Publications by authors named "Yuan Lu"

733 Publications

The Emerging Roles of Long Noncoding RNAs as Hallmarks of Lung Cancer.

Front Oncol 2021 7;11:761582. Epub 2021 Oct 7.

State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.

Noncoding ribonucleic acids (ncRNAs) are closely associated with tumor initiation, growth, and progress in lung cancer. Long ncRNAs (lncRNAs), as one of the three subclasses of ncRNAs, play important roles in chromatin modification, transcription, and post-transcriptional processing. Various lncRNAs have recently been reported to be dysfunctional or dysregulated in cancers and have pro- or anti-tumor potential. Importantly, as a new class of cancer biomarkers, studies have demonstrated the plausibility of using certain subsets of lncRNAs as promising diagnostic, therapeutic, or prognostic strategies to manage cancers. This review focuses on lncRNAs associated with hallmarks of lung cancer, especially those discovered in the last five years. The expression levels of these lncRNAs in tumor samples are discussed, alongside their mechanisms of action, drug resistance, and potential as diagnostic and prognostic markers for lung cancer.
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http://dx.doi.org/10.3389/fonc.2021.761582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529012PMC
October 2021

Genome-Wide Regulation of Acupuncture and Moxibustion on Ulcerative Colitis Rats.

Evid Based Complement Alternat Med 2021 8;2021:9945121. Epub 2021 Oct 8.

Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Acupuncture and moxibustion have definite clinical effects on treating ulcerative colitis (UC), but their mechanism is still unclear. To investigate the molecular mechanisms, we applied herb-partitioned moxibustion or electroacupuncture at the Tianshu (ST25) points on UC rats and used RNA sequencing to identify molecular consequences. Male Sprague Dawley (SD) rats were divided into 6 groups randomly: the normal control (NC) group, the control + herb-partitioned moxibustion (NCHM) group, the control + electroacupuncture (NCEA) group, the model (UC) group, the model + herb-partitioned moxibustion (UCHM) group, and the model + electroacupuncture (UCEA) group. Compared to the UC group, HE staining in the UCHM group and UCEA group indicated that colitis was relieved, the histopathological score and MPO were both significantly reduced, and the serum hs-CRP concentration was decreased significantly. The results of RNA-seq suggested that, compared to the NC group, 206 upregulated genes and 167 downregulated genes were identified in colon tissues from the UC group; compared to the UC group, the expression levels of some genes were both affected in the UCHM group and the UCEA group (684 differentially expressed genes were identified in the UCHM group, and 1182 differentially expressed genes were identified in the UCEA group). KEGG signal pathway analysis indicated that the differentially expressed genes in the UCHM group were associated with the JAK-STAT signaling pathway and cell adhesion molecule (CAM); the differentially expressed genes in the UCEA group were associated with the NF-B signaling pathway, the toll-like receptor signaling pathways, the PI3K-Akt signaling pathway, the MAPK signaling pathway, and the Wnt signaling pathway. This is the first study to reveal the gene expression characteristics of the anti-inflammatory effect of UC rats from the perspective of acupuncture and moxibustion control, which provide a clue for further investigation into the molecular mechanisms of UC treatment by acupuncture and moxibustion.
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http://dx.doi.org/10.1155/2021/9945121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519700PMC
October 2021

Persistent Hypertension: A Broader Framework for Improving Blood Pressure Control.

Am J Med 2021 Oct 14. Epub 2021 Oct 14.

Center for Outcomes Research and Evaluation, Yale New Haven Hospital, New Haven, CT; Section of Cardiovascular Medicine, Department of Internal Medicine, Yale School of Medicine, New Haven, CT; Department of Health Policy and Management, Yale School of Public Health, New Haven, CT. Electronic address:

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http://dx.doi.org/10.1016/j.amjmed.2021.09.018DOI Listing
October 2021

The complete chloroplast genome of C. B. Clarke (Cyperaceae), an endemic species from China.

Mitochondrial DNA B Resour 2021 5;6(11):3117-3118. Epub 2021 Oct 5.

Xi'an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi'an, Shaanxi, China.

C. B. Clarke is a sedge with excellent ornamental characters, it is an important ecosystem stabilizer. Here we report the complete chloroplast genome of to provide a foundation for further phylogenetic studies on the Cyperaceae. The chloroplast (cp) genome is 184,157 bp in size and consists of a large single-copy (LSC) region 106,654 bp in length, a small single-copy (SSC) region of 36,099 bp, two inverted repeats (IR) regions each 20,702 bp. The total GC content of the cp genome is 33.9% with the LSC, SSC, and IR regions 32, 32.5, and 42.9%, respectively. The cp genome contains 128 genes, including 80 protein-coding, 40 tRNA, and eight rRNA genes. The phylogenetic analysis showed is in a clade with Maxim and Hance. This study provides a basis for further phylogenetic studies of Carex.
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http://dx.doi.org/10.1080/23802359.2021.1984326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510598PMC
October 2021

Mechanism of neutrophil extracellular traps generation and their role in trophoblasts apoptosis in gestational diabetes mellitus.

Cell Signal 2021 Oct 8;88:110168. Epub 2021 Oct 8.

Department of Obstetrics and Gynaecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, 324 Jingwu Street, Jinan, Shandong 250021, China; Department of Obstetrics and Gynaecology, Maternal and Child Health Care Hospital of Shandong Provincial, Cheeloo College of Medicine, Shandong University, 238 Jingshi East Road, Jinan, Shandong 250014, China; The Laboratory of Placenta-Related Diseases, Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, 238 Jingshi East Road, Jinan, Shandong 250014, China. Electronic address:

Gestational diabetes mellitus (GDM) is a metabolic syndrome occurring in pregnant women and increases the risk of placental dysplasia. Neutrophil extracellular traps (NETs) may play a critical role in placental dysplasia. NETosis (neutrophil cell death by NET release) depends on NADPH/ROS pathway. In view of the adiponectin which is widely believed to be reduced in GDM patients suppresses NADPH oxidase and ROS generation of neutrophil. We speculate that increased NET release is associated with hypoadiponectinemia. Trophoblast apoptosis is significantly increased in GDM patients, but it is not clear whether NETs promotes cell apoptosis. This study aims to reveal the mechanism of Neutrophil Extracellular Traps generation and their role in trophoblast apoptosis in Gestational Diabetes Mellitus. We investigated the generation of NETs by cell-free DNA (cf-DNA) quantification, live-cell imaging, and reactive oxygen species (ROS) measurement. ERK1/2 and p38 MAPK signalling pathway proteins were detected by western blotting. The Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and western blotting were performed to explore the effects of NETs on trophoblast apoptosis. We found that adiponectin inhibited NET release by suppressing ROS production, and p38 MAPK and ERK1/2 proteins were involved in the process. Further, NETs promoted trophoblast apoptosis by activating the ROS-dependent mitochondrial pathway, which is mediated by ERK1/2 signalling. The current study demonstrated that hypoadiponectinemia is the cause of NETs formation and NETs promoting trophoblast apoptosis.
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http://dx.doi.org/10.1016/j.cellsig.2021.110168DOI Listing
October 2021

Changes in ST segment elevation myocardial infarzzction hospitalisations in China from 2011 to 2015.

