Publications by authors named "Yuan Gao"

2,096 Publications

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Bimetallic MOFs-derived coral-like Ag-MoC/C interwoven nanorods for amperometric detection of hydrogen peroxide.

Mikrochim Acta 2021 Jun 23;188(7):234. Epub 2021 Jun 23.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin, 150080, People's Republic of China.

Coral-like Ag-MoC/C-I and blocky Ag-MoC/C-II composites were obtained from one-step in situ calcination of [Ag(HL)(MoO)]·nHO [L: N-(pyridin-3-ylmethyl) pyridine-2-amine] under N/H and N atmospheres, respectively. The coral-like morphology of Ag-MoC/C-I is composed of interwoven nanorods embedded with small particles, and the nano-aggregate of Ag-MoC/C-II is formed by cross-linkage of irregular nanoparticles. The above composites are decorated on glassy carbon electrode (GCE) drop by drop to generate two enzyme-free electrochemical sensors (Ag-MoC/C/GCE) for amperometric detection of HO. In particular, the coral-like Ag-MoC/C-I/GCE sensor possesses rapid response (1.2 s), high sensitivity (466.2 μA·mM·cm), and low detection limit (25 nM) towards trace HO and has wide linear range (0.08 μM~4.67 mM) and good stability. All these sensing performances are superior to Ag-MoC/C-II/GCE, indicating that the calcining atmosphere has an important influence on microstructure and electrochemical properties. The excellent electrochemical HO sensing performance of Ag-MoC/C-I/GCE sensor is mainly attributed to the synergism of unique microstructure, platinum-like electron structure of MoC, strong interaction between Mo and Ag, as well as the increased active sites and conductivity caused by co-doped Ag and carbon. Furthermore, this sensor has been successfully applied to the detection of HO in human serum sample, contact lens solution, and commercial disinfector, demonstrating the potential in related fields of environment and biology. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-021-04888-wDOI Listing
June 2021

Cys183 and Cys258 in Cry49Aa toxin from Lysinibacillus sphaericus are essential for toxicity to Culex quinquefasciatus larvae.

Arch Microbiol 2021 Jun 23. Epub 2021 Jun 23.

College of Life Sciences, Gannan Normal University, Shida South Road 1, Ganzhou City, Jiangxi Province, 341000, People's Republic of China.

The two-component Cry48Aa/Cry49Aa toxin produced by Lysinibacillus sphaericus shows specifically toxic to Culex quinquefasciatus mosquito larvae. Cry49Aa C-terminal domain is responsible for specific binding to the larval gut cell membrane, while its N-terminal domain is required for interaction with Cry48Aa. To investigate functional role of cysteine in Cry49Aa, four cysteine residues at positions 70, 91, 183, and 258 were substituted by alanine. All mutants showed similar crystalline morphology and comparable yield to that of the wild type except that the yield of the C91A mutant was low. Four cysteine residues did not involve in disulfide bond formation within or between Cry49Aa molecules. Cys91, Cys183, and Cys258 are essential for larvicidal activity against C. quinquefasciatus larvae, while Cys70 is not. Substitution at C91, C183, and C258 caused weaker Cry48Aa- Cry49Aa interaction, while mutations at C183 and C258 reduced the binding capacities to the larval gut cell membrane. Thus, Cysteine residues at position 91, 183, and 258 in Cry49Aa are required for full toxicity of Cry48Aa/Cry49Aa toxin.
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http://dx.doi.org/10.1007/s00203-021-02436-xDOI Listing
June 2021

Novel Mathematical Model for Transient Pressure Analysis of Multifractured Horizontal Wells in Naturally Fractured Oil Reservoirs.

ACS Omega 2021 Jun 31;6(23):15205-15221. Epub 2021 May 31.

Petroleum Engineering Department, Khalifa University of Science and Technology, P.O. Box 2533, Abu Dhabi, United Arab Emirates.

Multifractured horizontal wells have gained significant attention within the petroleum industry for commercial development. Despite considerable developments of transient pressure analysis or flow rate behaviors for horizontal wells in naturally fractured reservoirs, some significant problems are yet to be resolved, including high heterogeneity of reservoirs, pressure sensitivity of hydraulic fractures, and non-Darcy flow effect, which may occur during the production life. This paper presents a more pragmatic mathematical model for multifractured horizontal wells in naturally fractured reservoirs based on the fractal system, the theory of permeability modulus, and the time-fractional calculus correspondingly as an extension of the classic trilinear flow model. This new model comprises three modules: high heterogeneity of the reservoir based on the fractal system, the permeability modulus typically showing the pressure sensitivity of hydraulic fractures, and the anomalous diffusion describing non-Darcy flow turbulence. This investigation evaluates a trilinear dual-permeability dual-porosity flow model, with the dual-porosity model for the unstimulated outer reservoir flow region, the dual-permeability model for the stimulated inner reservoir flow region, and the permeability modulus for the flow region of hydraulic fractures. The comprehensive sensitivity analysis conducted indicates how the key parameters, such as fractal dimension, hydraulic fracture permeability modulus and conductivity, interporosity flow coefficient, storativity ratio, etc., affect the transient pressure behaviors, along with their reasons for the change in behavior. Application to a field case study further demonstrates the validity of the mathematical model, and the results presented may play a guiding role in well test interpretation.
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http://dx.doi.org/10.1021/acsomega.1c01464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210455PMC
June 2021

Puerarin-Na Chelate Hydrate Simultaneously Improves Dissolution and Mechanical Behavior.

Mol Pharm 2021 Jun 18. Epub 2021 Jun 18.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, PR China.

