Publications by authors named "Yuan Li"

4,836 Publications

  • Page 1 of 1

Bioequivalence and Pharmacokinetic Study of 2 Edoxaban Tablets in Healthy Chinese Subjects.

Clin Pharmacol Drug Dev 2022 Aug 11. Epub 2022 Aug 11.

Nanjing Clinical Tech Laboratories Inc., Nanjing, Jiangsu, China.

The aim was to investigate the bioequivalence of 2 orally administered edoxaban 60-mg tablets and the food effects on the pharmacokinetics of edoxaban. Sixty-four healthy Chinese subjects participated in this open-label, randomized, 2-sequence, 4-period, crossover study. All subjects randomly received 60-mg generic (test) or branded (reference) edoxaban tablet in each period. Plasma edoxaban concentrations were determined using liquid chromatography-tandem mass spectrometry. The pharmacokinetic parameters maximum concentration (C ) and area under the concentration-time curve (AUC) were compared to assess bioequivalence. The geometric least-squares mean ratios for C , AUC from time 0 to the last measurable time point (AUC ), and AUC from time 0 extrapolated to infinity (AUC ) were 97.0%, 95.4%, and 96.1%, respectively, in the fasting test, and 98.6%, 100.0%, and 99.8%, respectively, in the fed test. Food increased exposure and prolonged the time to maximum concentration of edoxaban. Both formulations displayed comparable safety profiles, with no serious adverse events reported. The 2 products of edoxaban tablets are bioequivalent and safe in healthy Chinese volunteers. Food may have a modest effect on the pharmacokinetic properties of edoxaban.
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http://dx.doi.org/10.1002/cpdd.1156DOI Listing
August 2022

Clomiphene citrate priming increases sensitivity during ovarian stimulation in poor ovarian responders undergoing fertilization treatment: a retrospective cohort study.

Hum Fertil (Camb) 2022 Aug 11:1-7. Epub 2022 Aug 11.

Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

This single-centre retrospective cohort study aimed to investigate whether a clomiphene citrate (CC) priming protocol could increase ovarian sensitivity in poor ovarian responders. It included 294 patients (374 ovarian stimulation cycles). Of these, 193 cycles were treated by a CC priming antagonist protocol (study group) and 181 by the classical flexible gonadotropin-releasing hormone antagonist protocol (control group). Stimulation data and laboratory and clinical outcomes were compared between the groups. The results showed that in the study group, total gonadotropin dosage and dosage per follicle were considerably lower, the follicle-to-oocyte index was significantly higher, and the gonadotropin duration was shorter. After adjusting for potential confounders, multivariate regression analysis showed that cumulative ongoing pregnancy remained comparable between the groups (adjusted odds ratio: 0.761, 95% confidence interval: 0.300-1.933,  = 0.566). Age, body mass index, gonadotropin dosage per follicle, and the follicle-to-oocyte index were negatively associated with the reproductive outcomes. The result of the sensitivity analysis showed that patients in the study group were administered less gonadotropin at a lower gonadotropin dosage per follicle and for a shorter duration. In conclusion, the CC priming antagonist protocol offered a convenient and patient-friendly way to increase ovarian sensitivity during ovarian stimulation in poor ovarian responders.
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http://dx.doi.org/10.1080/14647273.2022.2109069DOI Listing
August 2022

Application of dual-energy computed tomography in preoperative evaluation of Ki-67 expression levels in solid non-small cell lung cancer.

Medicine (Baltimore) 2022 Aug;101(31):e29444

GE Healthcare, Precision Health Institution, Shanghai, PR China.

To investigate whether there were significant differences in dual-energy CT (DECT) in reflecting different quantitative parameters among different levels of Ki-67 expression in patients with solid non-small cell lung cancer (NSCLC). The diagnosis performance of DECT in patients with solid lung adenocarcinoma (LAC) among NSCLC was further discusses. Two hundred fifteen patients confirmed with solid NSCLC were enrolled and analyzed retrospectively in this study. 148 patients were confirmed with LAC among all patients. Three expression levels of Ki-67 were determined by the percentage of Ki-67 positive cancer cells with immunohistochemistry: high-level group (>30%), middle-level group (10%-30%), and low-level group (≤10%). And the latter two levels also known as non-high-level group. The quantitative parameters of enhanced chest DECT (venous phase, VP), including iodine concentration (IC), water concentration (WC), CT value at 40 keV (CT40keV), the slope of energy spectral attenuation curve (λHU) and normalized iodine concentration (NIC) were measured and calculated by gemstone spectral imaging Viewer software. One-way ANOVA was used for the comparison of normal distribution DECT parameters between three levels for patients with NSCLC and patients with LAC. Non-normal distribution data were tested by non-parametric test. In addition, the receiver operating characteristic curve of statistically significant DECT parameters was drawn to distinguish the non-high-level and the high-level of Ki-67. Area under the curve (AUC), sensitivity, specificity was calculated to measure the diagnostic performance of parameter. Both in solid NSCLC and LAC, the IC, NIC, WC, λHU and CT40keV at VP in the high-level group were significantly lower than those in the middle- and low-level group respectively, and the WC at VP in the high-level group was significantly higher than that in the middle- and low-level group respectively (all P < .05). Receiver operating characteristic analysis showed that IC and λHU at VP performed better in distinguishing the high-level and the non-high-level of Ki-67 (NSCLC: AUC = 0.713 and 0.714 respectively; LAC: AUC = 0.705 and 0.706 respectively). Quantitative parameters of DECT provide a new non-invasive method for evaluating the proliferation of cancer cells in solid NSCLC and LAC.
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http://dx.doi.org/10.1097/MD.0000000000029444DOI Listing
August 2022

Effects of oceanographic environment on the distribution and migration of Pacific saury (Cololabis saira) during main fishing season.

Sci Rep 2022 Aug 9;12(1):13585. Epub 2022 Aug 9.

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.