Open Heart 2021 09;8(2)

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, Beijing, People's Republic of China

Objective: Access to acute cardiovascular care has improved and health services capacity has increased over the past decades. We assessed national changes in (1) patient characteristics, (2) in-hospital management and (3) patient outcomes among patients presenting with ST segment elevation myocardial infarction (STEMI) in 2011-2015 in China.

Methods: In a nationally representative sample of hospitals in China, we created two random cohorts of patients in 2011 and 2015 separately. We weighted our findings to estimate nationally representative numbers and assessed changes from 2011 to 2015. Data were abstracted from medical charts centrally using standardised definitions.

Results: While the proportion of patients with STEMI among all patients with acute myocardial infarction decreased over time from 82.5% (95% CI 81.7 to 83.3) in 2011 to 68.5% (95% CI 67.7 to 69.3) in 2015 (p<0.0001), the weighted national estimate of patients with STEMI increased from 210 000 to 380 000. The rate of reperfusion eligibility among patients with STEMI decreased from 49.3% (95% CI 48.1 to 50.5) to 42.2% (95% CI 41.1 to 43.4) in 2015 (p<0.0001); ineligibility was principally driven by larger proportions with prehospital delay exceeding 12 hours (67.4%-76.7%, p<0.0001). Among eligible patients, the proportion receiving reperfusion therapies increased from 54% (95% CI 52.3 to 55.7) to 59.7% (95% CI 57.9 to 61.4) (p<0.0001). Crude and risk-adjusted rates of in-hospital death did not differ significantly between 2011 and 2015.

Conclusions: In this most recent nationally representative study of STEMI in China, the use of acute reperfusion increased, but no significant improvement occurred in outcomes. There is a need to continue efforts to prevent cardiovascular diseases, to monitor changes in in-hospital treatments and outcomes, and to reduce prehospital delay.
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http://dx.doi.org/10.1136/openhrt-2021-001666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488733PMC
September 2021

Comparison of Detection Methods of Microplastics in Landfill Mineralized Refuse and Selection of Degradation Degree Indexes.

Environ Sci Technol 2021 Oct 29;55(20):13802-13811. Epub 2021 Sep 29.

MOE Key Laboratory of Pollution Processes and Environmental Criteria/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.

A landfill is an important sink of plastic waste and potential sources of microplastics (MPs) when mineralized refuse is reused. However, limitations are still present in quantifying MPs in mineralized refuse and assessing their degradation degree. In this study, laser direct infrared spectroscopy and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify MPs of mineralized refuse from a landfill. Although 25-113 items/g MPs were detected in particles subjected to flotation, 37.9-674 μg/g polyethylene terephthalate (PET) and 0.0716-1.01 μg/g polycarbonate (PC) were detected in the residual solids by LC-MS/MS, indicating a great amount of plastic polymers still presented in the residue. This suggests that the commonly used flotation-counting method will lead to significant underestimation of MP pollution in mineralized refuse, which might be due to the aging and aggregation process caused by the long-term landfill process. The ratio of "bisphenol A/PC" and "plasticizer/MPs" was found to be positively correlated and negatively correlated with the landfill age, respectively. Therefore, in addition to the spectral index such as the carbonyl index, new indexes based on the concentrations of polymers, free monomers, and plasticizers were proposed to characterize the degradation degree of MPs in a landfill.
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http://dx.doi.org/10.1021/acs.est.1c02772DOI Listing
October 2021

Prevalence of Dyslipidemia and Availability of Lipid-Lowering Medications Among Primary Health Care Settings in China.

JAMA Netw Open 2021 Sep 1;4(9):e2127573. Epub 2021 Sep 1.

National Clinical Research Center for Cardiovascular Diseases, National Health Commission Key Laboratory of Clinical Research for Cardiovascular Medications, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Center for Cardiovascular Diseases, Beijing, China.

Importance: Dyslipidemia, the prevalence of which historically has been low in China, is emerging as the second leading yet often unaddressed factor associated with the risk of cardiovascular diseases. However, recent national data on the prevalence, treatment, and control of dyslipidemia are lacking.

Objective: To assess the prevalence, treatment, and control of dyslipidemia in community residents and the availability of lipid-lowering medications in primary care institutions in China.

Design, Setting, And Participants: This cross-sectional study used data from the China-PEACE (Patient-Centered Evaluative Assessment of Cardiac Events) Million Persons Project, which enrolled 2 660 666 community residents aged 35 to 75 years from all 31 provinces in China between December 2014 and May 2019, and the China-PEACE primary health care survey of 3041 primary care institutions. Data analysis was performed from June 2019 to March 2021.

Exposures: Study period.

Main Outcomes And Measures: The main outcome was the prevalence of dyslipidemia, which was defined as total cholesterol greater than or equal to 240 mg/dL, low-density lipoprotein cholesterol (LDL-C) greater than or equal to 160 mg/dL, high-density lipoprotein cholesterol (HDL-C) less than 40 mg/dL, triglycerides greater than or equal to 200 mg/dL, or self-reported use of lipid-lowering medications, in accordance with the 2016 Chinese Adult Dyslipidemia Prevention Guideline.