Puerarin monohydrate (PUEM), as the commercial solid form of the natural anti-hypertension drug puerarin (PUE), has low solubility, poor flowability, and mechanical properties. In this study, a novel solid form as PUE-Na chelate hydrate was prepared by a reactive crystallization method. Crystal structure analysis demonstrated that PUE-Na contains PUE, Na, and water in a molar ratio of 1:1:7. It crystallizes in the monoclinic space group 2, and Na is linked with PUE and four water molecules through Na ← O coordination bonds. Another three crystal water molecules occupy channels along the crystallographic -axis. Observing along the -axis, the crystal structure features a distinct tubular helix and a DNA-like twisted helix. The complexation between Na and PUE in aqueous solution was confirmed by the Na selective electrode, indicating that PUE-Na chelate hydrate belongs to a type of chelate rather than organic metal salt. Compared with PUEM, PUE-Na exhibited a superior dissolution rate (i.e., ∼38-fold increase in water) owing to its lower solvation free energy and clear-enriched exposed polar groups. Moreover, PUE-Na enhanced the tabletability and flowability of PUEM, attributing to its better elastoplastic deformation and lower-friction crystal habit. The unique PUE-Na chelate hydrate with significantly enhanced pharmaceutical properties is a very promising candidate for future product development of PUE.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00005DOI Listing
June 2021

DNA methylome reveals cellular origin of cell-free DNA in spent medium of human preimplantation embryos.

J Clin Invest 2021 Jun;131(12)

Beijing Advanced Innovation Center for Genomics, Department of Obstetrics and Gynecology, and.

The discovery of embryonic cell-free DNA (cfDNA) in spent embryo culture media (SECM) has brought hope for noninvasive preimplantation genetic testing. However, the cellular origins of SECM cfDNA are not sufficiently understood, and methods for determining maternal DNA contamination are limited. Here, we performed whole-genome DNA methylation sequencing for SECM cfDNA. Our results demonstrated that SECM cfDNA was derived from blastocysts, cumulus cells, and polar bodies. We identified the cumulus-specific differentially methylated regions (DMRs) and oocyte/polar body-specific DMRs, and established an algorithm for deducing the cumulus, polar body, and net maternal DNA contamination ratios in SECM. We showed that DNA methylation sequencing accurately detected chromosome aneuploidy in SECM and distinguished SECM samples with low and high false negative rates and gender discordance rates, after integrating the origin analysis. Our work provides insights into the characterization of embryonic DNA in SECM and provides a perspective for noninvasive preimplantation genetic testing in reproductive medicine.
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http://dx.doi.org/10.1172/JCI146051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203451PMC
June 2021

Effect of Ru Doping on the Properties of LiFePO/C Cathode Materials for Lithium-Ion Batteries.

ACS Omega 2021 Jun 25;6(22):14122-14129. Epub 2021 May 25.

The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing 400040, People's Republic of China.

Doping of metals is highly effective in improving electrochemical performance of lithium iron phosphate. Here, based on a first-principles calculation result that Ru doping at the Fe sites has positive effects on promoting the ability of electron and Li transmission by reducing the lattice parameter and band gap, as well as the increase in Fermi energy, we constructed Ru-doped LiFe Ru PO/C through the sol-gel preparation technology as cathode materials for Li-ion batteries. As a result, LFP-1 ( = 0.01) delivers excellent specific capacities of 162.6 and 110.6 mA h g under 0.1 and 10 C, respectively. At the same time, LFP-1 emerges with excellent cycling performance, with a capacity retention of up to 95.6% after 300 cycles at 5 C. Ru doping is beneficial for improving the lithium diffusion coefficient and electrical conductivity, therefore strongly increasing electrochemical performance. This work represents a significant addition to exploring a new class of lithium iron phosphates with excellent performance in new energy storage and transition systems.
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http://dx.doi.org/10.1021/acsomega.1c00595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190806PMC
June 2021

Sophoraflavanone M, a prenylated flavonoid from Sophora flavescens Ait., suppresses pro-inflammatory mediators through both NF-κB and JNK/AP-1 signaling pathways in LPS-primed macrophages.

Eur J Pharmacol 2021 Jun 9;907:174246. Epub 2021 Jun 9.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

(2R)-3α,7,4'-trihydroxy-5-methoxy-8-(γ,γ-dimethylallyl)-flavanone is a prenylated flavonoid isolated from the anti-inflammatory herb Sophora flavescens Ait. We firstly named it sophoraflavanone M (SFM) in accordance with trivial names of related constitutes from this plant. Although various studies investigated the anti-inflammatory properties of prenylated flavonoids from Sophora flavescens Ait., that of SFM remains unclear and is yet to be determined. In the current study, we assessed the anti-inflammatory effects of SFM in LPS-induced in vivo and in vitro models. In the serum of endotoxemia mice, SFM significantly suppressed LPS-elevated inflammatory cytokines. Furthermore, at nontoxic concentrations, SFM reduced LPS-induced production of inflammatory mediators NO, IL-6, TNF-α, and MCP-1 in mouse primary peritoneal macrophages. Accordingly, in LPS-primed RAW264.7 cell line, it also inhibited these mediators' expression at both transcriptional and translational levels without cytotoxicity. Mechanistically, SFM is found to concurrently inhibit two important inflammatory signaling pathways, NF-κB and JNK/AP-1. SFM restrained phosphorylation and degradation of IκBα as well as the subsequent p65 translocation to dampen NF-κB activity. Meanwhile, it also suppressed JNK phosphorylation to inhibit the transcriptional activity of AP-1. These results provide material basis for traditional application of the anti-inflammatory herb Sophora flavescens Ait. and suggest SFM is a promising natural candidate for alleviating inflammatory conditions.
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http://dx.doi.org/10.1016/j.ejphar.2021.174246DOI Listing
June 2021

LncRNA SAMMSON Overexpression Suppresses Vascular Smooth Muscle Cell Proliferation via Inhibiting miR-130a Maturation to Participate in Intracranial Aneurysm.

Neuropsychiatr Dis Treat 2021 4;17:1793-1799. Epub 2021 Jun 4.