The Pacific saury (Cololabis saira) is one of the most commercially important pelagic fishes in Asia-Pacific countries. The oceanographic environment, especially the Oyashio Current, significantly affects the distribution of Pacific saury, and may lead to variations in their migration route and the formation of fishing grounds in Japanese coastal region and the high seas. In this study, six oceanographic factors, sea surface temperature (SST), sea surface chlorophyll-a concentration (SSC), sea surface salinity (SSS), sea surface height (SSH), mixed layer depth (MLD), and eddy kinetic energy (EKE), were associated with the monthly catch per unit effort 1 (monthly CPUE, ton/vessel) and the monthly CPUE (ton/day) of Pacific saury from Chinese fishing vessels during the optimal fishing periods (September-November) in 2014-2017. The gradient forest analysis showed that the performance of monthly CPUE was higher than monthly CPUE and SST was the most important oceanographic factor influencing monthly CPUE, followed by EKE. The generalized additive model indicated that SST, SSH, and EKE negatively affected monthly CPUE, whereas SSC, SSS, and MLD induced dome-shaped increases in monthly CPUE. The distributions of fishing locations are likely to form along Offshore Oyashio current and meanders, especially in October and November. Synchronous trends in the relationship between the intrusion area of the Oyashio and relative abundance variation index suggest that an increase in the intrusion area of the Oyashio causes more Pacific saury to migrate to the Japanese coastal region, and vice versa. These findings extend our understanding of the effects of the oceanographic environment on Pacific saury.
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http://dx.doi.org/10.1038/s41598-022-17786-9DOI Listing
August 2022

Comparative Study of the Efficacy and Safety of Radical Surgery With or Without Hyperthermic Intraperitoneal Chemotherapy in Locally Advanced Gastric Cancer: A Propensity Score-Matching Analysis.

Ann Surg Oncol 2022 Aug 8. Epub 2022 Aug 8.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei Province, China.

Background: The effectiveness and safety of preventive hyperthermic intraperitoneal chemotherapy (HIPEC) for gastric cancer (GC) remain controversial. This study aimed to describe the safety and efficacy of radical surgery (RS) with or without HIPEC for patients with locally advanced GC (LAGC).

Methods: The study identified 394 patients with LAGC who underwent RS with or without HIPEC in China.

Results: Of the 394 patients, 146 received RS+HIPEC, and 248 received RS alone. The RS-HIPEC procedure improved the relapse-free survival (RFS) of the GC patients (2-year RFS, 62.9 % vs 37.8 %; χ = 4.468; P = 0.035) compared with those who received RS alone. The incidence of postoperative myelosuppression (Z = 4.077; P = 0.043) was higher in the RS+HIPEC group, whereas the incidence of wound complications was lower (Z = 4.077; P = 0.043). In the subgroup analysis, HIPEC improved the OS (2-year OS, 69.9 % vs 40.8 %; χ = 5.537; P =0.019) and RFS (2-year RFS, 65.6 % vs 33.3 %; χ = 7.380, P = 0.007) of the patients with nerve invasion and the RFS of the patients with vascular invasion (2-year RFS, 60.7 % vs 31.6 %; χ = 3.891; P = 0.049). In addition, the prognosis of the patients who underwent HIPEC was better when the tumor diameter was smaller than 5 cm (2-year RFS, 68.6 % vs 37.9 %; χ = 3.957; P = 0.047).

Conclusions: The RS + HIPEC procedure improved the RFS of the patients with LAGC compared with RS alone, especially the patients with nerve or vascular invasion and the patients with tumor smaller than 5 cm. Moreover, it reduced the incidence of wound complications and did not induce more perioperative complications in addition to myelosuppression.
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http://dx.doi.org/10.1245/s10434-022-12348-9DOI Listing
August 2022

Clinical Value of sTREM-1, PCT, and 1,3--D Glucan in Diagnosis of Immune-Associated Pulmonary Interstitial Disease with Fungal Infection.

Biomed Res Int 2022 27;2022:6095441. Epub 2022 Jul 27.

Department of Rheumatology and Immunology, Tianjin First Central Hospital, Tianjin 300192, China.

Background: Fungal infection in the lungs can cause fungal infectious diseases. This disease develops rapidly and involves a wide range. Pathogenic fungi are also more serious types of pathogenic bacteria. If it invades deep organs and tissues, it will endanger life, so it needs timely diagnosis.

Aim: To investigate the diagnostic value of serum soluble myeloid cell triggering receptor-1 (sTREM-1), procalcitonin (PCT), and 1,3--D glucan detection in immune related lung disease complicated with fungal infection.

Methods: In this study, a case-control study was conducted. 50 patients with immune-related pulmonary disease complicated with fungal infection (infection group) diagnosed by sputum culture in our hospital from January 2017 to December 2021 were selected as the control group, and 50 patients with immune-related pulmonary disease without fungal infection were selected as the control group. The levels of sTREM-1, PCT, and 1,3--D glucan were compared in the two groups. The receiver operating characteristic (ROC) was used to analyze the value of the three indicators in the diagnosis of immune-related pulmonary disease complicated with fungal infection, and the changes of the three indicators before and after treatment were compared.

Results: The levels of sTREM-1, PCT, and 1,3--D glucan in the infection group were higher than those in the control group ( < 0.05). The levels of sTREM-1, PCT, and 1,3--D glucan in the infection group after treatment were significantly lower than those before treatment ( < 0.05). The AUC value of sTREM-1 in the diagnosis of immune-related pulmonary diseases complicated with fungal infection was 0.980, the sensitivity was 97.11%, and the specificity was 83.06%. The AUC value of PCT in the diagnosis of immune-related pulmonary diseases complicated with fungal infection was 0.860, the sensitivity was 80.00%, and the specificity was 72.41%. The AUC value of 1,3--D glucan in the diagnosis of immune-related pulmonary diseases complicated with fungal infection was 0.993, the sensitivity was 98.74%, and the specificity was 99.16%. The levels of sTREM-1, PCT, and 1,3--D glucan in the infection group after treatment were considerably lower than those before treatment, and the difference was statistically significant ( < 0.05).

Conclusion: The detection of sTREM-1, PCT, and 1,3--D glucan levels has high clinical value for the diagnosis of immune-related pulmonary diseases complicated with fungal infection.
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http://dx.doi.org/10.1155/2022/6095441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348935PMC
August 2022

Association between diabetes complicated with comorbidities and frailty in older adults: A cross-sectional study.

J Clin Nurs 2022 Aug 7. Epub 2022 Aug 7.

West China School of Nursing, Sichuan University/Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, China.

Aims And Objectives: This study investigated the relationship between frailty and diabetes complicated with comorbidities.

Background: Frailty is a common geriatric syndrome, and older adults with diabetes are prone to frailty. Patients with diabetes and comorbidities might be at increased risk of developing frailty.