Results: This study included 2 314 538 participants with lipid measurements (1 389 322 women [60.0%]; mean [SD] age, 55.8 [9.8] years). Among them, 781 865 participants (33.8%) had dyslipidemia. Of 71 785 participants (3.2%) who had established atherosclerotic cardiovascular disease (ASCVD) and were recommended by guidelines for lipid-lowering medications regardless of LDL-C levels, 10 120 (14.1%) were treated. The overall control rate of LDL-C (≤70 mg/dL) among adults with established ASCVD was 26.6% (19 087 participants), with the control rate being 44.8% (4535 participants) among those who were treated and 23.6% (14 552 participants) among those not treated. Of 236 579 participants (10.2%) with high risk of ASCVD, 101 474 (42.9%) achieved LDL-C less than or equal to 100 mg/dL. Among participants with established ASCVD, advanced age (age 65-75 years, odds ratio [OR], 0.63; 95% CI, 0.56-0.70), female sex (OR, 0.56; 95% CI, 0.53-0.58), lower income (reference category), smoking (OR, 0.89; 95% CI, 0.85-0.94), alcohol consumption (OR, 0.87; 95% CI, 0.83-0.92), and not having diabetes (reference category) were associated with lower control of LDL-C. Among participants with high risk of ASCVD, younger age (reference category) and female sex (OR, 0.58; 95% CI, 0.56-0.59) were associated with lower control of LDL-C. Of 3041 primary care institutions surveyed, 1512 (49.7%) stocked statin and 584 (19.2%) stocked nonstatin lipid-lowering drugs. Village clinics in rural areas had the lowest statin availability.

Conclusions And Relevance: These findings suggest that dyslipidemia has become a major public health problem in China and is often inadequately treated and uncontrolled. Statins were available in less than one-half of the primary care institutions. Strategies aimed at detection, prevention, and treatment are needed.
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http://dx.doi.org/10.1001/jamanetworkopen.2021.27573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482054PMC
September 2021

[Pharmacokinetic study of Polygonum orientale extract in H9c2 cells by UPLC-MS/MS].

Zhongguo Zhong Yao Za Zhi 2021 Sep;46(18):4833-4840

Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University Guiyang 550004, China.

A detection method of ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was established to detect concentrations of isoorientin, orientin, quercetin, vitexin and kaempferol-3-O-β-D-glucoside in H9 c2 cells and applied to the pharmacokinetic study of Polygonum orientale extract in the cells. H9 c2 cells were treated with 100 μg·mL~(-1) P. orientale extract and then they and the corresponding nuclei, mitochondria and Golgi bodies were collected at the set time. After protein precipitation, UPLC-MS/MS was used to determine concentrations of isoorientin, orientin, quercetin, vitexin and kaempferol-3-O-β-D-glucoside in the whole cells and subcellular structures. Also, related pharmacokinetic parameters were calculated. The results showed that the peak time was 8 h for all these components. Orientin, vitexin, quercetin and isoorientin have high affinities to nuclei and mitochondria, while the affinity of kaempferol-3-O-β-D-glucoside is higher with mitochondria compared to nuclei. It is suggested that these chemical components of P. orientale may mainly act on nuclei or mitochondria to exert pharmacological effects of protecting cardiomyocytes.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210618.202DOI Listing
September 2021

Effectiveness of an impedance cardiography guided treatment strategy to improve blood pressure control in a real-world setting: results from a pragmatic clinical trial.

Open Heart 2021 09;8(2)

Department of Cardiology and Hypertension, Peking University People's Hospital, Beijing, China

Objective: To test the effectiveness of an impedance cardiography (ICG) guided treatment strategy on improving blood pressure (BP) control in real-world clinical practice.

Design: A single-centre, pragmatic randomised trial.

Setting: A hypertension clinic of the Peking University People's Hospital in Beijing, China.

Participants: Adults who sought outpatient care for hypertension in the hypertension clinic at the Peking University People's Hospital between June and December 2019.

Interventions: A computerised clinical decision support of recommending treatment choices to providers based on patients' haemodynamic profiles measured by ICG.

Main Outcome Measures: Changes in systolic BP (SBP) and diastolic BP (DBP) levels at the follow-up visit 4-12 weeks after baseline. Secondary outcomes included achievement of BP goal of <140/90 mm Hg and the changes in BP by baseline BP, age, sex and body mass index (BMI).

Results: A total of 102 adults (mean age was 54±14 years; 41% were women) completed the study. The mean baseline SBP was 150.9 (SD of 11.5) mm Hg and mean baseline DBP was 91.1 (11.3) mm Hg. At the follow-up visit, the mean SBP and DBP decreased by 19.9 and 11.3 mm Hg in the haemodynamic group, as compared with 12.0 and 4.9 mm Hg in the standard care group (p value for difference between groups <0.001). The proportion of patients achieving BP goal of <140/90 mm Hg in the haemodynamic group was 67%, as compared with 41% in the standard care group (p=0.017). The haemodynamic group had a larger effect on BP reduction consistently across subgroups by age, sex, BMI and baseline BP.

Conclusions: An ICG-guided treatment strategy led to greater reductions in BP levels than were observed with standard care in a real-world population of outpatients with hypertension. There is a need for further validation of this strategy for improving blood pressure treatment selection.

Trial Registration Number: NCT04715698.
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http://dx.doi.org/10.1136/openhrt-2021-001719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477318PMC
September 2021

Tracking Self-reported Symptoms and Medical Conditions on Social Media During the COVID-19 Pandemic: Infodemiological Study.

JMIR Public Health Surveill 2021 09 28;7(9):e29413. Epub 2021 Sep 28.

Center for Outcomes Research and Evaluation, Yale New Haven Hospital, New Haven, CT, United States.

Background: Harnessing health-related data posted on social media in real time can offer insights into how the pandemic impacts the mental health and general well-being of individuals and populations over time.

Objective: This study aimed to obtain information on symptoms and medical conditions self-reported by non-Twitter social media users during the COVID-19 pandemic, to determine how discussion of these symptoms and medical conditions changed over time, and to identify correlations between frequency of the top 5 commonly mentioned symptoms post and daily COVID-19 statistics (new cases, new deaths, new active cases, and new recovered cases) in the United States.

Methods: We used natural language processing (NLP) algorithms to identify symptom- and medical condition-related topics being discussed on social media between June 14 and December 13, 2020. The sample posts were geotagged by NetBase, a third-party data provider. We calculated the positive predictive value and sensitivity to validate the classification of posts. We also assessed the frequency of health-related discussions on social media over time during the study period, and used Pearson correlation coefficients to identify statistically significant correlations between the frequency of the 5 most commonly mentioned symptoms and fluctuation of daily US COVID-19 statistics.

