Publications by authors named "Yuan Qu"

143 Publications

Anti-scattering light focusing by fast wavefront shaping based on multi-pixel encoded digital-micromirror device.

Light Sci Appl 2021 Jul 20;10(1):149. Epub 2021 Jul 20.

Beijing Advanced Innovation Center for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Engineering Medicine, Beihang University, 100191, Beijing, China.

Speed and enhancement are the two most important metrics for anti-scattering light focusing by wavefront shaping (WS), which requires a spatial light modulator with a large number of modulation modes and a fast speed of response. Among the commercial modulators, the digital-micromirror device (DMD) is the sole solution providing millions of modulation modes and a pattern rate higher than 20 kHz. Thus, it has the potential to accelerate the process of anti-scattering light focusing with a high enhancement. Nevertheless, modulating light in a binary mode by the DMD restricts both the speed and enhancement seriously. Here, we propose a multi-pixel encoded DMD-based WS method by combining multiple micromirrors into a single modulation unit to overcome the drawbacks of binary modulation. In addition, to efficiently optimize the wavefront, we adopted separable natural evolution strategies (SNES), which could carry out a global search against a noisy environment. Compared with the state-of-the-art DMD-based WS method, the proposed method increased the speed of optimization and enhancement of focus by a factor of 179 and 16, respectively. In our demonstration, we achieved 10 foci with homogeneous brightness at a high speed and formed W- and S-shape patterns against the scattering medium. The experimental results suggest that the proposed method will pave a new avenue for WS in the applications of biomedical imaging, photon therapy, optogenetics, dynamic holographic display, etc.
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http://dx.doi.org/10.1038/s41377-021-00591-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292544PMC
July 2021

Efficient toluene adsorption/desorption on biochar derived from in situ acid-treated sugarcane bagasse.

Environ Sci Pollut Res Int 2021 Jul 1. Epub 2021 Jul 1.

School of Environmental Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.

Carbon-based materials with great adsorption performance are of importance to meet the needs of industrial gas adsorption. Having massive agricultural wastes of sugarcane bagasse, China could use this waste into wealth. However, the comprehensive utilization of sugarcane bagasse as precursor for biochar that can be used as adsorbent has not been extensively explored. In this study, a series of in situ sulfuric acid-modified biochar was prepared by hydrothermal carbonization process. The prepared biochar (SBAC-7) has a combination of two main advantages that are high microporosity (micropore surface area = 1106 m/g) and being rich in S-containing functional groups on the surface. In particular, SBAC-7 showed an excellent adsorption capacity of toluene (771.1 mg/g) at 30 °C, which is nearly 3 times as high as that of the commercial activated carbons. Meanwhile, it showed great stability and cyclic regeneration performance with five toluene adsorption-desorption test cycles. This study provides a high-performance biochar for the adsorption-desorption cycle in practical engineering applications, and would contribute to the sustainable "sugarcane production-bagasse utilization" circular economy.
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http://dx.doi.org/10.1007/s11356-021-15128-2DOI Listing
July 2021

Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation.

Carbohydr Polym 2021 Sep 18;268:118211. Epub 2021 May 18.

School of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming 650500, China. Electronic address:

This work explored the feasibility of using biological polysaccharide to fabricate dissolvable microneedles (MNs) for the purpose of transdermal drug delivery and skin dendritic cell (DC) activation. Panax notoginseng polysaccharide (PNPS), a naturally derived immunoactive macromolecule, was used to fabricate dissolvable MNs. The prepared PNPS MNs showed a satisfactory mechanical strength and a skin penetration depth. By Franz diffusion cell assay, the PNPS MNs demonstrated a high transdermal delivery amount of model drugs. Furthermore, with the assistance of MNs, PNPS easily penetrated across the stratum corneum and target ear skin DCs, activating the maturation and migration of immunocytes by increasing the expressions of CD40, CD80, CD86, and MHC II of skin DCs. Consequently, the matured DCs migrated to the auricular draining lymph nodes and increased the proportions of CD4+ T and CD8+ T cells. Thus, PNPS might be a promising biomaterial for transdermal drug delivery, with adjuvant potential.
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http://dx.doi.org/10.1016/j.carbpol.2021.118211DOI Listing
September 2021

Nanowire-structured FeP-CoP arrays as highly active and stable bifunctional electrocatalyst synergistically promoting high-current overall water splitting.

J Colloid Interface Sci 2021 Oct 18;600:811-819. Epub 2021 May 18.

Guangxi Key Laboratory of Low Carbon Energy Materials, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China. Electronic address:

The design and construction of highly efficient and durable non-noble metal bifunctional catalysts for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) in alkaline media is essential for developing the hydrogen economy. To achieve this goal, we have developed a bifunctional nanowire-structured FeP-CoP array catalyst on carbon cloth with uniform distribution through in-situ hydrothermal growth and phosphating treatment. The unique nanowire array structure and the strong electronic interaction between FeP and CoP species have been confirmed. Electrochemical studies have found that the designed FeCo-P/CC catalyst appears excellent HER (130 [email protected] mA cm)/OER (270 [email protected] mA cm) activity and stability. Moreover, the bifunctional FeCo-P/CC catalyst is also used in simulated industrial water splitting system, where the pair catalyst requires about 1.95 and 2.14 V to reach 500 and 1000 mA cm, even superior to the control RuO||Pt/C catalyst, showing good industrial application prospects. These excellent electrocatalytic properties are attributed to the synergy between FeP and CoP species as well as the unique microstructure, which can accelerate charge transfer, expose more active sites and enhance electrolyte diffusion and gas emissions.
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http://dx.doi.org/10.1016/j.jcis.2021.05.074DOI Listing
October 2021

Oxysterol-binding protein-like 2 contributes to the developmental progression of preadipocytes by binding to β-catenin.

Cell Death Discov 2021 May 17;7(1):109. Epub 2021 May 17.

Department of Medical Genetics, School of Basic Medical Science, Nanjing Medical University, Nanjing, China.

