OMeTAD) and copper phthalocyanine 3,4',4″,4'″-tetrasulfonated acid tetrasodium salt (TS-CuPc) nanoparticles. As compared to control devices with pristine Spiro-OMeTAD, devices based on Spiro-OMeTAD/TS-CuPc exhibit remarkably improved photovoltaic performance and enhanced thermal/humidity stability due to the better perovskite crystallization, improved interfacial passivation, and hole-collection as well as efficient interfacial strain release. As a result, a champion efficiency of 14.85% can be achieved, which is approaching to the best reported for dopant-free and inverted all-inorganic PeSCs. The work thus provides an efficient strategy to simultaneously regulate the defects density and strain issue related to inorganic perovskites.
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http://dx.doi.org/10.1002/smll.202101902DOI Listing
July 2021

Design and Engineering of Hypoxia and Acidic pH Dual-Stimuli-Responsive Intelligent Fluorescent Nanoprobe for Precise Tumor Imaging.

Small 2021 07 12;17(28):e2100243. Epub 2021 Jun 12.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Chemical Biology and Nanomedicine (ICBN), Hunan University, Changsha, 410082, China.

Stimulus-responsive fluorescence imaging modality shows great promise for detection of tumor due to the advantages of high sensitivity, simplicity and noninvasiveness. However, some non-cancer regions including nodules and inflammation may also exhibit a stimulus-related characteristic, which cause the problem of nonspecific responsiveness and then cause "false positive" results for tumor recognition. Herein, hypoxia and acidic pH, two typical features strongly associated with tumor invasion, progression and metastasis in tumor microenvironment (TME), are chosen as dual stimuli to fabricate "dual lock-and-key" fluorescent nanoprobe for highly specific and precise imaging of tumor cells. Mesoporous silica coated gold nanorods ([email protected] ) are employed as nanocarrier and nanoquencher to load the pH-sensitive fluorescent reporter (Rho-TP). Azobenzene (azo) which can be reduced to amines by the highly expressed azoreductase under hypoxic conditions, is elected as the effective gatekeeper for [email protected] by forming complex with β-cyclodextrin polymer via host-guest interaction (azo/β-CDP). By elaborately combining the hypoxia-responsive gatekeeper and pH-responsive fluorescent signal reporter into one nanoprobe, sensitive and specific imaging of tumor cells can be realized. The fabricated dual lock-and-key fluorescent nanoprobe successfully further apply in tumor-bearing mice model, which indicate potential of early diagnosis and assessment of cancer treatment.
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http://dx.doi.org/10.1002/smll.202100243DOI Listing
July 2021

Probiotics ameliorate alveolar bone loss by regulating gut microbiota.

Cell Prolif 2021 Jul 7;54(7):e13075. Epub 2021 Jun 7.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Objectives: Oestrogen deficiency is an aetiological factor of postmenopausal osteoporosis (PMO), which not only decreases bone density in vertebrae and long bone but also aggravates inflammatory alveolar bone loss. Recent evidence has suggested the critical role of gut microbiota in osteoimmunology and its influence on bone metabolisms. The present study aimed to evaluate the therapeutic effects of probiotics on alveolar bone loss under oestrogen-deficient condition.

Materials And Methods: Inflammatory alveolar bone loss was established in ovariectomized (OVX) rats, and rats were daily intragastrically administered with probiotics until sacrifice. Gut microbiota composition, intestinal permeability, systemic immune status and alveolar bone loss were assessed to reveal the underlying correlation between gut microbiota and bone metabolisms.

Results: We found administration of probiotics significantly prevented inflammatory alveolar bone resorption in OVX rats. By enriching butyrate-producing genera and enhancing gut butyrate production, probiotics improved intestinal barrier and decreased gut permeability in the OVX rats. Furthermore, the oestrogen deprivation-induced inflammatory responses were suppressed in probiotics-treated OVX rats, as reflected by reduced serum levels of inflammatory cytokines and a balanced distribution of CD4 IL-17A Th17 cells and CD4 CD25 Foxp3 Treg cells in the bone marrow.

Conclusions: This study demonstrated that probiotics can effectively attenuate alveolar bone loss by modulating gut microbiota and further regulating osteoimmune response and thus represent a promising adjuvant in the treatment of alveolar bone loss under oestrogen deficiency.
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http://dx.doi.org/10.1111/cpr.13075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249787PMC
July 2021

Alcohol-impaired Walking in 16 Countries: A Theory-Based Investigation.

Accid Anal Prev 2021 Sep 4;159:106212. Epub 2021 Jun 4.