Department of Neurosurgery West China Hospital, Sichuan University, Chengdu Province, 610041, People's Republic of China.

Background: MiR-130a is a recently identified critical player in vascular smooth muscle cell (VSMC) proliferation, which participates in intracranial aneurysm (IA). However, the involvement of miR-130a in IA and its upstream regulator are unknown. Our preliminary sequencing analysis revealed a close correlation between miR-130a and lncRNA SAMMSON across IA samples. Therefore, we further studied the crosstalk between SAMMSON and miR-130a in IA.

Methods: SAMMSON and miR-130a expression were measured using RT-qPCR. SAMMSON subcellular location was analyzed with nuclear fractionation assay. Their direct interaction was explored with RNA pull-down assay. The role of SAMMSON in miR-130a maturation was studied with overexpression analysis. VSMC cell proliferation was analyzed with BrdU assay.

Results: SAMMSON and premature miR-130a were deregulated in IA, while mature miR-130a was upregulated in IA. SAMMSON is localized in both the nucleus and cytoplasm, and direct interaction between SAMMSON and miR-130a was observed. SAMMSON overexpression suppressed miR-130a maturation in VSMCs and reduced the enhancing effects of miR-130a on VSMC cell proliferation.

Conclusion: SAMMSON is overexpressed in IA and suppresses VSMC proliferation via inhibiting miR-130a maturation.
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http://dx.doi.org/10.2147/NDT.S311499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187098PMC
June 2021

Repeated 3,3-Dimethyl-1-butanol exposure alters social dominance in adult mice.

Neurosci Lett 2021 Jul 9;758:136006. Epub 2021 Jun 9.

Neuroscience Research Center, Institute of Medical and Health Science of HeBMU, Hebei Medical University, Shijiazhuang 050017, China; Hebei Key Laboratory of Neurophysiology, Hebei Medicinal University, Shijiazhuang 050017, China; Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei Medicinal University, Shijiazhuang 050017, China; Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical Sciences, Shijiazhuang 050017, China. Electronic address:

The influence of gut microbiota on brain function and brain disorders has been attracted more and more attention. Trimethylamine N-oxide (TMAO), an indirect metabolite of gut microbiota, has been linked to aging, cognitive impairment, and other brain disorders. However, the relationship between TMAO and social behaviors are still poorly understood. Adult male mice were exposed to drinking water containing 3,3- Dimethyl-1-butanol (DMB), an indirect inhibitors of TMAO, for 21 continuous days followed by a series of behavioral tests to detect the effect of DMB exposure on social behaviors, mainly including social dominance test (SDT), bedding preference test (BP), sexual preference test (SP), social interaction test (SI), open field test (OFT), tail suspension test (TST), forced swim test (FST), novelty suppressed feeding test (NSF), and novel object recognition (NOR) task. In the SDT, compared with the control group, the mice treated with DMB (both 0.2% and 1.0%), both high-ranked and low-ranked mice, showed a reduction in the number of victories. There is no statistical difference on sexual preference, anxiety, depression-like behavior phenotype, and memory formation. In conclusion, the present findings provide direct evidence, for the first time, that repeated DMB exposure produces significant effects on social dominance of adult mice, without any effects on sexual preference, anxiety, depression-like behavior phenotype or memory formation, highlighting the regulatory effects of gut-brain interaction on social behaviors.
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http://dx.doi.org/10.1016/j.neulet.2021.136006DOI Listing
July 2021

[A case of tuberous sclerosis complex due to a novel splicing variant of TSC2 gene].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):553-556

Center for Reproductive Medicine Research, Shandong University; National Research Center for Assisted Reproductive Technology and Reproductive Genetics; Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250001, China.

Objective: To explore the genetic basis for a patient with tuberous sclerosis complex.

Methods: Genomic DNA was extracted from peripheral blood samples from members of his family and 100 unrelated healthy controls. The proband was subjected to next-generation sequencing, and candidate variant was confirmed by multiple ligation-dependent probe amplification (MLPA) and Sanger sequencing. Reverse transcription-PCR (RT-PCR) was carried out to determine the relative mRNA expression in the proband.

Results: The patient was found to harbor a c.2355+1G>C splicing variant of the TSC2 gene. Sequencing of cDNA confirmed that 62 bases have been inserted into the 3' end of exon 21, which has caused a frameshift producing a truncated protein.

Conclusion: The novel splicing variant c.2355+1G>C of the TSC2 gene probably underlay the TSC in the proband. Above finding has expanded the variant spectrum of TSC2 and provided a basis for preimplantation genetic testing and/or prenatal diagnosis.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200211-00072DOI Listing
June 2021

Upregulation of long noncoding RNA W42 promotes tumor development by binding with DBN1 in hepatocellular carcinoma.

World J Gastroenterol 2021 May;27(20):2586-2602

Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China.

Background: Hepatocellular carcinoma (HCC) is a malignancy found globally. Accumulating studies have shown that long noncoding RNAs (lncRNAs) play critical roles in HCC. However, the function of lncRNA in HCC remains poorly understood.

Aim: To understand the effect of lncRNA W42 on HCC and dissect the underlying molecular mechanisms.

Methods: We measured the expression of lncRNA W42 in HCC tissues and cells (Huh7 and SMMC-7721) by quantitative reverse transcriptase polymerase chain reaction. Receiver operating characteristic curves were used to assess the sensitivity and specificity of lncRNA W42 expression. HCC cells were transfected with pcDNA3.1-lncRNA W42 or shRNA-lncRNA W42. Cell functions were detected by cell counting Kit-8 (CCK-8), colony formation, flow cytometry and Transwell assays. The interaction of lncRNA W42 and DBN1 was confirmed by RNA immunoprecipitation and RNA pull down assays. An HCC xenograft model was used to assess the role of lncRNA W42 on tumor growth . The Kaplan-Meier curve was used to evaluate the overall survival and recurrence-free survival after surgery in patients with HCC.