Results: Within a total of 9,807,813 posts (nearly 70% were sourced from the United States), we identified a discussion of 120 symptom-related topics and 1542 medical condition-related topics. Our classification of the health-related posts had a positive predictive value of over 80% and an average classification rate of 92% sensitivity. The 5 most commonly mentioned symptoms on social media during the study period were anxiety (in 201,303 posts or 12.2% of the total posts mentioning symptoms), generalized pain (189,673, 11.5%), weight loss (95,793, 5.8%), fatigue (91,252, 5.5%), and coughing (86,235, 5.2%). The 5 most discussed medical conditions were COVID-19 (in 5,420,276 posts or 66.4% of the total posts mentioning medical conditions), unspecified infectious disease (469,356, 5.8%), influenza (270,166, 3.3%), unspecified disorders of the central nervous system (253,407, 3.1%), and depression (151,752, 1.9%). Changes in posts in the frequency of anxiety, generalized pain, and weight loss were significant but negatively correlated with daily new COVID-19 cases in the United States (r=-0.49, r=-0.46, and r=-0.39, respectively; P<.05). Posts on the frequency of anxiety, generalized pain, weight loss, fatigue, and the changes in fatigue positively and significantly correlated with daily changes in both new deaths and new active cases in the United States (r ranged=0.39-0.48; P<.05).

Conclusions: COVID-19 and symptoms of anxiety were the 2 most commonly discussed health-related topics on social media from June 14 to December 13, 2020. Real-time monitoring of social media posts on symptoms and medical conditions may help assess the population's mental health status and enhance public health surveillance for infectious disease.
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http://dx.doi.org/10.2196/29413DOI Listing
September 2021

Pharmacokinetic herb-drug interactions between Aidi injection and doxorubicin in rats with diethylnitrosamine-induced hepatocellular carcinoma.

BMC Pharmacol Toxicol 2021 09 6;22(1):48. Epub 2021 Sep 6.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, 550004, China.

Background: Aidi Injection (ADI), a Chinese herbal preparation with anti-cancer activity, is used for the treatment of hepatocellular carcinoma (HCC). Several clinical studies have shown that co-administration of ADI with doxorubicin (DOX) is associated with reduced toxicity of chemotherapy, enhanced clinical efficacy and improved quality of life for patients. However, limited information is available about the herb-drug interactions between ADI and DOX. The study aimed to investigate the pharmacokinetic mechanism of herb-drug interactions between ADI and DOX in a rat model of HCC.

Methods: Experimental HCC was induced in rats by oral administration of diethylnitrosamine. The HCC rats were pretreated with ADI (10 mL/kg, intraperitoneal injection) for 14 consecutive days prior to administration of DOX (7 mg/kg, intravenous injection) to investigate pharmacokinetic interactions. Plasma concentrations of DOX and its major metabolite, doxorubicinol (DOXol), were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

Results: Preadministration of ADI significantly altered the pharmacokinetics of DOX in HCC rats, leading to increased plasma concentrations of both DOX and DOXol. The area under the plasma drug concentration-time curve (AUCs) of DOX and DOXol in rats pretreated with ADI were 3.79-fold and 2.92-fold higher, respectively, than those in control rats that did not receive ADI.

Conclusions: Increased levels of DOX and DOXol were found in the plasma of HCC rats pretreated with ADI.
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http://dx.doi.org/10.1186/s40360-021-00515-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419969PMC
September 2021

Photoassisted Electron-Ion Synergic Doping Induced Phase Transition of n-VO/p-GaN Thin-Film Heterojunction.

ACS Appl Mater Interfaces 2021 Sep 1;13(36):43562-43572. Epub 2021 Sep 1.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, P. R. China.

As a typical correlated metal oxide, vanadium dioxide (VO) shows specific metal-insulator transition (MIT) properties and demonstrates great potential applications in ultrafast optoelectronic switch, resistive memory, and neuromorphic devices. Effective control of the MIT process is essential for improving the device performance. In the current study, we have first proposed a photoassisted ion-doping method to modulate the phase transition of the VO layer based on the photovoltaic effect and electron-ion synergic doping in acid solution. Experimental results show that, for the prepared n-VO/p-GaN nanojunction, this photoassisted strategy can effectively dope the n-VO layer by H, Al, or Mg ions under light radiation and trigger consecutive insulator-metal-insulator transitions. If combined with standard lithography or electron beam etching processes, selective doping with nanoscale size area can also be achieved. This photoassisted doping method not only shows a facile route for MIT modulation via a doping route under ambient conditions but also supplies some clues for photosensitive detection in the future.
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http://dx.doi.org/10.1021/acsami.1c10401DOI Listing
September 2021

Malic enzyme 2 promotes the progression of hepatocellular carcinoma via increasing triglyceride production.

Cancer Med 2021 Oct 24;10(19):6795-6806. Epub 2021 Aug 24.

Department of Interventional Radiology, The Affiliated Cancer Hospital of Guizhou Medical University, Guiyang, China.

The incidence and mortality of hepatocellular carcinoma (HCC) are gradually increasing during the past years. Recently, some studies have reported that malic enzyme (ME) plays an important role in cancer development, while the involvement of ME2 in HCC remains still undetermined. Here, we demonstrated that ME2 played an oncogenic role in HCC. ME2 was overexpressed in HCC tissues. TCGA database showed that the ME2 transcript level was inversely associated with the survival of HCC patients. Loss-of-function and gain-of-function assays showed that ME2 promoted HCC cell growth and migration. Furthermore, the xenografted tumorigenesis of MHCC97H cells was retarded by ME2 knockdown. ME2 silencing also suppressed the cell cycle process and induced apoptosis. Mechanistically, ME2 potentiated triglyceride synthesis, inhibition of which suppressed the proliferation and migration. We propose that ME2 promotes HCC progression by increasing triglyceride production.
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http://dx.doi.org/10.1002/cam4.4209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495273PMC
October 2021

Trends in Differences in Health Status and Health Care Access and Affordability by Race and Ethnicity in the United States, 1999-2018.

JAMA 2021 Aug;326(7):637-648

Center for Outcomes Research and Evaluation, Yale New Haven Hospital, New Haven, Connecticut.