Oxysterol-binding protein-like 2 (OSBPL2), also known as oxysterol-binding protein-related protein (ORP) 2, is a member of lipid transfer protein well-known for its role in regulating cholesterol homeostasis. A recent study reported that OSBPL2/ORP2 localizes to lipid droplets (LDs) and is associated with energy metabolism and obesity. However, the function of OSBPL2/ORP2 in adipocyte differentiation is poorly understood. Here, we report that OSBPL2/ORP2 contributes to the developmental progression of preadipocytes. We found that OSBPL2/ORP2 binds to β-catenin, a key effector in the Wnt signaling pathway that inhibits adipogenesis. This complex plays a role in regulating the protein level of β-catenin only in preadipocytes, not in mature adipocytes. Our data further indicated that OSBPL2/ORP2 mediates the transport of β-catenin into the nucleus and thus regulates target genes related to adipocyte differentiation. Deletion of OSBPL2/ORP2 markedly reduces β-catenin both in the cytoplasm and in the nucleus, promotes preadipocytes maturation, and ultimately leads to obesity-related characteristics. Altogether, we provide novel insight into the function of OSBPL2/ORP2 in the developmental progression of preadipocytes and suggest OSBPL2/ORP2 may be a potential therapeutic target for the treatment of obesity-related diseases.
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http://dx.doi.org/10.1038/s41420-021-00503-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129138PMC
May 2021

Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.

J Ethnopharmacol 2021 Aug 6;276:114189. Epub 2021 May 6.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; Key Laboratory of Sustainable Utilization of Panax notoginseng Resources of Yunnan Province, Kunming 650500, China. Electronic address:

Ethnopharmacological Relevance: Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years. However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.

Aims Of The Study: This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.

Materials And Methods: Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP. The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups. The CKD mouse model was established by the adenine gavage. The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining. Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP. Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.

Results: OLP is composed of three kinds of monosaccharides. There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage. OLP could significantly attenuate CKD in mice and the tubulointerstitial damage was recovered to almost normal after the treatment of OLP. Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment. Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.

Conclusions: OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide. OLP could ameliorate CKD in mice by declining the oxidative stress, inflammation, apoptosis and fibrosis in the kidneys. The study provided some evidences for the potential application of OLP in alleviating CKD.
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http://dx.doi.org/10.1016/j.jep.2021.114189DOI Listing
August 2021

Stage-dependent conditional survival and failure hazard of non-metastatic nasopharyngeal carcinoma after intensity-modulated radiation therapy: Clinical implications for treatment strategies and surveillance.

Cancer Med 2021 Jun 6;10(11):3613-3621. Epub 2021 May 6.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Purpose: Conditional survival (CS) and failure hazard estimations can provide important dynamic prognostic information for clinical decision-making and surveillance counseling. The current study aimed to investigate the CS and dynamic failure hazard in non-metastatic nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT).

Methods: Conditional overall survival (COS) and progression-free survival (CPFS) estimates adjusted for age and gender against each AJCC 8th stage were calculated. Multivariable Cox regression (MCR) models were fitted in the entire population at baseline and subsequently separate MCR models were fitted in patients who have maintained event-free time of 1 to 10 years to generate respective hazard ratio (HR). Annual hazard rates of death and progression over 10 years for each stage were also estimated.

Results: A total of 1993 patients were eligible for analysis. The estimated 5-year OS and PFS for entire cohort were 79.0% and 70.7% at initial diagnosis. After 5 years of event-free follow-up, additional 5-year COS and CPFS increased to 85.9% and 85.5%, respectively. Stage I/II maintained dramatically favorable CS and low hazard (< 5%) of death and progression over time. Relative to stage I/II, stage III manifested non-significantly higher failure hazard for the first 3 years of survivorship and approached to similar level of stage I/II afterwards. Stage IVA presented most impressive improvement in terms of both COS (∆=9.8%) and CPFS (∆ = 16.8%) whereas still drastically inferior to that of stage I-III across all conditional time points. After 4 years of follow-up, progression hazard of stage IVA became relatively steady of approximate 6%.

Conclusions: Survival prospect of non-metastatic NPC improves over years with distinct dynamic patterns across stages, providing important implications for personalized decision-making in terms of both clinical management and surveillance counseling. Stage-dependent and hazard-adapted clinical management and surveillance are warranted.
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http://dx.doi.org/10.1002/cam4.3917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178506PMC
June 2021

Identification and quantification of oleanane triterpenoid saponins and potential analgesic and anti-inflammatory activities from the roots and rhizomes of .

J Ginseng Res 2021 Mar 18;45(2):305-315. Epub 2020 May 18.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

Background: represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from .

Methods: The chemical characterization of allowed the identification and quantitation of two major compounds. Analgesic effects of triterpenoid saponins were evaluated in two models of thermal- and chemical-stimulated acute pain. Anti-inflammatory effects of triterpenoid saponins were also evaluated using four models of acetic acid-induced vascular permeability, xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet-induced granuloma in mice.

Results: Two triterpenoid saponins of stipuleanosides R (SP-R) and R (SP-R) were isolated and identified from The results showed that SP-R and SP-R significantly increased the latency time to thermal pain in the hot plate test and reduced the writhing response in the acetic acid-induced writhing test. SP-R and SP-R caused a significant decrease in vascular permeability, ear edema, paw edema, and granuloma formation in inflammatory models. Further studies showed that the levels of inflammatory mediators, nitric oxide, malondialdehyde, tumor necrosis factor-α, and interleukin 6 in paw tissues were downregulated by SP-R and SP-R. In addition, the rational harvest of three- to five-year-old was preferable to obtain a higher level of triterpenoid saponins. SP-R showed the highest content in , which had potential as a chemical marker for quality control of .

Conclusion: This study provides important basic information about utilization of resources for production of active triterpenoid saponins.
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http://dx.doi.org/10.1016/j.jgr.2020.05.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020355PMC
March 2021

Neurometabolic alterations in bipolar disorder with anxiety symptoms: A proton magnetic resonance spectroscopy study of the prefrontal whiter matter.

Psychiatry Res 2021 May 8;299:113859. Epub 2021 Mar 8.

Department of Radiology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830000, China.