Design: A multicenter cross-sectional study.

Methods: A cross-sectional study was conducted to identify older patients with diabetes and comorbidities in the internal medicine departments of five tertiary general hospitals in Sichuan Province, China, from March 2020 to June 2021. We used the FRAIL scale to identify frailty, and multinomial logistic regression was used to compare sociodemographic characteristics and comorbidities of frail or pre-frail participants with robust participants. The STROBE checklist was used for this cross-sectional study.

Results: A total of 1652 patients (883 males, 53.5%) were included, and the prevalence of frailty was 26.5%. Multinomial logistic regression analysis revealed that compared with robust patients, diabetic patients with hypertension, coronary heart disease, chronic cardiac failure, COPD, cerebrovascular diseases, osteoarticular diseases, chronic renal diseases, chronic gastrointestinal diseases and cancer were more likely to be frail. In addition, patients who engaged in less exercise, presented more comorbidities, were older and had lower education levels, were more prone to frailty.

Conclusion: There was a clear correlation between diabetes complicated with comorbidities and the development of frailty. Appropriate personalised care levels for patients with diabetes and comorbidities, and early screening for frailty might reduce the prevalence of frailty in these patients.

Relevance To Clinical Practice: This study provided information for healthcare providers to identify circumstances that increase the risk of frailty and more effectively support patients with diabetes and comorbidities.
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http://dx.doi.org/10.1111/jocn.16442DOI Listing
August 2022

Retrospective Study of Effects of Preoperative BMI on Early Outcomes in Recipients After Kidney Transplant.

Transplant Proc 2022 Aug 4. Epub 2022 Aug 4.

Division of Life Sciences and Medicine, Department of Nursing, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China. Electronic address:

Background: The objective of this retrospective study was to establish the effect of the preoperative body mass index (BMI) on early outcomes of recipients after a kidney transplant, including liver and kidney function and fasting blood glucose recovery.

Methods: Our analytical cohort were patients who had undergone a kidney transplant at The First Affiliated Hospital of USTC [2016-2019]. The BMI classifications were underweight (<18.5 kg/m), normal weight (18.5-23.9 kg/m), and overweight or obese (≥24 kg/m). A χ test was conducted to compare differences between the patients in the different BMI groups.

Results: We enrolled 831 recipients in the study. The percentage of patients with normal serum creatinine and normal urea nitrogen in the BMI ≥24 group was lower at different periods after surgery (P<0.05). The percentage of patients with normal uric acid in the normal weight group was higher on day 1 and the first week after surgery (P < 0.001). In the first and second weeks postsurgery, the percentage of patients with aspartate transaminase/alanine aminotransaminase in the BMI ≥24 group was lower (P < .005). In the first week postsurgery, the percentage of patients with normal albumin/globulin in the normal weight group was higher (P < .05). No statistically significant difference among the 3 groups was found in the incidence of hyperglycemia (P > .05).

Conclusions: The patients who were overweight or obese preoperatively had poorer renal and liver functions postoperatively. Targeted interventions to control or mitigate rates of overweight or obesity preoperatively should be identified.
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http://dx.doi.org/10.1016/j.transproceed.2022.04.030DOI Listing
August 2022

Low-voltage ultrafast nonvolatile memory via direct charge injection through a threshold resistive-switching layer.

Nat Commun 2022 Aug 6;13(1):4591. Epub 2022 Aug 6.

Center for Nanochemistry, Beijing Science and Engineering Center for Nanocarbons, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

The explosion in demand for massive data processing and storage requires revolutionary memory technologies featuring ultrahigh speed, ultralong retention, ultrahigh capacity and ultralow energy consumption. Although a breakthrough in ultrafast floating-gate memory has been achieved very recently, it still suffers a high operation voltage (tens of volts) due to the Fowler-Nordheim tunnelling mechanism. It is still a great challenge to realize ultrafast nonvolatile storage with low operation voltage. Here we propose a floating-gate memory with a structure of MoS/hBN/MoS/graphdiyne oxide/WSe, in which a threshold switching layer, graphdiyne oxide, instead of a dielectric blocking layer in conventional floating-gate memories, is used to connect the floating gate and control gate. The volatile threshold switching characteristic of graphdiyne oxide allows the direct charge injection from control gate to floating gate by applying a nanosecond voltage pulse (20 ns) with low magnitude (2 V), and restricts the injected charges in floating gate for a long-term retention (10 years) after the pulse. The high operation speed and low voltage endow the device with an ultralow energy consumption of 10 fJ. These results demonstrate a new strategy to develop next-generation high-speed low-energy nonvolatile memory.
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http://dx.doi.org/10.1038/s41467-022-32380-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357017PMC
August 2022

Cloning and functional analysis of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) in Santalum album L.

Gene 2022 Aug 3:146762. Epub 2022 Aug 3.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China. Electronic address:

The commercial value of Santalum album L. lies in its aromatic heartwood and essential oil. Sesquiterpenes are the main components of sandal essential oil, and these are synthesized through the plant's mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. In this study, the first key rate-limiting enzyme, 1-deoxy-D-xylulose-5-phosphate synthase (SaDXS), was investigated to provide a theoretical molecular basis for the sandalwood MEP sesquiterpene biosynthetic pathway. The biofunctions of SaDXS were also analyzed. SaDXS promoters were successfully cloned from a seven-year-old S. album tree. SaDXS1A/1B promoter activity was verified by a β-glucuronidase (GUS) assay and by analyzing cis-acting elements of the promoters, which carried light- and methyl jasmonate (MeJA)-responsive signals. In an experiment involving yellow S. album seedlings, exposure to light upregulated SaDXS1A/1B expression and increased chlorophyll and carotenoid contents when overexpressed in Arabidopsis thaliana. Analysis of the expression of SaDXS1A/1B and SaSSy, key genes of santalol biosynthesis, revealed SaDXS1A expression in all tissues whereas SaDXS1B was expressed in tissues that contained photosynthetic pigments, such as stems, leaves and flowers. Sandal seedlings exogenously treated with two hormones, MeJA and ethylene, revealed similar expression patterns for SaDXS1A/1B and SaSSy. Sandal seedlings were treated with an inhibitor of DXS, clomazone, but showed no significant changes in the contents of α-santalene, β-santalene and α-santalol between treatment and control groups. These results suggest that SaDXS1A/1B play a role in the synthesis of sandalwood sesquiterpenes, providing carbon for downstream secondary metabolites. SaDXS1A/1B also play a role in the biosynthesis of chlorophyll, carotenoids, and primary metabolites.
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http://dx.doi.org/10.1016/j.gene.2022.146762DOI Listing
August 2022

The role of mitochondrial fission factor in podocyte injury in diabetic nephropathy.