Importance: The elimination of racial and ethnic differences in health status and health care access is a US goal, but it is unclear whether the country has made progress over the last 2 decades.

Objective: To determine 20-year trends in the racial and ethnic differences in self-reported measures of health status and health care access and affordability among adults in the US.

Design, Setting, And Participants: Serial cross-sectional study of National Health Interview Survey data, 1999-2018, that included 596 355 adults.

Exposures: Self-reported race, ethnicity, and income level.

Main Outcomes And Measures: Rates and racial and ethnic differences in self-reported health status and health care access and affordability.

Results: The study included 596 355 adults (mean [SE] age, 46.2 [0.07] years, 51.8% [SE, 0.10] women), of whom 4.7% were Asian, 11.8% were Black, 13.8% were Latino/Hispanic, and 69.7% were White. The estimated percentages of people with low income were 28.2%, 46.1%, 51.5%, and 23.9% among Asian, Black, Latino/Hispanic, and White individuals, respectively. Black individuals with low income had the highest estimated prevalence of poor or fair health status (29.1% [95% CI, 26.5%-31.7%] in 1999 and 24.9% [95% CI, 21.8%-28.3%] in 2018), while White individuals with middle and high income had the lowest (6.4% [95% CI, 5.9%-6.8%] in 1999 and 6.3% [95% CI, 5.8%-6.7%] in 2018). Black individuals had a significantly higher estimated prevalence of poor or fair health status than White individuals in 1999, regardless of income strata (P < .001 for the overall and low-income groups; P = .03 for middle and high-income group). From 1999 to 2018, racial and ethnic gaps in poor or fair health status did not change significantly, with or without income stratification, except for a significant decrease in the difference between White and Black individuals with low income (-6.7 percentage points [95% CI, -11.3 to -2.0]; P = .005); the difference in 2018 was no longer statistically significant (P = .13). Black and White individuals had the highest levels of self-reported functional limitations, which increased significantly among all groups over time. There were significant reductions in the racial and ethnic differences in some self-reported measures of health care access, but not affordability, with and without income stratification.

Conclusions And Relevance: In a serial cross-sectional survey study of US adults from 1999 to 2018, racial and ethnic differences in self-reported health status, access, and affordability improved in some subgroups, but largely persisted.
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http://dx.doi.org/10.1001/jama.2021.9907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371573PMC
August 2021

A linear DNA template-based framework for site-specific unnatural amino acid incorporation.

Synth Syst Biotechnol 2021 Sep 31;6(3):192-199. Epub 2021 Jul 31.

Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Site-specific incorporation of unnatural amino acids (UNAAs) into proteins using an orthogonal translation system (OTS) has expanded the scope of protein-coding chemistry. The key factor affecting UNAA embedding efficiency is the orthogonality of the OTS. Compared to traditional cell systems, cell-free systems are more convenient to control the reaction process and improve the utilization rate of UNAA. In this study, a linear DNA template-based cell-free unnatural protein synthesis system for rapid high-throughput screening and evolution was proposed. A total of 14 cell extracts were selected for screening out cell extract with high expression level. The result showed that EcAR7 ΔA ΔSer cell extract was optimal for the cell-free system. In addition, the screening results of four UNAAs, p-propargyloxy-l-phenylalanine (pPaF), p-azyl-phenylalanine (pAzF), p-acetyl-l-phenylalanine (pAcF), and p-benzoyl-l-phenylalanine (pBpF), showed that o-aaRS and o-tRNA of pPaF had good orthogonality. A new pair of corresponding o-aaRS and o-tRNA for pBpF was screened out. These results proved that this method could speed up the screening of optimal OTS components for UNAAs with versatile functions.
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http://dx.doi.org/10.1016/j.synbio.2021.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347695PMC
September 2021

The Novel Interplay between Commensal Gut Bacteria and Metabolites in Diet-Induced Hyperlipidemic Rats Treated with Simvastatin.

J Proteome Res 2021 Aug 9. Epub 2021 Aug 9.

Department of Microbiology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China.

Hyperlipidemia is one kind of metabolic syndrome for which the treatment commonly includes simvastatin (SV). Individuals vary widely in statin responses, and growing evidence implicates gut microbiome involvement in this variability. However, the associated molecular mechanisms between metabolic improvement and microbiota composition following SV treatment are still not fully understood. In this study, combinatory approaches using ultrahigh-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF MS/MS)-based metabolomic profiling, PCR-denaturing gradient gel electrophoresis (PCR-DGGE), quantitative PCR (qPCR), and 16S rRNA gene sequencing-based gut microbiota profiling were performed to investigate the interplay of endogenous metabolites and the gut microbiota related to SV treatment. A total of 6 key differential endogenous metabolites were identified that affect the metabolism of amino acids (phenylalanine and tyrosine), unsaturated fatty acids (linoleic acid and 9-hydroxyoctadecadienoic acid (9-HODE)), and the functions of gut microbial metabolism. Moreover, a total of 22 differentially abundant taxa were obtained following SV treatment. Three bacterial taxa were identified to be involved in SV treatment, namely, , , and . These findings suggested that the phenylalanine and tyrosine-associated amino acid metabolism pathways, as well as the linoleic acid and 9-HODE-associated unsaturated fatty acid metabolism pathways, which are involved in gut flora interactions, might be potential therapeutic targets for improvement in SV hypolipidemic efficacy. The mass spectrometric data have been deposited to MassIVE (https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp). Username: MSV000087842_reviewer. Password: hardworkingzsr.
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http://dx.doi.org/10.1021/acs.jproteome.1c00252DOI Listing
August 2021

Older Adults' Choice of Patterns of Outdoor Physical Activity Duration: A Mixed Multinomial Logit Model.

Int J Environ Res Public Health 2021 08 3;18(15). Epub 2021 Aug 3.

Urban Planning and Transportation, Department of the Built Environment, Eindhoven University of Technology, P.O. Box 513, 5600MB Eindhoven, The Netherlands.