To identify the pathophysiological mechanism of bipolar disorder (BD) patients with anxiety symptoms, we analyzed the differences of brain biochemical metabolism in BD patients with and without anxiety symptoms. We collected 39 BD patients who had been untreated with drugs in one month and were divided into the anxiety symptoms group (20 cases) and non-anxiety symptoms group (19 cases) according to whether they had anxiety symptoms. We used proton magnetic resonance spectroscopy (H-MRS) to detect the biochemical metabolite ratios of the prefrontal whiter matter (PWM) in all patients. The right PWM mI/Cr ratios in BD patients with anxiety symptoms were higher than those in BD patients without anxiety symptoms and the Cho/Cr ratios in the left PWM were negatively correlated with age and age of onset in BD patients with anxiety symptoms. These findings indicated that BD patients with anxiety symptoms have increased levels of inositol metabolism in the right PWM. Furthermore, the level of membrane phospholipid catabolism in the left PWM of BD patients with anxiety symptoms decreased with increasing age and onset age. Our results provide some references for the pathophysiological mechanism in BD patients with anxiety symptoms.
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http://dx.doi.org/10.1016/j.psychres.2021.113859DOI Listing
May 2021

Low- and high-dose radioiodine ablation for low-/intermediate-risk differentiated thyroid cancer in China: Large randomized clinical trial.

Head Neck 2021 04 19;43(4):1311-1320. Epub 2021 Jan 19.

Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, China.

Background: To determine whether 1.1 GBq radioiodine therapy is as effective as 3.7 GBq for ablation in Chinese patients with differentiated thyroid cancer (DTC).

Methods: In this single-center randomized study, we compared the successful radioiodine ablation rates of 1.1 GBq and 3.7 GBq for patients with DTC.

Results: At 6-8 months after radioiodine ablation, there were 95 (39%) patients in the 1.1 GBq group and 79 (32%) patients in the 3.7 GBq group with thyroid hormone withdrawal (THW), and ablation success rates were 84% versus 80%, respectively; and 149 (61%) patients in the 1.1 GBq group and 169 (68%) patients in the 3.7 GBq group without THW, and ablation success rates were 89% versus 90%, respectively. In total, the ablation was successful in 412 (87%) of the 474 patients, and it was similar between the two groups.

Conclusions: Low-dose radioiodine ablation was as effective as high dose in Chinese DTC patients.
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http://dx.doi.org/10.1002/hed.26594DOI Listing
April 2021

Proposal of a TNM classification-based staging system for esthesioneuroblastoma: More precise prediction of prognosis.

Head Neck 2021 04 10;43(4):1097-1104. Epub 2020 Dec 10.

Department of Radiation Oncology, National Cancer Center/ National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Esthesioneuroblastoma (ENB) is a rare malignant neoplasm. Currently, no consistent and universal staging system for ENB exists. The aim of this study is to propose a TNM-based classification.

Subjects And Methods: Hundred and forty-two patients from our institution, with ENB pathologically confirmed between July 1978 and December 2018, were reviewed. All patients were restaged according to the Kadish stage, Morita stage and American Joint Committee on Cancer (AJCC) T classification from clinical and radiological data. Multivariate Cox proportional hazard regression analyses were performed to determine the impact of various factors. The goodness-of-fit and predictive accuracy of the different staging systems were calculated using R software.

Results: The median follow-up time was 57 months (range: 4-229 months). According to the Kadish system, the 5-year overall survival (OS) for patients with stage A, B and C was 100%, 83.6% and 64.2%, respectively (P = .055). With respect to the Morita classification, 5-year OS for stages A, B, C and D was 100%, 83.6%, 70.7% and 50.0%, respectively (P = .004). Analysis based on the proposed staging model demonstrated 5-year OS for stage I, II, III and IV disease was 100%, 88.9%, 75.9% and 49.0%, respectively (P < .001). In separate multivariate Cox regression models, only the novel staging system exhibited independent effects on OS (P = .004); the Akaike information criterion and Harrell's concordance index were also superior to those calculated for the Kadish or Morita systems.

Conclusions: The proposed TNM-based staging system offers an improved prognostic assessment for patients with ENB. Further verification and refinement from additional dataset application is required.
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http://dx.doi.org/10.1002/hed.26559DOI Listing
April 2021

Kirenol inhibits RANKL-induced osteoclastogenesis and prevents ovariectomized-induced osteoporosis via suppressing the Ca-NFATc1 and Cav-1 signaling pathways.

Phytomedicine 2021 Jan 12;80:153377. Epub 2020 Oct 12.

Laboratory of Anti-inflammatory and Immunomodulatory Pharmacology, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515,China. Electronic address:

Background: Osteoporosis is a threat to aged people who have excessive osteoclast activation and bone resorption, subsequently causing fracture and even disability. Inhibiting osteoclast differentiation and absorptive functions has become an efficient approach to treat osteoporosis, but osteoclast-targeting inhibitors available clinically remain rare. Kirenol (Kir), a bioactive diterpenoid derived from an antirheumatic Chinese herbal medicine Herba Siegesbeckiae, can treat collagen-induced arthritis in vivo and promote osteoblast differentiation in vitro, while the effects of Kir on osteoclasts are still unclear.

Purpose: We explore the role of Kir on RANKL-induced osteoclastogenesis in vitro and bone loss in vivo.

Methods: The in vitro effects of Kir on osteoclast differentiation, bone resorption and the underlying mechanisms were evaluated with bone marrow-derived macrophages (BMMs). In vivo experiments were performed using an ovariectomy (OVX)-induced osteoporosis model.

Results: We found that Kir remarkably inhibited osteoclast generation and bone resorption in vitro. Mechanistically, Kir significantly inhibited F-actinring formation and repressed RANKL-induced NF-κB p65 activation and p-p38, p-ERK and c-Fos expression. Moreover, Kir inhibited both the expression and nuclear translocation of NFATc1. Ca oscillation and caveolin-1 (Cav-1) were also reduced by Kir during osteoclastogenesis in vitro. Consistent with these findings, 2-10 mg/kg Kir attenuated OVX-induced osteoporosis in vivo as evidenced by decreased osteoclast numbers and downregulated Cav-1 and NFATc1 expression.

Conclusions: Kir suppresses osteoclastogenesis and the Cav-1/NFATc1 signaling pathway both in vitro and in vivo and protects against OVX-induced osteoporosis. Our findings reveal Kir as a potential safe oral treatment for osteoporosis.
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http://dx.doi.org/10.1016/j.phymed.2020.153377DOI Listing
January 2021

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe.

Chem Commun (Camb) 2020 Nov;56(90):14075-14078

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China.

Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.
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http://dx.doi.org/10.1039/d0cc05900eDOI Listing
November 2020

Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy.

Carbohydr Polym 2020 Dec 13;250:116904. Epub 2020 Aug 13.

School of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, 650500, China. Electronic address:

In the current study, we developed a synergistic chemo-immunotherapy using doxorubicin (Dox) and a natural polysaccharide as immunomodulator. First, we isolated a polysaccharide (MPW) from the root of Lepidium meyenii Walp. (maca) and characterized its chemical properties. MPW contains → 4) -α-D-Glcp- (1 → glycosidic bonds, while the terminal α-D-Glcp- (1 → group is connected to the main chain through an O-6 bond. This polysaccharide was then modified by cationization (C-MPW) to enhance immunoregulatory activity. MPW and C-MPW were combined with Dox and their chemo-immunotherapy effects on 4T1 tumor-bearing mice were assessed. Results indicated that the combination of MPW/C-MPW exerted a stronger anti-tumor effect than Dox alone, while reducing systemic toxicity and inhibiting tumor metastasis. In addition, MPW and C-MPW exerted tumor immunotherapy effects through the NF-κB, STAT1, and STAT3 signaling pathways, redirecting TAMs to the M1 phenotype that facilitates immunological responses against tumors. As a result, the immunosuppressive tumor microenvironment was remodeled into an immune-activated state due to enhanced secretion of IL-12, TNF-α, and INF-γ. Moreover, C-MPW exerted a stronger immunomodulatory effect than MPW. In conclusion, MPW and its cationic derivative are promising tools for cancer immunotherapy.
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http://dx.doi.org/10.1016/j.carbpol.2020.116904DOI Listing
December 2020

Patterns of Cervical Lymph Node Metastasis in Locally Advanced Supraglottic Squamous Cell Carcinoma: Implications for Neck CTV Delineation.

Front Oncol 2020 27;10:1596. Epub 2020 Aug 27.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: To investigate the prevalence and distribution of cervical lymph node metastasis (LNM) in locally advanced supraglottic squamous cell carcinoma (LASCC) and guide the delineation of clinical lymph node target volumes.

Materials And Methods: We reviewed patients defined as LASCC from January 2000 to December 2017 in our hospital. The primary tumor was operated on using partial or total laryngectomy, and all patients underwent bilateral neck dissection (levels II-IV at least). Univariate and multivariate logistic regressions were used to find risk factors associated with LNM.

Results: A total of 206 patients were enrolled. In the whole group, the rate of ipsilateral metastasis (IM) was 60.9% (67 patients), whereas contralateral metastasis was 25.5% (28 patients). Only positive ipsilateral lymph nodes contributed to contralateral metastasis ( = 0.001). Seventy-six cases were diagnosed with clinical positive lymph nodes (cN). IM of primary lesions mainly located within the unilateral sites ( = 49 patients) was detected in levels II, III, and IV with lymph node metastasis ratios of 73.5% (36 patients), 63.3% (31 patients), and 20.4% (10 patients), respectively, and contralateral metastasis of 36.7% (18 patients), 16.3% (8 patients), and 6.1% (3 patients), respectively. Involvement of level II or III was associated with metastasis of level IV. No one developed contralateral level IV involvement without metastasis of contralateral levels II and III. A total of 130 cases had clinically negative neck lymph nodes (cN0). The prevalence of occult metastasis (OM) was 35.4%. Among 62 patients with unilateral lesions, the rates of OM to ipsilateral neck levels II, III, and IV were 21, 11.1, and 1.6%, respectively, whereas contralateral neck levels were 6.3, 4.8, and 0%, respectively. In terms of the risk factors, histopathological differentiation was related to OM ( = 0.003). Two of 25 people were with level VIb metastasis, and both of them were with subglottic involvement.

Conclusion: Neck levels II to IV are most frequently involved and should be included in clinical target volume (CTV) in cN patients. Contralateral IV may be omitted when contralateral levels II and III are negative. In cN0 patients, ipsilateral levels II and III are suggested to be included in the CTV, whereas whether contralateral levels II and III should be included needs further research.
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http://dx.doi.org/10.3389/fonc.2020.01596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481353PMC
August 2020

The Pattern of Cervical Lymph Node Metastasis and Risk Factors of Retropharyngeal Lymph Node Metastasis Based on Magnetic Resonance Imaging in Different Sites of Hypopharyngeal Carcinoma.

Cancer Manag Res 2020 18;12:8581-8587. Epub 2020 Sep 18.

National Cancer Center/National Cancer Clinical Medical Research Center/Chinese Academy of Medical Sciences, Peking Union Medical College, Cancer Hospital, Beijing, People's Republic of China.

Background: This study was to determine the patterns of regional lymph node (LN) spread and the risk factors of retropharyngeal lymph node (RPLN) metastasis based on magnetic resonance imaging (MRI) in hypopharyngeal squamous carcinoma (HPC) to improve clinical target volume (CTV) delineation.

Methods: A cohort of 326 consecutive patients of HPC in a single institute were retrospectively reviewed. All patients underwent MRI prior to initial treatment, and the diagnosis based on MRI of the LN metastasis was confirmed by all radiation oncologists in the head and neck group during twice weekly chat rounds. Statistical analysis of data was using chi-square test and multivariant logistic regression model in SPSS 22.0 software.

Results: The LN metastasis rate of all patients in this cohort was 90.5% (295/326). Level IIa/b and level III were the most frequently involved regions followed by level IV and retropharyngeal region. Skip metastasis only occurred in 6.4% (19/295). Univariate and multivariate analysis demonstrated that primary tumor subsites were located in the posterior pharyngeal wall (=0.002), bilateral cervical LN metastasis (=0.020), larger volume of primary gross target (GTVp, =0.003), and larger volume of LN gross target (GTVnd, =0.023) were significantly associated with RPLN metastasis.

Conclusion: The regional LN spread of HPC follows an ordered pattern as level II is the most frequently involved area followed by level III, level IV, and RPLN. RPLN metastasis is more likely to occur in patients with primary site of posterior pharyngeal wall, large tumor burden, or bilateral neck LN metastasis. Therefore, it is highly recommended that the RPLN should be included into CTV for patients who have these risk factors.
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http://dx.doi.org/10.2147/CMAR.S245988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511603PMC
September 2020

Long-term analysis of multimodality treatment outcomes and prognosis of esthesioneuroblastomas: a single center results of 138 patients.