Chang'an University, China.

Alcohol is a global risk factor for road trauma. Although drink driving has received most of the scholarly attention, there is growing evidence of the risks of alcohol-impaired walking. Alcohol-impaired pedestrians are over-represented in fatal crashes compared to non-impaired pedestrians. Additionally, empirical evidence shows that alcohol intoxication impairs road-crossing judgements. Besides some limited early research, much is unknown about the global prevalence and determinants of alcohol-impaired walking. Understanding alcohol-impaired walking will support health promotion initiatives and injury prevention. The present investigation has three aims: (1) compare the prevalence of alcohol-impaired walking across countries; (2) identify international groups of pedestrians based on psychosocial factors (i.e., Theory of Planned Behaviour (TPB) and perceptions of risk); and (3) investigate how segments of pedestrians form their intention for alcohol-impaired walking using the extended TPB (i.e. subjective norm, attitudes, perceived control, and perceived risk). A cross-sectional design was applied. The target behaviour question was "have you been a pedestrian when your thinking or physical ability (balance/strength) is affected by alcohol?" to ensure comparability across countries. Cluster analysis based on the extended TPB was used to identify groups of countries. Finally, regressions were used to predict pedestrians' intentions per group. A total of 6,166 respondents (Age M(SD) = 29.4 (14.2); Males = 39.2%) completed the questionnaire, ranging from 12.6% from Russia to 2.2% from Finland. The proportion of participants who reported never engaging in alcohol-impaired walking in the last three months ranged from 30.1% (Spain) to 83.1% (Turkey). Four groups of countries were identified: group-1 (Czech Republic, Spain, and Australia), group-2 (Russia and Finland), group-3 (Japan), and group-4 (final ten countries including Colombia, China, and Romania). Pedestrian intentions to engage in alcohol- impaired walking are predicted by perceptions of risk and TPB-psychosocial factors in group-1 and group-4. Favourable TPB-beliefs and low perceived risk increased alcohol-impaired walking intentions. Conversely, subjective norms were not significant in group-2 and only perceived risk predicted intention in group-3. The willingness of pedestrians to walk when alcohol-impaired differs significantly across the countries in this study. Perceived risk was the only common predictor among the 16 countries.
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http://dx.doi.org/10.1016/j.aap.2021.106212DOI Listing
September 2021

Temporal regulation of nicotinic acetylcholine receptor subunits supports central cholinergic synapse development in .

Proc Natl Acad Sci U S A 2021 Jun;118(23)

National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892;

The construction and maturation of the postsynaptic apparatus are crucial for synapse and dendrite development. The fundamental mechanisms underlying these processes are most often studied in glutamatergic central synapses in vertebrates. Whether the same principles apply to excitatory cholinergic synapses, such as those found in the insect central nervous system, is not known. To address this question, we investigated a group of projection neurons in the larval visual system, the ventral lateral neurons (LNvs), and identified nAchRα1 () and nAchRα6 () as the main functional nicotinic acetylcholine receptor (nAchR) subunits in the larval LNvs. Using morphological analyses and calcium imaging studies, we demonstrated critical roles of these two subunits in supporting dendrite morphogenesis and synaptic transmission. Furthermore, our RNA sequencing analyses and endogenous tagging approach identified distinct transcriptional controls over the two subunits in the LNvs, which led to the up-regulation of and down-regulation of during larval development as well as to an activity-dependent suppression of Additional functional analyses of synapse formation and dendrite dynamics further revealed a close association between the temporal regulation of individual nAchR subunits and their sequential requirements during the cholinergic synapse maturation. Together, our findings support transcriptional control of nAchR subunits as a core element of developmental and activity-dependent regulation of central cholinergic synapses.
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http://dx.doi.org/10.1073/pnas.2004685118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202021PMC
June 2021

Facet Selectivity Guided Assembly of Nanoarchitectures onto Two-Dimensional Metal-Organic Framework Nanosheets.

Angew Chem Int Ed Engl 2021 08 30;60(32):17564-17569. Epub 2021 Jun 30.

Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, School of Microelectronics, Wuhan University, Wuhan, 430072, China.