Outdoor physical activity duration is a key component of outdoor physical activity behavior of older adults, and therefore, an important determinant of their total physical activity levels. In order to develop a successful outdoor physical activity program, it is important to identify any heterogeneity in preferences for outdoor physical activity duration patterns among older adults. In addition, more insight is needed in the influence of environmental characteristics on duration choice for creating supportive neighborhood environments matching individuals' preferences. To this end, a mixed multinomial logit model is estimated based on one-week data collected among 336 respondents aged 60 or over in 2017 in Dalian, China. The present model formulation accounts for heterogeneity in individuals' preferences and allows for the analysis of substitution and complementary relationships between the different patterns of outdoor physical activity duration. Results indicate that older adults vary significantly in their preferences for each outdoor physical activity duration pattern. Moreover, short walking duration, short exercise duration and medium exercise duration are substitutes for medium walking duration while short walking duration and short exercise duration are complements for medium exercise duration in terms of individuals' outdoor physical activity duration preferences. In addition, we find that distance to the nearest park, footpath conditions and neighborhood aesthetics are associated with older adults' outdoor physical activity duration choice.
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http://dx.doi.org/10.3390/ijerph18158199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346097PMC
August 2021

Short and Long-Term Innovations on Dietary Behavior Assessment and Coaching: Present Efforts and Vision of the Pride and Prejudice Consortium.

Int J Environ Res Public Health 2021 07 25;18(15). Epub 2021 Jul 25.

Division of Human Nutrition and Health, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.

Overweight, obesity and cardiometabolic diseases are major global health concerns. Lifestyle factors, including diet, have been acknowledged to play a key role in the solution of these health risks. However, as shown by numerous studies, and in clinical practice, it is extremely challenging to quantify dietary behaviors as well as influencing them via dietary interventions. As shown by the limited success of 'one-size-fits-all' nutritional campaigns catered to an entire population or subpopulation, the need for more personalized coaching approaches is evident. New technology-based innovations provide opportunities to further improve the accuracy of dietary assessment and develop approaches to coach individuals towards healthier dietary behaviors. Pride & Prejudice (P&P) is a unique multi-disciplinary consortium consisting of researchers in life, nutrition, ICT, design, behavioral and social sciences from all four Dutch Universities of Technology. P&P focuses on the development and integration of innovative technological techniques such as artificial intelligence (AI), machine learning, conversational agents, behavior change theory and personalized coaching to improve current practices and establish lasting dietary behavior change.
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http://dx.doi.org/10.3390/ijerph18157877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345591PMC
July 2021

Improving the ameliorative effects of berberine and curcumin combination via dextran-coated bilosomes on non-alcohol fatty liver disease in mice.

J Nanobiotechnology 2021 Aug 4;19(1):230. Epub 2021 Aug 4.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China.

Background: The combination of berberine (BER) and curcumin (CUR) has been verified with ameliorative effects on non-alcohol fatty liver disease (NAFLD). However, discrepant bioavailability and biodistribution of BER and CUR remained an obstacle to achieve synergistic effects. Multilayer nanovesicles have great potential for the protection and oral delivery of drug combinations. Therein lies bile salts inserted liposomes, named as bilosomes, that possesses long residence time in the gastrointestinal tract (GIT) and permeability across the small intestine. Diethylaminoethyl dextran (DEAE-DEX) is generally used as an outside layer on the nanovesicles to increase the mucinous stability and promote oral absorption. Herein, we developed a DEAE-DEX-coated bilosome with BER and CUR encapsulated ([email protected]) for the treatment of NAFLD.

Results: [email protected] with 150 nm size exhibited enhanced permeation across mucus and Caco-2 monolayer. In vivo pharmacokinetics study demonstrated that [email protected] profoundly prolonged the circulation time and improved the oral absorption of both BER and CUR. Intriguingly, synchronized biodistribution of BER and CUR and highest biodistribution at liver was achieved by [email protected], which contributed to the optimal ameliorative effects on NAFLD. It was further verified to be mainly mediated by anti-oxidation and anti-inflammation related pathways CONCLUSION: DEAE-DEX coated bilosome displayed promoted oral absorption, prolonged circulation and synchronized biodistribution of BER and CUR, leading to improved ameliorative effects on NAFLD in mice, which provided a promising strategy for oral administration of drug combinations.
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http://dx.doi.org/10.1186/s12951-021-00979-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336351PMC
August 2021

Degradation of Bisphenol A by ozonation in a rotating packed bed: Modeling by response surface methodology and artificial neural network.

Chemosphere 2021 Jul 27;286(Pt 2):131702. Epub 2021 Jul 27.

Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

The ozonation process of Bisphenol A (BPA) in a rotating packed bed (RPB) was modeled by response surface methodology (RSM) and artificial neural network (ANN). Experiments were performed according to the Box-Behnken design, and the interactive effects of various parameters including ozone concentration, pH, rotation speed of RPB and liquid flow rate on BPA degradation efficiency were investigated. Ozone concentration and pH had the most significant interactive effects on BPA degradation efficiency while rotation speed of RPB had no significant interactive effects with other variables. A second order polynomial equation was obtained to predict BPA degradation efficiency. Also, a multi-layered feed-forward ANN model was constructed based on the data of RSM experiments. Six neurons in hidden layer had the highest correlation coefficient (R = 0.99158). A comparison between RSM and ANN models suggested that both can accurately predict BPA degradation efficiency (R = 0.99559). The highest BPA degradation efficiency (99.52 %) was achieved under the conditions of ozone concentration of 20 mg L, pH of 11, liquid flow rate of 10 L h and rotation speed of RPB of 800 rpm, which was well predicted by the RSM model (99.54 %) and the ANN model (99.82 %). However, the RSM model was slightly better than the ANN model owing to its higher determination coefficient (R = 0.9912, R = 0.9827) and lower mean square error (MSE = 0.0001684, MSE = 0.0003305).
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http://dx.doi.org/10.1016/j.chemosphere.2021.131702DOI Listing
July 2021

Fasting Glucose of 6.1 mmol/L as a Possible Optimal Target for Type 2 Diabetic Patients with Insulin Glargine: A Randomized Clinical Trial.