Radiat Oncol 2020 Sep 18;15(1):219. Epub 2020 Sep 18.

Department of Radiation Oncology, National Cancer Center/ National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Background: The aim of this study is to evaluate the efficacy of different treatment strategies and the potential prognostic factors of esthesioneuroblastoma (ENB).

Materials And Methods: Between April 1984 and December 2018, 138 patients with non-metastatic ENB were retrospectively analyzed. The treatment modalities mainly included surgery alone (n = 7), radiotherapy alone (n = 33), concurrent chemoradiotherapy (n = 17), surgery combined with current chemoradiotherapy (n = 32), and surgery plus radiotherapy (n = 49).

Results: The median follow-up time for the entire cohort was 61 months (range, 4-231 months). The 5-year overall survival (OS), locoregional failure-free survival (LRFFS), and distant metastasis-free survival (DMFS) rate were 69.6, 78.0 and 73.9%, respectively. Surgery combined with radiotherapy elicited superior survival results, and the combination of surgery and current chemoradiotherapy achieved the best prognoses for all patients, patients with advanced Kadish disease, patients receiving intensity modulated radiation therapy and those with positive surgical margin. Univariate analysis identified orbital invasion and treatment modalities were predictors for OS, LRFFS and DMFS. Lymph node metastasis was associated with OS and DMFS, but not LRFFS. Intracranial invasion, advanced Kadish stage and not receiving concurrent chemotherapy were also predictive of lower OS. Multivariate analyses indicated that lymph node metastasis was an independent prognostic factor affecting DMFS, whereas treatment modalities was independent prognostic factors for OS and LRFFS.

Conclusion: Orbital invasion, intracranial invasion, lymph node metastasis and advanced Kadish disease at initial diagnosis were significantly associated with inferior prognosis. Regarding the treatment modality, the optimal strategy remined surgery with radiotherapy-based multimodality treatment. The concurrent chemoradiotherapy may play a more beneficial role.
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http://dx.doi.org/10.1186/s13014-020-01667-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501706PMC
September 2020

Correlation between bone turnover and metabolic markers with age and gender: a cross-sectional study of hospital information system data.

BMC Musculoskelet Disord 2020 Sep 10;21(1):603. Epub 2020 Sep 10.

Department of Rheumatology and Clinical Immunology, ZhuJiang Hospital, Southern Medical University, 253 Gongye Ave, Guangzhou, 510282, Guangdong, China.

Background: Bone turnover and metabolic indicators are related to age and gender. Age and gender should be matched in subjects in disease control research of bone turnover and metabolism, but strict matching of gender and age increases the difficulty and cost of the research. Therefore, the aim of this study was to solve it is necessary to strictly match age and gender in clinical research in bone metabolism.

Methods: A cross-sectional study was conducted from the data were extracted from the HIS of ZhuJiang Hospital. Data relating to seven bone turnover and metabolic indicators from 1036 patients between January 2018 and October 2019 were analyzed.

Results: P1NP, β-CTx and 25(OH)D were significant different in individuals younger than 20 years of age. ALP was significantly higher in those under 20 years of age and lower at age 20-39 compared with other age groups. The concentrations of Ca and P were different among the groups aged 0-19, 20-39, and 40-59 years of age groups but exhibited no difference above 60 years of age. PTH expression was not dependent on age. P1NP, β-CTx and PTH concentrations were not significantly different between the genders within the same age group. ALP was significantly different between genders within the age range 20-59 years. Ca and 25(OH)D were significantly different between the genders for those older than 60. Serum P was significantly different in the two genders for those aged 40-79. Patients received both alfacalcidol and calcium treatment differently from the others in P1NP, β-CTx, Serum Ca, P and ALP.

Conclusion: P1NP and β-CTx were highly correlated with age. If these two indictors require analysis in a case control study, the patients and controls should be strictly matched by age under 20 years. The demarcation point for ALP was 40 years of age. Ca and P were strongly recommended strict matching according to age in disease research. The difference in P1NP, β-CTx, 25(OH)D and ALP between genders depends on age differences. Medication history should be considered in bone turnover and metabolic clinical research.
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http://dx.doi.org/10.1186/s12891-020-03610-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487991PMC
September 2020

Adding Concurrent Chemotherapy to Intensity-Modulated Radiotherapy Does Not Improve Treatment Outcomes for Stage II Nasopharyngeal Carcinoma: A Phase 2 Multicenter Clinical Trial.

Front Oncol 2020 7;10:1314. Epub 2020 Aug 7.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

To explore the efficacy of concomitant chemotherapy in intensity-modulated radiotherapy (IMRT) to treat stage II nasopharyngeal carcinoma (NPC). In this randomized phase 2 study [registered with ClinicalTrials.gov (NCT01187238)], eligible patients with stage II (2010 UICC/AJCC) NPC were randomly assigned to either IMRT alone (RT group) or IMRT combined with concurrent cisplatin (40 mg/m, weekly) (CCRT group). The primary endpoint was overall survival (OS). The second endpoints included local failure-free survival (LFFS), regional failure-free survival (RFFS), disease-free survival (DFS), distant metastasis-free survival (DMFS), and acute toxicities. Between May 2010 to July 2012, 84 patients who met the criteria were randomized to the RT group ( = 43) or the CCRT group ( = 41). The median follow-up time was 75 months. The OS, LFFS, RFFS, DFS, and DMFS for the RT group and CCRT group were 100% vs. 94.0% ( = 0.25), 93.0% vs. 89.3% ( = 0.79), 97.7% vs. 95.1% ( = 0.54), 90.4% vs. 86.6% ( = 0.72), and 95.2% vs. 94.5% ( = 0.77), respectively. A total of 14 patients experienced disease failure, 7 patients in each group. The incidence of grade 2 to 4 leukopenia was higher in the CCRT group ( = 0.022). No significant differences in liver, renal, skin, or mucosal toxicity was observed between the two groups. For patients with stage II NPC, concomitant chemotherapy with IMRT did not improve survival or disease control but had a detrimental effect on bone marrow function.
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http://dx.doi.org/10.3389/fonc.2020.01314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426506PMC
August 2020

Rno_circ_0005139 regulates apoptosis by targeting in rat anorectal malformations.