Facet-selective nanostructures in living systems usually exhibit outstanding optical and enzymatic properties, playing important roles in photonics, matter exchange, and biocatalysis. Bioinspired construction of facet-selective nanostructures offers great opportunities for sophisticated nanomaterials, but remains a formidable task. We have developed a macromolecule-mediated strategy for the assembly of upconversion nanoparticles (UCNPs)/two-dimensional metal-organic frameworks (2DMOFs) heterostructures with facet selectivity. Both experimental and theoretical results demonstrate that polyvinylpyrrolidone (PVP) can be utilized as an interface-selective mediator to further promote the facet-selective assembly of MOFs onto the surface of UCNPs. The UCNPs/2DMOFs nanostructures with facet selectivity display specific optical properties and show great advantages in anti-counterfeiting. Our demonstration of UCNPs/2DMOFs provides a vivid example for the controlled fabrication of facet-selective nanostructures and can promote the development of advanced functional materials for applications in biosensing, energy conversion, and information assurance.
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http://dx.doi.org/10.1002/anie.202103486DOI Listing
August 2021

Role of air scouring in anaerobic/anoxic tanks providing nitrogen removal by mainstream anammox conversion in a hybrid biofilm/suspended growth full-scale WWTP in China.

Water Environ Res 2021 May 26. Epub 2021 May 26.

Xi'an Wastewater Treatment Co. Ltd., Xi'an, China.

A full-scale wastewater treatment plant in China experienced unintentional anammox bacterial enrichment on biofilm carriers placed in the anaerobic and anoxic zones of an anaerobic/anoxic/oxic process under ambient temperatures and without bioaugmentation. Here, we show that microaerophilic conditions resulting from air scouring needed for biofilm carrier suspension in the anaerobic/anoxic zones can support a robust nitritation/anammox process. Results from an in situ on/off air scouring test showed that air scouring strongly induced both ammonia and total inorganic nitrogen removal in the anaerobic/anoxic zones. Ammonium concentration in the anaerobic and anoxic tanks remained constant or even slightly increased when air scouring was off, indicating that air scouring made a noticeable difference in nitrogen profiles in the anaerobic/anoxic zones. Various batch tests further indicated that partial denitrification is not likely to generate nitrite for anammox bacteria. Robust nitritation, and anammox on the carriers, can occur at low dissolved oxygen conditions, as measured in the full-scale facility. The observations show that mainstream deammonification without sidestream bioaugmentation at moderate temperature is feasible and further optimization by a more dedicated design can result in improved nitrogen removal in cases when chemical oxygen demand is limited in mainstream wastewater treatment. PRACTITIONER POINTS: Microaerophilic conditions in a full-scale IFAS reactor caused mainstream anammox in moderate temperate area. Robust nitritation, and anammox on the carriers, can occur at low dissolved oxygen conditions in anaerobic/anoxic tanks with air scouring. Anammox can function well with conventional nitrification and denitrification process at mainstream conditions for stable nitrogen removal.
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http://dx.doi.org/10.1002/wer.1592DOI Listing
May 2021

The application of XGBoost and SHAP to examining the factors in freight truck-related crashes: An exploratory analysis.

Accid Anal Prev 2021 Aug 23;158:106153. Epub 2021 May 23.

Urban Mobility Institute, Key Laboratory of Road and Traffic Engineering, Ministry of Education at Tongji University, College of Transportation Engineering, Tongji University, China. Electronic address:

Due to the burgeoning demand for freight movement, freight related road safety threats have been growing substantially. In spite of some research on the factors influencing freight truck-related crashes in major cities, the literature offers limited evidence about the effects of the built environment on the occurrence of those crashes by injury severity. This article uses data from the Los Angeles region in 2010-2019 to explore the relationships between the built environment factors and the spatial distribution of freight truck-related crashes using XGBoost and SHAP methods. Results from the XGBoost model show that variables related to the built environment, in particular demographics, land uses and road network, are highly correlated to freight truck related crashes of all three injury types. The SHAP value plots further indicate the important nonlinear relationships between independent variables and dependent variables. This study also emphasizes the differences in modeling mechanisms between the XGBoost model and traditional statistical models. The findings will help transport planners develop operational measures for resolving the emerging freight truck related traffic safety problems in local communities.
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http://dx.doi.org/10.1016/j.aap.2021.106153DOI Listing
August 2021

[Role of m A Reader YTHDC2 in Differentiation of Human Bone Marrow Mesenchymal Stem Cells].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 May;52(3):402-408

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Dental Implant, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Objective: To study the regulatory effect of YTH domain-containing protein 2 (YTHDC2), a member of N -methyladenosine (m A) readers, on the osteogenic or adipogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).