Results: In this study, we identified a novel lncRNA (lncRNA W42), and investigated its biological functions and clinical significance in HCC. LncRNA W42 expression was upregulated in HCC tissues and cells. Overexpression of lncRNA W42 notably promoted the proliferative and invasion of HCC, and inhibited cell apoptosis. LncRNA W42 directly bound to DBN1 and activated the downstream pathway. LncRNA W42 knockdown suppressed HCC xenograft tumor growth . The clinical investigation revealed that HCC patients with high lncRNA W42 expression exhibited shorter survival times.

Conclusion: and results suggested that the novel lncRNA W42, which is upregulated in HCC, may serve as a potential candidate prognostic biomarker and therapeutic target in HCC patients.
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http://dx.doi.org/10.3748/wjg.v27.i20.2586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160624PMC
May 2021

polysaccharides alleviate cognitive decline in aging model mice by restoring the gut microbiota-brain axis.

Aging (Albany NY) 2021 Jun 3;13(11):15320-15335. Epub 2021 Jun 3.

Inner Mongolia Medical University, Hohhot 010110, China.

Recent evidence suggests alterations in the gut microbiota-brain axis may drive cognitive impairment with aging. In the present study, we observed that prolonged administration of D-galactose to mice induced cognitive decline, gut microbial dysbiosis, peripheral inflammation, and oxidative stress. In this model of age-related cognitive decline, polysaccharides (CDPS) improved cognitive function in D-galactose-treated mice by restoring gut microbial homeostasis, thereby reducing oxidative stress and peripheral inflammation. The beneficial effects of CDPS in these aging model mice were abolished through ablation of gut microbiota with antibiotics or immunosuppression with cyclophosphamide. Serum metabolomic profiling showed that levels of creatinine, valine, L-methionine, o-Toluidine, N-ethylaniline, uric acid and proline were all altered in the aging model mice, but were restored by CDPS. These findings demonstrated that CDPS improves cognitive function in a D-galactose-induced aging model in mice by restoring homeostasis of the gut microbiota-brain axis, which alleviated an amino acid imbalance, peripheral inflammation, and oxidative stress. CDPS thus shows therapeutic potential for patients with memory and learning disorders, especially those related to gut microbial dysbiosis.
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http://dx.doi.org/10.18632/aging.203090DOI Listing
June 2021

Metabolomics analysis of the soapberry (Sapindus mukorossi Gaertn.) pericarp during fruit development and ripening based on UHPLC-HRMS.

Sci Rep 2021 Jun 2;11(1):11657. Epub 2021 Jun 2.

Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, 35 E Qinghua Road, Beijing, 100083, China.

Soapberry (Sapindus mukorossi Gaertn.) is a multi-functional tree with widespread application in toiletries, biomedicine, biomass energy, and landscaping. The pericarp of soapberry can be used as a medicine or detergent. However, there is currently no systematic study on the chemical constituents of soapberry pericarp during fruit development and ripening, and the dynamic changes in these constituents still unclear. In this study, a non-targeted metabolomics approach using ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) was used to comprehensively profile the variations in metabolites in the soapberry pericarp at eight fruit growth stages. The metabolome coverage of UHPLC-HRMS on a HILIC column was higher than that of a C18 column. A total of 111 metabolites were putatively annotated. Principal component analysis and hierarchical clustering analysis of pericarp metabolic composition revealed clear metabolic shifts from early (S1-S2) to late (S3-S5) development stages to fruit ripening stages (S6-S8). Furthermore, pairwise comparison identified 57 differential metabolites that were involved in 18 KEGG pathways. Early fruit development stages (S1-S2) were characterized by high levels of key fatty acids, nucleotides, organic acids, and phosphorylated intermediates, whereas fruit ripening stages (S6-S8) were characterized by high contents of bioactive and valuable metabolites, such as troxipide, vorinostat, furamizole, alpha-tocopherol quinone, luteolin, and sucrose. S8 (fully developed and mature stage) was the most suitable stage for fruit harvesting to utilize the pericarp. To the best of our knowledge, this was the first metabolomics study of the soapberry pericarp during whole fruit growth. The results could offer valuable information for harvesting, processing, and application of soapberry pericarp, as well as highlight the metabolites that could mediate the biological activity or properties of this medicinal plant.
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http://dx.doi.org/10.1038/s41598-021-91143-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172880PMC
June 2021

Effect of Co Doping on Electrocatalytic Performance of Co-NiS/CoS Heterostructures.

Nanomaterials (Basel) 2021 May 8;11(5). Epub 2021 May 8.

Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

There are abundant water resources in nature, and hydrogen production from electrolyzed water can be one of the main ways to obtain green and sustainable energy. Traditional water electrolysis uses precious metals as catalysts, but it is difficult to apply in massive volumes due to low reserves and high prices. It is still a challenge to develop hydrogen electrocatalysts with excellent performance but low cost to further improve the efficiency of hydrogen production. This article reported a potential candidate, the Co-NiS/CoS (material is based on NiS, and after Co doping, The NiS/CoS heterostructure is formed) heterostructures, prepared by hydrothermal method with carbon paper as the substrate. In a 0.5 M sulfuric acid solution, the hydrogen evolution reaction with Co-NiS/CoS as the electrode showed excellent catalytic performance. When the Co (Cobalt) doping concentration is increased to 27%, the overpotential is -133.3 mV, which is a drop of 81 mV compared with -214.3 mV when it is not doped. The heterostructure formed after doping also has good stability. After 800 CV cycles, the difference in overpotential is only 3 mV. The significant improvement of the catalytic performance can be attributed to the significant changes in the crystal structure and properties of the doped heterostructures, which provide an effective method for efficient electrocatalytic hydrogen production.
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http://dx.doi.org/10.3390/nano11051245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150845PMC
May 2021

Genomes of Anguillid Herpesvirus 1 Strains Reveal Evolutionary Disparities and Low Genetic Diversity in the Genus .