Biochem Biophys Res Commun 2022 Jul 8;624:40-46. Epub 2022 Jul 8.

Department of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, 266000, Shandong, China. Electronic address:

Diabetic nephropathy (DN) is one of the most important complications of diabetes mellitus (DM) and has become the second cause of end-stage renal disease (ESRD). This study intends to investigate the molecular mechanism of increased mitochondrial fission in podocytes under the effect of high glucose (HG), and to preliminarily study the role of mitochondrial fission factor (MFF)-mediated mitochondrial fission in podocyte injury of DN. In vitro studies, we found that HG induced increased mitochondrial fission and podocyte damage. At the same time MFF mRNA and protein levels was increased, suggesting that MFF was transcriptional upregulated under HG conditions. Consistent with this, in vivo studies found that mitochondrial fission was also significantly increased in podocytes of diabetic nephropathy mice, and MFF expression was up-regulated. Therefore, our study proves that mitochondrial fission increases in podocytes under DM both in vitro and in vivo, and the up-regulation of MFF expression may be one of the reasons for the increase of mitochondrial fission. After inhibiting the expression of MFF, the survival rate of podocytes was significantly decreased under HG conditions, suggesting that MFF may play a protective role in podocyte injury in DN.
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http://dx.doi.org/10.1016/j.bbrc.2022.07.019DOI Listing
July 2022

The Effects of Serum Albumin Pre-Adsorption of Nanoparticles on Protein Corona and Membrane Interaction: A Molecular Simulation Study.

J Mol Biol 2022 Aug 2:167771. Epub 2022 Aug 2.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P. R. China. Electronic address:

As a platform to deliver imaging and therapeutic agents to targeted sites in vivo, nanoparticles (NPs) have widespread applications in diagnosis and treatment of cancer. However, the poor in vivo delivery efficiency of nanoparticles limits its potential for further application. Once enter the physiological environment, nanoparticles immediately interact with proteins and form protein corona, which changes the physicochemical properties of nanoparticle surface and further affects their transport. In this study, we performed molecular dynamics simulations to study the adsorption mechanism of nanoparticles with various surface modifications and different proteins (e.g., human serum albumin, complement protein C3b), and their interactions with cell membrane. The results show that protein human serum albumin prefers to interact with hydrophobic and positively charged nanoparticles, while the protein C3b prefers the hydrophobic and charged nanoparticles. The pre-adsorption of human serum albumin on the nanoparticle surface obviously decreases the interaction of nanoparticle with C3b. Furthermore, the high amount of protein pre-adsorption could decrease the probability of nanoparticle-membrane interaction. These results indicate that appropriate modification of nanoparticles with protein provides nanoparticles with better capability of targeting, which could be used to guide nanoparticle design and improve transport efficiency.
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http://dx.doi.org/10.1016/j.jmb.2022.167771DOI Listing
August 2022

N-mytistoyltransferase 1 and 2 are potential tumor suppressors and novel targets of miR-182 in human non-small cell lung carcinomas.

Lung Cancer 2022 Jul 29;171:70-81. Epub 2022 Jul 29.

Department of Clinical Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China. Electronic address:

Background & Aims: Non-small cell lung cancer (NSCLC) accounts for about 80% of lung cancer diagnoses across the world. Despite recent appreciable improvements in treatment plans for patients with NSCLC, the prognosis for those with the cancer still remains poor. Recently, a growing number of studies have shown that N-myristoyltransferases (NMTs) may be critical in carcinogenesis, however, the functional and clinical significance of this pathway in NSCLC remains unclear and requires further research.

Methods: Initially, we evaluated the expression levels of NMT1 or NMT2 in a clinical cohort comprising of 303 paired primary NSCLC tissues and matched normal mucosae by using ELISA. We subsequently performed a tissue microarray analysis (TMA) to confirm its expression pattern in an independent validation cohort (n = 78). Then, we used a publicly available KM plotter database (n = 1921) to evaluate the prognostic impact of NMT1 and NMT2 in NSCLC. Lastly, a series of in-vitro molecular/cellular and animal experiments were performed for mechanistic understanding of the role of N-myristoyltransferases in NSCLC.

Results: Our ELISA data revealed that the expression level of NMT1 and NMT2 was down-regulated in tumor tissues (n = 303, P < 0.0001), which was confirmed in an independent validation cohort by TMA (n = 78, P = 0.014 for NMT1 and P < 0.0001 for NMT2). On the other hand, patients with low expression of NMT1 or NMT2 had shorter overall survival (P = 0.013, HR = 0.85 for NMT1; P = 0.00059, HR = 0.8, for NMT2). Mechanistically, we revealed that the interaction and co-localization of NMT1 and NMT2 in NSCLC, and N-terminus of NMT1 and NMT2 was observed to be crucial for their interaction as well as for their catalytic activity. Moreover, we found that NMT1 can significantly promote the expression of NMT2 by enhancing its stability. We corroborated these findings by performing functional assays in which the knockout of NMT1 and NMT2 resulted in enhanced cell proliferation, migration and invasion as well as increased tumorxenograftgrowth. In addition, we identified miR-182 as a novel regulator of both NMT1 and NMT2. More specifically, the overexpression or inhibition of miR-182 modulated globe N-myristoylation level, contributed to phenotypic alterations in NSCLS cells.

Conclusions: NMT1 and NMT2 can act as potential tumor suppressors in NSCLC, and the inhibition of miR-182 expression or therapeutic NMTs replenishment may be a promising treatment option for patients with NSCLC.
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http://dx.doi.org/10.1016/j.lungcan.2022.07.021DOI Listing
July 2022

Editorial: Food Protein-Based Colloids: Structure, Digestion, and Nutrients Delivery.

Front Nutr 2022 18;9:982366. Epub 2022 Jul 18.

College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China.

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http://dx.doi.org/10.3389/fnut.2022.982366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344554PMC
July 2022

Computer-aided diagnostic models to classify lymph node metastasis and lymphoma involvement in enlarged cervical lymph nodes using PET/CT.