World J Gastroenterol 2020 Aug;26(29):4272-4287

Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: The molecular mechanisms underlying anorectal malformations (ARM) are not fully established. Circular RNAs (circRNAs) are new born non-coding RNAs, and their role in ARM is unclear. We assumed that rno_circ_0005139 influences apoptosis and proliferation by acting as a miR-324-3p sponge, and downregulating in ARM.

Aim: To identify the differential expression of circRNAs and mRNAs in a rat ARM model.

Methods: Sixty-six pregnant Wistar rats were randomly divided into two groups: ARM group (2-imidazolidinethione-induced) and control groups. Embryos were harvested by cesarean delivery, and anorectal tissue was taken on embryonic days 16 (E16), 17 (E17), 19 (E19), and 21 (E21). RNA sequencing and gene microarray analysis was used to identify differentially expressed circRNAs and mRNAs in the ARM in a rat model. We selected 6 circRNAs and 3 mRNAs in the Wnt signal pathway from the result of the RNA sequencing and gene microarray analysis, and quantitative reverse transcription polymerase chain reaction was performed to evaluate their tissue expression. According to bioinformatics prediction, rno_circ_0005139 acted as a miR-324-3p sponge to regulate the expression of . We chose rno_circ_0005139 and Wnt5a as the final candidates. We tested the function of rno_circ_0005139 and the binding sites between rno_circ_0005139 and miR-324-3p, miR-324-3p and by luciferase assays. Co-transfection of rno_circ_0005139 and miR-324-3p was to verify their functional consistency.

Results: We identified 38 upregulated and 42 downregulated circRNAs on E17 ( < 0.05), and 301 mRNAs were upregulated and 256 downregulated in the ARM on E17 ( < 0.05, fold-change > 2.0). We found that rno_circ_0006880 and rno_circ_0011386 were upregulated, whereas rno_circ_0000436, rno_circ_0005139, rno_circ_0009285, rno_circ_0014367, , , and were downregulated in ARM tissues. According to bioinformatics prediction, rno_circ_0005139 acted as a miR-324-3p sponge to regulate the expression of . We chose rno_circ_0005139 and as the final candidates. Because the role and molecular mechanism of rno_circ_0005139 are poorly understood, its effect on apoptosis and proliferation was investigated by plasmid transfection. A luciferase experiment showed that rno_circ_0005139 could bind with miR-324-3p, which negatively regulated expression. The expression of miR-324-3p was significantly higher in ARM anorectal tissues than that in control group on E17 and E19; expression showed the opposite trend. In addition, a miR-324-3p inhibitor attenuated the effects of rno_circ_0005139 knockdown on ARM development.

Conclusion: Rno_circ_0005139 influences cell proliferation and apoptosis by acting as a miR-324-3p sponge, thereby downregulating in ARM. Accordingly, rno_circ_0005139, miR-324-3p, and could be targeted therapeutic factors for ARM.
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http://dx.doi.org/10.3748/wjg.v26.i29.4272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7422537PMC
August 2020

A Dihydroflavonoid Naringin Extends the Lifespan of . and Delays the Progression of Aging-Related Diseases in PD/AD Models via DAF-16.

Oxid Med Cell Longev 2020 31;2020:6069354. Epub 2020 Jul 31.

Key Laboratory for Aging and Regenerative Medicine, Department of Pharmacology School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.

Naringin is a dihydroflavonoid, which is rich in several plant species used for herbal medicine. It has a wide range of biological activities, including antineoplastic, anti-inflammatory, antiphotoaging, and antioxidative activities. So it would be interesting to know if naringin has an effect on aging and aging-related diseases. We examined the effect of naringin on the aging of (. ). Our results showed that naringin could extend the lifespan of . . Moreover, naringin could also increase the thermal and oxidative stress tolerance, reduce the accumulation of lipofuscin, and delay the progress of aging-related diseases in . models of AD and PD. Naringin could not significantly extend the lifespan of long-lived mutants from genes in insulin/IGF-1 signaling (IIS) and nutrient-sensing pathways, such as -, -, -, -, -., and -. Naringin treatment prolonged the lifespan of long-lived - mutants, which have decreased reproductive stem cells. Naringin could not extend the lifespan of a null mutant of the fox-head transcription factor DAF-16. Moreover, naringin could increase the mRNA expression of genes regulated by - and itself. In conclusion, we show that a natural product naringin could extend the lifespan of . and delay the progression of aging-related diseases in . models via DAF-16.
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http://dx.doi.org/10.1155/2020/6069354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7422489PMC
June 2021

Novel ginsenoside derivative 20(S)-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism.

Cell Death Dis 2020 08 14;11(8):621. Epub 2020 Aug 14.

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China.

Increased energy metabolism is responsible for supporting the abnormally upregulated proliferation and biosynthesis of cancer cells. The key cellular energy sensor AMP-activated protein kinase (AMPK) and the glycolytic enzyme alpha-enolase (α-enolase) have been identified as the targets for active components of ginseng. Accordingly, ginseng or ginsenosides have been demonstrated with their potential values for the treatment and/or prevention of cancer via the regulation of energy balance. Notably, our previous study demonstrated that the R-form derivative of 20(R)-Rh2, 20(R)-Rh2E2 exhibits specific and potent anti-tumor effect via suppression of cancer energy metabolism. However, the uncertain pharmacological effect of S-form derivative, 20(S)-Rh2E2, the by-product during the synthesis of 20(R)-Rh2E2 from parental compound 20(R/S)-Rh2 (with both R- and S-form), retarded the industrialized production, research and development of this novel effective candidate drug. In this study, 20(S)-Rh2E2 was structurally modified from pure 20(S)-Rh2, and this novel compound was directly compared with 20(R)-Rh2E2 for their in vitro and in vivo antitumor efficacy. Results showed that 20(S)-Rh2E2 effectively inhibited tumor growth and metastasis in a lung xenograft mouse model. Most importantly, animal administrated with 20(S)-Rh2E2 up to 320 mg/kg/day survived with no significant body weight lost or observable toxicity upon 7-day treatment. In addition, we revealed that 20(S)-Rh2E2 specifically suppressed cancer cell energy metabolism via the downregulation of metabolic enzyme α-enolase, leading to the reduction of lactate, acetyl-coenzyme (acetyl CoA) and adenosine triphosphate (ATP) production in Lewis lung cancer cells (LLC-1), but not normal cells. These findings are consistent to the results obtained from previous studies using a similar isomer 20(R)-Rh2E2. Collectively, current results suggested that 20(R/S)-Rh2E2 isomers could be the new and safe anti-metabolic agents by acting as the tumor metabolic suppressors, which could be generated from 20(R/S)-Rh2 in industrialized scale with low cost.
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http://dx.doi.org/10.1038/s41419-020-02881-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427995PMC
August 2020

Long-term outcomes of patients in different histological subtypes of primary nasopharyngeal adenocarcinoma: A single-center experience with 71 cases.