Microorganisms 2021 May 5;9(5). Epub 2021 May 5.

Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.

Anguillid herpesvirus 1 (AngHV-1) is a pathogen of eels and a member of the genus in the family . We have compared the biological and genomic features of different AngHV-1 strains, focusing on their growth kinetics in vitro and genetic content, diversity, and recombination. Comparisons based on three core genes conserved among alloherpesviruses revealed that AngHV-1 exhibits a slower rate of change and less positive selection than other cypriniviruses. We propose that this may be linked to major differences in host species and corresponding epidemiological circumstances. Efforts to derive evolutionary rate estimates for cypriniviruses under various theoretical models were ultimately unrewarding. We highlight the potential value of future collaborative efforts towards generating short-term evolutionary rate estimates based on known sequence sampling dates. Finally, we revealed that there is significantly less genetic diversity in core gene sequences within cyprinivirus species clades compared to species in the family . This suggests that cyprinivirus species may have undergone much more vigorous purifying selection post species clade divergence. We discuss whether this may be linked to biological and anthropogenic factors or to sampling bias, and we propose that the comparison of short-term evolutionary rates between species may provide further insights into these differences.
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http://dx.doi.org/10.3390/microorganisms9050998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148134PMC
May 2021

Spontaneous Patterning during Frontal Polymerization.

ACS Cent Sci 2021 Apr 24;7(4):603-612. Epub 2021 Mar 24.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States.

Complex patterns integral to the structure and function of biological materials arise spontaneously during morphogenesis. In contrast, functional patterns in synthetic materials are typically created through multistep manufacturing processes, limiting accessibility to spatially varying materials systems. Here, we harness rapid reaction-thermal transport during frontal polymerization to drive the emergence of spatially varying patterns during the synthesis of engineering polymers. Tuning of the reaction kinetics and thermal transport enables internal feedback control over thermal gradients to spontaneously pattern morphological, chemical, optical, and mechanical properties of structural materials. We achieve patterned regions with two orders of magnitude change in modulus in poly(cyclooctadiene) and 20 °C change in glass transition temperature in poly(dicyclopentadiene). Our results suggest a facile route to patterned structural materials with complex microstructures without the need for masks, molds, or printers utilized in conventional manufacturing. Moreover, we envision that more sophisticated control of reaction-transport driven fronts may enable spontaneous growth of structures and patterns in synthetic materials, inaccessible by traditional manufacturing approaches.
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http://dx.doi.org/10.1021/acscentsci.1c00110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155460PMC
April 2021

A novel decision tree model based on chromosome imbalances in cell-free DNA and CA-125 in the differential diagnosis of ovarian cancer.

Int J Biol Markers 2021 May 31:1724600821992356. Epub 2021 May 31.

Department of Gynecology, Qingdao Municipal Hospital, Qingdao, Shandong Province, China.

Objective: CA-125 is widely used as biomarker of ovarian cancer. However, CA-125 suffers low accuracy. We developed a hybrid analytical model, the Ovarian Cancer Decision Tree (OCDT), employing a two-layer decision tree, which considers genetic alteration information from cell-free DNA along with CA-125 value to distinguish malignant tumors from benign tumors.

Methods: We consider major copy number alterations at whole chromosome and chromosome-arm level as the main feature of our detection model. Fifty-eight patients diagnosed with malignant tumors, 66 with borderline tumors, and 10 with benign tumors were enrolled.

Results: Genetic analysis revealed significant arm-level imbalances in most malignant tumors, especially in high-grade serous cancers in which 12 chromosome arms with significant aneuploidy (<0.01) were identified, including 7 arms with significant gains and 5 with significant losses. The area under receiver operating characteristic curve (AUC) was 0.8985 for copy number variations analysis, compared to 0.8751 of CA125. The OCDT was generated with a cancerous score (CScore) threshold of 5.18 for the first level, and a CA-125 value of 103.1 for the second level. Our most optimized OCDT model achieved an AUC of 0.975.

Conclusions: The results suggested that genetic variations extracted from cfDNA can be combined with CA-125, and together improved the differential diagnosis of malignant from benign ovarian tumors. The model would aid in the pre-operative assessment of women with adnexal masses. Future clinical trials need to be conducted to further evaluate the value of CScore in clinical settings and search for the optimal threshold for malignancy detection.
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http://dx.doi.org/10.1177/1724600821992356DOI Listing
May 2021

Effects of two different emotion-inducing methods on the emotional memory of non-clinically depressed individuals.

PLoS One 2021 28;16(5):e0249863. Epub 2021 May 28.

Key Laboratory of Brain, Cognition and Education Sciences, South China Normal University, Ministry of Education, Guangzhou, Guangdong, China.

In the study of emotional memory bias in depressed individuals, most previous studies have used emotional materials, but there were significant differences in the effects of different emotion-inducing methods on face memory. In the present study, two experiments were conducted to explore the effects of different emotion-inducing methods on memory between healthy participants and non-clinically depressed participants. The results from experiment 1 showed that when feedback was used as induction, the memory performance of the non-clinical depression group was significantly higher than that of the healthy group under the condition of negative feedback. Under positive and neutral feedback, there were no significant differences between the two groups. In experiment 2, when emotional materials were used as a mode of induction, no significantly difference in each emotional condition between the healthy and depressed groups was found. The results of the present study show that different methods of emotional induction have different effects on depressed participants. Compared with the emotion induced by the emotional material, the non-clinical depressed participants had a better memory effect induced by negative emotional events.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249863PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162709PMC
May 2021

Hyperoxalemia Leads to Oxidative Stress in Endothelial Cells and Mice with Chronic Kidney Disease.