Med Phys 2022 Aug 4. Epub 2022 Aug 4.

Department of Biotherapy and Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Guoxue Road 37, Chengdu, 610041, China.

Background: It is a clinical problem to identify histological component in enlarged cervical lymph nodes, particularly in differentiation between lymph node metastasis and lymphoma involvement.

Purpose: To construct two kinds of deep learning (DL) based computer-aided diagnosis (CAD) systems including DL-convolutional neural networks (DL-CNN) and DL-machine learning for pathological diagnosis of cervical lymph nodes by PET/CT images.

Methods: We collected CT, PET, and PET/CT images series from 165 patients with enlarged cervical lymph nodes receiving examinations from January 2014 to June 2018. Six CNNs pretrained on ImageNet as DL architectures were used for two kinds of DL-based CAD models, including DL-CNN and DL-machine learning models. The DL-CNN models were constructed via transfer learning for classification of lymphomatous and metastatic lymph nodes. The DL-machine learning models were developed by DL-based features extractors and SVM classifier. As for DL-SVM models, we also evaluate the effect of handcrafted radiomics features in combination of DL-based features.

Results: The DL-CNN model with ResNet50 architecture on PET/CT images had the best diagnostic performance among all six algorithms with an area under the receiver operating characteristic curve (AUC) of 0.845 and accuracy of 78.13% in the testing cohort. The DL-SVM model on ResNet50 extractor showed great performance for the testing cohort with an AUC of 0.901, accuracy of 86.96%, sensitivity of 76.09%, and specificity of 94.20%. The combination of DL-based and handcrafted features yielded the improvement of diagnostic performance.

Conclusions: Our DL-based CAD systems on PET/CT images were developed for classifying metastatic and lymphomatous involvement with favorable diagnostic performance in enlarged cervical lymph nodes. The further clinical practice of our systems may improve quality of the following therapeutic interventions and optimize patients' outcomes. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mp.15901DOI Listing
August 2022

Carbon Nanotubes/Polydopamine/ZSM-5 Composite Soil Conditioner with Good Controlled Release and Adsorption Properties.

Langmuir 2022 Aug 4. Epub 2022 Aug 4.

Jiangsu LuHong Landscaping Engineering Company Limited, Jiangsu 226100, P. R. China.

Currently, the excessive application of fertilizers and the random discharge of waste water, waste gas, and residues have led to more and more serious soil pollution problems. Zeolite is the most promising material for preparing a green and environmentally friendly soil conditioner. Herein, the carbon nanotubes/polydopamine/ZSM-5 composite soil conditioner was prepared by a facile two-step method, and it was used to release fulvic acid and adsorb methylene blue to improve the environment. The cumulative release rate of the composite soil conditioner was 52% within 430 h for fulvic acid, which had a good sustained release effect and could be sustained-released in different acid-based surroundings. In addition, it showed a good adsorption capacity of methylene blue, and it is about 80.02 mg/g which was about six times higher than that of ZSM-5. It was beneficial for the adsorption of methylene blue in a neutral environment. Finally, it could promote the growth of brassica chinensis and maize, and the promotion effect was 60 and 35%, respectively. Therefore, the carbon nanotubes/polydopamine/ZSM-5 composite soil conditioner is a green and efficient material, which provides a new strategy to solve the problem of soil pollution.
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http://dx.doi.org/10.1021/acs.langmuir.2c01175DOI Listing
August 2022

Beneficial effect on the soil microenvironment of Trichoderma applied after fumigation for cucumber production.

PLoS One 2022 2;17(8):e0266347. Epub 2022 Aug 2.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Biocontrol agents applied after fumigation play an important role to the soil microenvironment. We studied the effect of Trichoderma applied after dimethyl disulfide (DMDS) plus chloropicrin (PIC) fumigation on the cucumber growth, soil physicochemical properties, enzyme activity, taxonomic diversity, and yield through laboratory and field experiments. The results confirmed that Trichoderma applied after fumigation significantly improved soil physicochemical properties, cucumber growth, soil-borne pathogens, and soil enzyme activity. Genetic analysis indicated that Trichoderma applied after fumigation significantly increased the relative abundance of Pseudomonas, Humicola and Chaetomium, and significantly decreased the relative abundance of the pathogens Fusarium spp. and Gibberella spp., which may help to control pathogens and enhanced the ecological functions of the soil. Moreover, Trichoderma applied after fumigation obviously improved cucumber yield (up to 35.6%), and increased relative efficacy of soil-borne pathogens (up to 99%) and root-knot nematodes (up to 96%). Especially, we found that Trichoderma applied after fumigation increased the relative abundance of some beneficial microorganisms (such as Sodiomyces and Rhizophlyctis) that can optimize soil microbiome. It is worth noting that with the decline in the impact of the fumigant, these beneficial microorganisms still maintain a higher abundance when the cucumber plants were uprooted. Importantly, we found one tested biocontrol agent Trichoderma 267 identified and stored in our laboratory not only improved cucumber growth, reduced soil-borne diseases in late cucumber growth stages but also optimized micro-ecological environment which may have good application prospect and help to keep environmental healthy and sustainable development.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0266347PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345367PMC
August 2022

Sous-vide cooking endows a better microstructure for hairtail (Trichiurus lepturus) than traditional cooking: Mechanisms of moisture migration.

J Food Sci 2022 Aug 1. Epub 2022 Aug 1.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Sous-vide cooking is a highly praised method used to cook muscle foods because of its desired effect of providing better sensory properties by maintaining texture. In this study, we further explored the effect of water on texture by revealing the mechanisms of moisture migration. Low field nuclear magnetic resonance (LF-NMR) showed that the nonflowing water in sous-vide cooking hairtail was 2.36 ± 0.33% higher than that in traditional cooking. Magnetic resonance imaging (MRI) was used to clarify the law of moisture migration induced by temperature, and the moisture migration of the sous-vide cooking hairtail was slower during the holding heating stage. The microstructure explained the change rules of the texture. The degree of change was consistent with the moisture migration level. Digitalizing analysis quantitatively verified the effect of sous-vide cooking on the hairtail microstructure. The low moisture migration rate of sous-vide cooking resulted in a less damaged microstructure of the hairtail, manifesting as a desirable texture. PRACTICAL APPLICATION: LF-NMR and MRI showed that sous-vide hairtails exhibited a lower moisture migration rate. The holding heating stage only slightly changed the microstructure of the hairtail. The digitalizing analysis confirmed the moisture migration mechanisms. Heat-induced protein denaturation was closely related to the water state.
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http://dx.doi.org/10.1111/1750-3841.16260DOI Listing
August 2022

Multi-View Feature Enhancement Based on Self-Attention Mechanism Graph Convolutional Network for Autism Spectrum Disorder Diagnosis.