J Diabetes Res 2021 14;2021:5524313. Epub 2021 Jul 14.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

To observe whether different insulin glargine titration algorithms based on fasting blood glucose (FBG) levels lead to different glycaemic variations (GVs) in type 2 diabetes (T2D) patients, a prospective, randomized, single-centre, comparative, three-arm parallel-group, open-label, treat-to-target, 24-week study was performed. A total of 71 uncontrolled T2D patients were recruited and randomized 1 : 3 : 3 into Groups 1, 2, and 3 (insulin titration goals of FBG ≤ 5.6, ≤6.1, and ≤7.0) for this study. The initiated insulin glargine dose was recommended at 0.2 U/kg/day and was then titrated following the FBG target. Patients were subjected to two 3-day continuous glucose monitoring (CGM) at baseline and the endpoint, wherein the CGM data were analysed, and the study's primary endpoint was the difference in 24 hrs mean amplitude of glycaemic excursion (MAGE) among the three groups. We observed that patients in the three groups had similar MAGE levels at the endpoint; however, Group 2 achieved a significant decrease in the MAGE level from baseline to the endpoint as compared to Groups 1 and 3 (all < 0.05). We also observed that these patients had significant glycated haemoglobin A1c (HbA1c) value improvements as compared to the other two groups (all < 0.05). Therefore, choosing an FBG level of 6.1 mmol/L as an insulin titration target provided significant GVs and HbA1c value improvements in T2D patients. Moreover, our data indicated that an FBG of 6.1 mmol/L could possibly be an insulin glargine titration target in T2D patients.
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http://dx.doi.org/10.1155/2021/5524313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294995PMC
July 2021

Trajectories of Pain After Cardiac Surgery: Implications for Measurement, Reporting, and Individualized Treatment.

Circ Cardiovasc Qual Outcomes 2021 08 26;14(8):e007781. Epub 2021 Jul 26.

Center for Outcomes Research and Evaluation, Yale-New Haven Hospital, New Haven, CT (M.M., Y.L., E.S.S., H.M.K.).

Background: Postoperative pain after cardiac surgery is a significant problem, but studies often report pain value as an average of the study cohort, obscuring clinically meaningful differences in pain trajectories. We sought to characterize heterogeneity in postoperative pain experiences.

Methods: We enrolled patients undergoing a cardiac surgery at a tertiary care center between January 2019 and February 2020. Participants received an electronically-delivered questionnaire every 3 days for 30 days to assess incision site pain level. We evaluated the variability in pain trajectories over 30 days by the cohort-level mean with confidence band and latent classes identified by group-based trajectory model. Group-based trajectory model estimated the probability of belonging to a specific trajectory of pain.

Results: Of 92 patients enrolled, 75 provided ≥3 questionnaire responses. The cohort-level mean showed a gradual and consistent decline in the mean pain level, but the confidence bands covered most of the pain score range. The individual-level trajectories varied substantially across patients. Group-based trajectory model identified 4 pain trajectories: persistently low (n=9, 12%), moderate declining (initially mid-level, followed by decline; n=26, 35%), high declining (initially high-level, followed by decline; n=33, 44%), and persistently high pain (n=7, 9%). Persistently high pain and high declining groups did not seem to be clearly distinguishable until approximately postoperative day 10. Patients in persistently low pain trajectory class had a numerically lower median age than the other 3 classes and were below the lower confidence band of the cohort-level approach. Patients in the persistently high pain trajectory class had a longer median length of hospital stay than the other 3 classes and were often higher than the upper confidence band of the cohort-level approach.

Conclusions: We identified 4 trajectories of postoperative pain that were not evident from a cohort-level mean, which has been a common way of reporting pain level. This study provides key information about the patient experience and indicates the need to understand variation among sites and surgeons and to investigate determinants of different experience and interventions to mitigate persistently high pain.
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http://dx.doi.org/10.1161/CIRCOUTCOMES.120.007781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366534PMC
August 2021

3D Magnetic Field-Controlled Synthesis, Collective Motion, and Bioreaction Enhancement of Multifunctional Peasecod-like Nanochains.

ACS Appl Mater Interfaces 2021 Aug 23;13(30):36157-36170. Epub 2021 Jul 23.

Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Magnetic field-induced synthesis and biocatalysis of magnetic materials have inspired great interest due to the flexible controllability of morphologies and unique magnetoelectrical properties. However, the interaction of the magnetic field and the reaction kinetics during the synthesis of magnetic nanochains has not been revealed. The collective motions in fluids and the multifunctional enhancements for bioreaction of 3D magnetic-controlled nanochains have not been systematically researched. Here, an integrated 3D magnetic control method was reported for the synthesis, collective motion, and multifunctional bioreaction enhancement of peasecod-like nanochains. The interactions of magnetic field and reaction kinetics were rationally controlled to synthesize magnetic nanochains of different morphologies. Collective motions of nanochains under alternating magnetic fields were studied to provide insights into the disturbance on confined fluids. Three mechanisms of reaction enhancement of nanostir, magnetic agent, and nanocatalyst were achieved simultaneously via 3D magnetic-controlled nanochains using a glucose oxidase-horseradish peroxidase multi-enzyme system. The peasecod-like nanochain also exhibited excellent reaction enhancement in cell-free protein synthesis reaction, which is desired for effective high-throughput screening. The integrated 3D magnetic control method through the whole process from fabrication to applications of magnetic nanomaterials could be extended to multifunctional biocatalysis and multi-task biomedicine.
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http://dx.doi.org/10.1021/acsami.1c08130DOI Listing
August 2021

[Research progress on chemical constituents and pharmacological activities of iridoid glycosides in Lonicera japonica].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(11):2746-2752

College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250000, China.

As a traditional Chinese medicinal material, Lonicera japonica has a long medicinal history. The chemical constituents of Lonicera japonica are complex, mainly including iridoid glycosides, flavonoids, triterpenes, organic acids and volatile oil. Iridoid glycosides account for a higher proportion. In addition, modern pharmacological studies have shown that the iridoid glycosides have many pharmacological activities such as antivirus, anti-inflammation, anti-tumor, liver protection and lowering blood sugar. This review intends to systematically summarize the iridoid glycosides identified from Lonicera japonica and their pharmacological activities by searc-hing Chinese and English databases, in order to provide a reference for the further development and utilization of Lonicera japonica and for the improvement of quality standards of medicinal materials.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210108.602DOI Listing
June 2021

Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis.

Bioresour Bioprocess 2021 6;8(1):58. Epub 2021 Jul 6.

Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084 China.