Methods: 2 expression was knocked down by small interfering RNA (siRNA) . Osteogenic differentiation and adipogenic differentiation of hBMSCs were induced after 2 knockdown in order to study the changes in the differentiation phenotype of hBMSCs. Alkaline phosphatase staining (ALP staining) and alizarin red S staining were performed to examine osteogenic activity and calcium-nodular formation. Nile red staining was performed to examine lipid-droplet formation. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to assess the expression of osteogenesis and adipogenesis-related genes. RNA-sequencing was performed to identify the transcriptome changes after 2 knockdown and to explore the potential regulatory mechanism by which YTHDC2 regulated the diferentiation of hBMSCs.

Results: In this study, we found that siRNA-induced 2 knockdown resulted in increased ALP activity and calcium-nodular formation of hBMSCs during osteogenic differentiation, and significantly upregulated the expression of osteogenesis-related genes. In addition, the lipid-droplet formation capacity of hBMSCs was decreased during adipogenic differentiation. The expression of adipogenesis-related genes was significantly down-regulated. Gene-set enrichmen analysis of RNA-seq data showed that YTHDC2 was significantly correlated with ribosome function and mRNA-translation-related signaling pathways.

Conclusion: The findings indicate that knockdown can promote the osteogenic differentiation of hBMSCs and inhibit the adipogenic differentiation. knockdown may cause changes in ribosome function.
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http://dx.doi.org/10.12182/20210560204DOI Listing
May 2021

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.

Theranostics 2021 3;11(13):6607-6615. Epub 2021 May 3.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, Fujian, P. R. China.

SARS-CoV-2 infection, which is responsible for the current COVID-19 pandemic, can cause life-threatening pneumonia, respiratory failure and even death. Characterizing SARS-CoV-2 pathogenesis in primary human target cells and tissues is crucial for developing vaccines and therapeutics. However, given the limited access to clinical samples from COVID-19 patients, there is a pressing need for models to investigate authentic SARS-CoV-2 infection in primary human lung cells or tissues with mature structures. The present study was designed to evaluate a humanized mouse model carrying human lung xenografts for SARS-CoV-2 infection . Human fetal lung tissue surgically grafted under the dorsal skin of SCID mice were assessed for growth and development after 8 weeks. Following SARS-CoV-2 inoculation into the differentiated lung xenografts, viral replication, cell-type tropism and histopathology of SARS-CoV-2 infection, and local cytokine/chemokine expression were determined over a 6-day period. The effect of IFN-α treatment against SARS-CoV-2 infection was tested in the lung xenografts. Human lung xenografts expanded and developed mature structures closely resembling normal human lung. SARS-CoV-2 replicated and spread efficiently in the lung xenografts with the epithelial cells as the main target, caused severe lung damage, and induced a robust pro-inflammatory response. IFN-α treatment effectively inhibited SARS-CoV-2 replication in the lung xenografts. These data support the human lung xenograft mouse model as a useful and biological relevant tool that should facilitate studies on the pathogenesis of SARS-CoV-2 lung infection and the evaluation of potential antiviral therapies.
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http://dx.doi.org/10.7150/thno.58321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224PMC
May 2021

Activity and structure of methanogenic microbial communities in sediments of cascade hydropower reservoirs, Southwest China.

Sci Total Environ 2021 Sep 4;786:147515. Epub 2021 May 4.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China. Electronic address:

Freshwater reservoirs are an important source of the greenhouse gas methane (CH). However, little is known about the activity and structure of microbial communities involved in methanogenic decomposition of sediment organic matter (SOM) in cascade hydropower reservoirs. In this study, we targeted on sediments of three cascade reservoirs in Wujiang River, Southwest China. Our results showed that the content of sediment organic carbon (SOC) was between 3% and 11%, and it's positively correlated with both C/N ratio and recalcitrant organic carbon content of SOM. Meanwhile, SOC content was positively correlated with CH production rates but had no significant correlation with total CO production rates of the sediments, when rates were normalized to sediment volume. Resultantly, the sediment anaerobic decomposition rates hardly significantly increase along with the SOC content. These results suggested that the terrestrial organic matter accumulated after damming stimulated CH production from the reservoir sediments even though its decomposition rate was limited. Meantime, high throughput sequencing of 16S rRNA genes indicated that not only the hydrogenotrophic and acetoclastic, but also the methylotrophic methanogens (Methanomassiliicoccus) are abundant in the reservoir sediments. Moreover, metagenomic sequencing also suggested that methylotrophic methanogenesis are potentially important in the sediment of cascade reservoirs. Finally, the hydraulic residence time of the reservoir could be the key controlling factor of the structures of bacterial and archaeal communities as well as the CH production rates of the reservoir sediments.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147515DOI Listing
September 2021
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