Oral Oncol 2020 12 11;111:104923. Epub 2020 Aug 11.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. Electronic address:

Objective: This study is aimed to evaluate the long-term outcomes and management approaches in different histological subtypes of primary nasopharyngeal adenocarcinoma (NPAC).

Materials And Methods: 71 patients with NPAC at our institution between 1984 and 2016 were reviewed, including adenoid cystic carcinoma (ACC) in 43 patients, mucoepidermoid carcinoma (MEC) in 17 patients, and primary traditional adenocarcinoma (AC) in 11 patients. 37 patients received primary radiotherapy and 34 patients underwent primary surgery.

Results: The median time of follow-up was 77 months. The 5-year overall survival rate (OS), locoregional failure-free survival rate (LRFFS) and distant metastasis failure-free survival rate (DFFS) were 69.9%, 67.1% and 77.9%, respectively. Patients who received combined modality therapy had better 5-year OS (73.7% vs 66.2%, p = 0.065) and LRFFS (73.1% vs 64.5%, p = 0.047) than patients receiving single modality therapy. Regarding the different histological subtypes, the survival rates of patients with ACC undergoing primary radiotherapy and primary surgery were similar (5-year OS 82.3% vs 68.8%, LRFFS 70.0% vs 70.8%, p>0.05). As to patients with MEC and AC, those who underwent primary surgery achieved better 5-year OS (75.6% vs 45.5%, p = 0.001) and LRFFS (70.6%% vs 57.1%, p = 0.014) than those who received primary radiotherapy. Multivariate analyses indicated that histological subtypes and radiotherapy technique were independent factors for OS.

Conclusions: The optimal treatment policy for NPAC remained the combination of radiotherapy and surgery. For patients with ACC, radiotherapy could be considered as the primary treatment. Surgery was suggested to be the primary treatment in patients with MEC and AC.
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http://dx.doi.org/10.1016/j.oraloncology.2020.104923DOI Listing
December 2020

Correlation of Myopia with Physical Exercise and Sleep Habits among Suburban Adolescents.

J Ophthalmol 2020 25;2020:2670153. Epub 2020 Jul 25.

Department of Physical Examination, Zhujiajiao People's Hospital of Qingpu District, Shanghai, China.

Purpose: To analyse the correlation of myopia with physical exercise and sleep habits to explore the possible risk or protective factors of juvenile myopia.

Methods: This study was a retrospective cross-sectional study. A total of 1831 students aged 11-18 years from five schools in Qingpu completed questionnaires from 2018 to 2019; the students were divided into the nonmyopia group and the myopia group, with students in the myopia group having myopia in at least one eye. Based on the chi-square test, the variables with statistical significance were selected for the logistic regression analysis. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were determined.

Results: Myopia was positively associated with older age (OR = 2.216, 95% CI: 1.720-2.855), having a parent with myopia (father: OR = 2.463, 95% CI: 1.900-3.193; mother: OR = 2.853, 95% CI: 2.232-3.646), and a late bedtime during holidays (before 10 pm:  = 0.002; 10 pm-11 pm: OR = 1.516, 95% CI: 1.095-2.100; 11 pm-12 am: OR = 1.966, 95% CI: 1.383-2.795), but negatively correlated with male gender (OR = 0.693, 95% CI: 0.549-0.876), a late daily wake time (OR = 0.782, 95% CI: 0.621-0.985), and having more days per week of outdoor activities during the semester (OR = 0.764, 95% CI: 0.597-0.978). After adjustment for demographic characteristics, myopia was positively associated with a late bedtime during holidays (before 10 pm:  = 0.003; 10 pm-11 pm: OR = 1.570, 95% CI: 1.076-2.289; 11 pm-12 am: OR = 2.173, 95% CI: 1.451-3.253; after 12 am: OR = 1.786, 95% CI: 1.093-2.919), but negatively correlated with a late daily wake time during the semester (OR = 0.761, 95% CI: 0.581-0.997).

Conclusion: The association of outdoor exercise with myopia among Chinese suburban adolescents does not seem to be as strong as that of sleep habits. Future research could seek to provide consistent evidence for a potential association between myopia and sleep habits.
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http://dx.doi.org/10.1155/2020/2670153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399760PMC
July 2020

OSBPL2 Is Required for the Binding of COPB1 to ATGL and the Regulation of Lipid Droplet Lipolysis.

iScience 2020 Jul 20;23(7):101252. Epub 2020 Jun 20.

Department of Medical Genetics, School of Basic Medical Science, Nanjing Medical University, Nanjing 211166, China; Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing 211166, China; The Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China. Electronic address:

The accumulation of giant lipid droplets (LDs) increases the risk of metabolic disorders including obesity and insulin resistance. The lipolysis process involves the activation and transfer of lipase, but the molecular mechanism is not completely understood. The translocation of ATGL, a critical lipolysis lipase, from the ER to the LD surface is mediated by an energy catabolism complex. Oxysterol-binding protein-like 2 (OSBPL2/ORP2) is one of the lipid transfer proteins that regulates intracellular cholesterol homeostasis. A recent study has proven that Osbpl2 pigs exhibit hypercholesterolemia and obesity phenotypes with an increase in adipocytes. In this study, we identified that OSBPL2 links the endoplasmic reticulum (ER) with LDs, binds to COPB1, and mediates ATGL transport. We provide important insights into the function of OSBPL2, indicating that it is required for the regulation of lipid droplet lipolysis.
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http://dx.doi.org/10.1016/j.isci.2020.101252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348002PMC
July 2020

Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells.