Front Hum Neurosci 2022 15;16:918969. Epub 2022 Jul 15.

School of Computer Science and Technology, Shandong Technology and Business University, Yantai, China.

Functional connectivity (FC) network based on resting-state functional magnetic resonance imaging (rs-fMRI) has become an important tool to explore and understand the brain, which can provide objective basis for the diagnosis of neurodegenerative diseases, such as autism spectrum disorder (ASD). However, most functional connectivity (FC) networks only consider the unilateral features of nodes or edges, and the interaction between them is ignored. In fact, their integration can provide more comprehensive and crucial information in the diagnosis. To address this issue, a new multi-view brain network feature enhancement method based on self-attention mechanism graph convolutional network (SA-GCN) is proposed in this article, which can enhance node features through the connection relationship among different nodes, and then extract deep-seated and more discriminative features. Specifically, we first plug the pooling operation of self-attention mechanism into graph convolutional network (GCN), which can consider the node features and topology of graph network at the same time and then capture more discriminative features. In addition, the sample size is augmented by a "sliding window" strategy, which is beneficial to avoid overfitting and enhance the generalization ability. Furthermore, to fully explore the complex connection relationship among brain regions, we constructed the low-order functional graph network (Lo-FGN) and the high-order functional graph network (Ho-FGN) and enhance the features of the two functional graph networks (FGNs) based on SA-GCN. The experimental results on benchmark datasets show that: (1) SA-GCN can play a role in feature enhancement and can effectively extract more discriminative features, and (2) the integration of Lo-FGN and Ho-FGN can achieve the best ASD classification accuracy (79.9%), which reveals the information complementarity between them.
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http://dx.doi.org/10.3389/fnhum.2022.918969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334869PMC
July 2022

High CHAF1A Expression Levels Are Positively-Correlated with PD-L1 Expression and Indicate Poor Prognosis in Gastric Cancer.

Evid Based Complement Alternat Med 2022 19;2022:1323321. Epub 2022 Jul 19.

The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institutes of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou 310022, China.

Objective: The aim of this study was to analyze the association between the expression of chromatin assembly factor 1 subunit A (CHAF1A) in gastric cancer (GC) and clinicopathological features, disease prognosis, and expression of programmed cell death-ligand 1 (PD-L1). . A total of 140 GC tissue specimens were collected between January 2013 and December 2017. CHAF1A expression in GC and paracancerous tissues was determined. Then, the associations between CHAF1A expression level in the collected tissues and clinicopathological features as well as PD-L1 expression level were investigated. Cox regression analyses were carried out to determine whether CHAF1A is an independent prognostic factor for GC. Finally, the association between CHAF1A expression levels and survival of the GC patients was investigated.

Results: A significantly higher level of CHAF1A expression in GC tissues was found compared to that in paracancerous tissues (=0.042). CHAF1A expression level in GC tissues was found to be strongly associated with family history (=0.005), smoking history (=0.016), T stage (=0.001), tumor marker AFP (=0.017), tumor marker CEA (=0.027), and PD-L1 expression (=0.029). CHAF1A expression was also found to be positively correlated to PD-L1 expression (=0.012). Moreover, high CHAF1A expression levels were found to lead to poor prognosis (=0.019). Univariate and multivariate analyses all showed that CHAF1A was an independent poorer prognostic factor for gastric cancer (=0.021, HR = 1.175, 95% CI: 1.090-2.890 for univariate analyses; =0.014, HR = 2.191, 95% CI:1.170-4.105 for multivariate analyses). A high level of CHAF1A expression was thus found to be an independent risk factor for GC prognosis.

Conclusion: High CHAF1A expression is associated with poor GC prognosis and positively correlated to PD-L1 expression. Thus, CHAF1A expression level may be used as a novel biomarker for GC diagnosis.
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http://dx.doi.org/10.1155/2022/1323321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325625PMC
July 2022

Optimization of Fracturing Fluid and Retarded Acid for Stimulating Tight Naturally Fractured Bedrock Reservoirs.

ACS Omega 2022 Jul 14;7(29):25122-25131. Epub 2022 Jul 14.

State Key Laboratory of Oil and Gas Resources and Prospecting, China University of Petroleum at Beijing, Beijing 102249, China.

In tight naturally fractured bedrock reservoirs, hydrocarbons are typically stored in fractures, where hydraulic fracturing is needed to connect these fractures to the wellbore. The cross-linked gel is used as the fracturing fluid to reduce the fluid leak-off through natural fractures; however, it can cause formation damage due to its high content of residues after breaking. A synthetic polymer is introduced and evaluated that can maintain a high viscosity to minimize the leak-off , while having a low residue content after breaking. To further enhance the conductivity of the created fracture network, acid is applied to etch and roughen the created fracture faces. Because the target reservoir has a complex mineral composition, a three-step coreflood sequence using reservoir rock samples with controlled fracture widths is established to quantify the enhancement of different retarded acids and to reveal the mechanism behind it. The results indicate the synergy effect of reducing the acid concentration and surfactant adsorption on rock surfaces can lead to an obvious enhancement of the fracture permeability after acidizing, while the mud acid or hydrofluoric acid is not suitable for the target reservoir where concentrations of silicates and clays are relatively high.
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http://dx.doi.org/10.1021/acsomega.2c01612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330227PMC
July 2022

Disruption of REC8 in Meiosis I led to watermelon seedless.