Kidney Blood Press Res 2021 May 27:1-10. Epub 2021 May 27.

Kidney Institute, Department of Nephrology, Changzheng Hospital, Naval Medical University, Shanghai, China.

Introduction: Cardiovascular disease is the most common cause of morbidity and mortality in patients with ESRD. In addition to phosphate overload, oxalate, a common uremic toxin, is also involved in vascular calcification in patients with ESRD. The present study investigated the role and mechanism of hyperoxalemia in vascular calcification in mice with uremia.

Methods: A uremic atherosclerosis (UA) model was established by left renal excision and right renal electrocoagulation in apoE-/- mice to investigate the relationship between oxalate loading and vascular calcification. After 12 weeks, serum and vascular levels of oxalate, vascular calcification, inflammatory factors (TNF-α and IL-6), oxidative stress markers (malondialdehyde [MDA], and advanced oxidation protein products [AOPP]) were assessed in UA mice. The oral oxalate-degrading microbe Oxalobacter formigenes (O. formigenes) was used to evaluate the effect of a reduction in oxalate levels on vascular calcification. The mechanism underlying the effect of oxalate loading on vascular calcification was assessed in cultured human aortic endothelial cells (HAECs) and human aortic smooth muscle cells (HASMCs).

Results: Serum oxalate levels were significantly increased in UA mice. Compared to the control mice, UA mice developed more areas of aortic calcification and showed significant increases in aortic oxalate levels and serum levels of oxidative stress markers and inflammatory factors. The correlation analysis showed that serum oxalate levels were positively correlated with the vascular oxalate levels and serum MDA, AOPP, and TNF-α levels, and negatively correlated with superoxide dismutase activity. The O. formigenes intervention decreased serum and vascular oxalate levels, while did not improve vascular calcification significantly. In addition, systemic inflammation and oxidative stress were also improved in the O. formigenes group. In vitro, high concentrations of oxalate dose-dependently increased oxidative stress and inflammatory factor expression in HAECs, but not in HASMCs.

Conclusions: Our results indicated that hyperoxalemia led to the systemic inflammation and the activation of oxidative stress. The reduction in oxalate levels by O. formigenes might be a promising treatment for the prevention of oxalate deposition in calcified areas of patients with ESRD.
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http://dx.doi.org/10.1159/000516013DOI Listing
May 2021

Present Situation and Research Progress of Kidney Function Recoverability Evaluation of Acute Kidney Injury Patient.

Int J Gen Med 2021 18;14:1919-1925. Epub 2021 May 18.

Department of Critical Care Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People's Republic of China.

Acute kidney injury (AKI) is a critical illness in clinic. The guideline recommendation of kidney disease for improving global outcomes regards urine volume and creatinine as standards to evaluate kidney functions. However, urine volume and creatinine have a certain delay for kidney function evaluation, and these would be interfered by many factors. Whether the renal function of AKI patients can recover is very important, which affects the quality of life of patients. Therefore, the present study reviews the application situation and research progress of the recoverability evaluation of AKI patient kidney function from three aspects: conventional indexes, biomarkers, and imaging methods of kidney function.
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http://dx.doi.org/10.2147/IJGM.S303348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140891PMC
May 2021

Small extracellular vesicles in combination with sleep-related circRNA3503: A targeted therapeutic agent with injectable thermosensitive hydrogel to prevent osteoarthritis.

Bioact Mater 2021 Dec 6;6(12):4455-4469. Epub 2021 May 6.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, 200233, China.

Osteoarthritis (OA), characterized by chondrocyte apoptosis and disturbance of the balance between catabolism and anabolism of the extracellular matrix (ECM), is the most common age-related degenerative joint disease worldwide. As sleep has been found to be beneficial for cartilage repair, and circular RNAs (circRNAs) have been demonstrated to be involved in the pathogenesis of OA, we performed RNA sequencing (RNA-seq), and found circRNA3503 was significantly increased after melatonin (MT)-induced cell sleep. Upregulation of circRNA3503 expression completely rescued the effects of interleukin-1β (IL-1β), which was used to simulate OA, on apoptosis, ECM degradation- and synthesis-related genes. Mechanistically, circRNA3503 acted as a sponge of hsa-miR-181c-3p and hsa-let-7b-3p. Moreover, as we previously showed that small extracellular vesicles (sEVs) derived from synovium mesenchymal stem cells (SMSCs) can not only successfully deliver nucleic acids to chondrocytes, but also effectively promote chondrocyte proliferation and migration, we assessed the feasibility of sEVs in combination with sleep-related circRNA3503 as an OA therapy. We successfully produced and isolated circRNA3503-loaded sEVs (circRNA3503-OE-sEVs) from SMSCs. Then, poly(D,l-lactide)-b-poly(ethylene glycol)-b-poly(D,l-lactide) (PDLLA-PEG-PDLLA, PLEL) triblock copolymer gels were used as carriers of sEVs. Through and experiments, [email protected] were shown to be a highly-effective therapeutic strategy to prevent OA progression. Through multiple pathways, circRNA3503-OE-sEVs alleviated inflammation-induced apoptosis and the imbalance between ECM synthesis and ECM degradation by acting as a sponge of hsa-miR-181c-3p and hsa-let-7b-3p. In addition, circRNA3503-OE-sEVs promoted chondrocyte renewal to alleviate the progressive loss of chondrocytes. Our results highlight the potential of [email protected] for preventing OA progression.
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http://dx.doi.org/10.1016/j.bioactmat.2021.04.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120802PMC
December 2021

Extracellular vesicles derived from myocardial infarction plasma inhibit BMSCs apoptosis and enhance cardiac function via AKT signaling pathway.

Int Immunopharmacol 2021 Jul 19;96:107730. Epub 2021 May 19.