Int J Biol Macromol 2020 Oct 15;161:797-809. Epub 2020 Jun 15.

School of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan, Province, Kunming 650500, China. Electronic address:

This study isolated and characterized a novel polysaccharide (PNPS-0.3) from the residue of Panax notoginseng by gradient elution. PNPS-0.3 mainly consisted of a backbone of →4)- α-D-GalAp-(1 → 4-β-L-Rhap-1 → 4)-β-D-Galp-(1 → residues, with an α-L-Araf-1 → 5)-α-L-Araf-(1 → branch connecting to the backbone at O-3 of →4-β-L-Rhap-1 → and a molecular weight of 76,655 Da. Furthermore, the adjuvant potential of PNPS-0.3 with bone marrow dendritic cells (BMDCs) was investigated. The results suggested that PNPS-0.3 could induce maturation of BMDCs by reshaping the morphology, upregulating the CD40, CD80, CD86 and MHC II membrane phenotypic markers, and by promoting the secretion of TNF-α and IL-12 proinflammatory cytokines. Moreover, PNPS-0.3 can trigger the DC-induced T-cell immune response, as indicated by the higher expressions of CD4, CD8, CD69, and MHC II in T cells with increased secretion of INF-β. Furthermore, PNPS-0.3 can bind to the pattern recognition receptors (PRR) of Toll-like receptor 4 (TLR 4), Toll-like receptor 2 (TLR 2), and mannose receptor (MR) on BMDCs. PNPS-0.3 also upregulated the expressions of Myd88, IKKβ, PP65, T-P65, and NF-κB, suggesting that the TLR4/TLR2-NF-κB signaling pathway was involved in the immunomodulatory mechanism. In conclusion, the immunoadjuvant potential of novel PNPS-0.3 was characterized, which is beneficial for the future utilization and development of P. notoginseng.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.117DOI Listing
October 2020

Correction: Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.

Cell Res 2020 Jul;30(7):630

CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41422-020-0352-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414023PMC
July 2020

Hydrophobic deep eutectic solvents based membrane emulsification-assisted liquid-phase microextraction method for determination of pyrethroids in tea beverages.

J Chromatogr A 2020 Jul 12;1623:461204. Epub 2020 May 12.

Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China. Electronic address:

Deep eutectic solvents (DESs) were used as an extractant for the determination of pyrethroids in environmental water and tea beverage samples. Three different acids were chosen as hydrogen bond donors for preparation of DESs, and decanoic acid was optimal because of its high recovery. Factors affecting relative recovery were optimized individually, including salt addition, surfactant addition, extraction temperature, DES amount, and sample volume etc. Under the optimized experimental conditions, the relative recovery of pyrethroids was from 89.3% to 97.7%, with relative standard deviation (RSD) values ranging from 1.75% to 2.73%. The linear correlation coefficient ranged from 0.9981 to 0.9992, and the linear range was between 1.9 and 500 μg/L. Based on a signal-to-noise ratio (S/N) of 3:1, the limit of detection (LOD) values were 0.56 to 1.24 μg/L. The enrichment factor ranged from 92 to 105. In conclusion, good extraction efficiency was achieved in tea beverage samples under the optimized conditions.
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http://dx.doi.org/10.1016/j.chroma.2020.461204DOI Listing
July 2020

Guanxin V for coronary artery disease: A retrospective study.

Biomed Pharmacother 2020 Aug 30;128:110280. Epub 2020 May 30.

Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, China. Electronic address:

Background: Guanxin V (GXV), a traditional herbal mixture, has been widely used in clinical practice for the treatment of coronary artery disease (CAD). This retrospective study was designed to assess the safety and effectiveness of GXV for CAD.

Methods: In our study, December 2006 to January 2009, 101 patients with CAD from Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine were enrolled, of whom 52 patients received GXV plus guideline-recommended medical therapy (GMT) (GXV group), 49 patients received GMT alone (GMT group). The general clinical information, traditional Chinese medicine syndrome score (TSS), the therapeutic effects, 6-minute walk test (6MWT), adverse events, echocardiography, and laboratory information were collected and analyzed pre-and post-treatment.

Results: We did not find differences in the information between the two groups before treatment. Patients in the GXV group had decreased TSS (P < 0.0001) and increased therapeutic effects (P = 0.763) and 6MWT (P < 0.0001) than those in the GMT group and there were no significant differences in safety between the two groups. Moreover, patients in the GXV group improved ejection fraction, cardiac output, and stroke volume (P = 0.2113, 0.0001, 0.0002, respectively), and dropped BNP (P = 0.3856) compared with those in the GMT group.

Conclusions: Superiority in the GXV group for patients with CAD was demonstrated over the GMT group for both the safety and effectiveness endpoints. This suggests that GXV is a potentially safe and effective treatment for CAD patients.
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http://dx.doi.org/10.1016/j.biopha.2020.110280DOI Listing
August 2020

Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.

Cell Res 2020 07 4;30(7):610-622. Epub 2020 May 4.

CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.

Innate lymphoid cells (ILCs) reside in mucosal surfaces to potentiate immune responses, sustain mucosal integrity and maintain tissue homeostasis. However, how tumor infiltrating ILCs modulate tumor development and progression is unclear. Here we profiled tumor infiltrating ILCs during colorectal cancer (CRC) progression by single-cell RNA sequencing. We identified six clusters of tumor infiltrating ILCs with unique features. ILC1s expressed inhibitory receptors and underwent inhibitory functional conversion at the late stage of CRC. ILC2s were classified into three subsets (called ILC2-A, -B, -C), of which ILC2-C subset could facilitate tumor progression. HS3ST1 and PD1 were highly expressed in ILC2s of late stage CRC tumors and deficiency of HS3ST1 or PD1 in ILC2s suppressed tumor growth. Moreover, ILC3s transdifferentiated into ILCregs during CRC progression and ILCregs promoted tumor growth. Of note, TGF-β signaling initiated the conversion of ILC3s to ILCregs and blockade of TGF-β signaling could disrupt the ILCreg transdifferentiation and inhibited tumor growth. Thus, intervention of ILC conversions might be a potential strategy for CRC immunotherapy.
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http://dx.doi.org/10.1038/s41422-020-0312-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343789PMC
July 2020
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