Cell-free protein synthesis (CFPS) systems have become an ideal choice for pathway prototyping, protein production, and biosensing, due to their high controllability, tolerance, stability, and ability to produce proteins in a short time. At present, the widely used CFPS systems are mainly based on strain. , and are potential chassis cells for many biotechnological applications with their respective characteristics. Therefore, to expand the platform of the CFPS systems and options for protein production, four prokaryotes, , , and were selected as host organisms to construct the CFPS systems and be compared. Moreover, the process parameters of the CFPS system were optimized, including the codon usage, plasmid synthesis competent cell selection, plasmid concentration, ribosomal binding site (RBS), and CFPS system reagent components. By optimizing and comparing the main influencing factors of different CFPS systems, the systems can be optimized directly for the most influential factors to further improve the protein yield of the systems. In addition, to demonstrate the applicability of the CFPS systems, it was proved that the four CFPS systems all had the potential to produce therapeutic proteins, and they could produce the receptor-binding domain (RBD) protein of SARS-CoV-2 with functional activity. They not only could expand the potential options for in vitro protein production, but also could increase the application range of the system by expanding the cell-free protein synthesis platform.

Supplementary Information: The online version contains supplementary material available at 10.1186/s40643-021-00413-2.
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http://dx.doi.org/10.1186/s40643-021-00413-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258279PMC
July 2021

Creating a Social Learning Environment for and by Older Adults in the Use and Adoption of Smartphone Technology to Age in Place.

Front Public Health 2021 16;9:568822. Epub 2021 Jun 16.

Department of Industrial Design, Eindhoven University of Technology, Eindhoven, Netherlands.

Smartphone technologies can support older adults in their daily lives as they age in place at home. However, they may struggle to use these technologies which impacts acceptance, adoption, and sustainable use. Peer to peer community learning has the potential to support older adults to learn using (smartphone) technologies. This paper studies such a learning community approach and how it can support older adults to learn using and adopt the smartphone application GoLivePhone. This technology assists older adults in their daily living by supporting them through fall detection and activity tracking. In particular, the interface of this application can evolve and adapt as older adults become more knowledgeable during the use process or as their abilities change. This paper shows a field study with seven older adults learning and using the GoLivePhone technology through a living lab approach. These older adults participated in this research in a technology learning community that was set-up for research purposes. For this we used ordinary Samsung A3 smartphones with the simplified GoLivePhone software, particularly designed for older adults. At the end of the learning class we conducted an additional focus group to both explore factors facilitating older adults to learn using this technology and to identify their main personal drivers and motivators to start and adopt this technology. We collected qualitative data via open questions and audio recording during the focus group. This collected data was subject to a thematic analysis, coding was primarily performed by the first author, and reviewed by the other authors. We provide insights into how peer to peer community learning can contribute, and found both and recall tools to be helpful to support sustainable use of smartphone technology to support older adults to age in place.
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http://dx.doi.org/10.3389/fpubh.2021.568822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241932PMC
July 2021

Large Perpendicular Magnetic Anisotropy in Ta/CoFeB/MgO on Full-Coverage Monolayer MoS and First-Principles Study of Its Electronic Structure.

ACS Appl Mater Interfaces 2021 Jul 1;13(27):32579-32589. Epub 2021 Jul 1.

Institut Jean Lamour, Université de Lorraine, CNRS UMR7198, Campus ARTEM, 2 Allée André Guinier, BP 50840, 54011 Nancy, France.

A perpendicularly magnetized spin injector with a high Curie temperature is a prerequisite for developing spin optoelectronic devices on two-dimensional (2D) materials working at room temperature (RT) with zero applied magnetic field. Here, we report the growth of Ta/CoFeB/MgO structures with large perpendicular magnetic anisotropy (PMA) on full-coverage monolayer (ML) molybdenum disulfide (MoS). A large perpendicular interface anisotropy energy of 0.975 mJ/m has been obtained at the CoFeB/MgO interface, comparable to that observed in magnetic tunnel junction systems. It is found that the insertion of MgO between the ferromagnetic (FM) metal and the 2D material can effectively prevent the diffusion of the FM atoms into the 2D material. Moreover, the MoS ML favors a MgO(001) texture and plays a critical role in establishing the large PMA. First-principles calculations on a similar Fe/MgO/MoS structure reveal that the MgO thickness can modify the MoS band structure, from a direct band gap with 3ML-MgO to an indirect band gap with 7 ML-MgO. The proximity effect induced by Fe results in splitting of 10 meV in the valence band at the Γ point for the 3ML-MgO structure, while it is negligible for the 7 ML-MgO structure. These results pave the way to develop RT spin optoelectronic devices based on 2D transition-metal dichalcogenide materials.
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http://dx.doi.org/10.1021/acsami.1c08805DOI Listing
July 2021

Effect of hydroxychloroquine on antiphospholipid antibodies-inhibited endometrial angiogenesis.

J Matern Fetal Neonatal Med 2021 Jun 28:1-9. Epub 2021 Jun 28.

Department of Obstetrics and Gynaecology,Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Background: Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombotic events and/or pregnancy morbidity (≥3 recurrent early miscarriage or fetal death or a prematurity <34 weeks of gestation) with persistently positive antiphospholipid antibodies (aPLs). It is reported that aPLs damage the placental tissue by binding to β2-glycoprotein I (β2GPI) on the surface of trophoblast and endothelial cells. Hydroxychloroquine (HCQ) is considered to be beneficial in the treatment of obstetrical APS and shown to restore the aPL-inhibited invasion and differentiation of trophoblast. However, not enough evidence exists regarding the effect of HCQ on endometrial angiogenesis. The aim of our study was to assess whether HCQ has an effect on aPL-inhibited endothelial angiogenesis.

Methods: In this research, to explore the effect of HCQ for angiogenesis, we investigated: (1) Human umbilical vein endothelial cells (HUVECs) viability by CCK-8; (2) HUVECs migration by wound healing; (3) HUVEC angiogenesis by Matrigel assay ; (4) mRNA expression of and by real-time quantitative Polymerase Chain Reaction (RT-PCR); (5) protein expression of VEGF, MMP-2 by western blot.

Results: We found that HCQ treatment significantly restored the expression of aPL-inhibited and . HCQ restored aPL-inhibited HUVEC proliferation, migration, and angiogenesis .

Conclusion: In conclusion, aPLs inhibit HUVECs angiogenesis, however, HCQ can restore the effect of aPL-inhibited HUVECs migration and angiogenesis , demonstrating its beneficial therapeutic role in obstetrical APS.
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http://dx.doi.org/10.1080/14767058.2021.1943656DOI Listing
June 2021
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