Plant Sci 2022 Jul 26:111394. Epub 2022 Jul 26.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

In triploid watermelon (Citrullus lanatus), the homologous chromosomes of germ cells are disorder during meiosis, resulting in the failure of seeds formation and producing seedless fruit. Therefore, mutating the genes specifically functioning in meiosis may be an alternative way to achieve seedless watermelon. REC8, as a key component of the cohesin complex in meiosis, is dramatically essential for sister chromatid cohesion and chromosome segregation. However, the role of REC8 in meiosis has not yet been characterized in watermelon. Here, we identified ClREC8 as a member of RAD21/REC8 family with a high expression in male and female flowers of watermelon. In situ hybridization analysis showed that ClREC8 was highly expressed at the early stage of meiosis during pollen formation. Knocking out ClREC8 in watermelon led to decline of pollen vitality. After pollinating with foreign normal pollen, the ovaries of ClREC8 knockout lines could inflate normally but failed to form seeds. We further compared the meiosis chromosomes of pollen mother cells in different stages between the knockout lines and the corresponding wild type. The results indicated that ClREC8 was required for the monopolar orientation of the sister kinetochores in Meiosis I. Additionally, transcriptome sequencing (RNA-seq) analysis between WT and the knockout lines revealed that the disruption of ClREC8 caused the expression levels of mitosis-related genes and meiosis-related genes to decrease. Our results demonstrated ClREC8 has a specific role in Meiosis I of watermelon germ cells, and loss-of-function of the ClREC8 led to seedless fruit, which may provide an alternative strategy to breed cultivars with seedless watermelon.
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http://dx.doi.org/10.1016/j.plantsci.2022.111394DOI Listing
July 2022

Uncovering the role of impurity sugars on the crystallization of d-tagatose crystal: Experiments and molecular dynamics simulations.

Food Chem 2022 Jul 22;397:133762. Epub 2022 Jul 22.

School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, 300072 Tianjin, PR China; The Co-Innovation Center of Chemistry and Chemical Engineering of Tianjin, 300072 Tianjin, PR China.

Impurity sugars produced in upstream process of functional sugars are significantly impacting the product quality. In this work, the effect of congeners (d-maltose (d-MAL), d-fructose (d-FRU), d-glucose (d-GLU)) on primary and secondary nucleation of d-tagatose (d-TAG) crystals was investigated. The impurity sugars showed an inhibition on primary nucleation of d-TAG crystals, while a promotion on the secondary nucleation of d-TAG. Interestingly, the impact of impurity sugars on d-TAG crystal growth was similar to that on primary nucleation. The diffusion ability, hydrogen bonding forming ability, interaction energy of d-TAG crystal surfaces and impurity sugars were evaluated by molecular dynamics (MD) simulations to reveal the nucleation and growth behavior. Based on the above findings, we designed the d-TAG crystallization experiments, and obtained d-TAG crystals with uniform particle size distribution and regular morphology. This study helps to understand the influence of impurity sugars on crystallization, guiding the industrial manufacturing of functional sugars.
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http://dx.doi.org/10.1016/j.foodchem.2022.133762DOI Listing
July 2022

Realization of an Error-Correcting Surface Code with Superconducting Qubits.

Phys Rev Lett 2022 Jul;129(3):030501

Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.

Quantum error correction is a critical technique for transitioning from noisy intermediate-scale quantum devices to fully fledged quantum computers. The surface code, which has a high threshold error rate, is the leading quantum error correction code for two-dimensional grid architecture. So far, the repeated error correction capability of the surface code has not been realized experimentally. Here, we experimentally implement an error-correcting surface code, the distance-three surface code which consists of 17 qubits, on the Zuchongzhi 2.1 superconducting quantum processor. By executing several consecutive error correction cycles, the logical error can be significantly reduced after applying corrections, achieving the repeated error correction of surface code for the first time. This experiment represents a fully functional instance of an error-correcting surface code, providing a key step on the path towards scalable fault-tolerant quantum computing.
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http://dx.doi.org/10.1103/PhysRevLett.129.030501DOI Listing
July 2022

Assessment of mitochondrial dysfunction and implications in cardiovascular disorders.

Life Sci 2022 Jul 25:120834. Epub 2022 Jul 25.

Key Laboratory of Medical Electrophysiology, Ministry of Education, Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China. Electronic address:

Mitochondria play a pivotal role in cellular function, not only acting as the powerhouse of the cell, but also regulating ATP synthesis, reactive oxygen species (ROS) production, intracellular Ca cycling, and apoptosis. During the past decade, extensive progress has been made in the technology to assess mitochondrial functions and accumulating evidences have shown that mitochondrial dysfunction is a key pathophysiological mechanism for many diseases including cardiovascular disorders, such as ischemic heart disease, cardiomyopathy, hypertension, atherosclerosis, and hemorrhagic shock. The advances in methodology have been accelerating our understanding of mitochondrial molecular structure and function, biogenesis and ROS and energy production, which facilitates new drug target identification and therapeutic strategy development for mitochondrial dysfunction-related disorders. This review will focus on the assessment of methodologies currently used for mitochondrial research and discuss their advantages, limitations and the implications of mitochondrial dysfunction in cardiovascular disorders.
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http://dx.doi.org/10.1016/j.lfs.2022.120834DOI Listing
July 2022

The Exosome Journey: From Biogenesis to Regulation and Function in Cancers.

J Oncol 2022 18;2022:9356807. Epub 2022 Jul 18.

Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China.

Exosomes are a type of small endosomal-derived vesicles ranging from 30 to 150 nm, which can serve as functional mediators in cell-to-cell communication and various physiological and pathological processes. In recent years, exosomes have emerged as crucial mediators of intracellular communication among tumor cells, immune cells, and stromal cells, which can shuttle bioactive molecules, such as proteins, lipids, RNA, and DNA. Exosomes exhibit the high bioavailability, biological stability, targeting specificity, low toxicity, and immune characteristics, suggesting their potentials in the diagnosis and treatment of cancers. They can be applied as an effective tool in the diagnostics, therapeutics, and drug delivery in cancers. This review summarizes the regulation and functions of exosomes in various cancers to augment our understanding of exosomes, which paves the way for parallel advancements in the therapeutic approach of cancers. In this review, we also discuss the challenges and prospects for clinical application of exosome-based diagnostics and therapeutics for cancers.
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http://dx.doi.org/10.1155/2022/9356807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313905PMC
July 2022

Improvement of Storage Stability of Zein-Based Pickering Emulsions by the Combination of Konjac Glucomannan and L-Lysine.

Front Nutr 2022 11;9:955272. Epub 2022 Jul 11.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.