Department of Cardiovascular Surgery, Beijing Anzhen Hospital of Capital Medical University and Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. Electronic address:

This study aimed to investigate whether extracellular vesicles (EVs) secreted in myocardial infarction (MI) plasma could protect against apoptosis of bone marrow mesenchymal stem cells (BMSCs) following hypoxia or serum deprivation in vitro and improve cardiac function following MI in vivo. The plasma samples were taken from female rats 24 h after MI. EVs were obtained and co-cultured with BMSCs. We found that EVs could be taken up by BMSCs. Co-culturing with EVs attenuated hypoxia-induced apoptosis of BMSCs in EVs in a dose-dependent manner, which was reversed by the pharmacological inhibition of AKT signaling. Co-culturing with EVs improved transplantation efficiency and blunted MI-induced apoptosis of BMSCs in vivo. Furthermore, transplantation of BMSCs together with EVs can effectively promote the increase in capillary density both at the border and central zone of myocardium and ameliorate myocardial remodeling in MI rats. BMSCs and EVs transplantation treatment exhibited significant improvements in ejection fraction, fraction shortening, left ventricular end-diastolic dimensions, and left ventricular end-systolic dimensions, as evaluated by echocardiography four weeks after MI in rats. Finally, levels of differentiation- and apoptosis-related microRNAs expression in EVs that may mediate these effects were also identified by microarray and quantitative real-time PCR. In conclusion, the present results suggest a potential role of plasma-derived EVs in decreasing apoptosis of BMSCs by activating AKT signaling, promoting angiogenesis, ameliorating myocardial remodeling, and improving cardiac function in MI rats. EV application may be a novel option to ameliorate the therapeutic efficiency of BMSCs to improve cardiac function following MI.
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http://dx.doi.org/10.1016/j.intimp.2021.107730DOI Listing
July 2021

The Value of Gene Detection and Skin Biopsy in the Diagnosis of Neuronal Intranuclear Inclusion Disease.

Front Neurol 2021 4;12:624321. Epub 2021 May 4.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

The clinical manifestations of neuronal intranuclear inclusion disease (NIID) are heterogeneous, and the premortem diagnosis is mainly based on skin biopsy findings. Abnormal GGC repeat expansions in was recently identified in familial and sporadic NIID. The comparison of diagnostic value between abnormal GGC repeat expansions of and skin biopsy has not been conducted yet. In this study, skin biopsy was performed in 10 suspected adult NIID patients with clinical and imaging manifestations, and GGC repeat size in was also screened by repeat primed-PCR and GC-rich PCR. We found that five cases had ubiquitin-immunolabelling intranuclear inclusion bodies by skin biopsy, and all of them were identified with abnormal GGC repeat expansions in , among whom four patients showed typical linear hyperintensity at corticomedullary junction on DWI. Five (5/10) NIID patients were diagnosed by combination of gene detection, skin biopsy or combination of , and typical MRI findings. The diagnostic performance of gene detection was highly consistent with that of skin biopsy ( = 1). The unexplained headache was firstly reported as a new early phenotype of NIID. These findings indicate that gene detection is needed to be a supplement in the diagnose flow of NIID and also may be used as an alternative method to skin biopsy especially in Asian population.
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http://dx.doi.org/10.3389/fneur.2021.624321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129528PMC
May 2021

Functional Properties and Structural Characteristics of Starch-Fatty Acid Complexes Prepared at High Temperature.

J Agric Food Chem 2021 May 19. Epub 2021 May 19.

College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100 Xianyang, Shaanxi, P. R. China.

The effects of fatty acid type (myristic, palmitic, stearic, oleic, linoleic, and linolenic acid) on the characteristics of starch-lipid complexes under high temperature were investigated. Fatty acids with a shorter carbon chain or a greater number of double bonds contributed to the formation of V-type starch-lipid complexes. The thermostability of starch-unsaturated fatty acid (UFA) complexes prepared at high temperature was increased compared with those obtained at lower temperature. Resistant starch (RS) contents and melting temperatures had a strong significant positive correlation. Complexes with better thermostability were more resistant to enzymatic hydrolysis. Among them, the starch-stearic acid complexes possessed the highest RS content. The paste of starch-linolenic acid complexes had the lowest internal friction and the strongest thixotropy. The broken of double bonds in UFAs probably accounted for the increased starch-lipid complexes. The crystalline, thermal, rheological, and digestion properties of samples treated at high temperature were significantly affected.
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http://dx.doi.org/10.1021/acs.jafc.1c00110DOI Listing
May 2021

Ruxolitinib exerts neuroprotection via repressing ferroptosis in a mouse model of traumatic brain injury.

Exp Neurol 2021 Aug 12;342:113762. Epub 2021 May 12.

Department of Forensic Medicine, School of basic medicine and Biological Sciences, Affiliated Guangji Hospital, Soochow University, China. Electronic address:

Traumatic brain injury (TBI) is a major cause of death and disability worldwide. Various forms of cells death are involved in the pathological process of TBI, without exception to ferroptosis, which is mainly triggered by iron-dependent lipid peroxidation. Although there have been studies on ferroptosis and TBI, the effect of ruxolitinib (Ruxo), one type of FDA approved drugs for treating myelofibrosis, on the process of ferroptosis post-TBI is remained non-elucidated. Therefore, using a controlled cortical impact device to establish the mouse TBI model, we examined the effect of Ruxo on TBI-induced ferroptosis, in which the inhibitor of ferroptosis, Ferrostatin-1 (Fer-1) was used as a positive control. Moreover, we also respectively explored the effects of these two interventions on neurological deficits caused by TBI. We firstly examined the expression patterns of ferroptosis-related markers at protein level at different time points after TBI. And based on the expression changes of these markers, we chose 12 h post-TBI to prove the effect of Ruxo on ferroptosis. Importantly, we found the intensely inhibitory effect of Ruxo on ferroptosis, which is in parallel with the results obtained after Fer-1-treatment. In addition, these two treatments both alleviated the content of brain water and degree of neurodegeneration in the acute phase of TBI. Finally, we further confirmed the neuroprotective effect of Ruxo or Fer-1 via the wire-grip test, Morris water maze and open field test, respectively. Thereafter, the lesion volume and iron deposition were also measured to certificate their effects on the long-term outcomes of TBI. Our results ultimately demonstrate that inhibiting ferroptosis exerts neuroprotection, and this is another neuroprotective mechanism of Ruxo on TBI.
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http://dx.doi.org/10.1016/j.expneurol.2021.113762DOI Listing
August 2021

Twin curvilinear vortex beams.