In this work, L-lysine (Lys) was employed together with konjac glucomannan (KGM) to fabricate zein colloidal particles (ZCPs) aimed at enhancing the storage stability of Pickering emulsions. With the addition of Lys, zein-Lys colloidal particles (ZLCPs) and zein-Lys-KGM (ZLKCPs) exhibited smaller particle size (133.64 ± 1.43, 162.54 ± 3.51 nm), polydispersity index (PDI) (0.10 ± 0.029, 0.13 ± 0.022), π value, and more adsorbed protein. Meanwhile, KGM underwent deamidation in an alkaline solution, so the emulsions stabilized by ZLKCPs exhibited a solid gel-like structure with higher storage modulus (G') and loss modulus (G''), leading to lower fluidity and better stability. The synergistic effects of Lys and KGM improved the stability of the emulsion. Hydrophobic interactions and hydrogen bonds were the main driving forces forming colloidal particles, which were determined by driving force analysis.
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http://dx.doi.org/10.3389/fnut.2022.955272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309815PMC
July 2022

The Association Between Circulating Trans Fatty Acids and Thyroid Function Measures in U.S. Adults.

Front Endocrinol (Lausanne) 2022 8;13:928730. Epub 2022 Jul 8.

Department of Clinical Nutrition, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: There has been controversial evidence regarding the effect of trans fatty acids (TFAs) on thyroid function in animal studies, and the epidemiological studies are lacking. We aimed to investigate the potential associations between circulating TFAs and thyroid function biomarkers in a U.S. adult population sample.

Methods: We performed a cross-sectional survey with 626 adults aged ≥20 years who participated in the National Health and Nutrition Examination Survey (NHANES) 2009-2010. Multivariable linear regression models were constructed to elucidate the relationships between circulating concentrations of TFAs (palmitelaidic acid, vaccenic acid, elaidic acid, linoelaidic acid and the sum of the four TFAs) and a panel of thyroid function measures.

Results: For 626 adults, positive associations were found between palmitelaidic acid, elaidic acid and total thyroxine (TT4), between palmitelaidic acid and total triiodothyronine (TT3), and between linolelaidic acid and thyroid stimulating hormone (TSH), while linolelaidic acid was negatively associated with free thyroxine (FT4) (all P<0.05). Besides, the four TFAs and the sum TFAs were positively associated with free triiodothyronine (FT3). Vaccenic acid, elaidic acid, linoelaidic acid and the sum TFAs were positively associated with FT3/FT4, while the four TFAs and the sum TFAs were negatively associated with FT4/TT4 (all P<0.05). In stratified analysis, the associations between thyroid function measures and the ratios remained significant in female. For men, linolelaidic acid was negatively associated with FT4 and elaidic acid and the sum TFAs were positively associated with FT3. Furthermore, the associations between TFAs and FT3/FT4 remained significant.

Conclusion: Our findings revealed that TFAs exposure was associated with serum biomarkers of thyroid function. More researches are needed to evaluate the long-term health outcomes of these findings.
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http://dx.doi.org/10.3389/fendo.2022.928730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309269PMC
July 2022

A WRKY Protein, MfWRKY40, of Resurrection Plant Plays a Positive Role in Regulating Tolerance to Drought and Salinity Stresses of .

Int J Mol Sci 2022 Jul 24;23(15). Epub 2022 Jul 24.

Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.

WRKY transcription factors (TFs), one of the largest transcription factor families in plants, play an important role in abiotic stress responses. The resurrection plant, , has a strong tolerance to dehydration, but only a few WRKY proteins related to abiotic stress response have been identified and functionally characterized in . In this study, we identified an early dehydration-induced gene, , of . The deduced MfWRKY40 protein has a conserved WRKY motif but lacks a typical zinc finger motif in the WRKY domain and is localized in the nucleus. To investigate its potential roles in abiotic stresses, we overexpressed in and found that transgenic lines exhibited better tolerance to both drought and salt stresses. Further detailed analysis indicated that MfWRKY40 promoted primary root length elongation and reduced water loss rate and stomata aperture (width/length) under stress, which may provide the better water uptake and retention abilities. MfWRKY40 also facilitated osmotic adjustment under drought and salt stresses by accumulating more osmolytes, such as proline, soluble sugar, and soluble protein. Additionally, the antioxidation ability of transgenic lines was also significantly enhanced, represented by higher chlorophyll content, less malondialdehyde and reactive oxygen species accumulations, as well as higher antioxidation enzyme activities. All these results indicated that MfWRKY40 might positively regulate tolerance to drought and salinity stresses. Further investigation on the relationship of the missing zinc finger motif of MfWRKY40 and its regulatory role is necessary to obtain a better understanding of the mechanism underlying the excellent drought tolerance of .
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http://dx.doi.org/10.3390/ijms23158145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330732PMC
July 2022

Abaloparatide excels teriparatide in protecting against alveolar bone loss in experimental periodontitis.

J Periodontol 2022 Jul 26. Epub 2022 Jul 26.

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background: It was aimed to compare the effects of two osteoanabolic drugs, abaloparatide (ABL) and teriparatide (TPTD), on protecting alveolar bone in experimental periodontitis.

Methods: Twenty-four 9-week-old male Sprague-Dawley rats were placed with silk ligature around the right maxillary second molar, and then were randomly divided into 3 groups, i.e., the ABL, TPTD and saline group, receiving intermittent subcutaneous injections of ABL (80 ug/kg), TPTD (80 ug/kg) or saline respectively every other day for 4 weeks. Samples on both sides were assessed through micro-computerized tomography, histological and immunohistochemical analysis. Mouse pre-osteoblast MC3T3 cell was cultured with lipopolysaccharide (LPS) and treated with ABL or TPTD, before assays of cell proliferation, alkaline phosphatase (ALP) activity and real-time polymerase chain reaction.

Results: On the ligature side, both ABL and TPTD significantly reduced alveolar bone loss, and ABL had significantly better effects with higher expression of runt-related transcription factor 2 (RUNX2) and Bglap (formerly called osteocalcin); meanwhile, the ligature induced osteoclastogenesis and down-regulation of osteoprotegerin (OPG) was affected by neither drug. On the non-ligature side, ABL also showed better osteoanabolic effects. In vitro studies revealed that in presence of LPS, ABL and TPTD similarly promoted MC3T3 proliferation, whilst ABL induced higher ALP activity and osteoblastic gene expression compared to TPTD.

Conclusion: Both ABL and TPTD protect and regenerate alveolar bone in experimental periodontitis, and ABL behaves even better than TPTD at the same dose, attributed to its stronger osteoanabolic effects in this context. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/JPER.22-0211DOI Listing
July 2022
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