Opt Express 2021 Apr;29(9):14112-14125

We report on a novel curvilinear optical vortex beam named twin curvilinear vortex beams (TCVBs) with intensity and phase distribution along a pair of two- or three-dimensional curves, both of which share the same shape and the same topological charge. The TCVBs also possess the character of perfect optical vortex, namely having a size independent of topological charge. We theoretically demonstrate that a TCVB rather than a single-curve vortex beam can be created by the Fourier transform of a cylindrically polarized beam. The behavior of TCVBs generated through our method is investigated by simulation and experiment, including interference experiments for identifying the vortex property of the TCVBs. The TCVBs may find applications in optical tweezers, such as trapping low refractive index particles in the dark region between two curves and driving them moving along the curvilinear trajectory.
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http://dx.doi.org/10.1364/OE.423803DOI Listing
April 2021

All-Inorganic Quantum-Dot LEDs Based on a Phase-Stabilized α-CsPbI3 Perovskite.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario, M5S 3G4, Canada.

The all-inorganic nature of CsPbI perovskites allows to enhance stability in perovskite devices. Research efforts have led to improved stability of the black phase in CsPbI films; however, these strategies-including strain and doping-are based on organic-ligand-capped perovskites, which prevent perovskites from forming the close-packed quantum dot (QD) solids necessary to achieve high charge and thermal transport. We developed an inorganic ligand exchange that leads to CsPbI QD films with superior phase stability and increased thermal transport. The atomic-ligand-exchanged QD films, once mechanically coupled, exhibit improved phase stability, and we link this to distributing strain across the film. Operando measurements of the temperature of the LEDs indicate that KI-exchanged QD films exhibit increased thermal transport compared to controls that rely on organic ligands. The LEDs exhibit a maximum EQE of 23 % with an electroluminescence emission centered at 640 nm (FWHM: ≈31 nm). These red LEDs provide an operating half-lifetime of 10 h (luminance of 200 cd m ) and an operating stability that is 6× higher than that of control devices.
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http://dx.doi.org/10.1002/anie.202104812DOI Listing
May 2021

Augmented reality navigation method for recontouring surgery of craniofacial fibrous dysplasia.

Sci Rep 2021 May 11;11(1):10043. Epub 2021 May 11.

Department of Oral and Craniomaxillofacial Surgery, Shanghai 9Th People's Hospital, Shanghai Jiaotong University College of Medicine, Shanghai, China.

The objective of this study is to introduce the application of augmented reality (AR) navigation system developed by the authors in recontouring surgery of craniofacial fibrous dysplasia. Five consecutive patients with craniofacial fibrous dysplasia were enrolled. Through three-dimensional (3D) simulation, a virtual plan was designed to reconstruct the normal anatomical contour of the deformed region. Surgical recontouring was achieved with the assistance of the AR navigation system. The accuracy of the surgical procedure was assessed by superimposing the post-operative 3D craniomaxillofacial model onto the virtual plan. The pre-operative preparation time and operation time were also counted. In all patients, AR navigation was performed successfully, with a mean ± SD of the errors of 1.442 ± 0.234 mm. The operative time of the patients ranged from 60 to 80 min. The pre-operative preparation time was 20 min for each patient. All the patients showed uneventful healing without any complications, in addition to satisfaction with the post-operative aesthetics. Using our AR navigation system in recontouring surgery can provide surgeons with a comprehensive and intuitive view of the recontouring border, as well as the depth, in real time. This method could improve the efficiency and safety of craniofacial fibrous dysplasia recontouring procedures.
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http://dx.doi.org/10.1038/s41598-021-88860-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113548PMC
May 2021

Redox-Mediated Reversible Supramolecular Assemblies Driven by Switch and Interplay of Peptide Secondary Structures.

Biomacromolecules 2021 Jun 7;22(6):2563-2572. Epub 2021 May 7.

CAS Center of Excellence for Nanoscience, Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Sciences, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.

The construction of reversible supramolecular self-assembly remains a significant challenge. Here, we demonstrate the redox-triggered reversible supramolecular self-assembly governed by the "check and balance" of two secondary conformations within a brushlike peptide-selenopolypeptide conjugate. The conjugate constitutes a polypeptide backbone whose side chain contains selenoether functional moieties and double bonds to be readily grafted with β-sheet-prone short-peptide NapFFC. The backbone of the conjugate initially assumes a robust and rigid α-helical conformation, which inhibits the supramolecular assembly of the short peptide in the side chain and yields an overall irregular aggregate morphology under native/reduced conditions. Upon oxidation of the selenoether to more hydrophilic selenoxide, the backbone helix switches to a flexible and disordered conformation, which unleashes the side-chain NapFFC self-assembly into nanofibrils via the adoption of β-sheet conformation. The reversible switch of the supramolecular morphology enables efficient loading and tumor-microenvironment-triggered release of anticancer drugs for cancer treatment with satisfactory efficacy and biocompatibility. The interplay and interaction between two well-defined secondary structures within one scaffold offer tremendous opportunity for the design and construction of functional supramolecular biomaterials.
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http://dx.doi.org/10.1021/acs.biomac.1c00300DOI Listing
June 2021